                         SEQUENCE LISTING

<110>  SOCPRA SCIENCES ET GENIE, S.E.C.
       Brzezinski, Ryszard
       Fortin, Melanie
       Sitouni, Mina
 
<120>  THERMOSTABLE CHITOSANASE

<130>  05202424-24PCT

<150>  US 61/437,204
<151>  2011-01-28

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  645
<212>  DNA
<213>  Paenibacillus sp. 1794

<400>  1
gggtatctaa tcctgtttgc tccccacgct ttcgcgcctc agcgtcagtt acagcccaga       60

gagtcgcctt cgccactggt gttcctccac atatctacgc atttcaccgc tacacgtgga      120

attccactct cctcttctgc actcaagtca cccagtttcc agtgcgaacc aaggttgagc      180

cttggcctta aacaccagac ttaaatgacc gcctgcgcgc gctttacgcc caataattcc      240

ggacaacgct tgccccctac gtattaccgc ggctgctggc acgtagttag ccggggcttt      300

cttctcaggt accgtcactc cgatagcagt tactctaccg gacgttcttc cctggcaaca      360

gagctttacg atccgaaaac cttcatcact cacgcggcgt tgctccgtca ggctttcgcc      420

cattgcggaa gattccctac tgctgcctcc cgtaggagtc tgggccgtgt ctcagtccca      480

gtgtggccgt tcaccctctc aggtcggcta cgcatcgtcg ccttggtgag ccgttacctc      540

accaactagc taatgcgccg caggcccatc cgcaagtgac agattgctcc gtctttcatc      600

atcccctcag gagaggaaat gagatatccg gtattagctc acgtt                      645


<210>  2
<211>  374
<212>  DNA
<213>  Paenibacillus sp. 1794

<400>  2
aacatcggtt tgatcaactc cttgtctacg ttcgcacgga tcaatgaata cggctttatc       60

gaagctccgt accgttgggt cgatccaaag acgggtgtcg taacagatca gatcgactac      120

ctgacagctg atgaggaaga caactatgtg gtcgcacagg caaacgcaaa gctgaatgaa      180

gacggcacgt ttgcggagga tatggttatc gtccgttaca acaagcagtc cgacaacatc      240

ttgccgatgc cgagcgttcg cgtggattac atggacgttt cgccgaaaca ggtcgtgtca      300

gtcgcaacgg cgctcattcc gttcctcgag aacgatgact ccgaccgcgc cctgatgggt      360

accaacatgc aacg                                                        374


<210>  3
<211>  6
<212>  PRT
<213>  Paenibacillus sp. 1794

<400>  3

Lys Trp Asn Ser Trp Lys 
1               5       


<210>  4
<211>  6
<212>  PRT
<213>  Paenibacillus sp. 1794

<400>  4

Thr Thr Asp Tyr Leu Met 
1               5       


<210>  5
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  5
aaatggaaca gctggaaa                                                     18


<210>  6
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

<400>  6
catcagatag tcggtcgt                                                     18


<210>  7
<211>  1526
<212>  DNA
<213>  Paenibacillus sp. 1794


<220>
<221>  CDS
<222>  (200)..(1432)

<400>  7
aaatatattt tacaaataat tggaaatcag gtagactaat agagaaacta atattaaagc       60

gctttaactt tcggaggggg tgggactgcg ttaatcctgt gatctgctgc ccgcgccgat      120

ggcgtcccgg gcggttacag ctgagatgaa ccgaggcata acctgtttcc taatctatga      180

aattaaagga gcgtaatgg gtg atg aaa aga aat aag aga ttt tca tta agc       232
                     Val Met Lys Arg Asn Lys Arg Phe Ser Leu Ser          
                     1               5                   10               

ggc aaa gcc gtt ctg att tgt tgt ctc gct ttt ctg atg gtt ccc gcc        280
Gly Lys Ala Val Leu Ile Cys Cys Leu Ala Phe Leu Met Val Pro Ala           
            15                  20                  25                    

gga ttt gcg ctg gcg gcg ccg aac aag cct ttc cct cag cat acg act        328
Gly Phe Ala Leu Ala Ala Pro Asn Lys Pro Phe Pro Gln His Thr Thr           
        30                  35                  40                        

tac acc agc ggc tcg atc aag ccg agt cat gta acc cag acc acg atg        376
Tyr Thr Ser Gly Ser Ile Lys Pro Ser His Val Thr Gln Thr Thr Met           
    45                  50                  55                            

gac gat gca gtc aaa tcc aaa tgg gac agc tgg aaa tca gct tac ctg        424
Asp Asp Ala Val Lys Ser Lys Trp Asp Ser Trp Lys Ser Ala Tyr Leu           
60                  65                  70                  75            

aag ccg gcc ggg acc ggc aaa tat tat gtg aag tac gat tca agc ggc        472
Lys Pro Ala Gly Thr Gly Lys Tyr Tyr Val Lys Tyr Asp Ser Ser Gly           
                80                  85                  90                

gcg acg gtg tca gaa gcg cat ggc tac ggc atg ctg ctg acc gtg ctg        520
Ala Thr Val Ser Glu Ala His Gly Tyr Gly Met Leu Leu Thr Val Leu           
            95                  100                 105                   

atg gcc gga tac gac agc agc gcg cag acc tat ttt aac gga ctg tat        568
Met Ala Gly Tyr Asp Ser Ser Ala Gln Thr Tyr Phe Asn Gly Leu Tyr           
        110                 115                 120                       

aag tac tac acc gag cat ccc agc gac aat aat ccg tac ttg atg gca        616
Lys Tyr Tyr Thr Glu His Pro Ser Asp Asn Asn Pro Tyr Leu Met Ala           
    125                 130                 135                           

tgg aaa cag aac agc agc ttt cag aat gtt gaa gga gcc gat tcc gcc        664
Trp Lys Gln Asn Ser Ser Phe Gln Asn Val Glu Gly Ala Asp Ser Ala           
140                 145                 150                 155           

act gac ggc gat atg gac atc gct tat tcg ctc ctg ttg gcg gac aag        712
Thr Asp Gly Asp Met Asp Ile Ala Tyr Ser Leu Leu Leu Ala Asp Lys           
                160                 165                 170               

cag tgg ggc agc agc ggc aca gtg aac tac ttg cag gca gcc aaa aat        760
Gln Trp Gly Ser Ser Gly Thr Val Asn Tyr Leu Gln Ala Ala Lys Asn           
            175                 180                 185                   

atc atc aat gcc att atg caa aac gac gtg aac cag acg caa tgg acc        808
Ile Ile Asn Ala Ile Met Gln Asn Asp Val Asn Gln Thr Gln Trp Thr           
        190                 195                 200                       

ctc cgg ctt ggc gat tgg gct acc gac ggt tcc tac gct acg gcg acc        856
Leu Arg Leu Gly Asp Trp Ala Thr Asp Gly Ser Tyr Ala Thr Ala Thr           
    205                 210                 215                           

cgt cct tca gac ttc atg ctg aac cat ctg aag gcg ttc cag cag gcg        904
Arg Pro Ser Asp Phe Met Leu Asn His Leu Lys Ala Phe Gln Gln Ala           
220                 225                 230                 235           

acc ggt gat acc cgg tgg act aag gtc att gat aaa acc tac tcc att        952
Thr Gly Asp Thr Arg Trp Thr Lys Val Ile Asp Lys Thr Tyr Ser Ile           
                240                 245                 250               

atc aat tcg att tat aca aat tac agt gca tcg acg gga ttg ctg ccc       1000
Ile Asn Ser Ile Tyr Thr Asn Tyr Ser Ala Ser Thr Gly Leu Leu Pro           
            255                 260                 265                   

gac ttt gtc gtc tac tca gga ggc gcg tac aag cca gcg ccg gcc gac       1048
Asp Phe Val Val Tyr Ser Gly Gly Ala Tyr Lys Pro Ala Pro Ala Asp           
        270                 275                 280                       

ttc ctg gaa gat acg acc gac ggc tcc tac gcc tat aat tcc tgc cgt       1096
Phe Leu Glu Asp Thr Thr Asp Gly Ser Tyr Ala Tyr Asn Ser Cys Arg           
    285                 290                 295                           

acg cct tgg cgg att acg acc gat tac ctg atg acc ggc gac aac cgg       1144
Thr Pro Trp Arg Ile Thr Thr Asp Tyr Leu Met Thr Gly Asp Asn Arg           
300                 305                 310                 315           

gcc ctg aat cag ctg aac cag atg aac agc tgg atc aaa ggc aag gtg       1192
Ala Leu Asn Gln Leu Asn Gln Met Asn Ser Trp Ile Lys Gly Lys Val           
                320                 325                 330               

aac agc aac ccg ggc gcc gtc aag gac gga tac aag ttg aac gga agt       1240
Asn Ser Asn Pro Gly Ala Val Lys Asp Gly Tyr Lys Leu Asn Gly Ser           
            335                 340                 345                   

gtc atc ggt tcg tat aac agc ggc gcc ttt ttc gcc ccg ttc ggc gtc       1288
Val Ile Gly Ser Tyr Asn Ser Gly Ala Phe Phe Ala Pro Phe Gly Val           
        350                 355                 360                       

agc gcc atg act tcc tcg tcg aac caa acc tgg ctg aac tcc gtg tgg       1336
Ser Ala Met Thr Ser Ser Ser Asn Gln Thr Trp Leu Asn Ser Val Trp           
    365                 370                 375                           

acg tat acg gcc gga agc tca agc gaa gga tac tat gaa gac agc atc       1384
Thr Tyr Thr Ala Gly Ser Ser Ser Glu Gly Tyr Tyr Glu Asp Ser Ile           
380                 385                 390                 395           

aag ctg ttc tcg ctc ctt gtg atg tcc ggc aac tgg tgg acg tat tga       1432
Lys Leu Phe Ser Leu Leu Val Met Ser Gly Asn Trp Trp Thr Tyr               
                400                 405                 410               

tttctgggga agcctgtcta tgggaaaata acatgaacag ccccggccgc aattgggccg     1492

gggctgtttg gctatcccca agtgatgaag tggt                                 1526


<210>  8
<211>  410
<212>  PRT
<213>  Paenibacillus sp. 1794

<400>  8

Val Met Lys Arg Asn Lys Arg Phe Ser Leu Ser Gly Lys Ala Val Leu 
1               5                   10                  15      


Ile Cys Cys Leu Ala Phe Leu Met Val Pro Ala Gly Phe Ala Leu Ala 
            20                  25                  30          


Ala Pro Asn Lys Pro Phe Pro Gln His Thr Thr Tyr Thr Ser Gly Ser 
        35                  40                  45              


Ile Lys Pro Ser His Val Thr Gln Thr Thr Met Asp Asp Ala Val Lys 
    50                  55                  60                  


Ser Lys Trp Asp Ser Trp Lys Ser Ala Tyr Leu Lys Pro Ala Gly Thr 
65                  70                  75                  80  


Gly Lys Tyr Tyr Val Lys Tyr Asp Ser Ser Gly Ala Thr Val Ser Glu 
                85                  90                  95      


Ala His Gly Tyr Gly Met Leu Leu Thr Val Leu Met Ala Gly Tyr Asp 
            100                 105                 110         


Ser Ser Ala Gln Thr Tyr Phe Asn Gly Leu Tyr Lys Tyr Tyr Thr Glu 
        115                 120                 125             


His Pro Ser Asp Asn Asn Pro Tyr Leu Met Ala Trp Lys Gln Asn Ser 
    130                 135                 140                 


Ser Phe Gln Asn Val Glu Gly Ala Asp Ser Ala Thr Asp Gly Asp Met 
145                 150                 155                 160 


Asp Ile Ala Tyr Ser Leu Leu Leu Ala Asp Lys Gln Trp Gly Ser Ser 
                165                 170                 175     


Gly Thr Val Asn Tyr Leu Gln Ala Ala Lys Asn Ile Ile Asn Ala Ile 
            180                 185                 190         


Met Gln Asn Asp Val Asn Gln Thr Gln Trp Thr Leu Arg Leu Gly Asp 
        195                 200                 205             


Trp Ala Thr Asp Gly Ser Tyr Ala Thr Ala Thr Arg Pro Ser Asp Phe 
    210                 215                 220                 


Met Leu Asn His Leu Lys Ala Phe Gln Gln Ala Thr Gly Asp Thr Arg 
225                 230                 235                 240 


Trp Thr Lys Val Ile Asp Lys Thr Tyr Ser Ile Ile Asn Ser Ile Tyr 
                245                 250                 255     


Thr Asn Tyr Ser Ala Ser Thr Gly Leu Leu Pro Asp Phe Val Val Tyr 
            260                 265                 270         


Ser Gly Gly Ala Tyr Lys Pro Ala Pro Ala Asp Phe Leu Glu Asp Thr 
        275                 280                 285             


Thr Asp Gly Ser Tyr Ala Tyr Asn Ser Cys Arg Thr Pro Trp Arg Ile 
    290                 295                 300                 


Thr Thr Asp Tyr Leu Met Thr Gly Asp Asn Arg Ala Leu Asn Gln Leu 
305                 310                 315                 320 


Asn Gln Met Asn Ser Trp Ile Lys Gly Lys Val Asn Ser Asn Pro Gly 
                325                 330                 335     


Ala Val Lys Asp Gly Tyr Lys Leu Asn Gly Ser Val Ile Gly Ser Tyr 
            340                 345                 350         


Asn Ser Gly Ala Phe Phe Ala Pro Phe Gly Val Ser Ala Met Thr Ser 
        355                 360                 365             


Ser Ser Asn Gln Thr Trp Leu Asn Ser Val Trp Thr Tyr Thr Ala Gly 
    370                 375                 380                 


Ser Ser Ser Glu Gly Tyr Tyr Glu Asp Ser Ile Lys Leu Phe Ser Leu 
385                 390                 395                 400 


Leu Val Met Ser Gly Asn Trp Trp Thr Tyr 
                405                 410 


<210>  9
<211>  378
<212>  PRT
<213>  Paenibacillus sp. 1794

<400>  9

Ala Pro Asn Lys Pro Phe Pro Gln His Thr Thr Tyr Thr Ser Gly Ser 
1               5                   10                  15      


Ile Lys Pro Ser His Val Thr Gln Thr Thr Met Asp Asp Ala Val Lys 
            20                  25                  30          


Ser Lys Trp Asp Ser Trp Lys Ser Ala Tyr Leu Lys Pro Ala Gly Thr 
        35                  40                  45              


Gly Lys Tyr Tyr Val Lys Tyr Asp Ser Ser Gly Ala Thr Val Ser Glu 
    50                  55                  60                  


Ala His Gly Tyr Gly Met Leu Leu Thr Val Leu Met Ala Gly Tyr Asp 
65                  70                  75                  80  


Ser Ser Ala Gln Thr Tyr Phe Asn Gly Leu Tyr Lys Tyr Tyr Thr Glu 
                85                  90                  95      


His Pro Ser Asp Asn Asn Pro Tyr Leu Met Ala Trp Lys Gln Asn Ser 
            100                 105                 110         


Ser Phe Gln Asn Val Glu Gly Ala Asp Ser Ala Thr Asp Gly Asp Met 
        115                 120                 125             


Asp Ile Ala Tyr Ser Leu Leu Leu Ala Asp Lys Gln Trp Gly Ser Ser 
    130                 135                 140                 


Gly Thr Val Asn Tyr Leu Gln Ala Ala Lys Asn Ile Ile Asn Ala Ile 
145                 150                 155                 160 


Met Gln Asn Asp Val Asn Gln Thr Gln Trp Thr Leu Arg Leu Gly Asp 
                165                 170                 175     


Trp Ala Thr Asp Gly Ser Tyr Ala Thr Ala Thr Arg Pro Ser Asp Phe 
            180                 185                 190         


Met Leu Asn His Leu Lys Ala Phe Gln Gln Ala Thr Gly Asp Thr Arg 
        195                 200                 205             


Trp Thr Lys Val Ile Asp Lys Thr Tyr Ser Ile Ile Asn Ser Ile Tyr 
    210                 215                 220                 


Thr Asn Tyr Ser Ala Ser Thr Gly Leu Leu Pro Asp Phe Val Val Tyr 
225                 230                 235                 240 


Ser Gly Gly Ala Tyr Lys Pro Ala Pro Ala Asp Phe Leu Glu Asp Thr 
                245                 250                 255     


Thr Asp Gly Ser Tyr Ala Tyr Asn Ser Cys Arg Thr Pro Trp Arg Ile 
            260                 265                 270         


Thr Thr Asp Tyr Leu Met Thr Gly Asp Asn Arg Ala Leu Asn Gln Leu 
        275                 280                 285             


Asn Gln Met Asn Ser Trp Ile Lys Gly Lys Val Asn Ser Asn Pro Gly 
    290                 295                 300                 


Ala Val Lys Asp Gly Tyr Lys Leu Asn Gly Ser Val Ile Gly Ser Tyr 
305                 310                 315                 320 


Asn Ser Gly Ala Phe Phe Ala Pro Phe Gly Val Ser Ala Met Thr Ser 
                325                 330                 335     


Ser Ser Asn Gln Thr Trp Leu Asn Ser Val Trp Thr Tyr Thr Ala Gly 
            340                 345                 350         


Ser Ser Ser Glu Gly Tyr Tyr Glu Asp Ser Ile Lys Leu Phe Ser Leu 
        355                 360                 365             


Leu Val Met Ser Gly Asn Trp Trp Thr Tyr 
    370                 375             


