                         SEQUENCE LISTING

<110>  Intervet Int. BV
 
<120>  Canine babesiosis vaccine antigen

<130>  2010-015

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  PRT
<213>  Babesia rossi

<400>  1

Met Leu Leu Ser Asn Val Ser Phe Pro Gln Pro Val Ser Ser Val Lys 
1               5                   10                  15      


Leu Leu Glu Glu Tyr 
            20      


<210>  2
<211>  21
<212>  PRT
<213>  Babesia canis

<400>  2

Ile Leu Leu Ser Asn Val Glu Phe His Thr Pro Val Ser Ser Val Lys 
1               5                   10                  15      


Leu Leu Lys Glu Tyr 
            20      


<210>  3
<211>  19
<212>  PRT
<213>  Babesia rossi

<400>  3

Val Leu Met Val Leu Thr Lys Cys Asn Leu Lys Met His Val Thr Glu 
1               5                   10                  15      


Glu Gln Leu 
            


<210>  4
<211>  19
<212>  PRT
<213>  Babesia rossi

<400>  4

Phe Leu Met Val Leu Thr Lys Cys Asp Leu Met Met Ala Ile Pro Glu 
1               5                   10                  15      


Glu Gln Leu 
            


<210>  5
<211>  19
<212>  PRT
<213>  Babesia canis

<400>  5

Val Leu Met Ile Leu Thr Lys Cys Asp Leu Lys Ala Glu Val Thr Glu 
1               5                   10                  15      


Glu Gln Ile 
            


<210>  6
<211>  321
<212>  PRT
<213>  Babesia canis

<400>  6

Met Met Leu Leu Phe Ala Leu Ser Thr Leu Val Thr Phe Ala Phe Cys 
1               5                   10                  15      


Asp Gly Glu Asn Thr Ile Leu Leu Ser Asn Val Glu Phe His Thr Pro 
            20                  25                  30          


Val Ser Ser Val Lys Leu Leu Lys Glu Tyr Ser Ser Asn Gln Glu Ser 
        35                  40                  45              


Met Ala Val Ile Met Met Leu Thr Glu Met Pro Asn Thr Ser Gly Lys 
    50                  55                  60                  


Leu Thr Asp Gly Lys Val His Val Ala Asn Asp Asn Val Lys Cys Ala 
65                  70                  75                  80  


Asp Leu Ala Leu Ala Tyr Gln Glu Leu Lys Lys Ala Gly Lys Val Thr 
                85                  90                  95      


Ser Trp Ser Pro Thr Asp Asp Asn Asp Lys Val Val Pro His Gly Ile 
            100                 105                 110         


Trp Phe Ile Glu Gly Val Tyr Glu Thr Asp Lys Met Phe Glu Val Tyr 
        115                 120                 125             


Lys Thr Leu Thr Asp Pro Glu Asp Pro Ser Glu Val Thr Arg Leu Thr 
    130                 135                 140                 


Thr Val Ser Gly Ala Ser Gly Ser Ala Gln Ser Gln Pro Ala Gly Thr 
145                 150                 155                 160 


Thr Asp Gly Val Ser Gly Ser Ala Ala Ser Ala Ser Gly Ser Ser Gly 
                165                 170                 175     


Ser Thr Thr Ser His Ser Thr Thr Ala Thr Thr Ser Ser Thr Ser Thr 
            180                 185                 190         


Val Ser Thr Ser Ser Ser Gly Ala Ser Thr Ser Ser Ser Thr Asp Gln 
        195                 200                 205             


Ala Ser Met Leu Thr Thr Gln Thr Ser Tyr Ser Ala Gly Ser Ser Val 
    210                 215                 220                 


His Lys Ser Ala Val Val Ala Pro Thr Gln Ser Thr Thr Pro Asp Asn 
225                 230                 235                 240 


Ala Glu Ser Gly Ala Lys Gln Ser Lys Ala Ala Val Gln Glu Pro Lys 
                245                 250                 255     


Asn Val Leu Met Ile Leu Thr Lys Cys Asp Leu Lys Ala Glu Val Thr 
            260                 265                 270         


Glu Glu Gln Ile Arg Ser Gln Gly Asn Pro Glu Ser Asn Gly Ser Ser 
        275                 280                 285             


Ser Glu Pro Thr Ala Ala Ser Pro Lys Leu Thr Thr Ala Ala Ser Gly 
    290                 295                 300                 


Phe Thr Ala Ala Ile Thr Pro Leu Phe Met Val Pro Leu Met Phe Phe 
305                 310                 315                 320 


Ala 
    


<210>  7
<211>  278
<212>  PRT
<213>  Babesia rossi

<400>  7

Met Met Leu Leu Phe Ala Phe Ser Ser Leu Leu Ala Val Ala Ser Cys 
1               5                   10                  15      


Thr Asp Phe Asn Thr Met Leu Leu Ser Asn Val Ser Phe Pro Gln Pro 
            20                  25                  30          


Val Ser Ser Val Lys Leu Leu Glu Glu Tyr Ala Lys Tyr Gln Lys Gly 
        35                  40                  45              


Tyr Val Leu Tyr Phe Gln Met Arg Asp Asp Leu Pro Lys Lys Glu Asp 
    50                  55                  60                  


Thr Glu Arg Phe Ser Gln Tyr Val Gln Leu Glu Asn Ala Glu Cys Thr 
65                  70                  75                  80  


Asp Leu Ala Ala Ile Tyr Asn Ile Met Gln Arg Glu Gly Arg Ile Phe 
                85                  90                  95      


Glu Tyr Lys His Glu Ser Glu Leu Pro Asp Tyr Pro Glu Gly Leu Trp 
            100                 105                 110         


Leu Leu Asp Ala Gln Asp Asp Lys Glu Glu Ile Leu His Thr Phe Glu 
        115                 120                 125             


Thr Thr Leu Pro Pro Thr Ser Thr Asn Gly Asn Glu His Ala Asp Lys 
    130                 135                 140                 


Thr Arg Glu Ala Pro Lys Pro Arg Pro Asp Ala Pro Ala Ala Ser Asp 
145                 150                 155                 160 


Thr Gln Arg Ala Gln Asp Asn Gln Glu Lys Thr Pro Thr Glu Ser Ser 
                165                 170                 175     


Thr Gly Ser Arg Asp Thr Val Gln Pro Gln Thr Ala Pro Ala Thr Ser 
            180                 185                 190         


Asn Ala Val Thr Gly Thr Ser Ser Thr Thr Ser Ser Val Gln Ser Gln 
        195                 200                 205             


Ala Val Leu Gly Asn Ser Glu Ala Thr Thr Gly Thr Gln Gln Ser Ala 
    210                 215                 220                 


Glu Asn Val Lys Val Leu Met Val Leu Thr Lys Cys Asn Leu Lys Met 
225                 230                 235                 240 


His Val Thr Glu Glu Gln Leu Ser Lys His Ser Asn Ile Pro Arg Lys 
                245                 250                 255     


His Gly Ser Gly Phe Thr Pro Ala Ile Ala Phe Thr Ser Leu Leu Pro 
            260                 265                 270         


Phe Leu Leu Met Met Ser 
        275             


<210>  8
<211>  274
<212>  PRT
<213>  Babesia rossi

<400>  8

Met Met Leu Leu Phe Ala Phe Ser Ser Leu Leu Ala Val Ala Ser Cys 
1               5                   10                  15      


Thr Asp Phe Asn Thr Met Leu Leu Ser Asn Val Ser Phe Pro Gln Pro 
            20                  25                  30          


Val Ser Ser Val Lys Leu Leu Glu Glu Tyr Ala Lys Asp Asp Lys Val 
        35                  40                  45              


Thr Val Ile Phe Ser Val Leu Asp Thr Leu Pro Thr Asp Glu Ala Asn 
    50                  55                  60                  


Gly Thr Arg Tyr Ser Lys Tyr Lys Ala Asp Asp Asn Asp Lys Cys Val 
65                  70                  75                  80  


Asp Leu Ala Leu Val Phe Gln Glu Leu Gln Lys Lys Gly Leu Val Lys 
                85                  90                  95      


Glu Phe Gly Asn Glu Asp Thr Pro Pro Glu Glu Val Asn Gly Leu Trp 
            100                 105                 110         


Leu Met Arg Gly Lys His Ala Gly His Asn Met Leu Glu Val Phe Glu 
        115                 120                 125             


Thr Leu Val Asn Pro Arg Arg Gly Leu Glu Asp Leu Pro Val Thr Pro 
    130                 135                 140                 


Lys Ser Ala Glu Gly Arg Arg Glu Ala Val Ser Arg Ser Thr Ala Glu 
145                 150                 155                 160 


Asn Gln Ala Gly Thr Asn Gly Glu Ser Gly Ser Ser Gln Thr Ala Ser 
                165                 170                 175     


Asn Ala Gly Thr Ser Gln Val Thr Ala Ala Arg Val Ser Gly Val Gln 
            180                 185                 190         


Ala Gln Ser Val Ala Ala Gly Thr Asn Gly Pro Gln Asn Val Arg Glu 
        195                 200                 205             


Asp Thr Val Pro Ala Pro Glu Ala Val Pro Ser Asn Gln Asp Met Lys 
    210                 215                 220                 


Phe Leu Met Val Leu Thr Lys Cys Asp Leu Met Met Ala Ile Pro Glu 
225                 230                 235                 240 


Glu Gln Leu Ser Gly Pro Lys Asn Met Arg Glu Asn Gln Glu Ser Gly 
                245                 250                 255     


Phe Thr Pro Ala Ile Ala Phe Thr Ser Leu Leu Pro Phe Leu Leu Met 
            260                 265                 270         


Met Ser 
        


<210>  9
<211>  966
<212>  DNA
<213>  Babesia canis


<220>
<221>  mRNA
<222>  (1)..(966)

<400>  9
atgatgctgc tcttcgcctt gtctactctt gtcaccttcg ccttctgcga tggtgaaaac       60

actatacttt tatccaatgt agaattccat actccagtat ccagtgtaaa gctgcttaaa      120

gaatacagta gcaatcagga atcaatggcc gttattatga tgctaaccga aatgccaaac      180

acatcaggaa agcttaccga tggcaaagtt catgtggcca atgataacgt taaatgtgct      240

gatttggctc tggcttatca agaactcaaa aaggccggca aggtgacatc atggagtcca      300

actgatgaca acgataaggt ggtacctcat ggaatctggt tcatagaggg cgtctatgag      360

actgataaga tgttcgaggt ttacaagacc ctgaccgatc ctgaggaccc aagcgaagtt      420

actcgcttga caactgtttc tggtgcttcc ggatctgctc aaagccagcc tgctggtact      480

actgatggtg tctcgggtag cgctgctagt gcctctggtt cctctggttc cactacttcc      540

cattctacta ccgctactac cagctccact agtaccgttt ctacttcctc ttctggggct      600

tctacttcta gttctactga ccaggcatcc atgcttacca cccaaacatc ctacagtgca      660

ggatctagcg ttcataaaag cgctgtggtt gctcccactc agagtaccac tcctgataat      720

gctgaatctg gcgcaaagca aagcaaagct gcggttcagg aacctaagaa tgttttgatg      780

attctgacca agtgtgatct taaggccgaa gttaccgagg aacagataag aagccaagga      840

aacccagaaa gcaatgggtc ttcttctgaa cccactgctg cttctcctaa acttactacc      900

gctgcttctg gattcactgc cgccattacc cccttgttca tggtcccact catgtttttc      960

gcctaa                                                                 966


<210>  10
<211>  837
<212>  DNA
<213>  Babesia rossi


<220>
<221>  mRNA
<222>  (1)..(837)

<400>  10
atgatgttac tattcgcttt ctcttctctt ctcgcagtgg cctcgtgcac tgattttaac       60

accatgttat tgtccaatgt gagctttcct cagccggttt cgagtgtaaa actacttgaa      120

gaatatgcta aatatcagaa gggttatgtg ttgtatttcc aaatgcgtga tgatttgcca      180

aagaaagagg atactgagag gtttagtcaa tatgttcaac tggagaacgc cgaatgtact      240

gacttggctg ctatctataa tattatgcaa cgtgagggta gaatatttga atacaagcat      300

gaaagcgaac ttcctgatta tcctgaaggc ctgtggttgt tggatgccca ggatgataag      360

gaagaaattc tccacacctt tgagactaca ctccccccta cctctacaaa tggtaatgaa      420

cacgctgata agactagaga ggcacctaag ccaagaccag atgctccagc tgcttctgat      480

acccaacgtg cccaagacaa tcaggaaaag acacccactg agtcgtcaac aggttctcga      540

gacactgttc aacctcaaac tgcccctgca acttcaaacg cagtaactgg tacgtcatca      600

acgacatcaa gtgtgcagag ccaagctgta ttagggaact ctgaagctac tacagggact      660

cagcaatcag ctgaaaatgt taaggtttta atggttctga cgaagtgcaa ccttaaaatg      720

catgttactg aggaacagtt gagcaagcac agcaacatac cccgtaagca tggatcagga      780

ttcactccgg ctattgcatt tacctctctt ctaccattcc tcttgatgat gtcctaa         837


<210>  11
<211>  825
<212>  DNA
<213>  Babesia rossi


<220>
<221>  mRNA
<222>  (1)..(825)

<400>  11
atgatgttac tattcgcttt ctcttctctt ctcgcagtgg cctcgtgcac tgattttaac       60

accatgttat tgtccaatgt gagctttcct cagccggttt cgagtgtaaa actacttgaa      120

gaatatgcta aggatgataa ggttacagtt atatttagtg ttttagatac cttgccaacg      180

gatgaagcca atggaactag atattccaaa tataaggcag acgataatga taaatgtgtt      240

gatcttgcgc ttgtctttca agagctgcag aagaaaggcc ttgtaaagga atttggtaat      300

gaagatactc caccagaaga agtgaatgga ctatggctaa tgagagggaa acatgctgga      360

cacaacatgc ttgaggtttt tgagacgtta gtaaacccga gacgtggttt agaggatctt      420

ccagttaccc caaaatctgc agaggggcga cgtgaagctg tatcccggag taccgcggaa      480

aatcaggctg ggacaaatgg agagagtggg tcctctcaaa ctgcatccaa tgcgggtaca      540

agtcaggtaa ctgctgctag agtatcaggt gtacaagcac aatccgtagc cgcgggcacg      600

aatggaccac agaatgttag ggaggatact gtccccgctc ctgaagcagt tcctagcaat      660

caggatatga agtttctaat ggttctgact aagtgtgatc ttatgatggc aattcctgag      720

gaacagttga gcgggcccaa aaacatgagg gagaaccagg aatcaggatt cactccggct      780

attgcattta cctctcttct accattcctc ttgatgatgt cataa                      825


<210>  12
<211>  1143
<212>  DNA
<213>  Babesia canis


<220>
<221>  gene
<222>  (1)..(1143)

<220>
<221>  Intron
<222>  (170)..(203)

<220>
<221>  Intron
<222>  (408)..(550)

<400>  12
atgatgctgc tcttcgcctt gtctactctt gtcaccttcg ccttctgcga tggtgaaaac       60

actatacttt tatccaatgt agaattccat actccagtat ccagtgtaaa gctgcttaaa      120

gaatacagta gcaatcagga atcaatggcc gttattatga tgctaaccgg taggaaatta      180

tatgcataat tcataattcg cagaaatgcc aaacacatca ggaaagctta ccgatggcaa      240

agttcatgtg gccaatgata acgttaaatg tgctgatttg gctctggctt atcaagaact      300

caaaaaggcc ggcaaggtga catcatggag tccaactgat gacaacgata aggtggtacc      360

tcatggaatc tggttcatag agggcgtcta tgagactgat aagatgtgta agtagatgtg      420

tttatcaacc gtattggggt atactataca attattcagt tgctatatga agtttctggt      480

ttaataattg tgccatgtcc acgccatttg cactattagc attaattaat cgttcctgat      540

gtgttttcag tcgaggttta caagaccctg accgatcctg aggacccaag cgaagttact      600

cgcttgacaa ctgtttctgg tgcttccgga tctgctcaaa gccagcctgc tggtactact      660

gatggtgtct cgggtagcgc tgctagtgcc tctggttcct ctggttccac tacttcccat      720

tctactaccg ctactaccag ctccactagt accgtttcta cttcctcttc tggggcttct      780

acttctagtt ctactgacca ggcatccatg cttaccaccc aaacatccta cagtgcagga      840

tctagcgttc ataaaagcgc tgtggttgct cccactcaga gtaccactcc tgataatgct      900

gaatctggcg caaagcaaag caaagctgcg gttcaggaac ctaagaatgt tttgatgatt      960

ctgaccaagt gtgatcttaa ggccgaagtt accgaggaac agataagaag ccaaggaaac     1020

ccagaaagca atgggtcttc ttctgaaccc actgctgctt ctcctaaact tactaccgct     1080

gcttctggat tcactgccgc cattaccccc ttgttcatgg tcccactcat gtttttcgcc     1140

taa                                                                   1143


<210>  13
<211>  1036
<212>  DNA
<213>  Babesia rossi


<220>
<221>  gene
<222>  (1)..(1036)

<220>
<221>  Intron
<222>  (173)..(207)

<220>
<221>  Intron
<222>  (406)..(569)

<400>  13
atgatgttac tattcgcttt ctcttctctt ctcgcagtgg cctcgtgcac tgattttaac       60

accatgttat tgtccaatgt gagctttcct cagccggttt cgagtgtaaa actacttgaa      120

gaatatgcta aatatcagaa gggttatgtg ttgtatttcc aaatgcgtga tggtacgtcg      180

acattttcag ttatcattat gtgacagatt tgccaaagaa agaggatact gagaggttta      240

gtcaatatgt tcaactggag aacgccgaat gtactgactt ggctgctatc tataatatta      300

tgcaacgtga gggtagaata tttgaataca agcatgaaag cgaacttcct gattatcctg      360

aaggcctgtg gttgttggat gcccaggatg ataaggaaga aattcgtaag tttctgcatt      420

cagcaataca atctgcttaa caatttatac tcatccgtct tgtacattat ttgactgtgt      480

taatctatct tgttgttgat tcttttgtat taattgaaca aatttgtgtc aaaggaatat      540

tataatacac tcaccttata taattgtagt ccacaccttt gagactacac tcccccctac      600

ctctacaaat ggtaatgaac acgctgataa gactagagag gcacctaagc caagaccaga      660

tgctccagct gcttctgata cccaacgtgc ccaagacaat caggaaaaga cacccactga      720

gtcgtcaaca ggttctcgag acactgttca acctcaaact gcccctgcaa cttcaaacgc      780

agtaactggt acgtcatcaa cgacatcaag tgtgcagagc caagctgtat tagggaactc      840

tgaagctact acagggactc agcaatcagc tgaaaatgtt aaggttttaa tggttctgac      900

gaagtgcaac cttaaaatgc atgttactga ggaacagttg agcaagcaca gcaacatacc      960

ccgtaagcat ggatcaggat tcactccggc tattgcattt acctctcttc taccattcct     1020

cttgatgatg tcctaa                                                     1036


<210>  14
<211>  1007
<212>  DNA
<213>  Babesia rossi


<220>
<221>  gene
<222>  (1)..(1007)

<220>
<221>  Intron
<222>  (170)..(204)

<220>
<221>  Intron
<222>  (406)..(552)

<400>  14
atgatgttac tattcgcttt ctcttctctt ctcgcagtgg cctcgtgcac tgattttaac       60

accatgttat tgtccaatgt gagctttcct cagccggttt cgagtgtaaa actacttgaa      120

gaatatgcta aggatgataa ggttacagtt atatttagtg ttttagatag taagttgata      180

taatacgttg atattattgc acagccttgc caacggatga agccaatgga actagatatt      240

ccaaatataa ggcagacgat aatgataaat gtgttgatct tgcgcttgtc tttcaagagc      300

tgcagaagaa aggccttgta aaggaatttg gtaatgaaga tactccacca gaagaagtga      360

atggactatg gctaatgaga gggaaacatg ctggacacaa catgcgtatg tttcttagcg      420

tatactgtct agttttgttt cctatcagtt ataaattatt ctcatttttc ggagtcatct      480

tcttaaccca ttttatttag tacgcgcaag tgattcataa atattattag tgccccttat      540

atatgtttat agttgaggtt tttgagacgt tagtaaaccc gagacgtggt ttagaggatc      600

ttccagttac cccaaaatct gcagaggggc gacgtgaagc tgtatcccgg agtaccgcgg      660

aaaatcaggc tgggacaaat ggagagagtg ggtcctctca aactgcatcc aatgcgggta      720

caagtcaggt aactgctgct agagtatcag gtgtacaagc acaatccgta gccgcgggca      780

cgaatggacc acagaatgtt agggaggata ctgtccccgc tcctgaagca gttcctagca      840

atcaggatat gaagtttcta atggttctga ctaagtgtga tcttatgatg gcaattcctg      900

aggaacagtt gagcgggccc aaaaacatga gggagaacca ggaatcagga ttcactccgg      960

ctattgcatt tacctctctt ctaccattcc tcttgatgat gtcataa                   1007


<210>  15
<211>  8
<212>  PRT
<213>  Babesia rossi

<400>  15

Pro Val Ser Ser Val Lys Leu Leu 
1               5               


