                         SEQUENCE LISTING

<110>  UNIVERSITE DE STRASBOURG
       PREVOST, Gilles
       KELLER, Daniel
       MASOUD, Khaldoun
       BARASINO, Charline
 
<120>  DEPLETED ANTI-STAPHYLOCOCCAL ENTEROTOXINS POLYCLONAL ANTIBODIES, 
       PREPARATION AND USES THEREOF

<130>  BIP209387PC00

<150>  EP11156131.2
<151>  2011-02-28

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer BamH1-setBf

<400>  1
agtaaggatc cgagagagtc aaccagatcc taaacca                                37


<210>  2
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EcoR1-setBr

<400>  2
aagtatagaa ttcactaaca cttcatattt actttctaaa at                          42


<210>  3
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer EcoR1-setC1f

<400>  3
aagtatagaa ttcgagagcc aaccagaccc tacgccaga                              39


<210>  4
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EcoR1-setC1r

<400>  4
aagtatagaa ttctactttc actttttata tcaaaatgg                              39


<210>  5
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EcoR1-setDf

<400>  5
aagtatagaa ttcaaacatt cttatgcaga taaaaatcca at                          42


<210>  6
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EcoR1-setDr

<400>  6
aagtatagaa ttcttgaaat ggctttagtg tctgatgtt                              39


<210>  7
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EcoR1-setEf

<400>  7
agtaaggatc cagcgaagaa ataaatgaaa aagattt                                37


<210>  8
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EcoR1-setEr

<400>  8
aagtatagaa ttcagttgtg tataaataca aatcaatat                              39


<210>  9
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EcoR1-seGf

<400>  9
agtaaggatc ccaacccgat cctaaattag acgaac                                 36


<210>  10
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EcoR1-SeGr

<400>  10
aagtatgaat tctcagtgag tattaagaaa tacttccatt tt                          42


<210>  11
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EcoR1-setHf

<400>  11
agtaaggatc cgaagattta cacgataaaa gtgagtt                                37


<210>  12
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EcoR1-setHr

<400>  12
aagtatagaa ttctgatcta gaaattttca ttgattata                              39


<210>  13
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer BamH1-setIf

<400>  13
agtaaggatc ccaaggtgat attggtgtag gtaactt                                37


<210>  14
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EcoR1-setIr

<400>  14
aagtatagaa ttcgttacta tctacatatg atatttcg                               38


<210>  15
<211>  238
<212>  PRT
<213>  Staphylococcus aureus

<400>  15

Gly Pro Leu Gly Ser Ser Glu Lys Ser Glu Glu Ile Asn Glu Lys Asp 
1               5                   10                  15      


Leu Arg Lys Lys Ser Glu Leu Gln Gly Thr Ala Leu Gly Asn Leu Lys 
            20                  25                  30          


Gln Ile Tyr Tyr Tyr Asn Glu Lys Ala Lys Thr Glu Asn Lys Glu Ser 
        35                  40                  45              


His Asp Gln Phe Leu Gln His Thr Ile Leu Phe Lys Gly Phe Phe Thr 
    50                  55                  60                  


Asp His Ser Trp Tyr Asn Asp Leu Leu Val Asp Phe Asp Ser Lys Asp 
65                  70                  75                  80  


Ile Val Asp Lys Tyr Lys Gly Lys Lys Val Asp Leu Tyr Gly Ala Tyr 
                85                  90                  95      


Tyr Gly Tyr Gln Cys Ala Gly Gly Thr Pro Asn Lys Thr Ala Cys Met 
            100                 105                 110         


Tyr Gly Gly Val Thr Leu His Asp Asn Asn Arg Leu Thr Glu Glu Lys 
        115                 120                 125             


Lys Val Pro Ile Asn Leu Trp Leu Asp Gly Lys Gln Asn Thr Val Pro 
    130                 135                 140                 


Leu Glu Thr Val Lys Thr Asn Lys Lys Asn Val Thr Val Gln Glu Leu 
145                 150                 155                 160 


Asp Leu Gln Ala Arg Arg Tyr Leu Gln Glu Lys Tyr Asn Leu Tyr Asn 
                165                 170                 175     


Ser Asp Val Phe Asp Gly Lys Val Gln Arg Gly Leu Ile Val Phe His 
            180                 185                 190         


Thr Ser Thr Glu Pro Ser Val Asn Tyr Asp Leu Phe Gly Ala Gln Gly 
        195                 200                 205             


Gln Tyr Ser Asn Thr Leu Leu Arg Ile Tyr Arg Asp Asn Lys Thr Ile 
    210                 215                 220                 


Asn Ser Glu Asn Met His Ile Asp Ile Tyr Leu Tyr Thr Ser 
225                 230                 235             


<210>  16
<211>  244
<212>  PRT
<213>  Staphylococcus aureus

<400>  16

Gly Pro Leu Gly Ser Glu Ser Gln Pro Asp Pro Lys Pro Asp Glu Leu 
1               5                   10                  15      


His Lys Ser Ser Lys Phe Thr Gly Leu Met Glu Asn Met Lys Val Leu 
            20                  25                  30          


Tyr Asp Asp Asn His Val Ser Ala Ile Asn Val Lys Ser Ile Asp Gln 
        35                  40                  45              


Phe Leu Tyr Phe Asp Leu Ile Tyr Ser Ile Lys Asp Thr Lys Leu Gly 
    50                  55                  60                  


Asn Tyr Asp Asn Val Arg Val Glu Phe Lys Asn Lys Asp Leu Ala Asp 
65                  70                  75                  80  


Lys Tyr Lys Asp Lys Tyr Val Asp Val Phe Gly Ala Asn Tyr Tyr Tyr 
                85                  90                  95      


Gln Cys Tyr Phe Ser Lys Lys Thr Asn Asp Ile Asn Ser His Gln Thr 
            100                 105                 110         


Asp Lys Arg Lys Thr Cys Met Tyr Gly Gly Val Thr Glu His Asn Gly 
        115                 120                 125             


Asn Gln Leu Asp Lys Tyr Arg Ser Ile Thr Val Arg Val Phe Glu Asp 
    130                 135                 140                 


Gly Lys Asn Leu Leu Ser Phe Asp Val Gln Thr Asn Lys Lys Lys Val 
145                 150                 155                 160 


Thr Ala Gln Glu Leu Asp Tyr Leu Thr Arg His Tyr Leu Val Lys Asn 
                165                 170                 175     


Lys Lys Leu Tyr Glu Phe Asn Asn Ser Pro Tyr Glu Thr Gly Tyr Ile 
            180                 185                 190         


Lys Phe Ile Glu Asn Glu Asn Ser Phe Trp Tyr Asp Met Met Pro Ala 
        195                 200                 205             


Pro Gly Asp Lys Phe Asp Gln Ser Lys Tyr Leu Met Met Tyr Asn Asp 
    210                 215                 220                 


Asn Lys Met Val Asp Ser Lys Asp Val Lys Ile Glu Val Tyr Leu Thr 
225                 230                 235                 240 


Thr Lys Lys Lys 
                


<210>  17
<211>  247
<212>  PRT
<213>  Staphylococcus aureus

<400>  17

Gly Pro Leu Gly Ser Pro Glu Phe Glu Ser Gln Pro Asp Pro Thr Pro 
1               5                   10                  15      


Asp Glu Leu His Lys Ala Ser Lys Phe Thr Gly Leu Met Glu Asn Met 
            20                  25                  30          


Lys Val Leu Tyr Asp Asp His Tyr Val Ser Ala Thr Lys Val Lys Ser 
        35                  40                  45              


Val Asp Lys Phe Leu Ala His Asp Leu Ile Tyr Asn Ile Ser Asp Lys 
    50                  55                  60                  


Lys Leu Lys Asn Tyr Asp Lys Val Lys Thr Glu Leu Leu Asn Glu Gly 
65                  70                  75                  80  


Leu Ala Lys Lys Tyr Lys Asp Glu Val Val Asp Val Tyr Gly Ser Asn 
                85                  90                  95      


Tyr Tyr Val Asn Cys Tyr Phe Ser Ser Lys Asp Asn Val Gly Lys Val 
            100                 105                 110         


Thr Gly Gly Lys Thr Cys Met Tyr Gly Gly Ile Thr Lys His Glu Gly 
        115                 120                 125             


Asn His Phe Asp Asn Gly Asn Leu Gln Asn Val Leu Ile Arg Val Tyr 
    130                 135                 140                 


Glu Asn Lys Arg Asn Thr Ile Ser Phe Glu Val Gln Thr Asp Lys Lys 
145                 150                 155                 160 


Ser Val Thr Ala Gln Glu Leu Asp Ile Lys Ala Arg Asn Phe Leu Ile 
                165                 170                 175     


Asn Lys Lys Asn Leu Tyr Glu Phe Asn Ser Ser Pro Tyr Glu Thr Gly 
            180                 185                 190         


Tyr Ile Lys Phe Ile Glu Asn Asn Gly Asn Thr Phe Trp Tyr Asp Met 
        195                 200                 205             


Met Pro Ala Pro Gly Asp Lys Phe Asp Gln Ser Lys Tyr Leu Met Met 
    210                 215                 220                 


Tyr Asn Asp Asn Lys Thr Val Asp Ser Lys Ser Val Lys Ile Glu Val 
225                 230                 235                 240 


His Leu Thr Thr Lys Asn Gly 
                245         


<210>  18
<211>  207
<212>  PRT
<213>  Staphylococcus aureus

<400>  18

Lys His Ser Tyr Ala Asp Lys Asn Pro Ile Ile Gly Glu Asn Lys Ser 
1               5                   10                  15      


Thr Gly Asp Gln Phe Leu Glu Asn Thr Leu Leu Tyr Lys Lys Phe Phe 
            20                  25                  30          


Thr Asp Leu Ile Asn Phe Glu Asp Leu Leu Ile Asn Phe Asn Ser Lys 
        35                  40                  45              


Glu Met Ala Gln His Phe Lys Ser Lys Asn Val Asp Val Tyr Pro Ile 
    50                  55                  60                  


Arg Tyr Ser Ile Asn Cys Tyr Gly Gly Glu Ile Asp Arg Thr Ala Cys 
65                  70                  75                  80  


Thr Tyr Gly Gly Val Thr Pro His Glu Gly Asn Lys Leu Lys Glu Arg 
                85                  90                  95      


Lys Lys Ile Pro Ile Asn Leu Trp Ile Asn Gly Val Gln Lys Glu Val 
            100                 105                 110         


Ser Leu Asp Lys Val Gln Thr Asp Lys Lys Asn Val Thr Val Gln Glu 
        115                 120                 125             


Leu Asp Ala Gln Ala Arg Arg Tyr Leu Gln Lys Asp Leu Lys Leu Tyr 
    130                 135                 140                 


Asn Asn Asp Thr Leu Gly Gly Lys Ile Gln Arg Gly Lys Ile Glu Phe 
145                 150                 155                 160 


Asp Ser Ser Asp Gly Ser Lys Val Ser Tyr Asp Leu Phe Asp Val Lys 
                165                 170                 175     


Gly Asp Phe Pro Glu Lys Gln Leu Arg Ile Tyr Ser Asp Asn Lys Thr 
            180                 185                 190         


Leu Ser Thr Glu His Leu His Ile Asp Ile Tyr Leu Tyr Glu Lys 
        195                 200                 205         


<210>  19
<211>  235
<212>  PRT
<213>  Staphylococcus aureus

<400>  19

Gly Pro Leu Gly Ser Ser Glu Glu Ile Asn Glu Lys Asp Leu Arg Lys 
1               5                   10                  15      


Lys Ser Glu Leu Gln Arg Asn Ala Leu Ser Asn Leu Arg Gln Ile Tyr 
            20                  25                  30          


Tyr Tyr Asn Glu Lys Ala Ile Thr Glu Asn Lys Glu Ser Asp Asp Gln 
        35                  40                  45              


Phe Leu Glu Asn Thr Leu Leu Phe Lys Gly Phe Phe Thr Gly His Pro 
    50                  55                  60                  


Trp Tyr Asn Asp Leu Leu Val Asp Leu Gly Ser Lys Asp Ala Thr Asn 
65                  70                  75                  80  


Lys Tyr Lys Gly Lys Lys Val Asp Leu Tyr Gly Ala Tyr Tyr Gly Tyr 
                85                  90                  95      


Gln Cys Ala Gly Gly Thr Pro Asn Lys Thr Ala Cys Met Tyr Gly Gly 
            100                 105                 110         


Val Thr Leu His Asp Asn Asn Arg Leu Thr Glu Glu Lys Lys Val Pro 
        115                 120                 125             


Ile Asn Leu Trp Ile Asp Gly Lys Gln Thr Thr Val Pro Ile Asp Lys 
    130                 135                 140                 


Val Lys Thr Ser Lys Lys Glu Val Thr Val Gln Glu Leu Asp Leu Gln 
145                 150                 155                 160 


Ala Arg His Tyr Leu His Gly Lys Phe Gly Leu Tyr Asn Ser Asp Ser 
                165                 170                 175     


Phe Gly Gly Lys Val Gln Arg Gly Leu Ile Val Phe His Ser Ser Glu 
            180                 185                 190         


Gly Ser Thr Val Ser Tyr Asp Leu Phe Asp Ala Gln Gly Gln Tyr Pro 
        195                 200                 205             


Asp Thr Leu Leu Arg Ile Tyr Arg Asp Asn Lys Thr Ile Asn Ser Glu 
    210                 215                 220                 


Asn Leu His Ile Asp Leu Tyr Leu Tyr Thr Thr 
225                 230                 235 


<210>  20
<211>  241
<212>  PRT
<213>  Staphylococcus aureus

<400>  20

Gly Pro Leu Gly Ser Pro Glu Phe Gln Pro Asp Pro Lys Leu Asp Glu 
1               5                   10                  15      


Leu Asn Lys Val Ser Asp Tyr Lys Asn Asn Lys Gly Thr Met Gly Asn 
            20                  25                  30          


Val Met Asn Leu Tyr Thr Ser Pro Pro Val Glu Gly Arg Gly Val Ile 
        35                  40                  45              


Asn Ser Arg Gln Phe Leu Ser His Asp Leu Ile Phe Pro Ile Glu Tyr 
    50                  55                  60                  


Lys Ser Tyr Asn Glu Val Lys Thr Glu Leu Glu Asn Thr Glu Leu Ala 
65                  70                  75                  80  


Asn Asn Tyr Lys Asp Lys Lys Val Asp Ile Phe Gly Val Pro Tyr Phe 
                85                  90                  95      


Tyr Thr Cys Ile Ile Pro Lys Ser Glu Pro Asp Ile Asn Gln Asn Phe 
            100                 105                 110         


Gly Gly Cys Cys Met Tyr Gly Gly Leu Thr Phe Asn Ser Ser Glu Asn 
        115                 120                 125             


Glu Arg Asp Lys Leu Ile Thr Val Gln Val Thr Ile Asp Asn Arg Gln 
    130                 135                 140                 


Ser Leu Gly Phe Thr Ile Thr Thr Asn Lys Asn Met Val Thr Ile Gln 
145                 150                 155                 160 


Glu Leu Asp Tyr Lys Ala Arg His Trp Leu Thr Lys Glu Lys Lys Leu 
                165                 170                 175     


Tyr Glu Phe Asp Gly Ser Ala Phe Glu Ser Gly Tyr Ile Lys Phe Thr 
            180                 185                 190         


Glu Lys Asn Asn Thr Ser Phe Trp Phe Asp Leu Phe Pro Lys Lys Glu 
        195                 200                 205             


Leu Val Pro Phe Val Pro Tyr Lys Phe Leu Asn Ile Tyr Gly Asp Asn 
    210                 215                 220                 


Lys Val Val Asp Ser Lys Ser Ile Lys Met Glu Val Phe Leu Asn Thr 
225                 230                 235                 240 


His 
    


<210>  21
<211>  222
<212>  PRT
<213>  Staphylococcus aureus

<400>  21

Gly Pro Leu Gly Ser Glu Asp Leu His Asp Lys Ser Glu Leu Thr Asp 
1               5                   10                  15      


Leu Ala Leu Ala Asn Ala Tyr Gly Gln Tyr Asn His Pro Phe Ile Lys 
            20                  25                  30          


Glu Asn Ile Lys Ser Asp Glu Ile Ser Gly Glu Lys Asp Leu Ile Phe 
        35                  40                  45              


Arg Asn Gln Gly Asp Ser Gly Asn Asp Leu Arg Val Lys Phe Ala Thr 
    50                  55                  60                  


Ala Asp Leu Ala Gln Lys Phe Lys Asn Lys Asn Val Asp Ile Tyr Gly 
65                  70                  75                  80  


Ala Ser Phe Tyr Tyr Lys Cys Glu Lys Ile Ser Glu Asn Ile Ser Glu 
                85                  90                  95      


Cys Leu Tyr Gly Gly Thr Thr Leu Asn Ser Glu Lys Leu Ala Gln Glu 
            100                 105                 110         


Arg Val Ile Gly Ala Asn Val Trp Val Asp Gly Ile Gln Lys Glu Thr 
        115                 120                 125             


Glu Leu Ile Arg Thr Asn Lys Lys Asn Val Thr Leu Gln Glu Leu Asp 
    130                 135                 140                 


Ile Lys Ile Arg Lys Ile Leu Ser Asp Lys Tyr Lys Ile Tyr Tyr Lys 
145                 150                 155                 160 


Asp Ser Glu Ile Ser Lys Gly Leu Ile Glu Phe Asp Met Lys Thr Pro 
                165                 170                 175     


Arg Asp Tyr Ser Phe Asp Ile Tyr Asp Leu Lys Gly Glu Asn Asp Tyr 
            180                 185                 190         


Glu Ile Asp Lys Ile Tyr Glu Asp Asn Lys Thr Leu Lys Ser Asp Asp 
        195                 200                 205             


Ile Ser His Ile Asp Val Asn Leu Tyr Thr Lys Lys Lys Val 
    210                 215                 220         


<210>  22
<211>  223
<212>  PRT
<213>  Staphylococcus aureus

<400>  22

Gly Pro Leu Gly Ser Gln Gly Asp Ile Gly Val Gly Asn Leu Arg Asn 
1               5                   10                  15      


Phe Tyr Thr Lys His Asp Tyr Ile Asp Leu Lys Gly Val Thr Asp Lys 
            20                  25                  30          


Asn Leu Pro Ile Ala Asn Gln Leu Glu Phe Ser Thr Gly Thr Asn Asp 
        35                  40                  45              


Leu Ile Ser Glu Ser Asn Asn Trp Asp Glu Ile Ser Lys Phe Lys Gly 
    50                  55                  60                  


Lys Lys Leu Asp Ile Phe Gly Ile Asp Tyr Asn Gly Pro Cys Lys Ser 
65                  70                  75                  80  


Lys Tyr Met Tyr Gly Gly Ala Thr Leu Ser Gly Gln Tyr Leu Asn Ser 
                85                  90                  95      


Ala Arg Lys Ile Pro Ile Asn Leu Trp Val Asn Gly Lys His Lys Thr 
            100                 105                 110         


Ile Ser Thr Asp Lys Ile Ala Thr Asn Lys Lys Leu Val Thr Ala Gln 
        115                 120                 125             


Glu Ile Asp Val Lys Leu Arg Arg Tyr Leu Gln Glu Glu Tyr Asn Ile 
    130                 135                 140                 


Tyr Gly His Asn Asn Thr Gly Lys Gly Lys Glu Tyr Gly Tyr Lys Ser 
145                 150                 155                 160 


Lys Phe Tyr Ser Gly Phe Asn Asn Gly Lys Val Leu Phe His Leu Asn 
                165                 170                 175     


Asn Glu Lys Ser Phe Ser Tyr Asp Leu Phe Tyr Thr Gly Asp Gly Leu 
            180                 185                 190         


Pro Val Ser Phe Leu Lys Ile Tyr Glu Asp Asn Lys Ile Ile Glu Ser 
        195                 200                 205             


Glu Lys Phe His Leu Asp Val Glu Ile Ser Tyr Val Asp Ser Asn 
    210                 215                 220             


