                         SEQUENCE LISTING

<110>  University of South Florida
       Mohapatra, Shyam S.
       Wang, Jia-Wang S.
 
<120>  Materials and Methods For Profiling MicroRNAs

<130>  USF.291C2XPCT

<150>  US 61/454,334
<151>  2011-03-18

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  47
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  UNIVERSAL PRIMER SEQUENCE FOR REVERSE TRANSRIPTION OF MIRNAS


<220>
<221>  misc_feature
<222>  (46)..(46)
<223>  n=a, g or c

<220>
<221>  misc_feature
<222>  (47)..(47)
<223>  n=a, g, c or t

<400>  1
tggctagtta agctcaccag ctcggtacca agcttaacta gccatnn                     47


<210>  2
<211>  24
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A UNIVERSAL REVERSE PRIMER SEQUENCE IN QPCR REACTION

<400>  2
tggctagtta agctcaccag ctcg                                              24


<210>  3
<211>  26
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A UNIVERSAL PROBE SEQUENCE

<400>  3
tggctagtta agcttggtac cgagct                                            26


<210>  4
<211>  22
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A FORWARD PRIMER SEQUENCE FOR QPCR REACTION

<400>  4
tgaggtagta ggttgtatag tt                                                22


<210>  5
<211>  22
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A FORWARD PRIMER SEQUENCE FOR QPCR REACTION

<400>  5
tagcttatca gactgatgtt ga                                                22


<210>  6
<211>  23
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A FORWARD PRIMER SEQUENCE FOR QPCR REACTION

<400>  6
tgtagtgttt cctactttat gga                                               23


<210>  7
<211>  22
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A FORWARD PRIMER SEQUENCE FOR QPCR REACTION

<400>  7
tctcccaacc cttgtaccag tg                                                22


<210>  8
<211>  24
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A FORWARD PRIMER SEQUENCE FOR QPCR REACTION

<400>  8
tgaaacatac acgggaaacc tctt                                              24


<210>  9
<211>  22
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A FORWARD PRIMER SEQUENCE FOR QPCR REACTION

<400>  9
aaaggctagg ctcacaacca aa                                                22


<210>  10
<211>  148
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A UNIVERSAL PRIMER SEQUENCE FOR REVERSE TRANSCRIPTION OF MIRNAS


<220>
<221>  misc_feature
<222>  (147)..(147)
<223>  n=a, g or c

<220>
<221>  misc_feature
<222>  (148)..(148)
<223>  n=a, g, c or t

<400>  10
ccatcaatcg tgtgtctcta tggatgctgt cacaacgaca tgtcagcctc tgactccagg       60

atctgtagac gctagctgac atgtcgttgt gacagcatcc atagagacac acgattgatg      120

gttttttttt tttttttttt ttttttnn                                         148


<210>  11
<211>  25
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A UNIVERSAL REVERSE PRIMER SEQUENCE IN QPCR REACTION

<400>  11
gcctctgact ccaggatctg tagac                                             25


<210>  12
<211>  46
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A UBLOCKER SEQUENCE USEFUL ACCORDING TO THE PRESENT INVENTION

<400>  12
cgacauguca gcuaggucgu ttcgguttca cuaucgcuac gcacag                      46


<210>  13
<211>  56
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A UNIVERSAL PRIMER SEQUENCE FOR REVERSE TRANSCRIPTION OF MIRNAS


<220>
<221>  misc_feature
<222>  (55)..(55)
<223>  n=a, g or c

<220>
<221>  misc_feature
<222>  (56)..(56)
<223>  n=a, g, c or t

<400>  13
cugugcguag cgauagugaa accgaaacga ccuagcugac augucgtttt ttttnn           56


<210>  14
<211>  28
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A UNIVERSAL REVERSE PRIMER SEQUENCE IN QPCR REACTION

<400>  14
ctgtgcgtag cgatagtgaa accgaaac                                          28


<210>  15
<211>  25
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A UNIVERSAL REVERSE PRIMER SEQUENCE IN QPCR REACTION

<400>  15
cgctgtactc caggatctgt agacg                                             25


<210>  16
<211>  15
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A UNIVERSAL HYDROLYSIS LNA PROBE SEQUENCE

<400>  16
accatcaatc gtgtg                                                        15


<210>  17
<211>  15
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A UNIVERSAL HYDROLYSIS LNA PROBE SEQUENCE

<400>  17
ctatggatgc tgtca                                                        15


<210>  18
<211>  15
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A UNIVERSAL HYDROLYSIS LNA PROBE SEQUENCE

<400>  18
cgacatgtca gctag                                                        15


<210>  19
<211>  39
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A PRIMER SEQUENCE DESIGNED FOR THE MMU-LET-7A MIRNA

<400>  19
cgcattgtta tgcctgaggt agtaggttgt atagttaaa                              39


<210>  20
<211>  32
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A PRIMER SEQUENCE DESIGNED FOR THE MMU-LET-7C MIRNA

<400>  20
agcccagtga ggtagtaggt tgtatggtta aa                                     32


<210>  21
<211>  34
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A PRIMER SEQUENCE DESIGNED FOR TEH MMU-LET-7B MIRNA

<400>  21
ctcctgattt gaggtagtag gttgtgtggt taaa                                   34


<210>  22
<211>  36
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A PRIMER SEQUENCE DESIGNED FOR THE MMU-LET-7D MIRNA

<400>  22
ccgagagatg aagaggtagt aggttgcata gttaaa                                 36


<210>  23
<211>  39
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A PRIMER SEQUENCE DESIGNED FOR THE MMU-LET-7E MIRNA

<400>  23
gcttgaccag attatgaggt aggaggttgt atagttaaa                              39


<210>  24
<211>  43
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A PRIMER SEQUENCE DESIGNED FOR THE MMU-LET-7F MIRNA

<400>  24
gcacattgga cgatgatttg aggtagtaga ttgtatagtt aaa                         43


<210>  25
<211>  35
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A PRIMER SEQUENCE DESIGNED FOR THE MMU-LET-7G MIRNA

<400>  25
actccaccga tgaggtagta gtttgtacag ttaaa                                  35


<210>  26
<211>  35
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  A PRIMER SEQUENCE DESIGNED FOR THE MMU-LET-7I MIRNA

<400>  26
cagtggttac tgaggtagta gtttgtgctg ttaaa                                  35


<210>  27
<211>  31
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  POLY(A) SEQUENCE


<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  n=a, g, c or t

<400>  27
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa n                                      31


<210>  28
<211>  26
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  POLY(T) SEQUENCE


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n=a, g or c

<400>  28
nttttttttt tttttttttt tttttt                                            26


<210>  29
<211>  28
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  POLY(A) SEQUENCE


<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  n=a, g, c or t

<400>  29
aaaaaaaaaa aaaaaaaaaa aaaaaaan                                          28


<210>  30
<211>  25
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  POLY(A) SEQUENCE

<400>  30
tttttttttt tttttttttt ttttt                                             25


