                         SEQUENCE LISTING

<110>  F. Hoffmann - La Roche AG
 
<120>  A pharmaceutical composition of  a complex of an anti-DIG 
       antibody and digoxigenin that is conjugated to a peptide

<130>  27209 WO

<150>  EP11150037
<151>  2011-01-03

<160>  39    

<170>  PatentIn version 3.5

<210>  1
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide

<400>  1

Ile Lys Pro Glu Ala Pro Gly Glu Asp Ala Ser Pro Glu Glu Leu Asn 
1               5                   10                  15      


Arg Tyr Tyr Ala Ser Leu Arg His Tyr Leu Asn Leu Val Thr Arg Gln 
            20                  25                  30          


Arg Tyr 
        


<210>  2
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<400>  2

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  3
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<400>  3

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  4
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  m-Tyr

<400>  4

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  5
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  3-iodo-Tyr

<400>  5

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  6
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  3,5 di F-Tyr

<400>  6

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  7
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  2,6 di F-Tyr

<400>  7

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  8
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  2,6 di Me-Tyr

<400>  8

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  9
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  4-methoxy-Phe

<400>  9

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Phe 
1               5                   10                  15  


<210>  10
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<400>  10

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Phe 
1               5                   10                  15  


<210>  11
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  4-amino-Phe

<400>  11

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Phe 
1               5                   10                  15  


<210>  12
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  4 F-Phe

<400>  12

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Phe 
1               5                   10                  15  


<210>  13
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  4(CH2OH)-Phe

<400>  13

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Phe 
1               5                   10                  15  


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  4-trifluoro methyl-Phe

<400>  14

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Phe 
1               5                   10                  15  


<210>  15
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  3 F-Phe

<400>  15

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Phe 
1               5                   10                  15  


<210>  16
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  2,3,4,5,6 Penta F-Phe

<400>  16

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Phe 
1               5                   10                  15  


<210>  17
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  3,4 dichloro-Phe

<400>  17

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Phe 
1               5                   10                  15  


<210>  18
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  Cha

<400>  18

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Xaa 
1               5                   10                  15  


<210>  19
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<400>  19

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Trp 
1               5                   10                  15  


<210>  20
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  1-Nal

<400>  20

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Xaa 
1               5                   10                  15  


<210>  21
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  2-Nal

<400>  21

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Xaa 
1               5                   10                  15  


<210>  22
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  (C-alpha-methyl)-Tyr

<400>  22

Ile Lys Xaa Arg His Tyr Leu Asn Leu Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  23
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<400>  23

Ile Lys Xaa Arg His Tyr Leu Asn Trp Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  24
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Nle

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<400>  24

Ile Xaa Xaa Arg His Tyr Leu Asn Trp Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  25
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Ac-Ile

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  2,6 difluoro-Tyr

<400>  25

Ile Lys Xaa Arg His Tyr Leu Asn Trp Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  26
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Ac-Ile

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<400>  26

Ile Lys Xaa Arg His Tyr Leu Asn Trp Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  27
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Pentoyl-Ile

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<400>  27

Ile Lys Xaa Arg His Tyr Leu Asn Trp Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  28
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Trimethylacetyl-Ile

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<400>  28

Ile Lys Xaa Arg His Tyr Leu Asn Trp Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  29
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Cyclohexylacetyl-Ile

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<400>  29

Ile Lys Xaa Arg His Tyr Leu Asn Trp Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  30
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Benzoyl-Ile

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<400>  30

Ile Lys Xaa Arg His Tyr Leu Asn Trp Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  31
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Adamantoyl-Ile

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Pqa

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  (NMe)Arg

<400>  31

Ile Lys Xaa Arg His Tyr Leu Asn Trp Val Thr Arg Gln Arg Tyr 
1               5                   10                  15  


<210>  32
<211>  26
<212>  PRT
<213>  Homo sapiens

<400>  32

Gly Ile Gly Ala Val Leu Lys Val Leu Thr Thr Gly Leu Pro Ala Leu 
1               5                   10                  15      


Ile Ser Trp Ile Lys Arg Lys Arg Gln Gln 
            20                  25      


<210>  33
<211>  39
<212>  PRT
<213>  Homo sapiens

<400>  33

Phe Ala Leu Leu Gly Asp Phe Phe Arg Lys Ser Lys Glu Lys Ile Gly 
1               5                   10                  15      


Lys Glu Phe Lys Arg Ile Val Gln Arg Ile Lys Asp Phe Leu Arg Asn 
            20                  25                  30          


Leu Val Pro Arg Thr Glu Ser 
        35                  


<210>  34
<211>  39
<212>  PRT
<213>  Homo sapiens

<400>  34

Asn Lys Arg Phe Ala Leu Leu Gly Asp Phe Phe Arg Lys Ser Lys Glu 
1               5                   10                  15      


Lys Ile Gly Lys Glu Phe Lys Arg Ile Val Gln Arg Ile Lys Asp Phe 
            20                  25                  30          


Leu Arg Asn Leu Val Pro Arg 
        35                  


<210>  35
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  35

Gln His Arg Tyr Gln Gln Leu Gly Ala Gly Leu Lys Val Leu Phe Lys 
1               5                   10                  15      


Lys Thr His Arg Ile Leu Arg Arg Leu Phe Asn Leu Ala Lys 
            20                  25                  30  


<210>  36
<211>  107
<212>  PRT
<213>  Mus musculus

<400>  36

Asp Val Gln Met Thr Gln Ser Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Lys Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ser Ser Thr Leu Leu Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Arg Gly Ser Gly Thr Asp Phe Ser Leu Thr Ile Thr Asn Leu Glu Arg 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Ser Ile Thr Leu Pro Pro 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  37
<211>  125
<212>  PRT
<213>  Mus musculus

<400>  37

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Val Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          


Ala Met Ser Trp Ile Arg Gln Thr Pro Glu Asn Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Ser Ile Asn Ile Gly Ala Thr Tyr Ala Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Phe 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Gly Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Ser Pro Tyr Glu Tyr Asp Lys Ala Tyr Tyr Ser Met 
            100                 105                 110         


Ala Tyr Trp Gly Pro Gly Thr Ser Val Thr Val Ser Ser 
        115                 120                 125 


<210>  38
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  humanized <dig> VL

<400>  38

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Lys Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ser Ser Thr Leu Leu Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Ile Thr Leu Pro Pro 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105             


<210>  39
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized <dig> VH

<400>  39

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          


Ala Met Ser Trp Ile Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ser Ile Asn Ile Gly Ala Thr Tyr Ile Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Ser Pro Tyr Glu Tyr Asp Lys Ala Tyr Tyr Ser Met 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120                 125 


