                         SEQUENCE LISTING

<110>  Boehringer Ingelheim International GmbH
 
<120>  Inducible expression of SGLT5, methods and kits using the same

<130>  P01-2702/WO/1

<150>  11158390.2
<151>  2011-03-16

<160>  9     

<170>  PatentIn version 3.3

<210>  1
<211>  1791
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (1)..(1788)

<400>  1
atg gcc gcc aac tcc acc agc gac ctc cac act ccc ggg acg cag ctg       48
Met Ala Ala Asn Ser Thr Ser Asp Leu His Thr Pro Gly Thr Gln Leu         
1               5                   10                  15              

agc gtg gct gac atc atc gtc atc act gtg tat ttt gct ctg aat gtg       96
Ser Val Ala Asp Ile Ile Val Ile Thr Val Tyr Phe Ala Leu Asn Val         
            20                  25                  30                  

gcc gtg ggc ata tgg tcc tct tgt cgg gcc agt agg aac acg gtg aat      144
Ala Val Gly Ile Trp Ser Ser Cys Arg Ala Ser Arg Asn Thr Val Asn         
        35                  40                  45                      

ggc tac ttc ctg gca ggc cgg gac atg acg tgg tgg ccg att gga gcc      192
Gly Tyr Phe Leu Ala Gly Arg Asp Met Thr Trp Trp Pro Ile Gly Ala         
    50                  55                  60                          

tcc ctc ttc gcc agc agc gag ggc tct ggc ctc ttc att gga ctg gcg      240
Ser Leu Phe Ala Ser Ser Glu Gly Ser Gly Leu Phe Ile Gly Leu Ala         
65                  70                  75                  80          

ggc tca ggc gcg gca gga ggt ctg gcc gtg gca ggc ttc gag tgg aat      288
Gly Ser Gly Ala Ala Gly Gly Leu Ala Val Ala Gly Phe Glu Trp Asn         
                85                  90                  95              

gcc acg tac gtg ctg ctg gca ctg gca tgg gtg ttc gtg ccc atc tac      336
Ala Thr Tyr Val Leu Leu Ala Leu Ala Trp Val Phe Val Pro Ile Tyr         
            100                 105                 110                 

atc tcc tca gag atc gtc acc tta cct gag tac att cag aag cgc tac      384
Ile Ser Ser Glu Ile Val Thr Leu Pro Glu Tyr Ile Gln Lys Arg Tyr         
        115                 120                 125                     

ggg ggc cag cgg atc cgc atg tac ctg tct gtc ctg tcc ctg cta ctg      432
Gly Gly Gln Arg Ile Arg Met Tyr Leu Ser Val Leu Ser Leu Leu Leu         
    130                 135                 140                         

tct gtc ttc acc aag ata tcg ctg gac ctg tac gcg ggg gct ctg ttt      480
Ser Val Phe Thr Lys Ile Ser Leu Asp Leu Tyr Ala Gly Ala Leu Phe         
145                 150                 155                 160         

gtg cac atc tgc ctg ggc tgg aac ttc tac ctc tcc acc atc ctc acg      528
Val His Ile Cys Leu Gly Trp Asn Phe Tyr Leu Ser Thr Ile Leu Thr         
                165                 170                 175             

ctc ggc atc aca gcc ctg tac acc atc gca ggg ggc ctg gct gct gta      576
Leu Gly Ile Thr Ala Leu Tyr Thr Ile Ala Gly Gly Leu Ala Ala Val         
            180                 185                 190                 

atc tac acg gac gcc ctg cag acg ctc atc atg gtg gtg ggg gct gtc      624
Ile Tyr Thr Asp Ala Leu Gln Thr Leu Ile Met Val Val Gly Ala Val         
        195                 200                 205                     

atc ctg aca atc aaa gct ttt gac cag atc ggt ggt tac ggg cag ctg      672
Ile Leu Thr Ile Lys Ala Phe Asp Gln Ile Gly Gly Tyr Gly Gln Leu         
    210                 215                 220                         

gag gca gcc tac gcc cag gcc att ccc tcc agg acc att gcc aac acc      720
Glu Ala Ala Tyr Ala Gln Ala Ile Pro Ser Arg Thr Ile Ala Asn Thr         
225                 230                 235                 240         

acc tgc cac ctg cca cgt aca gac gcc atg cac atg ttt cga gac ccc      768
Thr Cys His Leu Pro Arg Thr Asp Ala Met His Met Phe Arg Asp Pro         
                245                 250                 255             

cac aca ggg gac ctg ccg tgg acc ggg atg acc ttt ggc ctg acc atc      816
His Thr Gly Asp Leu Pro Trp Thr Gly Met Thr Phe Gly Leu Thr Ile         
            260                 265                 270                 

atg gcc acc tgg tac tgg tgc acc gac cag gtc atc gtg cag cga tca      864
Met Ala Thr Trp Tyr Trp Cys Thr Asp Gln Val Ile Val Gln Arg Ser         
        275                 280                 285                     

ctg tca gcc cgg gac ctg aac cat gcc aag gcg ggc tcc atc ctg gcc      912
Leu Ser Ala Arg Asp Leu Asn His Ala Lys Ala Gly Ser Ile Leu Ala         
    290                 295                 300                         

agc tac ctc aag atg ctc ccc atg ggc ctg atc atc atg ccg ggc atg      960
Ser Tyr Leu Lys Met Leu Pro Met Gly Leu Ile Ile Met Pro Gly Met         
305                 310                 315                 320         

atc agc cgc gca ttg ttc cca gat gat gtg ggc tgc gtg gtg ccg tcc     1008
Ile Ser Arg Ala Leu Phe Pro Asp Asp Val Gly Cys Val Val Pro Ser         
                325                 330                 335             

gag tgc ctg cgg gcc tgc ggg gcc gag gtc ggc tgc tcc aac atc gcc     1056
Glu Cys Leu Arg Ala Cys Gly Ala Glu Val Gly Cys Ser Asn Ile Ala         
            340                 345                 350                 

tac ccc aag ctg gtc atg gaa ctg atg ccc atc ggt ctg cgg ggg ctg     1104
Tyr Pro Lys Leu Val Met Glu Leu Met Pro Ile Gly Leu Arg Gly Leu         
        355                 360                 365                     

atg atc gca gtg atg ctg gcg gcg ctc atg tcg tcg ctg acc tcc atc     1152
Met Ile Ala Val Met Leu Ala Ala Leu Met Ser Ser Leu Thr Ser Ile         
    370                 375                 380                         

ttc aac agc agc agc acc ctc ttc act atg gac atc tgg agg cgg ctg     1200
Phe Asn Ser Ser Ser Thr Leu Phe Thr Met Asp Ile Trp Arg Arg Leu         
385                 390                 395                 400         

cgt ccc cgc tcc ggc gag cgg gag ctc ctg ctg gtg gga cgg ctg gtc     1248
Arg Pro Arg Ser Gly Glu Arg Glu Leu Leu Leu Val Gly Arg Leu Val         
                405                 410                 415             

ata gtg gca ctc atc ggc gtg agt gtg gcc tgg atc ccc gtc ctg cag     1296
Ile Val Ala Leu Ile Gly Val Ser Val Ala Trp Ile Pro Val Leu Gln         
            420                 425                 430                 

gac tcc aac agc ggg caa ctc ttc atc tac atg cag tca gtg acc agc     1344
Asp Ser Asn Ser Gly Gln Leu Phe Ile Tyr Met Gln Ser Val Thr Ser         
        435                 440                 445                     

tcc ctg gcc cca cca gtg act gca gtc ttt gtc ctg ggc gtc ttc tgg     1392
Ser Leu Ala Pro Pro Val Thr Ala Val Phe Val Leu Gly Val Phe Trp         
    450                 455                 460                         

cga cgt gcc aac gag cag ggg gcc ttc tgg ggc ctg ata gca ggg ctg     1440
Arg Arg Ala Asn Glu Gln Gly Ala Phe Trp Gly Leu Ile Ala Gly Leu         
465                 470                 475                 480         

gtg gtg ggg gcc acg agg ctg gtc ctg gaa ttc ctg aac cca gcc cca     1488
Val Val Gly Ala Thr Arg Leu Val Leu Glu Phe Leu Asn Pro Ala Pro         
                485                 490                 495             

ccg tgc gga gag cca gac acg cgg cca gcc gtc ctg ggg agc atc cac     1536
Pro Cys Gly Glu Pro Asp Thr Arg Pro Ala Val Leu Gly Ser Ile His         
            500                 505                 510                 

tac ctg cac ttc gct gtc gcc ctc ttt gca ctc agt ggt gct gtt gtg     1584
Tyr Leu His Phe Ala Val Ala Leu Phe Ala Leu Ser Gly Ala Val Val         
        515                 520                 525                     

gtg gct gga agc ctg ctg acc cca ccc cca cag agt gtc cag att gag     1632
Val Ala Gly Ser Leu Leu Thr Pro Pro Pro Gln Ser Val Gln Ile Glu         
    530                 535                 540                         

aac ctt acc tgg tgg acc ctg gct cag gat gtg ccc ttg gga act aaa     1680
Asn Leu Thr Trp Trp Thr Leu Ala Gln Asp Val Pro Leu Gly Thr Lys         
545                 550                 555                 560         

gca ggt gat ggc caa aca ccc cag aaa cac gcc ttc tgg gcc cgt gtc     1728
Ala Gly Asp Gly Gln Thr Pro Gln Lys His Ala Phe Trp Ala Arg Val         
                565                 570                 575             

tgt ggc ttc aat gcc atc ctc ctc atg tgt gtc aac ata ttc ttt tat     1776
Cys Gly Phe Asn Ala Ile Leu Leu Met Cys Val Asn Ile Phe Phe Tyr         
            580                 585                 590                 

gcc tac ttc gcc tga                                                 1791
Ala Tyr Phe Ala                                                         
        595                                                             


<210>  2
<211>  596
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ala Ala Asn Ser Thr Ser Asp Leu His Thr Pro Gly Thr Gln Leu 
1               5                   10                  15      


Ser Val Ala Asp Ile Ile Val Ile Thr Val Tyr Phe Ala Leu Asn Val 
            20                  25                  30          


Ala Val Gly Ile Trp Ser Ser Cys Arg Ala Ser Arg Asn Thr Val Asn 
        35                  40                  45              


Gly Tyr Phe Leu Ala Gly Arg Asp Met Thr Trp Trp Pro Ile Gly Ala 
    50                  55                  60                  


Ser Leu Phe Ala Ser Ser Glu Gly Ser Gly Leu Phe Ile Gly Leu Ala 
65                  70                  75                  80  


Gly Ser Gly Ala Ala Gly Gly Leu Ala Val Ala Gly Phe Glu Trp Asn 
                85                  90                  95      


Ala Thr Tyr Val Leu Leu Ala Leu Ala Trp Val Phe Val Pro Ile Tyr 
            100                 105                 110         


Ile Ser Ser Glu Ile Val Thr Leu Pro Glu Tyr Ile Gln Lys Arg Tyr 
        115                 120                 125             


Gly Gly Gln Arg Ile Arg Met Tyr Leu Ser Val Leu Ser Leu Leu Leu 
    130                 135                 140                 


Ser Val Phe Thr Lys Ile Ser Leu Asp Leu Tyr Ala Gly Ala Leu Phe 
145                 150                 155                 160 


Val His Ile Cys Leu Gly Trp Asn Phe Tyr Leu Ser Thr Ile Leu Thr 
                165                 170                 175     


Leu Gly Ile Thr Ala Leu Tyr Thr Ile Ala Gly Gly Leu Ala Ala Val 
            180                 185                 190         


Ile Tyr Thr Asp Ala Leu Gln Thr Leu Ile Met Val Val Gly Ala Val 
        195                 200                 205             


Ile Leu Thr Ile Lys Ala Phe Asp Gln Ile Gly Gly Tyr Gly Gln Leu 
    210                 215                 220                 


Glu Ala Ala Tyr Ala Gln Ala Ile Pro Ser Arg Thr Ile Ala Asn Thr 
225                 230                 235                 240 


Thr Cys His Leu Pro Arg Thr Asp Ala Met His Met Phe Arg Asp Pro 
                245                 250                 255     


His Thr Gly Asp Leu Pro Trp Thr Gly Met Thr Phe Gly Leu Thr Ile 
            260                 265                 270         


Met Ala Thr Trp Tyr Trp Cys Thr Asp Gln Val Ile Val Gln Arg Ser 
        275                 280                 285             


Leu Ser Ala Arg Asp Leu Asn His Ala Lys Ala Gly Ser Ile Leu Ala 
    290                 295                 300                 


Ser Tyr Leu Lys Met Leu Pro Met Gly Leu Ile Ile Met Pro Gly Met 
305                 310                 315                 320 


Ile Ser Arg Ala Leu Phe Pro Asp Asp Val Gly Cys Val Val Pro Ser 
                325                 330                 335     


Glu Cys Leu Arg Ala Cys Gly Ala Glu Val Gly Cys Ser Asn Ile Ala 
            340                 345                 350         


Tyr Pro Lys Leu Val Met Glu Leu Met Pro Ile Gly Leu Arg Gly Leu 
        355                 360                 365             


Met Ile Ala Val Met Leu Ala Ala Leu Met Ser Ser Leu Thr Ser Ile 
    370                 375                 380                 


Phe Asn Ser Ser Ser Thr Leu Phe Thr Met Asp Ile Trp Arg Arg Leu 
385                 390                 395                 400 


Arg Pro Arg Ser Gly Glu Arg Glu Leu Leu Leu Val Gly Arg Leu Val 
                405                 410                 415     


Ile Val Ala Leu Ile Gly Val Ser Val Ala Trp Ile Pro Val Leu Gln 
            420                 425                 430         


Asp Ser Asn Ser Gly Gln Leu Phe Ile Tyr Met Gln Ser Val Thr Ser 
        435                 440                 445             


Ser Leu Ala Pro Pro Val Thr Ala Val Phe Val Leu Gly Val Phe Trp 
    450                 455                 460                 


Arg Arg Ala Asn Glu Gln Gly Ala Phe Trp Gly Leu Ile Ala Gly Leu 
465                 470                 475                 480 


Val Val Gly Ala Thr Arg Leu Val Leu Glu Phe Leu Asn Pro Ala Pro 
                485                 490                 495     


Pro Cys Gly Glu Pro Asp Thr Arg Pro Ala Val Leu Gly Ser Ile His 
            500                 505                 510         


Tyr Leu His Phe Ala Val Ala Leu Phe Ala Leu Ser Gly Ala Val Val 
        515                 520                 525             


Val Ala Gly Ser Leu Leu Thr Pro Pro Pro Gln Ser Val Gln Ile Glu 
    530                 535                 540                 


Asn Leu Thr Trp Trp Thr Leu Ala Gln Asp Val Pro Leu Gly Thr Lys 
545                 550                 555                 560 


Ala Gly Asp Gly Gln Thr Pro Gln Lys His Ala Phe Trp Ala Arg Val 
                565                 570                 575     


Cys Gly Phe Asn Ala Ile Leu Leu Met Cys Val Asn Ile Phe Phe Tyr 
            580                 585                 590         


Ala Tyr Phe Ala 
        595     


<210>  3
<211>  2091
<212>  DNA
<213>  Homo sapiens

<400>  3
agtccctcgg gctcatacct agtgcctgcg gcaggacagc catggccgcc aactccacca     60

gcgacctcca cactcccggg acgcagctga gcgtggctga catcatcgtc atcactgtgt    120

attttgctct gaatgtggcc gtgggcatat ggtcctcttg tcgggccagt aggaacacgg    180

tgaatggcta cttcctggca ggccgggaca tgacgtggtg gccgattgga gcctccctct    240

tcgccagcag cgagggctct ggcctcttca ttggactggc gggctcaggc gcggcaggag    300

gtctggccgt ggcaggcttc gagtggaatg ccacgtacgt gctgctggca ctggcatggg    360

tgttcgtgcc catctacatc tcctcagaga tcgtcacctt acctgagtac attcagaagc    420

gctacggggg ccagcggatc cgcatgtacc tgtctgtcct gtccctgcta ctgtctgtct    480

tcaccaagat atcgctggac ctgtacgcgg gggctctgtt tgtgcacatc tgcctgggct    540

ggaacttcta cctctccacc atcctcacgc tcggcatcac agccctgtac accatcgcag    600

ggggcctggc tgctgtaatc tacacggacg ccctgcagac gctcatcatg gtggtggggg    660

ctgtcatcct gacaatcaaa gcttttgacc agatcggtgg ttacgggcag ctggaggcag    720

cctacgccca ggccattccc tccaggacca ttgccaacac cacctgccac ctgccacgta    780

cagacgccat gcacatgttt cgagaccccc acacagggga cctgccgtgg accgggatga    840

cctttggcct gaccatcatg gccacctggt actggtgcac cgaccaggtc atcgtgcagc    900

gatcactgtc agcccgggac ctgaaccatg ccaaggcggg ctccatcctg gccagctacc    960

tcaagatgct ccccatgggc ctgatcatca tgccgggcat gatcagccgc gcattgttcc   1020

cagatgatgt gggctgcgtg gtgccgtccg agtgcctgcg ggcctgcggg gccgaggtcg   1080

gctgctccaa catcgcctac cccaagctgg tcatggaact gatgcccatc ggtctgcggg   1140

ggctgatgat cgcagtgatg ctggcggcgc tcatgtcgtc gctgacctcc atcttcaaca   1200

gcagcagcac cctcttcact atggacatct ggaggcggct gcgtccccgc tccggcgagc   1260

gggagctcct gctggtggga cggctggtca tagtggcact catcggcgtg agtgtggcct   1320

ggatccccgt cctgcaggac tccaacagcg ggcaactctt catctacatg cagtcagtga   1380

ccagctccct ggccccacca gtgactgcag tctttgtcct gggcgtcttc tggcgacgtg   1440

ccaacgagca gggggccttc tggggcctga tagcagggct ggtggtgggg gccacgaggc   1500

tggtcctgga attcctgaac ccagccccac cgtgcggaga gccagacacg cggccagccg   1560

tcctggggag catccactac ctgcacttcg ctgtcgccct ctttgcactc agtggtgctg   1620

ttgtggtggc tggaagcctg ctgaccccac ccccacagag tgtccagatt gagaacctta   1680

cctggtggac cctggctcag gatgtgccct tgggaactaa agcaggtgat ggccaaacac   1740

cccagaaaca cgccttctgg gcccgtgtct gtggcttcaa tgccatcctc ctcatgtgtg   1800

tcaacatatt cttttatgcc tacttcgcct gacactgcca tcctggacag aaaggcagga   1860

gctctgagtc ctcaggtcca cccatttccc tcatggggat cccgaggccc caagaggggc   1920

agattcccct cacagctgca cagcagctcg gtgcccaaga actggccaag ccagcaaagc   1980

gggagccctg aaaaattagg ggggaaatgg gagaaaataa tgtgacattt caaaaacagc   2040

accaaagcag tcagcattgg aaggaaaatt agatttctga cggacatcct g            2091


<210>  4
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  PCR primer

<400>  4
caccgccatg gccgccaact cca                                             23


<210>  5
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  PCR primer

<400>  5
tcaggcgaag taggcataaa agaatatgtt gac                                  33


<210>  6
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  PCR primer

<400>  6
gatgaccttt ggcctgacca t                                               21


<210>  7
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  PCR primer

<400>  7
gtcccgggct gacagtgat                                                  19


<210>  8
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  PCR primer

<400>  8
tggcatggac tgtggtca                                                   18


<210>  9
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  PCR primer

<400>  9
gcaccaccaa ctgcttag                                                   18


