                         SEQUENCE LISTING

<110>  Philip Morris Products S.A
 
<120>  Method for Producing Mullerian Inhibitor Substance in Plants

<130>  307486-20642

<140>  EP 10016035.7
<141>  2010-12-23

<160>  18    

<170>  PatentIn version 3.3

<210>  1
<211>  330
<212>  DNA
<213>  Artificial sequence

<220>
<221>  Source
<222>  1..330
<223> /note= "Description of artificial sequence:  Nucleotide sequence of human
              mature MIS optimised for expression in plants"

<400>  1
tcagctggtg ctaccgctgc tgatgggcct tgcgccttga gggagttatc tgtggacctg     60

cgagcagaaa ggtcagttct aatacctgag acttatcaag caaacaattg tcaaggtgtc    120

tgcggttggc cccaaagtga taggaatcca cgttatggta accatgtagt tcttctcttg    180

aaaatgcaag ctcgtggtgc tgccctagct cgaccaccct gttgtgttcc tacagcctac    240

gccggaaagt tacttatcag tttatcagaa gaaagaatca gtgctcacca cgttcctaat    300

atggtcgcta cagagtgtgg atgcagataa                                     330


<210>  2
<211>  109
<212>  PRT
<213>  Artificial sequence

<220>
<221>  Source
<222>  1..109
<223> /note= "Description of artificial sequence: Protein sequence of human 
              mature MIS optimised for expression in plants"

<400>  2

Ser Ala Gly Ala Thr Ala Ala Asp Gly Pro Cys Ala Leu Arg Glu Leu 
1               5                   10                  15      


Ser Val Asp Leu Arg Ala Glu Arg Ser Val Leu Ile Pro Glu Thr Tyr 
            20                  25                  30          


Gln Ala Asn Asn Cys Gln Gly Val Cys Gly Trp Pro Gln Ser Asp Arg 
        35                  40                  45              


Asn Pro Arg Tyr Gly Asn His Val Val Leu Leu Leu Lys Met Gln Ala 
    50                  55                  60                  


Arg Gly Ala Ala Leu Ala Arg Pro Pro Cys Cys Val Pro Thr Ala Tyr 
65                  70                  75                  80  


Ala Gly Lys Leu Leu Ile Ser Leu Ser Glu Glu Arg Ile Ser Ala His 
                85                  90                  95      


His Val Pro Asn Met Val Ala Thr Glu Cys Gly Cys Arg 
            100                 105                 


<210>  3
<211>  1889
<212>  DNA
<213>  Zea mays

<400>  3
gcaccagttt caacgatcgt cccgcgtcaa tattattaaa aaactcttac atttctttat     60

aatcaacccg cactcttata atctcttctc tactactata ataagagagt ttatgtacaa    120

aataaggtga aattatgtat aagtgttctg gatattggtt gttggctcca tattcacaca    180

acctaatcaa tagaaaacat atgttttatt aaaacaaaat ttatcatata tcatatatat    240

atatatacat atatatatat atatataaac cgtagcaatg cacgggcata taactagtgc    300

aacttaatac atgtgtgtat taagatgaat aagagggtat ccaaataaaa aacttgttcg    360

cttacgtctg gatcgaaagg ggttggaaac gattaaatct cttcctagtc aaaattgaat    420

agaaggagat ttaatctctc ccaatcccct tcgatcatcc aggtgcaacc gtataagtcc    480

taaagtggtg aggaacacga aacaaccatg cattggcatg taaagctcca agaatttgtt    540

gtatccttaa caactcacag aacatcaacc aaaattgcac gtcaagggta ttgggtaaga    600

aacaatcaaa caaatcctct ctgtgtgcaa agaaacacgg tgagtcatgc cgagatcata    660

ctcatctgat atacatgctt acagctcaca agacattaca aacaactcat attgcattac    720

aaagatcgtt tcatgaaaaa taaaataggc cggacaggac aaaaatcctt gacgtgtaaa    780

gtaaatttac aacaaaaaaa aagccatatg tcaagctaaa tctaattcgt tttacgtaga    840

tcaacaacct gtagaaggca acaaaactga gccacgcaga agtacagaat gattccagat    900

gaaccatcga cgtgctacgt aaagagagtg acgagtcata tacatttggc aagaaaccat    960

gaagctgcct acagccgtct cggtggcata agaacacaag aaattgtgtt aattaatcaa   1020

agctataaat aacgctcgca tgcctgtgca cttctccatc accaccactg ggtcttcaga   1080

ccattagctt tatctactcc agagcgcaga agaacccgat cgacaccatg agggtgttgc   1140

tcgttgccct cgctctcctg gctctcgctg cgagcgccac ctccacgcat acaagcggcg   1200

gctgcggctg ccagccaccg ccgccggttc atctaccgcc gccggtgcat ctgccacctc   1260

cggttcacct gccacctccg gtgcatctcc caccgccggt ccacctgccg ccgccggtcc   1320

acctgccacc gccggtccat gtgccgccgc cggttcatct gccgccgcca ccatgccact   1380

accctactca accgccccgg cctcagcctc atccccagcc acacccatgc ccgtgccaac   1440

agccgcatcc aagcccgtgc cagctgcagg gaacctgcgg cgttggcagc accccgatcc   1500

tgggccagtg cgtcgagttc ctgaggcatc agtgcagccc gacggcgacg ccctactgct   1560

cgcctcagtg ccagtcgttg cggcagcagt gttgccagca gctcaggcag gtggagccgc   1620

agcaccggta ccaggcgatc ttcggcttgg tcctccagtc catcctgcag cagcagccgc   1680

aaagcggcca ggtcgcgggg ctgttggcgg cgcagatagc gcagcaactg acggcgatgt   1740

gcggcctgca gcagccgact ccatgcccct acgctgctgc cggcggtgtc ccccactgaa   1800

gaaactatgt gctgtagtat agccgctggc tagctagcta gttgagtcat ttagcggcga   1860

tgattgagta ataatgtgtc acgcatcac                                     1889


<210>  4
<211>  223
<212>  PRT
<213>  Zea mays

<400>  4

Met Arg Val Leu Leu Val Ala Leu Ala Leu Leu Ala Leu Ala Ala Ser 
1               5                   10                  15      


Ala Thr Ser Thr His Thr Ser Gly Gly Cys Gly Cys Gln Pro Pro Pro 
            20                  25                  30          


Pro Val His Leu Pro Pro Pro Val His Leu Pro Pro Pro Val His Leu 
        35                  40                  45              


Pro Pro Pro Val His Leu Pro Pro Pro Val His Leu Pro Pro Pro Val 
    50                  55                  60                  


His Leu Pro Pro Pro Val His Val Pro Pro Pro Val His Leu Pro Pro 
65                  70                  75                  80  


Pro Pro Cys His Tyr Pro Thr Gln Pro Pro Arg Pro Gln Pro His Pro 
                85                  90                  95      


Gln Pro His Pro Cys Pro Cys Gln Gln Pro His Pro Ser Pro Cys Gln 
            100                 105                 110         


Leu Gln Gly Thr Cys Gly Val Gly Ser Thr Pro Ile Leu Gly Gln Cys 
        115                 120                 125             


Val Glu Phe Leu Arg His Gln Cys Ser Pro Thr Ala Thr Pro Tyr Cys 
    130                 135                 140                 


Ser Pro Gln Cys Gln Ser Leu Arg Gln Gln Cys Cys Gln Gln Leu Arg 
145                 150                 155                 160 


Gln Val Glu Pro Gln His Arg Tyr Gln Ala Ile Phe Gly Leu Val Leu 
                165                 170                 175     


Gln Ser Ile Leu Gln Gln Gln Pro Gln Ser Gly Gln Val Ala Gly Leu 
            180                 185                 190         


Leu Ala Ala Gln Ile Ala Gln Gln Leu Thr Ala Met Cys Gly Leu Gln 
        195                 200                 205             


Gln Pro Thr Pro Cys Pro Tyr Ala Ala Ala Gly Gly Val Pro His 
    210                 215                 220             


<210>  5
<211>  112
<212>  PRT
<213>  Zea mays

<400>  5

Met Arg Val Leu Leu Val Ala Leu Ala Leu Leu Ala Leu Ala Ala Ser 
1               5                   10                  15      


Ala Thr Ser Thr His Thr Ser Gly Gly Cys Gly Cys Gln Pro Pro Pro 
            20                  25                  30          


Pro Val His Leu Pro Pro Pro Val His Leu Pro Pro Pro Val His Leu 
        35                  40                  45              


Pro Pro Pro Val His Leu Pro Pro Pro Val His Leu Pro Pro Pro Val 
    50                  55                  60                  


His Leu Pro Pro Pro Val His Val Pro Pro Pro Val His Leu Pro Pro 
65                  70                  75                  80  


Pro Pro Cys His Tyr Pro Thr Gln Pro Pro Arg Pro Gln Pro His Pro 
                85                  90                  95      


Gln Pro His Pro Cys Pro Cys Gln Gln Pro His Pro Ser Pro Cys Gln 
            100                 105                 110         


<210>  6
<211>  43
<212>  PRT
<213>  Artificial sequence

<220>
<221>  Source
<222>  1..43
<223> /note= "Description of artificial sequence: Amino acid sequence of
             (PPPAPA)n"

<400>  6

Ala Pro Ala Pro Pro Pro Ala Pro Ala Pro Pro Pro Ala Pro Ala Pro 
1               5                   10                  15      


Pro Pro Ala Pro Ala Pro Pro Pro Ala Pro Ala Pro Pro Pro Ala Pro 
            20                  25                  30          


Ala Pro Pro Pro Ala Pro Ala Pro Pro Pro Ala 
        35                  40              


<210>  7
<211>  43
<212>  PRT
<213>  Artificial sequence

<220>
<221>  Source
<222>  1..43
<223> /note= "Description of artificial sequence: Amino acid sequence of
             (PPPEPE)n"

<400>  7

Glu Pro Ala Pro Pro Pro Glu Pro Glu Pro Pro Pro Glu Pro Glu Pro 
1               5                   10                  15      


Pro Pro Glu Pro Glu Pro Pro Pro Glu Pro Glu Pro Pro Pro Glu Pro 
            20                  25                  30          


Glu Pro Pro Pro Glu Pro Glu Pro Pro Pro Glu 
        35                  40              


<210>  8
<211>  44
<212>  PRT
<213>  Artificial sequence

<220>
<221>  Source
<222>  1..44
<223> /note= "Description of artificial sequence: Amino acid sequence of
             (PPPVEL)n"

<400>  8

Val Glu Leu Pro Pro Pro Val Glu Leu Pro Pro Pro Val Glu Leu Pro 
1               5                   10                  15      


Pro Pro Val Glu Leu Pro Pro Pro Val Glu Leu Pro Pro Pro Val Glu 
            20                  25                  30          


Leu Pro Pro Pro Val Glu Val Pro Pro Pro Val Glu 
        35                  40                  


<210>  9
<211>  44
<212>  PRT
<213>  Artificial sequence

<220>
<221>  Source
<222>  1..44
<223> /note= "Description of artificial sequence: Amino acid sequence of
             (PPPVAL)n"

<400>  9

Val Ala Leu Pro Pro Pro Val Ala Leu Pro Pro Pro Val Ala Leu Pro 
1               5                   10                  15      


Pro Pro Val Ala Leu Pro Pro Pro Val Ala Leu Pro Pro Pro Val Ala 
            20                  25                  30          


Leu Pro Pro Pro Val Ala Val Pro Pro Pro Val Ala 
        35                  40                  


<210>  10
<211>  44
<212>  PRT
<213>  Artificial sequence

<220>
<221>  Source
<222>  1..44
<223> /note= "Description of artificial sequence: Amino acid sequence of
              (PPPVTL)n"

<400>  10

Val Thr Leu Pro Pro Pro Val Thr Leu Pro Pro Pro Val Thr Leu Pro 
1               5                   10                  15      


Pro Pro Val Thr Leu Pro Pro Pro Val Thr Leu Pro Pro Pro Val Thr 
            20                  25                  30          


Leu Pro Pro Pro Val Thr Val Pro Pro Pro Val Thr 
        35                  40                  


<210>  11
<211>  29
<212>  PRT
<213>  Artificial sequence

<220>
<221>  Source
<222>  1..29
<223> /note= "Description of artificial sequence: Amino acid sequence of
             (PPPAPA)n"

<400>  11

Pro Pro Pro Ala Pro Ala Pro Pro Pro Ala Pro Ala Pro Pro Pro Ala 
1               5                   10                  15      


Pro Cys Pro Cys Pro Ala Pro Ala Pro Pro Pro Cys Pro 
            20                  25                  


<210>  12
<211>  29
<212>  PRT
<213>  Artificial sequence

<220>
<221>  Source
<222>  1..29
<223> /note= "Description of artificial sequence: Amino acid sequence of
              (PPPEPE)n"

<400>  12

Pro Pro Pro Glu Pro Glu Pro Pro Pro Glu Pro Glu Pro Pro Pro Glu 
1               5                   10                  15      


Pro Cys Pro Cys Pro Glu Pro Glu Pro Pro Pro Cys Pro 
            20                  25                  


<210>  13
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<221>  Source
<222>  1..6
<223>  /note="Description of artificial sequence: proline-rich 
       non-naturally occurring repeat sequence."

<400>  13

Pro Pro Pro Val Thr Leu 
1               5       

<210>  14
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<221>  Source
<222>  1..7
<223> /note= "Description of artificial sequence: Optimum cleavage
              recognition site for Tobacco Etch Virus protease"

<400>  14

Glu Asn Leu Tyr Phe Gln Gly 
1               5           

<210>  15
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<221>  Source
<222>  1..7
<223> /note= "Description of artificial sequence: Optimum cleavage
              recognition site for Tobacco Etch Virus protease"

<400>  15

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  16
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<221>  Source
<222>  1..4
<223> /note= "Description of artificial sequence: Linker sequence"

<400>  16

Gly Gly Gly Gly 
1               

<210>  17
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<221>  Source
<222>  1..15
<223> /note= "Description of artificial sequence: Linker sequence"

<400>  17

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  18
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<221>  Source
<222>  1..5
<223> /note= "Description of artificial sequence: Linker sequence"

<400>  18

Gly Gly Gly Gly Gly 
1               5   

