                               SEQUENCE LISTING

<110> GEO FOSSIL FUELS, LLC
 
<120> ALKALINE MICROBIAL ENHANCED OIL RECOVERY

<130> GFF-0003

<140>
<141>

<150> 61/633,785
<151> 2012-02-17

<150> 61/496,461
<151> 2011-06-13

<150> 61/446,674
<151> 2011-02-25

<160> 21    

<170> PatentIn version 3.5

<210> 1
<211> 440
<212> PRT
<213> Geobacillus thermodenitrificans

<400> 1
Met Thr Lys Lys Ile His Ile Asn Ala Phe Glu Met Asn Cys Val Gly 
1               5                   10                  15      


His Ile Ala His Gly Leu Trp Arg His Pro Glu Asn Gln Arg His Arg 
            20                  25                  30          


Tyr Thr Asp Leu Asn Tyr Trp Thr Glu Leu Ala Gln Leu Leu Glu Lys 
        35                  40                  45              


Gly Lys Phe Asp Ala Leu Phe Leu Ala Asp Val Val Gly Ile Tyr Asp 
    50                  55                  60                  


Val Tyr Arg Gln Ser Arg Asp Thr Ala Val Arg Glu Ala Val Gln Ile 
65                  70                  75                  80  


Pro Val Asn Asp Pro Leu Met Leu Ile Ser Ala Met Ala Tyr Val Thr 
                85                  90                  95      


Lys His Leu Ala Phe Ala Val Thr Phe Ser Thr Thr Tyr Glu His Pro 
            100                 105                 110         


Tyr Gly His Ala Arg Arg Met Ser Thr Leu Asp His Leu Thr Lys Gly 
        115                 120                 125             


Arg Ile Ala Trp Asn Val Val Thr Ser His Leu Pro Ser Ala Asp Lys 
    130                 135                 140                 


Asn Phe Gly Ile Lys Lys Ile Leu Glu His Asp Glu Arg Tyr Asp Leu 
145                 150                 155                 160 


Ala Asp Glu Tyr Leu Glu Val Cys Tyr Lys Leu Trp Glu Gly Ser Trp 
                165                 170                 175     


Glu Asp Asn Ala Val Ile Arg Asp Ile Glu Asn Asn Ile Tyr Thr Asp 
            180                 185                 190         


Pro Ser Lys Val His Glu Ile Asn His Ser Gly Lys Tyr Phe Glu Val 
        195                 200                 205             


Pro Gly Pro His Leu Cys Glu Pro Ser Pro Gln Arg Thr Pro Val Ile 
    210                 215                 220                 


Tyr Gln Ala Gly Met Ser Glu Arg Gly Arg Glu Phe Ala Ala Lys His 
225                 230                 235                 240 


Ala Glu Cys Val Phe Leu Gly Gly Lys Asp Val Glu Thr Leu Lys Phe 
                245                 250                 255     


Phe Val Asp Asp Ile Arg Lys Arg Ala Lys Lys Tyr Gly Arg Asn Pro 
            260                 265                 270         


Asp His Ile Lys Met Phe Ala Gly Ile Cys Val Ile Val Gly Lys Thr 
        275                 280                 285             


His Asp Glu Ala Met Glu Lys Leu Asn Ser Phe Gln Lys Tyr Trp Ser 
    290                 295                 300                 


Leu Glu Gly His Leu Ala His Tyr Gly Gly Gly Thr Gly Tyr Asp Leu 
305                 310                 315                 320 


Ser Lys Tyr Ser Ser Asn Asp Tyr Ile Gly Ser Ile Ser Val Gly Glu 
                325                 330                 335     


Ile Ile Asn Asn Met Ser Lys Leu Asp Gly Lys Trp Phe Lys Leu Ser 
            340                 345                 350         


Val Gly Thr Pro Lys Lys Val Ala Asp Glu Met Gln Tyr Leu Val Glu 
        355                 360                 365             


Glu Ala Gly Ile Asp Gly Phe Asn Leu Val Gln Tyr Val Ser Pro Gly 
    370                 375                 380                 


Thr Phe Val Asp Phe Ile Glu Leu Val Val Pro Glu Leu Gln Lys Arg 
385                 390                 395                 400 


Gly Leu Tyr Arg Val Asp Tyr Glu Glu Gly Thr Tyr Arg Glu Lys Leu 
                405                 410                 415     


Phe Gly Lys Gly Asn Tyr Arg Leu Pro Asp Asp His Ile Ala Ala Arg 
            420                 425                 430         


Tyr Arg Asn Ile Ser Ser Asn Val 
        435                 440 


<210> 2
<211> 461
<212> PRT
<213> Halalkalicoccus jeotgali

<400> 2
Met Thr Asp Glu Arg Met Gly Leu Asn Leu Phe Thr Met Asn Ser Val 
1               5                   10                  15      


Glu His Val Ser Ala Gly Ser Trp Arg Tyr Pro Gly Asp Gln Ser His 
            20                  25                  30          


Arg Tyr Thr Asp Arg Glu Tyr Trp Thr Glu Leu Ala Arg Thr Ala Glu 
        35                  40                  45              


Arg Gly Gly Phe Asp Ala Ile Phe Phe Ala Asp Val Arg Gly Ile Tyr 
    50                  55                  60                  


Asp Val Tyr Asp Asp Gly Arg Glu Thr Ala Ile Glu Arg Ala Val Gln 
65                  70                  75                  80  


Thr Pro Ser Asn His Pro Gln Ala Leu Val Pro Ala Met Ala Glu Val 
                85                  90                  95      


Thr Asp His Leu Gly Phe Ala Ile Thr Arg Ser Thr Thr Tyr Ala His 
            100                 105                 110         


Pro Tyr Gln Leu Ala Arg Glu Phe Ser Thr Leu Asp His Leu Thr Asp 
        115                 120                 125             


Gly Arg Val Ala Phe Asn Val Val Thr Ser Tyr Leu Asp Ser Ala Ala 
    130                 135                 140                 


Arg Asn Leu Gly Leu Glu Gln Arg Met Asp His Asp Gly Arg Tyr Asp 
145                 150                 155                 160 


Arg Ala Ala Glu Phe Met Gln Val Cys Tyr Arg Leu Trp Glu Gly Ser 
                165                 170                 175     


Trp Asp Asp Asp Ala Val Ala Arg Asp Arg Glu Gln Gly Val Tyr Thr 
            180                 185                 190         


Asp Pro Glu Lys Val Arg Glu Ile Asp Phe Glu Gly Glu Tyr Phe Asp 
        195                 200                 205             


Val Gln Gly Pro His Gly Cys Glu Pro Ser Pro Gln Arg Thr Pro Val 
    210                 215                 220                 


Ile Tyr Gln Ala Gly Ser Ser Glu Arg Gly Arg Glu Phe Ala Ala Lys 
225                 230                 235                 240 


Asn Ala Glu Ala Val Phe Thr Ser Gln Pro Thr Glu Lys Gly Val Arg 
                245                 250                 255     


Asp Tyr Met Ala Asp Met Arg Glu Arg Ala Ala Glu Cys Gly Arg Asp 
            260                 265                 270         


Pro Asp Ser Leu Asp Phe Phe Ala Gly Leu Val Pro Ile Val Gly Glu 
        275                 280                 285             


Thr Ala Glu Ile Ala Gln Ala Lys His Glu Ser Tyr Lys Glu Thr Ile 
    290                 295                 300                 


Asp Val Glu Ala Thr Leu Ala Leu Leu Ser Gly Phe Met Asp Met Asp 
305                 310                 315                 320 


Leu Ser Glu Leu Asn Pro Asp Gln Lys Leu Glu His Ile Glu Thr Glu 
                325                 330                 335     


Ala Ile Gln Gly Ala Val Asn Ala Phe Thr Lys Ser Asp Pro Asp Arg 
            340                 345                 350         


Glu Trp Thr Val Arg Glu Met Ala Gln Phe Ala Gly Leu Gly Thr Thr 
        355                 360                 365             


Ser Pro Val Val Val Gly Pro Pro Glu Glu Ile Ala Asp Ala Phe Glu 
    370                 375                 380                 


His Trp Phe His Glu Val Gly Val Asp Gly Phe Asn Val Lys Glu Val 
385                 390                 395                 400 


Ile Arg Pro Asn Ser Leu Arg Asp Phe Val Asp Leu Val Val Pro Glu 
                405                 410                 415     


Leu Arg Glu Arg Gly Leu Val Arg Gly Glu Tyr Glu Asp Gly Thr Leu 
            420                 425                 430         


Arg Glu Ser Leu Thr Gly Arg Ser Glu Leu Ala Glu Asp His Pro Gly 
        435                 440                 445             


Lys Arg Glu Ala Ile Ser Ala Met Gly Leu Arg Glu Leu 
    450                 455                 460     


<210> 3
<211> 458
<212> PRT
<213> Halorubrum lacusprofundi

<400> 3
Met Ser Asp His Leu His Leu Asn Leu Phe Thr Met Ala Ser Val Glu 
1               5                   10                  15      


His Val Ser Pro Gly Ser Trp Thr Tyr Pro Gly Asp Arg Ser Pro Glu 
            20                  25                  30          


Tyr Thr Asp Arg Glu Tyr Trp Thr Glu Val Ala Arg Thr Ala Glu Arg 
        35                  40                  45              


Gly Gly Phe Asp Ala Val Phe Phe Ala Asp Val Arg Gly Ile Tyr Asp 
    50                  55                  60                  


Val Tyr Gly Asp Asp Arg Glu Thr Ala Val Glu Lys Ala Val Gln Thr 
65                  70                  75                  80  


Pro Ala Ser Asp Pro Gln Leu Val Val Pro Ala Met Ala Glu Val Thr 
                85                  90                  95      


Asp Asp Leu Gly Phe Ala Ile Thr Arg Ser Thr Thr Tyr Thr His Pro 
            100                 105                 110         


Tyr Gln Leu Ala Arg Glu Phe Ser Thr Leu Asp His Leu Thr Asp Gly 
        115                 120                 125             


Arg Val Ala Ile Asn Val Val Thr Ser Tyr Leu Gln Ser Ala Ala Glu 
    130                 135                 140                 


Asn Leu Gly Leu Ser Glu Arg Met Asp Lys Gln Thr Arg Tyr Asp Arg 
145                 150                 155                 160 


Ala Asp Glu Phe Leu Asp Val Cys Tyr Lys Leu Trp Glu Glu Ser Trp 
                165                 170                 175     


Asp Asp Asp Ala Val Glu Ile Asp Arg Glu Ala Gly Arg Tyr Thr Asp 
            180                 185                 190         


Pro Glu Lys Val Ser Thr Ile Asp His Glu Gly Glu His Phe Ser Val 
        195                 200                 205             


Pro Gly Pro His Gly Cys Glu Pro Ser Pro Gln Arg Thr Pro Val Ile 
    210                 215                 220                 


Tyr Gln Ala Gly Ser Ser Asp Arg Gly Arg Glu Phe Ala Ala Ala Asn 
225                 230                 235                 240 


Ala Glu Ala Val Phe Ala Ser Gln Pro Thr Glu Glu Gly Val Arg Glu 
                245                 250                 255     


Tyr Val Thr Asp Val Lys Ser Arg Ala Ala Asp His Gly Arg Asp Pro 
            260                 265                 270         


Glu Ser Leu Arg Phe Phe Ile Gly Val Val Pro Val Val Gly Glu Thr 
        275                 280                 285             


Glu Ala Ala Ala Glu Ala Lys His Glu Ala Tyr Lys Ser His Val Asp 
    290                 295                 300                 


Val Glu Ala Thr Leu Ala Leu Leu Ser Gly Phe Leu Asp Met Asp Leu 
305                 310                 315                 320 


Ser Glu Leu Asp Pro Asp Gln Lys Val Glu His Ile Glu Thr Asp Ala 
                325                 330                 335     


Ile Gln Gly Thr Met Asn Ala Phe Thr Lys Ala Gln Pro Asp Arg Glu 
            340                 345                 350         


Trp Thr Val Arg Glu Val Ala Glu Phe Cys Gly Leu Gly Thr Thr Ser 
        355                 360                 365             


Pro Thr Ile Val Gly Thr Pro Glu Gln Val Val Asp Asp Leu Glu His 
    370                 375                 380                 


Trp His Glu Ala Val Gly Val Asp Gly Phe Asn Val Lys Glu Val Val 
385                 390                 395                 400 


Arg Pro Asp Ser Leu Thr Asp Phe Val Asp Leu Val Val Pro Glu Leu 
                405                 410                 415     


Arg Glu Arg Gly Leu Val Pro Asp Pro Asp Asp Ala Gly Asp Ser Pro 
            420                 425                 430         


Arg Gly Asp Gly Thr Leu Arg Glu Arg Leu Leu Gly Thr Gly Gln Ser 
        435                 440                 445             


Gln Leu Arg Asp Asp His Pro Ala Lys Gln 
    450                 455             


<210> 4
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 4
cctacgggag gcagcag                                                      17


<210> 5
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 5
ccccgtcaat tcmtttgagt tt                                                22


<210> 6
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 6
ttccggttga tccygccgga                                                   20


<210> 7
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 7
yccggcgttg amtccaatt                                                    19


<210> 8
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (3)..(3)
<223> Leu or Met

<220>
<221> MOD_RES
<222> (4)..(4)
<223> Any amino acid

<400> 8
His Glu Xaa Xaa His Lys 
1               5       


<210> 9
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (3)..(4)
<223> Any amino acid

<400> 9
Glu His Xaa Xaa Gly His His 
1               5           


<210> 10
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (5)..(5)
<223> Ser or Ala

<400> 10
Leu Gln Arg His Xaa Asp His His Ala 
1               5                   


<210> 11
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 11
ccttggaggt accaggaggt ttttattatg cggcgcgaaa gtctgttgg                   49


<210> 12
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 12
aaccaaggtc tagatcatta tcaggacgca gccttcagcc atcg                        44


<210> 13
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 13
ccttggagtc gacctcgcgt tacgctcttt ctgt                                   34


<210> 14
<211> 65
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 14
ggcccttgca aaccgatacc aacagacttt cgcgccgcat taaaatttcc tccttgaaat       60

gtgtt                                                                   65


<210> 15
<211> 26
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 15
atgcggcgcg aaagtctgtt ggtatc                                            26


<210> 16
<211> 100
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 16
cgagagctca agtaacggtt gagcgaaaaa taaaaaagac ctggaataaa tccagatctt       60

taaatggaag caaattatca ggacgcagcc ttcagccatc                            100


<210> 17
<211> 82
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 17
aaagacaacc tgcaggcgga aaacacattt caaggaggaa attttaatgg aaataacttt       60

ttacccttta acggatgcac aa                                                82


<210> 18
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 18
aacgtttacc tgcaggcttg tggttggtgt aaacttaatc c                           41


<210> 19
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 19
ccttggaaag cttctcgcgt tacgctcttt ctgt                                   34


<210> 20
<211> 52
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 20
aaagacaacc tgcaggagtt acttggagga tactgtgagg agagacaatc ag               52


<210> 21
<211> 53
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 21
cgatccatat ttaaataaaa ttggggccct cctgaatatg gtgctcattg gtg              53


