               SEQUENCE LISTING

<110> Nestec S.A.

<120> Natural derivative of the Lactobacillus johnsonii strain CNCM I-1225, deficient in D-lactic acid production and with a further improved immune profile

<150> EP11160144.9
<151> 2011-03-29

<160> 7

<170> BiSSAP 1.0

<210> 1
<211> 1080
<212> DNA
<213> Lactobacillus johnsonii

<220> 
<221> source
<222> 1..1080
<223> /mol_type="DNA"
      /organism="Lactobacillus johnsonii"

<400> 1
aaagacggtc atactattaa ttttgacgga ggtttaattt atgacaaaga tttttgctta      60

cgctattcgt aaagatgaag aacctttctt aaacgaatgg aaagatgcac acaaggatat     120

tgaagttgaa tacactgaca agcttttagc ccctgaaact gctaaattag ctaagggtgc     180

tgacggtgtt gttgtttacc aacaattaga ctacactcct gaaactcttc aagctttagc     240

tgatgctggc gtaactaaga tgtcattacg taacgttggt gttgacaaca tcgacatgga     300

caaggctaaa gaattaggct ttgaaatcac taacgttcct gtatactctc ctgacgcaat     360

tgctgaacat gctgcaattc aagctgctcg cgtactacgt caagataagc gtatggatga     420

aaagatggct aagcgcgact tacgctgggc acctactatt ggtcgtgaag ttcgtgacca     480

agttgttggt gttgtaggta ctggtcacat cggtcaagta tttatgaaga ttatggaagg     540

ctttggcgca aaagttattg cttacgatat cttcaagaac ccagaacttg aaaagaaggg     600

ttactacgtt gactcacttg atgacttata caagcaagct gatgtaattt cacttcacgt     660

tccagatgtt ccagcaaacg ttcacatgat taatgatgaa tcaatcgcta agatgaaaga     720

tggcgttgta atcgtaaact gctcacgtgg tccacttgtt gacactgacg ctgttatccg     780

cggcttagat tctggtaaga tctttggttt cgtaatggac acttacgaag gtgaagttgg     840

tgtatttaac gaagattggg aaggtaaaga attcccagat gctcgtttag ctgacttaat     900

cgatcgtcca aatgtattgg taactccaca tactgctttc tacactactc atgctgtacg     960

taacatggta actaaagcat ttgacaacaa cttaaagatg atcaacggtg aaaaaccaga    1020

ttctccagtt gctttggaca agaacaagtt ctaaaaatca aattatgata atcaaaaaga    1080


<210> 2
<211> 337
<212> PRT
<213> Lactobacillus johnsonii

<220> 
<221> SOURCE
<222> 1..337
<223> /mol_type="protein"
      /organism="Lactobacillus johnsonii"

<400> 2
Met Thr Lys Ile Phe Ala Tyr Ala Ile Arg Lys Asp Glu Glu Pro Phe 
1               5                   10                   15    
Leu Asn Glu Trp Lys Asp Ala His Lys Asp Ile Glu Val Glu Tyr Thr 
            20                   25                  30        
Asp Lys Leu Leu Ala Pro Glu Thr Ala Lys Leu Ala Lys Gly Ala Asp 
        35                   40                  45            
Gly Val Val Val Tyr Gln Gln Leu Asp Tyr Thr Pro Glu Thr Leu Gln 
    50                   55                  60                
Ala Leu Ala Asp Ala Gly Val Thr Lys Met Ser Leu Arg Asn Val Gly 
65                   70                  75                  80
Val Asp Asn Ile Asp Met Asp Lys Ala Lys Glu Leu Gly Phe Glu Ile 
                85                   90                  95    
Thr Asn Val Pro Val Tyr Ser Pro Asp Ala Ile Ala Glu His Ala Ala 
            100                  105                110        
Ile Gln Ala Ala Arg Val Leu Arg Gln Asp Lys Arg Met Asp Glu Lys 
        115                  120                125            
Met Ala Lys Arg Asp Leu Arg Trp Ala Pro Thr Ile Gly Arg Glu Val 
    130                  135                140                
Arg Asp Gln Val Val Gly Val Val Gly Thr Gly His Ile Gly Gln Val 
145                  150                155                  160
Phe Met Lys Ile Met Glu Gly Phe Gly Ala Lys Val Ile Ala Tyr Asp 
                165                  170                175    
Ile Phe Lys Asn Pro Glu Leu Glu Lys Lys Gly Tyr Tyr Val Asp Ser 
            180                  185                190        
Leu Asp Asp Leu Tyr Lys Gln Ala Asp Val Ile Ser Leu His Val Pro 
        195                  200                205            
Asp Val Pro Ala Asn Val His Met Ile Asn Asp Glu Ser Ile Ala Lys 
    210                  215                220                
Met Lys Asp Gly Val Val Ile Val Asn Cys Ser Arg Gly Pro Leu Val 
225                  230                235                  240
Asp Thr Asp Ala Val Ile Arg Gly Leu Asp Ser Gly Lys Ile Phe Gly 
                245                  250                255    
Phe Val Met Asp Thr Tyr Glu Gly Glu Val Gly Val Phe Asn Glu Asp 
            260                  265                270        
Trp Glu Gly Lys Glu Phe Pro Asp Ala Arg Leu Ala Asp Leu Ile Asp 
        275                  280                285            
Arg Pro Asn Val Leu Val Thr Pro His Thr Ala Phe Tyr Thr Thr His 
    290                  295                300                
Ala Val Arg Asn Met Val Thr Lys Ala Phe Asp Asn Asn Leu Lys Met 
305                  310                315                  320
Ile Asn Gly Glu Lys Pro Asp Ser Pro Val Ala Leu Asp Lys Asn Lys 
                325                  330                335    
Phe 
    

<210> 3
<211> 20
<212> DNA
<213> Lactobacillus johnsonii

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /organism="Lactobacillus johnsonii"

<400> 3
tcagcacata accagcagct                                                20


<210> 4
<211> 20
<212> DNA
<213> Lactobacillus johnsonii

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /organism="Lactobacillus johnsonii"

<400> 4
gcaataatac tgtcgccggt                                                20


<210> 5
<211> 20
<212> DNA
<213> Lactobacillus johnsonii

<220> 
<221> source
<222> 1..20
<223> /mol_type="DNA"
      /organism="Lactobacillus johnsonii"

<400> 5
gtgtataata aaagacggtc                                                20


<210> 6
<211> 1014
<212> DNA
<213> Lactobacillus johnsonii

<220> 
<221> source
<222> 1..1014
<223> /mol_type="DNA"
      /organism="Lactobacillus johnsonii"

<400> 6
atgacaaaga tttttgctta cgctattcgt aaagatgaag aacctttctt aaacgaatgg      60

aaagatgcac acaaggatat tgaagttgaa tacactgaca agcttttagc ccctgaaact     120

gctaaattag ctaagggtgc tgacggtgtt gttgtttacc aacaattaga ctacactcct     180

gaaactcttc aagctttagc tgatgctggc gtaactaaga tgtcattacg taacgttggt     240

gttgacaaca tcgacatgga caaggctaaa gaattaggct ttgaaatcac taacgttcct     300

gtatactctc ctgacgcaat tgctgaacat gctgcaattc aagctgctcg cgtactacgt     360

caagataagc gtatggatga aaagatggct aagcgcgact tacgctgggc acctactatt     420

ggtcgtgaag ttcgtgacca agttgttggt gttgtaggta ctggtcacat cggtcaagta     480

tttatgaaga ttatggaagg ctttggcgca aaagttattg cttacgatat cttcaagaac     540

ccagaacttg aaaagaaggg ttactacgtt gactcacttg atgacttata caagcaagct     600

gatgtaattt cacttcacgt tccagatgtt ccagcaaacg ttcacatgat taatgatgaa     660

tcaatcgcta agatgaaaga tggcgttgta atcgtaaact gctcacgtgg tccacttgtt     720

gacactgacg ctgttatccg cggcttagat tctggtaaga tctttggttt cgtaatgaac     780

acttacgaag gtgaagttgg tgtatttaac gaagattggg aaggtaaaga attcccagat     840

gctcgtttag ctgacttaat cgatcgtcca aatgtattgg taactccaca tactgctttc     900

tacactactc atgctgtacg taacatggta actaaagcat ttgacaacaa cttaaagatg     960

atcaacggtg aaaaaccaga ttctccagtt gctttggaca agaacaagtt ctaa          1014


<210> 7
<211> 337
<212> PRT
<213> Lactobacillus johnsonii

<220> 
<221> SOURCE
<222> 1..337
<223> /mol_type="protein"
      /organism="Lactobacillus johnsonii"

<400> 7
Met Thr Lys Ile Phe Ala Tyr Ala Ile Arg Lys Asp Glu Glu Pro Phe 
1               5                   10                   15    
Leu Asn Glu Trp Lys Asp Ala His Lys Asp Ile Glu Val Glu Tyr Thr 
            20                   25                  30        
Asp Lys Leu Leu Ala Pro Glu Thr Ala Lys Leu Ala Lys Gly Ala Asp 
        35                   40                  45            
Gly Val Val Val Tyr Gln Gln Leu Asp Tyr Thr Pro Glu Thr Leu Gln 
    50                   55                  60                
Ala Leu Ala Asp Ala Gly Val Thr Lys Met Ser Leu Arg Asn Val Gly 
65                   70                  75                  80
Val Asp Asn Ile Asp Met Asp Lys Ala Lys Glu Leu Gly Phe Glu Ile 
                85                   90                  95    
Thr Asn Val Pro Val Tyr Ser Pro Asp Ala Ile Ala Glu His Ala Ala 
            100                  105                110        
Ile Gln Ala Ala Arg Val Leu Arg Gln Asp Lys Arg Met Asp Glu Lys 
        115                  120                125            
Met Ala Lys Arg Asp Leu Arg Trp Ala Pro Thr Ile Gly Arg Glu Val 
    130                  135                140                
Arg Asp Gln Val Val Gly Val Val Gly Thr Gly His Ile Gly Gln Val 
145                  150                155                  160
Phe Met Lys Ile Met Glu Gly Phe Gly Ala Lys Val Ile Ala Tyr Asp 
                165                  170                175    
Ile Phe Lys Asn Pro Glu Leu Glu Lys Lys Gly Tyr Tyr Val Asp Ser 
            180                  185                190        
Leu Asp Asp Leu Tyr Lys Gln Ala Asp Val Ile Ser Leu His Val Pro 
        195                  200                205            
Asp Val Pro Ala Asn Val His Met Ile Asn Asp Glu Ser Ile Ala Lys 
    210                  215                220                
Met Lys Asp Gly Val Val Ile Val Asn Cys Ser Arg Gly Pro Leu Val 
225                  230                235                  240
Asp Thr Asp Ala Val Ile Arg Gly Leu Asp Ser Gly Lys Ile Phe Gly 
                245                  250                255    
Phe Val Met Asn Thr Tyr Glu Gly Glu Val Gly Val Phe Asn Glu Asp 
            260                  265                270        
Trp Glu Gly Lys Glu Phe Pro Asp Ala Arg Leu Ala Asp Leu Ile Asp 
        275                  280                285            
Arg Pro Asn Val Leu Val Thr Pro His Thr Ala Phe Tyr Thr Thr His 
    290                  295                300                
Ala Val Arg Asn Met Val Thr Lys Ala Phe Asp Asn Asn Leu Lys Met 
305                  310                315                  320
Ile Asn Gly Glu Lys Pro Asp Ser Pro Val Ala Leu Asp Lys Asn Lys 
                325                  330                335    
Phe 
    

