                         SEQUENCE LISTING

<110>  FESSLER, Michael B.
       ALOOR, Jim J.
 
<120>  MODULATION OF LRCH4 ACTIVITY AND THERAPEUTIC APPLICATION THEREOF

<130>  NIHB-2556

<150>  US 61/433,491
<151>  2011-01-17

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: A3 (TRCN0000121334)

<400>  1
ccgggctctc aagtctcgga agaatctcga gattcttccg agacttgaga gctttttg         58


<210>  2
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: A5 (TRCN0000121336)

<400>  2
ccggccttct gaattaagcc ttgtactcga gtacaaggct taattcagaa ggtttttg         58


<210>  3
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Lrch4-specific siRNA

<400>  3
gtctggaaat gagtcaaca                                                    19


<210>  4
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Scrambled shRNA

<400>  4
cctaaggtta agtcgccctc gctcgagcga gggcgactta accttagg                    48


<210>  5
<211>  680
<212>  PRT
<213>  Homo Sapiens


<220>
<221>  misc_feature
<223>  Lrch4 Protein Sequence

<400>  5

Met Ala Ala Ala Val Ala Gly Pro Leu Ala Ala Gly Gly Glu Glu Ala 
1               5                   10                  15      


Ala Ala Ser Val Ser Leu Pro Gly Ser Pro Gly Leu Pro Gly Ser Arg 
            20                  25                  30          


Ser Ala Glu Arg Ala Leu Glu Glu Ala Val Ala Thr Gly Thr Leu Asn 
        35                  40                  45              


Leu Ser Asn Arg Arg Leu Lys His Phe Pro Arg Gly Ala Ala Arg Ser 
    50                  55                  60                  


Tyr Asp Leu Ser Asp Ile Thr Gln Ala Asp Leu Ser Arg Asn Arg Phe 
65                  70                  75                  80  


Pro Glu Val Pro Glu Ala Ala Cys Gln Leu Val Ser Leu Glu Gly Leu 
                85                  90                  95      


Ser Leu Tyr His Asn Cys Leu Lys Cys Leu Asn Pro Ala Leu Gly Asn 
            100                 105                 110         


Leu Thr Ala Leu Thr Tyr Leu Asn Leu Ser Arg Asn Gln Leu Ser Ser 
        115                 120                 125             


Leu Pro Pro Tyr Ile Cys Gln Leu Pro Leu Arg Val Leu Ile Ile Ser 
    130                 135                 140                 


Asn Asn Lys Leu Gly Ala Leu Pro Pro Asp Ile Ser Thr Leu Gly Ser 
145                 150                 155                 160 


Leu Arg Gln Leu Asp Val Ser Ser Asn Glu Leu Gln Ser Leu Pro Val 
                165                 170                 175     


Glu Leu Cys Ser Leu Arg Ser Leu Arg Asp Leu Asn Val Arg Arg Asn 
            180                 185                 190         


Gln Leu Ser Thr Leu Pro Asp Glu Leu Gly Asp Leu Pro Leu Val Arg 
        195                 200                 205             


Leu Asp Phe Ser Cys Asn Arg Ile Ser Arg Ile Pro Val Ser Phe Cys 
    210                 215                 220                 


Arg Leu Arg His Leu Gln Val Val Leu Leu Asp Ser Asn Pro Leu Gln 
225                 230                 235                 240 


Ser Pro Pro Ala Gln Ile Cys Leu Lys Gly Lys Leu His Ile Phe Lys 
                245                 250                 255     


Tyr Leu Thr Met Glu Ala Gly Arg Arg Gly Ala Ala Leu Gly Asp Leu 
            260                 265                 270         


Val Pro Ser Arg Pro Pro Ser Phe Ser Pro Cys Pro Ala Glu Asp Leu 
        275                 280                 285             


Phe Pro Gly Arg Arg Tyr Asp Gly Gly Leu Asp Ser Gly Phe His Ser 
    290                 295                 300                 


Val Asp Ser Gly Ser Lys Arg Trp Ser Gly Asn Glu Ser Thr Asp Asp 
305                 310                 315                 320 


Phe Ser Glu Leu Ser Phe Arg Ile Ser Glu Leu Ala Arg Asp Pro Arg 
                325                 330                 335     


Gly Pro Arg Gln Pro Arg Glu Asp Gly Ala Gly Asp Gly Asp Leu Glu 
            340                 345                 350         


Gln Ile Asp Phe Ile Asp Ser His Val Pro Gly Glu Asp Glu Asp Arg 
        355                 360                 365             


Ser Ala Ala Glu Glu Gln Leu Pro Ser Glu Leu Ser Leu Val Ala Gly 
    370                 375                 380                 


Asp Val Glu Lys Pro Ser Ser Ser Arg Arg Glu Glu Pro Ala Gly Glu 
385                 390                 395                 400 


Glu Arg Arg Arg Pro Asp Thr Leu Gln Leu Trp Gln Glu Arg Glu Arg 
                405                 410                 415     


Lys Gln Gln Gln Gln Ser Gly Gly Trp Gly Ser Pro Arg Lys Asp Ser 
            420                 425                 430         


Val Leu Lys Arg Gly Ile Arg Ala Ala Gly Ala Gly Ala Ser Ala Pro 
        435                 440                 445             


Ser Thr Gln Ala Thr Cys Asn Gly Pro Pro Lys Ser Ser Thr Thr Gln 
    450                 455                 460                 


Leu Gly Val Ser Gly Gly Gln Gly Ala Pro Thr Pro Pro Pro Thr Ser 
465                 470                 475                 480 


Gln Asp Pro Leu Pro Val Ser Gly Pro Val Thr Ala Pro Val Pro Arg 
                485                 490                 495     


Pro Leu Gly Ser Ile Gln Arg Pro Asn Ser Phe Leu Phe Arg Ser Ser 
            500                 505                 510         


Ser Gln Ser Gly Ser Ser Pro Ser Ser Pro Glu Ser Val Leu Arg Pro 
        515                 520                 525             


Arg Pro Phe Pro Gln Glu Lys Glu Leu Ile Ser Gln Leu Arg Gln Val 
    530                 535                 540                 


Leu Glu Ser Arg Leu Gln Gln Pro Leu Pro Glu Asp Leu Ala Glu Ala 
545                 550                 555                 560 


Leu Ala Asn Gly Val Leu Leu Cys Gln Leu Ala Asn Gln Leu Arg Pro 
                565                 570                 575     


Arg Ser Val Pro Phe Ile His Val Pro Ser Pro Ala Val Pro Lys Leu 
            580                 585                 590         


Ser Ala Leu Lys Ser Arg Lys Asn Val Glu Ser Phe Leu Glu Ala Cys 
        595                 600                 605             


Arg Lys Met Gly Val Pro Glu Ala Asp Leu Cys Ser Pro Ser Asp Leu 
    610                 615                 620                 


Leu Arg Gly Thr Ala Gln Gly Leu Gln Thr Val Leu Glu Ala Val Ile 
625                 630                 635                 640 


Leu Val Gly Gly Lys Ala Pro Leu Pro Val Gln Pro Ser Ser Gly Leu 
                645                 650                 655     


Gly Gly Phe Leu Leu Phe Tyr Val Val Phe Met Leu Leu Leu Tyr Val 
            660                 665                 670         


Val Tyr Thr Arg Leu Leu Gly Ser 
        675                 680 


<210>  6
<211>  2043
<212>  DNA
<213>  Homo Sapiens


<220>
<221>  misc_feature
<223>  Lrch4 Nucleotide Sequence

<400>  6
atggcggcag cagtagcggg cccactcgcc gccgggggtg aggaagctgc agcttcagtg       60

tccttgccag ggtctcctgg tctacctggg agccgtagcg cagaacgagc cctagaggag      120

gctgtggcca ccgggaccct gaacttgtcc aaccggcgtt tgaagcactt cccccggggc      180

gcggcccgca gttacgactt gtcagacatc acccaggctg acttgtctcg gaaccggttt      240

cccgaggtgc ctgaggcagc ttgccagctg gtgtccctgg aaggcctgag cctctaccac      300

aattgcctga aatgcctgaa cccagccttg gggaatctta cagccctcac ctacctcaac      360

ctcagccgga accagctgtc gtcgttgcca ccctacatct gccagctgcc ccttcgagtg      420

cttatcatca gcaacaacaa gttaggagcc ctgcctccag acatcagcac cttgggaagc      480

ctgcggcagc ttgatgtgag cagcaatgag ctgcagtccc tgcccgtgga gctgtgtagc      540

ctccgttccc tgcgggatct caatgttcga aggaaccagc tcagtaccct gcctgatgag      600

ctgggagacc ttcctctggt ccgcctggat ttctcctgta accgcatctc ccgaatcccc      660

gtctccttct gccgcctcag gcacctgcag gtcgttctgc tggatagcaa ccccctacag      720

agtccacctg cccagatatg cctgaagggg aaacttcaca tcttcaagta cctaacaatg      780

gaagctggcc ggaggggagc cgccctcggg gacctggtcc cttcccgccc cccaagtttt      840

agtccttgcc ctgccgaaga tttatttccg ggacgtcgtt atgatggtgg cctggactca      900

ggcttccaca gcgttgacag tggcagcaag aggtggtcag gaaatgagtc cacagatgat      960

ttttcagagc tgtctttccg gatctcggag ctggctcgtg atccccgggg gcctagacaa     1020

cctagggaag atggcgctgg cgatggagac ctggagcaga ttgactttat tgacagccac     1080

gttcctgggg aagatgaaga tcgaagtgca gctgaggagc agctgccttc tgaattaagc     1140

cttgtagcag gggatgtgga gaagccatct agcagcaggc gagaggagcc tgcaggggag     1200

gagaggcggc gcccagacac tttgcagttg tggcaggaac gggagcggaa gcaacagcaa     1260

cagagtgggg gatgggggtc ccccaggaag gacagcgtcc tgaagcgggg gatccgagct     1320

gccggggcag gtgcttcggc cccatccaca caggccacct gcaatggccc accaaagtcc     1380

agcactaccc aactgggagt ttcagggggg cagggagctc ccacaccacc ccccacctcc     1440

caggaccccc ttcctgtatc tggaccagtg acagctcctg ttcccaggcc cctgggctcc     1500

attcagagac caaacagctt cctcttccgg tcctcttctc agagtggctc tagtccttcc     1560

tctccagagt ctgttttgag acctcggccg tttcctcagg agaaggagct aatatcccaa     1620

cttcgccagg tcctagagtc gcggctgcag cagcccctac ctgaggacct ggcagaagct     1680

ctcgccaacg gagtcctcct ttgccagctg gcgaaccagc tgcggccccg ctctgtaccc     1740

ttcattcatg tgccctcacc tgctgtgcca aagctcagtg ctctcaagtc tcggaagaat     1800

gtcgagagtt tcctagaagc ctgtcggaaa atgggtgtgc ctgaggctga cctgtgctcg     1860

ccctcggatc tcctccgggg caccgcccag gggctgcaga ccgtcctgga ggctgtgatc     1920

ctggttgggg gcaaggcccc tctcccagtc cagccctcct ctggtctggg tggcttcctc     1980

ctcttctacg tggtcttcat gctgctgctc tatgtcgtct acactcggct cctgggctcc     2040

tag                                                                   2043

 

