                         SEQUENCE LISTING

<110>  Volker Sieber, Andr Pick, Broder Rhmann
 
<120>  Synthetic pathway for the production of chemicals with reduced 
       number of functional groups

<130>  155 415

<150>  EP 11159592
<151>  2011-03-24

<160>  6     

<170>  PatentIn version 3.4

<210>  1
<211>  265
<212>  PRT
<213>  Agrobacterium tumefaciens

<400>  1

Met Lys Arg Leu Leu Val Thr Gly Ala Ala Gly Gln Leu Gly Arg Val 
1               5                   10                  15      


Met Arg Glu Arg Leu Ala Pro Met Ala Glu Ile Leu Arg Leu Ala Asp 
            20                  25                  30          


Leu Ser Pro Leu Asp Pro Ala Gly Pro Asn Glu Glu Cys Val Gln Cys 
        35                  40                  45              


Asp Leu Ala Asp Ala Asn Ala Val Asn Ala Met Val Ala Gly Cys Asp 
    50                  55                  60                  


Gly Ile Val His Leu Gly Gly Ile Ser Val Glu Lys Pro Phe Glu Gln 
65                  70                  75                  80  


Ile Leu Gln Gly Asn Ile Ile Gly Leu Tyr Asn Leu Tyr Glu Ala Ala 
                85                  90                  95      


Arg Ala His Gly Gln Pro Arg Ile Val Phe Ala Ser Ser Asn His Thr 
            100                 105                 110         


Ile Gly Tyr Tyr Pro Gln Thr Glu Arg Leu Gly Pro Asp Val Pro Ala 
        115                 120                 125             


Arg Pro Asp Gly Leu Tyr Gly Val Ser Lys Cys Phe Gly Glu Asn Leu 
    130                 135                 140                 


Ala Arg Met Tyr Phe Asp Lys Phe Gly Gln Glu Thr Ala Leu Val Arg 
145                 150                 155                 160 


Ile Gly Ser Cys Thr Pro Glu Pro Asn Asn Tyr Arg Met Leu Ser Thr 
                165                 170                 175     


Trp Phe Ser His Asp Asp Phe Val Ser Leu Ile Glu Ala Val Phe Arg 
            180                 185                 190         


Ala Pro Val Leu Gly Cys Pro Val Val Trp Gly Ala Ser Ala Asn Asp 
        195                 200                 205             


Ala Gly Trp Trp Asp Asn Ser His Leu Gly Phe Leu Gly Trp Lys Pro 
    210                 215                 220                 


Lys Asp Asn Ala Glu Ala Phe Arg Arg His Ile Thr Glu Thr Thr Pro 
225                 230                 235                 240 


Pro Pro Asp Pro Asn Asp Ala Leu Val Arg Phe Gln Gly Gly Thr Phe 
                245                 250                 255     


Val Asp Asn Pro Ile Phe Lys Gln Ser 
            260                 265 


<210>  2
<211>  442
<212>  PRT
<213>  Actinobacillus succinogenes

<400>  2

Met Ser Thr Pro Ile Ile Thr Glu Met Gln Val Ile Pro Val Ala Gly 
1               5                   10                  15      


His Asp Ser Met Leu Leu Asn Leu Ser Gly Ala His Ser Pro Tyr Phe 
            20                  25                  30          


Thr Arg Asn Ile Val Ile Leu Lys Asp Asn Ser Gly Asn Thr Gly Val 
        35                  40                  45              


Gly Glu Val Pro Gly Gly Glu Lys Ile Arg Gln Thr Leu Glu Asp Ala 
    50                  55                  60                  


Lys Pro Leu Val Ile Gly Lys Thr Leu Gly Glu Tyr Lys Asn Val Met 
65                  70                  75                  80  


Asn Thr Val Arg Gln Thr Phe Asn Asp His Asp Ala Gly Gly Arg Gly 
                85                  90                  95      


Leu Gln Thr Phe Asp Leu Arg Thr Thr Ile His Val Val Thr Ala Ile 
            100                 105                 110         


Glu Ala Ala Met Leu Asp Leu Leu Gly Gln Phe Leu Gly Val Thr Val 
        115                 120                 125             


Ala Ser Leu Leu Gly Asp Gly Gln Gln Arg Asp Ala Val Glu Met Leu 
    130                 135                 140                 


Gly Tyr Leu Phe Phe Ile Gly Asp Arg Lys Lys Thr Thr Leu Ala Tyr 
145                 150                 155                 160 


Gln Asn Gln Glu Asn Asp Pro Cys Asp Trp Tyr Arg Val Arg His Glu 
                165                 170                 175     


Glu Ala Met Thr Pro Glu Ser Val Val Arg Leu Ala Glu Ala Ala Tyr 
            180                 185                 190         


Glu Lys Tyr Gly Phe Asn Asp Phe Lys Leu Lys Gly Gly Val Leu Asp 
        195                 200                 205             


Gly Phe Glu Glu Ala Glu Ala Val Thr Ala Leu Ala Lys Arg Phe Pro 
    210                 215                 220                 


Asp Ala Arg Ile Thr Leu Asp Pro Asn Gly Ala Trp Ser Leu Asp Glu 
225                 230                 235                 240 


Ala Val Lys Ile Gly Lys Gln Leu Lys Gly Val Leu Ala Tyr Ala Glu 
                245                 250                 255     


Asp Pro Cys Gly Ala Glu Gln Gly Tyr Ser Gly Arg Glu Ile Met Ala 
            260                 265                 270         


Glu Phe Arg Arg Ala Thr Gly Leu Pro Thr Ala Thr Asn Met Ile Ala 
        275                 280                 285             


Thr Asp Trp Arg Gln Met Gly His Thr Ile Ser Leu Gln Ser Val Asp 
    290                 295                 300                 


Ile Pro Leu Ala Asp Pro His Phe Trp Thr Met Gln Gly Ser Ile Arg 
305                 310                 315                 320 


Val Ala Gln Met Cys His Glu Trp Gly Leu Thr Trp Gly Ser His Ser 
                325                 330                 335     


Asn Asn His Phe Asp Ile Ser Leu Ala Met Phe Thr His Val Ala Ala 
            340                 345                 350         


Ala Ala Pro Gly Asp Ile Thr Ala Ile Asp Thr His Trp Ile Trp Gln 
        355                 360                 365             


Glu Gly Asn Gln Arg Leu Thr Lys Glu Pro Phe Gln Ile Lys Gly Gly 
    370                 375                 380                 


Leu Val Glu Val Pro Lys Lys Pro Gly Leu Gly Val Glu Leu Asp Met 
385                 390                 395                 400 


Asp Gln Val Met Lys Ala Asn Glu Leu Tyr Lys Ser Met Gly Leu Gly 
                405                 410                 415     


Ala Arg Asp Asp Ala Met Ala Met Gln Phe Leu Ile Pro Gly Trp Lys 
            420                 425                 430         


Phe Asp Asn Lys Lys Pro Cys Leu Val Arg 
        435                 440         


<210>  3
<211>  303
<212>  PRT
<213>  Acinetobacter baylyi

<400>  3

Met Asp Ala Leu Glu Leu Lys Asn Ile Val Ser Asp Gly Leu Leu Ser 
1               5                   10                  15      


Phe Pro Val Thr Asp Phe Asp Gln Asn Gly Asp Phe Asn Ala Ala Ser 
            20                  25                  30          


Tyr Ala Lys Arg Leu Glu Trp Leu Ala Pro Tyr Gly Ala Ser Ala Leu 
        35                  40                  45              


Phe Ala Ala Gly Gly Thr Gly Glu Phe Phe Ser Leu Thr Gly Asp Glu 
    50                  55                  60                  


Tyr Ser Asp Val Ile Lys Thr Ala Val Asp Ala Cys Lys Gly Ser Val 
65                  70                  75                  80  


Pro Ile Ile Ala Gly Ala Gly Gly Pro Thr Arg Gln Ala Ile Leu Gln 
                85                  90                  95      


Ala Gln Glu Ala Glu Arg Leu Gly Ala His Gly Ile Leu Leu Met Pro 
            100                 105                 110         


His Tyr Leu Thr Glu Ala Ser Gln Glu Gly Leu Val Glu His Val Lys 
        115                 120                 125             


Gln Val Cys Asn Ala Val Asn Phe Gly Val Ile Phe Tyr Asn Arg Ser 
    130                 135                 140                 


Val Ser Lys Leu Asn Val Asp Ser Leu Gln Gln Leu Val Glu Ser Cys 
145                 150                 155                 160 


Pro Asn Leu Ile Gly Phe Lys Asp Ser Ser Gly Gln Ile Asp Met Met 
                165                 170                 175     


Thr Glu Val Val Gln Thr Leu Gly Asp Arg Leu Ser Tyr Leu Gly Gly 
            180                 185                 190         


Leu Pro Thr Ala Glu Ile Phe Ala Ala Pro Tyr Lys Ala Leu Gly Ser 
        195                 200                 205             


Pro Val Tyr Ser Ser Ala Val Phe Asn Phe Ile Pro Lys Thr Ala Met 
    210                 215                 220                 


Glu Phe Tyr Asn Ala Leu Arg Asn Asp Asp Phe Ala Thr Thr Gln Arg 
225                 230                 235                 240 


Leu Ile Arg Asp Phe Phe Leu Pro Leu Ile Lys Ile Arg Asn Arg Lys 
                245                 250                 255     


Ser Gly Tyr Ala Val Ser Met Val Lys Ala Gly Ala Lys Ile Val Gly 
            260                 265                 270         


His Asp Ala Gly Pro Val Arg Pro Pro Leu Ser Asp Leu Thr Pro Gln 
        275                 280                 285             


Asp Tyr Glu Asp Leu Ala Ala Leu Ile Ala Thr Leu Gly Pro Gln 
    290                 295                 300             


<210>  4
<211>  353
<212>  PRT
<213>  Escherichia coli

<400>  4

Met Leu Tyr Thr Ser Gln Thr Thr Pro Glu Lys Asp Gln Lys Met Ser 
1               5                   10                  15      


Met Ile Lys Ser Tyr Ala Ala Lys Glu Ala Gly Gly Glu Leu Glu Val 
            20                  25                  30          


Tyr Glu Tyr Asp Pro Gly Glu Leu Arg Pro Gln Asp Val Glu Val Gln 
        35                  40                  45              


Val Asp Tyr Cys Gly Ile Cys His Ser Asp Leu Ser Met Ile Asp Asn 
    50                  55                  60                  


Glu Trp Gly Phe Ser Gln Tyr Pro Leu Val Ala Gly His Glu Val Ile 
65                  70                  75                  80  


Gly Arg Val Val Ala Leu Gly Ser Ala Ala Gln Asp Lys Gly Leu Gln 
                85                  90                  95      


Val Gly Gln Arg Val Gly Ile Gly Trp Thr Ala Arg Ser Cys Gly His 
            100                 105                 110         


Cys Asp Ala Cys Ile Ser Gly Asn Gln Ile Asn Cys Glu Gln Gly Ala 
        115                 120                 125             


Val Pro Thr Ile Met Asn Arg Gly Gly Phe Ala Glu Lys Leu Arg Ala 
    130                 135                 140                 


Asp Trp Gln Trp Val Ile Pro Leu Pro Glu Asn Ile Asp Ile Glu Ser 
145                 150                 155                 160 


Ala Gly Pro Leu Leu Cys Gly Gly Ile Thr Val Phe Lys Pro Leu Leu 
                165                 170                 175     


Met His His Ile Thr Ala Thr Ser Arg Val Gly Val Ile Gly Ile Gly 
            180                 185                 190         


Gly Leu Gly His Ile Ala Ile Lys Leu Leu His Ala Met Gly Cys Glu 
        195                 200                 205             


Val Thr Ala Phe Ser Ser Asn Pro Ala Lys Glu Gln Glu Val Leu Ala 
    210                 215                 220                 


Met Gly Ala Asp Lys Val Val Asn Ser Arg Asp Pro Gln Ala Leu Lys 
225                 230                 235                 240 


Ala Leu Ala Gly Gln Phe Asp Leu Ile Ile Asn Thr Val Asn Val Ser 
                245                 250                 255     


Leu Asp Trp Gln Pro Tyr Phe Glu Ala Leu Thr Tyr Gly Gly Asn Phe 
            260                 265                 270         


His Thr Val Gly Ala Val Leu Thr Pro Leu Ser Val Pro Ala Phe Thr 
        275                 280                 285             


Leu Ile Ala Gly Asp Arg Ser Val Ser Gly Ser Ala Thr Gly Thr Pro 
    290                 295                 300                 


Tyr Glu Leu Arg Lys Leu Met Arg Phe Ala Ala Arg Ser Lys Val Ala 
305                 310                 315                 320 


Pro Thr Thr Glu Leu Phe Pro Met Ser Lys Ile Asn Asp Ala Ile Gln 
                325                 330                 335     


His Val Arg Asp Gly Lys Ala Arg Tyr Arg Val Val Leu Lys Ala Asp 
            340                 345                 350         


Phe 
    


<210>  5
<211>  547
<212>  PRT
<213>  Lactococcus lactis

<400>  5

Met Tyr Thr Val Gly Asp Tyr Leu Leu Asp Arg Leu His Glu Leu Gly 
1               5                   10                  15      


Ile Glu Glu Ile Phe Gly Val Pro Gly Asp Tyr Asn Leu Gln Phe Leu 
            20                  25                  30          


Asp Gln Ile Ile Ser Arg Glu Asp Met Lys Trp Ile Gly Asn Ala Asn 
        35                  40                  45              


Glu Leu Asn Ala Ser Tyr Met Ala Asp Gly Tyr Ala Arg Thr Lys Lys 
    50                  55                  60                  


Ala Ala Ala Phe Leu Thr Thr Phe Gly Val Gly Glu Leu Ser Ala Ile 
65                  70                  75                  80  


Asn Gly Leu Ala Gly Ser Tyr Ala Glu Asn Leu Pro Val Val Glu Ile 
                85                  90                  95      


Val Gly Ser Pro Thr Ser Lys Val Gln Asn Asp Gly Lys Phe Val His 
            100                 105                 110         


His Thr Leu Ala Asp Gly Asp Phe Lys His Phe Met Lys Met His Glu 
        115                 120                 125             


Pro Val Thr Ala Ala Arg Thr Leu Leu Thr Ala Glu Asn Ala Thr Tyr 
    130                 135                 140                 


Glu Ile Asp Arg Val Leu Ser Gln Leu Leu Lys Glu Arg Lys Pro Val 
145                 150                 155                 160 


Tyr Ile Asn Leu Pro Val Asp Val Ala Ala Ala Lys Ala Glu Lys Pro 
                165                 170                 175     


Ala Leu Ser Leu Glu Lys Glu Ser Ser Thr Thr Asn Thr Thr Glu Gln 
            180                 185                 190         


Val Ile Leu Ser Lys Ile Glu Glu Ser Leu Lys Asn Ala Gln Lys Pro 
        195                 200                 205             


Val Val Ile Ala Gly His Glu Val Ile Ser Phe Gly Leu Glu Lys Thr 
    210                 215                 220                 


Val Thr Gln Phe Val Ser Glu Thr Lys Leu Pro Ile Thr Thr Leu Asn 
225                 230                 235                 240 


Phe Gly Lys Ser Ala Val Asp Glu Ser Leu Pro Ser Phe Leu Gly Ile 
                245                 250                 255     


Tyr Asn Gly Lys Leu Ser Glu Ile Ser Leu Lys Asn Phe Val Glu Ser 
            260                 265                 270         


Ala Asp Phe Ile Leu Met Leu Gly Val Lys Leu Thr Asp Ser Ser Thr 
        275                 280                 285             


Gly Ala Phe Thr His His Leu Asp Glu Asn Lys Met Ile Ser Leu Asn 
    290                 295                 300                 


Ile Asp Glu Gly Ile Ile Phe Asn Lys Val Val Glu Asp Phe Asp Phe 
305                 310                 315                 320 


Arg Ala Val Val Ser Ser Leu Ser Glu Leu Lys Gly Ile Glu Tyr Glu 
                325                 330                 335     


Gly Gln Tyr Ile Asp Lys Gln Tyr Glu Glu Phe Ile Pro Ser Ser Ala 
            340                 345                 350         


Pro Leu Ser Gln Asp Arg Leu Trp Gln Ala Val Glu Ser Leu Thr Gln 
        355                 360                 365             


Ser Asn Glu Thr Ile Val Ala Glu Gln Gly Thr Ser Phe Phe Gly Ala 
    370                 375                 380                 


Ser Thr Ile Phe Leu Lys Ser Asn Ser Arg Phe Ile Gly Gln Pro Leu 
385                 390                 395                 400 


Trp Gly Ser Ile Gly Tyr Thr Phe Pro Ala Ala Leu Gly Ser Gln Ile 
                405                 410                 415     


Ala Asp Lys Glu Ser Arg His Leu Leu Phe Ile Gly Asp Gly Ser Leu 
            420                 425                 430         


Gln Leu Thr Val Gln Glu Leu Gly Leu Ser Ile Arg Glu Lys Leu Asn 
        435                 440                 445             


Pro Ile Cys Phe Ile Ile Asn Asn Asp Gly Tyr Thr Val Glu Arg Glu 
    450                 455                 460                 


Ile His Gly Pro Thr Gln Ser Tyr Asn Asp Ile Pro Met Trp Asn Tyr 
465                 470                 475                 480 


Ser Lys Leu Pro Glu Thr Phe Gly Ala Thr Glu Asp Arg Val Val Ser 
                485                 490                 495     


Lys Ile Val Arg Thr Glu Asn Glu Phe Val Ser Val Met Lys Glu Ala 
            500                 505                 510         


Gln Ala Asp Val Asn Arg Met Tyr Trp Ile Glu Leu Val Leu Glu Lys 
        515                 520                 525             


Glu Asp Ala Pro Lys Leu Leu Lys Lys Met Gly Lys Leu Phe Ala Glu 
    530                 535                 540                 


Gln Asn Lys 
545         


<210>  6
<211>  386
<212>  PRT
<213>  Escherichia coli

<400>  6

Met Asn Asn Phe Asn Leu His Thr Pro Thr Arg Ile Leu Phe Gly Lys 
1               5                   10                  15      


Gly Ala Ile Ala Gly Leu Arg Glu Gln Ile Pro His Asp Ala Arg Val 
            20                  25                  30          


Leu Ile Thr Tyr Gly Gly Gly Ser Val Lys Lys Thr Gly Val Leu Asp 
        35                  40                  45              


Gln Val Leu Asp Ala Leu Lys Gly Met Asp Val Leu Glu Phe Gly Gly 
    50                  55                  60                  


Ile Glu Pro Asn Pro Ala Tyr Glu Thr Leu Met Asn Ala Val Lys Leu 
65                  70                  75                  80  


Val Arg Glu Gln Lys Val Thr Phe Leu Leu Ala Val Gly Gly Gly Ser 
                85                  90                  95      


Val Leu Asp Gly Thr Lys Phe Ile Ala Ala Ala Ala Asn Tyr Pro Glu 
            100                 105                 110         


Asn Ile Asp Pro Trp His Ile Leu Gln Thr Gly Gly Lys Glu Ile Lys 
        115                 120                 125             


Ser Ala Ile Pro Met Gly Cys Val Leu Thr Leu Pro Ala Thr Gly Ser 
    130                 135                 140                 


Glu Ser Asn Ala Gly Ala Val Ile Ser Arg Lys Thr Thr Gly Asp Lys 
145                 150                 155                 160 


Gln Ala Phe His Ser Ala His Val Gln Pro Val Phe Ala Val Leu Asp 
                165                 170                 175     


Pro Val Tyr Thr Tyr Thr Leu Pro Pro Arg Gln Val Ala Asn Gly Val 
            180                 185                 190         


Val Asp Ala Phe Val His Thr Val Glu Gln Tyr Val Thr Lys Pro Val 
        195                 200                 205             


Asp Ala Lys Ile Gln Asp Arg Phe Ala Glu Gly Ile Leu Leu Thr Leu 
    210                 215                 220                 


Ile Glu Asp Gly Pro Lys Ala Leu Lys Glu Pro Glu Asn Tyr Asp Val 
225                 230                 235                 240 


Arg Ala Asn Val Met Trp Ala Ala Thr Gln Ala Leu Asn Gly Leu Ile 
                245                 250                 255     


Gly Ala Gly Val Pro Gln Asp Trp Ala Thr His Met Leu Gly His Glu 
            260                 265                 270         


Leu Thr Ala Met His Gly Leu Asp His Ala Gln Thr Leu Ala Ile Val 
        275                 280                 285             


Leu Pro Ala Leu Trp Asn Glu Lys Arg Asp Thr Lys Arg Ala Lys Leu 
    290                 295                 300                 


Leu Gln Tyr Ala Glu Arg Val Trp Asn Ile Thr Glu Gly Ser Asp Asp 
305                 310                 315                 320 


Glu Arg Ile Asp Ala Ala Ile Ala Ala Thr Arg Asn Phe Phe Glu Gln 
                325                 330                 335     


Leu Gly Val Pro His Leu Ser Asp Tyr Gly Leu Asp Gly Ser Ser Ile 
            340                 345                 350         


Pro Ala Leu Leu Lys Lys Leu Glu Glu His Gly Met Thr Gln Leu Gly 
        355                 360                 365             


Glu Asn His Asp Ile Thr Leu Asp Val Ser Arg Arg Ile Tyr Glu Ala 
    370                 375                 380                 


Ala Arg 
385     


