                         SEQUENCE LISTING

<110>  ExxonMobil Research and Engineering Company
 
<120>  Prokaryotic Acyl-ACP Thioesterases For Producing Fatty Acids In Genetically Engineered Microorganisms

<130>  2010EM384 (PM0001)

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  241
<212>  PRT
<213>  Parabacteroides distasonis

<400>  1

Met Glu Lys Val Gly Leu Phe His Phe Val Ala Glu Pro Tyr Leu Met 
1               5                   10                  15      


Asp Phe Arg Gly Arg Val Thr Leu Pro Met Ile Gly Asn Tyr Leu Ile 
            20                  25                  30          


His Ala Ala Ser Ser His Ala Gly Glu Arg Gly Phe Gly Phe Asn Asp 
        35                  40                  45              


Met Ser Glu Arg His Thr Ala Trp Val Leu Ser Arg Leu Ala Ile Glu 
    50                  55                  60                  


Met Lys Glu Tyr Pro Thr Ala Phe Asp Lys Ile Asn Leu Tyr Thr Trp 
65                  70                  75                  80  


Ile Asp Glu Val Gly Arg Leu Phe Thr Ser Arg Cys Phe Glu Leu Ala 
                85                  90                  95      


Asp Glu Asn Gly Lys Thr Phe Gly Phe Ala Arg Ser Ile Trp Ala Ala 
            100                 105                 110         


Ile Asp Val Glu Thr Arg Arg Pro Thr Leu Leu Asp Ile Glu Ala Leu 
        115                 120                 125             


Gly Lys Tyr Ile Asp Glu Arg Pro Cys Pro Ile Glu Lys Pro Gly Lys 
    130                 135                 140                 


Ile Met Pro Ala Glu Asn Lys Ala Glu Gly Ile Pro Tyr Ser Ile Lys 
145                 150                 155                 160 


Tyr Ser Asp Leu Asp Ile Asn Gly His Phe Asn Ser Val Lys Tyr Ile 
                165                 170                 175     


Glu His Leu Leu Asp Leu Phe Asp Ile Asp Gln Phe Lys Thr Arg Glu 
            180                 185                 190         


Ile Gly Arg Leu Glu Ile Ala Tyr Gln Ser Glu Gly Lys Gln Gly Met 
        195                 200                 205             


Pro Leu Thr Leu His Lys Ala Glu Ser Asp Pro Asp Lys Gln Asp Met 
    210                 215                 220                 


Ala Ile Cys His Glu Gly Lys Ala Ile Cys Arg Ala Ala Val Thr Trp 
225                 230                 235                 240 


Arg 
    


<210>  2
<211>  253
<212>  PRT
<213>  Clostridium thermocellum

<400>  2

Met Gln Lys Lys Arg Phe Ser Lys Lys Tyr Glu Val His Tyr Tyr Glu 
1               5                   10                  15      


Ile Asn Ser Met Gln Glu Ala Thr Leu Leu Ser Leu Leu Asn Tyr Met 
            20                  25                  30          


Glu Asp Cys Ala Ile Ser His Ser Thr Ser Ala Gly Tyr Gly Val Asn 
        35                  40                  45              


Glu Leu Leu Ala Ala Asp Ala Gly Trp Val Leu Tyr Arg Trp Leu Ile 
    50                  55                  60                  


Lys Ile Asp Arg Leu Pro Lys Leu Gly Glu Thr Ile Thr Val Gln Thr 
65                  70                  75                  80  


Trp Ala Ser Ser Phe Glu Arg Phe Tyr Gly Asn Arg Glu Phe Ile Val 
                85                  90                  95      


Leu Asp Gly Arg Asp Asn Pro Ile Val Lys Ala Ser Ser Val Trp Ile 
            100                 105                 110         


Tyr Phe Asn Ile Lys Lys Arg Lys Pro Met Arg Ile Pro Leu Glu Met 
        115                 120                 125             


Gly Asp Ala Tyr Gly Ile Asp Glu Thr Arg Ala Leu Glu Glu Pro Phe 
    130                 135                 140                 


Thr Asp Phe Asp Phe Asp Phe Glu Pro Lys Val Ile Glu Glu Phe Thr 
145                 150                 155                 160 


Val Lys Arg Ser Asp Ile Asp Thr Asn Ser His Val Asn Asn Lys Lys 
                165                 170                 175     


Tyr Val Asp Trp Ile Met Glu Thr Val Pro Gln Gln Ile Tyr Asp Asn 
            180                 185                 190         


Tyr Lys Val Thr Ser Leu Gln Ile Ile Tyr Lys Lys Glu Ser Ser Leu 
        195                 200                 205             


Gly Ser Gly Ile Lys Ala Gly Cys Val Ile Asp Glu Gln Asn Thr Asp 
    210                 215                 220                 


Asn Pro Arg Leu Leu His Lys Ile Trp Asp Lys Asn Thr Gly Leu Glu 
225                 230                 235                 240 


Leu Val Ser Ala Glu Thr Ile Trp Gln Lys Ile Gln Ser 
                245                 250             


<210>  3
<211>  726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Parabacteroides distasonis thioesterase gene codon-optimized for expression in Synechocystis 

<400>  3
atggaaaaag tgggtctgtt ccactttgtc gcggagccgt acctcatgga cttccgtggg       60

cgcgtaaccc tacccatgat cggtaactac ctgattcacg ccgcctcctc ccatgccggc      120

gaacggggct ttgggttcaa tgatatgtcc gagcgccata ccgcatgggt gttatcccgc      180

ttggcgattg agatgaagga gtacccgacc gcgttcgaca agatcaacct ctacacctgg      240

attgatgaag tgggccgact cttcacttcc cgttgtttcg agttggccga cgaaaatggc      300

aaaacctttg gcttcgcccg gtccatctgg gctgccattg atgtggaaac gcgacggccg      360

accttgttgg atattgaggc cttgggcaag tatatcgacg agcggccctg tcccatcgag      420

aaaccgggca agattatgcc tgcggaaaac aaagcagaag gcatcccgta ctccattaag      480

tactccgacc tcgacatcaa cgggcacttc aacagcgtca agtacatcga acacctgctc      540

gacctgtttg acatcgacca gttcaagaca cgcgaaatcg gtcggttgga gatcgcctac      600

caatccgaag gcaagcaagg tatgcccttg acgttacata aggccgagag cgacccggat      660

aaacaagaca tggcaatctg ccacgagggt aaagccatct gtcgggcagc cgtgacctgg      720

cggtaa                                                                 726


<210>  4
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Clostridium thermocellum acyl-ACP thioesterase gene codon-optimized for expression in Synechocystis 

<400>  4
atgcaaaaaa agcggttcag caagaagtac gaggtccact actacgagat caacagcatg       60

caggaagcca ccttgctgtc tctcctcaac tacatggagg actgcgccat cagccattcc      120

accagtgccg gctatggcgt gaatgaattg ttggccgcgg acgcggggtg ggtgttgtac      180

cggtggctga tcaagattga ccgcttgccc aagctcggag aaaccatcac cgtgcaaacc      240

tgggcctcct cctttgaacg cttctatggc aaccgagagt tcatcgtgct ggacgggcgc      300

gacaacccca ttgtgaaggc gagcagcgtc tggatctact tcaacatcaa gaagcgcaag      360

cccatgcgga tccccctaga gatgggcgac gcctacggca ttgatgagac ccgagccctg      420

gaggagccgt tcacggattt tgacttcgac ttcgagccca aggtgatcga ggagttcacc      480

gtcaagcgca gtgacattga caccaactcc cacgtgaaca acaagaaata cgtcgactgg      540

atcatggaga cggtacccca acaaatctat gacaattaca aggtcacgtc cctgcagatc      600

atctacaaga aggagagtag cctcggcagc gggattaaag ccgggtgcgt catcgacgaa      660

cagaatacgg acaatccgcg cctgctccac aagatctggg ataaaaacac gggcctcgag      720

ctagtgtccg ccgagaccat ttggcagaaa attcagtcct aa                         762


<210>  5
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for amplifying RS2 from Synechocystis

<400>  5
gggccctatt tgcccgtatt ctgccctatc c                                      31


<210>  6
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for amplifying RS2 from Synechocystis

<400>  6
gggcccgact gcctttggtg gtattaccga tg                                     32


<210>  7
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for amplifying the trcY promoter

<400>  7
actagtcctg aggctgaaat gagctgttga caattaatca tccggctcgt ataatgtgtg       60

gaattgtgag                                                              70


<210>  8
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for amplifying the trcY promoter

<400>  8
ccatggtttt tttcctcctt agtgtgaaat tgttatccgc tcacaattcc acacattata       60

cgagccggat                                                              70


<210>  9
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  trcY promoter sequence

<400>  9
ctgaaatgag ctgttgacaa ttaatcatcc ggctcgtata atgtgtggaa ttgtgagcgg       60

ataacaattt cacactaagg aggaaaaaaa                                        90


<210>  10
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for cloning the codon-optimized Parabacteroides distasonis thioesterase gene

<400>  10
aggaaaaaaa ccatgatgga aaaagtgggt ctgttc                                 36


<210>  11
<211>  42
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for cloning the codon-optimized Parabacteroides distasonis thioesterase gene

<400>  11
cctgcagata tctagattac cgccaggtca cggctgcccg ac                          42


<210>  12
<211>  39
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer for cloning the codon-optimized Clostridium thermocellum acyl-ACP thioesterase gene

<400>  12
aggaaaaaaa ccatgatgca aaaaaagcgg ttcagcaag                              39


<210>  13
<211>  41
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer for cloning the codon-optimized Clostridium thermocellum acyl-ACP thioesterase gene

<400>  13
cctgcagata tctagattag gactgaattt tctgccaaat g                           41


<210>  14
<211>  7646
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pYC expression construct with the codon-optimized Parabacteroides distasonis thioesterase gene

<400>  14
cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc       60

catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg      120

ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat      180

aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat      240

ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg      300

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg      360

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg      420

taaaacgacg gccagtgaat tgggcccgac tgcctttggt ggtattaccg atgagtggca      480

cgttattttc accgctctgg ccgtgttgag catggtgctg ggcaacgtgg tggctttagc      540

ccaaaccagc atgaaacgga tgttggccta ctcttccatc ggtcaagcag gctttgtgat      600

gattggccta gtggccggca gtgaagatgg ttacgccagc atggttttct acatgctcat      660

ctatctgttt atgaacctgg gggcgtttag ttgcattatt ctcttcaccc tccgcactgg      720

cagtgaccaa attagtgatt acgctggtct gtaccacaaa gaccccttgt taaccttggg      780

cttgagcatt tgtttattat ccttgggggg cattcctcct ctggcgggct ttttcggcaa      840

aatttacatc ttctgggccg gttggcaatc gggattgtat ggcctagtcc tacttggtct      900

ggttaccagt gtagtttcca tctactacta catccgggtg gtgaaaatga tggtggtgaa      960

ggagccccag gaaatgtccg aagtaatcaa aaattacccg gccatcaaat ggaatttacc     1020

cggcatgcgt cccctacagg tgggcattgt cgctactttg gttgctacct cgctggcagg     1080

tattctggct aatcccctct ttaacctcgc caccgattcc gtggtcagca ccaagatgtt     1140

gcagacagcc ctccagcaaa caggagaaac tccggcgatc gccatttccc atgatttacc     1200

ctaggggtat caggaaatat tgctttgcag gcaaaagcca atgagtgtaa ctatagaaac     1260

cgatttaaag gagatccact agtcctgagg ctgaaatgag ctgttgacaa ttaatcatcc     1320

ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacactaagg aggaaaaaaa     1380

catgatggaa aaagtgggtc tgttccactt tgtcgcggag ccgtacctca tggacttccg     1440

tgggcgcgta accctaccca tgatcggtaa ctacctgatt cacgccgcct cctcccatgc     1500

cggcgaacgg ggctttgggt tcaatgatat gtccgagcgc cataccgcat gggtgttatc     1560

ccgcttggcg attgagatga aggagtaccc gaccgcgttc gacaagatca acctctacac     1620

ctggattgat gaagtgggcc gactcttcac ttcccgttgt ttcgagttgg ccgacgaaaa     1680

tggcaaaacc tttggcttcg cccggtccat ctgggctgcc attgatgtgg aaacgcgacg     1740

gccgaccttg ttggatattg aggccttggg caagtatatc gacgagcggc cctgtcccat     1800

cgagaaaccg ggcaagatta tgcctgcgga aaacaaagca gaaggcatcc cgtactccat     1860

taagtactcc gacctcgaca tcaacgggca cttcaacagc gtcaagtaca tcgaacacct     1920

gctcgacctg tttgacatcg accagttcaa gacacgcgaa atcggtcggt tggagatcgc     1980

ctaccaatcc gaaggcaagc aaggtatgcc cttgacgtta cataaggccg agagcgaccc     2040

ggataaacaa gacatggcaa tctgccacga gggtaaagcc atctgtcggg cagccgtgac     2100

ctggcggtaa tctagatatc tgcaggccta agctttatgc ttgtaaaccg ttttgtgaaa     2160

aaatttttaa aataaaaaag gggacctcta gggtccccaa ttaattagta atataatcta     2220

ttaaaggtca ttcaaaaggt catccaccgg atcaattccc ctgctcgcgc aggctgggtg     2280

ccaggcccga tccttggagc ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc     2340

cagatccttg acccgcagtt gcaaaccctc actgatccgc atgcccgttc catacagaag     2400

ctgggcgaac aaacgatgct cgccttccag aaaaccgagg atgcgaacca cttcatccgg     2460

ggtcagcacc accggcaagc gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg     2520

cagatccgtg cacagcacct tgccgtagaa gaacagcaag gccgccaatg cctgacgatg     2580

cgtggagacc gaaaccttgc gctcgttcgc cagccaggac agaaatgcct cgacttcgct     2640

gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg     2700

gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg     2760

gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg     2820

ttatgactgt ttttttgggg tacagtctat gcctcggtcg ggcatccaag cagcaagcgc     2880

gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca     2940

gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc     3000

aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac     3060

atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg     3120

ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg     3180

aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg     3240

tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat     3300

ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg     3360

ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg     3420

aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc     3480

tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc     3540

gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg     3600

caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc     3660

ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact     3720

acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc     3780

gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc     3840

acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct     3900

acacaaattg ggagatatat catgaaaggc tggctttttc ttgttatcgc aatagttggc     3960

gaagtaatcg caacatccgc attaaaatct agcgagggct ttactaagct gatccggtgg     4020

atgacctttt gaatgacctt taatagatta tattactaat taattgggga ccctagaggt     4080

cccctttttt attttaaaaa ttttttcaca aaacggttta caagcataaa gcttccgcgg     4140

tacccgggaa ttcgcccttt caagcttcag atcaattcgc gctaacttac attaattgcg     4200

ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc     4260

ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac     4320

cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa     4380

gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg     4440

gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac     4500

gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac     4560

cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga     4620

catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata     4680

tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag     4740

cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc     4800

atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg     4860

aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat     4920

gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac     4980

gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt     5040

aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat     5100

cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc     5160

cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac     5220

cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac     5280

tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg     5340

aaaggttttg caccattcga tggtgtcaac gtaaatgcat gccgcttcgc cttcgcgcaa     5400

gcttagaagg gcgaattccg gacatatgga tcttggggga aattaagacc aaactcgatg     5460

acctccaaaa agatgtaact tctcttaaga tcgatatggc aacggtgaaa accgagttat     5520

ctgcggtcag gatggagata ggtacagtca aggatgatgt taaagatgtc aaagggcggg     5580

ctaatgctca aatttgggcg ttgattcttg ccgtcatcgg agccataatt accaccttgg     5640

tgcgttttgg cattttccct aatccctaac aaaaaagcga ccaggctttt ctttcaattg     5700

cccgatcgcc tttgatattt tcccaaagga taaaagctag tccattcaga atcgagcctt     5760

aaagtactcc catattggct agccccagaa ttactccagc gccgaggatg tggccaaagc     5820

tagcggtgcc cagcacagcc cctaaaccaa agccgccaaa gaagttagag gaaggcatgg     5880

gggtgcccac attttgttgt ttgatggtca atttaccaaa ggcgatcgcc aaaatgttgc     5940

aagcaatcat caccccagca actttagggc tccaggacag ggtggcggga acggcggtgg     6000

ccaacaaaaa gctatgcatt gagattctcc agaataaaga cggtttttaa agggatagcc     6060

ccacgctaat gggggtcttt aaaaatctca tcttacgggg acgctctgcc cctgggaaac     6120

caccgttgca atacttaaca aattttcgtt tttagcttgg caaatgtctt tggcaaaatt     6180

ggttgatctg gcttaaatcg tcagttattt gccctggaat agtctgggga cgggcaattc     6240

tgatcagatt tacccccaac gcttccgcca ctttttgctt aaccaattct cccccctggg     6300

caccggaggc tttagttacc accccttgaa tttgccattg ttgccacagg gctttttcca     6360

atggttcggc tacggggggg cgcaaagcaa tgatacggtc ggaagtaaac ccagcggcga     6420

tcgcctgggc tagggcttgg ggatagggca gaatacgggc aaatagggcc cagcttggcg     6480

taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac     6540

atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca     6600

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat     6660

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc     6720

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca     6780

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca     6840

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg     6900

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg     6960

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt     7020

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt     7080

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc     7140

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt     7200

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt     7260

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc     7320

tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa     7380

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt     7440

tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct     7500

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta     7560

tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa     7620

agtatatatg agtaaacttg gtctga                                          7646


<210>  15
<211>  7677
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pYC expression construct with the codon-optimized Clostridium thermocellum acyl-ACP thioesterase gene

<400>  15
cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc       60

catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg      120

ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat      180

aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat      240

ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg      300

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg      360

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg      420

taaaacgacg gccagtgaat tgggcccgac tgcctttggt ggtattaccg atgagtggca      480

cgttattttc accgctctgg ccgtgttgag catggtgctg ggcaacgtgg tggctttagc      540

ccaaaccagc atgaaacgga tgttggccta ctcttccatc ggtcaagcag gctttgtgat      600

gattggccta gtggccggca gtgaagatgg ttacgccagc atggttttct acatgctcat      660

ctatctgttt atgaacctgg gggcgtttag ttgcattatt ctcttcaccc tccgcactgg      720

cagtgaccaa attagtgatt acgctggtct gtaccacaaa gaccccttgt taaccttggg      780

cttgagcatt tgtttattat ccttgggggg cattcctcct ctggcgggct ttttcggcaa      840

aatttacatc ttctgggccg gttggcaatc gggattgtat ggcctagtcc tacttggtct      900

ggttaccagt gtagtttcca tctactacta catccgggtg gtgaaaatga tggtggtgaa      960

ggagccccag gaaatgtccg aagtaatcaa aaattacccg gccatcaaat ggaatttacc     1020

cggcatgcgt cccctacagg tgggcattgt cgctactttg gttgctacct cgctggcagg     1080

tattctggct aatcccctct ttaacctcgc caccgattcc gtggtcagca ccaagatgtt     1140

gcagacagcc ctccagcaaa caggagaaac tccggcgatc gccatttccc atgatttacc     1200

ctaggggtat caggaaatat tgctttgcag gcaaaagcca atgagtgtaa ctatagaaac     1260

cgatttaaag gagatccact agtcctgagg ctgaaatgag ctgttgacaa ttaatcatcc     1320

ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacactaagg aggaaaaaaa     1380

catgcaaaaa aagcggttca gcaagaagta cgaggtccac tactacgaga tcaacagcat     1440

gcaggaagcc accttgctgt ctctcctcaa ctacatggag gactgcgcca tcagccattc     1500

caccagtgcc ggctatggcg tgaatgaatt gttggccgcg gacgcggggt gggtgttgta     1560

ccggtggctg atcaagattg accgcttgcc caagctcgga gaaaccatca ccgtgcaaac     1620

ctgggcctcc tcctttgaac gcttctatgg caaccgagag ttcatcgtgc tggacgggcg     1680

cgacaacccc attgtgaagg cgagcagcgt ctggatctac ttcaacatca agaagcgcaa     1740

gcccatgcgg atccccctag agatgggcga cgcctacggc attgatgaga cccgagccct     1800

ggaggagccg ttcacggatt ttgacttcga cttcgagccc aaggtgatcg aggagttcac     1860

cgtcaagcgc agtgacattg acaccaactc ccacgtgaac aacaagaaat acgtcgactg     1920

gatcatggag acggtacccc aacaaatcta tgacaattac aaggtcacgt ccctgcagat     1980

catctacaag aaggagagta gcctcggcag cgggattaaa gccgggtgcg tcatcgacga     2040

acagaatacg gacaatccgc gcctgctcca caagatctgg gataaaaaca cgggcctcga     2100

gctagtgtcc gccgagacca tttggcagaa aattcagtcc taatctagat atctgcaggc     2160

ctaagcttta tgcttgtaaa ccgttttgtg aaaaaatttt taaaataaaa aaggggacct     2220

ctagggtccc caattaatta gtaatataat ctattaaagg tcattcaaaa ggtcatccac     2280

cggatcaatt cccctgctcg cgcaggctgg gtgccaggcc cgatccttgg agcccttgcc     2340

ctcccgcacg atgatcgtgc cgtgatcgaa atccagatcc ttgacccgca gttgcaaacc     2400

ctcactgatc cgcatgcccg ttccatacag aagctgggcg aacaaacgat gctcgccttc     2460

cagaaaaccg aggatgcgaa ccacttcatc cggggtcagc accaccggca agcgccgcga     2520

cggccgaggt cttccgatct cctgaagcca gggcagatcc gtgcacagca ccttgccgta     2580

gaagaacagc aaggccgcca atgcctgacg atgcgtggag accgaaacct tgcgctcgtt     2640

cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac     2700

accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct     2760

gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg     2820

tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc     2880

tatgcctcgg tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt     2940

tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca     3000

tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg     3060

agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg     3120

gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa     3180

caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg     3240

agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt     3300

atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta     3360

tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac     3420

atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg     3480

atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg     3540

gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca     3600

aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc     3660

agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct     3720

cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag     3780

tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact     3840

caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc     3900

ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgaaa     3960

ggctggcttt ttcttgttat cgcaatagtt ggcgaagtaa tcgcaacatc cgcattaaaa     4020

tctagcgagg gctttactaa gctgatccgg tggatgacct tttgaatgac ctttaataga     4080

ttatattact aattaattgg ggaccctaga ggtccccttt tttattttaa aaattttttc     4140

acaaaacggt ttacaagcat aaagcttccg cggtacccgg gaattcgccc tttcaagctt     4200

cagatcaatt cgcgctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg     4260

ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg     4320

cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc     4380

cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag     4440

gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc     4500

gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg     4560

cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc     4620

attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc     4680

cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg     4740

cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac     4800

cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg     4860

tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc     4920

aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag     4980

aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac     5040

cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc     5100

gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg     5160

ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg     5220

cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac     5280

accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg     5340

actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc     5400

aacgtaaatg catgccgctt cgccttcgcg caagcttaga agggcgaatt ccggacatat     5460

ggatcttggg ggaaattaag accaaactcg atgacctcca aaaagatgta acttctctta     5520

agatcgatat ggcaacggtg aaaaccgagt tatctgcggt caggatggag ataggtacag     5580

tcaaggatga tgttaaagat gtcaaagggc gggctaatgc tcaaatttgg gcgttgattc     5640

ttgccgtcat cggagccata attaccacct tggtgcgttt tggcattttc cctaatccct     5700

aacaaaaaag cgaccaggct tttctttcaa ttgcccgatc gcctttgata ttttcccaaa     5760

ggataaaagc tagtccattc agaatcgagc cttaaagtac tcccatattg gctagcccca     5820

gaattactcc agcgccgagg atgtggccaa agctagcggt gcccagcaca gcccctaaac     5880

caaagccgcc aaagaagtta gaggaaggca tgggggtgcc cacattttgt tgtttgatgg     5940

tcaatttacc aaaggcgatc gccaaaatgt tgcaagcaat catcacccca gcaactttag     6000

ggctccagga cagggtggcg ggaacggcgg tggccaacaa aaagctatgc attgagattc     6060

tccagaataa agacggtttt taaagggata gccccacgct aatgggggtc tttaaaaatc     6120

tcatcttacg gggacgctct gcccctggga aaccaccgtt gcaatactta acaaattttc     6180

gtttttagct tggcaaatgt ctttggcaaa attggttgat ctggcttaaa tcgtcagtta     6240

tttgccctgg aatagtctgg ggacgggcaa ttctgatcag atttaccccc aacgcttccg     6300

ccactttttg cttaaccaat tctcccccct gggcaccgga ggctttagtt accacccctt     6360

gaatttgcca ttgttgccac agggcttttt ccaatggttc ggctacgggg gggcgcaaag     6420

caatgatacg gtcggaagta aacccagcgg cgatcgcctg ggctagggct tggggatagg     6480

gcagaatacg ggcaaatagg gcccagcttg gcgtaatcat ggtcatagct gtttcctgtg     6540

tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa     6600

gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct     6660

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga     6720

ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc     6780

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     6840

tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt     6900

aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa     6960

aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt     7020

ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg     7080

tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc     7140

agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc     7200

gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta     7260

tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct     7320

acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc     7380

tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa     7440

caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa     7500

aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa     7560

aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt     7620

ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtct        7677


<210>  16
<211>  259
<212>  PRT
<213>  Desulfovibrio desulfuricans subsp. desulfuricans str. G20

<400>  16

Met Arg Cys Ile Gln Arg Asp His Met Ile Pro Phe Ala Gly His Thr 
1               5                   10                  15      


Gly Thr Glu Thr Phe Pro Val Arg Thr Tyr Asp Ala Asp Ser Thr Gly 
            20                  25                  30          


Arg Ala Gly Ile Arg Ala Ile Ala Asp Tyr Phe Gln Glu Ala Ala Ser 
        35                  40                  45              


Gly His Ala Arg Thr Leu Gly Phe Pro Ala Glu Arg Leu Arg Thr Glu 
    50                  55                  60                  


Gln Leu Ala Trp Val Leu Ala Arg Leu Gln Ile Thr Val Asn Arg Phe 
65                  70                  75                  80  


Pro Pro Ala Gly Glu Thr Val Thr Ala Val Thr Trp Pro Ala Ala His 
                85                  90                  95      


Glu Arg His Met Ala Tyr Arg Cys Tyr Glu Leu Tyr Thr Gln Asp Gly 
            100                 105                 110         


Glu Leu Leu Ala Ala Gly Thr Ser Ala Trp Val Thr Ile His Leu Ala 
        115                 120                 125             


Asp Arg Ser Met Val Pro Leu Pro Asp Phe Ile Arg Asp Gly Tyr Pro 
    130                 135                 140                 


Gln Asp Asn Pro Pro Cys Arg Pro Phe Glu Thr Arg Thr Leu Pro Arg 
145                 150                 155                 160 


Leu Arg Glu Glu Ala Ala Gly Val Arg Ile Arg Thr Arg Arg Ala Asp 
                165                 170                 175     


Leu Asp Ile Asn Gly His Val Asn Asn Gly His Tyr Leu Gln Trp Leu 
            180                 185                 190         


Leu Glu Cys Met Pro Cys Asp Arg Gln Asn Glu Leu Arg Ala Val Asp 
        195                 200                 205             


Ile Ser Phe Arg Ala Glu Cys Phe Ala Asp Thr Glu Ile Val Ser Ala 
    210                 215                 220                 


Arg Gly Ala Leu Thr Gly Glu His Thr Val Leu His Cys Ile Arg Thr 
225                 230                 235                 240 


Ala Asp Asp Ala Lys Glu Leu Cys Arg Ala Arg Ser Arg Trp Ala Ala 
                245                 250                 255     


Pro Leu Gly 
            


<210>  17
<211>  243
<212>  PRT
<213>  Elusimicrobium minutum (strain Pei191)

<400>  17

Met Thr Glu Leu Glu Phe Lys Pro Arg Tyr Tyr Glu Ala Ala Leu Asp 
1               5                   10                  15      


Asp Ser Ile Pro Val His Met Leu Cys Asn Tyr Leu Gln Glu Gly Ala 
            20                  25                  30          


Gly Gln Asp Ala Asn Asn Leu Ser Phe Gly Arg Glu Gln Ile Gly Glu 
        35                  40                  45              


His Gly Val Ala Trp Val Leu Ser Arg Met Gln Ile Glu Leu Ile Asn 
    50                  55                  60                  


Lys Ala Val Leu Gly Lys Lys Leu Lys Val Lys Thr Trp Pro Ser Phe 
65                  70                  75                  80  


Ser Glu Lys Ile Ile Ser Arg Arg Glu Tyr Ile Ile Thr Asp Glu Asp 
                85                  90                  95      


Gly Lys Ile Ile Leu Lys Cys Ser Ser Trp Trp Leu Ile Leu Asn Leu 
            100                 105                 110         


Asn Thr Arg Lys Ile Thr Arg Ile Pro Gln His Met Leu Asp Leu Asn 
        115                 120                 125             


Thr Glu Lys Pro Asp Phe Met Val Glu Glu Gly Asn Phe Lys Leu Lys 
    130                 135                 140                 


Thr Pro Gln Asp Ala Lys Pro Val Phe Ser Lys Asp Phe Leu Val Arg 
145                 150                 155                 160 


Leu Glu Asp Ile Asp Cys Asn Gly His Val Asn Asn Thr His Tyr Ile 
                165                 170                 175     


Ala Trp Ala Ile Glu Thr Leu Pro Ala Glu Val Val Lys Asn Lys Thr 
            180                 185                 190         


Ile Lys Ser Leu Arg Ile Asn Phe Lys Ser Glu Cys Ile Asp Gly Asn 
        195                 200                 205             


Lys Ile Lys Ser Phe Val Tyr Asp Asn Gly Asn Ser Glu Tyr Ile His 
    210                 215                 220                 


Glu Leu Val Arg Glu Asp Asp Gly Lys Glu Val Phe Arg Leu Val Ser 
225                 230                 235                 240 


Asn Trp Gln 
            


<210>  18
<211>  220
<212>  PRT
<213>  Carboxydothermus hydrogenoformans Z-2901

<400>  18

Met Asn Ser Asn Ile Phe Glu Leu Glu Tyr Arg Ile Pro Tyr Tyr Asp 
1               5                   10                  15      


Val Asp Tyr Gln Lys Arg Thr Leu Ile Thr Ser Leu Ile Asn Tyr Phe 
            20                  25                  30          


Asn Asp Ile Ala Phe Val Gln Ser Glu Asn Leu Gly Gly Ile Ala Tyr 
        35                  40                  45              


Leu Thr Gln Asn Asn Leu Gly Trp Val Leu Met Asn Trp Asp Ile Lys 
    50                  55                  60                  


Val Asp Arg Tyr Pro Arg Phe Asn Glu Arg Val Leu Val Arg Thr Ala 
65                  70                  75                  80  


Pro His Ser Phe Asn Lys Phe Phe Ala Tyr Arg Trp Phe Glu Ile Tyr 
                85                  90                  95      


Asp Lys Asn Gly Ile Lys Ile Ala Lys Ala Asn Ser Arg Trp Leu Leu 
            100                 105                 110         


Ile Asn Thr Glu Lys Arg Arg Pro Val Lys Ile Asn Asp Tyr Leu Tyr 
        115                 120                 125             


Gly Ile Tyr Gly Val Ser Tyr Glu Asn Asn Asn Ile Leu Pro Ile Glu 
    130                 135                 140                 


Glu Pro Gln Lys Leu Leu Ser Ile Asp Ile Glu Lys Gln Phe Glu Val 
145                 150                 155                 160 


Arg Tyr Ser Asp Leu Asp Ser Asn Gly His Val Asn Asn Val Lys Tyr 
                165                 170                 175     


Val Val Trp Ala Leu Asp Thr Val Pro Leu Glu Ile Ile Ser Asn Tyr 
            180                 185                 190         


Ser Leu Gln Arg Leu Lys Val Lys Tyr Glu Lys Glu Val Thr Tyr Gly 
        195                 200                 205             


Lys Thr Val Arg Val Leu Thr Gly Ile Leu Ser Glu 
    210                 215                 220 


<210>  19
<211>  252
<212>  PRT
<213>  Moorella thermoacetica ATCC 39073

<400>  19

Met Pro Thr Ser Thr Tyr Gln Arg Asp Tyr Glu Val Arg Tyr Tyr Glu 
1               5                   10                  15      


Thr Asn Phe Leu Leu Glu Ala Ser Pro Val Thr Ile Leu Gly Tyr Leu 
            20                  25                  30          


Glu Glu Thr Ala Thr Leu His Ser Glu Thr Ala Gly Ile Gly Ile Asn 
        35                  40                  45              


Lys Leu Lys Ala Ala Gly Arg Gly Trp Val Val Tyr Arg Tyr His Leu 
    50                  55                  60                  


Gln Met Glu Arg Tyr Pro Arg Trp Arg Glu His Ile Thr Ile Thr Thr 
65                  70                  75                  80  


Trp Val Glu Asn Phe Gln Arg Cys Phe Ala His Arg Asp Phe Tyr Ile 
                85                  90                  95      


His Asp Ala Gly Gly Asn Leu Ile Gly Arg Ala Ala Ser Val Trp Val 
            100                 105                 110         


Phe Leu Asp Ile His Lys Lys Lys Pro Leu Arg Ile Pro Pro Gln Val 
        115                 120                 125             


Thr Gly Ala Tyr Gly Leu Tyr Pro Glu Val Ala Val Pro Gly Ala Phe 
    130                 135                 140                 


Thr Asp Leu Pro Ser Leu Glu Lys Pro Ala Thr Ala Gly Glu Phe Thr 
145                 150                 155                 160 


Val Arg Met Ala Asp Leu Asp Thr Asn His His Ala Asn Asn Lys Arg 
                165                 170                 175     


Tyr Ile Gly Trp Ile Leu Glu Gly Val Pro Leu Glu Val His Arg Thr 
            180                 185                 190         


Ala Phe Pro Ala Thr Ile Glu Val Leu Tyr Lys Lys Asp Ala Arg Tyr 
        195                 200                 205             


Gly Glu His Ile His Cys Glu Cys Gln Glu Ile Pro Ala Val Glu Gly 
    210                 215                 220                 


Asp Arg Cys Tyr Leu His Arg Leu Ser Cys Pro Glu Arg Asp Leu Glu 
225                 230                 235                 240 


Leu Thr Leu Ala Arg Thr Thr Trp Arg Lys Arg Arg 
                245                 250         


<210>  20
<211>  247
<212>  PRT
<213>  Geobacter metallireducens GS-15

<400>  20

Met Lys Thr Glu Asn Ser Ile Phe Glu Thr Ser Phe Thr Ile Arg Ser 
1               5                   10                  15      


Phe Asp Val Asp Pro His Gly Phe Val Ser Pro Val Thr Leu Leu Gly 
            20                  25                  30          


Tyr Leu Gln Glu Ala Ala Ser Glu His Met Thr Leu Leu Gly Gly Thr 
        35                  40                  45              


Val Arg Ser Leu Met Ala Glu Gly Leu Thr Trp Val Leu Ser Arg Val 
    50                  55                  60                  


His Leu Ser Ile Glu Arg Tyr Pro Arg Val Arg Asn Glu Met Thr Val 
65                  70                  75                  80  


Arg Thr Trp Pro Ser Leu Arg Glu Gly Arg Phe Thr Cys Arg Glu Phe 
                85                  90                  95      


Glu Leu Leu Asp Arg Thr Gly Ala Val Met Ala Arg Ala Thr Thr Ser 
            100                 105                 110         


Trp Ala Val Ile Asp Phe Lys Thr Arg Arg Ala Val Arg Val Asp Arg 
        115                 120                 125             


His Pro Pro Tyr Pro Leu Thr Pro Arg Arg Ala Ile Asp Asp Asp Phe 
    130                 135                 140                 


Ala Leu Leu Pro Ala Leu Lys Gly Ser Gln Ala Glu Glu Arg Phe Arg 
145                 150                 155                 160 


Val Arg Arg Ser Asp Leu Asp Leu Asn His His Val Asn His Met Val 
                165                 170                 175     


Tyr Ala Gly Trp Ala Leu Asp Ala Val Pro Asp Glu Val Ala Glu Arg 
            180                 185                 190         


His Lys Leu Val Ser Leu Glu Ile Gly Tyr Arg Ala Glu Ala Leu Ala 
        195                 200                 205             


Gly Glu Glu Val Thr Val Cys Cys Ala Cys Ala Glu Asp Asp Asn Gly 
    210                 215                 220                 


Ile Leu Val Ile His Arg Ile Ala Ser Ala Asp Cys Arg Glu Leu Thr 
225                 230                 235                 240 


Arg Leu Arg Thr Arg Trp Arg 
                245         


<210>  21
<211>  248
<212>  PRT
<213>  Salinibacter ruber DSM 13855

<400>  21

Met Thr Ala Gly Ile Trp Thr Asp Glu Val Arg Val Arg Ser Tyr Asp 
1               5                   10                  15      


Val Thr Pro Gln Gly Thr Ala Ser Val Leu Thr Leu Ala Asp Tyr Phe 
            20                  25                  30          


Gln Glu Ala Ala Gly Arg His Ala Ala Glu Leu Gly Val Ser Met Thr 
        35                  40                  45              


Asp Leu Arg Ala Asp Gly Gln Ala Trp Val Leu Ala Phe Met His Met 
    50                  55                  60                  


Gln Val Glu Arg Leu Pro His Gln Asn Glu Ser Leu Arg Ile Glu Thr 
65                  70                  75                  80  


Trp Pro Ser Gly Leu Glu Ser Ala Ser Ala His Arg Glu Phe Val Phe 
                85                  90                  95      


His Asp Glu Glu Gly Thr Val Leu Ala Gly Gly Thr Ser Arg Trp Phe 
            100                 105                 110         


Val Phe Asp Val Asp Arg Arg Arg Pro Val Arg Pro Pro Arg Val Leu 
        115                 120                 125             


Ala Asp Ile Glu Gly Pro Asp Arg Pro Gly Pro Ile Asp Gln Asp Leu 
    130                 135                 140                 


Asp Ala Leu Ser Ala Pro Ala His Thr Asp Arg Glu Gln Thr Phe Thr 
145                 150                 155                 160 


Ala Arg Tyr His Asp Leu Asp Leu Asn Arg His Val Asn Asn Val Arg 
                165                 170                 175     


Tyr Leu Glu Trp Ala Leu Glu Thr Leu Pro Ala Ala Val Leu Asp Glu 
            180                 185                 190         


Arg Arg Cys Leu Gly Phe Ala Val Gln Phe Glu Ala Glu Thr Arg Leu 
        195                 200                 205             


Gly Asp Pro Val Arg Ala Ser Ala Glu Gln Ile Glu Asp Gly Gly Thr 
    210                 215                 220                 


Leu Arg Val Arg His Arg Leu Ala His Ala Glu Ser Asp Gln Thr Leu 
225                 230                 235                 240 


Ala Leu Val Arg Thr Thr Trp Leu 
                245             


<210>  22
<211>  269
<212>  PRT
<213>  Microscilla marina  ATCC 23134

<400>  22

Met Cys Asp Leu Ile Ile Glu Asp Ala Lys Asn Ile Tyr Leu Trp Tyr 
1               5                   10                  15      


Met Glu Lys Asn Gln Thr Thr Gln Leu Pro Lys Ile Trp Leu Asp Phe 
            20                  25                  30          


Glu Val Arg Ala Tyr Glu Val Asp Ile Tyr Asn Arg Val Ser Pro Val 
        35                  40                  45              


Thr Ile Ala Asn Tyr Leu Gln Glu Ala Ala Gly Gln His Ala Asp His 
    50                  55                  60                  


Leu Gly Val Gly Val Thr Asp Leu Leu Lys His Arg Leu Thr Trp Val 
65                  70                  75                  80  


Leu Thr Arg Ile Lys Ile Asp Met Gln Gln Tyr Pro Ser Arg Tyr Glu 
                85                  90                  95      


Pro Val Arg Val Leu Thr Tyr Pro Ile Gly Tyr Asp Lys Tyr Phe Val 
            100                 105                 110         


Tyr Arg Asn Phe Gln Leu Tyr Asn Ala Gln Gly Lys Gln Ile Gly Gln 
        115                 120                 125             


Ala Thr Ser Thr Trp Ala Val Met Asp Ile Gln Ala Arg Lys Met Val 
    130                 135                 140                 


Gly Val Pro Gln Leu Ile Thr Ser Leu Pro Ile Pro Asp Asp Glu Asp 
145                 150                 155                 160 


Phe Ile Thr Arg Thr Lys Gly Lys Ile Ala Lys Val Asn Ala Pro Leu 
                165                 170                 175     


Ser Glu Thr Leu Phe Arg Val Arg Trp Asn Asp Leu Asp Thr Asn Gln 
            180                 185                 190         


His Thr Asn Asn Ala Tyr Tyr Leu Gln Trp Ala Ile Glu Ser Leu Pro 
        195                 200                 205             


Glu Glu Val Leu Lys Ser Arg Gln Leu Ala Ser Ile Asp Leu Leu Tyr 
    210                 215                 220                 


Arg Leu Glu Thr Thr Trp Lys Glu Gly Val Val Ala Arg Thr Glu Gln 
225                 230                 235                 240 


Thr Ser Thr Gln Pro Leu Ser Phe Ile His Gln Leu Ile Arg Glu Ser 
                245                 250                 255     


Asp Gln Lys Glu Leu Ala Gln Ala Thr Thr Val Trp Val 
            260                 265                 


<210>  23
<211>  251
<212>  PRT
<213>  Enterococcus faecalis TX0104

<400>  23

Met Glu Lys Gly Asp Tyr Phe Val Gly Lys Lys His Thr Ser Ser Tyr 
1               5                   10                  15      


Glu Val Ala Tyr Tyr Asp Gly Asp Phe Thr Gly Ala Met Lys Ile Pro 
            20                  25                  30          


Ala Leu Leu Ala Val Val Ile Lys Val Ser Glu Glu Gln Thr Glu Leu 
        35                  40                  45              


Leu Gly Arg Asn Ala Ala Tyr Val Ala Gln Phe Gly Leu Gly Trp Val 
    50                  55                  60                  


Ile Thr Asn Tyr Glu Ile Glu Ile His Arg Leu Pro Lys Val Gly Glu 
65                  70                  75                  80  


Lys Val Ala Ile Thr Thr Gln Ala Met Ser Tyr Asn Lys Tyr Phe Cys 
                85                  90                  95      


Tyr Arg Asn Phe Trp Val His Asp Glu Glu Gly Asn Glu Cys Val Phe 
            100                 105                 110         


Val Lys Ser Thr Phe Val Leu Met Asp Gln Lys Asn Arg Lys Ile Ser 
        115                 120                 125             


Ser Val Leu Pro Glu Ile Ile Ala Pro Phe Asp Ser Glu Lys Ile Thr 
    130                 135                 140                 


Lys Ile Tyr Arg His Glu Lys Ile Glu Lys Val Thr Glu Gly Asn Phe 
145                 150                 155                 160 


Leu Pro Tyr Arg Val Arg Phe Phe Asp Ile Asp Gly Asn Gln His Val 
                165                 170                 175     


Asn Asn Ala Ile Tyr Phe Asn Trp Leu Leu Asp Val Leu Gly Tyr Asp 
            180                 185                 190         


Phe Leu Thr Thr His Gln Pro Lys Lys Ile Leu Val Lys Phe Asp Lys 
        195                 200                 205             


Glu Val Glu Tyr Gly Gln Glu Val Glu Ser His Tyr Glu Ile Val Glu 
    210                 215                 220                 


Gln Glu Asn His Leu Lys Thr Arg His Glu Ile Arg Ile Asp Gly Gln 
225                 230                 235                 240 


Thr Tyr Cys Glu Ala Asn Ile Asp Trp Thr Asn 
                245                 250     


<210>  24
<211>  261
<212>  PRT
<213>  Lactobacillus plantarum JDM1

<400>  24

Met Ala Thr Leu Gly Ala Asn Ala Ser Leu Tyr Ser Glu Gln His Arg 
1               5                   10                  15      


Ile Thr Tyr Tyr Glu Cys Asp Arg Thr Gly Arg Ala Thr Leu Thr Thr 
            20                  25                  30          


Leu Ile Asp Ile Ala Val Leu Ala Ser Glu Asp Gln Ser Asp Ala Leu 
        35                  40                  45              


Gly Leu Thr Thr Glu Met Val Gln Ser His Gly Val Gly Trp Val Val 
    50                  55                  60                  


Thr Gln Tyr Ala Ile Asp Ile Thr Arg Met Pro Arg Gln Asp Glu Val 
65                  70                  75                  80  


Val Thr Val Ala Val Arg Gly Ser Ala Tyr Asn Pro Tyr Phe Ala Tyr 
                85                  90                  95      


Arg Glu Phe Trp Ile Arg Asp Ala Asp Gly Gln Gln Leu Ala Tyr Ile 
            100                 105                 110         


Thr Ser Ile Trp Val Met Met Ser Gln Thr Thr Arg Arg Ile Val Lys 
        115                 120                 125             


Ile Leu Pro Glu Leu Val Ala Pro Tyr Gln Ser Glu Val Val Lys Arg 
    130                 135                 140                 


Ile Pro Arg Leu Pro Arg Pro Ile Ser Phe Glu Ala Thr Asp Thr Thr 
145                 150                 155                 160 


Ile Thr Lys Pro Tyr His Val Arg Phe Phe Asp Ile Asp Pro Asn Arg 
                165                 170                 175     


His Val Asn Asn Ala His Tyr Phe Asp Trp Leu Val Asp Thr Leu Pro 
            180                 185                 190         


Ala Thr Phe Leu Leu Gln His Asp Leu Val His Val Asp Val Arg Tyr 
        195                 200                 205             


Glu Asn Glu Val Lys Tyr Gly Gln Thr Val Thr Ala His Ala Asn Ile 
    210                 215                 220                 


Leu Pro Ser Glu Val Ala Asp Gln Val Thr Thr Ser His Leu Ile Glu 
225                 230                 235                 240 


Val Asp Asp Glu Lys Cys Cys Glu Val Thr Ile Gln Trp Arg Thr Leu 
                245                 250                 255     


Pro Glu Pro Ile Gln 
            260     


<210>  25
<211>  243
<212>  PRT
<213>  Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293

<400>  25

Met Lys Lys Tyr Glu Ile Lys Arg Arg Val Glu Tyr Tyr Glu Ala Asp 
1               5                   10                  15      


Thr Thr Gln Lys Leu Ser Leu Pro Met Ile Leu Asn Tyr Ala Val Leu 
            20                  25                  30          


Ala Ser Lys Cys Gln Ser Asp Glu Leu Gly Val Gly Gln Asp Phe His 
        35                  40                  45              


Leu Gly Arg Gly Leu Gly Trp Ile Ile Leu Gln Tyr Glu Val Ile Ile 
    50                  55                  60                  


Lys Arg Arg Pro Lys Ile Gly Glu Ile Ile Arg Ile Gln Thr Phe Ala 
65                  70                  75                  80  


Thr Gln Tyr Asn Pro Phe Phe Val Arg Arg Pro Phe Val Phe Leu Asp 
                85                  90                  95      


Glu Asn Asp Asn Glu Ile Ile Arg Val Asp Ser Ile Trp Thr Met Ile 
            100                 105                 110         


Asp Met Thr Asn Arg Arg Met Ala Arg Leu Pro Gln Asp Ile Ile Asp 
        115                 120                 125             


Gln Tyr Asp Ala Lys Arg Val Lys Gln Ile Ser Arg Ile Pro Asn Pro 
    130                 135                 140                 


Glu Lys Phe Ser Asp Asp Asp Gln Tyr Thr Glu Arg Asp Tyr His Val 
145                 150                 155                 160 


Arg Tyr Leu Asp Ile Asp Gly Asn Lys His Val Asn Asn Ser Lys Tyr 
                165                 170                 175     


Phe Glu Trp Met Gln Asp Val Ile Ala Pro Glu Tyr Leu Leu Thr His 
            180                 185                 190         


Glu Ile Thr Tyr Ile Asn Leu Lys Phe Glu Asn Glu Ile Arg Leu Gly 
        195                 200                 205             


His Thr Ile Leu Ser Gln Val Val Gln Asn Asp Asn Lys Ser Lys His 
    210                 215                 220                 


Arg Ile Met Met Glu Asn Val Ile Ser Ala Glu Ala Glu Phe Lys Trp 
225                 230                 235                 240 


Arg Lys Ile 
            


<210>  26
<211>  260
<212>  PRT
<213>  Oenococcus oeni ATCC BAA-1163

<400>  26

Met Phe Ile Lys Phe Thr Ile Arg Arg Lys Thr Met Ser Glu Ile Tyr 
1               5                   10                  15      


Ser Glu Glu Leu Tyr Ile Glu Asp Phe Tyr Cys Asp Arg Thr Ala Lys 
            20                  25                  30          


Leu Ser Leu Pro Met Ile Thr Glu Leu Ala Ile Ser Val Ser Ser Lys 
        35                  40                  45              


Gln Thr Val Glu Met Gly Ile Gly Met Gln Arg Leu Val Glu Ala His 
    50                  55                  60                  


His Gly Trp Ile Leu Leu Gln Tyr Asp Ile Lys Ile Asn Arg Arg Pro 
65                  70                  75                  80  


Asn Leu Gly Glu Lys Ile Lys Ile Arg Thr Asp Pro Lys Arg His Asn 
                85                  90                  95      


Arg Phe Phe Ala Phe Arg Asp Phe Asp Phe Phe Asp Gln Asp Gly Asn 
            100                 105                 110         


Thr Leu Ile His Ile Asp Ser Leu Trp Ala Met Ile Asp Leu Lys Arg 
        115                 120                 125             


Arg Arg Leu Val Ser Ile Asp Ser Glu Phe Val Asp Pro Leu Lys Gly 
    130                 135                 140                 


Asn Leu Val Asp Arg Leu Glu Arg Leu Glu Lys Pro Ala Asp Leu Asp 
145                 150                 155                 160 


Arg Ser Phe Ser Gly Ala Ser Ile Ser Val Glu Glu Ile Lys Ala Asn 
                165                 170                 175     


Tyr Phe Asp Ile Asp Thr Asn Gln His Val Asn Asn Ser Asn Tyr Leu 
            180                 185                 190         


Lys Phe Phe Leu Val Pro Val Ala Glu Asn Phe Leu Leu Arg His Glu 
        195                 200                 205             


Pro Lys Arg Ile Leu Ile Lys Tyr Val Lys Glu Ile Arg Leu Asn Gln 
    210                 215                 220                 


Ser Val Val Ser Met Ala Gln Phe Ile Gly Pro Leu His Ser Val His 
225                 230                 235                 240 


Glu Ile Ser Asp Asn Ser Leu Ile Asn Ala Gln Ala Glu Ile Glu Trp 
                245                 250                 255     


Ser Glu Val Glu 
            260 


<210>  27
<211>  282
<212>  PRT
<213>  Mycobacterium smegmatis str. MC2 155

<400>  27

Met Thr Gly Thr Glu Lys Gly Ser Lys Asp Ser Ser Glu Asn Ser Leu 
1               5                   10                  15      


Val Ser Thr Gly Leu Ala Lys Thr Met Met Pro Val Pro Asp Pro His 
            20                  25                  30          


Pro Asp Val Phe Asp Thr Gly Trp Pro Leu Arg Val Ala Asp Ile Asp 
        35                  40                  45              


Arg Asn Gly Arg Leu Arg Phe Asp Ala Ser Thr Arg His Ile Gln Asp 
    50                  55                  60                  


Ile Gly Gln Asp His Leu Arg Gln Leu Gly Phe Glu Asp Thr His Pro 
65                  70                  75                  80  


Ala Trp Ile Val Arg Arg Thr Met Ile Asp Met Ile Glu Pro Ile Glu 
                85                  90                  95      


Phe Pro Glu Leu Leu Arg Leu Arg Arg Trp Cys Ser Gly Thr Ser Asn 
            100                 105                 110         


Arg Trp Cys Glu Met Arg Val Arg Ile Asp Gly Arg Lys Gly Gly Leu 
        115                 120                 125             


Val Glu Ser Glu Ala Phe Trp Ile Asn Ile Asn Arg Glu Thr Gln Gly 
    130                 135                 140                 


Pro Ser Arg Ile Ala Asp Asp Phe Leu Ala Gly Leu Arg Arg Thr Thr 
145                 150                 155                 160 


Asp Ile Asp Arg Leu Arg Trp Lys Pro Tyr Leu Lys Ala Gly Ser Arg 
                165                 170                 175     


Glu Asp Ala Leu Glu Ile Arg Glu Tyr Pro Val Arg Val Ala Asp Ile 
            180                 185                 190         


Asp Leu Phe Asp His Met Asn Asn Ala Val Tyr Trp Thr Val Val Glu 
        195                 200                 205             


Asp Tyr Leu Tyr Thr His Pro Glu Leu Leu Ala Gln Pro Leu Arg Val 
    210                 215                 220                 


Thr Ile Glu His Asp Ala Pro Val Ala Leu Gly Asp Lys Leu Glu Ile 
225                 230                 235                 240 


Ile Ser His Thr His Pro Pro Gly Thr Thr Asp Lys Phe Gly Pro Glu 
                245                 250                 255     


Leu Thr Asp Arg Thr Val Thr Thr Leu Thr Tyr Ala Val Gly Glu Glu 
            260                 265                 270         


Thr Lys Ala Val Ala Cys Leu Phe Ser Leu 
        275                 280         


<210>  28
<211>  271
<212>  PRT
<213>  Mycobacterium vanbaalenii PYR-1

<400>  28

Met Ser Thr Thr Pro Gly Ala Thr Gly Leu Ala Lys Ala Met Met Pro 
1               5                   10                  15      


Val Pro Asp Pro His Pro Asp Val Phe Asp Ile Gln Trp Pro Leu Arg 
            20                  25                  30          


Val Ala Asp Val Asp Arg Glu Gly Arg Leu Lys Phe Asp Ala Ala Thr 
        35                  40                  45              


Arg His Ile Gln Asp Ile Gly Thr Asp Gln Leu Arg Glu Met Gly Tyr 
    50                  55                  60                  


Glu Asp Thr His Pro Leu Trp Ile Val Arg Arg Thr Met Ile Asp Met 
65                  70                  75                  80  


Ile Glu Pro Val Val Phe Lys Asp Met Leu Arg Leu Arg Arg Trp Cys 
                85                  90                  95      


Ser Gly Thr Ser Asn Arg Trp Cys Glu Met Arg Val Arg Ile Glu Gly 
            100                 105                 110         


Arg Lys Gly Gly Leu Ile Glu Ser Glu Ala Phe Trp Ile Asn Ile Asn 
        115                 120                 125             


Arg Glu Thr Gln Gly Pro Ala Arg Ile Ser Asp Asp Phe Ile Glu Gly 
    130                 135                 140                 


Leu Arg Arg Thr Thr Asp Glu Asn Arg Leu Arg Trp Lys Pro Tyr Leu 
145                 150                 155                 160 


Arg Ala Gly Ser Arg Glu Asp Ala Glu His Ile Arg Asp Tyr Pro Val 
                165                 170                 175     


Arg Val Ser Asp Ile Asp Ile Phe Asp His Met Asn Asn Ser Val Tyr 
            180                 185                 190         


Trp Ser Val Val Glu Asp Tyr Leu Tyr Ser Gln Pro Glu Leu Met Ser 
        195                 200                 205             


Ala Pro Val Arg Val Thr Ile Glu His Asp Leu Pro Val Ala Leu Gly 
    210                 215                 220                 


Asp Lys Leu Glu Ile Ile Arg His Val His Pro Ala Gly Ser Thr Asp 
225                 230                 235                 240 


Lys Phe Gly Glu Glu Leu Ser Asp Arg Thr Val Thr Thr Leu Thr Tyr 
                245                 250                 255     


Ala Val Gly Asn Glu Thr Lys Ala Val Ala Ala Ile Phe Pro Leu 
            260                 265                 270     


<210>  29
<211>  256
<212>  PRT
<213>  Rhodococcus erythropolis SK121

<400>  29

Met Asn Arg Thr Lys Val Asp Asp His Val Ala Met Ala Glu Leu Pro 
1               5                   10                  15      


Thr Thr Gly Ser Ile Phe Gln Ala Ser Trp Pro Val Arg Thr Gly Asp 
            20                  25                  30          


Ile His Thr Asp Lys Gln Leu Arg Leu Asp Ala Ile Ala Arg Tyr Leu 
        35                  40                  45              


Gln Asp Ala Gly Phe Asp Asn Leu Val Asp Arg Gly Ala Met Asp Thr 
    50                  55                  60                  


His Pro Leu Trp Met Val Arg Arg Thr Val Ile Asp Val Leu Glu Pro 
65                  70                  75                  80  


Val Val Trp Pro Asp Arg Val His Leu Gln Arg Trp Cys Ser Gly Leu 
                85                  90                  95      


Ser Asn Lys Trp Cys Ser Met Arg Val Arg Ile Arg Ser Asp Gly Gly 
            100                 105                 110         


Gly Leu Ile Glu Thr Glu Ala Phe Trp Ile Asn Ile Asp Pro Lys Thr 
        115                 120                 125             


Gly Met Pro Ala Ser Ile Ser Glu Lys Phe Thr Ala Ala Leu Ala Ser 
    130                 135                 140                 


Thr Ala Val Asp Gln His Leu His Trp Arg Arg Trp Ile Asp Pro Thr 
145                 150                 155                 160 


Pro Asp Thr Thr Pro Ser Ala Asp Thr Pro Phe Pro Leu Arg Ser Ser 
                165                 170                 175     


Asp Phe Asp Pro Phe Asp His Val Asn Asn Ala Ile Tyr Trp Gln Pro 
            180                 185                 190         


Val Glu Asp Ala Leu Pro Asp Arg Leu Arg Ser Gly Pro Phe Arg Ala 
        195                 200                 205             


Ile Leu Glu Tyr Thr Gln Pro Ile Lys Arg Gly Glu Gln Val Ser Val 
    210                 215                 220                 


Arg Thr Gly Thr Asn Thr Leu His Ile Asp Ala Gly Asp Glu Ala Arg 
225                 230                 235                 240 


Ala Ser Ala Arg Trp Phe Ala Leu Ser Pro Lys Ser Asn Asp Gln Gly 
                245                 250                 255     


<210>  30
<211>  259
<212>  PRT
<213>  Rhodococcus opacus B4

<400>  30

Met Ala Leu Asp Arg Pro Leu Ala Ala Pro Pro Ser Arg Gly Arg Phe 
1               5                   10                  15      


Phe Glu Thr Ser Trp Pro Val Arg Thr Gly Asp Ile Asp Ala Ala Lys 
            20                  25                  30          


Arg Leu Arg Leu Asp Gly Ile Ala Arg Tyr Leu Gln Asp Ala Gly Leu 
        35                  40                  45              


Asp Asn Leu Asp Ala Val Asp Ala Ala Asp Ser His Pro Leu Trp Ile 
    50                  55                  60                  


Val Arg Arg Thr Val Ile Asp Val Leu Arg Pro Ala Val Trp Pro Glu 
65                  70                  75                  80  


Arg Val His Leu Arg Arg Trp Cys Ser Ala Leu Ser Thr Arg Trp Thr 
                85                  90                  95      


Asn Met Arg Val Gln Ile Arg Gly Glu Ala Gly Ala Leu Ile Glu Thr 
            100                 105                 110         


Glu Gly Phe Trp Ile His Ile Ser Gly Glu Thr Gly Met Pro Thr Arg 
        115                 120                 125             


Ile Asp Asp Gly Phe Ile Glu Arg Leu Gly Glu Ser Ala Glu Glu His 
    130                 135                 140                 


Arg Leu Lys Trp Lys Arg Trp Leu Val Glu Asn Ala Pro Ala Ala Asp 
145                 150                 155                 160 


Glu Asp Gly Ile Glu Asp Ser Glu Phe Val Leu Arg Arg Thr Asp Ile 
                165                 170                 175     


Asp Pro Phe Asp His Val Asn Asn Ala Val Tyr Trp Gln Ala Val Glu 
            180                 185                 190         


Glu Leu Leu Ala Asp His Glu His Pro Gly Gly Gly Arg Leu Val Asp 
        195                 200                 205             


Asn Pro His Arg Ala Val Leu Glu Tyr Leu Ala Pro Ile Val Ser Thr 
    210                 215                 220                 


Asp Lys Ile Val Leu Arg Ser Arg Arg Asp Asp Thr Ser Leu Thr Val 
225                 230                 235                 240 


Trp Phe Leu Val Asp Asp Ala Leu Arg Ala Val Ala His Val Gly Pro 
                245                 250                 255     


Leu Thr Ala 
            


