                         SEQUENCE LISTING

<110>  Commissariat  l'Energie Atomique et aux Energies 
       Alternatives (CEA)
 
<120>  PEPTIDES APTES A FORMER UN COMPLEXE COVALENT ET LEURS 
       UTILISATIONS

<130>  SP39394 (PCT)/SG

<140>  PCT/EP2012/XXXXXX
<141>  2012-03-26

<150>  FR11/52432
<151>  2011-03-24

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  225
<212>  DNA
<213>  Streptococcus pneumoniae


<220>
<221>  CDS
<222>  (1)..(225)
<223>  Squence codant le peptide Jo

<400>  1
cag tat cca caa aca ggg act tat cca gat gtt caa aca cct tat cag         48
Gln Tyr Pro Gln Thr Gly Thr Tyr Pro Asp Val Gln Thr Pro Tyr Gln           
1               5                   10                  15                

att att aag gta gat ggt tcg gaa aaa aac gga cag cac aag gcg ttg         96
Ile Ile Lys Val Asp Gly Ser Glu Lys Asn Gly Gln His Lys Ala Leu           
            20                  25                  30                    

aat ccg aat cca tat gaa cgt gtg att cca gaa ggt aca ctt tca aag        144
Asn Pro Asn Pro Tyr Glu Arg Val Ile Pro Glu Gly Thr Leu Ser Lys           
        35                  40                  45                        

aga att tat caa gtg aat aat ttg gat gat aac caa tat gga atc gaa        192
Arg Ile Tyr Gln Val Asn Asn Leu Asp Asp Asn Gln Tyr Gly Ile Glu           
    50                  55                  60                            

ttg acg gtt agt ggg aaa aca gtg tat gaa caa                            225
Leu Thr Val Ser Gly Lys Thr Val Tyr Glu Gln                               
65                  70                  75                                


<210>  2
<211>  75
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  2

Gln Tyr Pro Gln Thr Gly Thr Tyr Pro Asp Val Gln Thr Pro Tyr Gln 
1               5                   10                  15      


Ile Ile Lys Val Asp Gly Ser Glu Lys Asn Gly Gln His Lys Ala Leu 
            20                  25                  30          


Asn Pro Asn Pro Tyr Glu Arg Val Ile Pro Glu Gly Thr Leu Ser Lys 
        35                  40                  45              


Arg Ile Tyr Gln Val Asn Asn Leu Asp Asp Asn Gln Tyr Gly Ile Glu 
    50                  55                  60                  


Leu Thr Val Ser Gly Lys Thr Val Tyr Glu Gln 
65                  70                  75  


<210>  3
<211>  390
<212>  DNA
<213>  Streptococcus pneumoniae


<220>
<221>  CDS
<222>  (1)..(384)
<223>  Squence codant le peptide In

<400>  3
att gag aat ggt acg att aca gat ccg atg ggt gag tta att gat ttg         48
Ile Glu Asn Gly Thr Ile Thr Asp Pro Met Gly Glu Leu Ile Asp Leu           
1               5                   10                  15                

caa ttg ggc aca gat gga aga ttt gat cca gca gat tac act tta act         96
Gln Leu Gly Thr Asp Gly Arg Phe Asp Pro Ala Asp Tyr Thr Leu Thr           
            20                  25                  30                    

gca aac gat ggt agt cgc ttg gag aat gga caa gct gta ggt ggt cca        144
Ala Asn Asp Gly Ser Arg Leu Glu Asn Gly Gln Ala Val Gly Gly Pro           
        35                  40                  45                        

caa aat gat ggt ggt ttg tta aaa aat gca aaa gtg ctc tat gat acg        192
Gln Asn Asp Gly Gly Leu Leu Lys Asn Ala Lys Val Leu Tyr Asp Thr           
    50                  55                  60                            

act gag aaa agg att cgt gta aca ggt ctg tac ctt gga acg gat gaa        240
Thr Glu Lys Arg Ile Arg Val Thr Gly Leu Tyr Leu Gly Thr Asp Glu           
65                  70                  75                  80            

aaa gtt acg ttg acc tac aat gtt cgt ttg aat gat gag ttt gta agc        288
Lys Val Thr Leu Thr Tyr Asn Val Arg Leu Asn Asp Glu Phe Val Ser           
                85                  90                  95                

aat aaa ttt tat gat acc aat ggt cga aca acc tta cat cct aag gaa        336
Asn Lys Phe Tyr Asp Thr Asn Gly Arg Thr Thr Leu His Pro Lys Glu           
            100                 105                 110                   

gta gaa cag aac aca gtg cgc gac ttc ccg att cct aag att cgt gat        384
Val Glu Gln Asn Thr Val Arg Asp Phe Pro Ile Pro Lys Ile Arg Asp           
        115                 120                 125                       

cgtgat                                                                 390


<210>  4
<211>  128
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  4

Ile Glu Asn Gly Thr Ile Thr Asp Pro Met Gly Glu Leu Ile Asp Leu 
1               5                   10                  15      


Gln Leu Gly Thr Asp Gly Arg Phe Asp Pro Ala Asp Tyr Thr Leu Thr 
            20                  25                  30          


Ala Asn Asp Gly Ser Arg Leu Glu Asn Gly Gln Ala Val Gly Gly Pro 
        35                  40                  45              


Gln Asn Asp Gly Gly Leu Leu Lys Asn Ala Lys Val Leu Tyr Asp Thr 
    50                  55                  60                  


Thr Glu Lys Arg Ile Arg Val Thr Gly Leu Tyr Leu Gly Thr Asp Glu 
65                  70                  75                  80  


Lys Val Thr Leu Thr Tyr Asn Val Arg Leu Asn Asp Glu Phe Val Ser 
                85                  90                  95      


Asn Lys Phe Tyr Asp Thr Asn Gly Arg Thr Thr Leu His Pro Lys Glu 
            100                 105                 110         


Val Glu Gln Asn Thr Val Arg Asp Phe Pro Ile Pro Lys Ile Arg Asp 
        115                 120                 125             


<210>  5
<211>  237
<212>  DNA
<213>  Unknown

<220>
<223>  Artificial sequence


<220>
<221>  CDS
<222>  (1)..(237)
<223>  Squence codant un driv du peptide Jo

<400>  5
tct gac cag tat cca caa aca ggg act tat cca gat gtt caa aca cct         48
Ser Asp Gln Tyr Pro Gln Thr Gly Thr Tyr Pro Asp Val Gln Thr Pro           
1               5                   10                  15                

tat cag att att aag gta gat ggt tcg gaa aaa aac gga cag cac aag         96
Tyr Gln Ile Ile Lys Val Asp Gly Ser Glu Lys Asn Gly Gln His Lys           
            20                  25                  30                    

gcg ttg aat ccg aat cca tat gaa cgt gtg att cca gaa ggt aca ctt        144
Ala Leu Asn Pro Asn Pro Tyr Glu Arg Val Ile Pro Glu Gly Thr Leu           
        35                  40                  45                        

tca aag aga att tat caa gtg aat aat ttg gat gat aac caa tat gga        192
Ser Lys Arg Ile Tyr Gln Val Asn Asn Leu Asp Asp Asn Gln Tyr Gly           
    50                  55                  60                            

atc gaa ttg acg gtt agt ggg aaa aca gtg tat gaa caa aaa gat            237
Ile Glu Leu Thr Val Ser Gly Lys Thr Val Tyr Glu Gln Lys Asp               
65                  70                  75                                


<210>  6
<211>  79
<212>  PRT
<213>  Unknown

<220>
<223>  Synthetic Construct

<400>  6

Ser Asp Gln Tyr Pro Gln Thr Gly Thr Tyr Pro Asp Val Gln Thr Pro 
1               5                   10                  15      


Tyr Gln Ile Ile Lys Val Asp Gly Ser Glu Lys Asn Gly Gln His Lys 
            20                  25                  30          


Ala Leu Asn Pro Asn Pro Tyr Glu Arg Val Ile Pro Glu Gly Thr Leu 
        35                  40                  45              


Ser Lys Arg Ile Tyr Gln Val Asn Asn Leu Asp Asp Asn Gln Tyr Gly 
    50                  55                  60                  


Ile Glu Leu Thr Val Ser Gly Lys Thr Val Tyr Glu Gln Lys Asp 
65                  70                  75                  


<210>  7
<211>  399
<212>  DNA
<213>  Unknown

<220>
<223>  Artificial sequence


<220>
<221>  CDS
<222>  (1)..(399)
<223>  Squence codant un driv du peptide In

<400>  7
act gaa aag aaa tca att gag aat ggt acg att aca gat ccg atg ggt         48
Thr Glu Lys Lys Ser Ile Glu Asn Gly Thr Ile Thr Asp Pro Met Gly           
1               5                   10                  15                

gag tta att gat ttg caa ttg ggc aca gat gga aga ttt gat cca gca         96
Glu Leu Ile Asp Leu Gln Leu Gly Thr Asp Gly Arg Phe Asp Pro Ala           
            20                  25                  30                    

gat tac act tta act gca aac gat ggt agt cgc ttg gag aat gga caa        144
Asp Tyr Thr Leu Thr Ala Asn Asp Gly Ser Arg Leu Glu Asn Gly Gln           
        35                  40                  45                        

gct gta ggt ggt cca caa aat gat ggt ggt ttg tta aaa aat gca aaa        192
Ala Val Gly Gly Pro Gln Asn Asp Gly Gly Leu Leu Lys Asn Ala Lys           
    50                  55                  60                            

gtg ctc tat gat acg act gag aaa agg att cgt gta aca ggt ctg tac        240
Val Leu Tyr Asp Thr Thr Glu Lys Arg Ile Arg Val Thr Gly Leu Tyr           
65                  70                  75                  80            

ctt gga acg gat gaa aaa gtt acg ttg acc tac aat gtt cgt ttg aat        288
Leu Gly Thr Asp Glu Lys Val Thr Leu Thr Tyr Asn Val Arg Leu Asn           
                85                  90                  95                

gat gag ttt gta agc aat aaa ttt tat gat acc aat ggt cga aca acc        336
Asp Glu Phe Val Ser Asn Lys Phe Tyr Asp Thr Asn Gly Arg Thr Thr           
            100                 105                 110                   

tta cat cct aag gaa gta gaa cag aac aca gtg cgc gac ttc ccg att        384
Leu His Pro Lys Glu Val Glu Gln Asn Thr Val Arg Asp Phe Pro Ile           
        115                 120                 125                       

cct aag att cgt gat                                                    399
Pro Lys Ile Arg Asp                                                       
    130                                                                   


<210>  8
<211>  133
<212>  PRT
<213>  Unknown

<220>
<223>  Synthetic Construct

<400>  8

Thr Glu Lys Lys Ser Ile Glu Asn Gly Thr Ile Thr Asp Pro Met Gly 
1               5                   10                  15      


Glu Leu Ile Asp Leu Gln Leu Gly Thr Asp Gly Arg Phe Asp Pro Ala 
            20                  25                  30          


Asp Tyr Thr Leu Thr Ala Asn Asp Gly Ser Arg Leu Glu Asn Gly Gln 
        35                  40                  45              


Ala Val Gly Gly Pro Gln Asn Asp Gly Gly Leu Leu Lys Asn Ala Lys 
    50                  55                  60                  


Val Leu Tyr Asp Thr Thr Glu Lys Arg Ile Arg Val Thr Gly Leu Tyr 
65                  70                  75                  80  


Leu Gly Thr Asp Glu Lys Val Thr Leu Thr Tyr Asn Val Arg Leu Asn 
                85                  90                  95      


Asp Glu Phe Val Ser Asn Lys Phe Tyr Asp Thr Asn Gly Arg Thr Thr 
            100                 105                 110         


Leu His Pro Lys Glu Val Glu Gln Asn Thr Val Arg Asp Phe Pro Ile 
        115                 120                 125             


Pro Lys Ile Arg Asp 
    130             


<210>  9
<211>  92
<212>  PRT
<213>  Unknown

<220>
<223>  Artificial sequence


<220>
<221>  PEPTIDE
<222>  (1)..(92)
<223>  Peptide HisJo

<400>  9

Gly Ser Ser His His His His His His Ser Gln Asp Pro Ser Asp Gln 
1               5                   10                  15      


Tyr Pro Gln Thr Gly Thr Tyr Pro Asp Val Gln Thr Pro Tyr Gln Ile 
            20                  25                  30          


Ile Lys Val Asp Gly Ser Glu Lys Asn Gly Gln His Lys Ala Leu Asn 
        35                  40                  45              


Pro Asn Pro Tyr Glu Arg Val Ile Pro Glu Gly Thr Leu Ser Lys Arg 
    50                  55                  60                  


Ile Tyr Gln Val Asn Asn Leu Asp Asp Asn Gln Tyr Gly Ile Glu Leu 
65                  70                  75                  80  


Thr Val Ser Gly Lys Thr Val Tyr Glu Gln Lys Asp 
                85                  90          


<210>  10
<211>  146
<212>  PRT
<213>  Unknown

<220>
<223>  Artificial sequence


<220>
<221>  PEPTIDE
<222>  (1)..(146)
<223>  Peptide HisIn

<400>  10

Gly Ser Ser His His His His His His Ser Gln Asp Pro Thr Glu Lys 
1               5                   10                  15      


Lys Ser Ile Glu Asn Gly Thr Ile Thr Asp Pro Met Gly Glu Leu Ile 
            20                  25                  30          


Asp Leu Gln Leu Gly Thr Asp Gly Arg Phe Asp Pro Ala Asp Tyr Thr 
        35                  40                  45              


Leu Thr Ala Asn Asp Gly Ser Arg Leu Glu Asn Gly Gln Ala Val Gly 
    50                  55                  60                  


Gly Pro Gln Asn Asp Gly Gly Leu Leu Lys Asn Ala Lys Val Leu Tyr 
65                  70                  75                  80  


Asp Thr Thr Glu Lys Arg Ile Arg Val Thr Gly Leu Tyr Leu Gly Thr 
                85                  90                  95      


Asp Glu Lys Val Thr Leu Thr Tyr Asn Val Arg Leu Asn Asp Glu Phe 
            100                 105                 110         


Val Ser Asn Lys Phe Tyr Asp Thr Asn Gly Arg Thr Thr Leu His Pro 
        115                 120                 125             


Lys Glu Val Glu Gln Asn Thr Val Arg Asp Phe Pro Ile Pro Lys Ile 
    130                 135                 140                 


Arg Asp 
145     


<210>  11
<211>  228
<212>  PRT
<213>  Unknown

<220>
<223>  Artificial sequence


<220>
<221>  PEPTIDE
<222>  (1)..(228)
<223>  Peptide HisJoIn

<400>  11

Gly Ser Ser His His His His His His Ser Gln Asp Pro Ser Asp Gln 
1               5                   10                  15      


Tyr Pro Gln Thr Gly Thr Tyr Pro Asp Val Gln Thr Pro Tyr Gln Ile 
            20                  25                  30          


Ile Lys Val Asp Gly Ser Glu Lys Asn Gly Gln His Lys Ala Leu Asn 
        35                  40                  45              


Pro Asn Pro Tyr Glu Arg Val Ile Pro Glu Gly Thr Leu Ser Lys Arg 
    50                  55                  60                  


Ile Tyr Gln Val Asn Asn Leu Asp Asp Asn Gln Tyr Gly Ile Glu Leu 
65                  70                  75                  80  


Thr Val Ser Gly Lys Thr Val Tyr Glu Gln Lys Asp Ala Asp Leu Thr 
                85                  90                  95      


Glu Lys Lys Ser Ile Glu Asn Gly Thr Ile Thr Asp Pro Met Gly Glu 
            100                 105                 110         


Leu Ile Asp Leu Gln Leu Gly Thr Asp Gly Arg Phe Asp Pro Ala Asp 
        115                 120                 125             


Tyr Thr Leu Thr Ala Asn Asp Gly Ser Arg Leu Glu Asn Gly Gln Ala 
    130                 135                 140                 


Val Gly Gly Pro Gln Asn Asp Gly Gly Leu Leu Lys Asn Ala Lys Val 
145                 150                 155                 160 


Leu Tyr Asp Thr Thr Glu Lys Arg Ile Arg Val Thr Gly Leu Tyr Leu 
                165                 170                 175     


Gly Thr Asp Glu Lys Val Thr Leu Thr Tyr Asn Val Arg Leu Asn Asp 
            180                 185                 190         


Glu Phe Val Ser Asn Lys Phe Tyr Asp Thr Asn Gly Arg Thr Thr Leu 
        195                 200                 205             


His Pro Lys Glu Val Glu Gln Asn Thr Val Arg Asp Phe Pro Ile Pro 
    210                 215                 220                 


Lys Ile Arg Asp 
225             


<210>  12
<211>  239
<212>  PRT
<213>  Unknown

<220>
<223>  Artificial sequence


<220>
<221>  PEPTIDE
<222>  (1)..(239)
<223>  Peptide HisJoHisIn

<400>  12

Gly Ser Ser His His His His His His Ser Gln Asp Pro Ser Asp Gln 
1               5                   10                  15      


Tyr Pro Gln Thr Gly Thr Tyr Pro Asp Val Gln Thr Pro Tyr Gln Ile 
            20                  25                  30          


Ile Lys Val Asp Gly Ser Glu Lys Asn Gly Gln His Lys Ala Leu Asn 
        35                  40                  45              


Pro Asn Pro Tyr Glu Arg Val Ile Pro Glu Gly Thr Leu Ser Lys Arg 
    50                  55                  60                  


Ile Tyr Gln Val Asn Asn Leu Asp Asp Asn Gln Tyr Gly Ile Glu Leu 
65                  70                  75                  80  


Thr Val Ser Gly Lys Thr Val Tyr Glu Gln Lys Asp Met Gly Ser Ser 
                85                  90                  95      


His His His His His His Ser Gln Asp Pro Thr Glu Lys Lys Ser Ile 
            100                 105                 110         


Glu Asn Gly Thr Ile Thr Asp Pro Met Gly Glu Leu Ile Asp Leu Gln 
        115                 120                 125             


Leu Gly Thr Asp Gly Arg Phe Asp Pro Ala Asp Tyr Thr Leu Thr Ala 
    130                 135                 140                 


Asn Asp Gly Ser Arg Leu Glu Asn Gly Gln Ala Val Gly Gly Pro Gln 
145                 150                 155                 160 


Asn Asp Gly Gly Leu Leu Lys Asn Ala Lys Val Leu Tyr Asp Thr Thr 
                165                 170                 175     


Glu Lys Arg Ile Arg Val Thr Gly Leu Tyr Leu Gly Thr Asp Glu Lys 
            180                 185                 190         


Val Thr Leu Thr Tyr Asn Val Arg Leu Asn Asp Glu Phe Val Ser Asn 
        195                 200                 205             


Lys Phe Tyr Asp Thr Asn Gly Arg Thr Thr Leu His Pro Lys Glu Val 
    210                 215                 220                 


Glu Gln Asn Thr Val Arg Asp Phe Pro Ile Pro Lys Ile Arg Asp 
225                 230                 235                 


<210>  13
<211>  232
<212>  PRT
<213>  Unknown

<220>
<223>  Artificial sequence


<220>
<221>  PEPTIDE
<222>  (1)..(232)
<223>  Peptide FusJoIn

<400>  13

Gly Ser Ser His His His His His His Ser Gln Asp Pro Ser Asp Gln 
1               5                   10                  15      


Tyr Pro Gln Thr Gly Thr Tyr Pro Asp Val Gln Thr Pro Tyr Gln Ile 
            20                  25                  30          


Ile Lys Val Asp Gly Ser Glu Lys Asn Gly Gln His Lys Ala Leu Asn 
        35                  40                  45              


Pro Asn Pro Tyr Glu Arg Val Ile Pro Glu Gly Thr Leu Ser Lys Arg 
    50                  55                  60                  


Ile Tyr Gln Val Asn Asn Leu Asp Asp Asn Gln Tyr Gly Ile Glu Leu 
65                  70                  75                  80  


Thr Val Ser Gly Lys Thr Val Tyr Glu Gln Lys Asp Gly Ser Thr Pro 
                85                  90                  95      


Gly Ser Val Thr Glu Lys Lys Ser Ile Glu Asn Gly Thr Ile Thr Asp 
            100                 105                 110         


Pro Met Gly Glu Leu Ile Asp Leu Gln Leu Gly Thr Asp Gly Arg Phe 
        115                 120                 125             


Asp Pro Ala Asp Tyr Thr Leu Thr Ala Asn Asp Gly Ser Arg Leu Glu 
    130                 135                 140                 


Asn Gly Gln Ala Val Gly Gly Pro Gln Asn Asp Gly Gly Leu Leu Lys 
145                 150                 155                 160 


Asn Ala Lys Val Leu Tyr Asp Thr Thr Glu Lys Arg Ile Arg Val Thr 
                165                 170                 175     


Gly Leu Tyr Leu Gly Thr Asp Glu Lys Val Thr Leu Thr Tyr Asn Val 
            180                 185                 190         


Arg Leu Asn Asp Glu Phe Val Ser Asn Lys Phe Tyr Asp Thr Asn Gly 
        195                 200                 205             


Arg Thr Thr Leu His Pro Lys Glu Val Glu Gln Asn Thr Val Arg Asp 
    210                 215                 220                 


Phe Pro Ile Pro Lys Ile Arg Asp 
225                 230         


<210>  14
<211>  86
<212>  DNA
<213>  Unknown

<220>
<223>  Squence dans le plasmide driv de pET-Duet-1


<220>
<221>  misc_feature
<222>  (1)..(35)
<223>  Squence partielle d'un driv de Jo

<220>
<221>  CDS
<222>  (36)..(56)
<223>  Squence codant un linker de 7 acides amins

<220>
<221>  misc_feature
<222>  (57)..(86)
<223>  squence partielle d'un driv de In

<400>  14
cggttagtgg gaaaacagtg tatgaacaaa aagat ggt tct acc ccg ggt tct          53
                                       Gly Ser Thr Pro Gly Ser            
                                       1               5                  

gtt actgaaaaga aatcaattga gaatggtacg                                    86
Val                                                                       
                                                                          


<210>  15
<211>  7
<212>  PRT
<213>  Unknown

<220>
<223>  Synthetic Construct

<400>  15

Gly Ser Thr Pro Gly Ser Val 
1               5           


<210>  16
<211>  98
<212>  DNA
<213>  Unknown

<220>
<223>  Oligomre 1

<400>  16
cggttagtgg gaaaacagtg tatgaacaaa aagatggaat tcggtacccc tgcagggagc       60

tgaagcttac tgaaaagaaa tcaattgaga atggtacg                               98


<210>  17
<211>  98
<212>  DNA
<213>  Unknown

<220>
<223>  Oligomre 2

<400>  17
cgtaccattc tcaattgatt tcttttcagt aagcttcagc tccctgcagg ggtaccgaat       60

tccatctttt tgttcataca ctgttttccc actaaccg                               98


<210>  18
<211>  30
<212>  DNA
<213>  Unknown

<220>
<223>  Oligomre 3

<400>  18
gcgcgaattc cagaaatttc tcagcgtacg                                        30


<210>  19
<211>  27
<212>  DNA
<213>  Unknown

<220>
<223>  Oligomre 4

<400>  19
gcgcaagctt gctgatttgg tttgatg                                           27


<210>  20
<211>  756
<212>  DNA
<213>  Unknown

<220>
<223>  Squence nuclotidique de la rgion pertinente du vecteur d 
       clonage permettant l'expression d'une protine de fusion ayant 
       des drivs de Jo et In respectivement en N- et C-terminus de la 
       protine


<220>
<221>  misc_feature
<222>  (43)..(60)
<223>  Squence nuclotidique codant pour 6 histidines

<220>
<221>  misc_feature
<222>  (73)..(309)
<223>  Squence nuclotidique codant pour un driv de Jo

<220>
<221>  misc_feature
<222>  (310)..(342)
<223>  Site de clonage multiple (5 sites de restriction)

<220>
<221>  misc_feature
<222>  (311)..(316)
<223>  Site de restriction EcoRI

<220>
<221>  misc_feature
<222>  (317)..(322)
<223>  Site de restriction KpnI

<220>
<221>  misc_feature
<222>  (324)..(329)
<223>  Site de restriction PstI

<220>
<221>  misc_feature
<222>  (331)..(336)
<223>  Site de restriction SacI

<220>
<221>  misc_feature
<222>  (337)..(342)
<223>  Site de restriction HindIII

<220>
<221>  misc_feature
<222>  (343)..(744)
<223>  Squence nuclotidique codant pour un driv de In

<400>  20
ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcacca tcatcaccac       60

agccaggatc cgtctgacca gtatccacaa acagggactt atccagatgt tcaaacacct      120

tatcagatta ttaaggtaga tggttcggaa aaaaacggac agcacaaggc gttgaatccg      180

aatccatatg aacgtgtgat tccagaaggt acactttcaa agagaattta tcaagtgaat      240

aatttggatg ataaccaata tggaatcgaa ttgacggtta gtgggaaaac agtgtatgaa      300

caaaaagatg gaattcggta cccctgcagg gagctgaagc ttactgaaaa gaaatcaatt      360

gagaatggta cgattacaga tccgatgggt gagttaattg atttgcaatt gggcacagat      420

ggaagatttg atccagcaga ttacacttta actgcaaacg atggtagtcg cttggagaat      480

ggacaagctg taggtggtcc acaaaatgat ggtggtttgt taaaaaatgc aaaagtgctc      540

tatgatacga ctgagaaaag gattcgtgta acaggtctgt accttggaac ggatgaaaaa      600

gttacgttga cctacaatgt tcgtttgaat gatgagtttg taagcaataa attttatgat      660

accaatggtc gaacaacctt acatcctaag gaagtagaac agaacacagt gcgcgacttc      720

ccgattccta agattcgtga ttgactcgag tctggt                                756


<210>  21
<211>  867
<212>  DNA
<213>  Unknown

<220>
<223>  Squence nuclotidique de la rgion pertinente du vecteur 
       permettant l'expression d'une protine de fusion ayant des 
       drivs de Jo et In localises respectivement en N et C-terminus 
       de la boucle 1 du canal potassique


<220>
<221>  CDS
<222>  (1)..(855)

<220>
<221>  misc_feature
<222>  (13)..(30)
<223>  Squence nuclotidique codant pour 6 histidines

<220>
<221>  misc_feature
<222>  (43)..(279)
<223>  Squence nuclotidique codant pour un driv de Jo

<220>
<221>  misc_feature
<222>  (280)..(288)
<223>  Squence nuclotidique codant pour des acides amins du linker

<220>
<221>  misc_feature
<222>  (289)..(447)
<223>  Squence nuclotidique codant pour la boucle 1 du canal 
       potassique

<220>
<221>  misc_feature
<222>  (448)..(453)
<223>  Squence nuclotidique codant pour des acides amins du linker

<220>
<221>  misc_feature
<222>  (454)..(855)
<223>  Squence nuclotidique codant pour un driv de In

<400>  21
atg ggc agc agc cat cac cat cat cac cac agc cag gat ccg tct gac         48
Met Gly Ser Ser His His His His His His Ser Gln Asp Pro Ser Asp           
1               5                   10                  15                

cag tat cca caa aca ggg act tat cca gat gtt caa aca cct tat cag         96
Gln Tyr Pro Gln Thr Gly Thr Tyr Pro Asp Val Gln Thr Pro Tyr Gln           
            20                  25                  30                    

att att aag gta gat ggt tcg gaa aaa aac gga cag cac aag gcg ttg        144
Ile Ile Lys Val Asp Gly Ser Glu Lys Asn Gly Gln His Lys Ala Leu           
        35                  40                  45                        

aat ccg aat cca tat gaa cgt gtg att cca gaa ggt aca ctt tca aag        192
Asn Pro Asn Pro Tyr Glu Arg Val Ile Pro Glu Gly Thr Leu Ser Lys           
    50                  55                  60                            

aga att tat caa gtg aat aat ttg gat gat aac caa tat gga atc gaa        240
Arg Ile Tyr Gln Val Asn Asn Leu Asp Asp Asn Gln Tyr Gly Ile Glu           
65                  70                  75                  80            

ttg acg gtt agt ggg aaa aca gtg tat gaa caa aaa gat gga att cca        288
Leu Thr Val Ser Gly Lys Thr Val Tyr Glu Gln Lys Asp Gly Ile Pro           
                85                  90                  95                

gaa att tct cag cgt acg acc att gtg att cag aaa caa acg ttt atc        336
Glu Ile Ser Gln Arg Thr Thr Ile Val Ile Gln Lys Gln Thr Phe Ile           
            100                 105                 110                   

agt cag cat tcc tgt gtg aat agc acc gaa ctg gat gaa ctg atc caa        384
Ser Gln His Ser Cys Val Asn Ser Thr Glu Leu Asp Glu Leu Ile Gln           
        115                 120                 125                       

caa att gtg gca gcg atc aat gcc ggt att att cca ctg ggg aac aca        432
Gln Ile Val Ala Ala Ile Asn Ala Gly Ile Ile Pro Leu Gly Asn Thr           
    130                 135                 140                           

tca aac caa atc agc aag ctt act gaa aag aaa tca att gag aat ggt        480
Ser Asn Gln Ile Ser Lys Leu Thr Glu Lys Lys Ser Ile Glu Asn Gly           
145                 150                 155                 160           

acg att aca gat ccg atg ggt gag tta att gat ttg caa ttg ggc aca        528
Thr Ile Thr Asp Pro Met Gly Glu Leu Ile Asp Leu Gln Leu Gly Thr           
                165                 170                 175               

gat gga aga ttt gat cca gca gat tac act tta act gca aac gat ggt        576
Asp Gly Arg Phe Asp Pro Ala Asp Tyr Thr Leu Thr Ala Asn Asp Gly           
            180                 185                 190                   

agt cgc ttg gag aat gga caa gct gta ggt ggt cca caa aat gat ggt        624
Ser Arg Leu Glu Asn Gly Gln Ala Val Gly Gly Pro Gln Asn Asp Gly           
        195                 200                 205                       

ggt ttg tta aaa aat gca aaa gtg ctc tat gat acg act gag aaa agg        672
Gly Leu Leu Lys Asn Ala Lys Val Leu Tyr Asp Thr Thr Glu Lys Arg           
    210                 215                 220                           

att cgt gta aca ggt ctg tac ctt gga acg gat gaa aaa gtt acg ttg        720
Ile Arg Val Thr Gly Leu Tyr Leu Gly Thr Asp Glu Lys Val Thr Leu           
225                 230                 235                 240           

acc tac aat gtt cgt ttg aat gat gag ttt gta agc aat aaa ttt tat        768
Thr Tyr Asn Val Arg Leu Asn Asp Glu Phe Val Ser Asn Lys Phe Tyr           
                245                 250                 255               

gat acc aat ggt cga aca acc tta cat cct aag gaa gta gaa cag aac        816
Asp Thr Asn Gly Arg Thr Thr Leu His Pro Lys Glu Val Glu Gln Asn           
            260                 265                 270                   

aca gtg cgc gac ttc ccg att cct aag att cgt gat tga ctcgagtctg gt      867
Thr Val Arg Asp Phe Pro Ile Pro Lys Ile Arg Asp                           
        275                 280                                           


<210>  22
<211>  284
<212>  PRT
<213>  Unknown

<220>
<223>  Synthetic Construct

<400>  22

Met Gly Ser Ser His His His His His His Ser Gln Asp Pro Ser Asp 
1               5                   10                  15      


Gln Tyr Pro Gln Thr Gly Thr Tyr Pro Asp Val Gln Thr Pro Tyr Gln 
            20                  25                  30          


Ile Ile Lys Val Asp Gly Ser Glu Lys Asn Gly Gln His Lys Ala Leu 
        35                  40                  45              


Asn Pro Asn Pro Tyr Glu Arg Val Ile Pro Glu Gly Thr Leu Ser Lys 
    50                  55                  60                  


Arg Ile Tyr Gln Val Asn Asn Leu Asp Asp Asn Gln Tyr Gly Ile Glu 
65                  70                  75                  80  


Leu Thr Val Ser Gly Lys Thr Val Tyr Glu Gln Lys Asp Gly Ile Pro 
                85                  90                  95      


Glu Ile Ser Gln Arg Thr Thr Ile Val Ile Gln Lys Gln Thr Phe Ile 
            100                 105                 110         


Ser Gln His Ser Cys Val Asn Ser Thr Glu Leu Asp Glu Leu Ile Gln 
        115                 120                 125             


Gln Ile Val Ala Ala Ile Asn Ala Gly Ile Ile Pro Leu Gly Asn Thr 
    130                 135                 140                 


Ser Asn Gln Ile Ser Lys Leu Thr Glu Lys Lys Ser Ile Glu Asn Gly 
145                 150                 155                 160 


Thr Ile Thr Asp Pro Met Gly Glu Leu Ile Asp Leu Gln Leu Gly Thr 
                165                 170                 175     


Asp Gly Arg Phe Asp Pro Ala Asp Tyr Thr Leu Thr Ala Asn Asp Gly 
            180                 185                 190         


Ser Arg Leu Glu Asn Gly Gln Ala Val Gly Gly Pro Gln Asn Asp Gly 
        195                 200                 205             


Gly Leu Leu Lys Asn Ala Lys Val Leu Tyr Asp Thr Thr Glu Lys Arg 
    210                 215                 220                 


Ile Arg Val Thr Gly Leu Tyr Leu Gly Thr Asp Glu Lys Val Thr Leu 
225                 230                 235                 240 


Thr Tyr Asn Val Arg Leu Asn Asp Glu Phe Val Ser Asn Lys Phe Tyr 
                245                 250                 255     


Asp Thr Asn Gly Arg Thr Thr Leu His Pro Lys Glu Val Glu Gln Asn 
            260                 265                 270         


Thr Val Arg Asp Phe Pro Ile Pro Lys Ile Arg Asp 
        275                 280                 


