                         SEQUENCE LISTING

<110>  Philip Morris Products S.A.
 
<120>  Method for Expressing Deoxyribonuclease in Plants

<130>  307486-20640

<140>  EP 10016033.2
<141>  2010-12-23

<160>  18    

<170>  PatentIn version 3.3

<210>  1
<211>  783
<212>  DNA
<213>  Artificial sequence

<220>
<221>  source
<222>  1..783
<223>  /note= "Description of artificial sequence: DNA sequence of plant
optimised human DNaseI"

<400>  1
ctcaagattg ctgctttcaa catccaaact ttcggagaga ctaagatgtc taacgctact     60

cttgtgtcct acatcgttca gattctctcc agatacgata ttgctcttgt tcaggaagtt    120

agggattctc accttactgc tgtgggaaag cttcttgata acctcaatca ggatgctcca    180

gatacttacc actacgttgt gtctgaacca cttggaagaa actcctacaa agagcgttac    240

ctctttgttt accgtccaga tcaagtttct gctgtggatt cctactacta cgatgatgga    300

tgtgagccat gcggaaacga tactttcaat agagagccag ctatcgttcg ttttttcagt    360

aggttcactg aagttcgtga gtttgctatt gtgccacttc atgctgctcc aggtgatgct    420

gttgctgaga ttgatgctct ctacgatgtg taccttgatg ttcaagagaa gtggggactt    480

gaggatgtta tgctcatggg agatttcaat gctggatgct cttatgttag gccatctcag    540

tggtcatcta ttaggctttg gacttcccca actttccaat ggcttatccc agattccgct    600

gatacaactg ctactccaac tcattgtgct tacgatagga ttgtggtggc tggaatgctt    660

cttagaggtg ctgttgttcc agattctgct ctcccattca atttccaagc tgcttacgga    720

ctttctgatc aacttgctca ggctatttct gatcactacc cagttgaggt gatgcttaag    780

tga                                                                  783


<210>  2
<211>  260
<212>  PRT
<213>  Artificial sequence

<220>
<221>  source
<222>  1..260
<223>  /note= "Description of artificial sequence: Amino acid sequence of
plant optimised DNaseI"

<400>  2

Leu Lys Ile Ala Ala Phe Asn Ile Gln Thr Phe Gly Glu Thr Lys Met 
1               5                   10                  15      


Ser Asn Ala Thr Leu Val Ser Tyr Ile Val Gln Ile Leu Ser Arg Tyr 
            20                  25                  30          


Asp Ile Ala Leu Val Gln Glu Val Arg Asp Ser His Leu Thr Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Asn Leu Asn Gln Asp Ala Pro Asp Thr Tyr His 
    50                  55                  60                  


Tyr Val Val Ser Glu Pro Leu Gly Arg Asn Ser Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Val Tyr Arg Pro Asp Gln Val Ser Ala Val Asp Ser Tyr Tyr 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Pro Cys Gly Asn Asp Thr Phe Asn Arg Glu 
            100                 105                 110         


Pro Ala Ile Val Arg Phe Phe Ser Arg Phe Thr Glu Val Arg Glu Phe 
        115                 120                 125             


Ala Ile Val Pro Leu His Ala Ala Pro Gly Asp Ala Val Ala Glu Ile 
    130                 135                 140                 


Asp Ala Leu Tyr Asp Val Tyr Leu Asp Val Gln Glu Lys Trp Gly Leu 
145                 150                 155                 160 


Glu Asp Val Met Leu Met Gly Asp Phe Asn Ala Gly Cys Ser Tyr Val 
                165                 170                 175     


Arg Pro Ser Gln Trp Ser Ser Ile Arg Leu Trp Thr Ser Pro Thr Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Thr Pro Thr His 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Met Leu Leu Arg Gly Ala 
    210                 215                 220                 


Val Val Pro Asp Ser Ala Leu Pro Phe Asn Phe Gln Ala Ala Tyr Gly 
225                 230                 235                 240 


Leu Ser Asp Gln Leu Ala Gln Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Met Leu Lys 
            260 


<210>  3
<211>  1889
<212>  DNA
<213>  Zea mays

<400>  3
gcaccagttt caacgatcgt cccgcgtcaa tattattaaa aaactcttac atttctttat     60

aatcaacccg cactcttata atctcttctc tactactata ataagagagt ttatgtacaa    120

aataaggtga aattatgtat aagtgttctg gatattggtt gttggctcca tattcacaca    180

acctaatcaa tagaaaacat atgttttatt aaaacaaaat ttatcatata tcatatatat    240

atatatacat atatatatat atatataaac cgtagcaatg cacgggcata taactagtgc    300

aacttaatac atgtgtgtat taagatgaat aagagggtat ccaaataaaa aacttgttcg    360

cttacgtctg gatcgaaagg ggttggaaac gattaaatct cttcctagtc aaaattgaat    420

agaaggagat ttaatctctc ccaatcccct tcgatcatcc aggtgcaacc gtataagtcc    480

taaagtggtg aggaacacga aacaaccatg cattggcatg taaagctcca agaatttgtt    540

gtatccttaa caactcacag aacatcaacc aaaattgcac gtcaagggta ttgggtaaga    600

aacaatcaaa caaatcctct ctgtgtgcaa agaaacacgg tgagtcatgc cgagatcata    660

ctcatctgat atacatgctt acagctcaca agacattaca aacaactcat attgcattac    720

aaagatcgtt tcatgaaaaa taaaataggc cggacaggac aaaaatcctt gacgtgtaaa    780

gtaaatttac aacaaaaaaa aagccatatg tcaagctaaa tctaattcgt tttacgtaga    840

tcaacaacct gtagaaggca acaaaactga gccacgcaga agtacagaat gattccagat    900

gaaccatcga cgtgctacgt aaagagagtg acgagtcata tacatttggc aagaaaccat    960

gaagctgcct acagccgtct cggtggcata agaacacaag aaattgtgtt aattaatcaa   1020

agctataaat aacgctcgca tgcctgtgca cttctccatc accaccactg ggtcttcaga   1080

ccattagctt tatctactcc agagcgcaga agaacccgat cgacaccatg agggtgttgc   1140

tcgttgccct cgctctcctg gctctcgctg cgagcgccac ctccacgcat acaagcggcg   1200

gctgcggctg ccagccaccg ccgccggttc atctaccgcc gccggtgcat ctgccacctc   1260

cggttcacct gccacctccg gtgcatctcc caccgccggt ccacctgccg ccgccggtcc   1320

acctgccacc gccggtccat gtgccgccgc cggttcatct gccgccgcca ccatgccact   1380

accctactca accgccccgg cctcagcctc atccccagcc acacccatgc ccgtgccaac   1440

agccgcatcc aagcccgtgc cagctgcagg gaacctgcgg cgttggcagc accccgatcc   1500

tgggccagtg cgtcgagttc ctgaggcatc agtgcagccc gacggcgacg ccctactgct   1560

cgcctcagtg ccagtcgttg cggcagcagt gttgccagca gctcaggcag gtggagccgc   1620

agcaccggta ccaggcgatc ttcggcttgg tcctccagtc catcctgcag cagcagccgc   1680

aaagcggcca ggtcgcgggg ctgttggcgg cgcagatagc gcagcaactg acggcgatgt   1740

gcggcctgca gcagccgact ccatgcccct acgctgctgc cggcggtgtc ccccactgaa   1800

gaaactatgt gctgtagtat agccgctggc tagctagcta gttgagtcat ttagcggcga   1860

tgattgagta ataatgtgtc acgcatcac                                     1889


<210>  4
<211>  223
<212>  PRT
<213>  Zea mays

<400>  4

Met Arg Val Leu Leu Val Ala Leu Ala Leu Leu Ala Leu Ala Ala Ser 
1               5                   10                  15      


Ala Thr Ser Thr His Thr Ser Gly Gly Cys Gly Cys Gln Pro Pro Pro 
            20                  25                  30          


Pro Val His Leu Pro Pro Pro Val His Leu Pro Pro Pro Val His Leu 
        35                  40                  45              


Pro Pro Pro Val His Leu Pro Pro Pro Val His Leu Pro Pro Pro Val 
    50                  55                  60                  


His Leu Pro Pro Pro Val His Val Pro Pro Pro Val His Leu Pro Pro 
65                  70                  75                  80  


Pro Pro Cys His Tyr Pro Thr Gln Pro Pro Arg Pro Gln Pro His Pro 
                85                  90                  95      


Gln Pro His Pro Cys Pro Cys Gln Gln Pro His Pro Ser Pro Cys Gln 
            100                 105                 110         


Leu Gln Gly Thr Cys Gly Val Gly Ser Thr Pro Ile Leu Gly Gln Cys 
        115                 120                 125             


Val Glu Phe Leu Arg His Gln Cys Ser Pro Thr Ala Thr Pro Tyr Cys 
    130                 135                 140                 


Ser Pro Gln Cys Gln Ser Leu Arg Gln Gln Cys Cys Gln Gln Leu Arg 
145                 150                 155                 160 


Gln Val Glu Pro Gln His Arg Tyr Gln Ala Ile Phe Gly Leu Val Leu 
                165                 170                 175     


Gln Ser Ile Leu Gln Gln Gln Pro Gln Ser Gly Gln Val Ala Gly Leu 
            180                 185                 190         


Leu Ala Ala Gln Ile Ala Gln Gln Leu Thr Ala Met Cys Gly Leu Gln 
        195                 200                 205             


Gln Pro Thr Pro Cys Pro Tyr Ala Ala Ala Gly Gly Val Pro His 
    210                 215                 220             


<210>  5
<211>  112
<212>  PRT
<213>  Zea mays

<400>  5

Met Arg Val Leu Leu Val Ala Leu Ala Leu Leu Ala Leu Ala Ala Ser 
1               5                   10                  15      


Ala Thr Ser Thr His Thr Ser Gly Gly Cys Gly Cys Gln Pro Pro Pro 
            20                  25                  30          


Pro Val His Leu Pro Pro Pro Val His Leu Pro Pro Pro Val His Leu 
        35                  40                  45              


Pro Pro Pro Val His Leu Pro Pro Pro Val His Leu Pro Pro Pro Val 
    50                  55                  60                  


His Leu Pro Pro Pro Val His Val Pro Pro Pro Val His Leu Pro Pro 
65                  70                  75                  80  


Pro Pro Cys His Tyr Pro Thr Gln Pro Pro Arg Pro Gln Pro His Pro 
                85                  90                  95      


Gln Pro His Pro Cys Pro Cys Gln Gln Pro His Pro Ser Pro Cys Gln 
            100                 105                 110         


<210>  6
<211>  43
<212>  PRT
<213>  Artificial sequence

<220>
<221>  source
<222>  1..43
<223>  /note= "Description of artificial sequence: Amino acid sequence of
(PPPAPA)n "

<400>  6

Ala Pro Ala Pro Pro Pro Ala Pro Ala Pro Pro Pro Ala Pro Ala Pro 
1               5                   10                  15      


Pro Pro Ala Pro Ala Pro Pro Pro Ala Pro Ala Pro Pro Pro Ala Pro 
            20                  25                  30          


Ala Pro Pro Pro Ala Pro Ala Pro Pro Pro Ala 
        35                  40              


<210>  7
<211>  43
<212>  PRT
<213>  Artificial sequence

<220>
<221>  source
<222>  1..43
<223>  /note= "Description of artificial sequence: Amino acid sequence of
(PPPEPE)n "

<400>  7

Glu Pro Ala Pro Pro Pro Glu Pro Glu Pro Pro Pro Glu Pro Glu Pro 
1               5                   10                  15      


Pro Pro Glu Pro Glu Pro Pro Pro Glu Pro Glu Pro Pro Pro Glu Pro 
            20                  25                  30          


Glu Pro Pro Pro Glu Pro Glu Pro Pro Pro Glu 
        35                  40              


<210>  8
<211>  44
<212>  PRT
<213>  Artificial sequence

<220>
<221>  source
<222>  1..44
<223>  /note= "Description of artificial sequence: Amino acid sequence of
(PPPVEL)n "

<400>  8

Val Glu Leu Pro Pro Pro Val Glu Leu Pro Pro Pro Val Glu Leu Pro 
1               5                   10                  15      


Pro Pro Val Glu Leu Pro Pro Pro Val Glu Leu Pro Pro Pro Val Glu 
            20                  25                  30          


Leu Pro Pro Pro Val Glu Val Pro Pro Pro Val Glu 
        35                  40                  


<210>  9
<211>  44
<212>  PRT
<213>  Artificial sequence

<220>
<221>  source
<222>  1..44
<223>  /note= "Description of artificial sequence: Amino acid sequence of
(PPPVAL)n "

<400>  9

Val Ala Leu Pro Pro Pro Val Ala Leu Pro Pro Pro Val Ala Leu Pro 
1               5                   10                  15      


Pro Pro Val Ala Leu Pro Pro Pro Val Ala Leu Pro Pro Pro Val Ala 
            20                  25                  30          


Leu Pro Pro Pro Val Ala Val Pro Pro Pro Val Ala 
        35                  40                  


<210>  10
<211>  44
<212>  PRT
<213>  Artificial sequence

<220>
<221>  source
<222>  1..44
<223>  /note= "Description of artificial sequence: Amino acid sequence of
(PPPVTL)n "

<400>  10

Val Thr Leu Pro Pro Pro Val Thr Leu Pro Pro Pro Val Thr Leu Pro 
1               5                   10                  15      


Pro Pro Val Thr Leu Pro Pro Pro Val Thr Leu Pro Pro Pro Val Thr 
            20                  25                  30          


Leu Pro Pro Pro Val Thr Val Pro Pro Pro Val Thr 
        35                  40                  


<210>  11
<211>  29
<212>  PRT
<213>  Artificial sequence

<220>
<221>  source
<222>  1..29
<223>  /note= "Description of artificial sequence: Amino acid sequence of
(PPPAPA)n"

<400>  11

Pro Pro Pro Ala Pro Ala Pro Pro Pro Ala Pro Ala Pro Pro Pro Ala 
1               5                   10                  15      


Pro Cys Pro Cys Pro Ala Pro Ala Pro Pro Pro Cys Pro 
            20                  25                  


<210>  12
<211>  29
<212>  PRT
<213>  Artificial sequence

<220>
<221>  source
<222>  1..29
<223>  /note= "Description of artificial sequence: Amino acid sequence of
(PPPEPE)n"

<400>  12

Pro Pro Pro Glu Pro Glu Pro Pro Pro Glu Pro Glu Pro Pro Pro Glu 
1               5                   10                  15      


Pro Cys Pro Cys Pro Glu Pro Glu Pro Pro Pro Cys Pro 
            20                  25                  


<210>  13
<211>  6
<212>  PRT
<213>  Zea mays

<400>  13

Pro Pro Pro Val His Leu 
1               5       


<210>  14
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<221>  source
<222>  1..6
<223>  /note="Description of artificial sequence: proline-rich 
       non-naturally occurring repeat sequence."

<400>  14

Pro Pro Pro Val Thr Leu 
1               5       


<210>  15
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<221>  source
<222>  1..7
<223> /note= "Description of artificial sequence: Optimum cleavage
              recognition site for Tobacco Etch Virus protease"

<400>  15

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  16
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<221>  source
<222>  1..7
<223> /note= "Description of artificial sequence: Optimum cleavage
              recognition site for Tobacco Etch Virus protease"

<400>  16

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  17
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<221>  source
<222>  1..4
<223> /note= "Description of artificial sequence: Linker sequence"

<400>  17

Gly Gly Gly Gly 
1               



<210>  18
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<221> source
<222> 1..15
<223> /note= "Description of artificial sequence: Linker sequence"

<400>  18

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  

