                         SEQUENCE LISTING

<110>  SANOFI
 
<120>  Single nucleotide polymorphisms in the promoter of VEGFA gene and
        their use as predictive markers for anti-VEGF treatments

<130>  FR2011-011 PCT

<160>  6     

<170>  PatentIn version 3.3

<210>  1
<211>  2961
<212>  DNA
<213>  Homo sapiens


<220>
<221>  promoter
<222>  (1)..(2961)
<223>  Promoter region of VEGFA gene (wild type allele)

<400>  1
gctcccaagg tgaggccctc caagcggggc cgagtttgcc cctcaactgg gagccagcat     60

gacctctgtg tgggctgctc tctgcttcac tgccccttcc cccaatctgc taggtgaccc    120

tgggcccctt tgtgccctct ctgggccttc ggaggattct ttggggagac agtctgctct    180

gacgcccctt cccctgcagc aagcagcctg gggagggagg tgaggataag tgaagtcaag    240

ttgttcaggg ggctaagccc atggaaggga agatgccaca gagatacatg tggtcttgtg    300

attgttgttt tgtgcttttt cccctttttt gaaagctcag gtgactaggt gacttgagct    360

tttaatttgg tgacaatgtg ggcactggct gagtccttaa gagtacattg ttgtaaatgc    420

cggtgacaac acactggggc atgggatcca gagttaaccc ctccaggtca cagccaggtt    480

atatctccac aatgaagggg ggaggtgggc catactttct cgccctaatg aaggtagctc    540

aaaaacccct aggccaggtt gtaatcctag ccttatataa aaggaattct gtgccctcac    600

tcccctggat ccctgggcaa agccccagag ggaaacacaa acaggttgtt gtaacacacc    660

ttgctgggta ccaccatgga ggacagttgg cttatggggg tggggggtgc ctggggccac    720

ggagtgactg gtgatggcta tccctccttg gaacccctcc agcctcctct tagcttcaga    780

tttgtttatt tgttttttac taagacctgc tctttcaggt ctgttggctc ttttaggggc    840

tgaagaaggc cgagttgaga agggatgcaa gggagggggc cagaatgagc ccttagggct    900

cagagcctcc atcctgcccc aagatgtcta cagcttgtgc tcctggggtg ctagaggcgc    960

acaaggagga aagttagtgg cttcccttcc atatcccgtt catcagccta gagcatggag   1020

cccaggtgag gaggcctgcc tgggaggggg ccctgagcca ggaaataaac atttactaac   1080

tgtacaaaga ccttgtccct gctgctgggg agcctgccaa gtggtggaga caggactagt   1140

gcacgaatga tggaaaggga gggttggggt gggtgggagc cagccctttt cctcataagg   1200

gccttaggac accataccga tggaactggg ggtactgggg aggtaaccta gcacctccac   1260

caaaccacag caacatgtgc tgaggatggg gctgactagg taagctccct ggagcgtttt   1320

ggttaaattg agggaaattg ctgcattccc attctcagtc catgcctcca cagaggctat   1380

gccagctgta ggccagaccc tggcaagatc tgggtggata atcagactga ctggtcccac   1440

tcttcccaca ggcctcagag ccccaacttt gttccctggg gcagcctgga aatagccagg   1500

tcagaaacca gctaggaatt tttccaagct gcttcctata tgcaagaatg ggatggggcc   1560

tttgggagca cttagggaag atgtggagag ttggaggaaa agggggcttg gaggtaaggg   1620

aggggactgg gggaaggata ggggagaagc tgtgagcctg gagaagtagc caagggatcc   1680

tgagggaatg ggggagctga gacgaaaccc ccatttctat tcagaagatg agctatgagt   1740

ctgggcttgg gctgatagaa gccttggccc ctggcctggt gggagctctg ggcagctggc   1800

ctacagacgt tccttagtgc tggcgggtag gtttgaatca tcacgcaggc cctggcctcc   1860

acccgccccc accagccccc tggcctcagt tccctggcaa catctggggt tgggggggca   1920

gcaggaacaa gggcctctgt ctgcccagct gcctccccct ttgggttttg ccagactcca   1980

cagtgcatac gtgggctcca acaggtcctc ttccctccca gtcactgact aaccccggaa   2040

ccacacagct tcccgttctc agctccacaa acttggtgcc aaattcttct cccctgggaa   2100

gcatccctgg acacttccca aaggacccca gtcactccag cctgttggct gccgctcact   2160

ttgatgtctg caggccagat gagggctcca gatggcacat tgtcagaggg acacactgtg   2220

gcccctgtgc ccagccctgg gctctctgta catgaagcaa ctccagtccc aaatatgtag   2280

ctgtttggga ggtcagaaat agggggtcca ggagcaaact ccccccaccc cctttccaaa   2340

gcccattccc tctttagcca gagccggggt gtgcagacgg cagtcactag ggggcgctcg   2400

gccaccacag ggaagctggg tgaatggagc gagcagcgtc ttcgagagtg aggacgtgtg   2460

tgtctgtgtg ggtgagtgag tgtgtgcgtg tggggttgag ggcgttggag cggggagaag   2520

gccaggggtc actccaggat tccaatagat ctgtgtgtcc ctctccccac ccgtccctgt   2580

ccggctctcc gccttcccct gcccccttca atattcctag caaagaggga acggctctca   2640

ggccctgtcc gcacgtaacc tcactttcct gctccctcct cgccaatgcc ccgcgggcgc   2700

gtgtctctgg acagagtttc cgggggcgga tgggtaattt tcaggctgtg aaccttggtg   2760

ggggtcgagc ttccccttca ttgcggcggg ctgcgggcca ggcttcactg agcgtccgca   2820

gagcccgggc ccgagccgcg tgtggaaggg ctgaggctcg cctgtccccg ccccccgggg   2880

cgggccgggg gcggggtccc ggcggggcgg agccatgcgc cccccccttt tttttttaaa   2940

agtcggctgg tagcggggag g                                             2961


<210>  2
<211>  2943
<212>  DNA
<213>  Homo sapiens


<220>
<221>  promoter
<222>  (1)..(2943)
<223>  promoter of VEGFA gene including the polymorphisms-2578C 
       (position 1406), -2549del (position 1434), -1498T (position 2485)
       and -1190G (position 2793)

<400>  2
gctcccaagg tgaggccctc caagcggggc cgagtttgcc cctcaactgg gagccagcat     60

gacctctgtg tgggctgctc tctgcttcac tgccccttcc cccaatctgc taggtgaccc    120

tgggcccctt tgtgccctct ctgggccttc ggaggattct ttggggagac agtctgctct    180

gacgcccctt cccctgcagc aagcagcctg gggagggagg tgaggataag tgaagtcaag    240

ttgttcaggg ggctaagccc atggaaggga agatgccaca gagatacatg tggtcttgtg    300

attgttgttt tgtgcttttt cccctttttt gaaagctcag gtgactaggt gacttgagct    360

tttaatttgg tgacaatgtg ggcactggct gagtccttaa gagtacattg ttgtaaatgc    420

cggtgacaac acactggggc atgggatcca gagttaaccc ctccaggtca cagccaggtt    480

atatctccac aatgaagggg ggaggtgggc catactttct cgccctaatg aaggtagctc    540

aaaaacccct aggccaggtt gtaatcctag ccttatataa aaggaattct gtgccctcac    600

tcccctggat ccctgggcaa agccccagag ggaaacacaa acaggttgtt gtaacacacc    660

ttgctgggta ccaccatgga ggacagttgg cttatggggg tggggggtgc ctggggccac    720

ggagtgactg gtgatggcta tccctccttg gaacccctcc agcctcctct tagcttcaga    780

tttgtttatt tgttttttac taagacctgc tctttcaggt ctgttggctc ttttaggggc    840

tgaagaaggc cgagttgaga agggatgcaa gggagggggc cagaatgagc ccttagggct    900

cagagcctcc atcctgcccc aagatgtcta cagcttgtgc tcctggggtg ctagaggcgc    960

acaaggagga aagttagtgg cttcccttcc atatcccgtt catcagccta gagcatggag   1020

cccaggtgag gaggcctgcc tgggaggggg ccctgagcca ggaaataaac atttactaac   1080

tgtacaaaga ccttgtccct gctgctgggg agcctgccaa gtggtggaga caggactagt   1140

gcacgaatga tggaaaggga gggttggggt gggtgggagc cagccctttt cctcataagg   1200

gccttaggac accataccga tggaactggg ggtactgggg aggtaaccta gcacctccac   1260

caaaccacag caacatgtgc tgaggatggg gctgactagg taagctccct ggagcgtttt   1320

ggttaaattg agggaaattg ctgcattccc attctcagtc catgcctcca cagaggctat   1380

gccagctgta ggccagaccc tggcacgatc tgggtggata atcagactga ctggcctcag   1440

agccccaact ttgttccctg gggcagcctg gaaatagcca ggtcagaaac cagctaggaa   1500

tttttccaag ctgcttccta tatgcaagaa tgggatgggg cctttgggag cacttaggga   1560

agatgtggag agttggagga aaagggggct tggaggtaag ggaggggact gggggaagga   1620

taggggagaa gctgtgagcc tggagaagta gccaagggat cctgagggaa tgggggagct   1680

gagacgaaac ccccatttct attcagaaga tgagctatga gtctgggctt gggctgatag   1740

aagccttggc ccctggcctg gtgggagctc tgggcagctg gcctacagac gttccttagt   1800

gctggcgggt aggtttgaat catcacgcag gccctggcct ccacccgccc ccaccagccc   1860

cctggcctca gttccctggc aacatctggg gttggggggg cagcaggaac aagggcctct   1920

gtctgcccag ctgcctcccc ctttgggttt tgccagactc cacagtgcat acgtgggctc   1980

caacaggtcc tcttccctcc cagtcactga ctaaccccgg aaccacacag cttcccgttc   2040

tcagctccac aaacttggtg ccaaattctt ctcccctggg aagcatccct ggacacttcc   2100

caaaggaccc cagtcactcc agcctgttgg ctgccgctca ctttgatgtc tgcaggccag   2160

atgagggctc cagatggcac attgtcagag ggacacactg tggcccctgt gcccagccct   2220

gggctctctg tacatgaagc aactccagtc ccaaatatgt agctgtttgg gaggtcagaa   2280

atagggggtc caggagcaaa ctccccccac cccctttcca aagcccattc cctctttagc   2340

cagagccggg gtgtgcagac ggcagtcact agggggcgct cggccaccac agggaagctg   2400

ggtgaatgga gcgagcagcg tcttcgagag tgaggacgtg tgtgtctgtg tgggtgagtg   2460

agtgtgtgcg tgtggggttg agggtgttgg agcggggaga aggccagggg tcactccagg   2520

attccaatag atctgtgtgt ccctctcccc acccgtccct gtccggctct ccgccttccc   2580

ctgccccctt caatattcct agcaaagagg gaacggctct caggccctgt ccgcacgtaa   2640

cctcactttc ctgctccctc ctcgccaatg ccccgcgggc gcgtgtctct ggacagagtt   2700

tccgggggcg gatgggtaat tttcaggctg tgaaccttgg tgggggtcga gcttcccctt   2760

cattgcggcg ggctgcgggc caggcttcac tgggcgtccg cagagcccgg gcccgagccg   2820

cgtgtggaag ggctgaggct cgcctgtccc cgccccccgg ggcgggccgg gggcggggtc   2880

ccggcggggc ggagccatgc gcccccccct ttttttttta aaagtcggct ggtagcgggg   2940

agg                                                                 2943


<210>  3
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  Primer-bind


<220>
<221>  primer_bind
<222>  (1)..(16)

<400>  3
ctggggagcc aagtgg                                                     16


<210>  4
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Primer-bind


<220>
<221>  primer_bind
<222>  (1)..(25)
<223>  Reverse PCR primer to amplify the promoter region of VEGFA and 
       detect -2578C and -2549del alleles.

<400>  4
ccagcactaa ggaacgtctg taggc                                           25


<210>  5
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  Primer-bind


<220>
<221>  primer_bind
<222>  (1)..(17)
<223>  forward PCR primer to amplify the promoter region of VEGFA and 
       detect -1498T and -1190G alleles.

<400>  5
gcgagcgtct tcgagag                                                    17


<210>  6
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Primer-bind


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  Reverse PCR primer to amplify the promoter region of VEGFA and 
       detect -1498Tand -1190G alleles.

<400>  6
ccggtccacc taaccgctgc                                                 20


