                         SEQUENCE LISTING

<110>  National Research Council of Canada
       Twine, Susan
       Conlan, Joseph Wayne
 
<120>  Immunoreactive Francisella Tularensis Antigens

<130>  12258-98

<140>  61/433,385
<141>  2011-01-17

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  489
<212>  PRT
<213>  Francisella tularensis subsp. tularensis SCHU S4

<400>  1

Met Val Glu Leu Lys Val Pro Met Phe Pro Glu Ser Val Ala Asp Gly 
1               5                   10                  15      


Thr Leu Ala Gln Trp Asn Lys Asn Glu Gly Asp Phe Val Asn Glu Gly 
            20                  25                  30          


Asp Ile Leu Ala Glu Ile Glu Thr Asp Lys Val Val Leu Glu Val Pro 
        35                  40                  45              


Ala Thr Ser Ser Gly Val Leu Lys Gly Ile Lys Lys His Ala Gly Asp 
    50                  55                  60                  


Thr Val Leu Ser Glu Glu Ser Leu Ala Ile Ile Asp Thr Ala Val Ser 
65                  70                  75                  80  


Thr Ser Glu Pro Asn Gln Gln Thr Thr Asn Gln Gly Asn Ala Ser Glu 
                85                  90                  95      


Ala Thr Ala Thr Gly Gln Glu Ile Asp Ile Lys Ala Pro Val Phe Pro 
            100                 105                 110         


Glu Ser Val Ala Asp Gly Thr Ile Ser Glu Trp His Lys Lys Glu Gly 
        115                 120                 125             


Glu Ala Val Ser Glu Gly Asp Ile Leu Ala Glu Ile Glu Thr Asp Lys 
    130                 135                 140                 


Val Val Leu Glu Val Pro Ala Thr Ser Asn Gly Val Leu Thr Lys Ile 
145                 150                 155                 160 


Leu Lys Thr Ala Gly Glu Thr Val Leu Ser Ala Glu Leu Ile Ala Lys 
                165                 170                 175     


Ile Thr Ala Gly Gly Ala Thr Ala Thr Thr Lys Ser Glu Ala Ser Val 
            180                 185                 190         


Gly Val Ser Gln Ala Asn Asn Asp Pro His Leu Val Pro Ser Ala Arg 
        195                 200                 205             


Lys Ala Phe Asn Ala Ser Gly Leu Asp Thr Ala Ala Asn Ile Glu Gly 
    210                 215                 220                 


Thr Gly Lys Lys Gly Arg Ile Thr Ser Glu Asp Val Lys Lys Ala Val 
225                 230                 235                 240 


Ala Ser Val Asn Lys Pro Gln Gln Gln Thr Val Val Ile Asn Gln Gly 
                245                 250                 255     


Ala Arg Tyr Glu Lys Arg Val Lys Met Thr Arg Leu Arg Gln Thr Ile 
            260                 265                 270         


Ala Asn Arg Leu Val Glu Val Gln His Thr Asn Ala Ile Leu Thr Thr 
        275                 280                 285             


Phe Asn Glu Val Asp Met Ser Ala Val Met Glu Leu Arg Asn Lys Tyr 
    290                 295                 300                 


Lys Asp Met Phe Val Lys Glu His Asp Thr Lys Leu Gly Phe Met Ser 
305                 310                 315                 320 


Phe Phe Ile Lys Ala Ala Thr Glu Ala Leu Lys Lys Phe Pro Asp Val 
                325                 330                 335     


Asn Ala Ser Ile Asp Gly Asp Glu Ile Val Tyr His Asn Tyr Phe Asp 
            340                 345                 350         


Ile Gly Ile Ala Val Gly Thr Asp Arg Gly Leu Val Val Pro Val Leu 
        355                 360                 365             


Arg Asp Thr Asp Thr Lys Ser Leu Ala Glu Leu Glu Ala Asp Val Leu 
    370                 375                 380                 


Asp Lys Ala Ile Lys Gly Arg Asp Gly Lys Leu Ser Leu Glu Asp Met 
385                 390                 395                 400 


Gln Gly Gly Thr Phe Thr Ile Thr Asn Gly Gly Thr Tyr Gly Ser Met 
                405                 410                 415     


Leu Ser Thr Pro Ile Ile Asn Ser Pro Gln Ser Ala Ile Leu Gly Met 
            420                 425                 430         


His Asn Ile Val Glu Arg Pro Val Val Val Lys Gly Glu Ile Lys Ile 
        435                 440                 445             


Arg Pro Ile Met Tyr Leu Ala Leu Ser Tyr Asp His Arg Ile Ile Asp 
    450                 455                 460                 


Gly Gly Thr Ser Val Arg Phe Leu Lys Met Ile Lys Glu Leu Ile Glu 
465                 470                 475                 480 


Asp Pro Asn Arg Ile Leu Leu Gln Val 
                485                 


<210>  2
<211>  125
<212>  PRT
<213>  Francisella tularensis subsp. tularensis SCHU S4

<400>  2

Met Ala Ile Thr Lys Glu Asp Ile Leu Asn Ala Val Ala Glu Met Ser 
1               5                   10                  15      


Val Met Asp Val Cys Asp Leu Val Lys Met Met Glu Asp Lys Phe Gly 
            20                  25                  30          


Val Ser Ala Ala Ala Ala Val Ala Val Ala Ala Gly Pro Val Ala Gly 
        35                  40                  45              


Pro Ala Glu Ala Ala Glu Glu Lys Thr Glu Phe Asp Val Val Leu Val 
    50                  55                  60                  


Asp Ala Gly Ser Asn Lys Ile Ala Ala Ile Lys Ala Val Arg Gly Ala 
65                  70                  75                  80  


Thr Gly Leu Gly Leu Lys Glu Ala Lys Asp Ala Val Glu Gly Thr Pro 
                85                  90                  95      


Phe Thr Val Lys Glu Ala Ala Ser Lys Glu Glu Ala Glu Val Leu Lys 
            100                 105                 110         


Lys Gln Leu Glu Glu Ala Gly Ala Lys Val Glu Leu Lys 
        115                 120                 125 


<210>  3
<211>  556
<212>  PRT
<213>  Francisella tularensis subsp. tularensis SCHU S4

<400>  3

Met Ser Glu Asn Phe Lys Glu Leu Phe Glu Gln Ser Leu Lys Gln Thr 
1               5                   10                  15      


Glu Met Arg Ile Gly Lys Ile Ile Glu Ala Thr Val Val Ser Ile Asp 
            20                  25                  30          


Lys Glu Phe Ala Met Ile Asp Ala Gly Leu Lys Ser Glu Ser Phe Ile 
        35                  40                  45              


Pro Val Ser Ser Leu Lys Asn Ser Asn Gly Glu Leu Glu Val Ala Ala 
    50                  55                  60                  


Gly Asp Arg Ile Asn Val Val Leu Glu Ala Leu Asp Asn Ser Cys Gly 
65                  70                  75                  80  


Glu Thr Arg Leu Ser Arg Asp Lys Ala Lys Lys Ile Glu Leu Trp Asp 
                85                  90                  95      


Arg Ile Glu Lys Ala Phe Glu Asn Asn Glu Thr Val Leu Gly Lys Ile 
            100                 105                 110         


Thr Asn His Val Arg Gly Gly Tyr Thr Met Asp Val Glu Gly Leu Arg 
        115                 120                 125             


Ala Phe Leu Pro Gly Ser Leu Val Asp Thr Arg Pro Ile Lys Asp Val 
    130                 135                 140                 


Ala His Leu Glu Asp Lys Asp Ile Glu Leu Lys Val Val Lys Ile Asp 
145                 150                 155                 160 


Thr Lys Arg Asn Asn Ile Val Val Ser Arg Lys Ala Val Ile Glu Glu 
                165                 170                 175     


Asn Asn Ser Gly Asp Arg Asp Ala Met Leu Glu Lys Ile Ser Glu Gly 
            180                 185                 190         


Ser Val Leu Lys Gly Ile Val Lys Asn Ile Thr Asp Phe Gly Ala Phe 
        195                 200                 205             


Ile Asp Leu Gly Gly Val Asp Gly Leu Leu His Ile Thr Asp Ile Ser 
    210                 215                 220                 


Trp Ser Arg Ile Ser His Pro Thr Asp Val Leu Ser Ile Gly Gln Glu 
225                 230                 235                 240 


Ile Asp Val Lys Val Ile Lys Phe Asp Lys Glu Lys Gln Arg Ile Ser 
                245                 250                 255     


Leu Gly Ile Lys Gln Leu Gly Glu Asp Pro Trp Leu Asn Ile Ala Asn 
            260                 265                 270         


Glu Leu Pro Val Gly Ala Lys Leu Met Gly Thr Val Thr Asn Ile Thr 
        275                 280                 285             


Asp Tyr Gly Cys Phe Val Lys Leu Lys Glu Gly Ile Glu Gly Leu Val 
    290                 295                 300                 


His Thr Ser Glu Met Asp Trp Thr Asn Lys Asn Val Asn Pro His Lys 
305                 310                 315                 320 


Ala Val Ser Ile Gly Gln Glu Ile Glu Val Ile Val Leu Glu Leu Asp 
                325                 330                 335     


Ala Asp Asn His Arg Ile Ser Leu Gly Ile Lys Gln Cys Arg Pro Asn 
            340                 345                 350         


Pro Trp Ser Glu Phe Glu Lys Asn Tyr Lys Pro Gly Asp Lys Val Thr 
        355                 360                 365             


Gly Lys Ile Arg Ser Ile Thr Glu Phe Gly Val Phe Ile Gly Leu Glu 
    370                 375                 380                 


Gly Gly Ile Asp Gly Leu Val His Ile Ser Asp Val Ala Trp Asp Asn 
385                 390                 395                 400 


Pro Ala Lys Ala Ile Lys Glu Leu Lys Lys Gly Asp Glu Val Glu Ala 
                405                 410                 415     


Val Leu Val Ser Val Asn Thr Asp Leu Glu Arg Ile Ala Leu Ser Met 
            420                 425                 430         


Lys Gln Leu Ser Glu Asp Pro Phe Lys Asn Phe Ile Asn Ile His Pro 
        435                 440                 445             


Lys Gly Ser Leu Val Thr Gly Lys Val Thr Lys Val Gln Asp Asn Gly 
    450                 455                 460                 


Ala Val Val Met Leu Asp Glu Asp Asn Asn Ile Asp Gly Phe Ile Arg 
465                 470                 475                 480 


Ile Ser Glu Ile Ser Ala Glu His Thr Lys Asp Val Arg Asp Glu Leu 
                485                 490                 495     


Ser Glu Gly Gln Glu Val Glu Ala Arg Ile Ile Asn Ile Asp Ala Lys 
            500                 505                 510         


Lys Arg Ser Ile Thr Leu Ser Ile Lys Ala Val Asp Glu Asp Asn Thr 
        515                 520                 525             


Ala Ala Gly Lys Ser Asn Tyr Lys Val Glu Gln Met Thr Pro Thr Thr 
    530                 535                 540                 


Leu Gly Asp Leu Ile Lys Glu Gln Leu Asn Lys Lys 
545                 550                 555     


<210>  4
<211>  323
<212>  PRT
<213>  Francisella tularensis subsp. tularensis SCHU S4

<400>  4

Met Ser Asn Asn Asn Ser Lys Leu Glu Phe Val Pro Asn Ile Gln Leu 
1               5                   10                  15      


Lys Glu Asp Leu Gly Ala Phe Ser Tyr Lys Val Gln Leu Ser Pro Val 
            20                  25                  30          


Glu Lys Gly Met Ala His Ile Leu Gly Asn Ser Ile Arg Arg Val Leu 
        35                  40                  45              


Leu Ser Ser Leu Ser Gly Ala Ser Ile Ile Lys Val Asn Ile Ala Asn 
    50                  55                  60                  


Val Leu His Glu Tyr Ser Thr Leu Glu Asp Val Lys Glu Asp Val Val 
65                  70                  75                  80  


Glu Ile Val Ser Asn Leu Lys Lys Val Ala Ile Lys Leu Asp Thr Gly 
                85                  90                  95      


Ile Asp Arg Leu Asp Leu Glu Leu Ser Val Asn Lys Ser Gly Val Val 
            100                 105                 110         


Ser Ala Gly Asp Phe Lys Thr Thr Gln Gly Val Glu Ile Ile Asn Lys 
        115                 120                 125             


Asp Gln Pro Ile Ala Thr Leu Thr Asn Gln Arg Ala Phe Ser Leu Thr 
    130                 135                 140                 


Ala Thr Val Ser Val Gly Arg Asn Val Gly Ile Leu Ser Ala Ile Pro 
145                 150                 155                 160 


Thr Glu Leu Glu Arg Val Gly Asp Ile Ala Val Asp Ala Asp Phe Asn 
                165                 170                 175     


Pro Ile Lys Arg Val Ala Phe Glu Val Phe Asp Asn Gly Asp Ser Glu 
            180                 185                 190         


Thr Leu Glu Val Phe Val Lys Thr Asn Gly Thr Ile Glu Pro Leu Ala 
        195                 200                 205             


Ala Val Thr Lys Ala Leu Glu Tyr Phe Cys Glu Gln Ile Ser Val Phe 
    210                 215                 220                 


Val Ser Leu Arg Val Pro Ser Asn Gly Lys Thr Gly Asp Val Leu Ile 
225                 230                 235                 240 


Asp Ser Asn Ile Asp Pro Ile Leu Leu Lys Pro Ile Asp Asp Leu Glu 
                245                 250                 255     


Leu Thr Val Arg Ser Ser Asn Cys Leu Arg Ala Glu Asn Ile Lys Tyr 
            260                 265                 270         


Leu Gly Asp Leu Val Gln Tyr Ser Glu Ser Gln Leu Met Lys Ile Pro 
        275                 280                 285             


Asn Leu Gly Lys Lys Ser Leu Asn Glu Ile Lys Gln Ile Leu Ile Asp 
    290                 295                 300                 


Asn Asn Leu Ser Leu Gly Val Gln Ile Asp Asn Phe Arg Glu Leu Val 
305                 310                 315                 320 


Glu Gly Lys 
            


<210>  5
<211>  157
<212>  PRT
<213>  Francisella tularensis subsp. tularensis SCHU S4

<400>  5

Met Asp Leu Leu Lys Ala Ile Asp Arg Val Ala Glu Ile Leu Asn Ser 
1               5                   10                  15      


Ser Asp Ile Lys Glu Ile Arg Ile Lys Asp Gly Gly Ser Ser Ile Phe 
            20                  25                  30          


Met Thr Lys Asn Asn Thr Ala Ala Ile Thr Ser Val Val Phe Ala Ala 
        35                  40                  45              


Pro Val Ala Ser Asn Val Ala Ser Ala Ala Pro Ala Val Ala Thr Ala 
    50                  55                  60                  


Ala Thr Ser Ala Ala Ala Pro Lys Val Asn Leu Ala Glu Glu Ile Ser 
65                  70                  75                  80  


Gly Glu Glu Ile Lys Ser Pro Met Val Gly Thr Phe Tyr Gly Ala Ser 
                85                  90                  95      


Ser Pro Asp Ala Ala Pro Tyr Val Lys Glu Gly Gln Glu Val Lys Lys 
            100                 105                 110         


Gly Asp Val Leu Cys Ile Ile Glu Ala Met Lys Ile Met Asn Lys Ile 
        115                 120                 125             


Glu Ala Glu Arg Ala Gly Lys Ile Val Lys Ile Ile Ala Lys Asp Gly 
    130                 135                 140                 


Glu Pro Val Gln Phe Asp Gln Pro Leu Phe Ile Ile Glu 
145                 150                 155         


<210>  6
<211>  393
<212>  PRT
<213>  Francisella tularensis subsp. tularensis SCHU S4

<400>  6

Met Met Arg Leu Lys Ser Ile Val Ile Ala Thr Thr Val Leu Leu Gly 
1               5                   10                  15      


Ser Ala Thr Ala Ser Ile Ala Ala Gly Ser Asp Asn Ile Asp Thr Leu 
            20                  25                  30          


Ala Asn Thr Asn Ser Ala Thr Thr Gln Ser Ser Gly Phe Ala Ala Asn 
        35                  40                  45              


Asn Phe Ile Ala Pro Phe Ala Asn Thr Tyr Ser Ala Leu Thr Asn Lys 
    50                  55                  60                  


Asp Asn Thr Trp Gly Pro Gln Asp Arg Thr Gly Gln Trp Tyr Leu Gly 
65                  70                  75                  80  


Val Asp Ala Asn Gly Leu Ala Gly Thr Pro Asn Ser Pro Ser Gly Ala 
                85                  90                  95      


Gly Ala Asn Phe Thr Ile Gly Tyr Asn Ile Asn Lys Tyr Phe Ala Val 
            100                 105                 110         


Gln Tyr Asn Gln Leu Val Gly Arg Val Phe Ala Gly Leu Gly Glu Gly 
        115                 120                 125             


Val Val Asn Phe Ser Asn Asn Thr Met Phe Thr Pro Tyr Ala Ala Gly 
    130                 135                 140                 


Gly Ala Gly Trp Ala Asn Leu Ala Gly Gln Ala Thr Gly Ala Trp Asp 
145                 150                 155                 160 


Val Gly Gly Gly Leu Lys Phe Glu Leu Ser Arg Asn Val Gln Ala Ser 
                165                 170                 175     


Val Asp Tyr Arg Tyr Ile Gln Thr Met Ala Pro Ser Asn Ile Ser Gly 
            180                 185                 190         


Ala Asn Gly Arg Ala Gly Thr Asn Met Ile Gly Ala Gly Leu Thr Trp 
        195                 200                 205             


Phe Phe Gly Gly Lys Asp Thr Thr Asn Asn Asp Thr Gly Asn Ile Gln 
    210                 215                 220                 


Asp Asn Gly Ala Thr Thr Ala Ala Gln Thr Val Ala Met Pro Thr Ile 
225                 230                 235                 240 


Asp Glu Ser Lys Tyr Val Leu Pro Ala Gly Ile Lys Gln Cys Glu Gly 
                245                 250                 255     


Asn Phe Asn Leu Thr Glu Asp Gly Val Ala Cys Tyr Thr Ile Asn Gly 
            260                 265                 270         


Asp Asp Val Thr Val Tyr Leu Asp Thr Lys Phe Ala Tyr Asp Lys Ala 
        275                 280                 285             


Thr Leu Asn Ala Lys Gly Lys Lys Ala Ile Ala Ser Phe Val Asn Phe 
    290                 295                 300                 


Ile Lys Asp Ser Asn Ile Ser Ser Val Thr Val Lys Gly Tyr Ala Ser 
305                 310                 315                 320 


Gln Gly Gln Thr Gly Ser Glu Phe Asp Ile Tyr Asn Gln Lys Leu Ser 
                325                 330                 335     


Glu Lys Arg Ala Gln Ala Val Ala Asp Tyr Met Lys Gln Leu Gly Leu 
            340                 345                 350         


Asp Ser Glu Lys Ile Ile Thr Lys Gly Phe Gly Tyr Asn Asp Thr Leu 
        355                 360                 365             


Gly Gly Ile His Lys Ser Asp Pro Arg Asn Gln Arg Val Glu Ala Ser 
    370                 375                 380                 


Val Ser Ala Pro Leu Lys Glu Ala Asn 
385                 390             


<210>  7
<211>  741
<212>  PRT
<213>  Francisella tularensis subsp. tularensis SCHU S4

<400>  7

Met Leu Lys Lys Ile Val Thr Ala Leu Gly Met Ser Gly Met Leu Leu 
1               5                   10                  15      


Ala Ser Ser Asn Ala Ile Ala Glu Asp Thr Thr Thr Lys Asn Asp Asn 
            20                  25                  30          


Leu Ser Pro Gln Ser Val Asp Leu Ser Pro Leu Arg Asn Leu Asn Lys 
        35                  40                  45              


Leu Asp Ser Pro Met Asp Lys Asp Tyr Asn Tyr His Gln Ala Phe Lys 
    50                  55                  60                  


Lys Leu Asp Thr Glu Gln Leu Lys Lys Asp Met Gln Asp Leu Leu Thr 
65                  70                  75                  80  


Gln Ser Gln Asp Trp Trp Pro Ala Asp Phe Gly Asn Tyr Gly Pro Phe 
                85                  90                  95      


Phe Ile Arg Leu Ser Trp His Asp Ala Gly Thr Tyr Arg Ile Tyr Asp 
            100                 105                 110         


Gly Arg Gly Gly Ala Asn Arg Gly Gln Gln Arg Phe Ser Pro Leu Asn 
        115                 120                 125             


Ser Trp Pro Asp Asn Val Asn Leu Asp Lys Ala Arg Gln Leu Leu Trp 
    130                 135                 140                 


Pro Ile Lys Gln Lys Tyr Gly Asp Ala Val Ser Trp Ser Asp Leu Ile 
145                 150                 155                 160 


Val Leu Ala Gly Thr Val Ser Leu Glu Ser Met Gly Met Lys Pro Ile 
                165                 170                 175     


Gly Phe Ala Phe Gly Arg Glu Asp Asp Trp Gln Gly Asp Asp Thr Asn 
            180                 185                 190         


Trp Gly Leu Ser Pro Glu Glu Ile Met Ser Ser Asn Val Arg Asp Gly 
        195                 200                 205             


Lys Leu Ala Pro Ala Tyr Ala Ala Thr Gln Met Gly Leu Ile Tyr Val 
    210                 215                 220                 


Asn Pro Glu Gly Pro Asp Gly Lys Pro Asp Ile Lys Gly Ala Ala Ser 
225                 230                 235                 240 


Glu Ile Arg Gln Ala Phe Arg Ala Met Gly Met Thr Asp Lys Glu Thr 
                245                 250                 255     


Val Ala Leu Ile Ala Gly Gly His Thr Phe Gly Lys Thr His Gly Ala 
            260                 265                 270         


Val Pro Glu Asp Lys Val Lys Gln Ala Ile Gly Pro Ala Pro Asp Lys 
        275                 280                 285             


Ala Pro Ile Glu Gln Gln Gly Leu Gly Trp His Asn Ser Tyr Gly Thr 
    290                 295                 300                 


Gly Asn Gly Asp Asp Thr Met Gly Ser Gly Leu Glu Gly Ser Trp Thr 
305                 310                 315                 320 


Ser Thr Pro Thr Phe Trp Asn His Asp Phe Leu His Asn Leu Tyr Asn 
                325                 330                 335     


Leu Asp Trp Lys Lys Thr Leu Ser Pro Ala Gly Ala His Gln Trp Thr 
            340                 345                 350         


Pro Thr Asn Ala Lys Pro Glu Asn Met Val Pro Asp Ala His Lys Pro 
        355                 360                 365             


Gly Val Lys His Lys Pro Ile Met Phe Thr Thr Asp Leu Ala Leu Lys 
    370                 375                 380                 


Glu Asp Asp Gly Phe Asn Lys Tyr Thr Gln Glu Phe Tyr Asn Asn Pro 
385                 390                 395                 400 


Glu Glu Phe Lys Glu Glu Phe Ala Lys Ala Trp Phe Lys Leu Thr His 
                405                 410                 415     


Arg Asp Met Gly Pro Lys Ser Arg Tyr Ile Gly Pro Trp Ile Pro Glu 
            420                 425                 430         


Gln Asn Phe Ile Trp Gln Asp Pro Val Pro Ala Ala Asp Tyr Lys Gln 
        435                 440                 445             


Val Ser Thr Gln Asp Ile Ala Gln Leu Glu Gln Asp Ile Ile Asn Ser 
    450                 455                 460                 


Gly Leu Thr Asn Gln Gln Leu Ile Lys Thr Ala Trp Asp Ser Ala Ser 
465                 470                 475                 480 


Thr Tyr Arg Lys Thr Asp Tyr Arg Gly Gly Ser Asn Gly Ala Arg Ile 
                485                 490                 495     


Ala Leu Ala Pro Glu Lys Asp Trp Gln Met Asn Glu Pro Ala Lys Leu 
            500                 505                 510         


Glu Val Val Leu Thr Lys Leu Lys Glu Ile Gln Thr Asn Phe Asn Asn 
        515                 520                 525             


Ser Lys Thr Asp Gly Thr Lys Val Ser Leu Ala Asp Leu Ile Val Leu 
    530                 535                 540                 


Gly Gly Asn Val Gly Val Glu Gln Ala Ala Lys Gln Ala Gly Tyr Asn 
545                 550                 555                 560 


Ile Gln Met Pro Phe Val Pro Gly Arg Thr Asp Ala Thr Gln Ala Gln 
                565                 570                 575     


Thr Asp Ile Glu Ser Phe Asn Tyr Leu Lys Thr Lys Ser Asp Gly Phe 
            580                 585                 590         


Ile Asn Tyr Thr Asp Gly Ser Val Ser Ala Asp Lys Leu Pro Gln Thr 
        595                 600                 605             


Leu Val Glu Lys Ala Ser Met Leu Asp Leu Asn Ile Pro Glu Met Thr 
    610                 615                 620                 


Val Leu Val Gly Gly Met Arg Ala Leu Asp Val Asn Tyr Asp Asn Ser 
625                 630                 635                 640 


Gln Glu Gly Val Leu Thr Thr Thr Pro Gly Gln Leu Asn Asn Ser Phe 
                645                 650                 655     


Phe Val Asn Leu Leu Asp Met Ser Thr Gln Trp Lys Lys Ser Asp Lys 
            660                 665                 670         


Lys Asp Gly Glu Tyr Ile Gly Ile Asp Arg Lys Thr Gly Lys Gln Lys 
        675                 680                 685             


Trp Thr Ala Ser Pro Val Asp Leu Ile Phe Gly Ser Asn Ser Glu Leu 
    690                 695                 700                 


Lys Ala Val Ala Gln Val Tyr Ala Glu Asn Gly Asn Glu Gln Lys Phe 
705                 710                 715                 720 


Val Asn Asp Phe Ala Lys Ala Trp His Lys Val Met Met Leu Gly Arg 
                725                 730                 735     


Phe Asp Val Gln Gln 
            740     


<210>  8
<211>  642
<212>  PRT
<213>  Francisella tularensis subsp. tularensis SCHU S4

<400>  8

Met Gly Lys Ile Ile Gly Ile Asp Leu Gly Thr Thr Asn Ser Cys Leu 
1               5                   10                  15      


Ala Ile Met Asp Gly Lys Thr Ala Lys Val Ile Glu Asn Ala Glu Gly 
            20                  25                  30          


His Arg Thr Thr Pro Ser Val Val Ala Tyr Thr Asp Ser Gly Glu Ile 
        35                  40                  45              


Leu Val Gly Gln Ala Ala Lys Arg Gln Ala Val Thr Asn Pro Asp Asn 
    50                  55                  60                  


Thr Phe Phe Ala Ile Lys Arg Leu Ile Gly Arg Lys Tyr Asp Asp Lys 
65                  70                  75                  80  


Ala Val Gln Glu Asp Ile Lys Lys Lys Val Pro Tyr Ala Val Ile Lys 
                85                  90                  95      


Ala Asp Asn Gly Asp Ala Trp Val Ala Thr Lys Glu Gly Lys Lys Met 
            100                 105                 110         


Ala Pro Pro Gln Val Ser Ala Glu Val Leu Arg Lys Met Lys Lys Thr 
        115                 120                 125             


Ala Glu Asp Tyr Leu Gly Glu Pro Val Thr Glu Ala Val Ile Thr Val 
    130                 135                 140                 


Pro Ala Tyr Phe Asn Asp Ser Gln Arg Gln Ala Thr Lys Asp Ala Gly 
145                 150                 155                 160 


Lys Ile Ala Gly Leu Glu Val Lys Arg Ile Ile Asn Glu Pro Thr Ala 
                165                 170                 175     


Ala Ala Leu Ala Tyr Gly Val Asp Ser Lys Lys Gly Glu Gln Thr Val 
            180                 185                 190         


Ala Val Tyr Asp Leu Gly Gly Gly Thr Phe Asp Ile Ser Ile Ile Glu 
        195                 200                 205             


Ile Ala Asp Val Asp Gly Asp Asn Gln Ile Glu Val Leu Ser Thr Asn 
    210                 215                 220                 


Gly Asp Thr Phe Leu Gly Gly Glu Asp Phe Asp Leu Ala Leu Met Asn 
225                 230                 235                 240 


Tyr Leu Ile Asp Glu Phe Lys Lys Glu Gln Gly Ile Asp Leu His Asn 
                245                 250                 255     


Asp Lys Leu Ala Leu Gln Arg Val Arg Glu Ala Ala Glu Lys Ala Lys 
            260                 265                 270         


Val Glu Leu Ser Ser Ala Gln Gln Thr Asp Val Asn Leu Pro Tyr Ile 
        275                 280                 285             


Thr Ala Asp Ala Thr Gly Pro Lys His Leu Asn Ile Lys Val Thr Arg 
    290                 295                 300                 


Ala Lys Phe Glu Ser Leu Val Ser Asp Leu Val Met Arg Ser Leu Glu 
305                 310                 315                 320 


Pro Cys Lys Lys Ala Leu Glu Asp Ala Gly Leu Ser Lys Ser Asp Ile 
                325                 330                 335     


Thr Glu Val Leu Leu Val Gly Gly Gln Thr Arg Met Pro Leu Val Gln 
            340                 345                 350         


Glu Lys Val Lys Glu Phe Phe Gly Lys Glu Pro Arg Lys Asp Val Asn 
        355                 360                 365             


Pro Asp Glu Ala Val Ala Val Gly Ala Ala Ile Gln Gly Gly Val Leu 
    370                 375                 380                 


Ala Gly Asp Val Lys Asp Ile Leu Leu Leu Asp Val Thr Pro Leu Ser 
385                 390                 395                 400 


Leu Gly Ile Glu Thr Met Gly Gly Val Met Thr Lys Leu Ile Glu Arg 
                405                 410                 415     


Asn Thr Thr Ile Pro Thr Lys Lys Ser Gln Val Phe Ser Thr Ala Glu 
            420                 425                 430         


Asp Asn Gln Pro Ala Val Thr Ile His Val Leu Gln Gly Glu Arg Glu 
        435                 440                 445             


Met Ala Ser Ala Asn Lys Ser Leu Gly Arg Phe Asp Leu Ala Asp Ile 
    450                 455                 460                 


Pro Pro Ala Pro Arg Gly Met Pro Gln Ile Glu Val Thr Phe Asp Ile 
465                 470                 475                 480 


Asp Ala Asn Gly Ile Leu Asn Val Ser Ala Lys Asp Lys Ala Thr Gly 
                485                 490                 495     


Lys Glu Gln Asn Ile Val Ile Lys Ser Ser Ser Gly Leu Ser Glu Glu 
            500                 505                 510         


Asp Ile Glu Lys Met Val Gln Asp Ala Glu Ala Asn Ala Glu Ala Asp 
        515                 520                 525             


Lys Lys Phe His Asp Leu Val Thr Ala Arg Asn Thr Ala Asp Asn Leu 
    530                 535                 540                 


Ile His Ser Ser Arg Lys Ala Ile Gln Glu Leu Gly Asp Lys Val Thr 
545                 550                 555                 560 


Ala Ala Glu Lys Glu Lys Ile Glu Glu Ala Cys Lys Glu Leu Glu Ala 
                565                 570                 575     


Ala Thr Lys Gly Asp Asp Lys Gln Ala Ile Glu Ser Lys Thr Lys Ala 
            580                 585                 590         


Leu Glu Glu Ala Phe Ala Pro Ile Ala Gln Lys Ala Tyr Ala Glu Gln 
        595                 600                 605             


Ala Gln Ala Ala Val Ala Gln Gly Gly Ala Lys Ala Glu Glu Pro Lys 
    610                 615                 620                 


Lys Glu Glu Asp Val Val Asp Ala Asp Phe Glu Asp Val Glu Asp Asp 
625                 630                 635                 640 


Lys Lys 
        


<210>  9
<211>  631
<212>  PRT
<213>  Francisella tularensis subsp. tularensis SCHU S4

<400>  9

Met Ser Ile Glu Ile Val Lys Val Pro Asp Ile Gly Asp Tyr Asp Asn 
1               5                   10                  15      


Val Asp Val Ile Glu Val Asn Val Ala Val Gly Asp Val Ile Ala Glu 
            20                  25                  30          


Glu Asp Ser Leu Ile Thr Leu Glu Thr Asp Lys Ala Ser Met Glu Val 
        35                  40                  45              


Pro Ser Pro Phe Ala Gly Lys Ile Thr Lys Leu Thr Val Lys Val Gly 
    50                  55                  60                  


Asp Lys Val Ser Gln Gly Thr Ala Ile Met Glu Val Glu Val Glu Ser 
65                  70                  75                  80  


Ala Ala Asp Gln Ala Ala Thr Thr Gln Ser Gln Pro Gln Thr Thr Ser 
                85                  90                  95      


Ser Ala Pro Val Ala Ala Thr Thr Asn Leu Ile Val Asp Val Glu Val 
            100                 105                 110         


Pro Asp Ile Gly Asp Tyr Asp Ser Val Asp Val Ile Glu Val Ser Val 
        115                 120                 125             


Lys Val Gly Asp Glu Ile Ala Glu Glu Asp Ser Leu Ile Thr Leu Glu 
    130                 135                 140                 


Thr Tyr Lys Ala Ser Met Glu Val Pro Ser Pro Val Ala Gly Lys Val 
145                 150                 155                 160 


Val Glu Val Ile Thr Lys Val Gly Asp Lys Val Ser Gln Gly Ser Leu 
                165                 170                 175     


Ile Leu Lys Val Glu Thr Gly Ser Ser Ala Gln Ala Pro Ala Gln Glu 
            180                 185                 190         


Gln Ser Gln Gln Ser Ala Pro Val Lys Ser Ala Ala Glu Glu Ile Ile 
        195                 200                 205             


Asp Val Lys Val Pro Asp Ile Gly Asp Tyr Asp Ser Val Asp Val Ile 
    210                 215                 220                 


Glu Val Ser Val Ala Val Gly Asp Lys Ile Glu Glu Glu Asp Ser Leu 
225                 230                 235                 240 


Ile Thr Leu Glu Thr Asp Lys Ala Ser Met Glu Val Pro Ser Pro Val 
                245                 250                 255     


Ala Gly Glu Val Val Glu Ile Ile Thr Lys Val Gly Asp Lys Val Ser 
            260                 265                 270         


Gln Gly Ser Leu Ile Leu Lys Val Lys Thr Gln Gly Ser Ala Pro Val 
        275                 280                 285             


Glu Gln Thr Ser Ser Gln Pro Ala Pro Ala Lys Gln Glu Gln Ala Lys 
    290                 295                 300                 


Gln Gln Ala Ala Thr Pro Ala Ala Pro Thr Pro Ala Ser Ser Ser Val 
305                 310                 315                 320 


Asn Glu Tyr Ala Val Asp Asn Ser Asn Ala His Ala Ser Pro Ala Val 
                325                 330                 335     


Arg Lys Leu Ala Arg Ile Leu Asn Ile Asp Leu Ser Lys Val Lys Ala 
            340                 345                 350         


Thr Gly Arg Lys Gly Arg Val Thr Lys Glu Asp Cys Tyr Asn Tyr Ile 
        355                 360                 365             


Lys His Ala Val Thr Gln Val Gln Thr Gly Lys Val Ala Ala Ser Gly 
    370                 375                 380                 


Ser Gly Leu Asp Leu Leu Asp Asp Pro Val Val Asp Phe Ala Lys Phe 
385                 390                 395                 400 


Gly Glu Ile Glu Thr Gln Pro Leu Ser Arg Ile Asn Lys Ile Ser Ala 
                405                 410                 415     


Lys Asn Leu His Arg Asn Trp Val Lys Ile Pro His Val Thr Phe Tyr 
            420                 425                 430         


Asp Asp Ala Asp Val Thr Asp Leu Glu Glu Phe Arg Asn Ala Lys Lys 
        435                 440                 445             


Ala Phe Ala Glu Lys Lys Gly Ile Lys Ile Thr Pro Leu Ser Phe Leu 
    450                 455                 460                 


Val Lys Ala Ala Ala Val Ala Leu Gln Glu Phe Pro Arg Phe Asn Ser 
465                 470                 475                 480 


Ser Leu Ser Asn Asp Gly Glu Asn Leu Ile Ile Lys Lys Tyr Tyr Asn 
                485                 490                 495     


Ile Gly Phe Ala Ala Asp Thr Pro Ala Gly Leu Met Val Pro Val Val 
            500                 505                 510         


Lys Asp Ala Asp Lys Lys Gly Ile Ile Glu Ile Ser Lys Asp Ile Met 
        515                 520                 525             


Glu Leu Ala Gly Lys Ala Arg Asp Gly Lys Leu Gly Ala Lys Asp Met 
    530                 535                 540                 


Thr Gly Ala Thr Phe Thr Ile Ser Ser Leu Gly Val Leu Gly Thr Thr 
545                 550                 555                 560 


Ser Phe Thr Pro Ile Ile Asn Met Pro Glu Val Ala Ile Met Gly Val 
                565                 570                 575     


Ser Lys Thr Ala Val Lys Pro Ile Trp Asn Gly Lys Glu Phe Ile Pro 
            580                 585                 590         


Arg Thr Met Leu Pro Leu Ser Leu Ser Thr Asp His Arg Val Ile Asp 
        595                 600                 605             


Gly Ala Leu Ala Ala Lys Phe Leu Thr Arg Tyr Cys Gln Ile Leu Ser 
    610                 615                 620                 


Asp Leu Arg Glu Ile Ile Met 
625                 630     


<210>  10
<211>  544
<212>  PRT
<213>  Francisella tularensis subsp. tularensis SCHU S4

<400>  10

Met Ala Ala Lys Gln Val Leu Phe Ser Asp Glu Ala Arg Ala Lys Met 
1               5                   10                  15      


Leu Asp Gly Val Asn Thr Leu Ala Asn Ala Val Lys Val Thr Leu Gly 
            20                  25                  30          


Pro Lys Gly Arg Asn Val Val Leu Asp Lys Ser Phe Gly Thr Pro Thr 
        35                  40                  45              


Ile Thr Lys Asp Gly Val Ser Val Ala Lys Glu Ile Glu Leu Glu Asp 
    50                  55                  60                  


Lys Phe Glu Asn Met Gly Ala Gln Ile Val Lys Glu Val Ala Ser Lys 
65                  70                  75                  80  


Thr Ala Asp Val Ala Gly Asp Gly Thr Thr Thr Ala Thr Val Leu Ala 
                85                  90                  95      


Gln Ala Leu Leu Thr Glu Gly Leu Lys Ala Val Ala Ala Gly Met Asn 
            100                 105                 110         


Pro Met Asp Leu Lys Arg Gly Ile Asp Lys Ala Thr Ala Arg Leu Val 
        115                 120                 125             


Glu Glu Leu Lys Ala Leu Ser Lys Pro Cys Ser Asp Pro Lys Ser Ile 
    130                 135                 140                 


Glu Gln Val Gly Thr Ile Ser Ala Asn Ser Asp Ala Thr Val Gly Lys 
145                 150                 155                 160 


Leu Ile Ala Asp Ala Met Ala Lys Val Gly Lys Glu Gly Val Ile Thr 
                165                 170                 175     


Val Glu Glu Gly Lys Gly Phe Glu Asp Glu Leu Asp Val Val Glu Gly 
            180                 185                 190         


Met Gln Phe Asp Arg Gly Tyr Leu Ser Pro Tyr Phe Ala Thr Asn Gln 
        195                 200                 205             


Glu Asn Met Thr Thr Asp Leu Glu Asn Pro Tyr Ile Leu Ile Val Asp 
    210                 215                 220                 


Lys Lys Ile Ser Asn Ile Arg Asp Leu Leu Pro Ile Leu Glu Gly Val 
225                 230                 235                 240 


Ser Lys Ser Gly Arg Ala Leu Leu Ile Ile Ala Glu Asp Val Glu Ser 
                245                 250                 255     


Glu Ala Leu Ala Thr Leu Val Val Asn Asn Met Arg Gly Val Val Lys 
            260                 265                 270         


Val Cys Ala Val Lys Ala Pro Gly Phe Gly Asp Arg Arg Lys Ala Met 
        275                 280                 285             


Leu Glu Asp Ile Ala Thr Leu Thr Gly Ala Thr Phe Val Ser Glu Asp 
    290                 295                 300                 


Leu Ser Met Lys Leu Glu Glu Thr Asn Met Glu His Leu Gly Thr Ala 
305                 310                 315                 320 


Ser Arg Val Gln Val Thr Lys Asp Asn Thr Thr Ile Ile Asp Gly Ala 
                325                 330                 335     


Gly Glu Lys Glu Ala Ile Ala Lys Arg Ile Asn Val Ile Lys Ala Asn 
            340                 345                 350         


Ile Ala Glu Ala Asn Ser Asp Tyr Asp Arg Glu Lys Leu Gln Glu Arg 
        355                 360                 365             


Leu Ala Lys Leu Ser Gly Gly Val Ala Val Ile Lys Val Gly Ala Val 
    370                 375                 380                 


Thr Glu Ala Glu Met Lys Glu Lys Lys Asp Arg Val Asp Asp Ala Leu 
385                 390                 395                 400 


His Ala Thr Arg Ala Ala Val Glu Glu Gly Ile Val Ala Gly Gly Gly 
                405                 410                 415     


Val Ala Leu Ile Arg Ala Gln Lys Ala Leu Asp Gly Leu Thr Gly Glu 
            420                 425                 430         


Asn Asp Asp Gln Asn Tyr Gly Ile Ala Leu Leu Arg Lys Ala Ile Glu 
        435                 440                 445             


Ala Pro Leu Arg Gln Ile Val Ser Asn Ala Gly Gly Glu Ser Ser Val 
    450                 455                 460                 


Val Val Asn Gln Val Lys Ala Asn Gln Gly Asn Tyr Gly Tyr Asn Ala 
465                 470                 475                 480 


Ala Asn Asp Thr Tyr Gly Asp Met Val Glu Met Gly Ile Leu Asp Pro 
                485                 490                 495     


Thr Lys Val Thr Arg Ser Ala Leu Gln His Ala Ala Ser Ile Ala Gly 
            500                 505                 510         


Leu Met Ile Thr Thr Glu Ala Met Ile Gly Glu Ile Lys Glu Ala Ala 
        515                 520                 525             


Pro Ala Met Pro Met Gly Gly Gly Met Gly Gly Met Pro Gly Met Met 
    530                 535                 540                 


<210>  11
<211>  130
<212>  PRT
<213>  Francisella tularensis subsp. tularensis SCHU S4

<400>  11

Met Lys Leu Arg Lys Val Leu Ile Ala Thr Leu Leu Gly Ala Ser Ala 
1               5                   10                  15      


Leu Ser Leu Ser Ser Cys Trp Leu Leu Val Gly Ala Ala Val Gly Gly 
            20                  25                  30          


Gly Thr Ala Ala Tyr Ile Ser Gly Glu Tyr Ser Met Asn Met Ser Gly 
        35                  40                  45              


Ser Val Lys Asp Ile Tyr Asn Ala Thr Leu Lys Ala Val Gln Ser Asn 
    50                  55                  60                  


Asp Asp Phe Val Ile Thr Lys Lys Ser Ile Thr Ser Val Asp Ala Val 
65                  70                  75                  80  


Val Asp Gly Ser Ile Lys Val Asp Ser Thr Ser Phe Tyr Val Lys Ile 
                85                  90                  95      


Glu Lys Leu Thr Asp Asn Ala Ser Lys Val Thr Ile Lys Phe Gly Thr 
            100                 105                 110         


Phe Gly Asp Gln Ala Met Ser Ala Thr Leu Met Asp Gln Ile Gln Lys 
        115                 120                 125             


Asn Leu 
    130 


