                         SEQUENCE LISTING

<110>  Universidad de Santiago de Compostela
 
<120>  pTOP: Nuevo vector de expresin y purificacin de protenas

<130>  PCT1596.30

<150>  ES201130235
<151>  2011-02-23

<160>  23    

<170>  PatentIn version 3.5

<210>  1
<211>  4854
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pTOP

<400>  1
aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct       60

tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca      120

aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg      180

attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg      240

atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg      300

ctaacaggag gaattaacca tgggccacca ccatcaccac caccatcatc atcatggcgg      360

cagatcttcc cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact      420

tcttttggaa tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga      480

taaatggcga aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat      540

tgatggtgat gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca      600

caacatgttg ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt      660

tttggatatt agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa      720

agttgatttt cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca      780

taaaacatat ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct      840

tgatgttgtt ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt      900

taaaaaacgt attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat      960

agcatggcct ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc     1020

ggatctggaa gttctgttcc aggggcccca tcaccatcac catcaccatc accatcacga     1080

tgacgatgac aagtaaggaa gacggagttg tcattggaat tcgaagcttg ggcccgaaca     1140

aaaactcatc tcagaagagg atctgaatag cgccgtcgac catcatcatc atcatcattg     1200

agtttaaacg gtctccagct tggctgtttt ggcggatgag agaagatttt cagcctgata     1260

cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc     1320

gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt     1380

agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc     1440

tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag     1500

taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg     1560

ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga     1620

tggccttttt gcgtttctac aaactctttt tgtttatttt tctaaataca ttcaaatatg     1680

tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt     1740

atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct     1800

gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca     1860

cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc     1920

gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc     1980

cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg     2040

gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta     2100

tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc     2160

ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt     2220

gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg     2280

cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct     2340

tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc     2400

tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct     2460

cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac     2520

acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc     2580

tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat     2640

ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg     2700

accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc     2760

aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa     2820

ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag     2880

gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta     2940

ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta     3000

ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag     3060

ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg     3120

gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg     3180

cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag     3240

cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc     3300

cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa     3360

aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg     3420

ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct     3480

gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa     3540

gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg     3600

tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat     3660

cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct     3720

gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct     3780

gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcag atcaattcgc     3840

gcgcgaaggc gaagcggcat gcataatgtg cctgtcaaat ggacgaagca gggattctgc     3900

aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat tatgacaact     3960

tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc     4020

cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac     4080

cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt     4140

ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac     4200

gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca     4260

ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg     4320

actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca     4380

gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga     4440

aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc     4500

agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt     4560

cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat     4620

cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg     4680

tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac     4740

cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca     4800

gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc agag           4854


<210>  2
<211>  4731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Secuencia del plsmido pPreTOP1-Tfam

<400>  2
aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct       60

tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca      120

aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg      180

attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg      240

atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg      300

ctaacaggag gaattaacca tgggccacca ccatcaccac caccatcatc atcatggcgg      360

atccttttcc agcatgggta gctatccaaa gaaacctatg agttcatacc ttcgattttc      420

cacagaacag ctacccaaat ttaaagctaa acacccagat gcaaaacttt cagaattggt      480

taggaaaatt gcagccctgt ggagggagct accagaagca gaaaaaaagg tttatgaagc      540

tgattttaaa gctgagtgga aagcatacaa agaagctgtg agcaagtata aagagcagct      600

aactccaagt cagctgatgg gtatggagaa ggaggcccgg cagagacggt taaaaaagaa      660

agcactggta aagagaagag aattaatttt gcttggaaaa ccaaaaagac ctcgttcagc      720

atataacatt tatgtatctg aaagcttcca ggaggcaaag gatgattcgg ctcagggaaa      780

attgaagctt gtaaatgagg cttggaaaaa tctgtctcct gaggaaaagc aggcatatat      840

tcagcttgct aaagatgata ggattcgtta cgacaatgaa atgaagtctt gggaagagca      900

gatggctgaa gttggacgaa gtgatctcat ccgtcgaagt gtgaaacgat ccggagacat      960

ctctgagcat taaggaagac ggagttgtca ttggaattcg aagcttgggc ccgaacaaaa     1020

actcatctca gaagaggatc tgaatagcgc cgtcgaccat catcatcatc atcattgagt     1080

ttaaacggtc tccagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag     1140

attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg     1200

gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt     1260

gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca     1320

gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag     1380

gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc     1440

aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg     1500

cctttttgcg tttctacaaa ctctttttgt ttatttttct aaatacattc aaatatgtat     1560

ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg     1620

agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt     1680

tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga     1740

gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa     1800

gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt     1860

gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt     1920

gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc     1980

agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga     2040

ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat     2100

cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct     2160

gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc     2220

cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg     2280

gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc     2340

ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg     2400

acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca     2460

ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta     2520

aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc     2580

aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa     2640

ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca     2700

ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta     2760

actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc     2820

caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca     2880

gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta     2940

ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag     3000

cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt     3060

cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc     3120

acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac     3180

ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac     3240

gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc     3300

tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat     3360

accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag     3420

cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc     3480

actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc     3540

tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac     3600

gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca     3660

tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg     3720

cgaaggcgaa gcggcatgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa     3780

ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga     3840

cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg     3900

tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga     3960

cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt     4020

cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg     4080

acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt     4140

gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact     4200

cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct     4260

ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat     4320

gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt     4380

taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc     4440

gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac     4500

ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga     4560

gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt     4620

tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca     4680

ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga g              4731


<210>  3
<211>  4734
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Secuencia de pPreTOP2-TFAM

<400>  3
aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct       60

tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca      120

aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg      180

attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg      240

atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg      300

ctaacaggag gaattaacca tgggccacca ccatcaccac caccatcatc atcatggcgg      360

cagatctttt tccagcatgg gtagctatcc aaagaaacct atgagttcat accttcgatt      420

ttccacagaa cagctaccca aatttaaagc taaacaccca gatgcaaaac tttcagaatt      480

ggttaggaaa attgcagccc tgtggaggga gctaccagaa gcagaaaaaa aggtttatga      540

agctgatttt aaagctgagt ggaaagcata caaagaagct gtgagcaagt ataaagagca      600

gctaactcca agtcagctga tgggtatgga gaaggaggcc cggcagagac ggttaaaaaa      660

gaaagcactg gtaaagagaa gagaattaat tttgcttgga aaaccaaaaa gacctcgttc      720

agcatataac atttatgtat ctgaaagctt ccaggaggca aaggatgatt cggctcaggg      780

aaaattgaag cttgtaaatg aggcttggaa aaatctgtct cctgaggaaa agcaggcata      840

tattcagctt gctaaagatg ataggattcg ttacgacaat gaaatgaagt cttgggaaga      900

gcagatggct gaagttggac gaagtgatct catccgtcga agtgtgaaac gatccggaga      960

catctctgag cattaaggaa gacggagttg tcattggaat tcgaagcttg ggcccgaaca     1020

aaaactcatc tcagaagagg atctgaatag cgccgtcgac catcatcatc atcatcattg     1080

agtttaaacg gtctccagct tggctgtttt ggcggatgag agaagatttt cagcctgata     1140

cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc     1200

gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt     1260

agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc     1320

tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag     1380

taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg     1440

ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga     1500

tggccttttt gcgtttctac aaactctttt tgtttatttt tctaaataca ttcaaatatg     1560

tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt     1620

atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct     1680

gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca     1740

cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc     1800

gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc     1860

cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg     1920

gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta     1980

tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc     2040

ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt     2100

gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg     2160

cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct     2220

tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc     2280

tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct     2340

cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac     2400

acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc     2460

tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat     2520

ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg     2580

accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc     2640

aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa     2700

ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag     2760

gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta     2820

ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta     2880

ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag     2940

ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg     3000

gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg     3060

cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag     3120

cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc     3180

cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa     3240

aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg     3300

ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct     3360

gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa     3420

gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg     3480

tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat     3540

cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct     3600

gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct     3660

gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcag atcaattcgc     3720

gcgcgaaggc gaagcggcat gcataatgtg cctgtcaaat ggacgaagca gggattctgc     3780

aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat tatgacaact     3840

tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc     3900

cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac     3960

cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt     4020

ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac     4080

gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca     4140

ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg     4200

actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca     4260

gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga     4320

aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc     4380

agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt     4440

cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat     4500

cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg     4560

tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac     4620

cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca     4680

gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc agag           4734


<210>  4
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador directo para la clonacin de la diana de de pre-Scission 
       en pPRETOP2-Tfam que contiene las dianas de Bgl II y NheI

<400>  4
ttttagatct ctggaagttc tgttccaggg gcccgctagc ttttccagca tgggtagcta       60


<210>  5
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador inverso para la clonacin de la diana de pre-Scission en 
       pPRETOP2-Tfam

<400>  5
catacccatc agctgacttg gagttag                                           27


<210>  6
<211>  4764
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Secuencia del plsmido pPreTOP3-Tfam

<400>  6
aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct       60

tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca      120

aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg      180

attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg      240

atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg      300

ctaacaggag gaattaacca tgggccacca ccatcaccac caccatcatc atcatggcgg      360

cagatctctg gaagttctgt tccaggggcc cgctagcttt tccagcatgg gtagctatcc      420

aaagaaacct atgagttcat accttcgatt ttccacagaa cagctaccca aatttaaagc      480

taaacaccca gatgcaaaac tttcagaatt ggttaggaaa attgcagccc tgtggaggga      540

gctaccagaa gcagaaaaaa aggtttatga agctgatttt aaagctgagt ggaaagcata      600

caaagaagct gtgagcaagt ataaagagca gctaactcca agtcagctga tgggtatgga      660

gaaggaggcc cggcagagac ggttaaaaaa gaaagcactg gtaaagagaa gagaattaat      720

tttgcttgga aaaccaaaaa gacctcgttc agcatataac atttatgtat ctgaaagctt      780

ccaggaggca aaggatgatt cggctcaggg aaaattgaag cttgtaaatg aggcttggaa      840

aaatctgtct cctgaggaaa agcaggcata tattcagctt gctaaagatg ataggattcg      900

ttacgacaat gaaatgaagt cttgggaaga gcagatggct gaagttggac gaagtgatct      960

catccgtcga agtgtgaaac gatccggaga catctctgag cattaaggaa gacggagttg     1020

tcattggaat tcgaagcttg ggcccgaaca aaaactcatc tcagaagagg atctgaatag     1080

cgccgtcgac catcatcatc atcatcattg agtttaaacg gtctccagct tggctgtttt     1140

ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg     1200

ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac     1260

tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg     1320

aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat     1380

ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa     1440

cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca     1500

tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt     1560

tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa     1620

atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt     1680

attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa     1740

gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac     1800

agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt     1860

aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt     1920

cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat     1980

cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac     2040

actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg     2100

cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc     2160

ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa     2220

ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag     2280

gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct     2340

gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat     2400

ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa     2460

cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac     2520

caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc     2580

taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc     2640

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg     2700

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg     2760

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca     2820

aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg     2880

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg     2940

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga     3000

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac     3060

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat     3120

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc     3180

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga     3240

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc     3300

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg     3360

gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag     3420

cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg     3480

catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc     3540

gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc     3600

gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt     3660

acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac     3720

cgaaacgcgc gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcataatgtg     3780

cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa     3840

ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac     3900

aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat     3960

agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc     4020

tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc     4080

ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga     4140

cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc     4200

gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta     4260

acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg     4320

cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa     4380

agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg     4440

gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat     4500

gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct     4560

gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca     4620

gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca     4680

ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac     4740

ttttcatact cccgccattc agag                                            4764


<210>  7
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador directo para la clonacin de la secuencia que codifica 
       para la GST en pPRETOP3-Tfam

<400>  7
catcatcatg gcggcagatc ttcccctata ctagg                                  35


<210>  8
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador inverso para la clonacin de la secuencia que codifica 
       para la GST en pPRETOP3-Tfam

<400>  8
aaagctagcg ggcccctgga acagaacttc                                        30


<210>  9
<211>  5421
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Secuencia del plsmido pPreTOP4-Tfam

<400>  9
aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct       60

tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca      120

aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg      180

attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg      240

atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg      300

ctaacaggag gaattaacca tgggccacca ccatcaccac caccatcatc atcatggcgg      360

cagatcttcc cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact      420

tcttttggaa tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga      480

taaatggcga aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat      540

tgatggtgat gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca      600

caacatgttg ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt      660

tttggatatt agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa      720

agttgatttt cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca      780

taaaacatat ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct      840

tgatgttgtt ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt      900

taaaaaacgt attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat      960

agcatggcct ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc     1020

ggatctggaa gttctgttcc aggggcccgc tagcttttcc agcatgggta gctatccaaa     1080

gaaacctatg agttcatacc ttcgattttc cacagaacag ctacccaaat ttaaagctaa     1140

acacccagat gcaaaacttt cagaattggt taggaaaatt gcagccctgt ggagggagct     1200

accagaagca gaaaaaaagg tttatgaagc tgattttaaa gctgagtgga aagcatacaa     1260

agaagctgtg agcaagtata aagagcagct aactccaagt cagctgatgg gtatggagaa     1320

ggaggcccgg cagagacggt taaaaaagaa agcactggta aagagaagag aattaatttt     1380

gcttggaaaa ccaaaaagac ctcgttcagc atataacatt tatgtatctg aaagcttcca     1440

ggaggcaaag gatgattcgg ctcagggaaa attgaagctt gtaaatgagg cttggaaaaa     1500

tctgtctcct gaggaaaagc aggcatatat tcagcttgct aaagatgata ggattcgtta     1560

cgacaatgaa atgaagtctt gggaagagca gatggctgaa gttggacgaa gtgatctcat     1620

ccgtcgaagt gtgaaacgat ccggagacat ctctgagcat taaggaagac ggagttgtca     1680

ttggaattcg aagcttgggc ccgaacaaaa actcatctca gaagaggatc tgaatagcgc     1740

cgtcgaccat catcatcatc atcattgagt ttaaacggtc tccagcttgg ctgttttggc     1800

ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata     1860

aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca     1920

gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac     1980

tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg     2040

ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt     2100

tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca     2160

aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctctttttgt     2220

ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg     2280

cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt     2340

cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta     2400

aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc     2460

ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa     2520

gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc     2580

cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt     2640

acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact     2700

gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac     2760

aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata     2820

ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta     2880

ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg     2940

gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat     3000

aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt     3060

aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga     3120

aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa     3180

gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag     3240

gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac     3300

tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc     3360

gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat     3420

caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat     3480

actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct     3540

acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt     3600

cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg     3660

gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta     3720

cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg     3780

gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg     3840

tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc     3900

tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg     3960

gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat     4020

aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc     4080

agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat     4140

ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca     4200

tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac     4260

acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca     4320

gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga     4380

aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa gcggcatgca taatgtgcct     4440

gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctccgtcaag ccgtcaattg     4500

tctgattcgt taccaattat gacaacttga cggctacatc attcactttt tcttcacaac     4560

cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaaataga     4620

gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca     4680

aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta     4740

actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc     4800

tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta     4860

cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca     4920

attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt     4980

taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga     5040

accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac     5100

gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa     5160

tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat     5220

ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg     5280

gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca     5340

gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt     5400

tcatactccc gccattcaga g                                               5421


<210>  10
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador directo con la secuencia que codifica para 10 histidinas 
       y para la diana de corte de la enterokinasa

<400>  10
gaagttctgt tccaggggcc ccatcaccat caccatcacc atcaccatca cgatgacgat       60

gacaagtttt ccagcatggg tagctatcca                                        90


<210>  11
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador inverso que hibrida con la secuencia que codifica para la
       diana de corte de pre-Scission

<400>  11
aaagctagcg ggcccctgga acagaacttc                                        30


<210>  12
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador directo para distinguir pPRETOP4-Tfam de pTOP-Tfam

<400>  12
ctaggctgga agttctgttc caggggcccg                                        30


<210>  13
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador inverso para distinguir pPRETOP4-Tfam de pTOP-Tfam

<400>  13
ttttgaattc ctctttatac ttgctcacag cttcttt                                37


<210>  14
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador directo para comprobar el vector pTOP-Tfam

<400>  14
gaccaaagcc atgacaaaaa cg                                                22


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador inverso para comprobar el vector pTOP-Tfam

<400>  15
tcgacggcgc tattcagatc                                                   20


<210>  16
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador directo de clonacin de PCBP1 en pTOP

<400>  16
cacgatgacg atgacaagga tgccggtgtg actgaa                                 36


<210>  17
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador inverso de clonacin de PCBP1 en pTOP

<400>  17
atgacaactc cgtcttccct agctgcaccc cat                                    33


<210>  18
<211>  5918
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Secuencia de pTOP-PCBP1

<400>  18
aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct       60

tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca      120

aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg      180

attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg      240

atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc      300

taacaggagg aattaaccat gggccaccac catcaccacc accatcatca tcatggcggc      360

agatcttccc ctatactagg ttattggaaa attaagggcc ttgtgcaacc cactcgactt      420

cttttggaat atcttgaaga aaaatatgaa gagcatttgt atgagcgcga tgaaggtgat      480

aaatggcgaa acaaaaagtt tgaattgggt ttggagtttc ccaatcttcc ttattatatt      540

gatggtgatg ttaaattaac acagtctatg gccatcatac gttatatagc tgacaagcac      600

aacatgttgg gtggttgtcc aaaagagcgt gcagagattt caatgcttga aggagcggtt      660

ttggatatta gatacggtgt ttcgagaatt gcatatagta aagactttga aactctcaaa      720

gttgattttc ttagcaagct acctgaaatg ctgaaaatgt tcgaagatcg tttatgtcat      780

aaaacatatt taaatggtga tcatgtaacc catcctgact tcatgttgta tgacgctctt      840

gatgttgttt tatacatgga cccaatgtgc ctggatgcgt tcccaaaatt agtttgtttt      900

aaaaaacgta ttgaagctat cccacaaatt gataagtact tgaaatccag caagtatata      960

gcatggcctt tgcagggctg gcaagccacg tttggtggtg gcgaccatcc tccaaaatcg     1020

gatctggaag ttctgttcca ggggccccat caccatcacc atcaccatca ccatcacgat     1080

gacgatgaca aggatgccgg tgtgactgaa agtggactaa atgtgactct caccattcgg     1140

cttcttatgc acggaaagga agtaggaagc atcattggga agaaagggga gtcggttaag     1200

aggatccgcg aggagagtgg cgcgcggatc aacatctcgg aggggaattg tccggagaga     1260

atcatcactc tgaccggccc caccaatgcc atctttaagg ctttcgctat gatcatcgac     1320

aagctggagg aagatatcaa cagctccatg accaacagta ccgcggccag caggcccccg     1380

gtcaccctga ggctggtggt gccggccacc cagtgcggct ccctgattgg gaaaggcggg     1440

tgtaagatca aagagatccg cgagagtacg ggggcgcagg tccaggtggc gggggatatg     1500

ctgcccaact ccaccgagcg ggccatcacc atcgctggcg tgccgcagtc tgtcaccgag     1560

tgtgtcaagc agatttgcct ggtcatgctg gagacgctct cccagtctcc gcaagggaga     1620

gtcatgacca ttccgtacca gcccatgccg gccagctccc cagtcatctg cgcgggcggc     1680

caagatcggt gcagcgacgc tgcgggctac ccccatgcca cccatgacct ggagggacca     1740

cctctagatg cctactcgat tcaaggacaa cacaccattt ctccgctcga tctggccaag     1800

ctgaaccagg tggcaagaca acagtctcac tttgccatga tgcacggcgg gaccggattc     1860

gccggaattg actccagctc tccagaggtg aaaggctatt gggcaagttt ggatgcatct     1920

actcaaacca cccatgaact caccattcca aataacttaa ttggctgcat aatcgggcgc     1980

caaggcgcca acattaatga gatccgccag atgtccgggg cccagatcaa aattgccaac     2040

ccagtggaag gctcctctgg taggcaggtt actatcactg gctctgctgc cagtattagt     2100

ctggcccagt atctaatcaa tgccaggctt tcctctgaga agggcatggg gtgcagctag     2160

ggaagacgga gttgtcattg gaattcgaag cttgggcccg aacaaaaact catctcagaa     2220

gaggatctga atagcgccgt cgaccatcat catcatcatc attgagttta aacggtctcc     2280

agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac     2340

gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg     2400

accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc     2460

atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg     2520

gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg     2580

ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca     2640

taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt     2700

ctacaaactc tttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac     2760

aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt     2820

tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag     2880

aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg     2940

aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa     3000

tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc     3060

aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag     3120

tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa     3180

ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc     3240

taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg     3300

agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa     3360

caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa     3420

tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg     3480

gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag     3540

cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg     3600

caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt     3660

ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt     3720

aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac     3780

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     3840

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     3900

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     3960

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     4020

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     4080

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     4140

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     4200

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     4260

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     4320

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     4380

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     4440

cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat     4500

cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca     4560

gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt     4620

attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa     4680

tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt     4740

catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct     4800

cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt     4860

ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga aggcgaagcg     4920

gcatgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc tatgctactc     4980

cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg ctacatcatt     5040

cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa     5100

tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg     5160

catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct     5220

taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca     5280

aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta     5340

ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc     5400

catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc     5460

ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc     5520

ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa gccattcatg     5580

ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg     5640

acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac     5700

aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata     5760

acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg     5820

cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg gatcattttg     5880

cgcttcagcc atacttttca tactcccgcc attcagag                             5918


<210>  19
<211>  5460
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Secuencia del plsmido pTOP-Tfam

<400>  19
aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct       60

tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca      120

aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg      180

attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg      240

atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg      300

ctaacaggag gaattaacca tgggccacca ccatcaccac caccatcatc atcatggcgg      360

cagatcttcc cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact      420

tcttttggaa tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga      480

taaatggcga aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat      540

tgatggtgat gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca      600

caacatgttg ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt      660

tttggatatt agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa      720

agttgatttt cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca      780

taaaacatat ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct      840

tgatgttgtt ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt      900

taaaaaacgt attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat      960

agcatggcct ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc     1020

ggatctggaa gttctgttcc aggggcccca tcaccatcac catcaccatc accatcacga     1080

tgacgatgac aagttttcca gcatgggtag ctatccaaag aaacctatga gttcatacct     1140

tcgattttcc acagaacagc tacccaaatt taaagctaaa cacccagatg caaaactttc     1200

agaattggtt aggaaaattg cagccctgtg gagggagcta ccagaagcag aaaaaaaggt     1260

ttatgaagct gattttaaag ctgagtggaa agcatacaaa gaagctgtga gcaagtataa     1320

agagcagcta actccaagtc agctgatggg tatggagaag gaggcccggc agagacggtt     1380

aaaaaagaaa gcactggtaa agagaagaga attaattttg cttggaaaac caaaaagacc     1440

tcgttcagca tataacattt atgtatctga aagcttccag gaggcaaagg atgattcggc     1500

tcagggaaaa ttgaagcttg taaatgaggc ttggaaaaat ctgtctcctg aggaaaagca     1560

ggcatatatt cagcttgcta aagatgatag gattcgttac gacaatgaaa tgaagtcttg     1620

ggaagagcag atggctgaag ttggacgaag tgatctcatc cgtcgaagtg tgaaacgatc     1680

cggagacatc tctgagcatt aaggaagacg gagttgtcat tggaattcga agcttgggcc     1740

cgaacaaaaa ctcatctcag aagaggatct gaatagcgcc gtcgaccatc atcatcatca     1800

tcattgagtt taaacggtct ccagcttggc tgttttggcg gatgagagaa gattttcagc     1860

ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc     1920

agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc     1980

gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg     2040

aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct     2100

cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg     2160

gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct     2220

gacggatggc ctttttgcgt ttctacaaac tctttttgtt tatttttcta aatacattca     2280

aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg     2340

aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc     2400

cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg     2460

ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt     2520

cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta     2580

ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat     2640

gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga     2700

gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca     2760

acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact     2820

cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc     2880

acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact     2940

ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt     3000

ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt     3060

gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt     3120

atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata     3180

ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag     3240

attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat     3300

ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa     3360

aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca     3420

aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt     3480

ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg     3540

tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc     3600

ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga     3660

cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc     3720

agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc     3780

gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca     3840

ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg     3900

tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta     3960

tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct     4020

cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag     4080

tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa     4140

gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc     4200

atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc     4260

cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg     4320

cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg     4380

ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagcagatca     4440

attcgcgcgc gaaggcgaag cggcatgcat aatgtgcctg tcaaatggac gaagcaggga     4500

ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg     4560

acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc     4620

tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat     4680

tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga     4740

tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg     4800

acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt     4860

ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat     4920

ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg     4980

ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt     5040

cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg     5100

acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat     5160

accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca     5220

aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc     5280

gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga     5340

gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc     5400

ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag     5460


<210>  20
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador directo de reamplificacin del inserto a clonar en pTOP 
       por CiPCR

<400>  20
tcaccatcac catcaccatc acgatgacga tgacaag                                37


<210>  21
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador reverso de reamplificacin del inserto a clonar en pTOP 
       por CiPCR

<400>  21
gcccaagctt cgaattccaa tgacaactcc gtcttcctta                             40


<210>  22
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Parte 5 del cebador directo para la clonacin del inserto por 
       CiPCR

<400>  22
cacgatgacg atgacaag                                                     18


<210>  23
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Parte 5 del cebador reverso de clonacin del inserto por CiPCR

<400>  23
atgacaactc cgtcttcctt a                                                 21


