                               SEQUENCE LISTING

<110> NEW YORK UNIVERSITY
 
<120> METHODS AND COMPOSITIONS FOR MODULATING TNF/TNFR SIGNALING

<130> 102338-0102

<140> PCT/US12/28458
<141> 2012-03-09

<150> 61/451,190
<151> 2011-03-10

<160> 28    

<170> PatentIn version 3.5

<210> 1
<211> 593
<212> PRT
<213> Homo sapiens

<400> 1
Met Trp Thr Leu Val Ser Trp Val Ala Leu Thr Ala Gly Leu Val Ala 
1               5                   10                  15      


Gly Thr Arg Cys Pro Asp Gly Gln Phe Cys Pro Val Ala Cys Cys Leu 
            20                  25                  30          


Asp Pro Gly Gly Ala Ser Tyr Ser Cys Cys Arg Pro Leu Leu Asp Lys 
        35                  40                  45              


Trp Pro Thr Thr Leu Ser Arg His Leu Gly Gly Pro Cys Gln Val Asp 
    50                  55                  60                  


Ala His Cys Ser Ala Gly His Ser Cys Ile Phe Thr Val Ser Gly Thr 
65                  70                  75                  80  


Ser Ser Cys Cys Pro Phe Pro Glu Ala Val Ala Cys Gly Asp Gly His 
                85                  90                  95      


His Cys Cys Pro Arg Gly Phe His Cys Ser Ala Asp Gly Arg Ser Cys 
            100                 105                 110         


Phe Gln Arg Ser Gly Asn Asn Ser Val Gly Ala Ile Gln Cys Pro Asp 
        115                 120                 125             


Ser Gln Phe Glu Cys Pro Asp Phe Ser Thr Cys Cys Val Met Val Asp 
    130                 135                 140                 


Gly Ser Trp Gly Cys Cys Pro Met Pro Gln Ala Ser Cys Cys Glu Asp 
145                 150                 155                 160 


Arg Val His Cys Cys Pro His Gly Ala Phe Cys Asp Leu Val His Thr 
                165                 170                 175     


Arg Cys Ile Thr Pro Thr Gly Thr His Pro Leu Ala Lys Lys Leu Pro 
            180                 185                 190         


Ala Gln Arg Thr Asn Arg Ala Val Ala Leu Ser Ser Ser Val Met Cys 
        195                 200                 205             


Pro Asp Ala Arg Ser Arg Cys Pro Asp Gly Ser Thr Cys Cys Glu Leu 
    210                 215                 220                 


Pro Ser Gly Lys Tyr Gly Cys Cys Pro Met Pro Asn Ala Thr Cys Cys 
225                 230                 235                 240 


Ser Asp His Leu His Cys Cys Pro Gln Asp Thr Val Cys Asp Leu Ile 
                245                 250                 255     


Gln Ser Lys Cys Leu Ser Lys Glu Asn Ala Thr Thr Asp Leu Leu Thr 
            260                 265                 270         


Lys Leu Pro Ala His Thr Val Gly Asp Val Lys Cys Asp Met Glu Val 
        275                 280                 285             


Ser Cys Pro Asp Gly Tyr Thr Cys Cys Arg Leu Gln Ser Gly Ala Trp 
    290                 295                 300                 


Gly Cys Cys Pro Phe Thr Gln Ala Val Cys Cys Glu Asp His Ile His 
305                 310                 315                 320 


Cys Cys Pro Ala Gly Phe Thr Cys Asp Thr Gln Lys Gly Thr Cys Glu 
                325                 330                 335     


Gln Gly Pro His Gln Val Pro Trp Met Glu Lys Ala Pro Ala His Leu 
            340                 345                 350         


Ser Leu Pro Asp Pro Gln Ala Leu Lys Arg Asp Val Pro Cys Asp Asn 
        355                 360                 365             


Val Ser Ser Cys Pro Ser Ser Asp Thr Cys Cys Gln Leu Thr Ser Gly 
    370                 375                 380                 


Glu Trp Gly Cys Cys Pro Ile Pro Glu Ala Val Cys Cys Ser Asp His 
385                 390                 395                 400 


Gln His Cys Cys Pro Gln Gly Tyr Thr Cys Val Ala Glu Gly Gln Cys 
                405                 410                 415     


Gln Arg Gly Ser Glu Ile Val Ala Gly Leu Glu Lys Met Pro Ala Arg 
            420                 425                 430         


Arg Ala Ser Leu Ser His Pro Arg Asp Ile Gly Cys Asp Gln His Thr 
        435                 440                 445             


Ser Cys Pro Val Gly Gln Thr Cys Cys Pro Ser Leu Gly Gly Ser Trp 
    450                 455                 460                 


Ala Cys Cys Gln Leu Pro His Ala Val Cys Cys Glu Asp Arg Gln His 
465                 470                 475                 480 


Cys Cys Pro Ala Gly Tyr Thr Cys Asn Val Lys Ala Arg Ser Cys Glu 
                485                 490                 495     


Lys Glu Val Val Ser Ala Gln Pro Ala Thr Phe Leu Ala Arg Ser Pro 
            500                 505                 510         


His Val Gly Val Lys Asp Val Glu Cys Gly Glu Gly His Phe Cys His 
        515                 520                 525             


Asp Asn Gln Thr Cys Cys Arg Asp Asn Arg Gln Gly Trp Ala Cys Cys 
    530                 535                 540                 


Pro Tyr Arg Gln Gly Val Cys Cys Ala Asp Arg Arg His Cys Cys Pro 
545                 550                 555                 560 


Ala Gly Phe Arg Cys Ala Ala Arg Gly Thr Lys Cys Leu Arg Arg Glu 
                565                 570                 575     


Ala Pro Arg Trp Asp Ala Pro Leu Arg Asp Pro Ala Leu Arg Gln Leu 
            580                 585                 590         


Leu 
    


<210> 2
<211> 158
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 2
Pro Gln Ala Ser Cys Cys Glu Asp Arg Val His Cys Cys Pro His Gly 
1               5                   10                  15      


Ala Phe Cys Asp Leu Val His Thr Arg Cys Ile Thr Pro Thr Gly Thr 
            20                  25                  30          


His Pro Leu Ala Lys Lys Leu Pro Ala Gln Arg Thr Asn Arg Ala Val 
        35                  40                  45              


Ala Leu Ser Ser Ser Ala Ser Ser Lys Glu Asn Ala Thr Thr Asp Leu 
    50                  55                  60                  


Leu Thr Lys Leu Pro Ala His Thr Val Gly Asp Val Lys Cys Asp Met 
65                  70                  75                  80  


Glu Val Ser Cys Pro Asp Gly Tyr Thr Cys Cys Arg Leu Gln Ser Gly 
                85                  90                  95      


Ala Trp Pro Trp Cys Glu Gln Gly Pro His Gln Val Pro Trp Met Glu 
            100                 105                 110         


Lys Ala Pro Ala His Leu Ser Leu Pro Asp Pro Gln Ala Leu Lys Arg 
        115                 120                 125             


Asp Val Pro Cys Asp Asn Val Ser Ser Cys Pro Ser Ser Asp Thr Cys 
    130                 135                 140                 


Cys Gln Leu Thr Ser Gly Glu Trp Gly Cys Cys Pro Ile Pro 
145                 150                 155             


<210> 3
<211> 157
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 3
Gly Ile Leu Pro Gln Ala Ser Cys Cys Glu Asp Arg Val His Cys Cys 
1               5                   10                  15      


Pro His Gly Ala Phe Cys Asp Leu Val His Thr Arg Cys Ile Thr Pro 
            20                  25                  30          


Thr Gly Thr His Pro Leu Ala Lys Lys Leu Pro Ala Gln Arg Thr Asn 
        35                  40                  45              


Arg Ala Val Ala Leu Ser Ser Ser Ser Lys Glu Asn Ala Thr Thr Asp 
    50                  55                  60                  


Leu Leu Thr Lys Leu Pro Ala His Thr Val Gly Asp Val Lys Cys Asp 
65                  70                  75                  80  


Met Glu Val Ser Cys Pro Asp Gly Tyr Thr Cys Cys Arg Leu Gln Ser 
                85                  90                  95      


Gly Ala Trp Cys Glu Gln Gly Pro His Gln Val Pro Trp Met Glu Lys 
            100                 105                 110         


Ala Pro Ala His Leu Ser Leu Pro Asp Pro Gln Ala Leu Lys Arg Asp 
        115                 120                 125             


Val Pro Cys Asp Asn Val Ser Ser Cys Pro Ser Ser Asp Thr Cys Cys 
    130                 135                 140                 


Gln Leu Thr Ser Gly Glu Trp Gly Cys Cys Pro Ile Pro 
145                 150                 155         


<210> 4
<211> 154
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 4
Pro Gln Ala Ser Cys Cys Glu Asp Arg Val His Cys Cys Pro His Gly 
1               5                   10                  15      


Ala Phe Cys Asp Leu Val His Thr Arg Cys Ile Thr Pro Thr Gly Thr 
            20                  25                  30          


His Pro Leu Ala Lys Lys Leu Pro Ala Gln Arg Thr Asn Arg Ala Val 
        35                  40                  45              


Ala Leu Ser Ser Ser Ser Lys Glu Asn Ala Thr Thr Asp Leu Leu Thr 
    50                  55                  60                  


Lys Leu Pro Ala His Thr Val Gly Asp Val Lys Cys Asp Met Glu Val 
65                  70                  75                  80  


Ser Cys Pro Asp Gly Tyr Thr Cys Cys Arg Leu Gln Ser Gly Ala Trp 
                85                  90                  95      


Cys Glu Gln Gly Pro His Gln Val Pro Trp Met Glu Lys Ala Pro Ala 
            100                 105                 110         


His Leu Ser Leu Pro Asp Pro Gln Ala Leu Lys Arg Asp Val Pro Cys 
        115                 120                 125             


Asp Asn Val Ser Ser Cys Pro Ser Ser Asp Thr Cys Cys Gln Leu Thr 
    130                 135                 140                 


Ser Gly Glu Trp Gly Cys Cys Pro Ile Pro 
145                 150                 


<210> 5
<211> 155
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 5
Met Pro Gln Ala Ser Cys Cys Glu Asp Arg Val His Cys Cys Pro His 
1               5                   10                  15      


Gly Ala Phe Cys Asp Leu Val His Thr Arg Cys Ile Thr Pro Thr Gly 
            20                  25                  30          


Thr His Pro Leu Ala Lys Lys Leu Pro Ala Gln Arg Thr Asn Arg Ala 
        35                  40                  45              


Val Ala Leu Ser Ser Ser Ser Lys Glu Asn Ala Thr Thr Asp Leu Leu 
    50                  55                  60                  


Thr Lys Leu Pro Ala His Thr Val Gly Asp Val Lys Cys Asp Met Glu 
65                  70                  75                  80  


Val Ser Cys Pro Asp Gly Tyr Thr Cys Cys Arg Leu Gln Ser Gly Ala 
                85                  90                  95      


Trp Cys Glu Gln Gly Pro His Gln Val Pro Trp Met Glu Lys Ala Pro 
            100                 105                 110         


Ala His Leu Ser Leu Pro Asp Pro Gln Ala Leu Lys Arg Asp Val Pro 
        115                 120                 125             


Cys Asp Asn Val Ser Ser Cys Pro Ser Ser Asp Thr Cys Cys Gln Leu 
    130                 135                 140                 


Thr Ser Gly Glu Trp Gly Cys Cys Pro Ile Pro 
145                 150                 155 


<210> 6
<211> 156
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 6
Met Leu Pro Gln Ala Ser Cys Cys Glu Asp Arg Val His Cys Cys Pro 
1               5                   10                  15      


His Gly Ala Phe Cys Asp Leu Val His Thr Arg Cys Ile Thr Pro Thr 
            20                  25                  30          


Gly Thr His Pro Leu Ala Lys Lys Leu Pro Ala Gln Arg Thr Asn Arg 
        35                  40                  45              


Ala Val Ala Leu Ser Ser Ser Ser Lys Glu Asn Ala Thr Thr Asp Leu 
    50                  55                  60                  


Leu Thr Lys Leu Pro Ala His Thr Val Gly Asp Val Lys Cys Asp Met 
65                  70                  75                  80  


Glu Val Ser Cys Pro Asp Gly Tyr Thr Cys Cys Arg Leu Gln Ser Gly 
                85                  90                  95      


Ala Trp Cys Glu Gln Gly Pro His Gln Val Pro Trp Met Glu Lys Ala 
            100                 105                 110         


Pro Ala His Leu Ser Leu Pro Asp Pro Gln Ala Leu Lys Arg Asp Val 
        115                 120                 125             


Pro Cys Asp Asn Val Ser Ser Cys Pro Ser Ser Asp Thr Cys Cys Gln 
    130                 135                 140                 


Leu Thr Ser Gly Glu Trp Gly Cys Cys Pro Ile Pro 
145                 150                 155     


<210> 7
<211> 157
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 7
Met Ile Leu Pro Gln Ala Ser Cys Cys Glu Asp Arg Val His Cys Cys 
1               5                   10                  15      


Pro His Gly Ala Phe Cys Asp Leu Val His Thr Arg Cys Ile Thr Pro 
            20                  25                  30          


Thr Gly Thr His Pro Leu Ala Lys Lys Leu Pro Ala Gln Arg Thr Asn 
        35                  40                  45              


Arg Ala Val Ala Leu Ser Ser Ser Ser Lys Glu Asn Ala Thr Thr Asp 
    50                  55                  60                  


Leu Leu Thr Lys Leu Pro Ala His Thr Val Gly Asp Val Lys Cys Asp 
65                  70                  75                  80  


Met Glu Val Ser Cys Pro Asp Gly Tyr Thr Cys Cys Arg Leu Gln Ser 
                85                  90                  95      


Gly Ala Trp Cys Glu Gln Gly Pro His Gln Val Pro Trp Met Glu Lys 
            100                 105                 110         


Ala Pro Ala His Leu Ser Leu Pro Asp Pro Gln Ala Leu Lys Arg Asp 
        115                 120                 125             


Val Pro Cys Asp Asn Val Ser Ser Cys Pro Ser Ser Asp Thr Cys Cys 
    130                 135                 140                 


Gln Leu Thr Ser Gly Glu Trp Gly Cys Cys Pro Ile Pro 
145                 150                 155         


<210> 8
<211> 158
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 8
Met Gly Ile Leu Pro Gln Ala Ser Cys Cys Glu Asp Arg Val His Cys 
1               5                   10                  15      


Cys Pro His Gly Ala Phe Cys Asp Leu Val His Thr Arg Cys Ile Thr 
            20                  25                  30          


Pro Thr Gly Thr His Pro Leu Ala Lys Lys Leu Pro Ala Gln Arg Thr 
        35                  40                  45              


Asn Arg Ala Val Ala Leu Ser Ser Ser Ser Lys Glu Asn Ala Thr Thr 
    50                  55                  60                  


Asp Leu Leu Thr Lys Leu Pro Ala His Thr Val Gly Asp Val Lys Cys 
65                  70                  75                  80  


Asp Met Glu Val Ser Cys Pro Asp Gly Tyr Thr Cys Cys Arg Leu Gln 
                85                  90                  95      


Ser Gly Ala Trp Cys Glu Gln Gly Pro His Gln Val Pro Trp Met Glu 
            100                 105                 110         


Lys Ala Pro Ala His Leu Ser Leu Pro Asp Pro Gln Ala Leu Lys Arg 
        115                 120                 125             


Asp Val Pro Cys Asp Asn Val Ser Ser Cys Pro Ser Ser Asp Thr Cys 
    130                 135                 140                 


Cys Gln Leu Thr Ser Gly Glu Trp Gly Cys Cys Pro Ile Pro 
145                 150                 155             


<210> 9
<211> 219
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 9
Ile Gln Cys Pro Asp Ser Gln Phe Glu Cys Pro Asp Phe Ser Thr Cys 
1               5                   10                  15      


Cys Val Met Val Asp Gly Ser Trp Gly Cys Cys Pro Met Pro Gln Ala 
            20                  25                  30          


Ser Cys Cys Glu Asp Arg Val His Cys Cys Pro His Gly Ala Phe Cys 
        35                  40                  45              


Asp Leu Val His Thr Arg Cys Ile Thr Pro Thr Gly Thr His Pro Ser 
    50                  55                  60                  


Lys Glu Asn Ala Thr Thr Asp Leu Leu Thr Lys Leu Pro Ala His Thr 
65                  70                  75                  80  


Val Gly Asp Val Lys Cys Asp Met Glu Val Ser Cys Pro Asp Gly Tyr 
                85                  90                  95      


Thr Cys Cys Arg Leu Gln Ser Gly Ala Trp Gly Cys Cys Pro Phe Thr 
            100                 105                 110         


Gln Ala Val Cys Cys Glu Asp His Ile His Cys Cys Pro Ala Gly Phe 
        115                 120                 125             


Thr Cys Asp Thr Gln Lys Gly Thr Cys Glu Gln Gly Pro His Gln Val 
    130                 135                 140                 


Pro Trp Met Glu Lys Ala Pro Ala His Leu Ser Leu Pro Asp Pro Gln 
145                 150                 155                 160 


Ala Leu Lys Arg Asp Val Pro Cys Asp Asn Val Ser Ser Cys Pro Ser 
                165                 170                 175     


Ser Asp Thr Cys Cys Gln Leu Thr Ser Gly Glu Trp Gly Cys Cys Pro 
            180                 185                 190         


Ile Pro Glu Ala Val Cys Cys Ser Asp His Gln His Cys Cys Pro Gln 
        195                 200                 205             


Gly Tyr Thr Cys Val Ala Glu Gly Gln Cys Gln 
    210                 215                 


<210> 10
<211> 222
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 10
Gly Ile Leu Ile Gln Cys Pro Asp Ser Gln Phe Glu Cys Pro Asp Phe 
1               5                   10                  15      


Ser Thr Cys Cys Val Met Val Asp Gly Ser Trp Gly Cys Cys Pro Met 
            20                  25                  30          


Pro Gln Ala Ser Cys Cys Glu Asp Arg Val His Cys Cys Pro His Gly 
        35                  40                  45              


Ala Phe Cys Asp Leu Val His Thr Arg Cys Ile Thr Pro Thr Gly Thr 
    50                  55                  60                  


His Pro Ser Lys Glu Asn Ala Thr Thr Asp Leu Leu Thr Lys Leu Pro 
65                  70                  75                  80  


Ala His Thr Val Gly Asp Val Lys Cys Asp Met Glu Val Ser Cys Pro 
                85                  90                  95      


Asp Gly Tyr Thr Cys Cys Arg Leu Gln Ser Gly Ala Trp Gly Cys Cys 
            100                 105                 110         


Pro Phe Thr Gln Ala Val Cys Cys Glu Asp His Ile His Cys Cys Pro 
        115                 120                 125             


Ala Gly Phe Thr Cys Asp Thr Gln Lys Gly Thr Cys Glu Gln Gly Pro 
    130                 135                 140                 


His Gln Val Pro Trp Met Glu Lys Ala Pro Ala His Leu Ser Leu Pro 
145                 150                 155                 160 


Asp Pro Gln Ala Leu Lys Arg Asp Val Pro Cys Asp Asn Val Ser Ser 
                165                 170                 175     


Cys Pro Ser Ser Asp Thr Cys Cys Gln Leu Thr Ser Gly Glu Trp Gly 
            180                 185                 190         


Cys Cys Pro Ile Pro Glu Ala Val Cys Cys Ser Asp His Gln His Cys 
        195                 200                 205             


Cys Pro Gln Gly Tyr Thr Cys Val Ala Glu Gly Gln Cys Gln 
    210                 215                 220         


<210> 11
<211> 223
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 11
Met Gly Ile Leu Ile Gln Cys Pro Asp Ser Gln Phe Glu Cys Pro Asp 
1               5                   10                  15      


Phe Ser Thr Cys Cys Val Met Val Asp Gly Ser Trp Gly Cys Cys Pro 
            20                  25                  30          


Met Pro Gln Ala Ser Cys Cys Glu Asp Arg Val His Cys Cys Pro His 
        35                  40                  45              


Gly Ala Phe Cys Asp Leu Val His Thr Arg Cys Ile Thr Pro Thr Gly 
    50                  55                  60                  


Thr His Pro Ser Lys Glu Asn Ala Thr Thr Asp Leu Leu Thr Lys Leu 
65                  70                  75                  80  


Pro Ala His Thr Val Gly Asp Val Lys Cys Asp Met Glu Val Ser Cys 
                85                  90                  95      


Pro Asp Gly Tyr Thr Cys Cys Arg Leu Gln Ser Gly Ala Trp Gly Cys 
            100                 105                 110         


Cys Pro Phe Thr Gln Ala Val Cys Cys Glu Asp His Ile His Cys Cys 
        115                 120                 125             


Pro Ala Gly Phe Thr Cys Asp Thr Gln Lys Gly Thr Cys Glu Gln Gly 
    130                 135                 140                 


Pro His Gln Val Pro Trp Met Glu Lys Ala Pro Ala His Leu Ser Leu 
145                 150                 155                 160 


Pro Asp Pro Gln Ala Leu Lys Arg Asp Val Pro Cys Asp Asn Val Ser 
                165                 170                 175     


Ser Cys Pro Ser Ser Asp Thr Cys Cys Gln Leu Thr Ser Gly Glu Trp 
            180                 185                 190         


Gly Cys Cys Pro Ile Pro Glu Ala Val Cys Cys Ser Asp His Gln His 
        195                 200                 205             


Cys Cys Pro Gln Gly Tyr Thr Cys Val Ala Glu Gly Gln Cys Gln 
    210                 215                 220             


<210> 12
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 12
tggcgaggtc tgactgttgt gg                                                22


<210> 13
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 13
gctcatcaaa aagttccctg tgc                                               23


<210> 14
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 14
tacaagctgg ctggtgggga                                                   20


<210> 15
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 15
gtcgcgggtc tcaggacctt                                                   20


<210> 16
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 16
aatctcacag cagcacatca                                                   20


<210> 17
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 17
aaggtgctca tgtcctcatc                                                   20


<210> 18
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 18
ccttcctacc ccaatttcca at                                                22


<210> 19
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 19
gccactcctt ctgtgactcc ag                                                22


<210> 20
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 20
cttcaccacc atggagaagg c                                                 21


<210> 21
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 21
gacggacaca ttgggggtag                                                   20


<210> 22
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 22
Met Gly Ile Leu 
1               


<210> 23
<211> 53
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: Cysteine-rich "granulin" motif
      polypeptide


<220>
<221> MOD_RES
<222> (2)..(7)
<223> Any amino acid and this region may encompass 5-6 
      residues 

<220>
<221> MOD_RES
<222> (9)..(13)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (16)..(23)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (26)..(31)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (34)..(34)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (36)..(37)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (42)..(45)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (47)..(52)
<223> Any amino acid and this region may encompass 5-6 
      residues 

<400> 23
Cys Xaa Xaa Xaa Xaa Xaa Xaa Cys Xaa Xaa Xaa Xaa Xaa Cys Cys Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Cys Cys Xaa Xaa Xaa Xaa Xaa Xaa Cys 
            20                  25                  30          


Cys Xaa Asp Xaa Xaa His Cys Cys Pro Xaa Xaa Xaa Xaa Cys Xaa Xaa 
        35                  40                  45              


Xaa Xaa Xaa Xaa Cys 
    50              


<210> 24
<211> 10
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: C-terminal PGRN motif
      peptide


<220>
<221> MOD_RES
<222> (3)..(3)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (5)..(6)
<223> Any amino acid

<400> 24
Cys Cys Xaa Asp Xaa Xaa His Cys Cys Pro 
1               5                   10  


<210> 25
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 25
His His His His His His 
1               5       


<210> 26
<211> 33
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> misc_feature
<222> (2)..(33)
<223> This region may encompass 2-4 'FLHTRLFV' repeating units

<220> 
<223> See specification as filed for detailed description of
      substitutions and preferred embodiments

<400> 26
Met Phe Leu His Thr Arg Leu Phe Val Phe Leu His Thr Arg Leu Phe 
1               5                   10                  15      


Val Phe Leu His Thr Arg Leu Phe Val Phe Leu His Thr Arg Leu Phe 
            20                  25                  30          


Val 
    


<210> 27
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 27
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210> 28
<211> 154
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 28
Pro Gln Ala Ser Cys Cys Glu Asp Arg Val His Cys Cys Pro His Gly 
1               5                   10                  15      


Ala Phe Cys Asp Leu Val His Thr Arg Cys Ile Thr Pro Thr Gly Thr 
            20                  25                  30          


His Pro Leu Ala Lys Lys Leu Pro Ala Gln Arg Thr Asn Arg Ala Val 
        35                  40                  45              


Ala Leu Ser Ser Ser Ser Lys Glu Asp Ala Thr Thr Asp Leu Leu Thr 
    50                  55                  60                  


Lys Leu Pro Ala His Thr Val Gly Asp Val Lys Cys Asp Met Glu Val 
65                  70                  75                  80  


Ser Cys Pro Asp Gly Tyr Thr Cys Cys Arg Leu Gln Ser Gly Ala Trp 
                85                  90                  95      


Cys Glu Gln Gly Pro His Gln Val Pro Trp Met Glu Lys Ala Pro Ala 
            100                 105                 110         


His Leu Ser Leu Pro Asp Pro Gln Ala Leu Lys Arg Asp Val Pro Cys 
        115                 120                 125             


Asp Asn Val Ser Ser Cys Pro Ser Ser Asp Thr Cys Cys Gln Leu Thr 
    130                 135                 140                 


Ser Gly Glu Trp Gly Cys Cys Pro Ile Pro 
145                 150                 


