                         SEQUENCE LISTING

<110>  Bristol-Myers Squibb Company
       Goswami, Animesh
       Goldberg, Steven L.
       Johnston, Robert M.
       Hanson, Ronald L.
       Parker, William L.
 
<120>  REAGENTS USEFUL FOR THE PREPARATION OF AMINO ACIDS FROM KETO 
       ACIDS

<130>  11697WOPCT

<150>  61/439044
<151>  2011-02-03

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-terminal primer

<400>  1
gaccatatga gtggaattcg agtag                                             25


<210>  2
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C-terminal Primer

<400>  2
gcaggtacct tataatagtt ccttacg                                           27


<210>  3
<211>  990
<212>  DNA
<213>  Bacillus sphaericus

<400>  3
catatgagtg caattcgagt aggtattgta ggatatggaa atttaggacg tggtgtggag       60

tatgccattt cacaaaatcc agatatggaa ttagtagcag tgttcacacg acgtgatcct      120

tcaacagtaa acgtagcaag caatgcgagc gtatatttag tagatgacgc tgaaaaattc      180

caaaacgaca ttgatgttat gattttatgt ggtggttctg caacagattt accagagcaa      240

ggtccacatt ttgcacaatg gtttaataca attgatagct tcgatacaca tgcgaaaatt      300

cctgaatttt tcgatgctgt agatgcagcg gcacaaaaat ctggtaaagt atctgttatc      360

tcagttggtt gggatccagg tctattctct ttaaatcgtc tacttggaga atcagtatta      420

ccagttggta caacatatac attctggggc gatggcctaa gtcaaggaca ttcagatgct      480

gttcgtcgta ttgaaggcgt aaaaaatgct gttcaataca cattaccgat taaagaagcg      540

gtagaacgag ttcgtaacgg tgaaaaccct gaactaacaa cacgtgaaaa acatgcacgt      600

gaatgttggg ttgtattaga agaaggtgca gatgcaacaa aggttgaaca agaaattgtc      660

aaaatgccta actatttcga tgaatatgac acaacagtga acttcatttc tgaagatgaa      720

tttaaagcaa atcatactgg tatgccacat ggtggttttg tcattcgcag tggtgacagt      780

ggtgcaaacg ataaacaaat tctcgaattc tcattaaaac tggacagtaa tccaaacttt      840

acatctagtg tattagtggc ttatgcacgt gcagcacacc gtttaagtca agcgggtgat      900

aaaggtgcga aaacagtatt tgatatccca ttcagcctgc tttcgccaaa atcagctgct      960

gaattacgta aggaactatt ataaggatcc                                       990


<210>  4
<211>  326
<212>  PRT
<213>  Bacillus sphaericus

<400>  4

Met Ser Ala Ile Arg Val Gly Ile Val Gly Tyr Gly Asn Leu Gly Arg 
1               5                   10                  15      


Gly Val Glu Tyr Ala Ile Ser Gln Asn Pro Asp Met Glu Leu Val Ala 
            20                  25                  30          


Val Phe Thr Arg Arg Asp Pro Ser Thr Val Asn Val Ala Ser Asn Ala 
        35                  40                  45              


Ser Val Tyr Leu Val Asp Asp Ala Glu Lys Phe Gln Asn Asp Ile Asp 
    50                  55                  60                  


Val Met Ile Leu Cys Gly Gly Ser Ala Thr Asp Leu Pro Glu Gln Gly 
65                  70                  75                  80  


Pro His Phe Ala Gln Trp Phe Asn Thr Ile Asp Ser Phe Asp Thr His 
                85                  90                  95      


Ala Lys Ile Pro Glu Phe Phe Asp Ala Val Asp Ala Ala Ala Gln Lys 
            100                 105                 110         


Ser Gly Lys Val Ser Val Ile Ser Val Gly Trp Asp Pro Gly Leu Phe 
        115                 120                 125             


Ser Leu Asn Arg Leu Leu Gly Glu Ser Val Leu Pro Val Gly Thr Thr 
    130                 135                 140                 


Tyr Thr Phe Trp Gly Asp Gly Leu Ser Gln Gly His Ser Asp Ala Val 
145                 150                 155                 160 


Arg Arg Ile Glu Gly Val Lys Asn Ala Val Gln Tyr Thr Leu Pro Ile 
                165                 170                 175     


Lys Glu Ala Val Glu Arg Val Arg Asn Gly Glu Asn Pro Glu Leu Thr 
            180                 185                 190         


Thr Arg Glu Lys His Ala Arg Glu Cys Trp Val Val Leu Glu Glu Gly 
        195                 200                 205             


Ala Asp Ala Thr Lys Val Glu Gln Glu Ile Val Lys Met Pro Asn Tyr 
    210                 215                 220                 


Phe Asp Glu Tyr Asp Thr Thr Val Asn Phe Ile Ser Glu Asp Glu Phe 
225                 230                 235                 240 


Lys Ala Asn His Thr Gly Met Pro His Gly Gly Phe Val Ile Arg Ser 
                245                 250                 255     


Gly Asp Ser Gly Ala Asn Asp Lys Gln Ile Leu Glu Phe Ser Leu Lys 
            260                 265                 270         


Leu Asp Ser Asn Pro Asn Phe Thr Ser Ser Val Leu Val Ala Tyr Ala 
        275                 280                 285             


Arg Ala Ala His Arg Leu Ser Gln Ala Gly Asp Lys Gly Ala Lys Thr 
    290                 295                 300                 


Val Phe Asp Ile Pro Phe Ser Leu Leu Ser Pro Lys Ser Ala Ala Glu 
305                 310                 315                 320 


Leu Arg Lys Glu Leu Leu 
                325     


<210>  5
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Bacillus sphaericus

<400>  5

Met Ser Ala Ile Arg Val Gly Ile Val Gly Tyr Gly Asn Leu Gly Arg 
1               5                   10                  15      


Gly Val Glu Phe Ala Ile Ser Gln Asn Pro Asp Met Glu Leu Val Ala 
            20                  25                  30          


Val Phe Thr Arg Arg Asp Pro Ser Thr Val Ser Val Ala Ser Asn Ala 
        35                  40                  45              


Ser Val Tyr Leu Val Asp Asp Ala Glu Lys Phe Gln Asp Asp Ile Asp 
    50                  55                  60                  


Val Met Ile Leu Cys Gly Gly Ser Ala Thr Asp Leu Pro Glu Gln Gly 
65                  70                  75                  80  


Pro His Phe Ala Gln Trp Phe Asn Thr Ile Asp Ser Phe Asp Thr His 
                85                  90                  95      


Ala Lys Ile Pro Glu Phe Phe Asp Ala Val Asp Ala Ala Ala Gln Lys 
            100                 105                 110         


Ser Gly Lys Val Ser Val Ile Ser Val Gly Trp Asp Pro Gly Leu Phe 
        115                 120                 125             


Ser Leu Asn Arg Val Leu Gly Glu Ala Val Leu Pro Val Gly Thr Thr 
    130                 135                 140                 


Tyr Thr Phe Trp Gly Asp Gly Leu Ser Gln Gly His Ser Asp Ala Val 
145                 150                 155                 160 


Arg Arg Ile Glu Gly Val Lys Asn Ala Val Gln Tyr Thr Leu Pro Ile 
                165                 170                 175     


Lys Asp Ala Val Glu Arg Val Arg Asn Gly Glu Asn Pro Glu Leu Thr 
            180                 185                 190         


Thr Arg Glu Lys His Ala Arg Glu Cys Trp Val Val Leu Glu Glu Gly 
        195                 200                 205             


Ala Asp Ala Pro Lys Val Glu Gln Glu Ile Val Thr Met Pro Asn Tyr 
    210                 215                 220                 


Phe Asp Glu Tyr Asn Thr Thr Val Asn Phe Ile Ser Glu Asp Glu Phe 
225                 230                 235                 240 


Asn Ala Asn His Thr Gly Met Pro His Gly Gly Phe Val Ile Arg Ser 
                245                 250                 255     


Gly Glu Ser Gly Ala Asn Asp Lys Gln Ile Leu Glu Phe Ser Leu Lys 
            260                 265                 270         


Leu Glu Ser Asn Pro Asn Phe Thr Ser Ser Val Leu Val Ala Tyr Ala 
        275                 280                 285             


Arg Ala Ala His Arg Leu Ser Gln Ala Gly Glu Lys Gly Ala Lys Thr 
    290                 295                 300                 


Val Phe Asp Ile Pro Phe Gly Leu Leu Ser Pro Lys Ser Ala Ala Gln 
305                 310                 315                 320 


Leu Arg Lys Glu Leu Leu 
                325     


<210>  6
<211>  320
<212>  PRT
<213>  Cornybacterium glutamicum

<400>  6

Met Thr Asn Ile Arg Val Ala Ile Val Gly Tyr Gly Asn Leu Gly Arg 
1               5                   10                  15      


Ser Val Glu Lys Leu Ile Ala Lys Gln Pro Asp Met Asp Leu Val Gly 
            20                  25                  30          


Ile Phe Ser Arg Arg Ala Thr Leu Asp Thr Lys Thr Pro Val Phe Asp 
        35                  40                  45              


Val Ala Asp Val Asp Lys His Ala Asp Asp Val Asp Val Leu Phe Leu 
    50                  55                  60                  


Cys Met Gly Ser Ala Thr Asp Ile Pro Glu Gln Ala Pro Lys Phe Ala 
65                  70                  75                  80  


Gln Phe Ala Cys Thr Val Asp Thr Tyr Asp Asn His Arg Asp Ile Pro 
                85                  90                  95      


Arg His Arg Gln Val Met Asn Glu Ala Ala Thr Ala Ala Gly Asn Val 
            100                 105                 110         


Ala Leu Val Ser Thr Gly Trp Asp Pro Gly Met Phe Ser Ile Asn Arg 
        115                 120                 125             


Val Tyr Ala Ala Ala Val Leu Ala Glu His Gln Gln His Thr Phe Trp 
    130                 135                 140                 


Gly Pro Gly Leu Ser Gln Gly His Ser Asp Ala Leu Arg Arg Ile Pro 
145                 150                 155                 160 


Gly Val Gln Lys Ala Val Gln Tyr Thr Leu Pro Ser Glu Asp Ala Leu 
                165                 170                 175     


Glu Lys Ala Arg Arg Gly Glu Ala Gly Asp Leu Thr Gly Lys Gln Thr 
            180                 185                 190         


His Lys Arg Gln Cys Phe Val Val Ala Asp Ala Ala Asp His Glu Arg 
        195                 200                 205             


Ile Glu Asn Asp Ile Arg Thr Met Pro Asp Tyr Phe Val Gly Tyr Glu 
    210                 215                 220                 


Val Glu Val Asn Phe Ile Asp Glu Ala Thr Phe Asp Ser Glu His Thr 
225                 230                 235                 240 


Gly Met Pro His Gly Gly His Val Ile Thr Thr Gly Asp Thr Gly Gly 
                245                 250                 255     


Phe Asn His Thr Val Glu Tyr Ile Leu Lys Leu Asp Arg Asn Pro Asp 
            260                 265                 270         


Phe Thr Ala Ser Ser Gln Ile Ala Phe Gly Arg Ala Ala His Arg Met 
        275                 280                 285             


Lys Gln Gln Gly Gln Ser Gly Ala Phe Thr Val Leu Glu Val Ala Pro 
    290                 295                 300                 


Tyr Leu Leu Ser Pro Glu Asn Leu Asp Asp Leu Ile Ala Arg Asp Val 
305                 310                 315                 320 


<210>  7
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
ggggcgatgg cctaagtctg ggacattcag gcgctgttcg tcgtattgaa gg               52


<210>  8
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
aaaatgctgt tcaatacatt ttaccgatta aagaagcgg                              39


<210>  9
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  9
caacacgtga aaaacatgca atggaatgtt gggttgtatt agaag                       45


<210>  10
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  10
caaatcatac tggtatgcca aacggtggtt ttgtcattcg cagtgg                      46


<210>  11
<211>  326
<212>  PRT
<213>  Bacillus sphaericus

<400>  11

Met Ser Ala Ile Arg Val Gly Ile Val Gly Tyr Gly Asn Leu Gly Arg 
1               5                   10                  15      


Gly Val Glu Phe Ala Ile Ser Gln Asn Pro Asp Met Glu Leu Val Ala 
            20                  25                  30          


Val Phe Thr Arg Arg Asp Pro Ser Thr Val Ser Val Ala Ser Asn Ala 
        35                  40                  45              


Ser Val Tyr Leu Val Asp Asp Ala Glu Lys Phe Gln Asp Asp Ile Asp 
    50                  55                  60                  


Val Met Ile Leu Cys Gly Gly Ser Ala Thr Asp Leu Pro Glu Gln Gly 
65                  70                  75                  80  


Pro His Phe Ala Gln Trp Phe Asn Thr Ile Asp Ser Phe Asp Thr His 
                85                  90                  95      


Ala Lys Ile Pro Glu Phe Phe Asp Ala Val Asp Ala Ala Ala Gln Lys 
            100                 105                 110         


Ser Gly Lys Val Ser Val Ile Ser Val Gly Trp Asp Pro Gly Leu Phe 
        115                 120                 125             


Ser Leu Asn Arg Val Leu Gly Glu Ala Val Leu Pro Val Gly Thr Thr 
    130                 135                 140                 


Tyr Thr Phe Trp Gly Asp Gly Leu Ser Leu Gly His Ser Gly Ala Val 
145                 150                 155                 160 


Arg Arg Ile Glu Gly Val Lys Asn Ala Val Gln Tyr Ile Leu Pro Ile 
                165                 170                 175     


Lys Asp Ala Val Glu Arg Val Arg Asn Gly Glu Asn Pro Glu Leu Thr 
            180                 185                 190         


Thr Arg Glu Lys His Ala Met Glu Cys Trp Val Val Leu Glu Glu Gly 
        195                 200                 205             


Ala Asp Ala Pro Lys Val Glu Gln Glu Ile Val Thr Met Pro Asn Tyr 
    210                 215                 220                 


Phe Asp Glu Tyr Asn Thr Thr Val Asn Phe Ile Ser Glu Asp Glu Phe 
225                 230                 235                 240 


Asn Ala Asn His Thr Gly Met Pro Asn Gly Gly Phe Val Ile Arg Ser 
                245                 250                 255     


Gly Glu Ser Gly Ala Asn Asp Lys Gln Ile Leu Glu Phe Ser Leu Lys 
            260                 265                 270         


Leu Glu Ser Asn Pro Asn Phe Thr Ser Ser Val Leu Val Ala Tyr Ala 
        275                 280                 285             


Arg Ala Ala His Arg Leu Ser Gln Ala Gly Glu Lys Gly Ala Lys Thr 
    290                 295                 300                 


Val Phe Asp Ile Pro Phe Gly Leu Leu Ser Pro Lys Ser Ala Ala Gln 
305                 310                 315                 320 


Leu Arg Lys Glu Leu Leu 
                325     


<210>  12
<211>  990
<212>  DNA
<213>  Bacillus spaericus

<400>  12
catatgagtg caattcgagt aggtattgta ggatatggaa atttaggacg tggtgtggag       60

tatgccattt cacaaaatcc agatatggaa ttagtagcag tgttcacacg acgtgatcct      120

tcaacagtaa acgtagcaag caatgcgagc gtatatttag tagatgacgc tgaaaaattc      180

caaaacgaca ttgatgttat gattttatgt ggtggttctg caacagattt accagagcaa      240

ggtccacatt ttgcacaatg gtttaataca attgatagct tcgatacaca tgcgaaaatt      300

cctgaatttt tcgatgctgt agatgcagcg gcacaaaaat ctggtaaagt atctgttatc      360

tcagttggtt gggatccagg tctattctct ttaaatcgtc tacttggaga atcagtatta      420

ccagttggta caacatatac attctggggc gatggcctaa gtctgggaca ttcaggcgct      480

gttcgtcgta ttgaaggcgt aaaaaatgct gttcaataca ttttaccgat taaagaagcg      540

gtagaacgag ttcgtaacgg tgaaaaccct gaactaacaa cacgtgaaaa acatgcaatg      600

gaatgttggg ttgtattaga agaaggtgca gatgcaacaa aggttgaaca agaaattgtc      660

aaaatgccta actatttcga tgaatatgac acaacagtga acttcatttc tgaagatgaa      720

tttaaagcaa atcatactgg tatgccaaac ggtggttttg tcattcgcag tggtgacagt      780

ggtgcaaacg ataaacaaat tctcgaattc tcattaaaac tggacagtaa tccaaacttt      840

acatctagtg tattagtggc ttatgcacgt gcagcacacc gtttaagtca agcgggtgat      900

aaaggtgcga aaacagtatt tgatatccca ttcagcctgc tttcgccaaa atcagctgct      960

gaattacgta aggaactatt ataaggatcc                                       990


<210>  13
<211>  798
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gluconobacter Oxydans

<400>  13
atgcctgccc cttacaaaga ccgtttcgcc ggcaagaaag tcctcgtcac cggggcctcc       60

cagggaattg gcgaggccac cgcgcttcgt tttgccgaag aaggcgcgca ggtcgccctc      120

aacggccgca aggaagacaa gctgatcgcc gtccgcgaga agctgcccaa ggtttccggc      180

ggagagcacc cgatcgccac gggtgacatt tccaaagaag acgacgtcaa acgtctggtt      240

gccgagagca tcaaggccat gggtggtctc gacgttctgg tctgcaatgc gggctatcag      300

atcccctccc cgtcagaaga catcaagctc gaagattttg aaggcgtgat ggccgtcaac      360

gtcacggggg tgatgctgcc ctgtcgcgaa gtcatccgct actggctgga aaacggcatc      420

aagggcacga tcattgtgaa ctcctccgtt caccagatca tcccgaaacc gcattatctg      480

ggctattccg cctccaaggg tgccgtcggc aacattgtcc gcacgctggc actggaatat      540

gccacccgcg gcatccgggt gaatgccgtg gcgcccggcg ccatcgtgac gccgatcaac      600

atgtcgtgga tcgacgatcc cgaacagtac aaggccgttt cgagccacat cccgatgaag      660

cgcccgggcg aaagccgcga aatcgcggat gccatcacct tcctcgccgc cgaggacagc      720

acctacatca cgggtcagac cctgtatgtc gatggtggtc tgacgctcta cggcgatttc      780

gaaaacaact ggtcctcg                                                    798


<210>  14
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gluconobacter oxydans

<400>  14

Met Pro Ala Pro Tyr Lys Asp Arg Phe Ala Gly Lys Lys Val Leu Val 
1               5                   10                  15      


Thr Gly Ala Ser Gln Gly Ile Gly Glu Ala Thr Ala Leu Arg Phe Ala 
            20                  25                  30          


Glu Glu Gly Ala Gln Val Ala Leu Asn Gly Arg Lys Glu Asp Lys Leu 
        35                  40                  45              


Ile Ala Val Arg Glu Lys Leu Pro Lys Val Ser Gly Gly Glu His Pro 
    50                  55                  60                  


Ile Ala Thr Gly Asp Ile Ser Lys Glu Asp Asp Val Lys Arg Leu Val 
65                  70                  75                  80  


Ala Glu Ser Ile Lys Ala Met Gly Gly Leu Asp Val Leu Val Cys Asn 
                85                  90                  95      


Ala Gly Tyr Gln Ile Pro Ser Pro Ser Glu Asp Ile Lys Leu Glu Asp 
            100                 105                 110         


Phe Glu Gly Val Met Ala Val Asn Val Thr Gly Val Met Leu Pro Cys 
        115                 120                 125             


Arg Glu Val Ile Arg Tyr Trp Leu Glu Asn Gly Ile Lys Gly Thr Ile 
    130                 135                 140                 


Ile Val Asn Ser Ser Val His Gln Ile Ile Pro Lys Pro His Tyr Leu 
145                 150                 155                 160 


Gly Tyr Ser Ala Ser Lys Gly Ala Val Gly Asn Ile Val Arg Thr Leu 
                165                 170                 175     


Ala Leu Glu Tyr Ala Thr Arg Gly Ile Arg Val Asn Ala Val Ala Pro 
            180                 185                 190         


Gly Ala Ile Val Thr Pro Ile Asn Met Ser Trp Ile Asp Asp Pro Glu 
        195                 200                 205             


Gln Tyr Lys Ala Val Ser Ser His Ile Pro Met Lys Arg Pro Gly Glu 
    210                 215                 220                 


Ser Arg Glu Ile Ala Asp Ala Ile Thr Phe Leu Ala Ala Glu Asp Ser 
225                 230                 235                 240 


Thr Tyr Ile Thr Gly Gln Thr Leu Tyr Val Asp Gly Gly Leu Thr Leu 
                245                 250                 255     


Tyr Gly Asp Phe Glu Asn Asn Trp Ser Ser 
            260                 265     


<210>  15
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-terminal primer

<400>  15
agccttggta cctaatacca ggaataaata aaacatatga gtgcaattcg agtaggtatt       60

gtagg                                                                   65


<210>  16
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C-terminal primer

<400>  16
gcaggtacct tataatagtt ccttacg                                           27


<210>  17
<211>  42
<212>  DNA
<213>  E. Coli

<400>  17
gatttcgaaa acaactggtc ctcgtaaggt acctaatacc ag                          42


<210>  18
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E.Coli

<400>  18

Asp Phe Glu Asn Asn Trp Ser Ser 
1               5               


<210>  19
<211>  36
<212>  DNA
<213>  E coli

<400>  19
gaataaataa aacatatgag tgcaattcga gtaggt                                 36


<210>  20
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E. Coli

<400>  20

Met Ser Ala Ile Arg Val Gly 
1               5           


<210>  21
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-terminal primer

<400>  21
atggatcaga catattctct gg                                                22


<210>  22
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C-terminal primer

<400>  22
aatttagtgt gggacgcggt cg                                                22


<210>  23
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  23
aaaaacttat aattatcctt aaaagacttt ggtgtgcgcc cagatagggt g                51


<210>  24
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  24
cagattgtac aaatgtggtg ataacagata agtctttggt cttaacttac ctttctttgt       60


<210>  25
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  25
tgaacgcaag tttctaattt cggtttcttt ccgatagagg aaagtgtct                   49


<210>  26
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  26
gacatattta aatcatatgc ctgcccctta caaagaccg                              39


<210>  27
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  27
gacatttaaa tggatcctta cgaggaccag ttgttttcg                              39


<210>  28
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Bacillus sphaericus

<400>  28

Arg Lys Glu Leu Leu 
1               5   


