                         SEQUENCE LISTING

<110>  Intercell AG
 
<120>  NOVEL OprF/I FUSION PROTEINS, THEIR PREPARATION AND USE

<130>  ICP098/PCT

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  350
<212>  PRT
<213>  Pseudomonas aeruginosa

<400>  1

Met Lys Leu Lys Asn Thr Leu Gly Val Val Ile Gly Ser Leu Val Ala 
1               5                   10                  15      


Ala Ser Ala Met Asn Ala Phe Ala Gln Gly Gln Asn Ser Val Glu Ile 
            20                  25                  30          


Glu Ala Phe Gly Lys Arg Tyr Phe Thr Asp Ser Val Arg Asn Met Lys 
        35                  40                  45              


Asn Ala Asp Leu Tyr Gly Gly Ser Ile Gly Tyr Phe Leu Thr Asp Asp 
    50                  55                  60                  


Val Glu Leu Ala Leu Ser Tyr Gly Glu Tyr His Asp Val Arg Gly Thr 
65                  70                  75                  80  


Tyr Glu Thr Gly Asn Lys Lys Val His Gly Asn Leu Thr Ser Leu Asp 
                85                  90                  95      


Ala Ile Tyr His Phe Gly Thr Pro Gly Val Gly Leu Arg Pro Tyr Val 
            100                 105                 110         


Ser Ala Gly Leu Ala His Gln Asn Ile Thr Asn Ile Asn Ser Asp Ser 
        115                 120                 125             


Gln Gly Arg Gln Gln Met Thr Met Ala Asn Ile Gly Ala Gly Leu Lys 
    130                 135                 140                 


Tyr Tyr Phe Thr Glu Asn Phe Phe Ala Lys Ala Ser Leu Asp Gly Gln 
145                 150                 155                 160 


Tyr Gly Leu Glu Lys Arg Asp Asn Gly His Gln Gly Glu Trp Met Ala 
                165                 170                 175     


Gly Leu Gly Val Gly Phe Asn Phe Gly Gly Ser Lys Ala Ala Pro Ala 
            180                 185                 190         


Pro Glu Pro Val Ala Asp Val Cys Ser Asp Ser Asp Asn Asp Gly Val 
        195                 200                 205             


Cys Asp Asn Val Asp Lys Cys Pro Asp Thr Pro Ala Asn Val Thr Val 
    210                 215                 220                 


Asp Ala Asn Gly Cys Pro Ala Val Ala Glu Val Val Arg Val Gln Leu 
225                 230                 235                 240 


Asp Val Lys Phe Asp Phe Asp Lys Ser Lys Val Lys Glu Asn Ser Tyr 
                245                 250                 255     


Ala Asp Ile Lys Asn Leu Ala Asp Phe Met Lys Gln Tyr Pro Ser Thr 
            260                 265                 270         


Ser Thr Thr Val Glu Gly His Thr Asp Ser Val Gly Thr Asp Ala Tyr 
        275                 280                 285             


Asn Gln Lys Leu Ser Glu Arg Arg Ala Asn Ala Val Arg Asp Val Leu 
    290                 295                 300                 


Val Asn Glu Tyr Gly Val Glu Gly Gly Arg Val Asn Ala Val Gly Tyr 
305                 310                 315                 320 


Gly Glu Ser Arg Pro Val Ala Asp Asn Ala Thr Ala Glu Gly Arg Ala 
                325                 330                 335     


Ile Asn Arg Arg Val Glu Ala Glu Val Glu Ala Glu Ala Lys 
            340                 345                 350 


<210>  2
<211>  83
<212>  PRT
<213>  Pseudomonas aeruginosa

<400>  2

Met Asn Asn Val Leu Lys Phe Ser Ala Leu Ala Leu Ala Ala Val Leu 
1               5                   10                  15      


Ala Thr Gly Cys Ser Ser His Ser Lys Glu Thr Glu Ala Arg Leu Thr 
            20                  25                  30          


Ala Thr Glu Asp Ala Ala Ala Arg Ala Gln Ala Arg Ala Asp Glu Ala 
        35                  40                  45              


Tyr Arg Lys Ala Asp Glu Ala Leu Gly Ala Ala Gln Lys Ala Gln Gln 
    50                  55                  60                  


Thr Ala Asp Glu Ala Asn Glu Arg Ala Leu Arg Met Leu Glu Lys Ala 
65                  70                  75                  80  


Ser Arg Lys 
            


<210>  3
<211>  224
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OprF/I with N-tag plus Met

<400>  3

Met Ala His His His His His His Ala Pro Ala Pro Glu Pro Val Ala 
1               5                   10                  15      


Asp Val Cys Ser Asp Ser Asp Asn Asp Gly Val Cys Asp Asn Val Asp 
            20                  25                  30          


Lys Cys Pro Asp Thr Pro Ala Asn Val Thr Val Asp Ala Asn Gly Cys 
        35                  40                  45              


Pro Ala Val Ala Glu Val Val Arg Val Gln Leu Asp Val Lys Phe Asp 
    50                  55                  60                  


Phe Asp Lys Ser Lys Val Lys Glu Asn Ser Tyr Ala Asp Ile Lys Asn 
65                  70                  75                  80  


Leu Ala Asp Phe Met Lys Gln Tyr Pro Ser Thr Ser Thr Thr Val Glu 
                85                  90                  95      


Gly His Thr Asp Ser Val Gly Thr Asp Ala Tyr Asn Gln Lys Leu Ser 
            100                 105                 110         


Glu Arg Arg Ala Asn Ala Val Arg Asp Val Leu Val Asn Glu Tyr Gly 
        115                 120                 125             


Val Glu Gly Gly Arg Val Asn Ala Val Gly Tyr Gly Glu Ser Arg Pro 
    130                 135                 140                 


Val Ala Asp Asn Ala Thr Ala Glu Gly Arg Ala Ile Asn Arg Arg Val 
145                 150                 155                 160 


Glu Ser Ser His Ser Lys Glu Thr Glu Ala Arg Leu Thr Ala Thr Glu 
                165                 170                 175     


Asp Ala Ala Ala Arg Ala Gln Ala Arg Ala Asp Glu Ala Tyr Arg Lys 
            180                 185                 190         


Ala Asp Glu Ala Leu Gly Ala Ala Gln Lys Ala Gln Gln Thr Ala Asp 
        195                 200                 205             


Glu Ala Asn Glu Arg Ala Leu Arg Met Leu Glu Lys Ala Ser Arg Lys 
    210                 215                 220                 


<210>  4
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OprF/I with N-tag without Met

<400>  4

Ala His His His His His His Ala Pro Ala Pro Glu Pro Val Ala Asp 
1               5                   10                  15      


Val Cys Ser Asp Ser Asp Asn Asp Gly Val Cys Asp Asn Val Asp Lys 
            20                  25                  30          


Cys Pro Asp Thr Pro Ala Asn Val Thr Val Asp Ala Asn Gly Cys Pro 
        35                  40                  45              


Ala Val Ala Glu Val Val Arg Val Gln Leu Asp Val Lys Phe Asp Phe 
    50                  55                  60                  


Asp Lys Ser Lys Val Lys Glu Asn Ser Tyr Ala Asp Ile Lys Asn Leu 
65                  70                  75                  80  


Ala Asp Phe Met Lys Gln Tyr Pro Ser Thr Ser Thr Thr Val Glu Gly 
                85                  90                  95      


His Thr Asp Ser Val Gly Thr Asp Ala Tyr Asn Gln Lys Leu Ser Glu 
            100                 105                 110         


Arg Arg Ala Asn Ala Val Arg Asp Val Leu Val Asn Glu Tyr Gly Val 
        115                 120                 125             


Glu Gly Gly Arg Val Asn Ala Val Gly Tyr Gly Glu Ser Arg Pro Val 
    130                 135                 140                 


Ala Asp Asn Ala Thr Ala Glu Gly Arg Ala Ile Asn Arg Arg Val Glu 
145                 150                 155                 160 


Ser Ser His Ser Lys Glu Thr Glu Ala Arg Leu Thr Ala Thr Glu Asp 
                165                 170                 175     


Ala Ala Ala Arg Ala Gln Ala Arg Ala Asp Glu Ala Tyr Arg Lys Ala 
            180                 185                 190         


Asp Glu Ala Leu Gly Ala Ala Gln Lys Ala Gln Gln Thr Ala Asp Glu 
        195                 200                 205             


Ala Asn Glu Arg Ala Leu Arg Met Leu Glu Lys Ala Ser Arg Lys 
    210                 215                 220             


<210>  5
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-tag plus Met

<400>  5

Met Ala His His His His His His 
1               5               


<210>  6
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-tag without Met

<400>  6

Ala His His His His His His 
1               5           


<210>  7
<211>  21
<212>  PRT
<213>  Escherichia coli

<400>  7

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala 
            20      


<210>  8
<211>  24
<212>  PRT
<213>  Pseudomonas aeruginosa

<400>  8

Met Lys Leu Lys Asn Thr Leu Gly Val Val Ile Gly Ser Leu Val Ala 
1               5                   10                  15      


Ala Ser Ala Met Ala Ala Phe Ala 
            20                  


<210>  9
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ala-(His)6-OprF190-342-OprI21-83, disulfide bond between residues
       18-27


<220>
<221>  DISULFID
<222>  (18)..(27)

<400>  9

Ala His His His His His His Ala Pro Ala Pro Glu Pro Val Ala Asp 
1               5                   10                  15      


Val Cys Ser Asp Ser Asp Asn Asp Gly Val Cys Asp Asn Val Asp Lys 
            20                  25                  30          


Cys Pro Asp Thr Pro Ala Asn Val Thr Val Asp Ala Asn Gly Cys Pro 
        35                  40                  45              


Ala Val Ala Glu Val Val Arg Val Gln Leu Asp Val Lys Phe Asp Phe 
    50                  55                  60                  


Asp Lys Ser Lys Val Lys Glu Asn Ser Tyr Ala Asp Ile Lys Asn Leu 
65                  70                  75                  80  


Ala Asp Phe Met Lys Gln Tyr Pro Ser Thr Ser Thr Thr Val Glu Gly 
                85                  90                  95      


His Thr Asp Ser Val Gly Thr Asp Ala Tyr Asn Gln Lys Leu Ser Glu 
            100                 105                 110         


Arg Arg Ala Asn Ala Val Arg Asp Val Leu Val Asn Glu Tyr Gly Val 
        115                 120                 125             


Glu Gly Gly Arg Val Asn Ala Val Gly Tyr Gly Glu Ser Arg Pro Val 
    130                 135                 140                 


Ala Asp Asn Ala Thr Ala Glu Gly Arg Ala Ile Asn Arg Arg Val Glu 
145                 150                 155                 160 


Ser Ser His Ser Lys Glu Thr Glu Ala Arg Leu Thr Ala Thr Glu Asp 
                165                 170                 175     


Ala Ala Ala Arg Ala Gln Ala Arg Ala Asp Glu Ala Tyr Arg Lys Ala 
            180                 185                 190         


Asp Glu Ala Leu Gly Ala Ala Gln Lys Ala Gln Gln Thr Ala Asp Glu 
        195                 200                 205             


Ala Asn Glu Arg Ala Leu Arg Met Leu Glu Lys Ala Ser Arg Lys 
    210                 215                 220             


<210>  10
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ala-(His)6-OprF190-342-OprI21-83, disulfide bonds between 
       residues 18-27 and 33-47


<220>
<221>  DISULFID
<222>  (18)..(27)

<220>
<221>  DISULFID
<222>  (33)..(47)

<400>  10

Ala His His His His His His Ala Pro Ala Pro Glu Pro Val Ala Asp 
1               5                   10                  15      


Val Cys Ser Asp Ser Asp Asn Asp Gly Val Cys Asp Asn Val Asp Lys 
            20                  25                  30          


Cys Pro Asp Thr Pro Ala Asn Val Thr Val Asp Ala Asn Gly Cys Pro 
        35                  40                  45              


Ala Val Ala Glu Val Val Arg Val Gln Leu Asp Val Lys Phe Asp Phe 
    50                  55                  60                  


Asp Lys Ser Lys Val Lys Glu Asn Ser Tyr Ala Asp Ile Lys Asn Leu 
65                  70                  75                  80  


Ala Asp Phe Met Lys Gln Tyr Pro Ser Thr Ser Thr Thr Val Glu Gly 
                85                  90                  95      


His Thr Asp Ser Val Gly Thr Asp Ala Tyr Asn Gln Lys Leu Ser Glu 
            100                 105                 110         


Arg Arg Ala Asn Ala Val Arg Asp Val Leu Val Asn Glu Tyr Gly Val 
        115                 120                 125             


Glu Gly Gly Arg Val Asn Ala Val Gly Tyr Gly Glu Ser Arg Pro Val 
    130                 135                 140                 


Ala Asp Asn Ala Thr Ala Glu Gly Arg Ala Ile Asn Arg Arg Val Glu 
145                 150                 155                 160 


Ser Ser His Ser Lys Glu Thr Glu Ala Arg Leu Thr Ala Thr Glu Asp 
                165                 170                 175     


Ala Ala Ala Arg Ala Gln Ala Arg Ala Asp Glu Ala Tyr Arg Lys Ala 
            180                 185                 190         


Asp Glu Ala Leu Gly Ala Ala Gln Lys Ala Gln Gln Thr Ala Asp Glu 
        195                 200                 205             


Ala Asn Glu Arg Ala Leu Arg Met Leu Glu Lys Ala Ser Arg Lys 
    210                 215                 220             


<210>  11
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ala-(His)6-OprF190-342-OprI21-83, disulfide bonds between 
       residues 18-47 and 27-33


<220>
<221>  DISULFID
<222>  (18)..(47)

<220>
<221>  DISULFID
<222>  (27)..(33)

<400>  11

Ala His His His His His His Ala Pro Ala Pro Glu Pro Val Ala Asp 
1               5                   10                  15      


Val Cys Ser Asp Ser Asp Asn Asp Gly Val Cys Asp Asn Val Asp Lys 
            20                  25                  30          


Cys Pro Asp Thr Pro Ala Asn Val Thr Val Asp Ala Asn Gly Cys Pro 
        35                  40                  45              


Ala Val Ala Glu Val Val Arg Val Gln Leu Asp Val Lys Phe Asp Phe 
    50                  55                  60                  


Asp Lys Ser Lys Val Lys Glu Asn Ser Tyr Ala Asp Ile Lys Asn Leu 
65                  70                  75                  80  


Ala Asp Phe Met Lys Gln Tyr Pro Ser Thr Ser Thr Thr Val Glu Gly 
                85                  90                  95      


His Thr Asp Ser Val Gly Thr Asp Ala Tyr Asn Gln Lys Leu Ser Glu 
            100                 105                 110         


Arg Arg Ala Asn Ala Val Arg Asp Val Leu Val Asn Glu Tyr Gly Val 
        115                 120                 125             


Glu Gly Gly Arg Val Asn Ala Val Gly Tyr Gly Glu Ser Arg Pro Val 
    130                 135                 140                 


Ala Asp Asn Ala Thr Ala Glu Gly Arg Ala Ile Asn Arg Arg Val Glu 
145                 150                 155                 160 


Ser Ser His Ser Lys Glu Thr Glu Ala Arg Leu Thr Ala Thr Glu Asp 
                165                 170                 175     


Ala Ala Ala Arg Ala Gln Ala Arg Ala Asp Glu Ala Tyr Arg Lys Ala 
            180                 185                 190         


Asp Glu Ala Leu Gly Ala Ala Gln Lys Ala Gln Gln Thr Ala Asp Glu 
        195                 200                 205             


Ala Asn Glu Arg Ala Leu Arg Met Leu Glu Lys Ala Ser Arg Lys 
    210                 215                 220             


