                         SEQUENCE LISTING

<110>  Universidad de Santiago de Compostela
 
<120>  Nuevo mtodo de clonacin y mutagnesis in vitro mediante PCR 
       inversa de clonacin

<130>  PCT1596.30bis

<150>  ES201130222
<151>  2011-02-21

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador F para la clonacin de EGFP en pcDNA3

<400>  1
ccaaccctga ggaaccaatc acaaccatgg tgagcaa                                37


<210>  2
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador R para la clonacin de EGFP en pcDNA3

<400>  2
caatggcaag aaaggcatta cttgtacagc tcgtccatgc                             40


<210>  3
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador F para comprobar la clonacin de EGFP

<400>  3
ctgccgggct cccccaaccc tgaggaac                                          28


<210>  4
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador R para comprobar la clonacin de EGFP

<400>  4
ggccctctag agcacacaat ggcaag                                            26


<210>  5
<211>  7072
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plsmido pcDNA3-SLC16A2

<400>  5
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctttgcagc      900

agcagaaaca agtaccagcc acaaagcggc tcctctggcc caagcagcca cagtcccccc      960

gccgcgatgg cgctgcaaag ccaggcgagc gaggaagcaa aggggccctg gcaggaggca     1020

gaccaggaac agcaggagcc ggtgggtagc ccagagccgg agtctgagcc ggagcctgag     1080

cccgagcccg agcccgtgcc agtgcccccg cccgagcccc agccggagcc ccagccccta     1140

ccggaccccg cacccctgcc ggagctggag ttcgagtccg agcgggtgca cgaacccgag     1200

cccacgccta cggtagagac ccgcggcacc gcgcgcggct tccagcctcc cgaaggtggc     1260

ttcggctggg tggtggtgtt cgctgccacc tggtgcaacg gctccatctt cggcatccat     1320

aactctgtcg ggatcctcta ctccatgctg ctagaggagg aaaaggaaaa aaatcgccaa     1380

gtggagttcc aagcagcatg ggtcggagcc ctcgcgatgg gtatgatctt cttctgttct     1440

cccattgtga gtatattcac tgaccgtttg ggctgccgaa tcacagcaac cgcgggggct     1500

gccgttgctt tcattggcct ccataccagc tccttcacca gctccctaag cctgcgctac     1560

ttcacctacg ggattctctt tggttgtggc tgttccttcg cctttcagcc atccctcgtc     1620

atcctgggcc actactttca acgccgcctg ggtctggcca atggtgtggt gtctgctggg     1680

agtagcattt tctccatgtc cttccccttc ctcatcagaa tgctggggga taagatcaag     1740

ctggcccaaa ccttccaggt gctgagtacc ttcatgtttg ttcttatgct gctttcactc     1800

acctaccggc ccctcctgcc cagctcccag gacaccccaa gcaagagagg tgtccgcacc     1860

ctgcaccagc gctttctggc tcagctcagg aagtacttca acatgcgagt gttccgccaa     1920

cgcacttacc gcatctgggc cttcggaatt gctgctgctg cccttggcta ctttgttccc     1980

tatgtacacc tgatgaagta tgtggaggag gagttctcag aaatcaagga gacctgggtg     2040

ctcttggtgt gtattggggc tacctcaggc cttgggcgtc ttgtgtcagg ccacatcagt     2100

gactccatcc ctggacttaa gaagatctac ttgcaggtcc tttccttcct gctcctgggc     2160

ctgatgtcca tgatgattcc cctgtgccgg gacttcgggg gccttatcgt cgtctgtctt     2220

ttcctgggcc tttgcgatgg cttcttcatc accatcatgg cccccattgc atttgagctg     2280

gtgggcccaa tgcaggcctc acaggccatt ggctacctcc tgggcatgat ggccctgcca     2340

atgattgctg ggccccccat tgcaggccta ctccgcaact gttttgggga ctaccatgtg     2400

gccttctact ttgccggtgt gccccccatc atcggggctg taatcctctt cttcgtccct     2460

ctgatgcatc aaaggatgtt caagaaagag cagagagatt ccagcaagga taagatgttg     2520

gcccctgacc cagaccccaa tggggagcta ctgccgggct cccccaaccc tgaggaacca     2580

atctaatgcc tttcttgcca ttgtgtgctc tagagggccc tattctatag tgtcacctaa     2640

atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt     2700

gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat     2760

aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg     2820

tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg     2880

tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg     2940

cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta     3000

cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt     3060

tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg     3120

ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat     3180

cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac     3240

tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag     3300

ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg     3360

cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg     3420

caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc     3480

caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag     3540

tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc     3600

cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc     3660

tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg     3720

gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga     3780

ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct     3840

atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc     3900

aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg     3960

acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg     4020

acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc     4080

tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc     4140

ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg     4200

agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc     4260

atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg     4320

aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc     4380

gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag     4440

cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg     4500

tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg     4560

agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc     4620

atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt     4680

ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca     4740

ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt     4800

cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt     4860

atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata     4920

gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag     4980

cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg     5040

ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca     5100

acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc     5160

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg     5220

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa     5280

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     5340

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     5400

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     5460

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg     5520

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     5580

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     5640

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     5700

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac     5760

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     5820

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     5880

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc     5940

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt     6000

cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     6060

aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct     6120

atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg     6180

cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga     6240

tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt     6300

atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt     6360

taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt     6420

tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat     6480

gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc     6540

cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc     6600

cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat     6660

gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag     6720

aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt     6780

accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc     6840

ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa     6900

gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg     6960

aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa     7020

taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc             7072


<210>  6
<211>  7789
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Secuencia del plsmido pcDNA3-SLC16A2-EGFP

<400>  6
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctttgcagc      900

agcagaaaca agtaccagcc acaaagcggc tcctctggcc caagcagcca cagtcccccc      960

gccgcgatgg cgctgcaaag ccaggcgagc gaggaagcaa aggggccctg gcaggaggca     1020

gaccaggaac agcaggagcc ggtgggtagc ccagagccgg agtctgagcc ggagcctgag     1080

cccgagcccg agcccgtgcc agtgcccccg cccgagcccc agccggagcc ccagccccta     1140

ccggaccccg cacccctgcc ggagctggag ttcgagtccg agcgggtgca cgaacccgag     1200

cccacgccta cggtagagac ccgcggcacc gcgcgcggct tccagcctcc cgaaggtggc     1260

ttcggctggg tggtggtgtt cgctgccacc tggtgcaacg gctccatctt cggcatccat     1320

aactctgtcg ggatcctcta ctccatgctg ctagaggagg aaaaggaaaa aaatcgccaa     1380

gtggagttcc aagcagcatg ggtcggagcc ctcgcgatgg gtatgatctt cttctgttct     1440

cccattgtga gtatattcac tgaccgtttg ggctgccgaa tcacagcaac cgcgggggct     1500

gccgttgctt tcattggcct ccataccagc tccttcacca gctccctaag cctgcgctac     1560

ttcacctacg ggattctctt tggttgtggc tgttccttcg cctttcagcc atccctcgtc     1620

atcctgggcc actactttca acgccgcctg ggtctggcca atggtgtggt gtctgctggg     1680

agtagcattt tctccatgtc cttccccttc ctcatcagaa tgctggggga taagatcaag     1740

ctggcccaaa ccttccaggt gctgagtacc ttcatgtttg ttcttatgct gctttcactc     1800

acctaccggc ccctcctgcc cagctcccag gacaccccaa gcaagagagg tgtccgcacc     1860

ctgcaccagc gctttctggc tcagctcagg aagtacttca acatgcgagt gttccgccaa     1920

cgcacttacc gcatctgggc cttcggaatt gctgctgctg cccttggcta ctttgttccc     1980

tatgtacacc tgatgaagta tgtggaggag gagttctcag aaatcaagga gacctgggtg     2040

ctcttggtgt gtattggggc tacctcaggc cttgggcgtc ttgtgtcagg ccacatcagt     2100

gactccatcc ctggacttaa gaagatctac ttgcaggtcc tttccttcct gctcctgggc     2160

ctgatgtcca tgatgattcc cctgtgccgg gacttcgggg gccttatcgt cgtctgtctt     2220

ttcctgggcc tttgcgatgg cttcttcatc accatcatgg cccccattgc atttgagctg     2280

gtgggcccaa tgcaggcctc acaggccatt ggctacctcc tgggcatgat ggccctgcca     2340

atgattgctg ggccccccat tgcaggccta ctccgcaact gttttgggga ctaccatgtg     2400

gccttctact ttgccggtgt gccccccatc atcggggctg taatcctctt cttcgtccct     2460

ctgatgcatc aaaggatgtt caagaaagag cagagagatt ccagcaagga taagatgttg     2520

gcccctgacc cagaccccaa tggggagcta ctgccgggct cccccaaccc tgaggaacca     2580

atcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg     2640

gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc     2700

tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc     2760

accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg     2820

aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc     2880

ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc     2940

ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg     3000

cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag     3060

aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc     3120

gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac     3180

cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg     3240

gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag     3300

taatgccttt cttgccattg tgtgctctag agggccctat tctatagtgt cacctaaatg     3360

ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc     3420

cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa     3480

atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg     3540

ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg     3600

gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc     3660

cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac     3720

ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg     3780

ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt     3840

tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc     3900

cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct     3960

tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga     4020

ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga     4080

attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag     4140

gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag     4200

gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc     4260

cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc     4320

atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat     4380

tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag     4440

cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg     4500

aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg     4560

actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg     4620

ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg     4680

aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg     4740

ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc     4800

tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc     4860

tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc     4920

gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc     4980

aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg     5040

atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct     5100

tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt     5160

tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc     5220

tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt     5280

tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc     5340

acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg     5400

ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc     5460

caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac     5520

aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc     5580

ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct     5640

gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat     5700

aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc     5760

actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg     5820

cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct     5880

gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt     5940

atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc     6000

caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga     6060

gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata     6120

ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac     6180

cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg     6240

taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc     6300

cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag     6360

acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt     6420

aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt     6480

atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg     6540

atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac     6600

gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca     6660

gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac     6720

ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac     6780

ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt     6840

tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt     6900

accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt     6960

atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc     7020

cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa     7080

tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg     7140

tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt     7200

gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc     7260

agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt     7320

aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg     7380

gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac     7440

tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc     7500

gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt     7560

tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg     7620

aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag     7680

catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa     7740

acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc                 7789


<210>  7
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador F para la clonacin y mutagnesis de PCBP1 en pcDNA3

<400>  7
cactataggg agacccaagc tcgccatgga tgccggtgtg actg                        44


<210>  8
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador R para la clonacin y mutagnesis de PCBP1 en pcDNA3

<400>  8
agctcctcgc ccttgctcac catctagctg caccccatg                              39


<210>  9
<211>  7174
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Secuencia del plsmido pcDNA3-PCBP1

<400>  9
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctcgccatg      900

gatgccggtg tgactgaaag tggactaaat gtgactctca ccattcggct tcttatgcac      960

ggaaaggaag taggaagcat cattgggaag aaaggggagt cggttaagag gatccgcgag     1020

gagagtggcg cgcggatcaa catctcggag gggaattgtc cggagagaat catcactctg     1080

accggcccca ccaatgccat ctttaaggct ttcgctatga tcatcgacaa gctggaggaa     1140

gatatcaaca gctccatgac caacagtacc gcggccagca ggcccccggt caccctgagg     1200

ctggtggtgc cggccaccca gtgcggctcc ctgattggga aaggcgggtg taagatcaaa     1260

gagatccgcg agagtacggg ggcgcaggtc caggtggcgg gggatatgct gcccaactcc     1320

accgagcggg ccatcaccat cgctggcgtg ccgcagtctg tcaccgagtg tgtcaagcag     1380

atttgcctgg tcatgctgga gacgctctcc cagtctccgc aagggagagt catgaccatt     1440

ccgtaccagc ccatgccggc cagctcccca gtcatctgcg cgggcggcca agatcggtgc     1500

agcgacgctg cgggctaccc ccatgccacc catgacctgg agggaccacc tctagatgcc     1560

tactcgattc aaggacaaca caccatttct ccgctcgatc tggccaagct gaaccaggtg     1620

gcaagacaac agtctcactt tgccatgatg cacggcggga ccggattcgc cggaattgac     1680

tccagctctc cagaggtgaa aggctattgg gcaagtttgg atgcatctac tcaaaccacc     1740

catgaactca ccattccaaa taacttaatt ggctgcataa tcgggcgcca aggcgccaac     1800

attaatgaga tccgccagat gtccggggcc cagatcaaaa ttgccaaccc agtggaaggc     1860

tcctctggta ggcaggttac tatcactggc tctgctgcca gtattagtct ggcccagtat     1920

ctaatcaatg ccaggctttc ctctgagaag ggcatggggt gcagctagat ggtgagcaag     1980

ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac     2040

ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc     2100

ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc     2160

ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc     2220

ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac     2280

ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc     2340

gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac     2400

aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg     2460

aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag     2520

cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc     2580

cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc     2640

gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaatg cctttcttgc     2700

cattgtgtgc tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat     2760

cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt     2820

ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat     2880

cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg     2940

gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg     3000

aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat     3060

taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag     3120

cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc     3180

aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc     3240

ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt     3300

ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa     3360

caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg     3420

cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa     3480

tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa     3540

gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca     3600

gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc     3660

ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt     3720

tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag     3780

gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt     3840

tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc     3900

acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga     3960

caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt     4020

ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat     4080

cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg     4140

gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg     4200

ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc     4260

cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga     4320

tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag     4380

ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc     4440

atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg     4500

actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata     4560

ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg     4620

ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac     4680

tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc     4740

caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat     4800

gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc     4860

agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt     4920

ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat     4980

accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa     5040

ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg     5100

gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca     5160

gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg     5220

tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg     5280

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg     5340

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa     5400

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg     5460

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc     5520

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc     5580

ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc     5640

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg     5700

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc     5760

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga     5820

gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc     5880

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac     5940

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg     6000

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc     6060

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa     6120

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta     6180

ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt     6240

tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag     6300

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca     6360

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc     6420

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt     6480

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag     6540

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt     6600

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat     6660

ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt     6720

gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc     6780

ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat     6840

cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag     6900

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt     6960

ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg     7020

gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta     7080

ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc     7140

gcgcacattt ccccgaaaag tgccacctga cgtc                                 7174


<210>  10
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador F para la clonacin de SP1 en pcDNA3

<400>  10
cactataggg agacccaagc ccaccatgag cgaccaagat cactccat                    48


<210>  11
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador R para la clonacin de SP1 en pcDNA3

<400>  11
agctcctcgc ccttgctcac cattcagaag ccattgccac t                           41


<210>  12
<211>  8461
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Secuencia del plsmido pcDNA3-SP1

<400>  12
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcccaccatg      900

agcgaccaag atcactccat ggatgaaatg acagctgtgg tgaaaattga aaaaggagtt      960

ggtggcaata atgggggcaa tggtaatggt ggtggtgcct tttcacaggc tcgaagtagc     1020

agcacaggca gtagcagcag cactggagga ggagggcagg agtcccagcc atcccctttg     1080

gctctgctgg cagcaacttg cagcagaatt gagtcaccca atgagaacag caacaactcc     1140

cagggcccga gtcagtcagg gggaacaggt gagcttgacc tcacagccac acaactttca     1200

cagggtgcca atggctggca gatcatctct tcctcctctg gggctacccc tacctcaaag     1260

gaacagagtg gcagcagtac caatggcagc aatggcagtg agtcttccaa gaatcgcaca     1320

gtctctggtg ggcagtatgt tgtggctgcc gctcccaact tacagaacca gcaagttctg     1380

acaggactac ctggagtgat gcctaatatt cagtatcaag taatcccaca gttccagacc     1440

gttgatgggc aacagctgca gtttgctgcc actggggccc aagtgcagca ggatggttct     1500

ggtcaaatac agatcatacc aggtgcaaac caacagatta tcacaaatcg aggaagtgga     1560

ggcaacatca ttgctgctat gccaaaccta ctccagcagg ctgtccccct ccaaggcctg     1620

gctaataatg tactctcagg acagactcag tatgtgacca atgtaccagt ggccctgaat     1680

gggaacatca ccttgctacc tgtcaacagc gtttctgcag ctaccttgac tcccagctct     1740

caggcagtca cgatcagcag ctctgggtcc caggagagtg gctcacagcc tgtcacctca     1800

gggactacca tcagttctgc cagcttggta tcatcacaag ccagttccag ctcctttttc     1860

accaatgcca atagctactc aactactact accaccagca acatgggaat tatgaacttt     1920

actaccagtg gatcatcagg gaccaactct caaggccaga caccccagag ggtcagtggg     1980

ctacaggggt ctgatgctct gaacatccag caaaaccaga catctggagg ctcattgcaa     2040

gcaggccagc aaaaagaagg agagcaaaac cagcagacac agcagcaaca aattcttatc     2100

cagcctcagc tagttcaagg gggacaggcc ctccaggccc tccaagcagc accattgtca     2160

gggcagacct ttacaactca agccatctcc caggaaaccc tccagaacct ccagcttcag     2220

gctgttccaa actctggtcc catcatcatc cggacaccaa cagtggggcc caatggacag     2280

gtcagttggc agactctaca gctgcagaac ctccaagttc agaacccaca agcccaaaca     2340

atcaccttag ccccaatgca gggtgtttcc ttggggcaga ccagcagcag caacaccact     2400

ctcacaccca ttgcctcagc tgcttccatt cctgctggca cagtcactgt gaatgctgct     2460

caactctcct ccatgccagg cctccagacc attaacctca gtgcattggg tacttcagga     2520

atccaggtgc acccaattca aggcctgccg ttggctatag caaatgcccc aggtgatcat     2580

ggagctcagc ttggtctcca tggggctggt ggtgatggaa tacatgatga cacagcaggt     2640

ggagaggaag gagaaaacag cccagatgcc caaccccaag ccggtcggag gacccggcgg     2700

gaagcatgca cctgccccta ctgtaaagac agtgaaggaa ggggctcggg ggatcctggc     2760

aaaaagaaac agcatatttg ccacatccaa ggctgtggga aagtgtatgg caagacctct     2820

cacctgcggg cacacttgcg ctggcataca ggcgagaggc catttatgtg tacctggtca     2880

tactgtggga aacgcttcac acgttcggat gagctacaga ggcacaaacg tacacacaca     2940

ggtgagaaga aatttgcctg ccctgagtgt cctaagcgct tcatgaggag tgaccacctg     3000

tcaaaacata tcaagaccca ccagaataag aagggaggcc caggtgtagc tctgagtgtg     3060

ggcactttgc ccctggacag tggggcaggt tcagaaggca gtggcactgc cactccttca     3120

gcccttatta ccaccaatat ggtagccatg gaggccatct gtccagaggg cattgcccgt     3180

cttgccaaca gtggcatcaa cgtcatgcag gtggcagatc tgcagtccat taatatcagt     3240

ggcaatggct tctgaatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc     3300

ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag     3360

ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc     3420

gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac     3480

cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag     3540

gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc     3600

gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc     3660

aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc     3720

gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc     3780

agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg     3840

ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag     3900

cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac     3960

gagctgtaca agtaatgcct ttcttgccat tgtgtgctct agagggccct attctatagt     4020

gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat     4080

ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc     4140

tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg     4200

ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg     4260

gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt     4320

atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg     4380

tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc     4440

tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc     4500

gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta     4560

gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta     4620

atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg     4680

atttataagg gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa     4740

aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg     4800

ctccccaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg     4860

gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag     4920

caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc     4980

attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg     5040

cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa     5100

agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt     5160

ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc     5220

tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc     5280

tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg     5340

aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag     5400

ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg     5460

ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg     5520

caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac     5580

atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg     5640

acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc     5700

ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg     5760

aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc     5820

aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc     5880

gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc     5940

ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc     6000

caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg     6060

aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt     6120

cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat     6180

cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact     6240

catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc     6300

atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg     6360

agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat     6420

tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg     6480

aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct     6540

cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc     6600

ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg     6660

ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg     6720

cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg     6780

actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac     6840

cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca     6900

atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt     6960

gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc     7020

caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag     7080

agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac     7140

tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt     7200

tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa     7260

gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg     7320

gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa     7380

aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat     7440

atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc     7500

gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat     7560

acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc     7620

ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc     7680

tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag     7740

ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg     7800

ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg     7860

atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag     7920

taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt     7980

catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga     8040

atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc     8100

acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc     8160

aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc     8220

ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc     8280

cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca     8340

atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat     8400

ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt     8460

c                                                                     8461


<210>  13
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador F1 para la PCRc1 de la clonacin por CiPCR lineal del 
       ejemplo 4.

<400>  13
taatacgact cactataggg ctcagaagtc cagaatccag                             40


<210>  14
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador R1 para la PCRc1 de la clonacin por CiPCR lineal del 
       ejemplo 4.

<400>  14
gctatttagg tgacactata gagaatttga ttgactttcc c                           41


<210>  15
<211>  500
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplicn resultante de la PCRc1 del ejemplo 4.

<400>  15
taatacgact cactataggg ctcagaagtc cagaatccag acagccaagg gataagcagc       60

cagagagctt gtcatggagg tagggctaca ggagaggggg tttctgtcct gctccaggaa      120

agtaagcagt aggggattct ggatatgcct acagttaacc agtcagctcc tctttctcct      180

cctgtttctg gggatccttg tgctatgctt ttcagatgaa gtatgtggag gaggagttct      240

cagaaatcaa ggagacctgg gtgctcttgg tgtgtattgg ggctacctca ggccttgggc      300

gtcttgtgtc aggccacatc agtgactcca tccctggact taagaagatc tacttgcagg      360

tgagtgtgac cacttgtcca ctgtggggag aaacagcctg ccacaaatgc aaggttatcc      420

acagcctttg ggatagaatg gtagcttgtt gtgtcgcatg ggaaagtcaa tcaaattctc      480

tatagtgtca cctaaatagc                                                  500


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador F2 para la PCRic2 de la clonacin por CiPCR lineal del 
       ejemplo 4.

<400>  16
ctatagtgtc acctaaatag c                                                 21


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador R2 para la PCRic2 de la clonacin por CiPCR lineal del 
       ejemplo 4.

<400>  17
ccctatagtg agtcgtatta                                                   20


<210>  18
<211>  3337
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Secuencia de pGEM-T Easy-Exon4-SLC16A2.

<400>  18
taatacgact cactataggg ctcagaagtc cagaatccag acagccaagg gataagcagc       60

cagagagctt gtcatggagg tagggctaca ggagaggggg tttctgtcct gctccaggaa      120

agtaagcagt aggggattct ggatatgcct acagttaacc agtcagctcc tctttctcct      180

cctgtttctg gggatccttg tgctatgctt ttcagatgaa gtatgtggag gaggagttct      240

cagaaatcaa ggagacctgg gtgctcttgg tgtgtattgg ggctacctca ggccttgggc      300

gtcttgtgtc aggccacatc agtgactcca tccctggact taagaagatc tacttgcagg      360

tgagtgtgac cacttgtcca ctgtggggag aaacagcctg ccacaaatgc aaggttatcc      420

acagcctttg ggatagaatg gtagcttgtt gtgtcgcatg ggaaagtcaa tcaaattctc      480

tatagtgtca cctaaatagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt      540

gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg      600

gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt      660

cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt      720

tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc      780

tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg      840

ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg      900

ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac      960

gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg     1020

gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct     1080

ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg     1140

tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct     1200

gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac     1260

tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt     1320

tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc     1380

tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca     1440

ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat     1500

ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac     1560

gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt     1620

aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc     1680

aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg     1740

cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg     1800

ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc     1860

cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta     1920

ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg     1980

ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct     2040

ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta     2100

gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg     2160

ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga     2220

ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt     2280

gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca     2340

ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt     2400

cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt     2460

ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga     2520

aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt     2580

gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc     2640

gcacatttcc ccgaaaagtg ccacctgatg cggtgtgaaa taccgcacag atgcgtaagg     2700

agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt     2760

tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat     2820

caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat     2880

taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac     2940

tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc     3000

ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga     3060

gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca     3120

cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc     3180

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     3240

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3300

ccagtcacga cgttgtaaaa cgacggccag tgaattg                              3337


<210>  19
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador F1 para la PCRc1 de la clonacin por CiPCR lineal del 
       ejemplo 5.

<400>  19
ccaaccctga ggaaccaatc acaaccatgg tgagcaa                                37


<210>  20
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador R1 para la PCRc1 de la clonacin por CiPCR lineal del 
       ejemplo 5.

<400>  20
caatggcaag aaaggcatta cttgtacagc tcgtccatgc                             40


<210>  21
<211>  763
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amplicn resultante de la PCRc1 del ejemplo 5.

<400>  21
ccaaccctga ggaaccaatc acaaccatgg tgagcaaggg cgaggagctg ttcaccgggg       60

tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg      120

gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg      180

gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct      240

tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag      300

gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg      360

aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca      420

aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct      480

atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca      540

tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg      600

gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc      660

ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc      720

tcggcatgga cgagctgtac aagtaatgcc tttcttgcca ttg                        763


<210>  22
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador F2 para la PCRic2 de la clonacin por CiPCR lineal del 
       ejemplo 5.

<400>  22
atgcctttct tgccattgtg tgc                                               23


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador R2 para la PCRic2 de la clonacin por CiPCR lineal del 
       ejemplo 5.

<400>  23
gattggttcc tcagggttgg                                                   20


<210>  24
<211>  9034
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Secuencia de pcDNA3-DELTACMV+Pr1743SLC16A2-SLC16A2-EGFP.

<400>  24
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttggt gaaacacagg gagcatgaag cttctcgtta tatggagacc      240

tgccttgaat tctagcttgt ccttgacttg gcccatgatc ttaggctaat aaatgccttg      300

acctcgttga gcctcagttt tcgtgcaaaa aagcaggtaa taataaacct tagggcaata      360

agggacgtgc tctgttcggg caatgccttc cctgctgagt ggcgtggccc tgtgattttt      420

gaatttggct gattcctctc atatggcagc gcaagcccct caggacgtct atatccttcc      480

acccagctcc gccagccctc aggtgcttaa tgactgtatt tacaggcatc tagcgcagcc      540

acgcagcccc ggagctgcat cacctctggc tatgcagtct ttcacttatc aggagggtta      600

atgagatgca gggtagaaag gtcttgccac tcgcccgccc gctcgtggaa gccgagtagc      660

ttggtaacga caccacgact tcacgtttag tgcattagac gcgtagaccc ttaggggagg      720

cagagcttcc cggtatcgcc gagcgggccc tgagggctga ggctgggcac ccctgcgcac      780

ccctgctgag tgcccggcca ccagaaagac agatccccca cctttccttc tccctgaccc      840

ggcgcgcttc ctggggaaca aactctccct taccctcagt ggggctgggg cctcagtctg      900

cgacatacag tgggactccc atgtctgcat gcctgaggtg ggcggaaggg ggcggtgggt      960

gctgggaaga catccgtccg cttgcagctc cgctgagtct ccagcacctg aaaagccgcg     1020

gccgaggcct gagggaccag cccagccgag atatcggggg cggggcagcc caatcccaaa     1080

ctggcggctg gacccgactc caactcgttc ctcctaagtc cgggacagat tgtcgggagg     1140

agcttgaact gagcttcggc tcccgactgg gcctgggccc ggcccccact ggtgccacct     1200

ccctcccttc ctggcccggc tcctggccca cactcggccc tggcccggcc ctggcccggc     1260

ccagacccgg cccggccctg gcccggccca gacccggccc tctgaggctc ctccgggctc     1320

caggttggcg caaacaaagc cctgattgac tgcccggagg ggcgggcctc gctgcatggc     1380

cgcctcctcc cctcccaggc cgccagggat ttgccgggaa gagcgggggc agggggagac     1440

tcccgggggt gcgcaggacc gcggggggcc gagtcctcgg gctcgagaag attgtgtcca     1500

cccgctgtcc gaggcccagg ctgtcccctc aggtcgtcct cctcaagtcc aaggagggac     1560

atgggagctc ctgagggcag ggtggggttc ctcgcgggcc agctgtcgtc ctagggtccc     1620

cggtgccacc tcagttgcgg gctgcttgtg aggaaggagg gtatgggagc aaaaaatagg     1680

aggggtcttc ccgaggctcg gggttgatca gtgtcggttc tagagcctgc ggggaaaaga     1740

gggcaggaga acaggatagt ttgtgcttcc ttttttccat tttaactttt cctctaacgg     1800

gcatccgggc aagaacaggg ttcagtagtt tgggaaggga gctgggggag gcagtggcgg     1860

ctggagtagg taggcgagta aaaggaggag gaagggctgg ggagaagagc gaggctgtag     1920

cggctgcctg ttgagggagg aagaggtggg gggtgtggag gagggggagg agagagagat     1980

gacatgggga gaggaggagg ggggttggac gtgggaggag gaggagaggg ctcgagggac     2040

cgtctgtcgc gggacgggct ggccagctgg ggcgcggagc ctggaggagg aggcagcggc     2100

agcggcagca gcagccctcc gagcagcagc agctgcagca gcagaaacaa gtaccagcca     2160

caaagcggct cctctggccc aagcagccac agtccccccg ccgcgatggc gctgcaaagc     2220

caggcgagcg aggaagcaaa ggggccctgg caggaggcag accaggaaca gcaggagccg     2280

gtgggtagcc cagagccgga gtctgagccg gagcctgagc ccgagcccga gcccgtgcca     2340

gtgcccccgc ccgagcccca gccggagccc cagcccctac cggaccccgc acccctgccg     2400

gagctggagt tcgagtccga gcgggtgcac gaacccgagc ccacgcctac ggtagagacc     2460

cgcggcaccg cgcgcggctt ccagcctccc gaaggtggct tcggctgggt ggtggtgttc     2520

gctgccacct ggtgcaacgg ctccatcttc ggcatccata actctgtcgg gatcctctac     2580

tccatgctgc tagaggagga aaaggaaaaa aatcgccaag tggagttcca agcagcatgg     2640

gtcggagccc tcgcgatggg tatgatcttc ttctgttctc ccattgtgag tatattcact     2700

gaccgtttgg gctgccgaat cacagcaacc gcgggggctg ccgttgcttt cattggcctc     2760

cataccagct ccttcaccag ctccctaagc ctgcgctact tcacctacgg gattctcttt     2820

ggttgtggct gttccttcgc ctttcagcca tccctcgtca tcctgggcca ctactttcaa     2880

cgccgcctgg gtctggccaa tggtgtggtg tctgctggga gtagcatttt ctccatgtcc     2940

ttccccttcc tcatcagaat gctgggggat aagatcaagc tggcccaaac cttccaggtg     3000

ctgagtacct tcatgtttgt tcttatgctg ctttcactca cctaccggcc cctcctgccc     3060

agctcccagg acaccccaag caagagaggt gtccgcaccc tgcaccagcg ctttctggct     3120

cagctcagga agtacttcaa catgcgagtg ttccgccaac gcacttaccg catctgggcc     3180

ttcggaattg ctgctgctgc ccttggctac tttgttccct atgtacacct gatgaagtat     3240

gtggaggagg agttctcaga aatcaaggag acctgggtgc tcttggtgtg tattggggct     3300

acctcaggcc ttgggcgtct tgtgtcaggc cacatcagtg actccatccc tggacttaag     3360

aagatctact tgcaggtcct ttccttcctg ctcctgggcc tgatgtccat gatgattccc     3420

ctgtgccggg acttcggggg ccttatcgtc gtctgtcttt tcctgggcct ttgcgatggc     3480

ttcttcatca ccatcatggc ccccattgca tttgagctgg tgggcccaat gcaggcctca     3540

caggccattg gctacctcct gggcatgatg gccctgccaa tgattgctgg gccccccatt     3600

gcaggcctac tccgcaactg ttttggggac taccatgtgg ccttctactt tgccggtgtg     3660

ccccccatca tcggggctgt aatcctcttc ttcgtccctc tgatgcatca aaggatgttc     3720

aagaaagagc agagagattc cagcaaggat aagatgttgg cccctgaccc agaccccaat     3780

ggggagctac tgccgggctc ccccaaccct gaggaaccaa tcacaaccat ggtgagcaag     3840

ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac     3900

ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc     3960

ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc     4020

ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc     4080

ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac     4140

ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc     4200

gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac     4260

aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg     4320

aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag     4380

cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc     4440

cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc     4500

gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaatg cctttcttgc     4560

cattgtgtgc tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat     4620

cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt     4680

ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat     4740

cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg     4800

gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg     4860

aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat     4920

taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag     4980

cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc     5040

aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc     5100

ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt     5160

ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa     5220

caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg     5280

cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa     5340

tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa     5400

gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca     5460

gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc     5520

ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt     5580

tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag     5640

gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt     5700

tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc     5760

acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga     5820

caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt     5880

ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat     5940

cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg     6000

gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg     6060

ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc     6120

cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga     6180

tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag     6240

ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc     6300

atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg     6360

actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata     6420

ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg     6480

ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac     6540

tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc     6600

caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat     6660

gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc     6720

agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt     6780

ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat     6840

accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa     6900

ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg     6960

gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca     7020

gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg     7080

tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg     7140

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg     7200

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa     7260

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg     7320

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc     7380

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc     7440

ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc     7500

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg     7560

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc     7620

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga     7680

gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc     7740

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac     7800

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg     7860

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc     7920

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa     7980

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta     8040

ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt     8100

tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag     8160

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca     8220

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc     8280

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt     8340

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag     8400

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt     8460

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat     8520

ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt     8580

gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc     8640

ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat     8700

cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag     8760

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt     8820

ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg     8880

gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta     8940

ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc     9000

gcgcacattt ccccgaaaag tgccacctga cgtc                                 9034


