                         SEQUENCE LISTING

<110>  Ludwig-Maximilians-Universitaet Muenchen
 
<120>  Novel methods for detecting hydroxymethylcytosine

<130>  LMU14068PCT

<150>  EP 11 001 842.1
<151>  2011-03-04

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  274
<212>  DNA
<213>  Homo sapiens

<400>  1
ctcctgtctc agcctcccta gtagatggga ttacaggcgt gcaccaccac gcccagccca       60

gttaattttt gtatttttag acagacaggg tttcaccatg ttggtcaggc tggtcttgaa      120

ctcctgatct cagatgatgc acctggctcg gcctcgcaaa gtgctgggat tacaggtgtg      180

agccaccgtg cccagccgtt agctcatttt aacacatcct tagtccagcc tgttccaaaa      240

aatctaaagt cagatagctt cctaaacctc aact                                  274


<210>  2
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Nanog-FWD primer

<400>  2
ctcctgtctc agcctcccta                                                   20


<210>  3
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Nanog-REV short primer

<400>  3
agttgaggtt taggaagcta tctg                                              24


<210>  4
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  AT adapter oligonucleotide

<400>  4
gtaaaacgac ggccagtat                                                    19


<210>  5
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  13(-20)-REV primer

<400>  5
actggccgtc gttttac                                                      17


<210>  6
<211>  71
<212>  DNA
<213>  Artificial

<220>
<223>  real time PCR detection product from Nanog promoter

<400>  6
ctgggattac aggtgtgagc caccgtgccc agccgttagc tcattttaac acatactggc       60

cgtcgtttta c                                                            71


<210>  7
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Detection primer

<400>  7
ctgggattac aggtgtgag                                                    19


<210>  8
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  M13(-20) primer

<400>  8
gtaaaacgac ggccagt                                                      17


<210>  9
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  primer for amplifying T4 DNA

<400>  9
gtgaagtaag taataaatgg attg                                              24


<210>  10
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  primer for amplifying T4 DNA

<400>  10
tggagaagga gaatgaagaa taat                                              24


<210>  11
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  primer for amplifying T4 DNA

<400>  11
gccatattga taatgaaatt aaatgta                                           27


<210>  12
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  primer for amplifying T4 DNA

<400>  12
tcagcaattt taatatttcc atcttc                                            26


<210>  13
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer for amplifying T4 DNA

<400>  13
tatactgaag tacttcatca                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer for amplifying T4 DNA

<400>  14
ctttgcgtga tttatatgta                                                   20


<210>  15
<211>  39
<212>  DNA
<213>  Artificial

<220>
<223>  primer for amplifying T4 DNA

<400>  15
ctcgtagact gcgtaccaat ctaactcagg atagttgat                              39


<210>  16
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  primer for amplifying T4 DNA

<400>  16
tatgataagt atgtaggtta tt                                                22


<210>  17
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  primer for amplifying T4 DNA

<400>  17
ctcgtagact gcgtacca                                                     18


<210>  18
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  primer for amplifying T4 DNA

<400>  18
tatgataagt atgtaggtta ttcaa                                             25


<210>  19
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  primer for amplifying nanog DNA

<400>  19
tcaggagttt gggaccagct a                                                 21


<210>  20
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  primer for amplifying nanog DNA

<400>  20
cccccctcaa gcctccta                                                     18


<210>  21
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  linker oligonucleotide


<220>
<221>  misc
<222>  (21)..(21)
<223>  n is g, a, t or c

<220>
<221>  misc_feature
<222>  (21)..(22)
<223>  n is a, c, g, or t

<220>
<221>  misc
<222>  (22)..(22)
<223>  n is g, a, t or c

<400>  21
ctcgtagact gcgtaccatg nn                                                22


<210>  22
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  linker oligonucleotide

<400>  22
catggtacgc agtctaccag                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer for amplifying digestion products from T4 DNA substrates

<400>  23
ctcgtagact gcgtaccatg                                                   20


<210>  24
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  primer for amplifying digestion products from T4 DNA

<400>  24
gagtcagacc ttgctgccaa a                                                 21


<210>  25
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  primer for amplifying digestion products from T4 DNA

<400>  25
gccgtctaag caatggaaga a                                                 21


<210>  26
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  primer for amplifying T4 DNA

<400>  26
tatgataagt atgtaggtta tt                                                22


<210>  27
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Pvu TRS1I recognition sequence


<220>
<221>  misc
<222>  (1)..(1)
<223>  c is hydroxymethylcytosine

<220>
<221>  misc
<222>  (2)..(2)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (3)..(3)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (4)..(4)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (5)..(5)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (6)..(6)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (7)..(7)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (8)..(8)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (9)..(9)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (10)..(10)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (11)..(11)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (12)..(12)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (13)..(13)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (14)..(14)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (15)..(15)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (16)..(16)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (17)..(17)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (18)..(18)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (19)..(19)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (20)..(20)
<223>  n is g, a, t or c

<220>
<221>  misc
<222>  (21)..(21)
<223>  n is g, a, t or c

<400>  27
cnnnnnnnnn nnnnnnnnnn ng                                                22


