                         SEQUENCE LISTING

<110>  Advaxis
 
<120>  LISTERIA-BASED ADJUVANTS

<130>  P-74812-PC

<140>  PCT US12/999,999
<141>  2012-03-12

<150>  61/451,651
<151>  2011-03-11

<150>  13/290,783
<151>  2011-11-07

<150>  13/210,696
<151>  2011-08-16

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  1329
<212>  DNA
<213>  Listeria monocytogenes

<400>  1
atgaaaaaaa taatgctagt ttttattaca cttatattag ttagtctacc aattgcgcaa       60

caaactgaag caaaggatgc atctgcattc aataaagaaa attcaatttc atccatggca      120

ccaccagcat ctccgcctgc aagtcctaag acgccaatcg aaaagaaaca cgcggatgaa      180

atcgataagt atatacaagg attggattac aataaaaaca atgtattagt ataccacgga      240

gatgcagtga caaatgtgcc gccaagaaaa ggttacaaag atggaaatga atatattgtt      300

gtggagaaaa agaagaaatc catcaatcaa aataatgcag acattcaagt tgtgaatgca      360

atttcgagcc taacctatcc aggtgctctc gtaaaagcga attcggaatt agtagaaaat      420

caaccagatg ttctccctgt aaaacgtgat tcattaacac tcagcattga tttgccaggt      480

atgactaatc aagacaataa aatagttgta aaaaatgcca ctaaatcaaa cgttaacaac      540

gcagtaaata cattagtgga aagatggaat gaaaaatatg ctcaagctta tccaaatgta      600

agtgcaaaaa ttgattatga tgacgaaatg gcttacagtg aatcacaatt aattgcgaaa      660

tttggtacag catttaaagc tgtaaataat agcttgaatg taaacttcgg cgcaatcagt      720

gaagggaaaa tgcaagaaga agtcattagt tttaaacaaa tttactataa cgtgaatgtt      780

aatgaaccta caagaccttc cagatttttc ggcaaagctg ttactaaaga gcagttgcaa      840

gcgcttggag tgaatgcaga aaatcctcct gcatatatct caagtgtggc gtatggccgt      900

caagtttatt tgaaattatc aactaattcc catagtacta aagtaaaagc tgcttttgat      960

gctgccgtaa gcggaaaatc tgtctcaggt gatgtagaac taacaaatat catcaaaaat     1020

tcttccttca aagccgtaat ttacggaggt tccgcaaaag atgaagttca aatcatcgac     1080

ggcaacctcg gagacttacg cgatattttg aaaaaaggcg ctacttttaa tcgagaaaca     1140

ccaggagttc ccattgctta tacaacaaac ttcctaaaag acaatgaatt agctgttatt     1200

aaaaacaact cagaatatat tgaaacaact tcaaaagctt atacagatgg aaaaattaac     1260

atcgatcact ctggaggata cgttgctcaa ttcaacattt cttgggatga agtaaattat     1320

gatctcgag                                                             1329


<210>  2
<211>  442
<212>  PRT
<213>  Listeria monocytogenes

<400>  2

Met Lys Lys Ile Met Leu Val Phe Ile Thr Leu Ile Leu Val Ser Leu 
1               5                   10                  15      


Pro Ile Ala Gln Gln Thr Glu Ala Lys Asp Ala Ser Ala Phe Asn Lys 
            20                  25                  30          


Glu Asn Ser Ile Ser Ser Met Ala Pro Pro Ala Ser Pro Pro Ala Ser 
        35                  40                  45              


Pro Lys Thr Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys Tyr 
    50                  55                  60                  


Ile Gln Gly Leu Asp Tyr Asn Lys Asn Asn Val Leu Val Tyr His Gly 
65                  70                  75                  80  


Asp Ala Val Thr Asn Val Pro Pro Arg Lys Gly Tyr Lys Asp Gly Asn 
                85                  90                  95      


Glu Tyr Ile Val Val Glu Lys Lys Lys Lys Ser Ile Asn Gln Asn Asn 
            100                 105                 110         


Ala Asp Ile Gln Val Val Asn Ala Ile Ser Ser Leu Thr Tyr Pro Gly 
        115                 120                 125             


Ala Leu Val Lys Ala Asn Ser Glu Leu Val Glu Asn Gln Pro Asp Val 
    130                 135                 140                 


Leu Pro Val Lys Arg Asp Ser Leu Thr Leu Ser Ile Asp Leu Pro Gly 
145                 150                 155                 160 


Met Thr Asn Gln Asp Asn Lys Ile Val Val Lys Asn Ala Thr Lys Ser 
                165                 170                 175     


Asn Val Asn Asn Ala Val Asn Thr Leu Val Glu Arg Trp Asn Glu Lys 
            180                 185                 190         


Tyr Ala Gln Ala Tyr Pro Asn Val Ser Ala Lys Ile Asp Tyr Asp Asp 
        195                 200                 205             


Glu Met Ala Tyr Ser Glu Ser Gln Leu Ile Ala Lys Phe Gly Thr Ala 
    210                 215                 220                 


Phe Lys Ala Val Asn Asn Ser Leu Asn Val Asn Phe Gly Ala Ile Ser 
225                 230                 235                 240 


Glu Gly Lys Met Gln Glu Glu Val Ile Ser Phe Lys Gln Ile Tyr Tyr 
                245                 250                 255     


Asn Val Asn Val Asn Glu Pro Thr Arg Pro Ser Arg Phe Phe Gly Lys 
            260                 265                 270         


Ala Val Thr Lys Glu Gln Leu Gln Ala Leu Gly Val Asn Ala Glu Asn 
        275                 280                 285             


Pro Pro Ala Tyr Ile Ser Ser Val Ala Tyr Gly Arg Gln Val Tyr Leu 
    290                 295                 300                 


Lys Leu Ser Thr Asn Ser His Ser Thr Lys Val Lys Ala Ala Phe Asp 
305                 310                 315                 320 


Ala Ala Val Ser Gly Lys Ser Val Ser Gly Asp Val Glu Leu Thr Asn 
                325                 330                 335     


Ile Ile Lys Asn Ser Ser Phe Lys Ala Val Ile Tyr Gly Gly Ser Ala 
            340                 345                 350         


Lys Asp Glu Val Gln Ile Ile Asp Gly Asn Leu Gly Asp Leu Arg Asp 
        355                 360                 365             


Ile Leu Lys Lys Gly Ala Thr Phe Asn Arg Glu Thr Pro Gly Val Pro 
    370                 375                 380                 


Ile Ala Tyr Thr Thr Asn Phe Leu Lys Asp Asn Glu Leu Ala Val Ile 
385                 390                 395                 400 


Lys Asn Asn Ser Glu Tyr Ile Glu Thr Thr Ser Lys Ala Tyr Thr Asp 
                405                 410                 415     


Gly Lys Ile Asn Ile Asp His Ser Gly Gly Tyr Val Ala Gln Phe Asn 
            420                 425                 430         


Ile Ser Trp Asp Glu Val Asn Tyr Asp Leu 
        435                 440         


<210>  3
<211>  390
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated ActA

<400>  3

Met Arg Ala Met Met Val Val Phe Ile Thr Ala Asn Cys Ile Thr Ile 
1               5                   10                  15      


Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp Ser Glu Asp Ser Ser Leu 
            20                  25                  30          


Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr Glu Glu Gln Pro Ser Glu 
        35                  40                  45              


Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala Arg Glu Val Ser Ser Arg 
    50                  55                  60                  


Asp Ile Lys Glu Leu Glu Lys Ser Asn Lys Val Arg Asn Thr Asn Lys 
65                  70                  75                  80  


Ala Asp Leu Ile Ala Met Leu Lys Glu Lys Ala Glu Lys Gly Pro Asn 
                85                  90                  95      


Ile Asn Asn Asn Asn Ser Glu Gln Thr Glu Asn Ala Ala Ile Asn Glu 
            100                 105                 110         


Glu Ala Ser Gly Ala Asp Arg Pro Ala Ile Gln Val Glu Arg Arg His 
        115                 120                 125             


Pro Gly Leu Pro Ser Asp Ser Ala Ala Glu Ile Lys Lys Arg Arg Lys 
    130                 135                 140                 


Ala Ile Ala Ser Ser Asp Ser Glu Leu Glu Ser Leu Thr Tyr Pro Asp 
145                 150                 155                 160 


Lys Pro Thr Lys Val Asn Lys Lys Lys Val Ala Lys Glu Ser Val Ala 
                165                 170                 175     


Asp Ala Ser Glu Ser Asp Leu Asp Ser Ser Met Gln Ser Ala Asp Glu 
            180                 185                 190         


Ser Ser Pro Gln Pro Leu Lys Ala Asn Gln Gln Pro Phe Phe Pro Lys 
        195                 200                 205             


Val Phe Lys Lys Ile Lys Asp Ala Gly Lys Trp Val Arg Asp Lys Ile 
    210                 215                 220                 


Asp Glu Asn Pro Glu Val Lys Lys Ala Ile Val Asp Lys Ser Ala Gly 
225                 230                 235                 240 


Leu Ile Asp Gln Leu Leu Thr Lys Lys Lys Ser Glu Glu Val Asn Ala 
                245                 250                 255     


Ser Asp Phe Pro Pro Pro Pro Thr Asp Glu Glu Leu Arg Leu Ala Leu 
            260                 265                 270         


Pro Glu Thr Pro Met Leu Leu Gly Phe Asn Ala Pro Ala Thr Ser Glu 
        275                 280                 285             


Pro Ser Ser Phe Glu Phe Pro Pro Pro Pro Thr Asp Glu Glu Leu Arg 
    290                 295                 300                 


Leu Ala Leu Pro Glu Thr Pro Met Leu Leu Gly Phe Asn Ala Pro Ala 
305                 310                 315                 320 


Thr Ser Glu Pro Ser Ser Phe Glu Phe Pro Pro Pro Pro Thr Glu Asp 
                325                 330                 335     


Glu Leu Glu Ile Ile Arg Glu Thr Ala Ser Ser Leu Asp Ser Ser Phe 
            340                 345                 350         


Thr Arg Gly Asp Leu Ala Ser Leu Arg Asn Ala Ile Asn Arg His Ser 
        355                 360                 365             


Gln Asn Phe Ser Asp Phe Pro Pro Ile Pro Thr Glu Glu Glu Leu Asn 
    370                 375                 380                 


Gly Arg Gly Gly Arg Pro 
385                 390 


<210>  4
<211>  1151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated ActA

<400>  4

Ala Thr Gly Cys Gly Thr Gly Cys Gly Ala Thr Gly Ala Thr Gly Gly 
1               5                   10                  15      


Thr Gly Gly Thr Thr Thr Thr Cys Ala Thr Thr Ala Cys Thr Gly Cys 
            20                  25                  30          


Cys Ala Ala Thr Thr Gly Cys Ala Thr Thr Ala Cys Gly Ala Thr Thr 
        35                  40                  45              


Ala Ala Cys Cys Cys Cys Gly Ala Cys Ala Thr Ala Ala Thr Ala Thr 
    50                  55                  60                  


Thr Thr Gly Cys Ala Gly Cys Gly Ala Cys Ala Gly Ala Thr Ala Gly 
65                  70                  75                  80  


Cys Gly Ala Ala Gly Ala Thr Thr Cys Thr Ala Gly Thr Cys Thr Ala 
                85                  90                  95      


Ala Ala Cys Ala Cys Ala Gly Ala Thr Gly Ala Ala Thr Gly Gly Gly 
            100                 105                 110         


Ala Ala Gly Ala Ala Gly Ala Ala Ala Ala Ala Ala Cys Ala Gly Ala 
        115                 120                 125             


Ala Gly Ala Gly Cys Ala Ala Cys Cys Ala Ala Gly Cys Gly Ala Gly 
    130                 135                 140                 


Gly Thr Ala Ala Ala Thr Ala Cys Gly Gly Gly Ala Cys Cys Ala Ala 
145                 150                 155                 160 


Gly Ala Thr Ala Cys Gly Ala Ala Ala Cys Thr Gly Cys Ala Cys Gly 
                165                 170                 175     


Thr Gly Ala Ala Gly Thr Ala Ala Gly Thr Thr Cys Ala Cys Gly Thr 
            180                 185                 190         


Gly Ala Thr Ala Thr Thr Ala Ala Ala Gly Ala Ala Cys Thr Ala Gly 
        195                 200                 205             


Ala Ala Ala Ala Ala Thr Cys Gly Ala Ala Thr Ala Ala Ala Gly Thr 
    210                 215                 220                 


Gly Ala Gly Ala Ala Ala Thr Ala Cys Gly Ala Ala Cys Ala Ala Ala 
225                 230                 235                 240 


Gly Cys Ala Gly Ala Cys Cys Thr Ala Ala Thr Ala Gly Cys Ala Ala 
                245                 250                 255     


Thr Gly Thr Thr Gly Ala Ala Ala Gly Ala Ala Ala Ala Ala Gly Cys 
            260                 265                 270         


Ala Gly Ala Ala Ala Ala Ala Gly Gly Thr Cys Cys Ala Ala Ala Thr 
        275                 280                 285             


Ala Thr Cys Ala Ala Thr Ala Ala Thr Ala Ala Cys Ala Ala Cys Ala 
    290                 295                 300                 


Gly Thr Gly Ala Ala Cys Ala Ala Ala Cys Thr Gly Ala Gly Ala Ala 
305                 310                 315                 320 


Thr Gly Cys Gly Gly Cys Thr Ala Thr Ala Ala Ala Thr Gly Ala Ala 
                325                 330                 335     


Gly Ala Gly Gly Cys Thr Thr Cys Ala Gly Gly Ala Gly Cys Cys Gly 
            340                 345                 350         


Ala Cys Cys Gly Ala Cys Cys Ala Gly Cys Thr Ala Thr Ala Cys Ala 
        355                 360                 365             


Ala Gly Thr Gly Gly Ala Gly Cys Gly Thr Cys Gly Thr Cys Ala Thr 
    370                 375                 380                 


Cys Cys Ala Gly Gly Ala Thr Thr Gly Cys Cys Ala Thr Cys Gly Gly 
385                 390                 395                 400 


Ala Thr Ala Gly Cys Gly Cys Ala Gly Cys Gly Gly Ala Ala Ala Thr 
                405                 410                 415     


Thr Ala Ala Ala Ala Ala Ala Ala Gly Ala Ala Gly Gly Ala Ala Ala 
            420                 425                 430         


Gly Cys Cys Ala Thr Ala Gly Cys Ala Thr Cys Ala Thr Cys Gly Gly 
        435                 440                 445             


Ala Thr Ala Gly Thr Gly Ala Gly Cys Thr Thr Gly Ala Ala Ala Gly 
    450                 455                 460                 


Cys Cys Thr Thr Ala Cys Thr Thr Ala Thr Cys Cys Gly Gly Ala Thr 
465                 470                 475                 480 


Ala Ala Ala Cys Cys Ala Ala Cys Ala Ala Ala Ala Gly Thr Ala Ala 
                485                 490                 495     


Ala Thr Ala Ala Gly Ala Ala Ala Ala Ala Ala Gly Thr Gly Gly Cys 
            500                 505                 510         


Gly Ala Ala Ala Gly Ala Gly Thr Cys Ala Gly Thr Thr Gly Cys Gly 
        515                 520                 525             


Gly Ala Thr Gly Cys Thr Thr Cys Thr Gly Ala Ala Ala Gly Thr Gly 
    530                 535                 540                 


Ala Cys Thr Thr Ala Gly Ala Thr Thr Cys Thr Ala Gly Cys Ala Thr 
545                 550                 555                 560 


Gly Cys Ala Gly Thr Cys Ala Gly Cys Ala Gly Ala Thr Gly Ala Gly 
                565                 570                 575     


Thr Cys Thr Thr Cys Ala Cys Cys Ala Cys Ala Ala Cys Cys Thr Thr 
            580                 585                 590         


Thr Ala Ala Ala Ala Gly Cys Ala Ala Ala Cys Cys Ala Ala Cys Ala 
        595                 600                 605             


Ala Cys Cys Ala Thr Thr Thr Thr Thr Cys Cys Cys Thr Ala Ala Ala 
    610                 615                 620                 


Gly Thr Ala Thr Thr Thr Ala Ala Ala Ala Ala Ala Ala Thr Ala Ala 
625                 630                 635                 640 


Ala Ala Gly Ala Thr Gly Cys Gly Gly Gly Gly Ala Ala Ala Thr Gly 
                645                 650                 655     


Gly Gly Thr Ala Cys Gly Thr Gly Ala Thr Ala Ala Ala Ala Thr Cys 
            660                 665                 670         


Gly Ala Cys Gly Ala Ala Ala Ala Thr Cys Cys Thr Gly Ala Ala Gly 
        675                 680                 685             


Thr Ala Ala Ala Gly Ala Ala Ala Gly Cys Gly Ala Thr Thr Gly Thr 
    690                 695                 700                 


Thr Gly Ala Thr Ala Ala Ala Ala Gly Thr Gly Cys Ala Gly Gly Gly 
705                 710                 715                 720 


Thr Thr Ala Ala Thr Thr Gly Ala Cys Cys Ala Ala Thr Thr Ala Thr 
                725                 730                 735     


Thr Ala Ala Cys Cys Ala Ala Ala Ala Ala Gly Ala Ala Ala Ala Gly 
            740                 745                 750         


Thr Gly Ala Ala Gly Ala Gly Gly Thr Ala Ala Ala Thr Gly Cys Thr 
        755                 760                 765             


Thr Cys Gly Gly Ala Cys Thr Thr Cys Cys Cys Gly Cys Cys Ala Cys 
    770                 775                 780                 


Cys Ala Cys Cys Thr Ala Cys Gly Gly Ala Thr Gly Ala Ala Gly Ala 
785                 790                 795                 800 


Gly Thr Thr Ala Ala Gly Ala Cys Thr Thr Gly Cys Thr Thr Thr Gly 
                805                 810                 815     


Cys Cys Ala Gly Ala Gly Ala Cys Ala Cys Cys Ala Ala Thr Gly Cys 
            820                 825                 830         


Thr Thr Cys Thr Thr Gly Gly Thr Thr Thr Thr Ala Ala Thr Gly Cys 
        835                 840                 845             


Thr Cys Cys Thr Gly Cys Thr Ala Cys Ala Thr Cys Ala Gly Ala Ala 
    850                 855                 860                 


Cys Cys Gly Ala Gly Cys Thr Cys Ala Thr Thr Cys Gly Ala Ala Thr 
865                 870                 875                 880 


Thr Thr Cys Cys Ala Cys Cys Ala Cys Cys Ala Cys Cys Thr Ala Cys 
                885                 890                 895     


Gly Gly Ala Thr Gly Ala Ala Gly Ala Gly Thr Thr Ala Ala Gly Ala 
            900                 905                 910         


Cys Thr Thr Gly Cys Thr Thr Thr Gly Cys Cys Ala Gly Ala Gly Ala 
        915                 920                 925             


Cys Gly Cys Cys Ala Ala Thr Gly Cys Thr Thr Cys Thr Thr Gly Gly 
    930                 935                 940                 


Thr Thr Thr Thr Ala Ala Thr Gly Cys Thr Cys Cys Thr Gly Cys Thr 
945                 950                 955                 960 


Ala Cys Ala Thr Cys Gly Gly Ala Ala Cys Cys Gly Ala Gly Cys Thr 
                965                 970                 975     


Cys Gly Thr Thr Cys Gly Ala Ala Thr Thr Thr Cys Cys Ala Cys Cys 
            980                 985                 990         


Gly Cys Cys Thr Cys Cys Ala Ala  Cys Ala Gly Ala Ala  Gly Ala Thr 
        995                 1000                 1005             


Gly Ala  Ala Cys Thr Ala Gly  Ala Ala Ala Thr Cys  Ala Thr Cys 
    1010                 1015                 1020             


Cys Gly  Gly Gly Ala Ala Ala  Cys Ala Gly Cys Ala  Thr Cys Cys 
    1025                 1030                 1035             


Thr Cys  Gly Cys Thr Ala Gly  Ala Thr Thr Cys Thr  Ala Gly Thr 
    1040                 1045                 1050             


Thr Thr  Thr Ala Cys Ala Ala  Gly Ala Gly Gly Gly  Gly Ala Thr 
    1055                 1060                 1065             


Thr Thr  Ala Gly Cys Thr Ala  Gly Thr Thr Thr Gly  Ala Gly Ala 
    1070                 1075                 1080             


Ala Ala  Thr Gly Cys Thr Ala  Thr Thr Ala Ala Thr  Cys Gly Cys 
    1085                 1090                 1095             


Cys Ala  Thr Ala Gly Thr Cys  Ala Ala Ala Ala Thr  Thr Thr Cys 
    1100                 1105                 1110             


Thr Cys  Thr Gly Ala Thr Thr  Thr Cys Cys Cys Ala  Cys Cys Ala 
    1115                 1120                 1125             


Ala Thr  Cys Cys Cys Ala Ala  Cys Ala Gly Ala Ala  Gly Ala Ala 
    1130                 1135                 1140             


Gly Ala  Gly Thr Thr Gly Ala  Ala 
    1145                 1150     


<210>  5
<211>  669
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  truncated ActA

<400>  5
tttatcacgt acccatttcc ccgcatcttt tattttttta aatactttag ggaaaaatgg       60

tttttgattt gcttttaaag gttgtggtgt agactcgtct gctgactgca tgctagaatc      120

taagtcactt tcagaagcat ccacaactga ctctttcgcc acttttctct tatttgcttt      180

tgttggttta tctggataag taaggctttc aagctcacta tccgacgacg ctatggcttt      240

tcttcttttt ttaatttccg ctgcgctatc cgatgacaga cctggatgac gacgctccac      300

ttgcagagtt ggtcggtcga ctcctgaagc ctcttcattt atagccacat ttcctgtttg      360

ctcaccgttg ttattattgt tattcggacc tttctctgct tttgctttca acattgctat      420

taggtctgct ttgttcgtat ttttcacttt attcgatttt tctagttcct caatatcacg      480

tgaacttact tcacgtgcag tttcgtatct tggtcccgta tttacctcgc ttggctgctc      540

ttctgttttt tcttcttccc attcatctgt gtttagactg gaatcttcgc tatctgtcgc      600

tgcaaatatt atgtcggggt taatcgtaat gcagttggca gtaatgaaaa ctaccatcat      660

cgcacgcat                                                              669


<210>  6
<211>  390
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated ActA

<400>  6

Met Arg Ala Met Met Val Val Phe Ile Thr Ala Asn Cys Ile Thr Ile 
1               5                   10                  15      


Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp Ser Glu Asp Ser Ser Leu 
            20                  25                  30          


Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr Glu Glu Gln Pro Ser Glu 
        35                  40                  45              


Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala Arg Glu Val Ser Ser Arg 
    50                  55                  60                  


Asp Ile Lys Glu Leu Glu Lys Ser Asn Lys Val Arg Asn Thr Asn Lys 
65                  70                  75                  80  


Ala Asp Leu Ile Ala Met Leu Lys Glu Lys Ala Glu Lys Gly Pro Asn 
                85                  90                  95      


Ile Asn Asn Asn Asn Ser Glu Gln Thr Glu Asn Ala Ala Ile Asn Glu 
            100                 105                 110         


Glu Ala Ser Gly Ala Asp Arg Pro Ala Ile Gln Val Glu Arg Arg His 
        115                 120                 125             


Pro Gly Leu Pro Ser Asp Ser Ala Ala Glu Ile Lys Lys Arg Arg Lys 
    130                 135                 140                 


Ala Ile Ala Ser Ser Asp Ser Glu Leu Glu Ser Leu Thr Tyr Pro Asp 
145                 150                 155                 160 


Lys Pro Thr Lys Val Asn Lys Lys Lys Val Ala Lys Glu Ser Val Ala 
                165                 170                 175     


Asp Ala Ser Glu Ser Asp Leu Asp Ser Ser Met Gln Ser Ala Asp Glu 
            180                 185                 190         


Ser Ser Pro Gln Pro Leu Lys Ala Asn Gln Gln Pro Phe Phe Pro Lys 
        195                 200                 205             


Val Phe Lys Lys Ile Lys Asp Ala Gly Lys Trp Val Arg Asp Lys Ile 
    210                 215                 220                 


Asp Glu Asn Pro Glu Val Lys Lys Ala Ile Val Asp Lys Ser Ala Gly 
225                 230                 235                 240 


Leu Ile Asp Gln Leu Leu Thr Lys Lys Lys Ser Glu Glu Val Asn Ala 
                245                 250                 255     


Ser Asp Phe Pro Pro Pro Pro Thr Asp Glu Glu Leu Arg Leu Ala Leu 
            260                 265                 270         


Pro Glu Thr Pro Met Leu Leu Gly Phe Asn Ala Pro Ala Thr Ser Glu 
        275                 280                 285             


Pro Ser Ser Phe Glu Phe Pro Pro Pro Pro Thr Asp Glu Glu Leu Arg 
    290                 295                 300                 


Leu Ala Leu Pro Glu Thr Pro Met Leu Leu Gly Phe Asn Ala Pro Ala 
305                 310                 315                 320 


Thr Ser Glu Pro Ser Ser Phe Glu Phe Pro Pro Pro Pro Thr Glu Asp 
                325                 330                 335     


Glu Leu Glu Ile Ile Arg Glu Thr Ala Ser Ser Leu Asp Ser Ser Phe 
            340                 345                 350         


Thr Arg Gly Asp Leu Ala Ser Leu Arg Asn Ala Ile Asn Arg His Ser 
        355                 360                 365             


Gln Asn Phe Ser Asp Phe Pro Pro Ile Pro Thr Glu Glu Glu Leu Asn 
    370                 375                 380                 


Gly Arg Gly Gly Arg Pro 
385                 390 


<210>  7
<211>  1170
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  truncated ActA

<400>  7
atgcgtgcga tgatggtggt tttcattact gccaattgca ttacgattaa ccccgacata       60

atatttgcag cgacagatag cgaagattct agtctaaaca cagatgaatg ggaagaagaa      120

aaaacagaag agcaaccaag cgaggtaaat acgggaccaa gatacgaaac tgcacgtgaa      180

gtaagttcac gtgatattaa agaactagaa aaatcgaata aagtgagaaa tacgaacaaa      240

gcagacctaa tagcaatgtt gaaagaaaaa gcagaaaaag gtccaaatat caataataac      300

aacagtgaac aaactgagaa tgcggctata aatgaagagg cttcaggagc cgaccgacca      360

gctatacaag tggagcgtcg tcatccagga ttgccatcgg atagcgcagc ggaaattaaa      420

aaaagaagga aagccatagc atcatcggat agtgagcttg aaagccttac ttatccggat      480

aaaccaacaa aagtaaataa gaaaaaagtg gcgaaagagt cagttgcgga tgcttctgaa      540

agtgacttag attctagcat gcagtcagca gatgagtctt caccacaacc tttaaaagca      600

aaccaacaac catttttccc taaagtattt aaaaaaataa aagatgcggg gaaatgggta      660

cgtgataaaa tcgacgaaaa tcctgaagta aagaaagcga ttgttgataa aagtgcaggg      720

ttaattgacc aattattaac caaaaagaaa agtgaagagg taaatgcttc ggacttcccg      780

ccaccaccta cggatgaaga gttaagactt gctttgccag agacaccaat gcttcttggt      840

tttaatgctc ctgctacatc agaaccgagc tcattcgaat ttccaccacc acctacggat      900

gaagagttaa gacttgcttt gccagagacg ccaatgcttc ttggttttaa tgctcctgct      960

acatcggaac cgagctcgtt cgaatttcca ccgcctccaa cagaagatga actagaaatc     1020

atccgggaaa cagcatcctc gctagattct agttttacaa gaggggattt agctagtttg     1080

agaaatgcta ttaatcgcca tagtcaaaat ttctctgatt tcccaccaat cccaacagaa     1140

gaagagttga acgggagagg cggtagacca                                      1170


<210>  8
<211>  32
<212>  PRT
<213>  Listeria monocytogenes

<400>  8

Lys Glu Asn Ser Ile Ser Ser Met Ala Pro Pro Ala Ser Pro Pro Ala 
1               5                   10                  15      


Ser Pro Lys Thr Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys 
            20                  25                  30          


<210>  9
<211>  19
<212>  PRT
<213>  Listeria monocytogenes

<400>  9

Lys Glu Asn Ser Ile Ser Ser Met Ala Pro Pro Ala Ser Pro Pro Ala 
1               5                   10                  15      


Ser Pro Lys 
            


<210>  10
<211>  14
<212>  PRT
<213>  Listeria monocytogenes

<400>  10

Lys Thr Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg 
1               5                   10                  


<210>  11
<211>  28
<212>  PRT
<213>  Listeria monocytogenes

<400>  11

Lys Ala Ser Val Thr Asp Thr Ser Glu Gly Asp Leu Asp Ser Ser Met 
1               5                   10                  15      


Gln Ser Ala Asp Glu Ser Thr Pro Gln Pro Leu Lys 
            20                  25              


<210>  12
<211>  20
<212>  PRT
<213>  Listeria monocytogenes

<400>  12

Lys Asn Glu Glu Val Asn Ala Ser Asp Phe Pro Pro Pro Pro Thr Asp 
1               5                   10                  15      


Glu Glu Leu Arg 
            20  


<210>  13
<211>  33
<212>  PRT
<213>  Listeria monocytogenes

<400>  13

Arg Gly Gly Ile Pro Thr Ser Glu Glu Phe Ser Ser Leu Asn Ser Gly 
1               5                   10                  15      


Asp Phe Thr Asp Asp Glu Asn Ser Glu Thr Thr Glu Glu Glu Ile Asp 
            20                  25                  30          


Arg 
    


<210>  14
<211>  28
<212>  PRT
<213>  Listeria monocytogenes

<400>  14

Lys Glu Ser Val Val Asp Ala Ser Glu Ser Asp Leu Asp Ser Ser Met 
1               5                   10                  15      


Gln Ser Ala Asp Glu Ser Thr Pro Gln Pro Leu Lys 
            20                  25              


<210>  15
<211>  20
<212>  PRT
<213>  Listeria monocytogenes

<400>  15

Lys Ser Glu Glu Val Asn Ala Ser Asp Phe Pro Pro Pro Pro Thr Asp 
1               5                   10                  15      


Glu Glu Leu Arg 
            20  


<210>  16
<211>  33
<212>  PRT
<213>  Listeria monocytogenes

<400>  16

Arg Gly Gly Arg Pro Thr Ser Glu Glu Phe Ser Ser Leu Asn Ser Gly 
1               5                   10                  15      


Asp Phe Thr Asp Asp Glu Asn Ser Glu Thr Thr Glu Glu Glu Ile Asp 
            20                  25                  30          


Arg 
    


<210>  17
<211>  19
<212>  PRT
<213>  Listeria seeligeri

<400>  17

Arg Ser Glu Val Thr Ile Ser Pro Ala Glu Thr Pro Glu Ser Pro Pro 
1               5                   10                  15      


Ala Thr Pro 
            


<210>  18
<211>  17
<212>  PRT
<213>  Streptococcus pyogenes

<400>  18

Lys Gln Asn Thr Ala Ser Thr Glu Thr Thr Thr Thr Asn Glu Gln Pro 
1               5                   10                  15      


Lys 
    


<210>  19
<211>  17
<212>  PRT
<213>  Streptococcus equisimilis

<400>  19

Lys Gln Asn Thr Ala Asn Thr Glu Thr Thr Thr Thr Asn Glu Gln Pro 
1               5                   10                  15      


Lys 
    


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dal gene forward primer

<400>  20
ccatggtgac aggctggcat c                                                 21


<210>  21
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dal gene reverse primer

<400>  21
gctagcctaa tggatgtatt ttctagg                                           27


<210>  22
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  minimal p60 promoter sequence forward primer

<400>  22
ttaattaaca aatagttggt atagtcc                                           27


<210>  23
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  minimal p60 promoter sequence forward primer

<400>  23
gacgatgcca gcctgtcacc atggaaaact cctctc                                 36


<210>  24
<211>  156
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  truncated p60 promoter

<400>  24
caaatagttg gtatagtcct ctttagcctt tggagtatta tctcatcatt tgttttttag       60

gtgaaaactg ggtaaactta gtattatcaa tataaaatta attctcaaat acttaattac      120

gtactgggat tttctgaaaa aagagaggag ttttcc                                156


<210>  25
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oriRep forward primer

<400>  25
ggcgccacta actcaacgct agtag                                             25


<210>  26
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oriRep reverse primer

<400>  26
gctagccagc aaagaaaaac aaacacg                                           27


<210>  27
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oriRep forward primer

<400>  27
gtcgacggtc accggcgcca ctaactcaac gctagtag                               38


<210>  28
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oriRep reverse primer

<400>  28
ttaattaagc tagccagcaa agaaaaacaa acacg                                  35


<210>  29
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  F primer for amplifying LLO-E7 gene

<400>  29
atgaaaaaaa taatgctagt ttttattac                                         29


<210>  30
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R primer for amplifying LLO-E7 gene

<400>  30
gcggccgctt aatgatgatg atgatgatgt ggtttctgag aacagatg                    48


<210>  31
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Taqman primer-probe sets

<400>  31
gcaagtgtga ctctacgctt cg                                                22


<210>  32
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Taqman primer-probe sets

<400>  32
tgcccattaa caggtcttcc a                                                 21


<210>  33
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Taqman primer-probe sets

<400>  33
tgcgtacaaa gcacacacgt agacattcgt ac                                     32


<210>  34
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Taqman primer-probe sets

<400>  34
tgacatcgtt tgtgtttgag ctag                                              24


<210>  35
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Taqman primer-probe sets

<400>  35
gcagcgctct ctataccagg tac                                               23


<210>  36
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Taqman primer-probe sets

<400>  36
ttaatgtcca tgttatgtct ccgttatagc tcatcgta                               38


<210>  37
<211>  2015
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA fragment that are present upstream and downstream of inl C 
       region

<400>  37
atggcgcggg atggtatact atacaagcgt atggttcaaa aagatacttt gaattaagaa       60

gtacaataaa gttaacttca ttagacaaaa agaaaaaaca aggaagaata gtacatagtt      120

ataaatactt ggagagtgag gtgtaatatg ggggcagctg atttttgggg tttcatatat      180

gtagtttcaa gattagccat tgttgcggca gtagtttact tcttatactt attgagaaaa      240

attgcaaata aatagaaaaa aagccttgtc aaacgaggct ttttttatgc aaaaaatacg      300

acgaatgaag ccatgtgaga caatttggaa tagcagacaa caaggaaggt agaacatgtt      360

ttgaaaaatt tactgatttt cgattattat taacgcttgt taatttaaac atctcttatt      420

tttgctaaca tataagtata caaagggaca taaaaaggtt aacagcgttt gttaaatagg      480

aagtatatga aaatcctctt ttgtgtttct aaatttattt ttaaggagtg gagaatgttg      540

aaaaaaaata attggttaca aaatgcagta atagcaatgc tagtgttaat tgtaggtctg      600

tgcattaata tgggttctgg aacaaaagta caagctgaga gtattcaacg accaacgcct      660

attaaccaag tttttccaga tcccggccta gcgaatgcag tgaaacaaaa tttagggaag      720

caaagtgtta cagaccttgt atcacaaaag gaactatctg gagtacaaaa tttcaatgga      780

gataatagca acattcaatc tcttgcggga atgcaatttt tcactaattt aaaagaactt      840

catctatccc ataatcaaat aagtgacctt agtcctttaa aggatctaac taagttagaa      900

gagctatctg tgaatagaaa cagactgaaa aatttaaacg gaattccaag tgcttgttta      960

tctcgcttgt ttttagataa caacgaactc agagatactg actcgcttat tcatttgaaa     1020

aatctagaaa tcttatctat tcgtaataat aagttaaaaa gtattgtgat gcttggtttt     1080

ttatcaaaac tagaggtatt agatttgcat ggtaatgaaa taacaaatac aggtggacta     1140

actagattga agaaagttaa ctggatagat ttaactggtc agaaatgtgt gaatgaacca     1200

gtaaaatacc aaccagaatt gtatataaca aatactgtca aagacccaga tggaagatgg     1260

atatctccat attacatcag taatggtggg agttatgtag atggttgtgt cctgtgggaa     1320

ttgccagttt atacagatga agtaagctat aagtttagcg aatatataaa cgttggggag     1380

actgaggcta tatttgatgg aacagttaca caacctatca agaattagga cttgtgcaca     1440

cctgtatact ttgagctctc gtataatcac gagagctttt taaatatgta agtcttaatt     1500

atctcttgac aaaaagaacg tttattcgta taaggttacc aagagatgaa gaaactattt     1560

tatttacaat tcaccttgac accaaaaact ccatatgata tagtaaataa ggttattaaa     1620

caagaaagaa gaagcaaccc gcttctcgcc tcgttaacac gaacgttttc aggcaaaaaa     1680

ttcaaacttt cgtcgcgtag cttacgcgat tttgaatgtg cgggattgct gaaaagcagc     1740

ccgttttttt atggcctccg aacgaatgag ttagcaggcc gcagatttga acagctattt     1800

tctatcttgt tgtaacaaaa ttaagtggag gtggctcacc attagcaaag acatgttggt     1860

aaacgatggg attcgtgcac gtgaagtaag attgatcgac caagacggtg aacaattagg     1920

cgtgaagagt aaaatcgatg cgcttcaaat tgctgaaaag gctaatcttg atctagtgct     1980

tgttgctcca acagcgaaac cgccagtagc tcgta                                2015


<210>  38
<211>  1140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA fragment that are present upstream and downstream of inl C 
       region

<400>  38
gaattcatgg cgcgggatgg tatactatac aagcgtatgg ttcaaaaaga tactttgaat       60

taagaagtac aataaagtta acttcattag acaaaaagaa aaaacaagga agaatagtac      120

atagttataa atacttggag agtgaggtgt aatatggggg cagctgattt ttggggtttc      180

atatatgtag tttcaagatt agccattgtt gcggcagtag tttacttctt atacttattg      240

agaaaaattg caaataaata gaaaaaaagc cttgtcaaac gaggcttttt ttatgcaaaa      300

aatacgacga atgaagccat gtgagacaat ttggaatagc agacaacaag gaaggtagaa      360

catgttttga aaaatttact gattttcgat tattattaac gcttgttaat ttaaacatct      420

cttatttttg ctaacatata agtatacaaa gggacataaa aaggttaaca gcgtttgtta      480

aataggaagt atatgaaaat cctcttttgt gtttctaaat ttatttttaa ggagtggaga      540

ggatccggac ttgtgcacac ctgtatactt tgagctctcg tataatcacg agagcttttt      600

aaatatgtaa gtcttaatta tctcttgaca aaaagaacgt ttattcgtat aaggttacca      660

agagatgaag aaactatttt atttacaatt caccttgaca ccaaaaactc catatgatat      720

agtaaataag gttattaaac aagaaagaag aagcaacccg cttctcgcct cgttaacacg      780

aacgttttca ggcaaaaaat tcaaactttc gtcgcgtagc ttacgcgatt ttgaatgtgc      840

gggattgctg aaaagcagcc cgttttttta tggcctccga acgaatgagt tagcaggccg      900

cagatttgaa cagctatttt ctatcttgtt gtaacaaaat taagtggagg tggctcacca      960

ttagcaaaga catgttggta aacgatggga ttcgtgcacg tgaagtaaga ttgatcgacc     1020

aagacggtga acaattaggc gtgaagagta aaatcgatgc gcttcaaatt gctgaaaagg     1080

ctaatcttga tctagtgctt gttgctccaa cagcgaaacc gccagtagct cgtactgcag     1140


<210>  39
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to verify deletion of ActA

<400>  39
tgggatggcc aagaaattc                                                    19


<210>  40
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer to verify deletion of ActA

<400>  40
ctaccatgtc ttccgttgct tg                                                22


<210>  41
<211>  1256
<212>  DNA
<213>  Listeria monocytogenes

<400>  41
gcgccaaatc attggttgat tggtgaggat gtctgtgtgc gtgggtcgcg agatgggcga       60

ataagaagca ttaaagatcc tgacaaatat aatcaagcgg ctcatatgaa agattacgaa      120

tcgcttccac tcacagagga aggcgactgg ggcggagttc attataatag tggtatcccg      180

aataaagcag cctataatac tatcactaaa cttggaaaag aaaaaacaga acagctttat      240

tttcgcgcct taaagtacta tttaacgaaa aaatcccagt ttaccgatgc gaaaaaagcg      300

cttcaacaag cagcgaaaga tttatatggt gaagatgctt ctaaaaaagt tgctgaagct      360

tgggaagcag ttggggttaa ctgattaaca aatgttagag aaaaattaat tctccaagtg      420

atattcttaa aataattcat gaatattttt tcttatatta gctaattaag aagataacta      480

actgctaatc caatttttaa cggaacaaat tagtgaaaat gaaggccgaa ttttccttgt      540

tctaaaaagg ttgtattagc gtatcacgag gagggagtat aagtgggatt aaacagattt      600

atgcgtgcga tgatggtggt tttcattact gccaattgca ttacgattaa ccccgacgtc      660

gacccatacg acgttaattc ttgcaatgtt agctattggc gtgttctctt taggggcgtt      720

tatcaaaatt attcaattaa gaaaaaataa ttaaaaacac agaacgaaag aaaaagtgag      780

gtgaatgata tgaaattcaa aaaggtggtt ctaggtatgt gcttgatcgc aagtgttcta      840

gtctttccgg taacgataaa agcaaatgcc tgttgtgatg aatacttaca aacacccgca      900

gctccgcatg atattgacag caaattacca cataaactta gttggtccgc ggataacccg      960

acaaatactg acgtaaatac gcactattgg ctttttaaac aagcggaaaa aatactagct     1020

aaagatgtaa atcatatgcg agctaattta atgaatgaac ttaaaaaatt cgataaacaa     1080

atagctcaag gaatatatga tgcggatcat aaaaatccat attatgatac tagtacattt     1140

ttatctcatt tttataatcc tgatagagat aatacttatt tgccgggttt tgctaatgcg     1200

aaaataacag gagcaaagta tttcaatcaa tcggtgactg attaccgaga agggaa         1256


