                                SEQUENCE LISTING

<110> Jin, Hailing
      The Regents of the University of California 

<120> Citrus Greening (Huanglongbing)-Induced
  Small RNAs Are Potential Early Diagnosis Markers
  

<130> 81906-833465

<140> WO Not yet assigned    
<141> Not yet assigned  

<150> US 61/450,050       
<151> 2011-03-07  

<160> 101

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA399 small RNA sequence with
      ath-miR399b alignment subject

<400> 1
tgccaaagga gagttgccct g                                           21

<210> 2
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA399 small RNA sequence with
      ath-miR399b alignment subject

<400> 2
tgccaaagga gagttgccct a                                           21

<210> 3
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA399 small RNA sequence with
      ath-miR399c alignment subject

<400> 3
tgccaaagga gcgttgccct g                                           21

<210> 4
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA399 small RNA sequence with
      ath-miR399b alignment subject

<400> 4
tgccaaagga gagttgccat g                                           21

<210> 5
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA399 small RNA sequence with
      ath-miR399c alignment subject

<400> 5
tgtcaaagga gagttgccct g                                           21

<210> 6
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA399 small RNA sequence with
      ath-miR399a alignment subject

<400> 6
tgccaaagga gaattgccct g                                           21

<210> 7
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA399 small RNA sequence with
      ath-miR399c alignment subject

<400> 7
tgctaaagga gagttgccct a                                           21

<210> 8
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA399 small RNA sequence with
      ath-miR399f alignment subject

<400> 8
tgccaaagga gatttgcccg g                                           21

<210> 9
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic csi-miR399a small RNA sequence

<400> 9
ugccaaagga gauuugcccg g                                           21

<210> 10
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic csi-miR399b small RNA sequence

<400> 10
ugccaaagga gaguugcccu a                                           21

<210> 11
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic csi-miR399c and csi-miR399e small RNA
      sequence

<400> 11
ugccaaagga gaauugcccu g                                           21

<210> 12
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic csi-miR399d small RNA sequence

<400> 12
ugccaaagga gaguugcccu g                                           21

<210> 13
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic csi-siR1005 small RNA sequence

<400> 13
atagataatg gatcaacggt tata                                        24

<210> 14
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic csi-siR1008 small RNA sequence

<400> 14
tcgaacaagg taaggatgtc a                                           21

<210> 15
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic csi-siR1009 small RNA sequence

<400> 15
cttctaataa acatgcatga a                                           21

<210> 16
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5001 microRNA
      sequence

<400> 16
ugaagcugcc agcaugaucu                                             20

<210> 17
<211> 19
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5001 microRNA star

<400> 17
aucaucuggc aguuucacc                                              19

<210> 18
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5002 microRNA
      sequence

<400> 18
uagauaaaga ugagagaaaa a                                           21

<210> 19
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5002 microRNA star

<400> 19
uuuucucuua ucguuaucug u                                           21

<210> 20
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5003 microRNA
      sequence

<400> 20
uuuguugcau gaugcugaua a                                           21

<210> 21
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5003 microRNA star

<400> 21
cuacccgcau caugcaacaa a                                           21

<210> 22
<211> 24
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5004 microRNA
      sequence

<400> 22
aguguuaggu guagagaagc acga                                        24

<210> 23
<211> 24
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5004 microRNA star

<400> 23
ucucguacuu cucuucacca agca                                        24

<210> 24
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5005 microRNA
      sequence

<400> 24
auucgggacg aguuuacaac                                             20

<210> 25
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5005 microRNA star

<400> 25
cguaaacucg ucucguacuu                                             20

<210> 26
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5006 microRNA
      sequence

<400> 26
aauggcugga uccagcugug g                                           21

<210> 27
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5006 microRNA star

<400> 27
acaauuggau uuagccauua a                                           21

<210> 28
<211> 19
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5007 microRNA
      sequence

<400> 28
augccguauc acgugggag                                              19

<210> 29
<211> 19
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5007 microRNA star

<400> 29
cccacuugau gugucauuc                                              19

<210> 30
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5008 microRNA
      sequence

<400> 30
uuugaugccu ucuuuagucg c                                           21

<210> 31
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5008 microRNA star

<400> 31
gauuaaggag aguuuucagu                                             20

<210> 32
<211> 19
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5009 microRNA
      sequence

<400> 32
gacagaagag agtgagcac                                              19

<210> 33
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5009 microRNA star

<400> 33
gcucgcuccu cuucugucag                                             20

<210> 34
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5010 microRNA
      sequence

<400> 34
guauauaucu ugcaugcaug                                             20

<210> 35
<211> 19
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5010 microRNA star

<400> 35
guauguaaga uacaucccc                                              19

<210> 36
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5011 microRNA
      sequence

<400> 36
uuucucuuau cguuaucugu                                             20

<210> 37
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5011 microRNA star

<400> 37
uagauaaaga ugagagaaaa a                                           21

<210> 38
<211> 22
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5012 microRNA
      sequence

<400> 38
uuguuguuga guguguaugu ua                                          22

<210> 39
<211> 22
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5012 microRNA star

<400> 39
acauaaauac uuaauaauaa uc                                          22

<210> 40
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5013 microRNA
      sequence

<400> 40
ucguccuucu cucauauuuu u                                           21

<210> 41
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic citrus miRNA Csi-miR5013 microRNA star

<400> 41
agagugggug gguggagagg                                             20

<210> 42
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 42
ttccagatag aaggccactc a                                           21

<210> 43
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 43
ttccaccaat cgatcaggat a                                           21

<210> 44
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 44
gcgtatgagg agccatgcat a                                           21

<210> 45
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 45
cttggattta tgaaagacga a                                           21

<210> 46
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 46
ggcagggcta gtgactggag tga                                         23

<210> 47
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 47
acaggccgca aacattttcc t                                           21

<210> 48
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 48
acagaccgca caccttttct t                                           21

<210> 49
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 49
attaggagct aaaattgttg t                                           21

<210> 50
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 50
acgaaatgtg agtagagtgg acag                                        24

<210> 51
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 51
ttccaaaggg atcgcattga                                             20

<210> 52
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 52
tcatttaagg gtttcgtgtt c                                           21

<210> 53
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 53
acgctcggac gaagcacata gatg                                        24

<210> 54
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 54
tatgggattt acctcggcaa a                                           21

<210> 55
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 55
tgtgtggatg aataagattt c                                           21

<210> 56
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 56
tatctggata aaaggctacc c                                           21

<210> 57
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 57
tcatggataa ggtcatgcat t                                           21

<210> 58
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 58
aaaaacttgg aagcgttgga t                                           21

<210> 59
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 59
tcctgccggg ttgcataatc a                                           21

<210> 60
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 60
atagataatg gatcaacggt tata                                        24

<210> 61
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 61
tcatggataa ggtcatgcat c                                           21

<210> 62
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 62
ctgaaagctg aggttgtcct t                                           21

<210> 63
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 63
agtgtcaaaa agagcaatgg cgtc                                        24

<210> 64
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 64
aatccttgga ttaggagtgt ggag                                        24

<210> 65
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 65
atcaataaat caggattggc ggaa                                        24

<210> 66
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 66
cgttagggag tccggagacg t                                           21

<210> 67
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 67
gaataagaca tggagttgga a                                           21

<210> 68
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 68
aggaaatgga cgatacggac gcat                                        24

<210> 69
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 69
tcaagtgagg ttcggtcttt gaa                                         23

<210> 70
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 70
taatcgtggg agacgaagct g                                           21

<210> 71
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 71
cgaaggtccg aggtcgaggt t                                           21

<210> 72
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 72
aggtttgggc ttgttgcaag taga                                        24

<210> 73
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 73
tccgggcgga agacattgtc a                                           21

<210> 74
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 74
aacggaaaga acacaacacg g                                           21

<210> 75
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 75
tgttagcttt ctcggacgca g                                           21

<210> 76
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 76
tcaagtgagg ttctgtcttt ga                                          22

<210> 77
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 77
aaagcaacga ttgtatggcc a                                           21

<210> 78
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 78
tcaagtgagg ttcggtcttg a                                           21

<210> 79
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 79
gaatgtggaa ttaagcgcac caaa                                        24

<210> 80
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 80
tcgaacaagg taaggatgtc a                                           21

<210> 81
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 81
ctggatgcaa ctgtggtacg g                                           21

<210> 82
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 82
ggtgcttccg gatctcagga t                                           21

<210> 83
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic HLB induced citrus siRNA

<400> 83
cacatgggtt agtcgatc                                               18

<210> 84
<211> 1066
<212> DNA
<213> Citrus sinensis

<220> 
<223> citrus homolog of ubiquitin conjugating enzyme E2 (UBC)

<400> 84
cacgcgtccg ggtgaaaaga gcttgggtga agaaggtcag caagaatgga gcattctaga 60
gaaatccctt cctgaaacaa tctatgtccg catcttcgag gatagggtgg atctcatacg 120
ggcagccatt gttggtgcaa agggaactcc ctatcacgac gggctgttct tctttgacat 180
ctttcttcct ccagagtatc ctcatgagcc gcctttagta cactatattt ctggtggact 240
ccgtgtgaac cctaacttgt atgagtccgg aaaggtctgt ctcagcctcc tcaatacttg 300
gacgggttca ggcactgaag tatggaatcc agggggctcc acgattcttc aagttcttct 360
ctccctccaa gctcttgtgc ttaatgagaa accttatttc aacgaggctg gatatgataa 420
gcagatagga agagctgagg gagagaaaaa ctcagtaagc tacaatgaaa atgcattcct 480
tgtgacttgc aagtccatgc tttacctact tcacaagcca cccaagcatt ttaaggagct 540
tgtggaggag cacttcagtc aacgctgcaa atacatttta ttggcttgta aggcatacat 600
ggaaggagct gcagtaggaa ctccctctgg atgcaaagaa aatggagaaa attccaacgg 660
atgttcagtg ggcttcaaaa tcatgcttgc ctaagttatt tccaaagctt gtgcaggcct 720
tttctagtaa gggaatcgat tgcaaccaat ttattgagcc tgaaaactga aaaatgaatg 780
cccttcacgg gttcaaataa ttaaaagaac tcgcatttac cttatgaaca cattcctatc 840
gtgtgaatta aaatgtaaaa taacaaaata tgcttgtgtt tcttctgtgc tctgtttaca 900
tgggcgttga acttgtatta taagattggt tgaataagcg tttgctgctg taatatatgg 960
tgtatcacaa tgtacaataa tgaagaaaca atggtcgcag tggtgtgcct taagatcttt 1020
gtaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa                1066

<210> 85
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic miR399b RT primer

<400> 85
gtcgtatcca gtgcagggtc cgaggtattc gcactggata cgacatgggc            50

<210> 86
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer miR399b RT-F

<400> 86
gcggcggtgc caaaggagag tt                                          22

<210> 87
<211> 16
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer miR399b RT-R

<400> 87
gtgcagggtc cgaggt                                                 16

<210> 88
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic csi-siR1005 RT primer

<400> 88
gtcgtatcca gtgcagggtc cgaggtattc gcactggata cgacgttata            50

<210> 89
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer csi-siR1005 RT-F

<400> 89
gcggcggata gataatggat caacg                                       25

<210> 90
<211> 16
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer csi-siR1005 RT-R

<400> 90
gtgcagggtc cgaggt                                                 16

<210> 91
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic csi-siR1008 RT primer

<400> 91
gtcgtatcca gtgcagggtc cgaggtattc gcactggata cgacatgtca            50

<210> 92
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer csi-siR1008 RT-F

<400> 92
gcggcggtcg aacaaggtaa gg                                          22

<210> 93
<211> 16
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer csi-siR1008 RT-R

<400> 93
gtgcagggtc cgaggt                                                 16

<210> 94
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic csi-siR1009 RT primer

<400> 94
gtcgtatcca gtgcagggtc cgaggtattc gcactggata cgaccatgaa            50

<210> 95
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer csi-siR1009 RT-F

<400> 95
gcggcggctt ctaataaaca tg                                          22

<210> 96
<211> 16
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer csi-siR1009 RT-R

<400> 96
gtgcagggtc cgaggt                                                 16

<210> 97
<211> 28
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic 5' adaptor

<400> 97
guucagaguu cuacaguccg acgaucag                                    28

<210> 98
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> synthetic 3' adaptor

<400> 98
ucguaugccg ucuucugcuu g                                           21

<210> 99
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic miRNA399 consensus sequence

<400> 99
tgyyaaagga gmdttgccmk r                                           21

<210> 100
<211> 33
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic csi-siR1008 with flanking sequences

<400> 100
ccttgttcga acaaggtaag gatgtcattc ttt                              33

<210> 101
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic csi-siR1009 with flanking sequences

<400> 101
cgtcttctaa taaacatgca tgaacttatt                                  30



