                         SEQUENCE LISTING

<110>  Universit de Lorraine
 
<120>  DOSAGE D'ARSENIC PAR ELISA

<130>  BNT208074PC00

<150>  FR1151566
<151>  2011-02-25

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  254
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Opars1 : rgion opratrice de l'opron ars d'Escherichia coli

<400>  1
accaactcag ggctggaaag taaaaaaccg acgcaaagtc ggttttttta cgtcctgatt       60

cagacctcct ttcaaatgaa tagccaactc aaaattcaca cctattacct tcctctgcac      120

ttacacattc gttaagtcat atatgttttt gacttatccg cttcgaagag agacactacc      180

tgcaacaatc aggagcgcaa tatgtcattt ctgttaccca tccaattgtt caaaattctt      240

gctgatgaaa cccg                                                        254


<210>  2
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce Opars1L

<400>  2
accaactcag ggctggaaa                                                    19


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce Opars1R

<400>  3
cgggtttcat cagcaagaat                                                   20


<210>  4
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Squence de la protine ArsR d'Escherichia coli K12 souche MG1655

<400>  4

Met Ser Phe Leu Leu Pro Ile Gln Leu Phe Lys Ile Leu Ala Asp Glu 
1               5                   10                  15      


Thr Arg Leu Gly Ile Val Leu Leu Leu Ser Glu Leu Gly Glu Leu Cys 
            20                  25                  30          


Val Cys Asp Leu Cys Thr Ala Leu Asp Gln Ser Gln Pro Lys Ile Ser 
        35                  40                  45              


Arg His Leu Ala Leu Leu Arg Glu Ser Gly Leu Leu Leu Asp Arg Lys 
    50                  55                  60                  


Gln Gly Lys Trp Val His Tyr Arg Leu Ser Pro His Ile Pro Ala Trp 
65                  70                  75                  80  


Ala Ala Lys Ile Ile Asp Glu Ala Trp Arg Cys Glu Gln Glu Lys Val 
                85                  90                  95      


Gln Ala Ile Val Arg Asn Leu Ala Arg Gln Asn Cys Ser Gly Asp Ser 
            100                 105                 110         


Lys Asn Ile Cys Ser 
        115         


<210>  5
<211>  384
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Squence du gne arsR d'Escherichia coli K12 souche MG1655

<400>  5
atgcttgatt ataaagatga tgatgataaa ggctcatttc tgttacccat ccaattgttc       60

aaaattcttg ctgatgaaac ccgtctgggc atcgttttac tgctcagcga actgggagag      120

ttatgcgtct gcgatctctg cactgctctc gaccagtcgc agcccaagat ctcccgccac      180

ctggcattgc tgcgtgaaag cgggctattg ctggaccgca agcaaggtaa gtgggttcat      240

taccgcttat caccgcatat tccagcatgg gcggcgaaaa ttattgatga ggcctggcga      300

tgtgaacagg aaaaggttca ggcgattgtc cgcaacctgg ctcgacaaaa ctgttccggg      360

gacagtaaga acatttgcag ttaa                                             384


<210>  6
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce arsRR

<400>  6
cttggatcct tagcctttat catcatcatc tttataatca ctgcaaatgt tcttactgtc       60

c                                                                       61


<210>  7
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amorce arsRL

<400>  7
ctagcatgct tgattataaa gatgatgatg ataaaggctc atttctgtta cccatccaa        59


<210>  8
<211>  381
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  squence du gne arsR d'Escherichia coli K12 souche MG1655 dans 
       lequel la squence codante pour le peptide Flag a t insre en 
       position N-terminale, en aval du codon initiation ATG

<400>  8
atggattata aagatgatga tgataaaggc tcatttctgt tacccatcca attgttcaaa       60

attcttgctg atgaaacccg tctgggcatc gttttactgc tcagcgaact gggagagtta      120

tgcgtctgcg atctctgcac tgctctcgac cagtcgcagc ccaagatctc ccgccacctg      180

gcattgctgc gtgaaagcgg gctattgctg gaccgcaagc aaggtaagtg ggttcattac      240

cgcttatcac cgcatattcc agcatgggcg gcgaaaatta ttgatgaggc ctggcgatgt      300

gaacaggaaa aggttcaggc gattgtccgc aacctggctc gacaaaactg ttccggggac      360

agtaagaaca tttgcagtta a                                                381


<210>  9
<211>  127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Squence de la protine ArsRFlagN

<400>  9

Met Leu Asp Tyr Lys Asp Asp Asp Asp Lys Gly Ser Phe Leu Leu Pro 
1               5                   10                  15      


Ile Gln Leu Phe Lys Ile Leu Ala Asp Glu Thr Arg Leu Gly Ile Val 
            20                  25                  30          


Leu Leu Leu Ser Glu Leu Gly Glu Leu Cys Val Cys Asp Leu Cys Thr 
        35                  40                  45              


Ala Leu Asp Gln Ser Gln Pro Lys Ile Ser Arg His Leu Ala Leu Leu 
    50                  55                  60                  


Arg Glu Ser Gly Leu Leu Leu Asp Arg Lys Gln Gly Lys Trp Val His 
65                  70                  75                  80  


Tyr Arg Leu Ser Pro His Ile Pro Ala Trp Ala Ala Lys Ile Ile Asp 
                85                  90                  95      


Glu Ala Trp Arg Cys Glu Gln Glu Lys Val Gln Ala Ile Val Arg Asn 
            100                 105                 110         


Leu Ala Arg Gln Asn Cys Ser Gly Asp Ser Lys Asn Ile Cys Ser 
        115                 120                 125         


<210>  10
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide de reconnaissance : Flag

<400>  10

Asp Tyr Lys Asp Asp Asp Asp Lys Gly 
1               5                   


<210>  11
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide de reconnaissance : peptide V5

<400>  11

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  12
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide de reconnaissance  : His

<400>  12

His His His His His His 
1               5       


<210>  13
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide de reconnaissance : c-MYC

<400>  13

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  14
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide de reconnaissance : HA

<400>  14

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  15
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide de reconnaissance : VSV-G

<400>  15

Tyr Thr Asp Ile Glu Met Asn Arg Leu Gly Lys 
1               5                   10      


<210>  16
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide de reconnaissance : AU5

<400>  16

Thr Asp Phe Tyr Leu Lys 
1               5       


<210>  17
<211>  127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli R-factor R773 arsR gene (X16045)

<400>  17
gaattccaag ttatctcacc taccttaagg taatagtgtg attaatcata tgcgtttttg       60

gttatgtgtt gtttgactta atatcagagc cgagagatac ttgttttcta caaaggagag      120

ggaaatg                                                                127


<210>  18
<211>  380
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ADNc codant pour ArsRFlagN optimise

<400>  18
ccatggacta caaagacgac gacgacaaag gtagctttct gctgccgatc cagctgttca       60

aaattctggc agacgaaacc cgtctgggta ttgtgttact gctgagcgaa ttaggcgaac      120

tgtgcgtttg cgatctgtgt accgcgttag atcagagtca gccgaaaatt agccgtcatc      180

tggcgttatt acgcgaaagc ggtctgttac tggaccgtaa acagggcaaa tgggtccatt      240

accgcttatc tccgcatatt ccggcttggg cagcaaaaat cattgacgaa gcctggcgtt      300

gcgaacagga aaaagttcag gcgatcgtcc gtaacctggc acgtcaaaat tgtagcggcg      360

atagcaaaaa catctcgagc                                                  380


<210>  19
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Squence Tag

<400>  19
gactacaaag acgacgacga caaa                                              24


<210>  20
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  squence optimise du gne arsR

<400>  20
ggtagctttc tgctgccgat ccagctgttc aaaattctgg cagacgaaac ccgtctgggt       60

attgtgttac tgctgagcga attaggcgaa ctgtgcgttt gcgatctgtg taccgcgtta      120

gatcagagtc agccgaaaat tagccgtcat ctggcgttat tacgcgaaag cggtctgtta      180

ctggaccgta aacagggcaa atgggtccat taccgcttat ctccgcatat tccggcttgg      240

gcagcaaaaa tcattgacga agcctggcgt tgcgaacagg aaaaagttca ggcgatcgtc      300

cgtaacctgg cacgtcaaaa ttgtagcggc gatagcaaaa acatc                      345


<210>  21
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ADNc de ArsRFlagN-6His

<400>  21
atggactaca aagacgacga cgacaaaggt agctttctgc tgccgatcca gctgttcaaa       60

attctggcag acgaaacccg tctgggtatt gtgttactgc tgagcgaatt aggcgaactg      120

tgcgtttgcg atctgtgtac cgcgttagat cagagtcagc cgaaaattag ccgtcatctg      180

gcgttattac gcgaaagcgg tctgttactg gaccgtaaac agggcaaatg ggtccattac      240

cgcttatctc cgcatattcc ggcttgggca gcaaaaatca ttgacgaagc ctggcgttgc      300

gaacaggaaa aagttcaggc gatcgtccgt aacctggcac gtcaaaattg tagcggcgat      360

agcaaaaaca tctcgagcca ccaccaccac caccactga                             399


<210>  22
<211>  132
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protine ArsRFlagN-6His

<400>  22

Met Asp Tyr Lys Asp Asp Asp Asp Lys Gly Ser Phe Leu Leu Pro Ile 
1               5                   10                  15      


Gln Leu Phe Lys Ile Leu Ala Asp Glu Thr Arg Leu Gly Ile Val Leu 
            20                  25                  30          


Leu Leu Ser Glu Leu Gly Glu Leu Cys Val Cys Asp Leu Cys Thr Ala 
        35                  40                  45              


Leu Asp Gln Ser Gln Pro Lys Ile Ser Arg His Leu Ala Leu Leu Arg 
    50                  55                  60                  


Glu Ser Gly Leu Leu Leu Asp Arg Lys Gln Gly Lys Trp Val His Tyr 
65                  70                  75                  80  


Arg Leu Ser Pro His Ile Pro Ala Trp Ala Ala Lys Ile Ile Asp Glu 
                85                  90                  95      


Ala Trp Arg Cys Glu Gln Glu Lys Val Gln Ala Ile Val Arg Asn Leu 
            100                 105                 110         


Ala Arg Gln Asn Cys Ser Gly Asp Ser Lys Asn Ile Ser Ser His His 
        115                 120                 125             


His His His His 
    130         


<210>  23
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Squence fixant ArsR Pars1L biotinyl

<400>  23
ctgcacttac acattcgtta agtcatatat gtttttgact t                           41


<210>  24
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Squence fixant ArsR Pars1R

<400>  24
aagtcaaaaa catatatgac ttaacgaatg tgtaagtgc                              39


<210>  25
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Squence fixant ArsR Pars2L biotinyl

<400>  25
ttttttttgc gcattcgtta agtcatatat gtttttgact t                           41


<210>  26
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Squence fixant ArsR Pars2R

<400>  26
aagtcaaaaa catatatgac ttaacgaatc ccc                                    33


