
                                SEQUENCE LISTING

<110> Kolltan Pharmaceuticals, Inc.

<120> ANTI-KIT ANTIBODIES AND USES THEREOF  

<130> 12638-028-228

<140>
<141>

<150> 61/436,483          
<151> 2011-01-26  

<150> 61/507,430          
<151> 2011-07-13  

<150> 61/537,482          
<151> 2011-09-21  

<160> 49

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 972
<212> PRT
<213> Homo sapiens

<220> 
<223> full length human KIT (Genbank accession no. AAC50969)

<400> 1
Met Arg Gly Ala Arg Gly Ala Trp Asp Phe Leu Cys Val Leu Leu Leu
 1               5                  10                  15      
Leu Leu Arg Val Gln Thr Gly Ser Ser Gln Pro Ser Val Ser Pro Gly
            20                  25                  30          
Glu Pro Ser Pro Pro Ser Ile His Pro Gly Lys Ser Asp Leu Ile Val
        35                  40                  45              
Arg Val Gly Asp Glu Ile Arg Leu Leu Cys Thr Asp Pro Gly Phe Val
    50                  55                  60                  
Lys Trp Thr Phe Glu Ile Leu Asp Glu Thr Asn Glu Asn Lys Gln Asn
65                  70                  75                  80  
Glu Trp Ile Thr Glu Lys Ala Glu Ala Thr Asn Thr Gly Lys Tyr Thr
                85                  90                  95      
Cys Thr Asn Lys His Gly Leu Ser Asn Ser Ile Tyr Val Phe Val Arg
            100                 105                 110         
Asp Pro Ala Lys Leu Phe Leu Val Asp Arg Ser Leu Tyr Gly Lys Glu
        115                 120                 125             
Asp Asn Asp Thr Leu Val Arg Cys Pro Leu Thr Asp Pro Glu Val Thr
    130                 135                 140                 
Asn Tyr Ser Leu Lys Gly Cys Gln Gly Lys Pro Leu Pro Lys Asp Leu
145                 150                 155                 160 
Arg Phe Ile Pro Asp Pro Lys Ala Gly Ile Met Ile Lys Ser Val Lys
                165                 170                 175     
Arg Ala Tyr His Arg Leu Cys Leu His Cys Ser Val Asp Gln Glu Gly
            180                 185                 190         
Lys Ser Val Leu Ser Glu Lys Phe Ile Leu Lys Val Arg Pro Ala Phe
        195                 200                 205             
Lys Ala Val Pro Val Val Ser Val Ser Lys Ala Ser Tyr Leu Leu Arg
    210                 215                 220                 
Glu Gly Glu Glu Phe Thr Val Thr Cys Thr Ile Lys Asp Val Ser Ser
225                 230                 235                 240 
Ser Val Tyr Ser Thr Trp Lys Arg Glu Asn Ser Gln Thr Lys Leu Gln
                245                 250                 255     
Glu Lys Tyr Asn Ser Trp His His Gly Asp Phe Asn Tyr Glu Arg Gln
            260                 265                 270         
Ala Thr Leu Thr Ile Ser Ser Ala Arg Val Asn Asp Ser Gly Val Phe
        275                 280                 285             
Met Cys Tyr Ala Asn Asn Thr Phe Gly Ser Ala Asn Val Thr Thr Thr
    290                 295                 300                 
Leu Glu Val Val Asp Lys Gly Phe Ile Asn Ile Phe Pro Met Ile Asn
305                 310                 315                 320 
Thr Thr Val Phe Val Asn Asp Gly Glu Asn Val Asp Leu Ile Val Glu
                325                 330                 335     
Tyr Glu Ala Phe Pro Lys Pro Glu His Gln Gln Trp Ile Tyr Met Asn
            340                 345                 350         
Arg Thr Phe Thr Asp Lys Trp Glu Asp Tyr Pro Lys Ser Glu Asn Glu
        355                 360                 365             
Ser Asn Ile Arg Tyr Val Ser Glu Leu His Leu Thr Arg Leu Lys Gly
    370                 375                 380                 
Thr Glu Gly Gly Thr Tyr Thr Phe Leu Val Ser Asn Ser Asp Val Asn
385                 390                 395                 400 
Ala Ala Ile Ala Phe Asn Val Tyr Val Asn Thr Lys Pro Glu Ile Leu
                405                 410                 415     
Thr Tyr Asp Arg Leu Val Asn Gly Met Leu Gln Cys Val Ala Ala Gly
            420                 425                 430         
Phe Pro Glu Pro Thr Ile Asp Trp Tyr Phe Cys Pro Gly Thr Glu Gln
        435                 440                 445             
Arg Cys Ser Ala Ser Val Leu Pro Val Asp Val Gln Thr Leu Asn Ser
    450                 455                 460                 
Ser Gly Pro Pro Phe Gly Lys Leu Val Val Gln Ser Ser Ile Asp Ser
465                 470                 475                 480 
Ser Ala Phe Lys His Asn Gly Thr Val Glu Cys Lys Ala Tyr Asn Asp
                485                 490                 495     
Val Gly Lys Thr Ser Ala Tyr Phe Asn Phe Ala Phe Lys Glu Gln Ile
            500                 505                 510         
His Pro His Thr Leu Phe Thr Pro Leu Leu Ile Gly Phe Val Ile Val
        515                 520                 525             
Ala Gly Met Met Cys Ile Ile Val Met Ile Leu Thr Tyr Lys Tyr Leu
    530                 535                 540                 
Gln Lys Pro Met Tyr Glu Val Gln Trp Lys Val Val Glu Glu Ile Asn
545                 550                 555                 560 
Gly Asn Asn Tyr Val Tyr Ile Asp Pro Thr Gln Leu Pro Tyr Asp His
                565                 570                 575     
Lys Trp Glu Phe Pro Arg Asn Arg Leu Ser Phe Gly Lys Thr Leu Gly
            580                 585                 590         
Ala Gly Ala Phe Gly Lys Val Val Glu Ala Thr Ala Tyr Gly Leu Ile
        595                 600                 605             
Lys Ser Asp Ala Ala Met Thr Val Ala Val Lys Met Leu Lys Pro Ser
    610                 615                 620                 
Ala His Leu Thr Glu Arg Glu Ala Leu Met Ser Glu Leu Lys Val Leu
625                 630                 635                 640 
Ser Tyr Leu Gly Asn His Met Asn Ile Val Asn Leu Leu Gly Ala Cys
                645                 650                 655     
Thr Ile Gly Gly Pro Thr Leu Val Ile Thr Glu Tyr Cys Cys Tyr Gly
            660                 665                 670         
Asp Leu Leu Asn Phe Leu Arg Arg Lys Arg Asp Ser Phe Ile Cys Ser
        675                 680                 685             
Lys Gln Glu Asp His Ala Glu Ala Ala Leu Tyr Lys Asn Leu Leu His
    690                 695                 700                 
Ser Lys Glu Ser Ser Cys Ser Asp Ser Thr Asn Glu Tyr Met Asp Met
705                 710                 715                 720 
Lys Pro Gly Val Ser Tyr Val Val Pro Thr Lys Ala Asp Lys Arg Arg
                725                 730                 735     
Ser Val Arg Ile Gly Ser Tyr Ile Glu Arg Asp Val Thr Pro Ala Ile
            740                 745                 750         
Met Glu Asp Asp Glu Leu Ala Leu Asp Leu Glu Asp Leu Leu Ser Phe
        755                 760                 765             
Ser Tyr Gln Val Ala Lys Gly Met Ala Phe Leu Ala Ser Lys Asn Cys
    770                 775                 780                 
Ile His Arg Asp Leu Ala Ala Arg Asn Ile Leu Leu Thr His Gly Arg
785                 790                 795                 800 
Ile Thr Lys Ile Cys Asp Phe Gly Leu Ala Arg Asp Ile Lys Asn Asp
                805                 810                 815     
Ser Asn Tyr Val Val Lys Gly Asn Ala Arg Leu Pro Val Lys Trp Met
            820                 825                 830         
Ala Pro Glu Ser Ile Phe Asn Cys Val Tyr Thr Phe Glu Ser Asp Val
        835                 840                 845             
Trp Ser Tyr Gly Ile Phe Leu Trp Glu Leu Phe Ser Leu Gly Ser Ser
    850                 855                 860                 
Pro Tyr Pro Gly Met Pro Val Asp Ser Lys Phe Tyr Lys Met Ile Lys
865                 870                 875                 880 
Glu Gly Phe Arg Met Leu Ser Pro Glu His Ala Pro Ala Glu Met Tyr
                885                 890                 895     
Asp Ile Met Lys Thr Cys Trp Asp Ala Asp Pro Leu Lys Arg Pro Thr
            900                 905                 910         
Phe Lys Gln Ile Val Gln Leu Ile Glu Lys Gln Ile Ser Glu Ser Thr
        915                 920                 925             
Asn His Ile Tyr Ser Asn Leu Ala Asn Cys Ser Pro Asn Arg Gln Lys
    930                 935                 940                 
Pro Val Val Asp His Ser Val Arg Ile Asn Ser Val Gly Ser Thr Ala
945                 950                 955                 960 
Ser Ser Ser Gln Pro Leu Leu Val His Asp Asp Val
                965                 970         


<210> 2
<211> 108
<212> PRT
<213> mouse

<220> 
<223> variable light chain region of anti-KIT antibodies 37M and 37C 

<400> 2
Asp Ile Val Met Thr Gln Ser Gln Lys Phe Met Ser Thr Ser Val Gly
 1               5                  10                  15      
Asp Arg Val Ser Val Thr Cys Lys Ala Ser Gln Asn Val Arg Thr Asn
            20                  25                  30          
Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Ala Leu Ile
        35                  40                  45              
Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Asp Arg Phe Thr Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Asn Val Gln Ser
65                  70                  75                  80  
Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr Asn Ser Tyr Pro Arg
                85                  90                  95      
Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg
            100                 105             


<210> 3
<211> 116
<212> PRT
<213> mouse

<220> 
<223> variable heavy chain region of anti-KIT antibody 37M

<400> 3
Gln Val Gln Leu Lys Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala
 1               5                  10                  15      
Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr
            20                  25                  30          
Tyr Ile Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile
        35                  40                  45              
Ala Arg Ile Tyr Pro Gly Ser Gly Asn Thr Tyr Tyr Asn Glu Lys Phe
    50                  55                  60                  
Lys Gly Lys Ala Thr Leu Thr Ala Glu Lys Ser Ser Ser Thr Ala Tyr
65                  70                  75                  80  
Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys
                85                  90                  95      
Ala Arg Gly Val Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu
            100                 105                 110         
Thr Val Ser Ser
        115     


<210> 4

<400> 4
000                                                               3

<210> 5
<211> 116
<212> PRT
<213> Artificial Sequence

<220> 
<223> variable heavy chain region of anti-KIT antibody 37C

<400> 5
Gln Val Gln Leu Lys Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala
 1               5                  10                  15      
Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr
            20                  25                  30          
Tyr Ile Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile
        35                  40                  45              
Ala Arg Ile Tyr Pro Gly Ser Gly Asn Thr Tyr Tyr Asn Glu Lys Phe
    50                  55                  60                  
Lys Gly Lys Ala Thr Leu Thr Ala Glu Lys Ser Ser Ser Thr Ala Tyr
65                  70                  75                  80  
Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys
                85                  90                  95      
Ala Arg Gly Val Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu
            100                 105                 110         
Thr Val Ser Ala
        115     


<210> 6
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> light chain of antibody 37C

<400> 6
Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly
 1               5                  10                  15      
Val His Ser Asp Ile Val Met Thr Gln Ser Gln Lys Phe Met Ser Thr
            20                  25                  30          
Ser Val Gly Asp Arg Val Ser Val Thr Cys Lys Ala Ser Gln Asn Val
        35                  40                  45              
Arg Thr Asn Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys
    50                  55                  60                  
Ala Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Asp Arg
65                  70                  75                  80  
Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Asn
                85                  90                  95      
Val Gln Ser Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr Asn Ser
            100                 105                 110         
Tyr Pro Arg Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Thr
        115                 120                 125             
Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu
    130                 135                 140                 
Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro
145                 150                 155                 160 
Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly
                165                 170                 175     
Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr
            180                 185                 190         
Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His
        195                 200                 205             
Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val
    210                 215                 220                 
Thr Lys Ser Phe Asn Arg Gly Glu Cys
225                 230             


<210> 7
<211> 465
<212> PRT
<213> Artificial Sequence

<220> 
<223> heavy chain of antibody 37C

<400> 7
Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly
 1               5                  10                  15      
Val His Ser Gln Val Gln Leu Lys Gln Ser Gly Ala Glu Leu Val Arg
            20                  25                  30          
Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe
        35                  40                  45              
Thr Asp Tyr Tyr Ile Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu
    50                  55                  60                  
Glu Trp Ile Ala Arg Ile Tyr Pro Gly Ser Gly Asn Thr Tyr Tyr Asn
65                  70                  75                  80  
Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Glu Lys Ser Ser Ser
                85                  90                  95      
Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val
            100                 105                 110         
Tyr Phe Cys Ala Arg Gly Val Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly
        115                 120                 125             
Thr Thr Leu Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe
    130                 135                 140                 
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu
145                 150                 155                 160 
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp
                165                 170                 175     
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu
            180                 185                 190         
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser
        195                 200                 205             
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro
    210                 215                 220                 
Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys
225                 230                 235                 240 
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro
                245                 250                 255     
Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser
            260                 265                 270         
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp
        275                 280                 285             
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn
    290                 295                 300                 
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val
305                 310                 315                 320 
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu
                325                 330                 335     
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys
            340                 345                 350         
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr
        355                 360                 365             
Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr
    370                 375                 380                 
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu
385                 390                 395                 400 
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu
                405                 410                 415     
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys
            420                 425                 430         
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu
        435                 440                 445             
Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly
    450                 455                 460                 
Lys
465 


<210> 8
<211> 324
<212> DNA
<213> Artificial Sequence

<220> 
<223> sequence encoding VL domain of anti-KIT antibody 37M 

<400> 8
gacattgtga tgacccagtc tcaaaaattc atgtccacat cagtaggaga cagggtcagc 60
gtcacctgca aggccagtca gaatgtgcgt actaatgtag cctggtatca acagaaacca 120
gggcaatctc ctaaagcact gatttactcg gcatcctacc ggtacagtgg agtccctgat 180
cgcttcacag gcagtggatc tgggacagat ttcactctca ccatcagcaa tgtgcagtct 240
gaagacttgg cagactattt ctgtcagcaa tataacagct atcctcggac gttcggtgga 300
ggcaccaagc tggaaatcaa acgt                                        324

<210> 9
<211> 348
<212> DNA
<213> Artificial Sequence

<220> 
<223> sequence encoding VH domain of anti-KIT antibody 37M 

<400> 9
caggtccagc tgaagcagtc tggggctgag ctggtgaggc ctggggcctc agtgaagctg 60
tcctgcaagg cttctggcta cactttcact gactactata taaactgggt gaagcagagg 120
cctggacagg gacttgagtg gattgcaagg atttaccctg gaagtggtaa tacttactac 180
aatgagaagt tcaagggcaa ggccacactg actgcagaaa aatcctccag cactgcctac 240
atgcagctca gcagcctgac atctgaggac tctgctgtct atttctgtgc aaggggggtg 300
tactactttg actactgggg ccaaggcacc actctcacag tctcctca              348

<210> 10
<211> 702
<212> DNA
<213> Artificial Sequence

<220> 
<223> sequence encoding the light chain of antibody 37C 

<400> 10
atgggctgga gctgcatcat cctgttcctg gtggccaccg ccaccggtgt gcacagcgac 60
attgtgatga cccagtctca aaaattcatg tccacatcag taggagacag ggtcagcgtc 120
acctgcaagg ccagtcagaa tgtgcgtact aatgtagcct ggtatcaaca gaaaccaggg 180
caatctccta aagcactgat ttactcggca tcctaccggt acagtggagt ccctgatcgc 240
ttcacaggca gtggatctgg gacagatttc actctcacca tcagcaatgt gcagtctgaa 300
gacttggcag actatttctg tcagcaatat aacagctatc ctcggacgtt cggtggaggc 360
accaagctcg agatcaagag aaccgtggcc gcccccagcg tgttcatctt cccccccagc 420
gacgagcagc tgaagagcgg caccgccagc gtggtgtgcc tgctgaacaa cttctacccc 480
agagaggcca aggtgcagtg gaaggtggac aacgccctgc agagcggcaa cagccaggag 540
agcgtgaccg agcaggacag caaggacagc acctacagcc tgagcagcac cctgaccctg 600
agcaaggccg actacgagaa gcacaaggtg tacgcctgcg aggtgaccca ccagggcctg 660
agcagccccg tgaccaagag cttcaacaga ggcgagtgct ga                    702

<210> 11
<211> 1398
<212> DNA
<213> Artificial Sequence

<220> 
<223> sequence encoding the heavy chain of antibody 37C 

<400> 11
atgggctgga gctgcatcat cctgttcctg gtggccaccg ccaccggcgt gcacagccag 60
gtgcaattga agcagtctgg ggctgagctg gtgaggcctg gggcctcagt gaagctgtcc 120
tgcaaggctt ctggctacac tttcactgac tactatataa actgggtgaa gcagaggcct 180
ggacagggac ttgagtggat tgcaaggatt taccctggaa gtggtaatac ttactacaat 240
gagaagttca agggcaaggc cacactgact gcagaaaaat cctccagcac tgcctacatg 300
cagctcagca gcctgacatc tgaggactct gctgtctatt tctgtgcaag gggggtgtac 360
tactttgact actggggcca aggcaccact ctcacagtct ccgcggccag cactaagggc 420
cccagcgtgt tcccgctagc ccccagcagc aagagcacca gcggcggcac cgccgccctg 480
ggctgcctgg tgaaggacta cttccccgag cccgtgaccg tgagctggaa cagcggcgcc 540
ctgaccagcg gcgtgcacac cttccccgcc gtgctgcaga gcagcggcct gtacagcctg 600
agcagcgtgg tgaccgtgcc cagcagcagc ctgggcaccc agacctacat ctgcaacgtg 660
aaccacaagc ccagcaacac caaggtggac aagaaggtgg agcccaagag ctgcgacaag 720
acccacacct gccccccctg ccccgccccc gagctgctgg gcggccccag cgtgttcctg 780
ttccccccca agcccaagga caccctgatg atcagcagaa cccccgaggt gacctgcgtg 840
gtggtggacg tgagccacga ggaccccgag gtgaagttca actggtacgt ggacggcgtg 900
gaggtgcaca acgccaagac caagcccaga gaggagcagt acaacagcac ctacagagtg 960
gtgagcgtgc tgaccgtgct gcaccaggac tggctgaacg gcaaggagta caagtgcaag 1020
gtgagcaaca aggccctgcc cgcccccatc gagaagacca tcagcaaggc caagggccag 1080
cccagagagc cccaggtgta caccctgccc cccagcagag acgagctgac caagaaccag 1140
gtgagcctga cctgcctggt gaagggcttc taccccagcg acatcgccgt ggagtgggag 1200
agcaacggcc agcccgagaa caactacaag accacccccc ccgtgctgga cagcgacggc 1260
agcttcttcc tgtacagcaa gctgaccgtg gacaagagca gatggcagca gggcaacgtg 1320
ttcagctgca gcgtgatgca cgaggccctg cacaaccact acacccagaa gagcctgagc 1380
ctgagccccg gcaagtag                                               1398

<210> 12
<211> 106
<212> PRT
<213> Artificial Sequence

<220> 
<223> light chain constant domain of antibody 37C 

<400> 12
Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln
 1               5                  10                  15      
Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr
            20                  25                  30          
Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser
        35                  40                  45              
Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr
    50                  55                  60                  
Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys
65                  70                  75                  80  
His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro
                85                  90                  95      
Val Thr Lys Ser Phe Asn Arg Gly Glu Cys
            100                 105     


<210> 13
<211> 330
<212> PRT
<213> Artificial Sequence

<220> 
<223> heavy chain constant domain of antibody 37C 

<400> 13
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys
 1               5                  10                  15      
Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr
            20                  25                  30          
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
        35                  40                  45              
Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser
    50                  55                  60                  
Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr
65                  70                  75                  80  
Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys
                85                  90                  95      
Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys
            100                 105                 110         
Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro
        115                 120                 125             
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys
    130                 135                 140                 
Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp
145                 150                 155                 160 
Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu
                165                 170                 175     
Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu
            180                 185                 190         
His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn
        195                 200                 205             
Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly
    210                 215                 220                 
Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
225                 230                 235                 240 
Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
                245                 250                 255     
Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
            260                 265                 270         
Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
        275                 280                 285             
Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn
    290                 295                 300                 
Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr
305                 310                 315                 320 
Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
                325                 330 


<210> 14
<211> 246
<212> PRT
<213> Artificial Sequence

<220> 
<223> recombinant KIT D4/D5 polypeptide 

<400> 14
Met Arg Gly Ala Arg Gly Ala Trp Asp Phe Leu Cys Val Leu Leu Leu
 1               5                  10                  15      
Leu Leu Arg Val Gln Thr Gly Ser Ser Gln Pro Ser Val Ser Pro Gly
            20                  25                  30          
Glu Val Asp Lys Gly Phe Ile Asn Ile Phe Pro Met Ile Asn Thr Thr
        35                  40                  45              
Val Phe Val Asn Asp Gly Glu Asn Val Asp Leu Ile Val Glu Tyr Glu
    50                  55                  60                  
Ala Phe Pro Lys Pro Glu His Gln Gln Trp Ile Tyr Met Asn Arg Thr
65                  70                  75                  80  
Phe Thr Asp Lys Trp Glu Asp Tyr Pro Lys Ser Glu Asn Glu Ser Asn
                85                  90                  95      
Ile Arg Tyr Val Ser Glu Leu His Leu Thr Arg Leu Lys Gly Thr Glu
            100                 105                 110         
Gly Gly Thr Tyr Thr Phe Leu Val Ser Asn Ser Asp Val Asn Ala Ala
        115                 120                 125             
Ile Ala Phe Asn Val Tyr Val Asn Thr Lys Pro Glu Ile Leu Thr Tyr
    130                 135                 140                 
Asp Arg Leu Val Asn Gly Met Leu Gln Cys Val Ala Ala Gly Phe Pro
145                 150                 155                 160 
Glu Pro Thr Ile Asp Trp Tyr Phe Cys Pro Gly Thr Glu Gln Arg Cys
                165                 170                 175     
Ser Ala Ser Val Leu Pro Val Asp Val Gln Thr Leu Asn Ser Ser Gly
            180                 185                 190         
Pro Pro Phe Gly Lys Leu Val Val Gln Ser Ser Ile Asp Ser Ser Ala
        195                 200                 205             
Phe Lys His Asn Gly Thr Val Glu Cys Lys Ala Tyr Asn Asp Val Gly
    210                 215                 220                 
Lys Thr Ser Ala Tyr Phe Asn Phe Ala Phe Lys Glu Gln Ile His Pro
225                 230                 235                 240 
His His His His His His
                245     


<210> 15
<211> 208
<212> PRT
<213> Homo spiens

<220> 
<223> V308 to H515 of Human KIT amino acids sequence (KIT D4/D5 region)

<400> 15
Val Asp Lys Gly Phe Ile Asn Ile Phe Pro Met Ile Asn Thr Thr Val
 1               5                  10                  15      
Phe Val Asn Asp Gly Glu Asn Val Asp Leu Ile Val Glu Tyr Glu Ala
            20                  25                  30          
Phe Pro Lys Pro Glu His Gln Gln Trp Ile Tyr Met Asn Arg Thr Phe
        35                  40                  45              
Thr Asp Lys Trp Glu Asp Tyr Pro Lys Ser Glu Asn Glu Ser Asn Ile
    50                  55                  60                  
Arg Tyr Val Ser Glu Leu His Leu Thr Arg Leu Lys Gly Thr Glu Gly
65                  70                  75                  80  
Gly Thr Tyr Thr Phe Leu Val Ser Asn Ser Asp Val Asn Ala Ala Ile
                85                  90                  95      
Ala Phe Asn Val Tyr Val Asn Thr Lys Pro Glu Ile Leu Thr Tyr Asp
            100                 105                 110         
Arg Leu Val Asn Gly Met Leu Gln Cys Val Ala Ala Gly Phe Pro Glu
        115                 120                 125             
Pro Thr Ile Asp Trp Tyr Phe Cys Pro Gly Thr Glu Gln Arg Cys Ser
    130                 135                 140                 
Ala Ser Val Leu Pro Val Asp Val Gln Thr Leu Asn Ser Ser Gly Pro
145                 150                 155                 160 
Pro Phe Gly Lys Leu Val Val Gln Ser Ser Ile Asp Ser Ser Ala Phe
                165                 170                 175     
Lys His Asn Gly Thr Val Glu Cys Lys Ala Tyr Asn Asp Val Gly Lys
            180                 185                 190         
Thr Ser Ala Tyr Phe Asn Phe Ala Phe Lys Glu Gln Ile His Pro His
        195                 200                 205             


<210> 16
<211> 144
<212> PRT
<213> Artificial Sequence

<220> 
<223> recombinant D4 domain polypeptide 

<400> 16
Met Arg Gly Ala Arg Gly Ala Trp Asp Phe Leu Cys Val Leu Leu Leu
 1               5                  10                  15      
Leu Leu Arg Val Gln Thr Gly Ser Ser Gln Pro Ser Val Ser Pro Gly
            20                  25                  30          
Glu Val Asp Lys Gly Phe Ile Asn Ile Phe Pro Met Ile Asn Thr Thr
        35                  40                  45              
Val Phe Val Asn Asp Gly Glu Asn Val Asp Leu Ile Val Glu Tyr Glu
    50                  55                  60                  
Ala Phe Pro Lys Pro Glu His Gln Gln Trp Ile Tyr Met Asn Arg Thr
65                  70                  75                  80  
Phe Thr Asp Lys Trp Glu Asp Tyr Pro Lys Ser Glu Asn Glu Ser Asn
                85                  90                  95      
Ile Arg Tyr Val Ser Glu Leu His Leu Thr Arg Leu Lys Gly Thr Glu
            100                 105                 110         
Gly Gly Thr Tyr Thr Phe Leu Val Ser Asn Ser Asp Val Asn Ala Ala
        115                 120                 125             
Ile Ala Phe Asn Val Tyr Val Asn Thr Lys His His His His His His
    130                 135                 140                 


<210> 17
<211> 105
<212> PRT
<213> Homo sapiens

<220> 
<223> V308 to K412 of Human KIT amino acids sequence

<400> 17
Val Asp Lys Gly Phe Ile Asn Ile Phe Pro Met Ile Asn Thr Thr Val
 1               5                  10                  15      
Phe Val Asn Asp Gly Glu Asn Val Asp Leu Ile Val Glu Tyr Glu Ala
            20                  25                  30          
Phe Pro Lys Pro Glu His Gln Gln Trp Ile Tyr Met Asn Arg Thr Phe
        35                  40                  45              
Thr Asp Lys Trp Glu Asp Tyr Pro Lys Ser Glu Asn Glu Ser Asn Ile
    50                  55                  60                  
Arg Tyr Val Ser Glu Leu His Leu Thr Arg Leu Lys Gly Thr Glu Gly
65                  70                  75                  80  
Gly Thr Tyr Thr Phe Leu Val Ser Asn Ser Asp Val Asn Ala Ala Ile
                85                  90                  95      
Ala Phe Asn Val Tyr Val Asn Thr Lys
            100                 105 


<210> 18
<211> 148
<212> PRT
<213> Artificial Sequence

<220> 
<223> recombinant D5 domain polypeptide 

<400> 18
Met Arg Gly Ala Arg Gly Ala Trp Asp Phe Leu Cys Val Leu Leu Leu
 1               5                  10                  15      
Leu Leu Arg Val Gln Thr Gly Ser Ser Gln Pro Ser Val Ser Pro Gly
            20                  25                  30          
Glu Asn Thr Lys Pro Glu Ile Leu Thr Tyr Asp Arg Leu Val Asn Gly
        35                  40                  45              
Met Leu Gln Cys Val Ala Ala Gly Phe Pro Glu Pro Thr Ile Asp Trp
    50                  55                  60                  
Tyr Phe Cys Pro Gly Thr Glu Gln Arg Cys Ser Ala Ser Val Leu Pro
65                  70                  75                  80  
Val Asp Val Gln Thr Leu Asn Ser Ser Gly Pro Pro Phe Gly Lys Leu
                85                  90                  95      
Val Val Gln Ser Ser Ile Asp Ser Ser Ala Phe Lys His Asn Gly Thr
            100                 105                 110         
Val Glu Cys Lys Ala Tyr Asn Asp Val Gly Lys Thr Ser Ala Tyr Phe
        115                 120                 125             
Asn Phe Ala Phe Lys Gly Asn Asn Lys Glu Gln Ile His Pro His His
    130                 135                 140                 
His His His His
145             


<210> 19
<211> 110
<212> PRT
<213> Homo sapiens

<220> 
<223> N410 to H515 of Human KIT amino acids sequence

<400> 19
Asn Thr Lys Pro Glu Ile Leu Thr Tyr Asp Arg Leu Val Asn Gly Met
 1               5                  10                  15      
Leu Gln Cys Val Ala Ala Gly Phe Pro Glu Pro Thr Ile Asp Trp Tyr
            20                  25                  30          
Phe Cys Pro Gly Thr Glu Gln Arg Cys Ser Ala Ser Val Leu Pro Val
        35                  40                  45              
Asp Val Gln Thr Leu Asn Ser Ser Gly Pro Pro Phe Gly Lys Leu Val
    50                  55                  60                  
Val Gln Ser Ser Ile Asp Ser Ser Ala Phe Lys His Asn Gly Thr Val
65                  70                  75                  80  
Glu Cys Lys Ala Tyr Asn Asp Val Gly Lys Thr Ser Ala Tyr Phe Asn
                85                  90                  95      
Phe Ala Phe Lys Gly Asn Asn Lys Glu Gln Ile His Pro His
            100                 105                 110 


<210> 20
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL CDR1 of antibody 37M and 37C

<400> 20
Lys Ala Ser Gln Asn Val Arg Thr Asn Val Ala
 1               5                  10      


<210> 21
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL CDR2 of antibody 37M and 37C

<400> 21
Ser Ala Ser Tyr Arg Tyr Ser
 1               5          


<210> 22
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL CDR3 of antibody 37M and 37C

<400> 22
Gln Gln Tyr Asn Ser Tyr Pro Arg Thr
 1               5                  


<210> 23
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH CDR1 of antibody 37M and 37C

<400> 23
Asp Tyr Tyr Ile Asn
 1               5  


<210> 24
<211> 17
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH CDR2 of antibody 37M and 37C

<400> 24
Arg Ile Tyr Pro Gly Ser Gly Asn Thr Tyr Tyr Asn Glu Lys Phe Lys
 1               5                  10                  15      
Gly
    


<210> 25
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH CDR3 of antibody 37M and 37C

<400> 25
Gly Val Tyr Tyr Phe Asp Tyr
 1               5          


<210> 26
<211> 23
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL FR1 of antibody 37M and 37C

<400> 26
Asp Ile Val Met Thr Gln Ser Gln Lys Phe Met Ser Thr Ser Val Gly
 1               5                  10                  15      
Asp Arg Val Ser Val Thr Cys
            20              


<210> 27
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL FR2 of antibody 37M and 37C

<400> 27
Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Ala Leu Ile Tyr
 1               5                  10                  15  


<210> 28
<211> 32
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL FR3 of antibody 37M and 37C

<400> 28
Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr
 1               5                  10                  15      
Leu Thr Ile Ser Asn Val Gln Ser Glu Asp Leu Ala Asp Tyr Phe Cys
            20                  25                  30          


<210> 29
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL FR4 of antibody 37M and 37C

<400> 29
Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg
 1               5                  10       


<210> 30
<211> 30
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH FR1 of antibody 37M 

<400> 30
Gln Val Gln Leu Lys Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala
 1               5                  10                  15      
Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr
            20                  25                  30  


<210> 31
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH FR2 of antibody 37M

<400> 31
Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Ala
 1               5                  10                  


<210> 32
<211> 32
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH FR3 of antibody 37M

<400> 32
Lys Ala Thr Leu Thr Ala Glu Lys Ser Ser Ser Thr Ala Tyr Met Gln
 1               5                  10                  15      
Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg
            20                  25                  30          


<210> 33
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH FR4 of antibody 37M

<400> 33
Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser
 1               5                  10      


<210> 34

<400> 34
000                                                               3

<210> 35

<400> 35
000                                                               3

<210> 36

<400> 36
000                                                               3

<210> 37

<400> 37
000                                                               3

<210> 38
<211> 30
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH FR1 of antibody 37C

<400> 38
Gln Val Gln Leu Lys Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala
 1               5                  10                  15      
Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr
            20                  25                  30  


<210> 39
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH FR2 of antibody 37C

<400> 39
Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Ala
 1               5                  10                  


<210> 40
<211> 32
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH FR3 of antibody 37C

<400> 40
Lys Ala Thr Leu Thr Ala Glu Lys Ser Ser Ser Thr Ala Tyr Met Gln
 1               5                  10                  15      
Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg
            20                  25                  30          


<210> 41
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH FR4 of antibody 37C

<400> 41
Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ala
 1               5                  10      


<210> 42

<400> 42
000                                                               3

<210> 43
<211> 19
<212> PRT
<213> Artificial Sequence

<220> 
<223> signal peptide of antibody 37C or 37M light chain

<400> 43
Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly
 1               5                  10                  15      
Val His Ser
            


<210> 44
<211> 19
<212> PRT
<213> Artificial Sequence

<220> 
<223> signal peptide of antibody 37C or 37M heavy chain

<400> 44
Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly
 1               5                  10                  15      
Val His Ser
            


<210> 45
<211> 107
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL chain region of Ab1

<400> 45
Asp Ile Gln Met Thr Gln Ser Pro Thr Ser Leu Ser Ala Phe Val Gly
 1               5                  10                  15      
Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Asp Ile Gly Asn Tyr
            20                  25                  30          
Leu Asn Trp Tyr Gln Gln Lys Ser Gly Glu Pro Pro Lys Leu Leu Val
        35                  40                  45              
Tyr Asp Ala Ser Phe Leu Lys Lys Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Gln Tyr Phe Leu Thr Ile Tyr Ser Leu Gln Pro
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Phe Cys Gln His Ser Asp Asn Leu Ser Val
                85                  90                  95      
Thr Phe Gly Gly Gly Thr Lys Val Glu Val Lys
            100                 105         


<210> 46
<211> 122
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH chain region of Ab1 or Ab21

<400> 46
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
 1               5                  10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr
            20                  25                  30          
Leu Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
        35                  40                  45              
Ser Ser Ile Val Pro Ser Gly Gly Phe Thr His Tyr Ala Asp Ser Val
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95      
Ala Arg Leu Gln Thr Gly Ser Trp Arg Val His Ala Phe Asp Ile Trp
            100                 105                 110         
Gly Gln Gly Thr Met Val Thr Val Ser Ser
        115                 120         


<210> 47
<211> 107
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL chain region of Ab21

<400> 47
Asp Ile Gln Met Thr Gln Ser Pro Thr Ser Leu Ser Ala Phe Val Gly
 1               5                  10                  15      
Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Asp Ile Gly Asn Tyr
            20                  25                  30          
Leu Asn Trp Tyr Gln Gln Lys Ser Gly Glu Pro Pro Lys Leu Leu Val
        35                  40                  45              
Tyr Asp Ala Ser Phe Leu Lys Lys Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Gln Tyr Phe Leu Thr Ile Tyr Ser Leu Gln Pro
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Phe Cys Gln His Ser Asp Ser Leu Ser Val
                85                  90                  95      
Thr Phe Gly Gly Gly Thr Lys Val Glu Val Lys
            100                 105         


<210> 48
<211> 17
<212> PRT
<213> Artificial Sequence

<220> 
<223> a KIT epitope 

<400> 48
Ser Glu Leu His Leu Thr Arg Leu Lys Gly Thr Glu Gly Gly Thr Tyr
 1               5                  10                  15      
Thr
    


<210> 49
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> a KIT epitope

<400> 49
Leu Thr Arg Leu Lys Gly Thr Glu Gly Gly
 1               5                  10  

