                         SEQUENCE LISTING

<110>  GENTICEL
 
<120>  CYAA-CARRIED POLYPEPTIDE(S) AND USE TO INDUCE BOTH THERAPEUTIC 
       AND PROPHYLACTIC IMMUNE RESPONSES.

<130>  B09250A - AD/LV/KN

<140>  PCT/EP XXXXXX
<141>  2012-01-24

<150>  EP 11305069.4
<151>  2011-01-24

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  1706
<212>  PRT
<213>  Bordetella pertussis / CyaA

<400>  1

Met Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp Arg Glu 
1               5                   10                  15      


Ser Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val Ala Lys 
            20                  25                  30          


Glu Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His Ser Thr 
        35                  40                  45              


Ser Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val His Ala 
    50                  55                  60                  


Lys Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val Asn Pro 
65                  70                  75                  80  


Asn Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala Arg Ala 
                85                  90                  95      


Asp Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala Val Asp 
            100                 105                 110         


Leu Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala Gly Leu 
        115                 120                 125             


Val Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala Gly Tyr 
    130                 135                 140                 


Glu Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg Tyr Ala 
145                 150                 155                 160 


Val Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val Lys Val 
                165                 170                 175     


Ile Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Ile Asp Met Phe 
            180                 185                 190         


Ala Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg Ser Ser 
        195                 200                 205             


Val Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr Arg Arg 
    210                 215                 220                 


Ala Ala Ser Glu Ala Thr Gly Gly Leu Asp Arg Glu Arg Ile Asp Leu 
225                 230                 235                 240 


Leu Trp Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly Thr Glu 
                245                 250                 255     


Ala Arg Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly Val Ile 
            260                 265                 270         


Thr Asp Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg Ala His 
        275                 280                 285             


Ala Val Gly Ala Gln Asp Val Val Gln His Gly Thr Glu Gln Asn Asn 
    290                 295                 300                 


Pro Phe Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala Thr Gly 
305                 310                 315                 320 


Glu Ser Gln Met Leu Thr Arg Gly Gln Leu Lys Glu Tyr Ile Gly Gln 
                325                 330                 335     


Gln Arg Gly Glu Gly Tyr Val Phe Tyr Glu Asn Arg Ala Tyr Gly Val 
            340                 345                 350         


Ala Gly Lys Ser Leu Phe Asp Asp Gly Leu Gly Ala Ala Pro Gly Val 
        355                 360                 365             


Pro Ser Gly Arg Ser Lys Phe Ser Pro Asp Val Leu Glu Thr Val Pro 
    370                 375                 380                 


Ala Ser Pro Gly Leu Arg Arg Pro Ser Leu Gly Ala Val Glu Arg Gln 
385                 390                 395                 400 


Asp Ser Gly Tyr Asp Ser Leu Asp Gly Val Gly Ser Arg Ser Phe Ser 
                405                 410                 415     


Leu Gly Glu Val Ser Asp Met Ala Ala Val Glu Ala Ala Glu Leu Glu 
            420                 425                 430         


Met Thr Arg Gln Val Leu His Ala Gly Ala Arg Gln Asp Asp Ala Glu 
        435                 440                 445             


Pro Gly Val Ser Gly Ala Ser Ala His Trp Gly Gln Arg Ala Leu Gln 
    450                 455                 460                 


Gly Ala Gln Ala Val Ala Ala Ala Gln Arg Leu Val His Ala Ile Ala 
465                 470                 475                 480 


Leu Met Thr Gln Phe Gly Arg Ala Gly Ser Thr Asn Thr Pro Gln Glu 
                485                 490                 495     


Ala Ala Ser Leu Ser Ala Ala Val Phe Gly Leu Gly Glu Ala Ser Ser 
            500                 505                 510         


Ala Val Ala Glu Thr Val Ser Gly Phe Phe Arg Gly Ser Ser Arg Trp 
        515                 520                 525             


Ala Gly Gly Phe Gly Val Ala Gly Gly Ala Met Ala Leu Gly Gly Gly 
    530                 535                 540                 


Ile Ala Ala Ala Val Gly Ala Gly Met Ser Leu Thr Asp Asp Ala Pro 
545                 550                 555                 560 


Ala Gly Gln Lys Ala Ala Ala Gly Ala Glu Ile Ala Leu Gln Leu Thr 
                565                 570                 575     


Gly Gly Thr Val Glu Leu Ala Ser Ser Ile Ala Leu Ala Leu Ala Ala 
            580                 585                 590         


Ala Arg Gly Val Thr Ser Gly Leu Gln Val Ala Gly Ala Ser Ala Gly 
        595                 600                 605             


Ala Ala Ala Gly Ala Leu Ala Ala Ala Leu Ser Pro Met Glu Ile Tyr 
    610                 615                 620                 


Gly Leu Val Gln Gln Ser His Tyr Ala Asp Gln Leu Asp Lys Leu Ala 
625                 630                 635                 640 


Gln Glu Ser Ser Ala Tyr Gly Tyr Glu Gly Asp Ala Leu Leu Ala Gln 
                645                 650                 655     


Leu Tyr Arg Asp Lys Thr Ala Ala Glu Gly Ala Val Ala Gly Val Ser 
            660                 665                 670         


Ala Val Leu Ser Thr Val Gly Ala Ala Val Ser Ile Ala Ala Ala Ala 
        675                 680                 685             


Ser Val Val Gly Ala Pro Val Ala Val Val Thr Ser Leu Leu Thr Gly 
    690                 695                 700                 


Ala Leu Asn Gly Ile Leu Arg Gly Val Gln Gln Pro Ile Ile Glu Lys 
705                 710                 715                 720 


Leu Ala Asn Asp Tyr Ala Arg Lys Ile Asp Glu Leu Gly Gly Pro Gln 
                725                 730                 735     


Ala Tyr Phe Glu Lys Asn Leu Gln Ala Arg His Glu Gln Leu Ala Asn 
            740                 745                 750         


Ser Asp Gly Leu Arg Lys Met Leu Ala Asp Leu Gln Ala Gly Trp Asn 
        755                 760                 765             


Ala Ser Ser Val Ile Gly Val Gln Thr Thr Glu Ile Ser Lys Ser Ala 
    770                 775                 780                 


Leu Glu Leu Ala Ala Ile Thr Gly Asn Ala Asp Asn Leu Lys Ser Val 
785                 790                 795                 800 


Asp Val Phe Val Asp Arg Phe Val Gln Gly Glu Arg Val Ala Gly Gln 
                805                 810                 815     


Pro Val Val Leu Asp Val Ala Ala Gly Gly Ile Asp Ile Ala Ser Arg 
            820                 825                 830         


Lys Gly Glu Arg Pro Ala Leu Thr Phe Ile Thr Pro Leu Ala Ala Pro 
        835                 840                 845             


Gly Glu Glu Gln Arg Arg Arg Thr Lys Thr Gly Lys Ser Glu Phe Thr 
    850                 855                 860                 


Thr Phe Val Glu Ile Val Gly Lys Gln Asp Arg Trp Arg Ile Arg Asp 
865                 870                 875                 880 


Gly Ala Ala Asp Thr Thr Ile Asp Leu Ala Lys Val Val Ser Gln Leu 
                885                 890                 895     


Val Asp Ala Asn Gly Val Leu Lys His Ser Ile Lys Leu Asp Val Ile 
            900                 905                 910         


Gly Gly Asp Gly Asp Asp Val Val Leu Ala Asn Ala Ser Arg Ile His 
        915                 920                 925             


Tyr Asp Gly Gly Ala Gly Thr Asn Thr Val Ser Tyr Ala Ala Leu Gly 
    930                 935                 940                 


Arg Gln Asp Ser Ile Thr Val Ser Ala Asp Gly Glu Arg Phe Asn Val 
945                 950                 955                 960 


Arg Lys Gln Leu Asn Asn Ala Asn Val Tyr Arg Glu Gly Val Ala Thr 
                965                 970                 975     


Gln Thr Thr Ala Tyr Gly Lys Arg Thr Glu Asn Val Gln Tyr Arg His 
            980                 985                 990         


Val Glu Leu Ala Arg Val Gly Gln  Leu Val Glu Val Asp  Thr Leu Glu 
        995                 1000                 1005             


His Val  Gln His Ile Ile Gly  Gly Ala Gly Asn Asp  Ser Ile Thr 
    1010                 1015                 1020             


Gly Asn  Ala His Asp Asn Phe  Leu Ala Gly Gly Ser  Gly Asp Asp 
    1025                 1030                 1035             


Arg Leu  Asp Gly Gly Ala Gly  Asn Asp Thr Leu Val  Gly Gly Glu 
    1040                 1045                 1050             


Gly Gln  Asn Thr Val Ile Gly  Gly Ala Gly Asp Asp  Val Phe Leu 
    1055                 1060                 1065             


Gln Asp  Leu Gly Val Trp Ser  Asn Gln Leu Asp Gly  Gly Ala Gly 
    1070                 1075                 1080             


Val Asp  Thr Val Lys Tyr Asn  Val His Gln Pro Ser  Glu Glu Arg 
    1085                 1090                 1095             


Leu Glu  Arg Met Gly Asp Thr  Gly Ile His Ala Asp  Leu Gln Lys 
    1100                 1105                 1110             


Gly Thr  Val Glu Lys Trp Pro  Ala Leu Asn Leu Phe  Ser Val Asp 
    1115                 1120                 1125             


His Val  Lys Asn Ile Glu Asn  Leu His Gly Ser Arg  Leu Asn Asp 
    1130                 1135                 1140             


Arg Ile  Ala Gly Asp Asp Gln  Asp Asn Glu Leu Trp  Gly His Asp 
    1145                 1150                 1155             


Gly Asn  Asp Thr Ile Arg Gly  Arg Gly Gly Asp Asp  Ile Leu Arg 
    1160                 1165                 1170             


Gly Gly  Leu Gly Leu Asp Thr  Leu Tyr Gly Glu Asp  Gly Asn Asp 
    1175                 1180                 1185             


Ile Phe  Leu Gln Asp Asp Glu  Thr Val Ser Asp Asp  Ile Asp Gly 
    1190                 1195                 1200             


Gly Ala  Gly Leu Asp Thr Val  Asp Tyr Ser Ala Met  Ile His Pro 
    1205                 1210                 1215             


Gly Arg  Ile Val Ala Pro His  Glu Tyr Gly Phe Gly  Ile Glu Ala 
    1220                 1225                 1230             


Asp Leu  Ser Arg Glu Trp Val  Arg Lys Ala Ser Ala  Leu Gly Val 
    1235                 1240                 1245             


Asp Tyr  Tyr Asp Asn Val Arg  Asn Val Glu Asn Val  Ile Gly Thr 
    1250                 1255                 1260             


Ser Met  Lys Asp Val Leu Ile  Gly Asp Ala Gln Ala  Asn Thr Leu 
    1265                 1270                 1275             


Met Gly  Gln Gly Gly Asp Asp  Thr Val Arg Gly Gly  Asp Gly Asp 
    1280                 1285                 1290             


Asp Leu  Leu Phe Gly Gly Asp  Gly Asn Asp Met Leu  Tyr Gly Asp 
    1295                 1300                 1305             


Ala Gly  Asn Asp Thr Leu Tyr  Gly Gly Leu Gly Asp  Asp Thr Leu 
    1310                 1315                 1320             


Glu Gly  Gly Ala Gly Asn Asp  Trp Phe Gly Gln Thr  Gln Ala Arg 
    1325                 1330                 1335             


Glu His  Asp Val Leu Arg Gly  Gly Asp Gly Val Asp  Thr Val Asp 
    1340                 1345                 1350             


Tyr Ser  Gln Thr Gly Ala His  Ala Gly Ile Ala Ala  Gly Arg Ile 
    1355                 1360                 1365             


Gly Leu  Gly Ile Leu Ala Asp  Leu Gly Ala Gly Arg  Val Asp Lys 
    1370                 1375                 1380             


Leu Gly  Glu Ala Gly Ser Ser  Ala Tyr Asp Thr Val  Ser Gly Ile 
    1385                 1390                 1395             


Glu Asn  Val Val Gly Thr Glu  Leu Ala Asp Arg Ile  Thr Gly Asp 
    1400                 1405                 1410             


Ala Gln  Ala Asn Val Leu Arg  Gly Ala Gly Gly Ala  Asp Val Leu 
    1415                 1420                 1425             


Ala Gly  Gly Glu Gly Asp Asp  Val Leu Leu Gly Gly  Asp Gly Asp 
    1430                 1435                 1440             


Asp Gln  Leu Ser Gly Asp Ala  Gly Arg Asp Arg Leu  Tyr Gly Glu 
    1445                 1450                 1455             


Ala Gly  Asp Asp Trp Phe Phe  Gln Asp Ala Ala Asn  Ala Gly Asn 
    1460                 1465                 1470             


Leu Leu  Asp Gly Gly Asp Gly  Arg Asp Thr Val Asp  Phe Ser Gly 
    1475                 1480                 1485             


Pro Gly  Arg Gly Leu Asp Ala  Gly Ala Lys Gly Val  Phe Leu Ser 
    1490                 1495                 1500             


Leu Gly  Lys Gly Phe Ala Ser  Leu Met Asp Glu Pro  Glu Thr Ser 
    1505                 1510                 1515             


Asn Val  Leu Arg Asn Ile Glu  Asn Ala Val Gly Ser  Ala Arg Asp 
    1520                 1525                 1530             


Asp Val  Leu Ile Gly Asp Ala  Gly Ala Asn Val Leu  Asn Gly Leu 
    1535                 1540                 1545             


Ala Gly  Asn Asp Val Leu Ser  Gly Gly Ala Gly Asp  Asp Val Leu 
    1550                 1555                 1560             


Leu Gly  Asp Glu Gly Ser Asp  Leu Leu Ser Gly Asp  Ala Gly Asn 
    1565                 1570                 1575             


Asp Asp  Leu Phe Gly Gly Gln  Gly Asp Asp Thr Tyr  Leu Phe Gly 
    1580                 1585                 1590             


Val Gly  Tyr Gly His Asp Thr  Ile Tyr Glu Ser Gly  Gly Gly His 
    1595                 1600                 1605             


Asp Thr  Ile Arg Ile Asn Ala  Gly Ala Asp Gln Leu  Trp Phe Ala 
    1610                 1615                 1620             


Arg Gln  Gly Asn Asp Leu Glu  Ile Arg Ile Leu Gly  Thr Asp Asp 
    1625                 1630                 1635             


Ala Leu  Thr Val His Asp Trp  Tyr Arg Asp Ala Asp  His Arg Val 
    1640                 1645                 1650             


Glu Ile  Ile His Ala Ala Asn  Gln Ala Val Asp Gln  Ala Gly Ile 
    1655                 1660                 1665             


Glu Lys  Leu Val Glu Ala Met  Ala Gln Tyr Pro Asp  Pro Gly Ala 
    1670                 1675                 1680             


Ala Ala  Ala Ala Pro Pro Ala  Ala Arg Val Pro Asp  Thr Leu Met 
    1685                 1690                 1695             


Gln Ser  Leu Ala Val Asn Trp  Arg 
    1700                 1705     


<210>  2
<211>  1740
<212>  PRT
<213>  Bordetella parapertussis / CyaA

<400>  2

Met Leu Asp Val Trp Phe Leu Gln Lys Asp Glu Val Leu Ser Ala Thr 
1               5                   10                  15      


His Arg Leu Arg Arg Cys Glu Ser Val Gln Ser Thr Thr Tyr Arg Gln 
            20                  25                  30          


Ile His Met Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp 
        35                  40                  45              


Arg Glu Ser Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val 
    50                  55                  60                  


Ala Lys Glu Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His 
65                  70                  75                  80  


Ser Thr Ser Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val 
                85                  90                  95      


His Ala Lys Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val 
            100                 105                 110         


Asn Pro Asn Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala 
        115                 120                 125             


Arg Ala Asp Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala 
    130                 135                 140                 


Val Asp Leu Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala 
145                 150                 155                 160 


Gly Leu Val Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala 
                165                 170                 175     


Gly Tyr Glu Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg 
            180                 185                 190         


Tyr Ala Val Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val 
        195                 200                 205             


Lys Val Ile Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Ile Asp 
    210                 215                 220                 


Met Phe Ala Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg 
225                 230                 235                 240 


Ser Ser Val Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr 
                245                 250                 255     


Arg Arg Ala Ala Ser Glu Ala Thr Gly Gly Leu Asp Arg Glu Arg Ile 
            260                 265                 270         


Asp Leu Leu Trp Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly 
        275                 280                 285             


Thr Glu Ala Arg Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly 
    290                 295                 300                 


Val Ile Thr Asp Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg 
305                 310                 315                 320 


Ala His Ala Val Gly Ala Gln Asp Val Val Gln His Gly Thr Glu Gln 
                325                 330                 335     


Asn Asn Pro Phe Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala 
            340                 345                 350         


Thr Gly Glu Ser Gln Met Leu Thr Arg Gly Gln Leu Lys Glu Tyr Ile 
        355                 360                 365             


Gly Gln Gln Arg Gly Glu Gly Tyr Val Phe Tyr Glu Asn Arg Ala Tyr 
    370                 375                 380                 


Gly Val Ala Gly Lys Ser Leu Phe Asp Asp Gly Leu Gly Ala Ala Pro 
385                 390                 395                 400 


Gly Val Pro Gly Gly Arg Ser Lys Ser Ser Pro Asp Val Leu Glu Thr 
                405                 410                 415     


Val Pro Ala Ser Pro Gly Leu Arg Arg Pro Ser Leu Gly Ala Val Glu 
            420                 425                 430         


Arg Gln Asp Ser Gly Tyr Asp Ser Leu Asp Gly Val Gly Ser Arg Ser 
        435                 440                 445             


Phe Ser Leu Gly Glu Val Ser Asp Met Ala Ala Val Glu Ala Ala Glu 
    450                 455                 460                 


Leu Glu Met Thr Arg Gln Val Leu His Ala Gly Ala Arg Gln Asp Asp 
465                 470                 475                 480 


Ala Glu Pro Gly Val Ser Gly Ala Ser Ala His Trp Gly Gln Arg Ala 
                485                 490                 495     


Leu Gln Gly Ala Gln Ala Val Ala Ala Ala Gln Arg Leu Val His Ala 
            500                 505                 510         


Ile Ala Leu Met Thr Gln Phe Gly Arg Ala Gly Ser Thr Asn Thr Pro 
        515                 520                 525             


Gln Glu Ala Ala Ser Leu Ser Ala Ala Val Phe Gly Leu Gly Glu Ala 
    530                 535                 540                 


Ser Ser Ala Val Ala Glu Thr Val Ser Gly Phe Phe Arg Gly Ser Ser 
545                 550                 555                 560 


Arg Trp Ala Gly Gly Phe Gly Val Ala Gly Gly Ala Met Ala Leu Gly 
                565                 570                 575     


Gly Gly Ile Ala Ala Ala Val Gly Ala Gly Met Ser Leu Thr Asp Asp 
            580                 585                 590         


Ala Pro Ala Gly Gln Lys Ala Ala Val Gly Ala Glu Ile Ala Leu Gln 
        595                 600                 605             


Leu Thr Gly Gly Thr Val Glu Leu Ala Ser Ser Ile Ala Leu Ala Leu 
    610                 615                 620                 


Ala Ala Ala Arg Gly Val Thr Ser Gly Leu Gln Val Ala Gly Ala Ser 
625                 630                 635                 640 


Ala Gly Ala Ala Ala Gly Ala Leu Ala Ala Ala Leu Ser Pro Met Glu 
                645                 650                 655     


Ile Tyr Gly Leu Val Gln Gln Ser His Tyr Ala Asp Gln Leu Asp Lys 
            660                 665                 670         


Leu Ala Gln Glu Ser Ser Ala Tyr Gly Tyr Glu Gly Asp Ala Leu Leu 
        675                 680                 685             


Ala Gln Leu Tyr Arg Asp Lys Thr Ala Ala Glu Gly Ala Val Ala Gly 
    690                 695                 700                 


Val Ser Ala Val Leu Ser Thr Val Gly Ala Ala Val Ser Ile Ala Ala 
705                 710                 715                 720 


Ala Ala Ser Val Val Gly Ala Pro Val Ala Val Val Thr Ser Leu Leu 
                725                 730                 735     


Thr Gly Ala Leu Asn Gly Ile Leu Arg Gly Val Gln Gln Pro Ile Ile 
            740                 745                 750         


Glu Lys Leu Ala Asn Asp Tyr Ala Arg Lys Ile Asp Glu Leu Gly Gly 
        755                 760                 765             


Pro Gln Ala Tyr Phe Glu Lys Asn Leu Gln Ala Arg His Glu Gln Leu 
    770                 775                 780                 


Ala Asn Ser Asp Gly Leu Arg Lys Met Leu Ala Asp Leu Gln Ala Gly 
785                 790                 795                 800 


Trp Asn Ala Ser Ser Val Ile Gly Val Gln Thr Thr Glu Ile Ser Lys 
                805                 810                 815     


Ser Ala Leu Glu Leu Ala Ala Ile Thr Gly Asn Ala Asp Asn Leu Lys 
            820                 825                 830         


Ser Ala Asp Val Phe Val Asp Arg Phe Ile Gln Gly Glu Arg Val Ala 
        835                 840                 845             


Gly Gln Pro Val Val Leu Asp Val Ala Ala Gly Gly Ile Asp Ile Ala 
    850                 855                 860                 


Ser Arg Lys Gly Glu Arg Pro Ala Leu Thr Phe Ile Thr Pro Leu Ala 
865                 870                 875                 880 


Ala Pro Gly Glu Glu Gln Arg Arg Arg Thr Lys Thr Gly Lys Ser Glu 
                885                 890                 895     


Phe Thr Thr Phe Val Glu Ile Val Gly Lys Gln Asp Arg Trp Arg Ile 
            900                 905                 910         


Arg Asp Gly Ala Ala Asp Thr Thr Ile Asp Leu Ala Lys Val Val Ser 
        915                 920                 925             


Gln Leu Val Asp Ala Asn Gly Val Leu Lys His Ser Ile Lys Leu Glu 
    930                 935                 940                 


Val Ile Gly Gly Asp Gly Asp Asp Val Val Leu Ala Asn Ala Ser Arg 
945                 950                 955                 960 


Ile His Tyr Asp Gly Gly Ala Gly Thr Asn Thr Val Ser Tyr Ala Ala 
                965                 970                 975     


Leu Gly Arg Gln Asp Ser Ile Thr Val Ser Ala Asp Gly Glu Arg Phe 
            980                 985                 990         


Asn Val Arg Lys Gln Leu Asn Asn  Ala Asn Val Tyr Arg  Glu Gly Val 
        995                 1000                 1005             


Ala Thr  Gln Lys Thr Ala Tyr  Gly Lys Arg Thr Glu  Asn Val Gln 
    1010                 1015                 1020             


Tyr Arg  His Val Glu Leu Ala  Arg Val Gly Gln Leu  Val Glu Val 
    1025                 1030                 1035             


Asp Thr  Leu Glu His Val Gln  His Ile Ile Gly Gly  Ala Gly Asn 
    1040                 1045                 1050             


Asp Ser  Ile Thr Gly Asn Ala  His Asp Asn Phe Leu  Ala Gly Gly 
    1055                 1060                 1065             


Ala Gly  Asp Asp Arg Leu Asp  Gly Gly Ala Gly Asn  Asp Thr Leu 
    1070                 1075                 1080             


Val Gly  Gly Glu Gly His Asn  Thr Val Val Gly Gly  Ala Gly Asp 
    1085                 1090                 1095             


Asp Val  Phe Leu Gln Asp Leu  Gly Val Trp Ser Asn  Gln Leu Asp 
    1100                 1105                 1110             


Gly Gly  Ala Gly Val Asp Thr  Val Lys Tyr Asn Val  His Gln Pro 
    1115                 1120                 1125             


Ser Glu  Glu Arg Leu Glu Arg  Met Gly Asp Thr Gly  Ile His Ala 
    1130                 1135                 1140             


Asp Leu  Gln Lys Gly Thr Val  Glu Lys Trp Pro Ala  Leu Asn Leu 
    1145                 1150                 1155             


Phe Ser  Val Asp His Val Lys  Asn Ile Glu Asn Leu  His Gly Ser 
    1160                 1165                 1170             


Ser Leu  Asn Asp Ser Ile Ala  Gly Asp Asp Arg Asp  Asn Glu Leu 
    1175                 1180                 1185             


Trp Gly  Asp Asp Gly Asn Asp  Thr Ile His Gly Arg  Gly Gly Asp 
    1190                 1195                 1200             


Asp Ile  Leu Arg Gly Gly Leu  Gly Leu Asp Thr Leu  Tyr Gly Glu 
    1205                 1210                 1215             


Asp Gly  Asn Asp Ile Phe Leu  Gln Asp Asp Glu Thr  Val Ser Asp 
    1220                 1225                 1230             


Asp Ile  Asp Gly Gly Ala Gly  Leu Asp Thr Val Asp  Tyr Ser Ala 
    1235                 1240                 1245             


Met Ile  His Ala Gly Lys Ile  Val Ala Pro His Glu  Tyr Gly Phe 
    1250                 1255                 1260             


Gly Ile  Glu Ala Asp Leu Ser  Glu Gly Trp Val Arg  Lys Ala Ala 
    1265                 1270                 1275             


Arg Arg  Gly Met Gly Tyr Tyr  Asp Ser Val Arg Ser  Val Glu Asn 
    1280                 1285                 1290             


Val Ile  Gly Thr Ser Met Lys  Asp Val Leu Ile Gly  Asp Ala Gln 
    1295                 1300                 1305             


Ala Asn  Thr Leu Met Gly Gln  Gly Gly Asp Asp Thr  Val Arg Gly 
    1310                 1315                 1320             


Gly Asp  Gly Asp Asp Leu Leu  Phe Gly Gly Asp Gly  Asn Asp Met 
    1325                 1330                 1335             


Leu Tyr  Gly Asp Ala Gly Asn  Asp Thr Leu Tyr Gly  Gly Leu Gly 
    1340                 1345                 1350             


Asp Asp  Thr Leu Glu Gly Gly  Ala Gly Asn Asp Trp  Phe Gly Gln 
    1355                 1360                 1365             


Thr Pro  Ala Arg Glu His Asp  Val Leu Arg Gly Gly  Ala Gly Val 
    1370                 1375                 1380             


Asp Thr  Val Asp Tyr Ser Gln  Ala Gly Ala His Ala  Gly Val Ala 
    1385                 1390                 1395             


Thr Gly  Arg Ile Gly Leu Gly  Ile Leu Ala Asp Leu  Gly Ala Gly 
    1400                 1405                 1410             


Arg Val  Asp Lys Leu Gly Glu  Ala Gly Ser Ser Ala  Tyr Asp Thr 
    1415                 1420                 1425             


Val Ser  Gly Ile Glu Asn Val  Val Gly Thr Glu Leu  Ala Asp Arg 
    1430                 1435                 1440             


Ile Thr  Gly Asp Ala Gln Ala  Asn Val Leu Arg Gly  Ala Gly Gly 
    1445                 1450                 1455             


Ala Asp  Val Leu Ala Gly Gly  Glu Gly Asp Asp Val  Leu Leu Gly 
    1460                 1465                 1470             


Gly Glu  Gly Asp Asp Gln Leu  Ser Gly Asp Ala Gly  Arg Asp Arg 
    1475                 1480                 1485             


Leu Tyr  Gly Glu Ala Gly Asp  Asp Trp Phe Phe Gln  Asp Ala Ala 
    1490                 1495                 1500             


Asn Ala  Gly Asn Leu Leu Asp  Gly Gly Asp Gly Asn  Asp Thr Val 
    1505                 1510                 1515             


Asp Phe  Ser Gly Pro Gly Arg  Gly Leu Asp Ala Gly  Ala Lys Gly 
    1520                 1525                 1530             


Val Phe  Leu Ser Leu Gly Lys  Gly Phe Ala Ser Leu  Met Asp Glu 
    1535                 1540                 1545             


Pro Glu  Thr Ser Asn Val Leu  Arg His Ile Glu Asn  Ala Val Gly 
    1550                 1555                 1560             


Ser Val  Arg Asp Asp Val Leu  Ile Gly Asp Ala Gly  Ala Asn Val 
    1565                 1570                 1575             


Leu Asn  Gly Leu Ala Gly Asn  Asp Val Leu Ser Gly  Gly Ala Gly 
    1580                 1585                 1590             


Asp Asp  Val Leu Leu Gly Asp  Glu Gly Ser Asp Leu  Leu Ser Gly 
    1595                 1600                 1605             


Asp Ala  Gly Asn Asp Asp Leu  Phe Gly Gly Gln Gly  Asp Asp Thr 
    1610                 1615                 1620             


Tyr Leu  Phe Gly Ala Gly Tyr  Gly His Asp Thr Ile  Tyr Glu Ser 
    1625                 1630                 1635             


Gly Gly  Gly His Asp Thr Ile  Arg Ile Asn Ala Gly  Ala Asp Gln 
    1640                 1645                 1650             


Leu Trp  Phe Ala Arg Gln Gly  Asn Asp Leu Glu Ile  Arg Ile Leu 
    1655                 1660                 1665             


Gly Thr  Asp Asp Ala Leu Thr  Val His Asp Trp Tyr  Arg Asp Ala 
    1670                 1675                 1680             


Asp His  Arg Val Glu Ala Ile  His Ala Ala Asn Gln  Ala Ile Asp 
    1685                 1690                 1695             


Pro Ala  Gly Ile Glu Lys Leu  Val Glu Ala Met Ala  Gln Tyr Pro 
    1700                 1705                 1710             


Asp Pro  Gly Ala Ala Ala Ala  Ala Pro Pro Ala Ala  Arg Val Pro 
    1715                 1720                 1725             


Asp Thr  Leu Met Gln Ser Leu  Ala Val Asn Trp Arg  
    1730                 1735                 1740 


<210>  3
<211>  1740
<212>  PRT
<213>  Bordetella bronchiseptica / CyaA

<400>  3

Met Leu Asp Val Trp Phe Leu Gln Lys Asp Glu Val Leu Ser Ala Thr 
1               5                   10                  15      


His Arg Leu Arg Arg Cys Glu Ser Val Gln Ser Thr Thr Tyr Arg Gln 
            20                  25                  30          


Ile His Met Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp 
        35                  40                  45              


Arg Glu Ser Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val 
    50                  55                  60                  


Ala Lys Glu Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His 
65                  70                  75                  80  


Ser Thr Ser Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val 
                85                  90                  95      


His Ala Lys Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val 
            100                 105                 110         


Asn Pro Asn Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala 
        115                 120                 125             


Arg Ala Asp Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala 
    130                 135                 140                 


Val Asp Leu Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala 
145                 150                 155                 160 


Gly Leu Val Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala 
                165                 170                 175     


Gly Tyr Glu Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg 
            180                 185                 190         


Tyr Ala Val Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val 
        195                 200                 205             


Lys Val Ile Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Ile Asp 
    210                 215                 220                 


Met Phe Ala Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg 
225                 230                 235                 240 


Ser Ser Val Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr 
                245                 250                 255     


Arg Arg Ala Ala Ser Glu Ala Thr Gly Gly Leu Asp Arg Glu Arg Ile 
            260                 265                 270         


Asp Leu Leu Trp Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly 
        275                 280                 285             


Thr Glu Ala Arg Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly 
    290                 295                 300                 


Val Ile Thr Asp Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg 
305                 310                 315                 320 


Ala His Ala Val Gly Ala Gln Asp Val Val Gln His Gly Thr Glu Gln 
                325                 330                 335     


Asn Asn Pro Phe Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala 
            340                 345                 350         


Thr Gly Glu Ser Gln Met Leu Thr Arg Gly Gln Leu Lys Glu Tyr Ile 
        355                 360                 365             


Gly Gln Gln Arg Gly Glu Gly Tyr Val Phe Tyr Glu Asn Arg Ala Tyr 
    370                 375                 380                 


Gly Val Ala Gly Lys Ser Leu Phe Asp Asp Gly Leu Gly Ala Ala Pro 
385                 390                 395                 400 


Gly Val Pro Gly Gly Arg Ser Lys Ser Ser Pro Asp Val Leu Glu Thr 
                405                 410                 415     


Val Pro Ala Ser Pro Gly Leu Arg Arg Pro Ser Leu Gly Ala Val Glu 
            420                 425                 430         


Arg Gln Asp Ser Gly Tyr Asp Ser Leu Asp Gly Val Gly Ser Arg Ser 
        435                 440                 445             


Phe Ser Leu Gly Glu Val Ser Asp Met Ala Ala Val Glu Ala Ala Glu 
    450                 455                 460                 


Leu Glu Met Thr Arg Gln Val Leu His Ala Gly Ala Arg Gln Asp Asp 
465                 470                 475                 480 


Ala Glu Pro Gly Val Ser Gly Ala Ser Ala His Trp Gly Gln Arg Ala 
                485                 490                 495     


Leu Gln Gly Ala Gln Ala Val Ala Ala Ala Gln Arg Leu Val His Ala 
            500                 505                 510         


Ile Ala Leu Met Thr Gln Phe Gly Arg Ala Gly Ser Thr Asn Thr Pro 
        515                 520                 525             


Gln Glu Ala Ala Ser Leu Ser Ala Ala Val Phe Gly Leu Gly Glu Ala 
    530                 535                 540                 


Ser Ser Ala Val Ala Glu Thr Val Ser Gly Phe Phe Arg Gly Ser Ser 
545                 550                 555                 560 


Arg Trp Ala Gly Gly Phe Gly Val Ala Gly Gly Ala Met Ala Leu Gly 
                565                 570                 575     


Gly Gly Ile Ala Ala Ala Val Gly Ala Gly Met Ser Leu Thr Asp Asp 
            580                 585                 590         


Ala Pro Ala Gly Gln Lys Ala Ala Ala Gly Ala Glu Ile Ala Leu Gln 
        595                 600                 605             


Leu Thr Gly Gly Thr Val Glu Leu Ala Ser Ser Ile Ala Leu Ala Leu 
    610                 615                 620                 


Ala Ala Ala Arg Gly Val Thr Ser Gly Leu Gln Val Ala Gly Ala Ser 
625                 630                 635                 640 


Ala Gly Ala Ala Ala Gly Ala Leu Ala Ala Ala Leu Ser Pro Met Glu 
                645                 650                 655     


Ile Tyr Gly Leu Val Gln Gln Ser His Tyr Ala Asp Gln Leu Asp Lys 
            660                 665                 670         


Leu Ala Gln Glu Ser Ser Ala Tyr Gly Tyr Glu Gly Asp Ala Leu Leu 
        675                 680                 685             


Ala Gln Leu Tyr Arg Asp Lys Thr Ala Ala Glu Gly Ala Val Ala Gly 
    690                 695                 700                 


Val Ser Ala Val Leu Ser Thr Val Gly Ala Ala Val Ser Ile Ala Ala 
705                 710                 715                 720 


Ala Ala Ser Val Val Gly Ala Pro Val Ala Val Val Thr Ser Leu Leu 
                725                 730                 735     


Thr Gly Ala Leu Asn Gly Ile Leu Arg Gly Val Gln Gln Pro Ile Ile 
            740                 745                 750         


Glu Lys Leu Ala Asn Asp Tyr Ala Arg Lys Ile Asp Glu Leu Gly Gly 
        755                 760                 765             


Pro Gln Ala Tyr Phe Glu Lys Asn Leu Gln Ala Arg His Glu Gln Leu 
    770                 775                 780                 


Ala Asn Ser Asp Gly Leu Arg Lys Met Leu Ala Asp Leu Gln Ala Gly 
785                 790                 795                 800 


Trp Asn Ala Ser Ser Val Ile Gly Val Gln Thr Thr Glu Ile Ser Lys 
                805                 810                 815     


Ser Ala Leu Glu Leu Ala Ala Ile Thr Gly Asn Ala Asp Asn Leu Lys 
            820                 825                 830         


Ser Ala Asp Val Phe Val Asp Arg Phe Ile Gln Gly Glu Arg Val Ala 
        835                 840                 845             


Gly Gln Pro Val Val Leu Asp Val Ala Ala Gly Gly Ile Asp Ile Ala 
    850                 855                 860                 


Ser Arg Lys Gly Glu Arg Pro Ala Leu Thr Phe Ile Thr Pro Leu Ala 
865                 870                 875                 880 


Ala Pro Gly Glu Glu Gln Arg Arg Arg Thr Lys Thr Gly Lys Ser Glu 
                885                 890                 895     


Phe Thr Thr Phe Val Glu Ile Val Gly Lys Gln Asp Arg Trp Arg Ile 
            900                 905                 910         


Arg Asp Gly Ala Ala Asp Thr Thr Ile Asp Leu Ala Lys Val Val Ser 
        915                 920                 925             


Gln Leu Val Asp Ala Asn Gly Val Leu Lys His Ser Ile Lys Leu Glu 
    930                 935                 940                 


Val Ile Gly Gly Asp Gly Asp Asp Val Val Leu Ala Asn Ala Ser Arg 
945                 950                 955                 960 


Ile His Tyr Asp Gly Gly Ala Gly Thr Asn Thr Val Ser Tyr Ala Ala 
                965                 970                 975     


Leu Gly Arg Gln Asp Ser Ile Thr Val Ser Ala Asp Gly Glu Arg Phe 
            980                 985                 990         


Asn Val Arg Lys Gln Leu Asn Asn  Ala Asn Val Tyr Arg  Glu Gly Val 
        995                 1000                 1005             


Ala Thr  Gln Lys Thr Ala Tyr  Gly Lys Arg Thr Glu  Asn Val Gln 
    1010                 1015                 1020             


Tyr Arg  His Val Glu Leu Ala  Arg Val Gly Gln Leu  Val Glu Val 
    1025                 1030                 1035             


Asp Thr  Leu Glu His Val Gln  His Ile Ile Gly Gly  Ala Gly Asn 
    1040                 1045                 1050             


Asp Ser  Ile Thr Gly Asn Ala  His Asp Asn Phe Leu  Ala Gly Gly 
    1055                 1060                 1065             


Ala Gly  Asp Asp Arg Leu Asp  Gly Gly Ala Gly Asn  Asp Thr Leu 
    1070                 1075                 1080             


Val Gly  Gly Glu Gly His Asn  Thr Val Val Gly Gly  Ala Gly Asp 
    1085                 1090                 1095             


Asp Val  Phe Leu Gln Asp Leu  Gly Val Trp Ser Asn  Gln Leu Asp 
    1100                 1105                 1110             


Gly Gly  Ala Gly Val Asp Thr  Val Lys Tyr Asn Val  His Gln Pro 
    1115                 1120                 1125             


Ser Glu  Glu Arg Leu Glu Arg  Met Gly Asp Thr Gly  Ile His Ala 
    1130                 1135                 1140             


Asp Leu  Gln Lys Gly Thr Val  Glu Lys Trp Pro Ala  Leu Asn Leu 
    1145                 1150                 1155             


Phe Ser  Val Asp His Val Lys  Asn Ile Glu Asn Leu  His Gly Ser 
    1160                 1165                 1170             


Ser Leu  Asn Asp Ser Ile Ala  Gly Asp Asp Arg Asp  Asn Glu Leu 
    1175                 1180                 1185             


Trp Gly  Asp Asp Gly Asn Asp  Thr Ile His Gly Arg  Gly Gly Asp 
    1190                 1195                 1200             


Asp Ile  Leu Arg Gly Gly Leu  Gly Leu Asp Thr Leu  Tyr Gly Glu 
    1205                 1210                 1215             


Asp Gly  Asn Asp Ile Phe Leu  Gln Asp Asp Glu Thr  Val Ser Asp 
    1220                 1225                 1230             


Asp Ile  Asp Gly Gly Ala Gly  Leu Asp Thr Val Asp  Tyr Ser Ala 
    1235                 1240                 1245             


Met Ile  His Ala Gly Lys Ile  Val Ala Pro His Glu  Tyr Gly Phe 
    1250                 1255                 1260             


Gly Ile  Glu Ala Asp Leu Ser  Glu Gly Trp Val Arg  Lys Ala Ala 
    1265                 1270                 1275             


Arg Arg  Gly Met Asp Tyr Tyr  Asp Ser Val Arg Ser  Val Glu Asn 
    1280                 1285                 1290             


Val Ile  Gly Thr Ser Met Lys  Asp Val Leu Ile Gly  Asp Ala Gln 
    1295                 1300                 1305             


Ala Asn  Thr Leu Met Gly Gln  Gly Gly Asp Asp Thr  Val Arg Gly 
    1310                 1315                 1320             


Gly Asp  Gly Asp Asp Leu Leu  Phe Gly Gly Asp Gly  Asn Asp Met 
    1325                 1330                 1335             


Leu Tyr  Gly Asp Ala Gly Asn  Asp Thr Leu Tyr Gly  Gly Leu Gly 
    1340                 1345                 1350             


Asp Asp  Thr Leu Glu Gly Gly  Ala Gly Asn Asp Trp  Phe Gly Gln 
    1355                 1360                 1365             


Thr Pro  Ala Arg Glu His Asp  Val Leu Arg Gly Gly  Ala Gly Val 
    1370                 1375                 1380             


Asp Thr  Val Asp Tyr Ser Gln  Ala Gly Ala His Ala  Gly Val Ala 
    1385                 1390                 1395             


Thr Gly  Arg Ile Gly Leu Gly  Ile Leu Ala Asp Leu  Gly Ala Gly 
    1400                 1405                 1410             


Arg Val  Asp Lys Leu Gly Glu  Ala Gly Ser Ser Ala  Tyr Asp Thr 
    1415                 1420                 1425             


Val Ser  Gly Ile Glu Asn Val  Val Gly Thr Glu Leu  Ala Asp Arg 
    1430                 1435                 1440             


Ile Thr  Gly Asp Ala Gln Ala  Asn Val Leu Arg Gly  Ala Gly Gly 
    1445                 1450                 1455             


Ala Asp  Val Leu Ala Gly Gly  Glu Gly Asp Asp Val  Leu Leu Gly 
    1460                 1465                 1470             


Gly Asp  Gly Asp Asp Gln Leu  Ser Gly Asp Ala Gly  Arg Asp Arg 
    1475                 1480                 1485             


Leu Tyr  Gly Glu Ala Gly Asp  Asp Trp Phe Phe Gln  Asp Ala Ala 
    1490                 1495                 1500             


Asn Ala  Gly Asn Leu Leu Asp  Gly Gly Asp Gly Asn  Asp Thr Val 
    1505                 1510                 1515             


Asp Phe  Ser Gly Pro Gly Arg  Gly Leu Asp Ala Gly  Ala Lys Gly 
    1520                 1525                 1530             


Val Phe  Leu Ser Leu Gly Lys  Gly Phe Ala Ser Leu  Met Asp Glu 
    1535                 1540                 1545             


Pro Glu  Thr Ser Asn Val Leu  Arg His Ile Glu Asn  Ala Val Gly 
    1550                 1555                 1560             


Ser Val  Arg Asp Asp Val Leu  Ile Gly Asp Ala Gly  Ala Asn Val 
    1565                 1570                 1575             


Leu Asn  Gly Leu Ala Gly Asn  Asp Val Leu Ser Gly  Gly Ala Gly 
    1580                 1585                 1590             


Asp Asp  Val Leu Leu Gly Asp  Glu Gly Ser Asp Leu  Leu Ser Gly 
    1595                 1600                 1605             


Asp Ala  Gly Asn Asp Asp Leu  Phe Gly Gly Gln Gly  Asp Asp Thr 
    1610                 1615                 1620             


Tyr Leu  Phe Gly Ala Gly Tyr  Gly His Asp Thr Ile  Tyr Glu Ser 
    1625                 1630                 1635             


Gly Gly  Gly His Asp Thr Ile  Arg Ile Asn Ala Gly  Ala Asp Gln 
    1640                 1645                 1650             


Leu Trp  Phe Ala Arg Gln Gly  Asn Asp Leu Glu Ile  Arg Ile Leu 
    1655                 1660                 1665             


Gly Thr  Asp Asp Ala Leu Thr  Val His Asp Trp Tyr  Arg Asp Ala 
    1670                 1675                 1680             


Asp His  Arg Val Glu Ala Ile  His Ala Ala Asn Gln  Ala Ile Asp 
    1685                 1690                 1695             


Pro Ala  Gly Ile Glu Lys Leu  Val Glu Ala Met Ala  Gln Tyr Pro 
    1700                 1705                 1710             


Asp Pro  Gly Ala Ala Ala Ala  Ala Pro Pro Ala Ala  Arg Val Pro 
    1715                 1720                 1725             


Asp Thr  Leu Met Gln Ser Leu  Ala Val Asn Trp Arg  
    1730                 1735                 1740 


<210>  4
<211>  5121
<212>  DNA
<213>  Bordetella pertussis / cyaA gene

<400>  4
atgcagcaat cgcatcaggc tggttacgca aacgccgccg accgggagtc tggcatcccc       60

gcagccgtac tcgatggcat caaggccgtg gcgaaggaaa aaaacgccac attgatgttc      120

cgcctggtca acccccattc caccagcctg attgccgaag gggtggccac caaaggattg      180

ggcgtgcacg ccaagtcgtc cgattggggg ttgcaggcgg gctacattcc cgtcaacccg      240

aatctttcca aactgttcgg ccgtgcgccc gaggtgatcg cgcgggccga caacgacgtc      300

aacagcagcc tggcgcatgg ccataccgcg gtcgacctga cgctgtcgaa agagcggctt      360

gactatctgc ggcaagcggg cctggtcacc ggcatggccg atggcgtggt cgcgagcaac      420

cacgcaggct acgagcagtt cgagtttcgc gtgaaggaaa cctcggacgg gcgctatgcc      480

gtgcagtatc gccgcaaggg cggcgacgat ttcgaggcgg tcaaggtgat cggcaatgcc      540

gccggtattc cactgacggc ggatatcgac atgttcgcca ttatgccgca tctgtccaac      600

ttccgcgact cggcgcgcag ttcggtgacc agcggcgatt cggtgaccga ttacctggcg      660

cgcacgcggc gggccgccag cgaggccacg ggcggcctgg atcgcgaacg catcgacttg      720

ttgtggaaaa tcgctcgcgc cggcgcccgt tccgcagtgg gcaccgaggc gcgtcgccag      780

ttccgctacg acggcgacat gaatatcggc gtgatcaccg atttcgagct ggaagtgcgc      840

aatgcgctga acaggcgggc gcacgccgtc ggcgcgcagg acgtggtcca gcatggcact      900

gagcagaaca atcctttccc ggaggcagat gagaagattt tcgtcgtatc ggccaccggt      960

gaaagccaga tgctcacgcg cgggcaactg aaggaataca ttggccagca gcgcggcgag     1020

ggctatgtct tctacgagaa ccgtgcatac ggcgtggcgg ggaaaagcct gttcgacgat     1080

gggctgggag ccgcgcccgg cgtgccgagc ggacgttcga agttctcgcc ggatgtactg     1140

gaaacggtgc cggcgtcacc cggattgcgg cggccgtcgc tgggcgcagt ggaacgccag     1200

gattccggct atgacagcct tgatggggtg ggatcgcgat cgttctcgtt gggcgaggtg     1260

tccgacatgg ccgccgtgga agcggcggaa ctggaaatga cccggcaagt cttgcacgcc     1320

ggggcgcggc aggacgatgc cgagccgggc gtgagcggtg cgtcggcgca ctgggggcag     1380

cgggcgctgc agggcgccca ggcggtggcg gcggcgcagc ggctggttca tgccattgcc     1440

ctgatgacgc aattcggccg ggccggttcc accaacacgc cgcaggaagc ggcctcgttg     1500

tcggcggccg tgttcggctt gggcgaggcc agcagcgccg tggccgaaac cgtgagcggt     1560

tttttccgcg ggtcttcgcg ctgggccggc ggtttcggcg tggctggcgg cgcgatggcg     1620

ctgggaggcg gcatcgccgc ggccgttggc gccgggatgt cgttgaccga tgacgcgccg     1680

gccggacaga aggccgccgc cggcgccgag atcgcgctgc agttgacagg tggaacggtc     1740

gagctggctt cttccatcgc gttggcgctg gccgcggcgc gcggcgtgac cagcggcttg     1800

caggtggccg gggcgtcggc cggggcggct gccggcgcat tggccgcggc gctcagtccc     1860

atggagatct acggcctggt gcagcaatcg cactatgcgg atcagctgga caagctggcg     1920

caggaatcga gcgcatacgg ttacgagggc gacgccttgc tggcccagct gtatcgcgac     1980

aagacggccg ccgagggcgc cgtcgccggc gtctccgccg tcctgagcac ggtgggggcg     2040

gcggtgtcga tcgccgcggc ggccagcgtg gtaggggccc cggtggcggt ggtcacttcc     2100

ttgctgaccg gggctctcaa cggcatcctg cgcggcgtgc agcagcccat catcgaaaag     2160

ctggccaacg attacgctcg caagatcgac gagctgggcg ggccgcaagc gtacttcgag     2220

aaaaacctgc aggcgcgtca cgaacaactg gccaattcgg acggcctacg gaaaatgctg     2280

gccgacctgc aggccggttg gaacgccagc agcgtgatcg gggtgcagac gacagagatc     2340

tccaagtcgg cgctcgaact ggccgccatt accggcaacg cggacaacct gaaatccgtc     2400

gacgtgttcg tggaccgctt cgtccagggc gagcgggtgg ccggccagcc ggtggtcctc     2460

gacgtcgccg ccggcggcat cgatatcgcc agccgcaagg gcgagcggcc ggcgctgacg     2520

ttcatcacgc cgctggccgc gccaggagaa gagcagcgcc ggcgcacgaa aacgggcaag     2580

agcgaattca ccacattcgt cgagatcgtg ggcaagcagg accgctggcg catccgggac     2640

ggcgcggccg acaccaccat cgatctggcc aaggtggtgt cgcaactggt cgacgccaat     2700

ggcgtgctca agcacagcat caaactggat gtgatcggcg gagatggcga tgacgtcgtg     2760

cttgccaatg cttcgcgcat ccattatgac ggcggcgcgg gcaccaacac ggtcagctat     2820

gccgccctgg gtcgacagga ttccattacc gtgtccgccg acggggaacg tttcaacgtg     2880

cgcaagcagt tgaacaacgc caacgtgtat cgcgaaggcg tggctaccca gacaaccgcc     2940

tacggcaagc gcacggagaa tgtccaatac cgccatgtcg agctggcccg tgtcgggcaa     3000

ctggtggagg tcgacacgct cgagcatgtg cagcacatca tcggcggggc cggcaacgat     3060

tcgatcaccg gcaatgcgca cgacaacttc ctagccggcg ggtcgggcga cgacaggctg     3120

gatggcggcg ccggcaacga caccctggtt ggcggcgagg gccaaaacac ggtcatcggc     3180

ggcgccggcg acgacgtatt cctgcaggac ctgggggtat ggagcaacca gctcgatggc     3240

ggcgcgggcg tcgataccgt gaagtacaac gtgcaccagc cttccgagga gcgcctcgaa     3300

cgcatgggcg acacgggcat ccatgccgat cttcaaaagg gcacggtcga gaagtggccg     3360

gccctgaacc tgttcagcgt cgaccatgtc aagaatatcg agaatctgca cggctcccgc     3420

ctgaacgacc gcatcgccgg cgacgaccag gacaacgagc tctggggcca cgatggcaac     3480

gacacgatac gcggccgggg cggcgacgac atcctgcgcg gcggcctggg cctggacacg     3540

ctgtatggcg aggacggcaa cgacatcttc ctgcaggacg acgagaccgt cagcgatgac     3600

atcgacggcg gcgcggggct ggacaccgtc gactactccg ccatgatcca tccaggcagg     3660

atcgttgcgc cgcatgaata cggcttcggg atcgaggcgg acctgtccag ggaatgggtg     3720

cgcaaggcgt ccgcgctggg cgtggactat tacgataatg tccgcaatgt cgaaaacgtc     3780

atcggtacga gcatgaagga tgtgctcatc ggcgacgcgc aagccaatac cctgatgggc     3840

cagggcggcg acgataccgt gcgcggcggc gacggcgatg atctgctgtt cggcggcgac     3900

ggcaacgaca tgctgtatgg cgacgccggc aacgacaccc tctacggggg gctgggcgac     3960

gatacccttg aaggcggcgc gggcaacgat tggttcggcc agacgcaggc gcgcgagcat     4020

gacgtgctgc gcggcggaga tggggtggat accgtcgatt acagccagac cggcgcgcat     4080

gccggcattg ccgcgggtcg catcgggctg ggcatcctgg ctgacctggg cgccggccgc     4140

gtcgacaagc tgggcgaggc cggcagcagc gcctacgata cggtttccgg tatcgagaac     4200

gtggtgggca cggaactggc cgaccgcatc acgggcgatg cgcaggccaa cgtgctgcgc     4260

ggcgcgggtg gcgccgacgt gcttgcgggc ggcgagggcg acgatgtgct gctgggcggc     4320

gacggcgacg accagctgtc gggcgacgcc ggacgcgatc gcttgtacgg cgaagccggt     4380

gacgactggt tcttccagga tgccgccaat gccggcaatc tgctcgacgg cggcgacggc     4440

cgcgataccg tggatttcag cggcccgggc cggggcctcg acgccggcgc aaagggcgta     4500

ttcctgagct tgggcaaggg gttcgccagc ctgatggacg aacccgaaac cagcaacgtg     4560

ttgcgcaata tcgagaacgc cgtgggcagc gcgcgtgatg acgtgctgat cggcgacgca     4620

ggcgccaacg tcctcaatgg cctggcgggc aacgacgtgc tgtccggcgg cgctggcgac     4680

gatgtgctgc tgggcgacga gggctcggac ctgctcagcg gcgatgcggg caacgacgat     4740

ctgttcggcg ggcagggcga tgatacttat ctgttcgggg tcgggtacgg gcacgacacg     4800

atctacgaat cgggcggcgg ccatgacacc atccgcatca acgcgggggc ggaccagctg     4860

tggttcgcgc gccagggcaa cgacctggag atccgcattc tcggcaccga cgatgcactt     4920

accgtgcacg actggtatcg cgacgccgat caccgggtgg aaatcatcca tgccgccaac     4980

caggcggtag accaggcagg catcgaaaag ctggtcgagg caatggcgca gtatccggac     5040

cccggcgcgg cggcggctgc cccgccggcg gcgcgcgtgc cggacacgct gatgcagtcc     5100

ctggctgtca actggcgctg a                                               5121


<210>  5
<211>  5223
<212>  DNA
<213>  Bordetella parapertussis / cyaA gene

<400>  5
gtgctggatg tttggttctt gcagaaggat gaggttctga gcgctacaca ccggttgcgt       60

cggtgcgaat ccgttcaatc gactacttat cgacagatcc acatgcagca atcgcatcag      120

gctggttacg caaacgccgc cgaccgggag tctggcatcc ccgcagccgt actcgatggc      180

atcaaggccg tggcgaagga aaaaaacgcc acattgatgt tccgcctggt caacccccat      240

tccaccagcc tgattgccga aggggtggcc accaaaggat tgggcgtgca cgccaagtcg      300

tccgattggg ggttgcaggc gggctacatt cccgtcaacc cgaatctttc caaactgttc      360

ggccgtgcgc ccgaggtgat cgcgcgggcc gacaacgacg tcaacagcag cctggcgcat      420

ggccataccg cggtcgacct gacgctgtcg aaagagcggc ttgactatct gcggcaagcg      480

ggcctggtca ccggcatggc cgatggcgtg gtcgcgagca accacgcagg ctacgagcag      540

ttcgagtttc gcgtgaagga aacctcggac gggcgctatg ccgtgcagta tcgccgcaag      600

ggcggcgacg atttcgaggc ggtcaaggtg atcggcaatg ccgccggtat tccactgacg      660

gcggatatcg acatgttcgc catcatgccg catctgtcca acttccgcga ctcggcgcgc      720

agttcggtga ccagcggcga ttcggtgacc gattacctgg cgcgcacgcg gcgggccgcc      780

agcgaggcca cgggcggcct ggatcgcgaa cgcatcgact tgttgtggaa aatcgctcgc      840

gccggcgccc gttccgcagt gggcaccgag gcgcgtcgcc agttccgcta cgacggcgac      900

atgaatatcg gcgtgatcac cgatttcgag ctggaagtgc gcaatgcgct gaacaggcgg      960

gcgcacgccg tcggcgcgca ggacgtggtc cagcatggca ctgagcagaa caatcctttc     1020

ccggaggcag atgagaagat tttcgtcgta tcggccaccg gtgaaagcca gatgctcacg     1080

cgcgggcaac tgaaggaata cattggccag cagcgcggcg agggctatgt cttctacgag     1140

aaccgtgcat acggcgtggc ggggaaaagc ctgttcgacg atgggctggg agccgcgccc     1200

ggcgtgccgg gcggacgttc gaagtcctcg ccggatgtac tggaaacggt gccggcgtca     1260

cccggattgc ggcggccgtc gctgggcgca gtggaacgcc aggattccgg ctatgacagc     1320

cttgatgggg tgggatcgcg atcgttctcg ttgggcgagg tgtccgacat ggccgccgtg     1380

gaagcggcgg aactggaaat gacccggcaa gtcttgcacg ccggggcgcg gcaggacgat     1440

gccgagccgg gcgtgagcgg tgcgtcggcg cactgggggc agcgggcgct gcagggcgcc     1500

caggcggtgg cggcggcgca gcggctggtt catgccattg ccctgatgac gcaattcggc     1560

cgggccggtt ccaccaacac gccgcaggaa gcggcctcgt tgtcggcggc cgtgttcggc     1620

ttgggcgagg ccagcagcgc cgtggccgaa accgtgagcg gttttttccg cgggtcttcg     1680

cgctgggccg gcggtttcgg cgtggctggc ggcgcgatgg cgctgggagg cggcatcgcc     1740

gcggccgttg gcgccgggat gtcgttgacc gatgacgcgc cggccggaca gaaggccgcc     1800

gtcggcgccg agatcgcgct gcagttgaca ggtggaacgg tcgagctggc ttcttccatc     1860

gcgttggcgc tggccgcggc gcgcggcgtg accagcggct tgcaggtggc gggggcgtcg     1920

gccggggcgg ctgccggcgc attggccgcg gcgctcagtc ccatggagat ctacggcctg     1980

gtgcagcaat cgcactatgc ggatcagctg gacaagctgg cgcaggaatc gagcgcatac     2040

ggttacgagg gcgacgcctt gctggcccag ctgtatcgcg acaagacggc cgccgagggc     2100

gccgtcgccg gcgtctccgc cgtcctgagc acggtggggg cggcggtgtc gatcgccgcg     2160

gcggccagcg tggtaggcgc cccggtggcg gtggtcactt ccttgttgac cggggctctc     2220

aacggcatcc tgcgcggcgt gcagcagccc atcatcgaaa agctggccaa tgattacgct     2280

cgcaagatcg acgagctggg cgggccgcaa gcgtacttcg agaaaaacct gcaggcgcgt     2340

cacgaacaac tggccaattc ggacggccta cggaaaatgc tggctgacct gcaggccggg     2400

tggaacgcca gcagcgtgat cggggtgcag acgacagaga tttccaagtc ggcgctcgaa     2460

ctggccgcca ttaccggcaa cgcggacaac ctgaaatccg ccgacgtgtt cgtggaccgc     2520

ttcatccagg gcgagcgggt ggccggccag ccggtggtac tcgacgtcgc cgccggcggc     2580

atcgatatcg ccagccgcaa gggcgagcgg ccggcgctga cgttcatcac gccgctggcc     2640

gcgccaggag aagagcagcg ccggcgcacg aagacgggca agagcgaatt caccacattc     2700

gtcgagatcg tgggcaagca ggaccgctgg cgcatccggg acggcgcggc cgacaccacc     2760

atcgatctgg ccaaggtggt gtcgcaactg gtcgacgcca atggcgtgct caagcacagc     2820

atcaaactgg aggtgatcgg cggagatggc gatgatgtcg tgcttgccaa tgcttcgcgc     2880

atccattacg acggcggcgc gggaaccaac acggtcagct atgccgccct gggccgacag     2940

gattccatta ccgtgtccgc cgacggggaa cgtttcaacg tgcgcaagca gttgaacaac     3000

gccaacgtgt atcgcgaagg cgtggctacc cagaaaaccg cctacggcaa gcgcacggag     3060

aatgtccaat accgccatgt cgagctggcc cgtgtcgggc aactggtgga ggtcgacacg     3120

ctcgagcatg tgcagcacat catcggcggg gccggcaacg attcgatcac cggcaatgcg     3180

cacgacaact tcctggccgg cggggcgggc gacgacaggc tggatggcgg cgccggcaac     3240

gacacactgg tcggcggcga gggccacaac acggtcgtcg gcggcgctgg cgacgacgta     3300

ttcctgcagg acctgggggt atggagcaac cagctcgatg gcggcgcggg cgtcgatacc     3360

gtgaagtaca acgtgcacca gccttccgag gaacgcctcg aacgcatggg cgacacgggc     3420

atccatgccg atcttcaaaa gggcacggtc gagaagtggc cggccctgaa cctgttcagc     3480

gtcgaccatg tcaagaatat cgagaatctg cacggctcca gcctgaacga cagcatcgcc     3540

ggcgacgacc gggacaacga gctctggggc gacgatggca acgacacgat acacggccgg     3600

ggcggcgacg atatcctgcg cggcggcctg ggcctggaca cgctgtatgg cgaggacggc     3660

aacgacatct tcctgcagga cgacgagacc gtcagcgatg acatcgacgg cggcgcgggg     3720

ctggacaccg tcgactattc cgccatgatc catgcaggca agatcgttgc gccgcatgaa     3780

tacggcttcg ggatcgaggc ggacctgtcc gaagggtggg tgcgcaaggc ggcccggcgc     3840

ggcatgggct actacgacag tgtccgcagt gtcgaaaacg tcatcggcac gagcatgaag     3900

gatgtgctca tcggcgacgc gcaagccaat accctgatgg gccagggcgg cgacgatacc     3960

gtgcgcggcg gcgacggcga tgatctgctg ttcggcggcg acggcaacga catgctgtat     4020

ggagacgccg gcaacgacac cctctacgga gggctgggcg acgataccct tgaaggcggc     4080

gcgggcaacg attggttcgg ccagacgccg gcgcgcgagc atgacgtgct gcgcggcggg     4140

gctggggtgg ataccgtgga ttacagccag gcgggcgcgc atgccggcgt tgccacgggt     4200

cgcatcgggc tgggtattct ggcggacctg ggcgccggcc gcgtcgacaa gctgggcgag     4260

gccggcagca gcgcctacga tacggtttcc ggcatcgaaa atgtggtggg cacggaactg     4320

gccgaccgca tcacgggcga tgcgcaggcc aacgtactgc gcggcgcggg tggtgccgac     4380

gtgcttgcgg gcggcgaggg cgacgatgtg ctgctgggcg gcgagggcga tgaccagctg     4440

tcgggcgacg ccggacgcga ccgcttgtac ggcgaagccg gtgacgactg gttcttccag     4500

gatgccgcca atgccggcaa tctgctcgac ggtggtgacg gcaacgatac cgtggatttc     4560

agcggcccgg gccggggcct cgacgccggc gcaaagggcg tattcctgag cctgggcaag     4620

gggttcgcca gcctgatgga cgaacccgaa accagcaacg tgttgcgcca tatcgagaac     4680

gccgtgggca gcgtgcgtga tgacgtgctg atcggcgacg caggcgccaa cgtcctcaat     4740

ggcctggcgg gcaacgacgt gttgtccggc ggcgccggcg acgatgtgct gctgggcgac     4800

gagggctcgg acctgctcag cggcgatgcg ggcaacgacg atctgttcgg cgggcagggc     4860

gatgatacct atctgttcgg ggccgggtac ggacatgaca cgatctacga atcgggcggc     4920

ggccatgaca ccatccgtat caacgcgggg gcggaccagc tgtggtttgc gcgccagggc     4980

aacgacctgg agatccgcat tcttggcacc gacgatgcac ttaccgtgca cgactggtat     5040

cgcgacgccg atcaccgggt ggaagccatc catgccgcca accaggccat agacccggcc     5100

ggcatcgaaa agctggtcga ggcaatggcg cagtacccgg accccggcgc ggcggcggct     5160

gccccgccgg cggcgcgcgt gccggacacg ctgatgcagt ccctggctgt caactggcgc     5220

tga                                                                   5223


<210>  6
<211>  5223
<212>  DNA
<213>  Bordetella bronchiseptica / cyaA gene

<400>  6
gtgctggatg tttggttctt gcagaaggat gaggttctga gcgctacaca ccggttgcgt       60

cggtgcgaat ccgttcaatc gactacttat cgacagatcc acatgcagca atcgcatcag      120

gctggttacg caaacgccgc cgaccgggag tctggcatcc ccgcagccgt actcgatggc      180

atcaaggccg tggcgaagga aaaaaacgcc acattgatgt tccgcctggt caacccccat      240

tccaccagcc tgattgccga aggggtggcc accaaaggat tgggcgtgca cgccaagtcg      300

tccgattggg ggttgcaggc gggctacatt cccgtcaacc cgaatctttc caaactgttc      360

ggccgtgcgc ccgaggtgat cgcgcgggcc gacaacgacg tcaacagcag cctggcgcat      420

ggccataccg cggtcgacct gacgctgtcg aaagagcggc ttgactatct gcggcaagcg      480

ggcctggtca ccggcatggc cgatggcgtg gtcgcgagca accacgcagg ctacgagcag      540

ttcgagtttc gcgtgaagga aacctcggac gggcgctatg ccgtgcagta tcgccgcaag      600

ggcggcgacg atttcgaggc ggtcaaggtg atcggcaatg ccgccggtat tccactgacg      660

gcggatatcg acatgttcgc catcatgccg catctgtcca acttccgcga ctcggcgcgc      720

agttcggtga ccagcggcga ttcggtgacc gattacctgg cgcgcacgcg gcgggccgcc      780

agcgaggcca cgggcggcct ggatcgcgaa cgcatcgact tgttgtggaa aatcgctcgc      840

gccggcgccc gttccgcagt gggcaccgag gcgcgtcgcc agttccgcta cgacggcgac      900

atgaatatcg gcgtgatcac cgatttcgag ctggaagtgc gcaatgcgct gaacaggcgg      960

gcgcacgccg tcggcgcgca ggacgtggtc cagcatggca ctgagcagaa caatcctttc     1020

ccggaggcag atgagaagat tttcgtcgta tcggccaccg gtgaaagcca gatgctcacg     1080

cgcgggcaac tgaaggaata cattggccag cagcgcggcg agggctatgt cttctacgag     1140

aaccgtgcgt acggcgtggc ggggaaaagc ctgttcgacg atgggctggg agccgcgccc     1200

ggcgtgccgg gcggacgttc gaagtcctcg ccggatgtac tggaaacggt gccggcgtca     1260

cccggattgc ggcggccgtc gctgggcgca gtggaacgcc aggattccgg ctatgacagc     1320

cttgatgggg tgggatcgcg atcgttctcg ttgggcgagg tgtccgacat ggccgccgtg     1380

gaagcggcgg aactggaaat gacccggcaa gtcttgcacg ccggggcgcg gcaggacgat     1440

gccgagccgg gcgtgagcgg tgcgtcggcg cactgggggc agcgggcgct gcagggcgcc     1500

caggcggtgg cggcggcgca gcggctggtt catgccattg ccctgatgac gcaattcggc     1560

cgggccggtt ccaccaacac gccgcaggaa gcggcctcgt tgtcggcggc cgtgttcggc     1620

ttgggcgagg ccagcagcgc cgtggccgaa accgtgagcg gttttttccg cgggtcttcg     1680

cgctgggccg gcggtttcgg cgtggctggc ggcgcgatgg cgctgggagg cggcatcgcc     1740

gcggccgttg gcgccgggat gtcgttgacc gatgacgcgc cggccggaca gaaggccgcc     1800

gccggcgccg agatcgcgct gcagttgaca ggtggaacgg tcgagctggc ttcttccatc     1860

gcgttggcgc tggccgcggc gcgcggcgtg accagcggct tgcaggtggc gggggcgtcg     1920

gccggggcgg ctgccggcgc attggccgcg gcgctcagtc ccatggagat ctacggcctg     1980

gtgcagcaat cgcactatgc ggatcagctg gacaagctgg cgcaggaatc gagcgcatac     2040

ggttacgagg gcgacgcctt gctggcccag ctgtatcgcg acaagacggc cgccgagggc     2100

gccgtcgccg gcgtctccgc cgtcctgagc acggtggggg ctgcggtgtc gatcgccgcg     2160

gcggccagcg tggtaggcgc cccggtggcg gtggtcactt ccttgttgac cggggctctc     2220

aacggcatcc tgcgcggcgt gcagcagccc atcatcgaaa agctggccaa tgattacgct     2280

cgcaagatcg acgagctggg cgggccgcaa gcgtacttcg agaaaaacct gcaggcgcgt     2340

cacgaacaac tggccaattc ggacggccta cggaaaatgc tggccgacct gcaggccggg     2400

tggaacgcca gcagcgtgat cggggtgcag acgacagaga tttccaagtc ggcgctcgaa     2460

ctggccgcca ttaccggcaa cgcggacaac ctgaaatccg ccgacgtgtt cgtggaccgc     2520

ttcatccagg gcgagcgggt ggccggccag ccggtggtac tcgacgtcgc cgccggcggc     2580

atcgatatcg ccagccgcaa gggcgagcgg ccggcgctga cgttcatcac gccgctggcc     2640

gcgccaggag aagagcagcg ccggcgcacg aaaacgggca agagcgaatt caccacattc     2700

gtcgagatcg tgggcaagca ggaccgctgg cgcatccggg acggcgcggc cgacaccacc     2760

atcgatctgg ccaaggtggt gtcgcaactg gtcgacgcca atggcgtgct caagcacagc     2820

atcaaactgg aggtgatcgg cggagatggc gatgatgtcg tgcttgccaa tgcttcgcgc     2880

atccattacg acggcggcgc gggaaccaac acggtcagct atgccgccct gggccgacag     2940

gattccatta ccgtgtccgc cgacggggaa cgtttcaacg tgcgcaagca gttgaacaac     3000

gccaacgtgt atcgcgaagg cgtggctacc cagaaaaccg cctacggcaa gcgcacggag     3060

aatgtccaat accgccatgt cgagctggcc cgtgtcgggc aactggtgga ggtcgacacg     3120

ctcgagcatg tgcagcacat catcggcggg gccggcaacg attcgatcac cggcaatgcg     3180

cacgacaact tcctggccgg cggggcgggc gacgacaggc tggatggcgg cgccggcaac     3240

gacacactgg tcggcggcga gggccacaac acggtcgtcg gcggcgctgg cgacgacgta     3300

ttcctgcagg acctgggggt atggagcaac cagctcgatg gcggcgcggg cgtcgatacc     3360

gtgaagtaca acgtgcacca gccttccgag gaacgcctcg aacgcatggg cgacacgggc     3420

atccatgccg atcttcaaaa gggcacggtc gagaagtggc cggccctgaa cctgttcagc     3480

gtcgaccatg tcaagaatat cgagaatctg cacggctcca gcctgaacga cagcatcgcc     3540

ggcgacgacc gggacaacga gctctggggc gacgatggca acgacacgat acacggccgg     3600

ggcggcgacg atatcctgcg cggcggcctg ggcctggaca cgctgtatgg cgaggacggc     3660

aacgacatct tcctgcagga cgacgagacc gtcagcgatg acatcgacgg tggcgcggga     3720

ctggacaccg tcgactattc cgccatgatc catgcaggca agatcgttgc gccgcatgaa     3780

tacggcttcg ggatcgaggc ggacctgtcc gaagggtggg tgcgcaaggc ggcccggcgc     3840

ggcatggact actacgacag tgtccgcagt gtcgaaaacg tcatcggcac gagcatgaag     3900

gatgtgctca tcggcgacgc gcaagccaat accctgatgg gccagggcgg cgacgatacc     3960

gtgcgcggcg gcgacggcga tgatctgctg ttcggcggcg acggcaacga catgctgtat     4020

ggagacgccg gcaacgacac cctctacgga gggctgggcg acgataccct tgaaggcggc     4080

gcgggcaacg attggttcgg ccagacgccg gcgcgcgagc atgacgtgct gcgcggcggg     4140

gctggggtgg ataccgtgga ttacagccag gcgggcgcgc atgccggcgt tgccacgggt     4200

cgcatcgggc tgggtattct ggcggacctg ggcgccggcc gcgtcgacaa gctgggcgag     4260

gccggcagca gcgcctacga tacggtttcc ggcatcgaaa atgtggtggg cacggaactg     4320

gccgaccgca tcacgggcga tgcgcaggcc aacgtactgc gcggcgcggg tggcgccgac     4380

gtgcttgcgg gcggcgaggg cgacgatgtg ctgctgggcg gcgacggcga cgaccagctg     4440

tcgggcgacg ccggacgcga ccgcttgtac ggcgaagccg gtgacgactg gttcttccag     4500

gatgccgcca atgccggcaa tctgctcgac ggtggtgacg gcaacgatac cgtggatttc     4560

agcggcccgg gccggggcct cgacgccggc gcaaagggcg tattcctgag cctgggcaag     4620

gggttcgcca gcctgatgga cgaacccgaa accagcaacg tgttgcgcca tatcgagaac     4680

gccgtgggca gcgtgcgtga tgacgtgctg atcggcgacg caggcgccaa cgtcctcaat     4740

ggcctggcgg gcaacgacgt gctgtccggc ggcgccggcg acgatgtgct gctgggcgac     4800

gagggctcgg acctgctcag cggcgatgcg ggcaacgacg atctgttcgg cgggcagggc     4860

gatgatacct atctgttcgg ggccgggtac ggacatgaca cgatctacga atcgggcggc     4920

ggccatgaca ccatccgtat caacgcgggg gcggaccagc tgtggtttgc gcgccagggc     4980

aacgacctgg agatccgcat tcttggcacc gacgatgcac ttaccgtgca cgactggtat     5040

cgcgacgccg atcaccgggt ggaagccatc catgccgcca accaggccat agacccggcc     5100

ggcatcgaaa agctggtcga ggcaatggcg cagtacccgg accccggcgc ggcggcggct     5160

gccccgccgg cggcgcgcgt gccggacacg ctgatgcagt ccctggctgt caactggcgc     5220

tga                                                                   5223


<210>  7
<211>  1872
<212>  PRT
<213>  Artificial

<220>
<223>  CyaA - mage A3 vector

<400>  7

Met Gln Gln Ser His Gln Ala Gly Tyr Ala Asn Ala Ala Asp Arg Glu 
1               5                   10                  15      


Ser Gly Ile Pro Ala Ala Val Leu Asp Gly Ile Lys Ala Val Ala Lys 
            20                  25                  30          


Glu Lys Asn Ala Thr Leu Met Phe Arg Leu Val Asn Pro His Ser Thr 
        35                  40                  45              


Ser Leu Ile Ala Glu Gly Val Ala Thr Lys Gly Leu Gly Val His Ala 
    50                  55                  60                  


Lys Ser Ser Asp Trp Gly Leu Gln Ala Gly Tyr Ile Pro Val Asn Pro 
65                  70                  75                  80  


Asn Leu Ser Lys Leu Phe Gly Arg Ala Pro Glu Val Ile Ala Arg Ala 
                85                  90                  95      


Asp Asn Asp Val Asn Ser Ser Leu Ala His Gly His Thr Ala Val Asp 
            100                 105                 110         


Leu Thr Leu Ser Lys Glu Arg Leu Asp Tyr Leu Arg Gln Ala Gly Leu 
        115                 120                 125             


Val Thr Gly Met Ala Asp Gly Val Val Ala Ser Asn His Ala Gly Tyr 
    130                 135                 140                 


Glu Gln Phe Glu Phe Arg Val Lys Glu Thr Ser Asp Gly Arg Tyr Ala 
145                 150                 155                 160 


Val Gln Tyr Arg Arg Lys Gly Gly Asp Asp Phe Glu Ala Val Lys Val 
                165                 170                 175     


Ile Gly Asn Ala Ala Gly Ile Pro Leu Thr Ala Asp Leu Gln Ile Asp 
            180                 185                 190         


Met Phe Ala Ile Met Pro His Leu Ser Asn Phe Arg Asp Ser Ala Arg 
        195                 200                 205             


Ser Ser Val Thr Ser Gly Asp Ser Val Thr Asp Tyr Leu Ala Arg Thr 
    210                 215                 220                 


Arg Arg Ala Ser Thr Phe Pro Asp Leu Glu Ser Glu Phe Gln Ala Ala 
225                 230                 235                 240 


Leu Ser Arg Lys Val Ala Glu Leu Val His Phe Leu Leu Leu Lys Tyr 
                245                 250                 255     


Arg Ala Arg Glu Pro Val Thr Lys Ala Glu Met Leu Gly Ser Val Val 
            260                 265                 270         


Gly Asn Trp Gln Tyr Phe Phe Pro Val Ile Phe Ser Lys Ala Ser Ser 
        275                 280                 285             


Ser Leu Gln Leu Val Phe Gly Ile Glu Leu Met Glu Val Asp Pro Ile 
    290                 295                 300                 


Gly His Leu Tyr Ile Phe Gly Thr Arg Ala Arg Leu Lys Leu Leu Trp 
305                 310                 315                 320 


Lys Ile Ala Arg Ala Gly Ala Arg Ser Ala Val Gly Thr Glu Ala Arg 
                325                 330                 335     


Arg Gln Phe Arg Tyr Asp Gly Asp Met Asn Ile Gly Val Ile Thr Asp 
            340                 345                 350         


Phe Glu Leu Glu Val Arg Asn Ala Leu Asn Arg Arg Ala His Ala Val 
        355                 360                 365             


Gly Ala Gln Asp Val Val Gln His Gly Thr Glu Gln Asn Asn Pro Phe 
    370                 375                 380                 


Pro Glu Ala Asp Glu Lys Ile Phe Val Val Ser Ala Thr Gly Leu Gly 
385                 390                 395                 400 


Asp Asn Gln Ile Met Pro Lys Ala Gly Leu Leu Ile Ile Val Leu Ala 
                405                 410                 415     


Ile Ile Ala Arg Glu Gly Asp Cys Ala Pro Glu Glu Lys Ile Pro Lys 
            420                 425                 430         


Lys Leu Leu Thr Gln His Phe Val Gln Glu Asn Tyr Leu Glu Tyr Arg 
        435                 440                 445             


Gln Val Pro Gly Ser Asp Pro Ala Cys Tyr Glu Phe Leu Trp Gly Pro 
    450                 455                 460                 


Arg Ala Leu Val Glu Thr Ser Tyr Val Lys Val Leu His His Met Val 
465                 470                 475                 480 


Lys Ile Ser Gly Thr Ser Glu Ser Gln Met Leu Thr Arg Gly Gln Leu 
                485                 490                 495     


Lys Glu Tyr Ile Gly Gln Gln Arg Gly Glu Gly Tyr Val Phe Tyr Glu 
            500                 505                 510         


Asn Arg Ala Tyr Gly Val Ala Gly Lys Ser Leu Phe Asp Asp Gly Leu 
        515                 520                 525             


Gly Ala Ala Pro Gly Val Pro Ser Gly Arg Ser Lys Phe Ser Pro Asp 
    530                 535                 540                 


Val Leu Glu Thr Val Pro Ala Ser Pro Gly Leu Arg Arg Pro Ser Leu 
545                 550                 555                 560 


Gly Ala Val Glu Arg Gln Asp Ser Gly Tyr Asp Ser Leu Asp Gly Val 
                565                 570                 575     


Gly Ser Arg Ser Phe Ser Leu Gly Glu Val Ser Asp Met Ala Ala Val 
            580                 585                 590         


Glu Ala Ala Glu Leu Glu Met Thr Arg Gln Val Leu His Ala Gly Ala 
        595                 600                 605             


Arg Gln Asp Asp Ala Glu Pro Gly Val Ser Gly Ala Ser Ala His Trp 
    610                 615                 620                 


Gly Gln Arg Ala Leu Gln Gly Ala Gln Ala Val Ala Ala Ala Gln Arg 
625                 630                 635                 640 


Leu Val His Ala Ile Ala Leu Met Thr Gln Phe Gly Arg Ala Gly Ser 
                645                 650                 655     


Thr Asn Thr Pro Gln Glu Ala Ala Ser Leu Ser Ala Ala Val Phe Gly 
            660                 665                 670         


Leu Gly Glu Ala Ser Ser Ala Val Ala Glu Thr Val Ser Gly Phe Phe 
        675                 680                 685             


Arg Gly Ser Ser Arg Trp Ala Gly Gly Phe Gly Val Ala Gly Gly Ala 
    690                 695                 700                 


Met Ala Leu Gly Gly Gly Ile Ala Ala Ala Val Gly Ala Gly Met Ser 
705                 710                 715                 720 


Leu Thr Asp Asp Ala Pro Ala Gly Gln Lys Ala Ala Ala Gly Ala Glu 
                725                 730                 735     


Ile Ala Leu Gln Leu Thr Gly Gly Thr Val Glu Leu Ala Ser Ser Ile 
            740                 745                 750         


Ala Leu Ala Leu Ala Ala Ala Arg Gly Val Thr Ser Gly Leu Gln Val 
        755                 760                 765             


Ala Gly Ala Ser Ala Gly Ala Ala Ala Gly Ala Leu Ala Ala Ala Leu 
    770                 775                 780                 


Ser Pro Met Glu Ile Tyr Gly Leu Val Gln Gln Ser His Tyr Ala Asp 
785                 790                 795                 800 


Gln Leu Asp Lys Leu Ala Gln Glu Ser Ser Ala Tyr Gly Tyr Glu Gly 
                805                 810                 815     


Asp Ala Leu Leu Ala Gln Leu Tyr Arg Asp Lys Thr Ala Ala Glu Gly 
            820                 825                 830         


Ala Val Ala Gly Val Ser Ala Val Leu Ser Thr Val Gly Ala Ala Val 
        835                 840                 845             


Ser Ile Ala Ala Ala Ala Ser Val Val Gly Ala Pro Val Ala Val Val 
    850                 855                 860                 


Thr Ser Leu Leu Thr Gly Ala Leu Asn Gly Ile Leu Arg Gly Val Gln 
865                 870                 875                 880 


Gln Pro Ile Ile Glu Lys Leu Ala Asn Asp Tyr Ala Arg Lys Ile Asp 
                885                 890                 895     


Glu Leu Gly Gly Pro Gln Ala Tyr Phe Glu Lys Asn Leu Gln Ala Arg 
            900                 905                 910         


His Glu Gln Leu Ala Asn Ser Asp Gly Leu Arg Lys Met Leu Ala Asp 
        915                 920                 925             


Leu Gln Ala Gly Trp Asn Ala Ser Ser Val Ile Gly Val Gln Thr Thr 
    930                 935                 940                 


Glu Ile Ser Lys Ser Ala Leu Glu Leu Ala Ala Ile Thr Gly Asn Ala 
945                 950                 955                 960 


Asp Asn Leu Lys Ser Val Asp Val Phe Val Asp Arg Phe Val Gln Gly 
                965                 970                 975     


Glu Arg Val Ala Gly Gln Pro Val Val Leu Asp Val Ala Ala Gly Gly 
            980                 985                 990         


Ile Asp Ile Ala Ser Arg Lys Gly  Glu Arg Pro Ala Leu  Thr Phe Ile 
        995                 1000                 1005             


Thr Pro  Leu Ala Ala Pro Gly  Glu Glu Gln Arg Arg  Arg Thr Lys 
    1010                 1015                 1020             


Thr Gly  Lys Ser Glu Phe Thr  Thr Phe Val Glu Ile  Val Gly Lys 
    1025                 1030                 1035             


Gln Asp  Arg Trp Arg Ile Arg  Asp Gly Ala Ala Asp  Thr Thr Ile 
    1040                 1045                 1050             


Asp Leu  Ala Lys Val Val Ser  Gln Leu Val Asp Ala  Asn Gly Val 
    1055                 1060                 1065             


Leu Lys  His Ser Ile Lys Leu  Asp Val Ile Gly Gly  Asp Gly Asp 
    1070                 1075                 1080             


Asp Val  Val Leu Ala Asn Ala  Ser Arg Ile His Tyr  Asp Gly Gly 
    1085                 1090                 1095             


Ala Gly  Thr Asn Thr Val Ser  Tyr Ala Ala Leu Gly  Arg Gln Asp 
    1100                 1105                 1110             


Ser Ile  Thr Val Ser Ala Asp  Gly Glu Arg Phe Asn  Val Arg Lys 
    1115                 1120                 1125             


Gln Leu  Asn Asn Ala Asn Val  Tyr Arg Glu Gly Val  Ala Thr Gln 
    1130                 1135                 1140             


Thr Thr  Ala Tyr Gly Lys Arg  Thr Glu Asn Val Gln  Tyr Arg His 
    1145                 1150                 1155             


Val Glu  Leu Ala Arg Val Gly  Gln Leu Val Glu Val  Asp Thr Leu 
    1160                 1165                 1170             


Glu His  Val Gln His Ile Ile  Gly Gly Ala Gly Asn  Asp Ser Ile 
    1175                 1180                 1185             


Thr Gly  Asn Ala His Asp Asn  Phe Leu Ala Gly Gly  Ser Gly Asp 
    1190                 1195                 1200             


Asp Arg  Leu Asp Gly Gly Ala  Gly Asn Asp Thr Leu  Val Gly Gly 
    1205                 1210                 1215             


Glu Gly  Gln Asn Thr Val Ile  Gly Gly Ala Gly Asp  Asp Val Phe 
    1220                 1225                 1230             


Leu Gln  Asp Leu Gly Val Trp  Ser Asn Gln Leu Asp  Gly Gly Ala 
    1235                 1240                 1245             


Gly Val  Asp Thr Val Lys Tyr  Asn Val His Gln Pro  Ser Glu Glu 
    1250                 1255                 1260             


Arg Leu  Glu Arg Met Gly Asp  Thr Gly Ile His Ala  Asp Leu Gln 
    1265                 1270                 1275             


Lys Gly  Thr Val Glu Lys Trp  Pro Ala Leu Asn Leu  Phe Ser Val 
    1280                 1285                 1290             


Asp His  Val Lys Asn Ile Glu  Asn Leu His Gly Ser  Arg Leu Asn 
    1295                 1300                 1305             


Asp Arg  Ile Ala Gly Asp Asp  Gln Asp Asn Glu Leu  Trp Gly His 
    1310                 1315                 1320             


Asp Gly  Asn Asp Thr Ile Arg  Gly Arg Gly Gly Asp  Asp Ile Leu 
    1325                 1330                 1335             


Arg Gly  Gly Leu Gly Leu Asp  Thr Leu Tyr Gly Glu  Asp Gly Asn 
    1340                 1345                 1350             


Asp Ile  Phe Leu Gln Asp Asp  Glu Thr Val Ser Asp  Asp Ile Asp 
    1355                 1360                 1365             


Gly Gly  Ala Gly Leu Asp Thr  Val Asp Tyr Ser Ala  Met Ile His 
    1370                 1375                 1380             


Pro Gly  Arg Ile Val Ala Pro  His Glu Tyr Gly Phe  Gly Ile Glu 
    1385                 1390                 1395             


Ala Asp  Leu Ser Arg Glu Trp  Val Arg Lys Ala Ser  Ala Leu Gly 
    1400                 1405                 1410             


Val Asp  Tyr Tyr Asp Asn Val  Arg Asn Val Glu Asn  Val Ile Gly 
    1415                 1420                 1425             


Thr Ser  Met Lys Asp Val Leu  Ile Gly Asp Ala Gln  Ala Asn Thr 
    1430                 1435                 1440             


Leu Met  Gly Gln Gly Gly Asp  Asp Thr Val Arg Gly  Gly Asp Gly 
    1445                 1450                 1455             


Asp Asp  Leu Leu Phe Gly Gly  Asp Gly Asn Asp Met  Leu Tyr Gly 
    1460                 1465                 1470             


Asp Ala  Gly Asn Asp Thr Leu  Tyr Gly Gly Leu Gly  Asp Asp Thr 
    1475                 1480                 1485             


Leu Glu  Gly Gly Ala Gly Asn  Asp Trp Phe Gly Gln  Thr Gln Ala 
    1490                 1495                 1500             


Arg Glu  His Asp Val Leu Arg  Gly Gly Asp Gly Val  Asp Thr Val 
    1505                 1510                 1515             


Asp Tyr  Ser Gln Thr Gly Ala  His Ala Gly Ile Ala  Ala Gly Arg 
    1520                 1525                 1530             


Ile Gly  Leu Gly Ile Leu Ala  Asp Leu Gly Ala Gly  Arg Val Asp 
    1535                 1540                 1545             


Lys Leu  Gly Glu Ala Gly Ser  Ser Ala Tyr Asp Thr  Val Ser Gly 
    1550                 1555                 1560             


Ile Glu  Asn Val Val Gly Thr  Glu Leu Ala Asp Arg  Ile Thr Gly 
    1565                 1570                 1575             


Asp Ala  Gln Ala Asn Val Leu  Arg Gly Ala Gly Gly  Ala Asp Val 
    1580                 1585                 1590             


Leu Ala  Gly Gly Glu Gly Asp  Asp Val Leu Leu Gly  Gly Asp Gly 
    1595                 1600                 1605             


Asp Asp  Gln Leu Ser Gly Asp  Ala Gly Arg Asp Arg  Leu Tyr Gly 
    1610                 1615                 1620             


Glu Ala  Gly Asp Asp Trp Phe  Phe Gln Asp Ala Ala  Asn Ala Gly 
    1625                 1630                 1635             


Asn Leu  Leu Asp Gly Gly Asp  Gly Arg Asp Thr Val  Asp Phe Ser 
    1640                 1645                 1650             


Gly Pro  Gly Arg Gly Leu Asp  Ala Gly Ala Lys Gly  Val Phe Leu 
    1655                 1660                 1665             


Ser Leu  Gly Lys Gly Phe Ala  Ser Leu Met Asp Glu  Pro Glu Thr 
    1670                 1675                 1680             


Ser Asn  Val Leu Arg Asn Ile  Glu Asn Ala Val Gly  Ser Ala Arg 
    1685                 1690                 1695             


Asp Asp  Val Leu Ile Gly Asp  Ala Gly Ala Asn Val  Leu Asn Gly 
    1700                 1705                 1710             


Leu Ala  Gly Asn Asp Val Leu  Ser Gly Gly Ala Gly  Asp Asp Val 
    1715                 1720                 1725             


Leu Leu  Gly Asp Glu Gly Ser  Asp Leu Leu Ser Gly  Asp Ala Gly 
    1730                 1735                 1740             


Asn Asp  Asp Leu Phe Gly Gly  Gln Gly Asp Asp Thr  Tyr Leu Phe 
    1745                 1750                 1755             


Gly Val  Gly Tyr Gly His Asp  Thr Ile Tyr Glu Ser  Gly Gly Gly 
    1760                 1765                 1770             


His Asp  Thr Ile Arg Ile Asn  Ala Gly Ala Asp Gln  Leu Trp Phe 
    1775                 1780                 1785             


Ala Arg  Gln Gly Asn Asp Leu  Glu Ile Arg Ile Leu  Gly Thr Asp 
    1790                 1795                 1800             


Asp Ala  Leu Thr Val His Asp  Trp Tyr Arg Asp Ala  Asp His Arg 
    1805                 1810                 1815             


Val Glu  Ile Ile His Ala Ala  Asn Gln Ala Val Asp  Gln Ala Gly 
    1820                 1825                 1830             


Ile Glu  Lys Leu Val Glu Ala  Met Ala Gln Tyr Pro  Asp Pro Gly 
    1835                 1840                 1845             


Ala Ala  Ala Ala Ala Pro Pro  Ala Ala Arg Val Pro  Asp Thr Leu 
    1850                 1855                 1860             


Met Gln  Ser Leu Ala Val Asn  Trp Arg 
    1865                 1870         


<210>  8
<211>  86
<212>  PRT
<213>  Artificial

<220>
<223>  MAGE A3 97-178

<400>  8

Leu Gly Asp Asn Gln Ile Met Pro Lys Ala Gly Leu Leu Ile Ile Val 
1               5                   10                  15      


Leu Ala Ile Ile Ala Arg Glu Gly Asp Cys Ala Pro Glu Glu Lys Ile 
            20                  25                  30          


Pro Lys Lys Leu Leu Thr Gln His Phe Val Gln Glu Asn Tyr Leu Glu 
        35                  40                  45              


Tyr Arg Gln Val Pro Gly Ser Asp Pro Ala Cys Tyr Glu Phe Leu Trp 
    50                  55                  60                  


Gly Pro Arg Ala Leu Val Glu Thr Ser Tyr Val Lys Val Leu His His 
65                  70                  75                  80  


Met Val Lys Ile Ser Gly 
                85      


<210>  9
<211>  82
<212>  PRT
<213>  Artificial

<220>
<223>  MAGE A3 190-295*

<400>  9

Thr Phe Pro Asp Leu Glu Ser Glu Phe Gln Ala Ala Leu Ser Arg Lys 
1               5                   10                  15      


Val Ala Glu Leu Val His Phe Leu Leu Leu Lys Tyr Arg Ala Arg Glu 
            20                  25                  30          


Pro Val Thr Lys Ala Glu Met Leu Gly Ser Val Val Gly Asn Trp Gln 
        35                  40                  45              


Tyr Phe Phe Pro Val Ile Phe Ser Lys Ala Ser Ser Ser Leu Gln Leu 
    50                  55                  60                  


Val Phe Gly Ile Glu Leu Met Glu Val Asp Pro Ile Gly His Leu Tyr 
65                  70                  75                  80  


Ile Phe 
        


