                         SEQUENCE LISTING

<110>  GlaxoSmithKline Biologicals sa
       Godart, Stephane
       Laanan, Amina
       Lemoine, Dominique
 
<120>  Novel compositions

<130>  VB64442

<150>  US61/422723
<151>  2010-12-14

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  723
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  M72 fusion protein

<400>  1

Met Thr Ala Ala Ser Asp Asn Phe Gln Leu Ser Gln Gly Gly Gln Gly 
1               5                   10                  15      


Phe Ala Ile Pro Ile Gly Gln Ala Met Ala Ile Ala Gly Gln Ile Arg 
            20                  25                  30          


Ser Gly Gly Gly Ser Pro Thr Val His Ile Gly Pro Thr Ala Phe Leu 
        35                  40                  45              


Gly Leu Gly Val Val Asp Asn Asn Gly Asn Gly Ala Arg Val Gln Arg 
    50                  55                  60                  


Val Val Gly Ser Ala Pro Ala Ala Ser Leu Gly Ile Ser Thr Gly Asp 
65                  70                  75                  80  


Val Ile Thr Ala Val Asp Gly Ala Pro Ile Asn Ser Ala Thr Ala Met 
                85                  90                  95      


Ala Asp Ala Leu Asn Gly His His Pro Gly Asp Val Ile Ser Val Thr 
            100                 105                 110         


Trp Gln Thr Lys Ser Gly Gly Thr Arg Thr Gly Asn Val Thr Leu Ala 
        115                 120                 125             


Glu Gly Pro Pro Ala Glu Phe Met Val Asp Phe Gly Ala Leu Pro Pro 
    130                 135                 140                 


Glu Ile Asn Ser Ala Arg Met Tyr Ala Gly Pro Gly Ser Ala Ser Leu 
145                 150                 155                 160 


Val Ala Ala Ala Gln Met Trp Asp Ser Val Ala Ser Asp Leu Phe Ser 
                165                 170                 175     


Ala Ala Ser Ala Phe Gln Ser Val Val Trp Gly Leu Thr Val Gly Ser 
            180                 185                 190         


Trp Ile Gly Ser Ser Ala Gly Leu Met Val Ala Ala Ala Ser Pro Tyr 
        195                 200                 205             


Val Ala Trp Met Ser Val Thr Ala Gly Gln Ala Glu Leu Thr Ala Ala 
    210                 215                 220                 


Gln Val Arg Val Ala Ala Ala Ala Tyr Glu Thr Ala Tyr Gly Leu Thr 
225                 230                 235                 240 


Val Pro Pro Pro Val Ile Ala Glu Asn Arg Ala Glu Leu Met Ile Leu 
                245                 250                 255     


Ile Ala Thr Asn Leu Leu Gly Gln Asn Thr Pro Ala Ile Ala Val Asn 
            260                 265                 270         


Glu Ala Glu Tyr Gly Glu Met Trp Ala Gln Asp Ala Ala Ala Met Phe 
        275                 280                 285             


Gly Tyr Ala Ala Ala Thr Ala Thr Ala Thr Ala Thr Leu Leu Pro Phe 
    290                 295                 300                 


Glu Glu Ala Pro Glu Met Thr Ser Ala Gly Gly Leu Leu Glu Gln Ala 
305                 310                 315                 320 


Ala Ala Val Glu Glu Ala Ser Asp Thr Ala Ala Ala Asn Gln Leu Met 
                325                 330                 335     


Asn Asn Val Pro Gln Ala Leu Gln Gln Leu Ala Gln Pro Thr Gln Gly 
            340                 345                 350         


Thr Thr Pro Ser Ser Lys Leu Gly Gly Leu Trp Lys Thr Val Ser Pro 
        355                 360                 365             


His Arg Ser Pro Ile Ser Asn Met Val Ser Met Ala Asn Asn His Met 
    370                 375                 380                 


Ser Met Thr Asn Ser Gly Val Ser Met Thr Asn Thr Leu Ser Ser Met 
385                 390                 395                 400 


Leu Lys Gly Phe Ala Pro Ala Ala Ala Ala Gln Ala Val Gln Thr Ala 
                405                 410                 415     


Ala Gln Asn Gly Val Arg Ala Met Ser Ser Leu Gly Ser Ser Leu Gly 
            420                 425                 430         


Ser Ser Gly Leu Gly Gly Gly Val Ala Ala Asn Leu Gly Arg Ala Ala 
        435                 440                 445             


Ser Val Gly Ser Leu Ser Val Pro Gln Ala Trp Ala Ala Ala Asn Gln 
    450                 455                 460                 


Ala Val Thr Pro Ala Ala Arg Ala Leu Pro Leu Thr Ser Leu Thr Ser 
465                 470                 475                 480 


Ala Ala Glu Arg Gly Pro Gly Gln Met Leu Gly Gly Leu Pro Val Gly 
                485                 490                 495     


Gln Met Gly Ala Arg Ala Gly Gly Gly Leu Ser Gly Val Leu Arg Val 
            500                 505                 510         


Pro Pro Arg Pro Tyr Val Met Pro His Ser Pro Ala Ala Gly Asp Ile 
        515                 520                 525             


Ala Pro Pro Ala Leu Ser Gln Asp Arg Phe Ala Asp Phe Pro Ala Leu 
    530                 535                 540                 


Pro Leu Asp Pro Ser Ala Met Val Ala Gln Val Gly Pro Gln Val Val 
545                 550                 555                 560 


Asn Ile Asn Thr Lys Leu Gly Tyr Asn Asn Ala Val Gly Ala Gly Thr 
                565                 570                 575     


Gly Ile Val Ile Asp Pro Asn Gly Val Val Leu Thr Asn Asn His Val 
            580                 585                 590         


Ile Ala Gly Ala Thr Asp Ile Asn Ala Phe Ser Val Gly Ser Gly Gln 
        595                 600                 605             


Thr Tyr Gly Val Asp Val Val Gly Tyr Asp Arg Thr Gln Asp Val Ala 
    610                 615                 620                 


Val Leu Gln Leu Arg Gly Ala Gly Gly Leu Pro Ser Ala Ala Ile Gly 
625                 630                 635                 640 


Gly Gly Val Ala Val Gly Glu Pro Val Val Ala Met Gly Asn Ser Gly 
                645                 650                 655     


Gly Gln Gly Gly Thr Pro Arg Ala Val Pro Gly Arg Val Val Ala Leu 
            660                 665                 670         


Gly Gln Thr Val Gln Ala Ser Asp Ser Leu Thr Gly Ala Glu Glu Thr 
        675                 680                 685             


Leu Asn Gly Leu Ile Gln Phe Asp Ala Ala Ile Gln Pro Gly Asp Ala 
    690                 695                 700                 


Gly Gly Pro Val Val Asn Gly Leu Gly Gln Val Val Gly Met Asn Thr 
705                 710                 715                 720 


Ala Ala Ser 
            


<210>  2
<211>  2172
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Coding sequence for M72 fusion

<400>  2
atgacggccg cgtccgataa cttccagctg tcccagggtg ggcagggatt cgccattccg       60

atcgggcagg cgatggcgat cgcgggccag atccgatcgg gtggggggtc acccaccgtt      120

catatcgggc ctaccgcctt cctcggcttg ggtgttgtcg acaacaacgg caacggcgca      180

cgagtccaac gcgtggtcgg gagcgctccg gcggcaagtc tcggcatctc caccggcgac      240

gtgatcaccg cggtcgacgg cgctccgatc aactcggcca ccgcgatggc ggacgcgctt      300

aacgggcatc atcccggtga cgtcatctcg gtgacctggc aaaccaagtc gggcggcacg      360

cgtacaggga acgtgacatt ggccgaggga cccccggccg aattcatggt ggatttcggg      420

gcgttaccac cggagatcaa ctccgcgagg atgtacgccg gcccgggttc ggcctcgctg      480

gtggccgcgg ctcagatgtg ggacagcgtg gcgagtgacc tgttttcggc cgcgtcggcg      540

tttcagtcgg tggtctgggg tctgacggtg gggtcgtgga taggttcgtc ggcgggtctg      600

atggtggcgg cggcctcgcc gtatgtggcg tggatgagcg tcaccgcggg gcaggccgag      660

ctgaccgccg cccaggtccg ggttgctgcg gcggcctacg agacggcgta tgggctgacg      720

gtgcccccgc cggtgatcgc cgagaaccgt gctgaactga tgattctgat agcgaccaac      780

ctcttggggc aaaacacccc ggcgatcgcg gtcaacgagg ccgaatacgg cgagatgtgg      840

gcccaagacg ccgccgcgat gtttggctac gccgcggcga cggcgacggc gacggcgacg      900

ttgctgccgt tcgaggaggc gccggagatg accagcgcgg gtgggctcct cgagcaggcc      960

gccgcggtcg aggaggcctc cgacaccgcc gcggcgaacc agttgatgaa caatgtgccc     1020

caggcgctgc aacagctggc ccagcccacg cagggcacca cgccttcttc caagctgggt     1080

ggcctgtgga agacggtctc gccgcatcgg tcgccgatca gcaacatggt gtcgatggcc     1140

aacaaccaca tgtcgatgac caactcgggt gtgtcgatga ccaacacctt gagctcgatg     1200

ttgaagggct ttgctccggc ggcggccgcc caggccgtgc aaaccgcggc gcaaaacggg     1260

gtccgggcga tgagctcgct gggcagctcg ctgggttctt cgggtctggg cggtggggtg     1320

gccgccaact tgggtcgggc ggcctcggtc ggttcgttgt cggtgccgca ggcctgggcc     1380

gcggccaacc aggcagtcac cccggcggcg cgggcgctgc cgctgaccag cctgaccagc     1440

gccgcggaaa gagggcccgg gcagatgctg ggcgggctgc cggtggggca gatgggcgcc     1500

agggccggtg gtgggctcag tggtgtgctg cgtgttccgc cgcgacccta tgtgatgccg     1560

cattctccgg cagccggcga tatcgccccg ccggccttgt cgcaggaccg gttcgccgac     1620

ttccccgcgc tgcccctcga cccgtccgcg atggtcgccc aagtggggcc acaggtggtc     1680

aacatcaaca ccaaactggg ctacaacaac gccgtgggcg ccgggaccgg catcgtcatc     1740

gatcccaacg gtgtcgtgct gaccaacaac cacgtgatcg cgggcgccac cgacatcaat     1800

gcgttcagcg tcggctccgg ccaaacctac ggcgtcgatg tggtcgggta tgaccgcacc     1860

caggatgtcg cggtgctgca gctgcgcggt gccggtggcc tgccgtcggc ggcgatcggt     1920

ggcggcgtcg cggttggtga gcccgtcgtc gcgatgggca acagcggtgg gcagggcgga     1980

acgccccgtg cggtgcctgg cagggtggtc gcgctcggcc aaaccgtgca ggcgtcggat     2040

tcgctgaccg gtgccgaaga gacattgaac gggttgatcc agttcgatgc cgcgatccag     2100

cccggtgatg cgggcgggcc cgtcgtcaac ggcctaggac aggtggtcgg tatgaacacg     2160

gccgcgtcct ag                                                         2172


<210>  3
<211>  725
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  M72 fusion with 2 additional his residues

<400>  3

Met His His Thr Ala Ala Ser Asp Asn Phe Gln Leu Ser Gln Gly Gly 
1               5                   10                  15      


Gln Gly Phe Ala Ile Pro Ile Gly Gln Ala Met Ala Ile Ala Gly Gln 
            20                  25                  30          


Ile Arg Ser Gly Gly Gly Ser Pro Thr Val His Ile Gly Pro Thr Ala 
        35                  40                  45              


Phe Leu Gly Leu Gly Val Val Asp Asn Asn Gly Asn Gly Ala Arg Val 
    50                  55                  60                  


Gln Arg Val Val Gly Ser Ala Pro Ala Ala Ser Leu Gly Ile Ser Thr 
65                  70                  75                  80  


Gly Asp Val Ile Thr Ala Val Asp Gly Ala Pro Ile Asn Ser Ala Thr 
                85                  90                  95      


Ala Met Ala Asp Ala Leu Asn Gly His His Pro Gly Asp Val Ile Ser 
            100                 105                 110         


Val Thr Trp Gln Thr Lys Ser Gly Gly Thr Arg Thr Gly Asn Val Thr 
        115                 120                 125             


Leu Ala Glu Gly Pro Pro Ala Glu Phe Met Val Asp Phe Gly Ala Leu 
    130                 135                 140                 


Pro Pro Glu Ile Asn Ser Ala Arg Met Tyr Ala Gly Pro Gly Ser Ala 
145                 150                 155                 160 


Ser Leu Val Ala Ala Ala Gln Met Trp Asp Ser Val Ala Ser Asp Leu 
                165                 170                 175     


Phe Ser Ala Ala Ser Ala Phe Gln Ser Val Val Trp Gly Leu Thr Val 
            180                 185                 190         


Gly Ser Trp Ile Gly Ser Ser Ala Gly Leu Met Val Ala Ala Ala Ser 
        195                 200                 205             


Pro Tyr Val Ala Trp Met Ser Val Thr Ala Gly Gln Ala Glu Leu Thr 
    210                 215                 220                 


Ala Ala Gln Val Arg Val Ala Ala Ala Ala Tyr Glu Thr Ala Tyr Gly 
225                 230                 235                 240 


Leu Thr Val Pro Pro Pro Val Ile Ala Glu Asn Arg Ala Glu Leu Met 
                245                 250                 255     


Ile Leu Ile Ala Thr Asn Leu Leu Gly Gln Asn Thr Pro Ala Ile Ala 
            260                 265                 270         


Val Asn Glu Ala Glu Tyr Gly Glu Met Trp Ala Gln Asp Ala Ala Ala 
        275                 280                 285             


Met Phe Gly Tyr Ala Ala Ala Thr Ala Thr Ala Thr Ala Thr Leu Leu 
    290                 295                 300                 


Pro Phe Glu Glu Ala Pro Glu Met Thr Ser Ala Gly Gly Leu Leu Glu 
305                 310                 315                 320 


Gln Ala Ala Ala Val Glu Glu Ala Ser Asp Thr Ala Ala Ala Asn Gln 
                325                 330                 335     


Leu Met Asn Asn Val Pro Gln Ala Leu Gln Gln Leu Ala Gln Pro Thr 
            340                 345                 350         


Gln Gly Thr Thr Pro Ser Ser Lys Leu Gly Gly Leu Trp Lys Thr Val 
        355                 360                 365             


Ser Pro His Arg Ser Pro Ile Ser Asn Met Val Ser Met Ala Asn Asn 
    370                 375                 380                 


His Met Ser Met Thr Asn Ser Gly Val Ser Met Thr Asn Thr Leu Ser 
385                 390                 395                 400 


Ser Met Leu Lys Gly Phe Ala Pro Ala Ala Ala Ala Gln Ala Val Gln 
                405                 410                 415     


Thr Ala Ala Gln Asn Gly Val Arg Ala Met Ser Ser Leu Gly Ser Ser 
            420                 425                 430         


Leu Gly Ser Ser Gly Leu Gly Gly Gly Val Ala Ala Asn Leu Gly Arg 
        435                 440                 445             


Ala Ala Ser Val Gly Ser Leu Ser Val Pro Gln Ala Trp Ala Ala Ala 
    450                 455                 460                 


Asn Gln Ala Val Thr Pro Ala Ala Arg Ala Leu Pro Leu Thr Ser Leu 
465                 470                 475                 480 


Thr Ser Ala Ala Glu Arg Gly Pro Gly Gln Met Leu Gly Gly Leu Pro 
                485                 490                 495     


Val Gly Gln Met Gly Ala Arg Ala Gly Gly Gly Leu Ser Gly Val Leu 
            500                 505                 510         


Arg Val Pro Pro Arg Pro Tyr Val Met Pro His Ser Pro Ala Ala Gly 
        515                 520                 525             


Asp Ile Ala Pro Pro Ala Leu Ser Gln Asp Arg Phe Ala Asp Phe Pro 
    530                 535                 540                 


Ala Leu Pro Leu Asp Pro Ser Ala Met Val Ala Gln Val Gly Pro Gln 
545                 550                 555                 560 


Val Val Asn Ile Asn Thr Lys Leu Gly Tyr Asn Asn Ala Val Gly Ala 
                565                 570                 575     


Gly Thr Gly Ile Val Ile Asp Pro Asn Gly Val Val Leu Thr Asn Asn 
            580                 585                 590         


His Val Ile Ala Gly Ala Thr Asp Ile Asn Ala Phe Ser Val Gly Ser 
        595                 600                 605             


Gly Gln Thr Tyr Gly Val Asp Val Val Gly Tyr Asp Arg Thr Gln Asp 
    610                 615                 620                 


Val Ala Val Leu Gln Leu Arg Gly Ala Gly Gly Leu Pro Ser Ala Ala 
625                 630                 635                 640 


Ile Gly Gly Gly Val Ala Val Gly Glu Pro Val Val Ala Met Gly Asn 
                645                 650                 655     


Ser Gly Gly Gln Gly Gly Thr Pro Arg Ala Val Pro Gly Arg Val Val 
            660                 665                 670         


Ala Leu Gly Gln Thr Val Gln Ala Ser Asp Ser Leu Thr Gly Ala Glu 
        675                 680                 685             


Glu Thr Leu Asn Gly Leu Ile Gln Phe Asp Ala Ala Ile Gln Pro Gly 
    690                 695                 700                 


Asp Ala Gly Gly Pro Val Val Asn Gly Leu Gly Gln Val Val Gly Met 
705                 710                 715                 720 


Asn Thr Ala Ala Ser 
                725 


<210>  4
<211>  2178
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Coding sequence for M72 fusion with 2 additonal his residues

<400>  4
atgcatcaca cggccgcgtc cgataacttc cagctgtccc agggtgggca gggattcgcc       60

attccgatcg ggcaggcgat ggcgatcgcg ggccagatcc gatcgggtgg ggggtcaccc      120

accgttcata tcgggcctac cgccttcctc ggcttgggtg ttgtcgacaa caacggcaac      180

ggcgcacgag tccaacgcgt ggtcgggagc gctccggcgg caagtctcgg catctccacc      240

ggcgacgtga tcaccgcggt cgacggcgct ccgatcaact cggccaccgc gatggcggac      300

gcgcttaacg ggcatcatcc cggtgacgtc atctcggtga cctggcaaac caagtcgggc      360

ggcacgcgta cagggaacgt gacattggcc gagggacccc cggccgaatt catggtggat      420

ttcggggcgt taccaccgga gatcaactcc gcgaggatgt acgccggccc gggttcggcc      480

tcgctggtgg ccgcggctca gatgtgggac agcgtggcga gtgacctgtt ttcggccgcg      540

tcggcgtttc agtcggtggt ctggggtctg acggtggggt cgtggatagg ttcgtcggcg      600

ggtctgatgg tggcggcggc ctcgccgtat gtggcgtgga tgagcgtcac cgcggggcag      660

gccgagctga ccgccgccca ggtccgggtt gctgcggcgg cctacgagac ggcgtatggg      720

ctgacggtgc ccccgccggt gatcgccgag aaccgtgctg aactgatgat tctgatagcg      780

accaacctct tggggcaaaa caccccggcg atcgcggtca acgaggccga atacggcgag      840

atgtgggccc aagacgccgc cgcgatgttt ggctacgccg cggcgacggc gacggcgacg      900

gcgacgttgc tgccgttcga ggaggcgccg gagatgacca gcgcgggtgg gctcctcgag      960

caggccgccg cggtcgagga ggcctccgac accgccgcgg cgaaccagtt gatgaacaat     1020

gtgccccagg cgctgcaaca gctggcccag cccacgcagg gcaccacgcc ttcttccaag     1080

ctgggtggcc tgtggaagac ggtctcgccg catcggtcgc cgatcagcaa catggtgtcg     1140

atggccaaca accacatgtc gatgaccaac tcgggtgtgt cgatgaccaa caccttgagc     1200

tcgatgttga agggctttgc tccggcggcg gccgcccagg ccgtgcaaac cgcggcgcaa     1260

aacggggtcc gggcgatgag ctcgctgggc agctcgctgg gttcttcggg tctgggcggt     1320

ggggtggccg ccaacttggg tcgggcggcc tcggtcggtt cgttgtcggt gccgcaggcc     1380

tgggccgcgg ccaaccaggc agtcaccccg gcggcgcggg cgctgccgct gaccagcctg     1440

accagcgccg cggaaagagg gcccgggcag atgctgggcg ggctgccggt ggggcagatg     1500

ggcgccaggg ccggtggtgg gctcagtggt gtgctgcgtg ttccgccgcg accctatgtg     1560

atgccgcatt ctccggcagc cggcgatatc gccccgccgg ccttgtcgca ggaccggttc     1620

gccgacttcc ccgcgctgcc cctcgacccg tccgcgatgg tcgcccaagt ggggccacag     1680

gtggtcaaca tcaacaccaa actgggctac aacaacgccg tgggcgccgg gaccggcatc     1740

gtcatcgatc ccaacggtgt cgtgctgacc aacaaccacg tgatcgcggg cgccaccgac     1800

atcaatgcgt tcagcgtcgg ctccggccaa acctacggcg tcgatgtggt cgggtatgac     1860

cgcacccagg atgtcgcggt gctgcagctg cgcggtgccg gtggcctgcc gtcggcggcg     1920

atcggtggcg gcgtcgcggt tggtgagccc gtcgtcgcga tgggcaacag cggtgggcag     1980

ggcggaacgc cccgtgcggt gcctggcagg gtggtcgcgc tcggccaaac cgtgcaggcg     2040

tcggattcgc tgaccggtgc cgaagagaca ttgaacgggt tgatccagtt cgatgccgcg     2100

atccagcccg gtgatgcggg cgggcccgtc gtcaacggcc taggacaggt ggtcggtatg     2160

aacacggccg cgtcctag                                                   2178


<210>  5
<211>  723
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mtb72f fusion

<400>  5

Met Thr Ala Ala Ser Asp Asn Phe Gln Leu Ser Gln Gly Gly Gln Gly 
1               5                   10                  15      


Phe Ala Ile Pro Ile Gly Gln Ala Met Ala Ile Ala Gly Gln Ile Arg 
            20                  25                  30          


Ser Gly Gly Gly Ser Pro Thr Val His Ile Gly Pro Thr Ala Phe Leu 
        35                  40                  45              


Gly Leu Gly Val Val Asp Asn Asn Gly Asn Gly Ala Arg Val Gln Arg 
    50                  55                  60                  


Val Val Gly Ser Ala Pro Ala Ala Ser Leu Gly Ile Ser Thr Gly Asp 
65                  70                  75                  80  


Val Ile Thr Ala Val Asp Gly Ala Pro Ile Asn Ser Ala Thr Ala Met 
                85                  90                  95      


Ala Asp Ala Leu Asn Gly His His Pro Gly Asp Val Ile Ser Val Thr 
            100                 105                 110         


Trp Gln Thr Lys Ser Gly Gly Thr Arg Thr Gly Asn Val Thr Leu Ala 
        115                 120                 125             


Glu Gly Pro Pro Ala Glu Phe Met Val Asp Phe Gly Ala Leu Pro Pro 
    130                 135                 140                 


Glu Ile Asn Ser Ala Arg Met Tyr Ala Gly Pro Gly Ser Ala Ser Leu 
145                 150                 155                 160 


Val Ala Ala Ala Gln Met Trp Asp Ser Val Ala Ser Asp Leu Phe Ser 
                165                 170                 175     


Ala Ala Ser Ala Phe Gln Ser Val Val Trp Gly Leu Thr Val Gly Ser 
            180                 185                 190         


Trp Ile Gly Ser Ser Ala Gly Leu Met Val Ala Ala Ala Ser Pro Tyr 
        195                 200                 205             


Val Ala Trp Met Ser Val Thr Ala Gly Gln Ala Glu Leu Thr Ala Ala 
    210                 215                 220                 


Gln Val Arg Val Ala Ala Ala Ala Tyr Glu Thr Ala Tyr Gly Leu Thr 
225                 230                 235                 240 


Val Pro Pro Pro Val Ile Ala Glu Asn Arg Ala Glu Leu Met Ile Leu 
                245                 250                 255     


Ile Ala Thr Asn Leu Leu Gly Gln Asn Thr Pro Ala Ile Ala Val Asn 
            260                 265                 270         


Glu Ala Glu Tyr Gly Glu Met Trp Ala Gln Asp Ala Ala Ala Met Phe 
        275                 280                 285             


Gly Tyr Ala Ala Ala Thr Ala Thr Ala Thr Ala Thr Leu Leu Pro Phe 
    290                 295                 300                 


Glu Glu Ala Pro Glu Met Thr Ser Ala Gly Gly Leu Leu Glu Gln Ala 
305                 310                 315                 320 


Ala Ala Val Glu Glu Ala Ser Asp Thr Ala Ala Ala Asn Gln Leu Met 
                325                 330                 335     


Asn Asn Val Pro Gln Ala Leu Gln Gln Leu Ala Gln Pro Thr Gln Gly 
            340                 345                 350         


Thr Thr Pro Ser Ser Lys Leu Gly Gly Leu Trp Lys Thr Val Ser Pro 
        355                 360                 365             


His Arg Ser Pro Ile Ser Asn Met Val Ser Met Ala Asn Asn His Met 
    370                 375                 380                 


Ser Met Thr Asn Ser Gly Val Ser Met Thr Asn Thr Leu Ser Ser Met 
385                 390                 395                 400 


Leu Lys Gly Phe Ala Pro Ala Ala Ala Ala Gln Ala Val Gln Thr Ala 
                405                 410                 415     


Ala Gln Asn Gly Val Arg Ala Met Ser Ser Leu Gly Ser Ser Leu Gly 
            420                 425                 430         


Ser Ser Gly Leu Gly Gly Gly Val Ala Ala Asn Leu Gly Arg Ala Ala 
        435                 440                 445             


Ser Val Gly Ser Leu Ser Val Pro Gln Ala Trp Ala Ala Ala Asn Gln 
    450                 455                 460                 


Ala Val Thr Pro Ala Ala Arg Ala Leu Pro Leu Thr Ser Leu Thr Ser 
465                 470                 475                 480 


Ala Ala Glu Arg Gly Pro Gly Gln Met Leu Gly Gly Leu Pro Val Gly 
                485                 490                 495     


Gln Met Gly Ala Arg Ala Gly Gly Gly Leu Ser Gly Val Leu Arg Val 
            500                 505                 510         


Pro Pro Arg Pro Tyr Val Met Pro His Ser Pro Ala Ala Gly Asp Ile 
        515                 520                 525             


Ala Pro Pro Ala Leu Ser Gln Asp Arg Phe Ala Asp Phe Pro Ala Leu 
    530                 535                 540                 


Pro Leu Asp Pro Ser Ala Met Val Ala Gln Val Gly Pro Gln Val Val 
545                 550                 555                 560 


Asn Ile Asn Thr Lys Leu Gly Tyr Asn Asn Ala Val Gly Ala Gly Thr 
                565                 570                 575     


Gly Ile Val Ile Asp Pro Asn Gly Val Val Leu Thr Asn Asn His Val 
            580                 585                 590         


Ile Ala Gly Ala Thr Asp Ile Asn Ala Phe Ser Val Gly Ser Gly Gln 
        595                 600                 605             


Thr Tyr Gly Val Asp Val Val Gly Tyr Asp Arg Thr Gln Asp Val Ala 
    610                 615                 620                 


Val Leu Gln Leu Arg Gly Ala Gly Gly Leu Pro Ser Ala Ala Ile Gly 
625                 630                 635                 640 


Gly Gly Val Ala Val Gly Glu Pro Val Val Ala Met Gly Asn Ser Gly 
                645                 650                 655     


Gly Gln Gly Gly Thr Pro Arg Ala Val Pro Gly Arg Val Val Ala Leu 
            660                 665                 670         


Gly Gln Thr Val Gln Ala Ser Asp Ser Leu Thr Gly Ala Glu Glu Thr 
        675                 680                 685             


Leu Asn Gly Leu Ile Gln Phe Asp Ala Ala Ile Gln Pro Gly Asp Ser 
    690                 695                 700                 


Gly Gly Pro Val Val Asn Gly Leu Gly Gln Val Val Gly Met Asn Thr 
705                 710                 715                 720 


Ala Ala Ser 
            


<210>  6
<211>  2172
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Coding sequence for Mtb72f fusion

<400>  6
atgacggccg cgtccgataa cttccagctg tcccagggtg ggcagggatt cgccattccg       60

atcgggcagg cgatggcgat cgcgggccag atccgatcgg gtggggggtc acccaccgtt      120

catatcgggc ctaccgcctt cctcggcttg ggtgttgtcg acaacaacgg caacggcgca      180

cgagtccaac gcgtggtcgg gagcgctccg gcggcaagtc tcggcatctc caccggcgac      240

gtgatcaccg cggtcgacgg cgctccgatc aactcggcca ccgcgatggc ggacgcgctt      300

aacgggcatc atcccggtga cgtcatctcg gtgacctggc aaaccaagtc gggcggcacg      360

cgtacaggga acgtgacatt ggccgaggga cccccggccg aattcatggt ggatttcggg      420

gcgttaccac cggagatcaa ctccgcgagg atgtacgccg gcccgggttc ggcctcgctg      480

gtggccgcgg ctcagatgtg ggacagcgtg gcgagtgacc tgttttcggc cgcgtcggcg      540

tttcagtcgg tggtctgggg tctgacggtg gggtcgtgga taggttcgtc ggcgggtctg      600

atggtggcgg cggcctcgcc gtatgtggcg tggatgagcg tcaccgcggg gcaggccgag      660

ctgaccgccg cccaggtccg ggttgctgcg gcggcctacg agacggcgta tgggctgacg      720

gtgcccccgc cggtgatcgc cgagaaccgt gctgaactga tgattctgat agcgaccaac      780

ctcttggggc aaaacacccc ggcgatcgcg gtcaacgagg ccgaatacgg cgagatgtgg      840

gcccaagacg ccgccgcgat gtttggctac gccgcggcga cggcgacggc gacggcgacg      900

ttgctgccgt tcgaggaggc gccggagatg accagcgcgg gtgggctcct cgagcaggcc      960

gccgcggtcg aggaggcctc cgacaccgcc gcggcgaacc agttgatgaa caatgtgccc     1020

caggcgctgc aacagctggc ccagcccacg cagggcacca cgccttcttc caagctgggt     1080

ggcctgtgga agacggtctc gccgcatcgg tcgccgatca gcaacatggt gtcgatggcc     1140

aacaaccaca tgtcgatgac caactcgggt gtgtcgatga ccaacacctt gagctcgatg     1200

ttgaagggct ttgctccggc ggcggccgcc caggccgtgc aaaccgcggc gcaaaacggg     1260

gtccgggcga tgagctcgct gggcagctcg ctgggttctt cgggtctggg cggtggggtg     1320

gccgccaact tgggtcgggc ggcctcggtc ggttcgttgt cggtgccgca ggcctgggcc     1380

gcggccaacc aggcagtcac cccggcggcg cgggcgctgc cgctgaccag cctgaccagc     1440

gccgcggaaa gagggcccgg gcagatgctg ggcgggctgc cggtggggca gatgggcgcc     1500

agggccggtg gtgggctcag tggtgtgctg cgtgttccgc cgcgacccta tgtgatgccg     1560

cattctccgg cagccggcga tatcgccccg ccggccttgt cgcaggaccg gttcgccgac     1620

ttccccgcgc tgcccctcga cccgtccgcg atggtcgccc aagtggggcc acaggtggtc     1680

aacatcaaca ccaaactggg ctacaacaac gccgtgggcg ccgggaccgg catcgtcatc     1740

gatcccaacg gtgtcgtgct gaccaacaac cacgtgatcg cgggcgccac cgacatcaat     1800

gcgttcagcg tcggctccgg ccaaacctac ggcgtcgatg tggtcgggta tgaccgcacc     1860

caggatgtcg cggtgctgca gctgcgcggt gccggtggcc tgccgtcggc ggcgatcggt     1920

ggcggcgtcg cggttggtga gcccgtcgtc gcgatgggca acagcggtgg gcagggcgga     1980

acgccccgtg cggtgcctgg cagggtggtc gcgctcggcc aaaccgtgca ggcgtcggat     2040

tcgctgaccg gtgccgaaga gacattgaac gggttgatcc agttcgatgc cgcgatccag     2100

cccggtgatt cgggcgggcc cgtcgtcaac ggcctaggac aggtggtcgg tatgaacacg     2160

gccgcgtcct ag                                                         2172


<210>  7
<211>  729
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mtb72f fusion with 6 additional his residues

<400>  7

Met His His His His His His Thr Ala Ala Ser Asp Asn Phe Gln Leu 
1               5                   10                  15      


Ser Gln Gly Gly Gln Gly Phe Ala Ile Pro Ile Gly Gln Ala Met Ala 
            20                  25                  30          


Ile Ala Gly Gln Ile Arg Ser Gly Gly Gly Ser Pro Thr Val His Ile 
        35                  40                  45              


Gly Pro Thr Ala Phe Leu Gly Leu Gly Val Val Asp Asn Asn Gly Asn 
    50                  55                  60                  


Gly Ala Arg Val Gln Arg Val Val Gly Ser Ala Pro Ala Ala Ser Leu 
65                  70                  75                  80  


Gly Ile Ser Thr Gly Asp Val Ile Thr Ala Val Asp Gly Ala Pro Ile 
                85                  90                  95      


Asn Ser Ala Thr Ala Met Ala Asp Ala Leu Asn Gly His His Pro Gly 
            100                 105                 110         


Asp Val Ile Ser Val Thr Trp Gln Thr Lys Ser Gly Gly Thr Arg Thr 
        115                 120                 125             


Gly Asn Val Thr Leu Ala Glu Gly Pro Pro Ala Glu Phe Met Val Asp 
    130                 135                 140                 


Phe Gly Ala Leu Pro Pro Glu Ile Asn Ser Ala Arg Met Tyr Ala Gly 
145                 150                 155                 160 


Pro Gly Ser Ala Ser Leu Val Ala Ala Ala Gln Met Trp Asp Ser Val 
                165                 170                 175     


Ala Ser Asp Leu Phe Ser Ala Ala Ser Ala Phe Gln Ser Val Val Trp 
            180                 185                 190         


Gly Leu Thr Val Gly Ser Trp Ile Gly Ser Ser Ala Gly Leu Met Val 
        195                 200                 205             


Ala Ala Ala Ser Pro Tyr Val Ala Trp Met Ser Val Thr Ala Gly Gln 
    210                 215                 220                 


Ala Glu Leu Thr Ala Ala Gln Val Arg Val Ala Ala Ala Ala Tyr Glu 
225                 230                 235                 240 


Thr Ala Tyr Gly Leu Thr Val Pro Pro Pro Val Ile Ala Glu Asn Arg 
                245                 250                 255     


Ala Glu Leu Met Ile Leu Ile Ala Thr Asn Leu Leu Gly Gln Asn Thr 
            260                 265                 270         


Pro Ala Ile Ala Val Asn Glu Ala Glu Tyr Gly Glu Met Trp Ala Gln 
        275                 280                 285             


Asp Ala Ala Ala Met Phe Gly Tyr Ala Ala Ala Thr Ala Thr Ala Thr 
    290                 295                 300                 


Ala Thr Leu Leu Pro Phe Glu Glu Ala Pro Glu Met Thr Ser Ala Gly 
305                 310                 315                 320 


Gly Leu Leu Glu Gln Ala Ala Ala Val Glu Glu Ala Ser Asp Thr Ala 
                325                 330                 335     


Ala Ala Asn Gln Leu Met Asn Asn Val Pro Gln Ala Leu Gln Gln Leu 
            340                 345                 350         


Ala Gln Pro Thr Gln Gly Thr Thr Pro Ser Ser Lys Leu Gly Gly Leu 
        355                 360                 365             


Trp Lys Thr Val Ser Pro His Arg Ser Pro Ile Ser Asn Met Val Ser 
    370                 375                 380                 


Met Ala Asn Asn His Met Ser Met Thr Asn Ser Gly Val Ser Met Thr 
385                 390                 395                 400 


Asn Thr Leu Ser Ser Met Leu Lys Gly Phe Ala Pro Ala Ala Ala Ala 
                405                 410                 415     


Gln Ala Val Gln Thr Ala Ala Gln Asn Gly Val Arg Ala Met Ser Ser 
            420                 425                 430         


Leu Gly Ser Ser Leu Gly Ser Ser Gly Leu Gly Gly Gly Val Ala Ala 
        435                 440                 445             


Asn Leu Gly Arg Ala Ala Ser Val Gly Ser Leu Ser Val Pro Gln Ala 
    450                 455                 460                 


Trp Ala Ala Ala Asn Gln Ala Val Thr Pro Ala Ala Arg Ala Leu Pro 
465                 470                 475                 480 


Leu Thr Ser Leu Thr Ser Ala Ala Glu Arg Gly Pro Gly Gln Met Leu 
                485                 490                 495     


Gly Gly Leu Pro Val Gly Gln Met Gly Ala Arg Ala Gly Gly Gly Leu 
            500                 505                 510         


Ser Gly Val Leu Arg Val Pro Pro Arg Pro Tyr Val Met Pro His Ser 
        515                 520                 525             


Pro Ala Ala Gly Asp Ile Ala Pro Pro Ala Leu Ser Gln Asp Arg Phe 
    530                 535                 540                 


Ala Asp Phe Pro Ala Leu Pro Leu Asp Pro Ser Ala Met Val Ala Gln 
545                 550                 555                 560 


Val Gly Pro Gln Val Val Asn Ile Asn Thr Lys Leu Gly Tyr Asn Asn 
                565                 570                 575     


Ala Val Gly Ala Gly Thr Gly Ile Val Ile Asp Pro Asn Gly Val Val 
            580                 585                 590         


Leu Thr Asn Asn His Val Ile Ala Gly Ala Thr Asp Ile Asn Ala Phe 
        595                 600                 605             


Ser Val Gly Ser Gly Gln Thr Tyr Gly Val Asp Val Val Gly Tyr Asp 
    610                 615                 620                 


Arg Thr Gln Asp Val Ala Val Leu Gln Leu Arg Gly Ala Gly Gly Leu 
625                 630                 635                 640 


Pro Ser Ala Ala Ile Gly Gly Gly Val Ala Val Gly Glu Pro Val Val 
                645                 650                 655     


Ala Met Gly Asn Ser Gly Gly Gln Gly Gly Thr Pro Arg Ala Val Pro 
            660                 665                 670         


Gly Arg Val Val Ala Leu Gly Gln Thr Val Gln Ala Ser Asp Ser Leu 
        675                 680                 685             


Thr Gly Ala Glu Glu Thr Leu Asn Gly Leu Ile Gln Phe Asp Ala Ala 
    690                 695                 700                 


Ile Gln Pro Gly Asp Ser Gly Gly Pro Val Val Asn Gly Leu Gly Gln 
705                 710                 715                 720 


Val Val Gly Met Asn Thr Ala Ala Ser 
                725                 


<210>  8
<211>  2190
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Coding sequence for M72 fusion with 6 additonal his residues

<400>  8
atgcatcacc atcaccatca cacggccgcg tccgataact tccagctgtc ccagggtggg       60

cagggattcg ccattccgat cgggcaggcg atggcgatcg cgggccagat ccgatcgggt      120

ggggggtcac ccaccgttca tatcgggcct accgccttcc tcggcttggg tgttgtcgac      180

aacaacggca acggcgcacg agtccaacgc gtggtcggga gcgctccggc ggcaagtctc      240

ggcatctcca ccggcgacgt gatcaccgcg gtcgacggcg ctccgatcaa ctcggccacc      300

gcgatggcgg acgcgcttaa cgggcatcat cccggtgacg tcatctcggt gacctggcaa      360

accaagtcgg gcggcacgcg tacagggaac gtgacattgg ccgagggacc cccggccgaa      420

ttcatggtgg atttcggggc gttaccaccg gagatcaact ccgcgaggat gtacgccggc      480

ccgggttcgg cctcgctggt ggccgcggct cagatgtggg acagcgtggc gagtgacctg      540

ttttcggccg cgtcggcgtt tcagtcggtg gtctggggtc tgacggtggg gtcgtggata      600

ggttcgtcgg cgggtctgat ggtggcggcg gcctcgccgt atgtggcgtg gatgagcgtc      660

accgcggggc aggccgagct gaccgccgcc caggtccggg ttgctgcggc ggcctacgag      720

acggcgtatg ggctgacggt gcccccgccg gtgatcgccg agaaccgtgc tgaactgatg      780

attctgatag cgaccaacct cttggggcaa aacaccccgg cgatcgcggt caacgaggcc      840

gaatacggcg agatgtgggc ccaagacgcc gccgcgatgt ttggctacgc cgcggcgacg      900

gcgacggcga cggcgacgtt gctgccgttc gaggaggcgc cggagatgac cagcgcgggt      960

gggctcctcg agcaggccgc cgcggtcgag gaggcctccg acaccgccgc ggcgaaccag     1020

ttgatgaaca atgtgcccca ggcgctgcaa cagctggccc agcccacgca gggcaccacg     1080

ccttcttcca agctgggtgg cctgtggaag acggtctcgc cgcatcggtc gccgatcagc     1140

aacatggtgt cgatggccaa caaccacatg tcgatgacca actcgggtgt gtcgatgacc     1200

aacaccttga gctcgatgtt gaagggcttt gctccggcgg cggccgccca ggccgtgcaa     1260

accgcggcgc aaaacggggt ccgggcgatg agctcgctgg gcagctcgct gggttcttcg     1320

ggtctgggcg gtggggtggc cgccaacttg ggtcgggcgg cctcggtcgg ttcgttgtcg     1380

gtgccgcagg cctgggccgc ggccaaccag gcagtcaccc cggcggcgcg ggcgctgccg     1440

ctgaccagcc tgaccagcgc cgcggaaaga gggcccgggc agatgctggg cgggctgccg     1500

gtggggcaga tgggcgccag ggccggtggt gggctcagtg gtgtgctgcg tgttccgccg     1560

cgaccctatg tgatgccgca ttctccggca gccggcgata tcgccccgcc ggccttgtcg     1620

caggaccggt tcgccgactt ccccgcgctg cccctcgacc cgtccgcgat ggtcgcccaa     1680

gtggggccac aggtggtcaa catcaacacc aaactgggct acaacaacgc cgtgggcgcc     1740

gggaccggca tcgtcatcga tcccaacggt gtcgtgctga ccaacaacca cgtgatcgcg     1800

ggcgccaccg acatcaatgc gttcagcgtc ggctccggcc aaacctacgg cgtcgatgtg     1860

gtcgggtatg accgcaccca ggatgtcgcg gtgctgcagc tgcgcggtgc cggtggcctg     1920

ccgtcggcgg cgatcggtgg cggcgtcgcg gttggtgagc ccgtcgtcgc gatgggcaac     1980

agcggtgggc agggcggaac gccccgtgcg gtgcctggca gggtggtcgc gctcggccaa     2040

accgtgcagg cgtcggattc gctgaccggt gccgaagaga cattgaacgg gttgatccag     2100

ttcgatgccg cgatccagcc cggtgattcg ggcgggcccg tcgtcaacgg cctaggacag     2160

gtggtcggta tgaacacggc cgcgtcctag                                      2190


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cpg Oligo 1 - CpG 1826

<400>  9
tccatgacgt tcctgacgtt                                                   20


<210>  10
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CpG Oligo 2 - CpG 1758

<400>  10
tctcccagcg tgcgccat                                                     18


<210>  11
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CpG Oligo 3

<400>  11
accgatgacg tcgccggtga cggcaccacg                                        30


<210>  12
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CpG Oligo 4 - CpG 2006

<400>  12
tcgtcgtttt gtcgttttgt cgtt                                              24


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CpG Oligo 5 - CpG 1686

<400>  13
tccatgacgt tcctgatgct                                                   20


