                         SEQUENCE LISTING

<110>  Laboratorios del Dr. Esteve S.A.
       Fundaci Privada Institut de Recerca de la SIDA-Caixa
 
<120>  RAPID IMMUNOGEN SELECTION METHOD

<130>  P6927PC00

<150>  EP 11382051
<151>  2011-02-25

<150>  US 61/446,595
<151>  2011-02-25

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  2583
<212>  DNA
<213>  Artificial

<220>
<223>  env gene of the HIV AC10 isolate

<400>  1
atgagagtga gggagaccag gaagaattat cagcacttgt ggtggaaatg gggcatgatg       60

ctccttggga tgttgatgat ctgtagtgct gtagaacaaa cgtgggtcac agtctattat      120

ggggtacctg tgtggaaaga agcaaacacc attttatttt gtgcatcaga tgctaaagca      180

tataatacag aggtacataa tgtttgggcc acacatgcct gtgtacccac agaccccaac      240

ccacaagaag tagaattgga aaatgtgaca gaaaatttta acatgtggaa aaacaacatg      300

gtagatcaga tgcatgggga tataatcagt ttatgggatc aaagcctaaa gccatgtgta      360

aaattaactc cactctgtgt tactttaagt tgcactgata atgtgggaaa tgatactagt      420

accaataata gtagatggga taaaatggaa aaaggagaaa taaagaactg ctctttcaat      480

atcaccacaa acatgagaga taagatgcag aaacaatatg cactttttta taaacttgat      540

gtagtaccaa tagaggaagg taagaataat aacagtagtt ttaccgacta taggttgata      600

agttgtaaca cctcagtcat tacacaggcc tgtccaaagg taacctttga gccaattccc      660

atacattatt gtgccccagc tggttttgcg cttctaaaat gtaaggataa gaaattcaat      720

ggaacaggac catgtaaaaa tgttagcaca gtacaatgta cacatggaat taagccagta      780

gtatcaactc agctgctatt aaatggcagt ctagcagaag aagaggtagt aataagatct      840

gagaacttct cgaacaatgc tagaaccata atagtacagc tgaatacatc tgtagaaata      900

aagtgtataa gacccaacaa caatacaaga aaaggtatac atataggacc agggagagca      960

ttttatacaa caggagatat aataggagat ataagacaag cacattgtaa cattagtaga     1020

caaaattgga ataacacttt aaaacagata gctgaaaagt taagagaaca atttgggaat     1080

aaaacaatag tctttagaaa ctcctctgga ggggatccag aaattgtaat gcacactttt     1140

aattgtgcag gggaattttt ctactgtaat acagcagaac tgtttaatag tacttggtat     1200

gcaaatggca caattagtat tggaggggga aacaagacta atatcatact cccatgcaga     1260

ataaaacaat ttataaacat gtggcaggaa gtaggaaaag caatgtatgc ccctcccatc     1320

agtggacaga ttagatgttc atcaaatatt acaggactgc tattaacaag agatggtggt     1380

aggggcaatc agaccgacaa ccagactgag atcttcagac ctgtaggagg agatatgaaa     1440

aacaattgga gaagtgaatt atataaatat aaagtagtaa gaattgaacc attaggaata     1500

gcacccacca gggcaaaaag gagagtggtg cagagagaaa aaagagcagt gggaatagga     1560

gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     1620

ctgacggtac aggccagact attattgtct ggtatagtgc aacagcagaa caatctgctg     1680

agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     1740

caggcaagag tcctggctgt ggaaagatac ctacgtgatc aacagctcct gggaatttgg     1800

ggttgctctg gaaaactcat ctgcaccact gctgtgcctt ggaatgttag ttggaataat     1860

agatctgtgg atgacatttg ggaaaacatg acctggatgc agtgggatag agaaattagt     1920

aattacacaa gtttaatata caccttaatt gaagaatcgc agaatcagca agaaaagaat     1980

gaacaagaat tattggcatt agataaatgg gcaaatttgt ggaattggtt caacataaca     2040

gaatggctgt ggtatataaa aatattcata atgatagtag gaggcttggt aggtttaaga     2100

atagtttttg ctgtgctttc tatagtgaat agagttaggc agggatactc accattatcg     2160

tttcagaccc acctcccagc tcagagggga cccgacaggc ccggaggaat cgaagaagaa     2220

ggtggagaga gcgacagaga cagatccgga agattagtga atggattctt agcaattatc     2280

tggatcgacc tgcggagcct gtgccttttc agctaccacc acttgagaga cttactattg     2340

attgtaacga ggattgtgga aattctggga cgcagggggt gggaaatcct caagtattgg     2400

tggaatctcc tgcagtattg gattcaggaa ctaaagaata gtgctgttag cttgctcaac     2460

gccatagcca tagcagtagg tgaggggaca gataggatta tagaagcatt tagaagcatt     2520

tttagagcta ttctccacat acctacaaga ataagacagg gcttggaaag gtctttgcta     2580

taa                                                                   2583


<210>  2
<211>  860
<212>  PRT
<213>  Artificial

<220>
<223>  env polypeptide of the HIV AC10 isolate

<400>  2

Met Arg Val Arg Glu Thr Arg Lys Asn Tyr Gln His Leu Trp Trp Lys 
1               5                   10                  15      


Trp Gly Met Met Leu Leu Gly Met Leu Met Ile Cys Ser Ala Val Glu 
            20                  25                  30          


Gln Thr Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
        35                  40                  45              


Asn Thr Ile Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asn Thr Glu 
    50                  55                  60                  


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
65                  70                  75                  80  


Pro Gln Glu Val Glu Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp 
                85                  90                  95      


Lys Asn Asn Met Val Asp Gln Met His Gly Asp Ile Ile Ser Leu Trp 
            100                 105                 110         


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
        115                 120                 125             


Leu Ser Cys Thr Asp Asn Val Gly Asn Asp Thr Ser Thr Asn Asn Ser 
    130                 135                 140                 


Arg Trp Asp Lys Met Glu Lys Gly Glu Ile Lys Asn Cys Ser Phe Asn 
145                 150                 155                 160 


Ile Thr Thr Asn Met Arg Asp Lys Met Gln Lys Gln Tyr Ala Leu Phe 
                165                 170                 175     


Tyr Lys Leu Asp Val Val Pro Ile Glu Glu Gly Lys Asn Asn Asn Ser 
            180                 185                 190         


Ser Phe Thr Asp Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr 
        195                 200                 205             


Gln Ala Cys Pro Lys Val Thr Phe Glu Pro Ile Pro Ile His Tyr Cys 
    210                 215                 220                 


Ala Pro Ala Gly Phe Ala Leu Leu Lys Cys Lys Asp Lys Lys Phe Asn 
225                 230                 235                 240 


Gly Thr Gly Pro Cys Lys Asn Val Ser Thr Val Gln Cys Thr His Gly 
                245                 250                 255     


Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala 
            260                 265                 270         


Glu Glu Glu Val Val Ile Arg Ser Glu Asn Phe Ser Asn Asn Ala Arg 
        275                 280                 285             


Thr Ile Ile Val Gln Leu Asn Thr Ser Val Glu Ile Lys Cys Ile Arg 
    290                 295                 300                 


Pro Asn Asn Asn Thr Arg Lys Gly Ile His Ile Gly Pro Gly Arg Ala 
305                 310                 315                 320 


Phe Tyr Thr Thr Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys 
                325                 330                 335     


Asn Ile Ser Arg Gln Asn Trp Asn Asn Thr Leu Lys Gln Ile Ala Glu 
            340                 345                 350         


Lys Leu Arg Glu Gln Phe Gly Asn Lys Thr Ile Val Phe Arg Asn Ser 
        355                 360                 365             


Ser Gly Gly Asp Pro Glu Ile Val Met His Thr Phe Asn Cys Ala Gly 
    370                 375                 380                 


Glu Phe Phe Tyr Cys Asn Thr Ala Glu Leu Phe Asn Ser Thr Trp Tyr 
385                 390                 395                 400 


Ala Asn Gly Thr Ile Ser Ile Gly Gly Gly Asn Lys Thr Asn Ile Ile 
                405                 410                 415     


Leu Pro Cys Arg Ile Lys Gln Phe Ile Asn Met Trp Gln Glu Val Gly 
            420                 425                 430         


Lys Ala Met Tyr Ala Pro Pro Ile Ser Gly Gln Ile Arg Cys Ser Ser 
        435                 440                 445             


Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Arg Gly Asn Gln 
    450                 455                 460                 


Thr Asp Asn Gln Thr Glu Ile Phe Arg Pro Val Gly Gly Asp Met Lys 
465                 470                 475                 480 


Asn Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Arg Ile Glu 
                485                 490                 495     


Pro Leu Gly Ile Ala Pro Thr Arg Ala Lys Arg Arg Val Val Gln Arg 
            500                 505                 510         


Glu Lys Arg Ala Val Gly Ile Gly Ala Leu Phe Leu Gly Phe Leu Gly 
        515                 520                 525             


Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Gln 
    530                 535                 540                 


Ala Arg Leu Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu 
545                 550                 555                 560 


Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly 
                565                 570                 575     


Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Arg 
            580                 585                 590         


Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys 
        595                 600                 605             


Thr Thr Ala Val Pro Trp Asn Val Ser Trp Asn Asn Arg Ser Val Asp 
    610                 615                 620                 


Asp Ile Trp Glu Asn Met Thr Trp Met Gln Trp Asp Arg Glu Ile Ser 
625                 630                 635                 640 


Asn Tyr Thr Ser Leu Ile Tyr Thr Leu Ile Glu Glu Ser Gln Asn Gln 
                645                 650                 655     


Gln Glu Lys Asn Glu Gln Glu Leu Leu Ala Leu Asp Lys Trp Ala Asn 
            660                 665                 670         


Leu Trp Asn Trp Phe Asn Ile Thr Glu Trp Leu Trp Tyr Ile Lys Ile 
        675                 680                 685             


Phe Ile Met Ile Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala 
    690                 695                 700                 


Val Leu Ser Ile Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser 
705                 710                 715                 720 


Phe Gln Thr His Leu Pro Ala Gln Arg Gly Pro Asp Arg Pro Gly Gly 
                725                 730                 735     


Ile Glu Glu Glu Gly Gly Glu Ser Asp Arg Asp Arg Ser Gly Arg Leu 
            740                 745                 750         


Val Asn Gly Phe Leu Ala Ile Ile Trp Ile Asp Leu Arg Ser Leu Cys 
        755                 760                 765             


Leu Phe Ser Tyr His His Leu Arg Asp Leu Leu Leu Ile Val Thr Arg 
    770                 775                 780                 


Ile Val Glu Ile Leu Gly Arg Arg Gly Trp Glu Ile Leu Lys Tyr Trp 
785                 790                 795                 800 


Trp Asn Leu Leu Gln Tyr Trp Ile Gln Glu Leu Lys Asn Ser Ala Val 
                805                 810                 815     


Ser Leu Leu Asn Ala Ile Ala Ile Ala Val Gly Glu Gly Thr Asp Arg 
            820                 825                 830         


Ile Ile Glu Ala Phe Arg Ser Ile Phe Arg Ala Ile Leu His Ile Pro 
        835                 840                 845             


Thr Arg Ile Arg Gln Gly Leu Glu Arg Ser Leu Leu 
    850                 855                 860 


<210>  3
<211>  2583
<212>  DNA
<213>  Artificial

<220>
<223>  polynucleotide encoding a fragment of env polypeptide of the 
       LR1-C1 isolate

<400>  3
atgagagtga gggagaccag gaagaattat cagcacttgt ggtggaaatg gggcatgatg       60

ctccttggga tgttgatgat ctgtagtgct gtagaacaaa cgtgggtcac agtctattat      120

ggggtacctg tgtggaaaga agcaaacacc attttatttt gtgcatcaga tgctaaagca      180

tataatacag aggtacataa tgtttgggcc acacatgcct gtgtacccac agaccccaac      240

ccacaagaag tagaattgga agatgtgaca gaaaatttta acatgtggaa aaacaacatg      300

gtagatcaga tgcatgggga tataatcagt ttatgggatc aaagcctaaa gccatgtgta      360

aaattaactc cactctgtgt tactttaagt tacaatgata atgtgggaaa tggtactagt      420

accaataata gtagatggga taaaatggaa aaaggagaaa taaagaactg ctctttctat      480

atcaccacaa acatgagaga taagatgcag aaacaatatg cactttttta taaacttgat      540

gtagtaccaa tagaggaagg taagaataat gacagtagtt ttaccgacta taggttgata      600

agttgtaaca cctcagtcat tacacaggcc tgtccaaagg taacctttga gccaattccc      660

atacattatt gtgtcccagc tggttttgcg cttctaaaat gtaaggataa gaaattcaat      720

ggaacaggac catgtaaaaa tgttagcaca gtacaatgta cacatggaat taagccagta      780

gtatcaactc agctgctatt aaatggcagt ctagcagaag aagaggtagt aataagatct      840

gagaacttct cgaacaatgc tagaaccata atagtacagc tgaatacatc tgtagaaata      900

aagtgtataa gacccaacaa caatacaaga aaaggtatac atataggacc agggagagca      960

ttttatacaa caggagatat aataggagat ataagacaag cacattgtaa cattagtaga     1020

caaaattgga ataacacttt aaaacagata gctgaaaagt taagagaaca atttgggaat     1080

aaaacaatag tctttagaaa ctcctctgga ggggatccag aaattgtaat gcacactttt     1140

aattgtgcag gggaattttt ctactgtaat acagcagaac tgtttaatag tacttggtat     1200

gcaaatggca caattagtat tggaggggga aacaagacta atatcatact cccatgcaga     1260

ataaaacaat ttataaacat gtggcaagaa gtaggaaaag caatgtatgc ccctcccatc     1320

agtggacaga ttagatgttc atcaaatatt acaggactgc tattaacaag agatggtggt     1380

aggggcaatc agaccgacaa ccagactgag atcttcagac ctgtaggagg agatataaaa     1440

aacaattgga gaagtgaatt atataaatat aaagtagtaa gaattgaacc attaggaata     1500

gcacccacca gggcaaaatg gagagtggtg cagagagaaa aaagagcagt gggaatagga     1560

gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     1620

ctgacggtac aggccagact attattgtct ggtatagtgc aacagcagaa caatctgctg     1680

agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     1740

caggcaagag tcctggctgt ggaaagatac ctacgtgatc aacagctcct gggaatttgg     1800

ggttgctctg gaaaactcat ctgcaccact gctgtgcctt ggaatgttag ttggaataat     1860

agatctgtgg atgacatttg ggaaaacatg acctggatgc agtgggatag agaaattagt     1920

aattacacaa gtttaataaa caccttaatt gaagaatcgc agaatcagca agaaaagaat     1980

gaacaagaat tattggcatt agataaatgg gcaaatttgt ggaattggtt caacataaca     2040

gaatggctgt ggtatataaa aatattcata atgatagtag gaggcttggt aggtttaaga     2100

atagtttttg ctgtgctttc tatagtgaat agagttaggc agggatactc accattatcg     2160

tttcagaccc acctcccagc tcagagggga cccgacaggc ccggaggaat cgaagaagaa     2220

ggtggagaga gcgacagaga cagatccgga agattagtga atggattctt agcaattatc     2280

tggatcgacc tgcggagcct gtgccttttc agctaccacc acttgagaga cttactattg     2340

attgtaacga ggattgtgga aattctggga cgcagggggt gggaaatcct caagtattgg     2400

tggaatctcc tgcagtattg gattcaggaa ctaaagaata gtgctgttag cttgctcaac     2460

gccatagcca tagcagtagg tgaggggaca gataggatta tagaagcatt tagaagcatt     2520

tttagagcta ttctccacat acctacaaga ataagacagg gcttggaaag gtctttgcta     2580

taa                                                                   2583


<210>  4
<211>  860
<212>  PRT
<213>  Artificial

<220>
<223>  env polypeptide of the LR1-C1 isolate

<400>  4

Met Arg Val Arg Glu Thr Arg Lys Asn Tyr Gln His Leu Trp Trp Lys 
1               5                   10                  15      


Trp Gly Met Met Leu Leu Gly Met Leu Met Ile Cys Ser Ala Val Glu 
            20                  25                  30          


Gln Thr Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
        35                  40                  45              


Asn Thr Ile Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asn Thr Glu 
    50                  55                  60                  


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
65                  70                  75                  80  


Pro Gln Glu Val Glu Leu Glu Asp Val Thr Glu Asn Phe Asn Met Trp 
                85                  90                  95      


Lys Asn Asn Met Val Asp Gln Met His Gly Asp Ile Ile Ser Leu Trp 
            100                 105                 110         


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
        115                 120                 125             


Leu Ser Tyr Asn Asp Asn Val Gly Asn Gly Thr Ser Thr Asn Asn Ser 
    130                 135                 140                 


Arg Trp Asp Lys Met Glu Lys Gly Glu Ile Lys Asn Cys Ser Phe Tyr 
145                 150                 155                 160 


Ile Thr Thr Asn Met Arg Asp Lys Met Gln Lys Gln Tyr Ala Leu Phe 
                165                 170                 175     


Tyr Lys Leu Asp Val Val Pro Ile Glu Glu Gly Lys Asn Asn Asp Ser 
            180                 185                 190         


Ser Phe Thr Asp Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr 
        195                 200                 205             


Gln Ala Cys Pro Lys Val Thr Phe Glu Pro Ile Pro Ile His Tyr Cys 
    210                 215                 220                 


Val Pro Ala Gly Phe Ala Leu Leu Lys Cys Lys Asp Lys Lys Phe Asn 
225                 230                 235                 240 


Gly Thr Gly Pro Cys Lys Asn Val Ser Thr Val Gln Cys Thr His Gly 
                245                 250                 255     


Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala 
            260                 265                 270         


Glu Glu Glu Val Val Ile Arg Ser Glu Asn Phe Ser Asn Asn Ala Arg 
        275                 280                 285             


Thr Ile Ile Val Gln Leu Asn Thr Ser Val Glu Ile Lys Cys Ile Arg 
    290                 295                 300                 


Pro Asn Asn Asn Thr Arg Lys Gly Ile His Ile Gly Pro Gly Arg Ala 
305                 310                 315                 320 


Phe Tyr Thr Thr Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys 
                325                 330                 335     


Asn Ile Ser Arg Gln Asn Trp Asn Asn Thr Leu Lys Gln Ile Ala Glu 
            340                 345                 350         


Lys Leu Arg Glu Gln Phe Gly Asn Lys Thr Ile Val Phe Arg Asn Ser 
        355                 360                 365             


Ser Gly Gly Asp Pro Glu Ile Val Met His Thr Phe Asn Cys Ala Gly 
    370                 375                 380                 


Glu Phe Phe Tyr Cys Asn Thr Ala Glu Leu Phe Asn Ser Thr Trp Tyr 
385                 390                 395                 400 


Ala Asn Gly Thr Ile Ser Ile Gly Gly Gly Asn Lys Thr Asn Ile Ile 
                405                 410                 415     


Leu Pro Cys Arg Ile Lys Gln Phe Ile Asn Met Trp Gln Glu Val Gly 
            420                 425                 430         


Lys Ala Met Tyr Ala Pro Pro Ile Ser Gly Gln Ile Arg Cys Ser Ser 
        435                 440                 445             


Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Arg Gly Asn Gln 
    450                 455                 460                 


Thr Asp Asn Gln Thr Glu Ile Phe Arg Pro Val Gly Gly Asp Ile Lys 
465                 470                 475                 480 


Asn Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Arg Ile Glu 
                485                 490                 495     


Pro Leu Gly Ile Ala Pro Thr Arg Ala Lys Trp Arg Val Val Gln Arg 
            500                 505                 510         


Glu Lys Arg Ala Val Gly Ile Gly Ala Leu Phe Leu Gly Phe Leu Gly 
        515                 520                 525             


Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Gln 
    530                 535                 540                 


Ala Arg Leu Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu 
545                 550                 555                 560 


Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly 
                565                 570                 575     


Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Arg 
            580                 585                 590         


Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys 
        595                 600                 605             


Thr Thr Ala Val Pro Trp Asn Val Ser Trp Asn Asn Arg Ser Val Asp 
    610                 615                 620                 


Asp Ile Trp Glu Asn Met Thr Trp Met Gln Trp Asp Arg Glu Ile Ser 
625                 630                 635                 640 


Asn Tyr Thr Ser Leu Ile Asn Thr Leu Ile Glu Glu Ser Gln Asn Gln 
                645                 650                 655     


Gln Glu Lys Asn Glu Gln Glu Leu Leu Ala Leu Asp Lys Trp Ala Asn 
            660                 665                 670         


Leu Trp Asn Trp Phe Asn Ile Thr Glu Trp Leu Trp Tyr Ile Lys Ile 
        675                 680                 685             


Phe Ile Met Ile Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala 
    690                 695                 700                 


Val Leu Ser Ile Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser 
705                 710                 715                 720 


Phe Gln Thr His Leu Pro Ala Gln Arg Gly Pro Asp Arg Pro Gly Gly 
                725                 730                 735     


Ile Glu Glu Glu Gly Gly Glu Ser Asp Arg Asp Arg Ser Gly Arg Leu 
            740                 745                 750         


Val Asn Gly Phe Leu Ala Ile Ile Trp Ile Asp Leu Arg Ser Leu Cys 
        755                 760                 765             


Leu Phe Ser Tyr His His Leu Arg Asp Leu Leu Leu Ile Val Thr Arg 
    770                 775                 780                 


Ile Val Glu Ile Leu Gly Arg Arg Gly Trp Glu Ile Leu Lys Tyr Trp 
785                 790                 795                 800 


Trp Asn Leu Leu Gln Tyr Trp Ile Gln Glu Leu Lys Asn Ser Ala Val 
                805                 810                 815     


Ser Leu Leu Asn Ala Ile Ala Ile Ala Val Gly Glu Gly Thr Asp Arg 
            820                 825                 830         


Ile Ile Glu Ala Phe Arg Ser Ile Phe Arg Ala Ile Leu His Ile Pro 
        835                 840                 845             


Thr Arg Ile Arg Gln Gly Leu Glu Arg Ser Leu Leu 
    850                 855                 860 


<210>  5
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  primer aligning with a fragment of the HIV-1 HXB2 genome

<400>  5
tagagccctg gaagcatcca ggaag                                             25


<210>  6
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  primer aligning with a fragment of the HIV-1 HXB2 genome

<400>  6
ttgctacttg tgattgctcc atgt                                              24


<210>  7
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  primer aligning with a fragment of the HIV-1 HXB2 genome

<400>  7
agctggatcc gtctcgagat actgctccca ccc                                    33


<210>  8
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer aligning with a fragment of the HIV-1 HXB2 genome

<400>  8
gtagtacatg taatgcaacc                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer aligning with a fragment of the HIV-1 HXB2 genome

<400>  9
agctcgtctc attctttccc                                                   20


<210>  10
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  primer aligning with a fragment of the HIV-1 HXB2 genome

<400>  10
ccaattccca tacattattg tgc                                               23


<210>  11
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  primer aligning with a fragment of the HIV-1 HXB2 genome

<400>  11
ggagcagcag gaagcactat gggc                                              24


<210>  12
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  primer aligning with a fragment of the HIV-1 HXB2 genome

<400>  12
gagttaggca gggatactca cc                                                22


<210>  13
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  primer aligning with a fragment of the HIV-1 HXB2 genome

<400>  13
gccaggactc ttgcctggag ctg                                               23


<210>  14
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  primer aligning with a fragment of the HIV-1 HXB2 genome

<400>  14
cttgtattgt tgttgggtc                                                    19


<210>  15
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  primer aligning with a fragment of the HIV-1 HXB2 genome

<400>  15
catggcttta ggctttgatc cc                                                22


<210>  16
<211>  103
<212>  PRT
<213>  Artificial

<220>
<223>  Fragment of gp160 preprotein encoded by the env gene of the HIV 
       AC10 wild-type isolate

<400>  16

Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu 
1               5                   10                  15      


Ser Cys Thr Asp Asn Val Gly Asn Asp Thr Ser Thr Asn Asn Ser Arg 
            20                  25                  30          


Trp Asp Lys Met Glu Lys Gly Glu Ile Lys Asn Cys Ser Phe Asn Ile 
        35                  40                  45              


Thr Thr Asn Met Arg Asp Lys Met Gln Lys Gln Tyr Ala Leu Phe Tyr 
    50                  55                  60                  


Lys Leu Asp Val Val Pro Ile Glu Glu Gly Lys Asn Asn Asn Ser Ser 
65                  70                  75                  80  


Phe Thr Asp Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr Gln 
                85                  90                  95      


Ala Cys Pro Lys Val Thr Phe 
            100             


<210>  17
<211>  103
<212>  PRT
<213>  Artificial

<220>
<223>  fragment of env polypeptide of the LR1-C1 isolate

<400>  17

Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu 
1               5                   10                  15      


Ser Tyr Asn Asp Asn Val Gly Asn Gly Thr Ser Thr Asn Asn Ser Arg 
            20                  25                  30          


Trp Asp Lys Met Glu Lys Gly Glu Ile Lys Asn Cys Ser Phe Tyr Ile 
        35                  40                  45              


Thr Thr Asn Met Arg Asp Lys Met Gln Lys Gln Tyr Ala Leu Phe Tyr 
    50                  55                  60                  


Lys Leu Asp Val Val Pro Ile Glu Glu Gly Lys Asn Asn Asp Ser Ser 
65                  70                  75                  80  


Phe Thr Asp Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr Gln 
                85                  90                  95      


Ala Cys Pro Lys Val Thr Phe 
            100             


<210>  18
<211>  40
<212>  PRT
<213>  Artificial

<220>
<223>  ENV AC10 sequenced fragment

<400>  18

Lys Leu Thr Pro Leu Cys Val Thr Leu Ser Cys Thr Asp Asn Val Gly 
1               5                   10                  15      


Asn Asp Thr Ser Thr Asn Asn Ser Arg Trp Asp Lys Met Glu Lys Gly 
            20                  25                  30          


Glu Ile Lys Asn Cys Ser Phe Asn 
        35                  40  


<210>  19
<211>  40
<212>  PRT
<213>  Artificial

<220>
<223>  L1-1 contig ENV

<400>  19

Lys Leu Thr Pro Leu Cys Val Thr Leu Ser Cys Thr Asp Asn Val Gly 
1               5                   10                  15      


Asn Asp Thr Ser Thr Asn Asn Ser Arg Trp Asp Lys Met Glu Lys Gly 
            20                  25                  30          


Glu Ile Lys Asn Cys Tyr Phe Asn 
        35                  40  


<210>  20
<211>  40
<212>  PRT
<213>  Artificial

<220>
<223>  L1-2 contig ENV

<400>  20

Lys Leu Thr Gln Leu Cys Asp Thr Leu Ser Cys Thr Asp Asn Val Gly 
1               5                   10                  15      


Asn Asp Thr Ser Thr Asn Asn Ser Arg Trp Asp Lys Met Glu Lys Gly 
            20                  25                  30          


Glu Ile Lys Asn Cys Ser Phe Asn 
        35                  40  


<210>  21
<211>  40
<212>  PRT
<213>  Artificial

<220>
<223>  L1-3 contig ENV

<400>  21

Lys Leu Thr Pro Leu Cys Val Thr Leu Ser Cys Thr Asp Asn Glu Gly 
1               5                   10                  15      


Asn Asp Thr Ser Thr Asn Asn Ser Arg Trp Asp Lys Met Gly Lys Gly 
            20                  25                  30          


Glu Ile Lys Asn Cys Ser Phe Asn 
        35                  40  


<210>  22
<211>  40
<212>  PRT
<213>  Artificial

<220>
<223>  L1-6 contig ENV


<220>
<221>  VARIANT
<222>  (29)..(29)
<223>  /note=wherein Xaa is any amino acid

<400>  22

Lys Leu Thr Pro Leu Cys Val Thr Leu Ser Cys Thr Asp Asn Val Gly 
1               5                   10                  15      


Asn Asp Thr Ser Thr Asn Asn Ser Arg Trp Asp Lys Xaa Glu Lys Gly 
            20                  25                  30          


Glu Ile Lys Asn Cys Ser Phe Asn 
        35                  40  


<210>  23
<211>  40
<212>  PRT
<213>  Artificial

<220>
<223>  L2-2 contig ENV


<220>
<221>  VARIANT
<222>  (35)..(35)
<223>  /note= wherein Xaa is any amino acid

<400>  23

Lys Leu Thr Pro Leu Cys Val Thr Leu Ser Cys Thr Asp Asn Val Gly 
1               5                   10                  15      


Asn Asp Thr Ser Thr Asn Asn Ser Arg Trp Asp Lys Met Glu Lys Gly 
            20                  25                  30          


Glu Ile Xaa Asn Cys Ser Phe Asn 
        35                  40  


<210>  24
<211>  40
<212>  PRT
<213>  Artificial

<220>
<223>  L2-3 contig ENV

<400>  24

Lys Leu Thr Pro Leu Cys Val Thr Leu Ser Cys Thr Asp Asn Val Gly 
1               5                   10                  15      


Asn Asp Ile Ser Thr Ile Asn Ser Arg Trp Asp Lys Met Glu Lys Gly 
            20                  25                  30          


Glu Ile Lys Asn Cys Ser Cys Asn 
        35                  40  


<210>  25
<211>  40
<212>  PRT
<213>  Artificial

<220>
<223>  L3-4 contig ENV

<400>  25

Lys Leu Thr Pro Leu Cys Val Thr Leu Ser Cys Thr Asp Asn Met Gly 
1               5                   10                  15      


Asn Asp Thr Ser Thr Asn Asn Ser Arg Trp Asp Lys Met Glu Lys Gly 
            20                  25                  30          


Glu Ile Lys Asn Cys Ser Phe Asn 
        35                  40  


<210>  26
<211>  40
<212>  PRT
<213>  Artificial

<220>
<223>  Contig L2 clon 5 pNL43

<400>  26

Lys Leu Thr Pro Leu Cys Val Thr Leu Ser Cys Thr Asp Asn Val Gly 
1               5                   10                  15      


Asn Asp Thr Ser Thr Asn Asn Ser Arg Trp Asp Lys Met Glu Lys Gly 
            20                  25                  30          


Glu Ile Lys Asn Phe Ser Phe Asn 
        35                  40  


<210>  27
<211>  40
<212>  PRT
<213>  Artificial

<220>
<223>  Contig BR1 clon 1 pcDNA

<400>  27

Lys Leu Thr Pro Leu Cys Val Thr Leu Ser Tyr Asn Asp Asn Val Gly 
1               5                   10                  15      


Asn Asp Thr Ser Thr Asn Asn Ser Arg Trp Asp Lys Met Glu Lys Gly 
            20                  25                  30          


Glu Ile Lys Asn Cys Ser Phe Asn 
        35                  40  


<210>  28
<211>  40
<212>  PRT
<213>  Artificial

<220>
<223>  Contig BR1 clon 3 pcDNA

<400>  28

Lys Leu Thr Pro Leu Cys Val Thr Leu Ser Tyr Lys Asp Asn Val Gly 
1               5                   10                  15      


Asn Asp Thr Ser Thr Asn Asn Ser Arg Trp Asp Lys Met Glu Lys Gly 
            20                  25                  30          


Glu Ile Lys Asn Cys Ser Phe Asn 
        35                  40  


<210>  29
<211>  40
<212>  PRT
<213>  Artificial

<220>
<223>  Contig BR1 clon 1

<400>  29

Lys Leu Thr Pro Leu Cys Val Thr Leu Ser Tyr Asn Asp Asn Val Gly 
1               5                   10                  15      


Asn Asp Gly Ser Thr Asn Asn Ser Arg Trp Asp Lys Met Glu Lys Gly 
            20                  25                  30          


Glu Ile Lys Asn Cys Ser Phe Tyr 
        35                  40  


<210>  30
<211>  2583
<212>  DNA
<213>  Artificial

<220>
<223>  Env nucleotide sequence selected with PG16-C10 antibody

<400>  30
atgagagtga gggagaccag gaagaattat cagcacttgt ggtggaaatg gggcatgatg       60

ctccttggga tgttgatgat ctgtagtgct gtagaacaaa cgtgggtcac agtctattat      120

ggggtacctg tgtggaaaga agcaaacacc attttatttt gtgcatcaga tgctaaagca      180

tataatacag aggtacataa tgtttgggcc acacatgcct gtgtacccac agaccccaac      240

ccacaagaag tagaattgga aaatgtgaca gaaaatttta acatgtggaa aaacaacatg      300

gtagatcaga tgcatgggga tataatcagt ttatgggatc aaagcctaaa gccatgtgta      360

aaattaactc cactctgtgt tactttaagt tgcactgata atgtgggaaa tgatactagt      420

accaataata gtagatggga taaaatggaa aaaggagaaa taaagaactg ctctttcaat      480

atcaccacaa acatgagaga taagatgcag aaacaatatg cactttttta taaacttgat      540

gtagtaccaa tagaggaagg taagaataat aacagtagtt ttaccgacta taggttgata      600

agttgtagca cctcagtcat tacacaggcc tgtccaaagg taacctttga gccaattccc      660

atacattatt gtgccccagc tggttttgcg cttctaaaat gtaaggataa gaaattcaat      720

ggaaccggac catgtaaaaa tgttagcaca gtacaatgta cacatggaat taagccagta      780

gtatcaactc agctgctatt aaatggcagt ctagcagaag aagaggtagt aataagatct      840

gagaacttct cgaacaatgc tagaaccata atagtacagc tgaatacatc tgtagaaata      900

aagtgtataa gacccaacaa caatacaaga aaaggtatac atataggacc agggagagca      960

ttttatacaa caggagatat aataggagat ataagacaag cacattgtaa cattagtaga     1020

caaaattgga ataacacttt aaaacagata gctgaaaagt taagagaaca atttgggaat     1080

aaaacaatag tctttagaaa ctcctctgga ggggatccag aaattgtaat gcacactttt     1140

aattgtgcag gggaattttt ctactgtaat acagcagaac tgtttaatag tacttggtat     1200

gcaaatggca caattagtat tggaggggga aacaagacta atatcatact cccatgcaga     1260

ataaaacaat ttataaacat gtggcaggaa gtaggaaaag caatgtatgc ccctcccatc     1320

agtggacaga ttagatgttc atcaaatatt acaggactgc tattaacaag agatggtggt     1380

aggggcaatc agaccgacaa ccagactgag atcttcagac ctgtaggagg agatatgaaa     1440

aacaattgga gaagtgaatt atataaatat aaagtagtaa gaattgaacc attaggaata     1500

gcacccacca gggcaaaaag gagagtggtg cagagagaaa aaagagcagt gggaatagga     1560

gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg     1620

ctgacggtac aggccagact attattgtct ggtatagtgc aacagcagaa caatctgctg     1680

agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc     1740

caggcaagag tcctggctgt ggaaagatac ctacgtgatc aacagctcct gggaatttgg     1800

ggttgctctg aaaaactcat ctgcaccact gctgtgcctt ggaatgttag ttggaataat     1860

agatctgtgg atgacatttg ggaaaacatg acctggatgc agtgggatag agaaattagt     1920

aattacacaa gtttaatata caccttaatt gaagaatcgc agaatcagca agaaaagaat     1980

gaacaagaat tattggcatt agataaatgg gcaaatttgt ggaattggtt caacataaca     2040

gaatggctgt ggtatataaa aatattcata atgatagtag gaggcttggt aggtttaaga     2100

atagtttttg ctgtgctttc tatagtgaat agagttaggc agggatactc accattatcg     2160

tttcagaccc acctcccagc tcagagggga cccgacaggc ccggaggaat cgaagaagaa     2220

ggtggagaga gcgacagaga cagatccgga agattagtga atggattctt agcaattatc     2280

tggatcgacc tgcggagcct gtgccttttc agctaccacc acttgagaga cttactattg     2340

attgtaacga ggattgtgga aattctggga cgcagggggt gggaaatcct caagtattgg     2400

tggaatctcc tgcagtattg gattcaggaa ctaaagaata gtgctgttag cctgctcaac     2460

gccatagcca tagcagtagg tgaggggaca gataggatta tagaagcatt tagaagcatt     2520

tttagagcta ttctccacat acctacaaga ataagacagg gcttggaaag gtctttgcta     2580

taa                                                                   2583


<210>  31
<211>  860
<212>  PRT
<213>  Artificial

<220>
<223>  PG16 clone 10

<400>  31

Met Arg Val Arg Glu Thr Arg Lys Asn Tyr Gln His Leu Trp Trp Lys 
1               5                   10                  15      


Trp Gly Met Met Leu Leu Gly Met Leu Met Ile Cys Ser Ala Val Glu 
            20                  25                  30          


Gln Thr Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
        35                  40                  45              


Asn Thr Ile Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asn Thr Glu 
    50                  55                  60                  


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
65                  70                  75                  80  


Pro Gln Glu Val Glu Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp 
                85                  90                  95      


Lys Asn Asn Met Val Asp Gln Met His Gly Asp Ile Ile Ser Leu Trp 
            100                 105                 110         


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
        115                 120                 125             


Leu Ser Cys Thr Asp Asn Val Gly Asn Asp Thr Ser Thr Asn Asn Ser 
    130                 135                 140                 


Arg Trp Asp Lys Met Glu Lys Gly Glu Ile Lys Asn Cys Ser Phe Asn 
145                 150                 155                 160 


Ile Thr Thr Asn Met Arg Asp Lys Met Gln Lys Gln Tyr Ala Leu Phe 
                165                 170                 175     


Tyr Lys Leu Asp Val Val Pro Ile Glu Glu Gly Lys Asn Asn Asn Ser 
            180                 185                 190         


Ser Phe Thr Asp Tyr Arg Leu Ile Ser Cys Ser Thr Ser Val Ile Thr 
        195                 200                 205             


Gln Ala Cys Pro Lys Val Thr Phe Glu Pro Ile Pro Ile His Tyr Cys 
    210                 215                 220                 


Ala Pro Ala Gly Phe Ala Leu Leu Lys Cys Lys Asp Lys Lys Phe Asn 
225                 230                 235                 240 


Gly Thr Gly Pro Cys Lys Asn Val Ser Thr Val Gln Cys Thr His Gly 
                245                 250                 255     


Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala 
            260                 265                 270         


Glu Glu Glu Val Val Ile Arg Ser Glu Asn Phe Ser Asn Asn Ala Arg 
        275                 280                 285             


Thr Ile Ile Val Gln Leu Asn Thr Ser Val Glu Ile Lys Cys Ile Arg 
    290                 295                 300                 


Pro Asn Asn Asn Thr Arg Lys Gly Ile His Ile Gly Pro Gly Arg Ala 
305                 310                 315                 320 


Phe Tyr Thr Thr Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys 
                325                 330                 335     


Asn Ile Ser Arg Gln Asn Trp Asn Asn Thr Leu Lys Gln Ile Ala Glu 
            340                 345                 350         


Lys Leu Arg Glu Gln Phe Gly Asn Lys Thr Ile Val Phe Arg Asn Ser 
        355                 360                 365             


Ser Gly Gly Asp Pro Glu Ile Val Met His Thr Phe Asn Cys Ala Gly 
    370                 375                 380                 


Glu Phe Phe Tyr Cys Asn Thr Ala Glu Leu Phe Asn Ser Thr Trp Tyr 
385                 390                 395                 400 


Ala Asn Gly Thr Ile Ser Ile Gly Gly Gly Asn Lys Thr Asn Ile Ile 
                405                 410                 415     


Leu Pro Cys Arg Ile Lys Gln Phe Ile Asn Met Trp Gln Glu Val Gly 
            420                 425                 430         


Lys Ala Met Tyr Ala Pro Pro Ile Ser Gly Gln Ile Arg Cys Ser Ser 
        435                 440                 445             


Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Arg Gly Asn Gln 
    450                 455                 460                 


Thr Asp Asn Gln Thr Glu Ile Phe Arg Pro Val Gly Gly Asp Met Lys 
465                 470                 475                 480 


Asn Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Arg Ile Glu 
                485                 490                 495     


Pro Leu Gly Ile Ala Pro Thr Arg Ala Lys Arg Arg Val Val Gln Arg 
            500                 505                 510         


Glu Lys Arg Ala Val Gly Ile Gly Ala Leu Phe Leu Gly Phe Leu Gly 
        515                 520                 525             


Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Gln 
    530                 535                 540                 


Ala Arg Leu Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu 
545                 550                 555                 560 


Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly 
                565                 570                 575     


Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Arg 
            580                 585                 590         


Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Glu Lys Leu Ile Cys 
        595                 600                 605             


Thr Thr Ala Val Pro Trp Asn Val Ser Trp Asn Asn Arg Ser Val Asp 
    610                 615                 620                 


Asp Ile Trp Glu Asn Met Thr Trp Met Gln Trp Asp Arg Glu Ile Ser 
625                 630                 635                 640 


Asn Tyr Thr Ser Leu Ile Tyr Thr Leu Ile Glu Glu Ser Gln Asn Gln 
                645                 650                 655     


Gln Glu Lys Asn Glu Gln Glu Leu Leu Ala Leu Asp Lys Trp Ala Asn 
            660                 665                 670         


Leu Trp Asn Trp Phe Asn Ile Thr Glu Trp Leu Trp Tyr Ile Lys Ile 
        675                 680                 685             


Phe Ile Met Ile Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala 
    690                 695                 700                 


Val Leu Ser Ile Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser 
705                 710                 715                 720 


Phe Gln Thr His Leu Pro Ala Gln Arg Gly Pro Asp Arg Pro Gly Gly 
                725                 730                 735     


Ile Glu Glu Glu Gly Gly Glu Ser Asp Arg Asp Arg Ser Gly Arg Leu 
            740                 745                 750         


Val Asn Gly Phe Leu Ala Ile Ile Trp Ile Asp Leu Arg Ser Leu Cys 
        755                 760                 765             


Leu Phe Ser Tyr His His Leu Arg Asp Leu Leu Leu Ile Val Thr Arg 
    770                 775                 780                 


Ile Val Glu Ile Leu Gly Arg Arg Gly Trp Glu Ile Leu Lys Tyr Trp 
785                 790                 795                 800 


Trp Asn Leu Leu Gln Tyr Trp Ile Gln Glu Leu Lys Asn Ser Ala Val 
                805                 810                 815     


Ser Leu Leu Asn Ala Ile Ala Ile Ala Val Gly Glu Gly Thr Asp Arg 
            820                 825                 830         


Ile Ile Glu Ala Phe Arg Ser Ile Phe Arg Ala Ile Leu His Ile Pro 
        835                 840                 845             


Thr Arg Ile Arg Gln Gly Leu Glu Arg Ser Leu Leu 
    850                 855                 860 


<210>  32
<211>  860
<212>  PRT
<213>  Artificial

<220>
<223>  Sequence Env AC10

<400>  32

Met Arg Val Arg Glu Thr Arg Lys Asn Tyr Gln His Leu Trp Trp Lys 
1               5                   10                  15      


Trp Gly Met Met Leu Leu Gly Met Leu Met Ile Cys Ser Ala Val Glu 
            20                  25                  30          


Gln Thr Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
        35                  40                  45              


Asn Thr Ile Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asn Thr Glu 
    50                  55                  60                  


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
65                  70                  75                  80  


Pro Gln Glu Val Glu Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp 
                85                  90                  95      


Lys Asn Asn Met Val Asp Gln Met His Gly Asp Ile Ile Ser Leu Trp 
            100                 105                 110         


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
        115                 120                 125             


Leu Ser Cys Thr Asp Asn Val Gly Asn Asp Thr Ser Thr Asn Asn Ser 
    130                 135                 140                 


Arg Trp Asp Lys Met Glu Lys Gly Glu Ile Lys Asn Cys Ser Phe Asn 
145                 150                 155                 160 


Ile Thr Thr Asn Met Arg Asp Lys Met Gln Lys Gln Tyr Ala Leu Phe 
                165                 170                 175     


Tyr Lys Leu Asp Val Val Pro Ile Glu Glu Gly Lys Asn Asn Asn Ser 
            180                 185                 190         


Ser Phe Thr Asp Tyr Arg Leu Ile Ser Cys Asn Thr Ser Val Ile Thr 
        195                 200                 205             


Gln Ala Cys Pro Lys Val Thr Phe Glu Pro Ile Pro Ile His Tyr Cys 
    210                 215                 220                 


Ala Pro Ala Gly Phe Ala Leu Leu Lys Cys Lys Asp Lys Lys Phe Asn 
225                 230                 235                 240 


Gly Thr Gly Pro Cys Lys Asn Val Ser Thr Val Gln Cys Thr His Gly 
                245                 250                 255     


Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala 
            260                 265                 270         


Glu Glu Glu Val Val Ile Arg Ser Glu Asn Phe Ser Asn Asn Ala Arg 
        275                 280                 285             


Thr Ile Ile Val Gln Leu Asn Thr Ser Val Glu Ile Lys Cys Ile Arg 
    290                 295                 300                 


Pro Asn Asn Asn Thr Arg Lys Gly Ile His Ile Gly Pro Gly Arg Ala 
305                 310                 315                 320 


Phe Tyr Thr Thr Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys 
                325                 330                 335     


Asn Ile Ser Arg Gln Asn Trp Asn Asn Thr Leu Lys Gln Ile Ala Glu 
            340                 345                 350         


Lys Leu Arg Glu Gln Phe Gly Asn Lys Thr Ile Val Phe Arg Asn Ser 
        355                 360                 365             


Ser Gly Gly Asp Pro Glu Ile Val Met His Thr Phe Asn Cys Ala Gly 
    370                 375                 380                 


Glu Phe Phe Tyr Cys Asn Thr Ala Glu Leu Phe Asn Ser Thr Trp Tyr 
385                 390                 395                 400 


Ala Asn Gly Thr Ile Ser Ile Gly Gly Gly Asn Lys Thr Asn Ile Ile 
                405                 410                 415     


Leu Pro Cys Arg Ile Lys Gln Phe Ile Asn Met Trp Gln Glu Val Gly 
            420                 425                 430         


Lys Ala Met Tyr Ala Pro Pro Ile Ser Gly Gln Ile Arg Cys Ser Ser 
        435                 440                 445             


Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Arg Gly Asn Gln 
    450                 455                 460                 


Thr Asp Asn Gln Thr Glu Ile Phe Arg Pro Val Gly Gly Asp Met Lys 
465                 470                 475                 480 


Asn Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Arg Ile Glu 
                485                 490                 495     


Pro Leu Gly Ile Ala Pro Thr Arg Ala Lys Arg Arg Val Val Gln Arg 
            500                 505                 510         


Glu Lys Arg Ala Val Gly Ile Gly Ala Leu Phe Leu Gly Phe Leu Gly 
        515                 520                 525             


Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Gln 
    530                 535                 540                 


Ala Arg Leu Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu 
545                 550                 555                 560 


Arg Ala Ile Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly 
                565                 570                 575     


Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Arg 
            580                 585                 590         


Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys 
        595                 600                 605             


Thr Thr Ala Val Pro Trp Asn Val Ser Trp Asn Asn Arg Ser Val Asp 
    610                 615                 620                 


Asp Ile Trp Glu Asn Met Thr Trp Met Gln Trp Asp Arg Glu Ile Ser 
625                 630                 635                 640 


Asn Tyr Thr Ser Leu Ile Tyr Thr Leu Ile Glu Glu Ser Gln Asn Gln 
                645                 650                 655     


Gln Glu Lys Asn Glu Gln Glu Leu Leu Ala Leu Asp Lys Trp Ala Asn 
            660                 665                 670         


Leu Trp Asn Trp Phe Asn Ile Thr Glu Trp Leu Trp Tyr Ile Lys Ile 
        675                 680                 685             


Phe Ile Met Ile Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala 
    690                 695                 700                 


Val Leu Ser Ile Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser 
705                 710                 715                 720 


Phe Gln Thr His Leu Pro Ala Gln Arg Gly Pro Asp Arg Pro Gly Gly 
                725                 730                 735     


Ile Glu Glu Glu Gly Gly Glu Ser Asp Arg Asp Arg Ser Gly Arg Leu 
            740                 745                 750         


Val Asn Gly Phe Leu Ala Ile Ile Trp Ile Asp Leu Arg Ser Leu Cys 
        755                 760                 765             


Leu Phe Ser Tyr His His Leu Arg Asp Leu Leu Leu Ile Val Thr Arg 
    770                 775                 780                 


Ile Val Glu Ile Leu Gly Arg Arg Gly Trp Glu Ile Leu Lys Tyr Trp 
785                 790                 795                 800 


Trp Asn Leu Leu Gln Tyr Trp Ile Gln Glu Leu Lys Asn Ser Ala Val 
                805                 810                 815     


Ser Leu Leu Asn Ala Ile Ala Ile Ala Val Gly Glu Gly Thr Asp Arg 
            820                 825                 830         


Ile Ile Glu Ala Phe Arg Ser Ile Phe Arg Ala Ile Leu His Ile Pro 
        835                 840                 845             


Thr Arg Ile Arg Gln Gly Leu Glu Arg Ser Leu Leu 
    850                 855                 860 


