                         SEQUENCE LISTING

<110>  Jessen, Holly J
       Li, Jian
 
<120>  COMPOSITIONS AND METHODS FOR INCREASED ETHANOL TITER FROM BIOMASS

<130>  33449.8054.WO00

<140>  
<141>  November 22, 2011

<150>  61/416,169
<151>  November 22, 2010

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  3179
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1052)..(2182)

<400>  1
gatttggacc tacaaggtgc tgtaaagagt atgaacactt ctggggagga ggaatggaac       60

agtgatgacg atgatgatga agaaagtgac gaaagtaacg aaagtgatta ctattcttac      120

gatgaaggcg aagaaacaga tgatagtgag ggagcccaag agggagagga agacgaaaat      180

gaacgaatca ttgaagctct aagtagtggt gttggtgaac tcaagatgga ctctttaggt      240

aattatattc ttgaatagtt gtgtaaagcg aatatgcaaa tagatttgtt ttataattat      300

gcatctcttt gaaagaggtt tagaggcaaa gttcttgcat acaatattgt gattgtttta      360

atgtcattct tgattttcat aaagagatta aaaaaaaaaa aaaaaaactt ataaaattga      420

gtagaaccat ttatatataa gacaaagatt gtctgtatta gtcctcaaca cactaaacct      480

tacatactta gggtaaattt gctaatagag tgatatgttc atgagaactc caacgacaac      540

acaaccacct atttgcacaa caaacaccat tgtcgcacgc tgcgcgccct agaagtagaa      600

agaaagggaa atgacattaa gagaatcata ccccgtgccc gtaacgccga aaaaatcaca      660

ccccgtcccc cacaccttaa aacctcaacc gcttaacacc gccacaccct ttctctttat      720

aaacgccgtt tgcattactc attcttctta taaaccgcac cccccaaaac gcggaatagc      780

ttcaaccccc caatcagata tgagtttccc gggaaacccg cttttcccga cagccccaca      840

aggggttggt ctataaaaga ggacgttttc cccgtcatcg agattgaaga ttcttacagg      900

cccatttatt caaattggag ttgattcttc ttgtctttac tttctttctc tctttttctt      960

ccttttttaa tattatcttt tgtcaagcct ggttccctaa gttgaactct cttttcttgt     1020

gatcctccta tatagatacg ccttgccaaa t atg ttt gca tca acc ttc aga        1072
                                   Met Phe Ala Ser Thr Phe Arg            
                                   1               5                      

agt caa gct gta aga gct gca aga ttt act aga ttc caa tcc act ttt       1120
Ser Gln Ala Val Arg Ala Ala Arg Phe Thr Arg Phe Gln Ser Thr Phe           
        10                  15                  20                        

gcc att cct gag aag caa atg ggt gtt atc ttt gaa act cat ggt ggt       1168
Ala Ile Pro Glu Lys Gln Met Gly Val Ile Phe Glu Thr His Gly Gly           
    25                  30                  35                            

cct tta caa tac aag gaa att cca gtt cca aaa cca aaa cca act gaa       1216
Pro Leu Gln Tyr Lys Glu Ile Pro Val Pro Lys Pro Lys Pro Thr Glu           
40                  45                  50                  55            

att tta atc aat gtt aaa tac tct ggt gtc tgc cat acc gat tta cac       1264
Ile Leu Ile Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu His           
                60                  65                  70                

gca tgg aaa ggt gac tgg cca tta cca gca aag tta ccc cta gtt ggt       1312
Ala Trp Lys Gly Asp Trp Pro Leu Pro Ala Lys Leu Pro Leu Val Gly           
            75                  80                  85                    

ggt cac gaa ggt gcg ggc att gtt gtt gcg aaa ggt tct gca gtt acc       1360
Gly His Glu Gly Ala Gly Ile Val Val Ala Lys Gly Ser Ala Val Thr           
        90                  95                  100                       

aac ttt gag att ggc gat tat gct ggt att aag tgg tta aac ggt tca       1408
Asn Phe Glu Ile Gly Asp Tyr Ala Gly Ile Lys Trp Leu Asn Gly Ser           
    105                 110                 115                           

tgt atg tca tgt gaa ttc tgt gaa caa ggt gat gaa tct aac tgt gaa       1456
Cys Met Ser Cys Glu Phe Cys Glu Gln Gly Asp Glu Ser Asn Cys Glu           
120                 125                 130                 135           

cat gcc gat ttg agt ggt tat act cat gat ggt tct ttc caa caa tat       1504
His Ala Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Gln Tyr           
                140                 145                 150               

gcc act gct gac gct att caa gct gca aag atc cca aag ggt acc gac       1552
Ala Thr Ala Asp Ala Ile Gln Ala Ala Lys Ile Pro Lys Gly Thr Asp           
            155                 160                 165                   

tta tct gaa gtt gcg cca att tta tgt gct ggt gtt act gtc tat aaa       1600
Leu Ser Glu Val Ala Pro Ile Leu Cys Ala Gly Val Thr Val Tyr Lys           
        170                 175                 180                       

gct ttg aaa act gct gat tta aga gca ggt caa tgg gtt gcg att tct       1648
Ala Leu Lys Thr Ala Asp Leu Arg Ala Gly Gln Trp Val Ala Ile Ser           
    185                 190                 195                           

ggt gcc gct ggt ggt cta ggt tct ctt gct gtc caa tat gca aag gca       1696
Gly Ala Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys Ala           
200                 205                 210                 215           

atg ggt cta aga gtt tta ggt atc gat ggt ggt gaa ggt aaa aag gaa       1744
Met Gly Leu Arg Val Leu Gly Ile Asp Gly Gly Glu Gly Lys Lys Glu           
                220                 225                 230               

ctt ttt gaa caa tgt ggt ggt gat gtg ttt atc gat ttc acc aga tac       1792
Leu Phe Glu Gln Cys Gly Gly Asp Val Phe Ile Asp Phe Thr Arg Tyr           
            235                 240                 245                   

cca aga gat gca cct gaa aag atg gtt gct gat att aag gct gca act       1840
Pro Arg Asp Ala Pro Glu Lys Met Val Ala Asp Ile Lys Ala Ala Thr           
        250                 255                 260                       

aac ggt ttg ggt cca cac ggt gtt atc aat gtc tct gtc tcc cca gct       1888
Asn Gly Leu Gly Pro His Gly Val Ile Asn Val Ser Val Ser Pro Ala           
    265                 270                 275                           

gct atc tct caa tca tgt gac tat gtt aga gca act ggt aag gtt gtc       1936
Ala Ile Ser Gln Ser Cys Asp Tyr Val Arg Ala Thr Gly Lys Val Val           
280                 285                 290                 295           

ctt gtc ggt atg cca tct ggt gct gtc tgt aag tct gat gtc ttc act       1984
Leu Val Gly Met Pro Ser Gly Ala Val Cys Lys Ser Asp Val Phe Thr           
                300                 305                 310               

cat gtt gtt aaa tcc tta caa att aaa ggt tct tat gtt ggt aac aga       2032
His Val Val Lys Ser Leu Gln Ile Lys Gly Ser Tyr Val Gly Asn Arg           
            315                 320                 325                   

gca gat acc aga gaa gct ttg gaa ttc ttt aat gaa ggt aag gtc aga       2080
Ala Asp Thr Arg Glu Ala Leu Glu Phe Phe Asn Glu Gly Lys Val Arg           
        330                 335                 340                       

tct cca atc aag gtt gtc cca tta tct act tta cct gaa att tac gaa       2128
Ser Pro Ile Lys Val Val Pro Leu Ser Thr Leu Pro Glu Ile Tyr Glu           
    345                 350                 355                           

ttg atg gag caa ggt aag att tta ggt aga tac gtt gtt gat act tct       2176
Leu Met Glu Gln Gly Lys Ile Leu Gly Arg Tyr Val Val Asp Thr Ser           
360                 365                 370                 375           

aaa taa tgaagatgaa gaaaacagca aactttttat gactaccccc aaccatctaa        2232
Lys                                                                       
                                                                          

cgatttatga tctatatata gctttctaga acatccattt atttattcac ttactcatgt     2292

atttatatta tataatacaa aataactaat tacaatgtgt acattttttt ttttcattac     2352

cataatgtat gcgttgagcc tcttgcacct tctttattag gaaatcagtt gaaaaatttc     2412

cggattgtct ttattattgg cccatttttt tttggtcaca cctttatttt tgtacacttc     2472

tcgggcaaag caaaaactat agtaccggat aggcctttat aaaactccag tgtgtatgat     2532

tttagttggt gtgccatcta cacgttctct tagtttcttt atcatgtcac agaaagcaag     2592

catgcaaacc cttacaaaaa ataacaacat acaaatgcct aaacaactgg actataatga     2652

tggtgagtca gttacgaaaa gagcaagtgg gttaatacga tttcgtaagg gacagtctga     2712

ggaagactac aattttcaaa aggagcagtt ctggtccacg ggtcctttag tacagaatca     2772

cacatttgtg actgaatttg ttgaaaagtt tattgaaaac acaattagtg aagattattc     2832

aatcacagat agatcgaaaa tagaacgtga aacaatcata cacggattgg agaagctgta     2892

ttttcaaagg gaatatgagc gatgtctaaa agatgttcaa ctattgaagg acaatatcga     2952

taagttcaat cctaatttgg atcttaatga aaagaattta taatgagctg aattatattt     3012

cttggatgtg catcaaaaag atccatgaga gtaacgaaaa gaaactgggg gaaatctaat     3072

aatttacaat ttcaatatac acttctatat cctttaatgt aatggcttta taaataaaca     3132

cgaacttcta cagcaccgac gtttcttttt cttaccagct cctcttc                   3179


<210>  2
<211>  376
<212>  PRT
<213>  Issatchenkia orientalis

<400>  2

Met Phe Ala Ser Thr Phe Arg Ser Gln Ala Val Arg Ala Ala Arg Phe 
1               5                   10                  15      


Thr Arg Phe Gln Ser Thr Phe Ala Ile Pro Glu Lys Gln Met Gly Val 
            20                  25                  30          


Ile Phe Glu Thr His Gly Gly Pro Leu Gln Tyr Lys Glu Ile Pro Val 
        35                  40                  45              


Pro Lys Pro Lys Pro Thr Glu Ile Leu Ile Asn Val Lys Tyr Ser Gly 
    50                  55                  60                  


Val Cys His Thr Asp Leu His Ala Trp Lys Gly Asp Trp Pro Leu Pro 
65                  70                  75                  80  


Ala Lys Leu Pro Leu Val Gly Gly His Glu Gly Ala Gly Ile Val Val 
                85                  90                  95      


Ala Lys Gly Ser Ala Val Thr Asn Phe Glu Ile Gly Asp Tyr Ala Gly 
            100                 105                 110         


Ile Lys Trp Leu Asn Gly Ser Cys Met Ser Cys Glu Phe Cys Glu Gln 
        115                 120                 125             


Gly Asp Glu Ser Asn Cys Glu His Ala Asp Leu Ser Gly Tyr Thr His 
    130                 135                 140                 


Asp Gly Ser Phe Gln Gln Tyr Ala Thr Ala Asp Ala Ile Gln Ala Ala 
145                 150                 155                 160 


Lys Ile Pro Lys Gly Thr Asp Leu Ser Glu Val Ala Pro Ile Leu Cys 
                165                 170                 175     


Ala Gly Val Thr Val Tyr Lys Ala Leu Lys Thr Ala Asp Leu Arg Ala 
            180                 185                 190         


Gly Gln Trp Val Ala Ile Ser Gly Ala Ala Gly Gly Leu Gly Ser Leu 
        195                 200                 205             


Ala Val Gln Tyr Ala Lys Ala Met Gly Leu Arg Val Leu Gly Ile Asp 
    210                 215                 220                 


Gly Gly Glu Gly Lys Lys Glu Leu Phe Glu Gln Cys Gly Gly Asp Val 
225                 230                 235                 240 


Phe Ile Asp Phe Thr Arg Tyr Pro Arg Asp Ala Pro Glu Lys Met Val 
                245                 250                 255     


Ala Asp Ile Lys Ala Ala Thr Asn Gly Leu Gly Pro His Gly Val Ile 
            260                 265                 270         


Asn Val Ser Val Ser Pro Ala Ala Ile Ser Gln Ser Cys Asp Tyr Val 
        275                 280                 285             


Arg Ala Thr Gly Lys Val Val Leu Val Gly Met Pro Ser Gly Ala Val 
    290                 295                 300                 


Cys Lys Ser Asp Val Phe Thr His Val Val Lys Ser Leu Gln Ile Lys 
305                 310                 315                 320 


Gly Ser Tyr Val Gly Asn Arg Ala Asp Thr Arg Glu Ala Leu Glu Phe 
                325                 330                 335     


Phe Asn Glu Gly Lys Val Arg Ser Pro Ile Lys Val Val Pro Leu Ser 
            340                 345                 350         


Thr Leu Pro Glu Ile Tyr Glu Leu Met Glu Gln Gly Lys Ile Leu Gly 
        355                 360                 365             


Arg Tyr Val Val Asp Thr Ser Lys 
    370                 375     


<210>  3
<211>  3134
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1001)..(2134)

<400>  3
atgtatttgg agatttcgaa aagagtttgt atagagtctg taattgggtg tgtatttcaa       60

gacccacttt aaactgcgcc attaggagag ggagaggggg gggggggggg ggaagacggt      120

gaagtgtata caggatcgaa gaatagaagt tgtgtgtgtg ttttattacc cgtttcgatg      180

ggattcccag aagtggatac tatactgtct gcaatgcact acactctaaa aaagtattat      240

acattaccat acattagcaa atcaccaata ctctgcactg tttcagtgtg tgcacattgc      300

tacccaattg ggaaattgca gggaaaatga gaccccccct ccattccgta ttacgtaaga      360

caatatcagg gctgccgaat tcggcagaaa agccgagccg gccgagtcct cttgcacgga      420

gtgtgtccga aaagggcagc tctgcagtgg gggagaggag gtcgcacgtc tatgcggtgt      480

tggcatggcc tgtgcgtgta cctgtcccct ccctgggcat cccccactgc gcgccttctc      540

cattgggcgc tgcgggcact ccgcgccgtt aatacaggag gggggggggg aaagcttaag      600

attagagcgg gtacagtcag tgggtgtatt gaccccattt ctgtcagtat aaaccccccg      660

ttgagccgcc ggtttggttg tttatggata aaattttttt tccccgcatg gagaagattg      720

agggggagaa ggaatgggaa aaaggccaga gccatctcca cagcggaatc cgaccgttaa      780

tggggtgaaa cacccccacc aggtagagca ggaagaatgg ggaaacaagg tggagagatg      840

gtcattgttg ggaatagtgg gaaaatgagg gggaagagaa tgactataaa atgggaaggg      900

ggtccaagtt atccaagcag tccatttaga gaagggaaaa taaagctata gatagaaacc      960

aaccaaacaa ccaaacaatt aaacaaacaa ttaaacgaac atg tta tcc aag acc       1015
                                            Met Leu Ser Lys Thr           
                                            1               5             

atc act gct gca ttg agg ggc aat aca act cgt act gca ttc aga atc       1063
Ile Thr Ala Ala Leu Arg Gly Asn Thr Thr Arg Thr Ala Phe Arg Ile           
                10                  15                  20                

aat gcc att aga agt tta gcg atc cca gct att cca gag aca caa aag       1111
Asn Ala Ile Arg Ser Leu Ala Ile Pro Ala Ile Pro Glu Thr Gln Lys           
            25                  30                  35                    

ggt gtt atc ttt tat gag aac gga ggt gaa cta ttt tac aag gac att       1159
Gly Val Ile Phe Tyr Glu Asn Gly Gly Glu Leu Phe Tyr Lys Asp Ile           
        40                  45                  50                        

cca gtt cca aag cca aag cca aat gag att ttg gtg aat gtc aag tat       1207
Pro Val Pro Lys Pro Lys Pro Asn Glu Ile Leu Val Asn Val Lys Tyr           
    55                  60                  65                            

tct ggt gtt tgt cat acc gat tta cac gca tgg aaa ggt gac tgg cct       1255
Ser Gly Val Cys His Thr Asp Leu His Ala Trp Lys Gly Asp Trp Pro           
70                  75                  80                  85            

ttg gcg acc aag ttg cca ttg gtt ggt gga cat gaa ggt gcc gga gtt       1303
Leu Ala Thr Lys Leu Pro Leu Val Gly Gly His Glu Gly Ala Gly Val           
                90                  95                  100               

gtt gtt gct aag ggg gac aat gtc acc aac ttt gaa att ggc gat tat       1351
Val Val Ala Lys Gly Asp Asn Val Thr Asn Phe Glu Ile Gly Asp Tyr           
            105                 110                 115                   

gcc ggt atc aag tgg ttg aat ggt tca tgt atg ggg tgt gaa ttt tgc       1399
Ala Gly Ile Lys Trp Leu Asn Gly Ser Cys Met Gly Cys Glu Phe Cys           
        120                 125                 130                       

caa caa ggt gca gag cca aac tgt cca cag gcc gac ttg agt ggt tac       1447
Gln Gln Gly Ala Glu Pro Asn Cys Pro Gln Ala Asp Leu Ser Gly Tyr           
    135                 140                 145                           

acc cat gac ggg tcc ttt caa caa tat gcc act gcc gat gct gtt cag       1495
Thr His Asp Gly Ser Phe Gln Gln Tyr Ala Thr Ala Asp Ala Val Gln           
150                 155                 160                 165           

gca gcc aag att cct cag ggc act gat ttg gct caa gtt gcg cca att       1543
Ala Ala Lys Ile Pro Gln Gly Thr Asp Leu Ala Gln Val Ala Pro Ile           
                170                 175                 180               

tta tgt gca ggt att act gtc tat aag gct tta aag act gca gaa tta       1591
Leu Cys Ala Gly Ile Thr Val Tyr Lys Ala Leu Lys Thr Ala Glu Leu           
            185                 190                 195                   

aga cca ggt caa tgg gtt gcc att tct ggt gct gct gga ggt tta ggt       1639
Arg Pro Gly Gln Trp Val Ala Ile Ser Gly Ala Ala Gly Gly Leu Gly           
        200                 205                 210                       

tct ctt gct gtt caa tat gcc aag gcc atg ggt ttg aga gtt ttg ggt       1687
Ser Leu Ala Val Gln Tyr Ala Lys Ala Met Gly Leu Arg Val Leu Gly           
    215                 220                 225                           

att gat ggt ggt gag gag aag ggc aag ttt gca aag tct ctt gga gct       1735
Ile Asp Gly Gly Glu Glu Lys Gly Lys Phe Ala Lys Ser Leu Gly Ala           
230                 235                 240                 245           

gaa gtt ttc att gat ttc acc aaa tcc aag gac att gtc aag gat atc       1783
Glu Val Phe Ile Asp Phe Thr Lys Ser Lys Asp Ile Val Lys Asp Ile           
                250                 255                 260               

caa gag gcc acc aat ggt ggt cca cat ggt gtc att aat gtt tct gtt       1831
Gln Glu Ala Thr Asn Gly Gly Pro His Gly Val Ile Asn Val Ser Val           
            265                 270                 275                   

tct cca gct gct att tct caa agt acc cag tat gtc aga acc ttg ggt       1879
Ser Pro Ala Ala Ile Ser Gln Ser Thr Gln Tyr Val Arg Thr Leu Gly           
        280                 285                 290                       

aag gtt gtc ctt gtt gga tta cca gcg cat gct gta tgc gag tct tcg       1927
Lys Val Val Leu Val Gly Leu Pro Ala His Ala Val Cys Glu Ser Ser           
    295                 300                 305                           

gtt ttc gac cat gtt gtc aag tcg att caa att aga ggc tct tat gtt       1975
Val Phe Asp His Val Val Lys Ser Ile Gln Ile Arg Gly Ser Tyr Val           
310                 315                 320                 325           

ggt aac agg gaa gat act agt gag gct att gat ttt ttc acc agg ggt       2023
Gly Asn Arg Glu Asp Thr Ser Glu Ala Ile Asp Phe Phe Thr Arg Gly           
                330                 335                 340               

tta gtg aag tca cca att aag att gtt ggt ttg agt gag ttg cca aag       2071
Leu Val Lys Ser Pro Ile Lys Ile Val Gly Leu Ser Glu Leu Pro Lys           
            345                 350                 355                   

atc tat gaa ttg atg gag caa ggt aag att tta ggc aga tat gtt gtt       2119
Ile Tyr Glu Leu Met Glu Gln Gly Lys Ile Leu Gly Arg Tyr Val Val           
        360                 365                 370                       

gac act tcg aaa tga tgggctgact tgggtgtact ggtgtgacgt ttttatgtgt       2174
Asp Thr Ser Lys                                                           
    375                                                                   

atattgatat gcatggggga tgtatagtga tgaggagtag agtatataac gaaatgaaat     2234

gaaataatat gatatgataa gataagatga gatcaaatac gataatataa gatgcgacat     2294

gaggagttca atgtagcata ctacacgatg ctgcagtaca actctgatac gctagactat     2354

actatacaaa actgtagtac actatacgtt agtgtagtat ccagaaacaa cactgcttta     2414

tagtacaata caactctata atactatagt atactatgcc aaaccacgta ataccataat     2474

atgctccacg acatggtaca atgtgctata cttcatacta ttataccata tatactccga     2534

tatattattg atatactatt ttatactata ataccatacc acacaacact acattacaac     2594

gagcaacctt accataaatg tcagttatgt ggcccggaga ctctctcgag gagcgtgttc     2654

acctcgttgt agacgttctg cacatcctct ccgagcaggg cacgtgctcc catagtggga     2714

ggggcctctt ccaagggcga cccgcggcgc cccgcaccaa gaagcgcctg ttccttgagc     2774

gcatgtgcaa tattgagaag ggtgtctatg ctgcgaagaa cggtgtctgt gtcggcagca     2834

gcagcagcgg cgtctgctcc ctgggcggaa cgtgtcttcc ccgctaaggg gagcacagca     2894

agaatatcat gtaatgcagc aagagcattc tgagttgaag tatcgatttt cgatgccata     2954

ttgtatgtgt attgtattaa gtgtgtattg tcttaagtgt gtaagagaca tttatttgtg     3014

tcaacaatag cgacgccact gaaaacctca aatatcgtat ttattaatcc ccttcccccc     3074

agcgcagatc gtcccgtcga tttctattgt ttgggcatta tcagcgacgc gacggcgacg     3134


<210>  4
<211>  377
<212>  PRT
<213>  Issatchenkia orientalis

<400>  4

Met Leu Ser Lys Thr Ile Thr Ala Ala Leu Arg Gly Asn Thr Thr Arg 
1               5                   10                  15      


Thr Ala Phe Arg Ile Asn Ala Ile Arg Ser Leu Ala Ile Pro Ala Ile 
            20                  25                  30          


Pro Glu Thr Gln Lys Gly Val Ile Phe Tyr Glu Asn Gly Gly Glu Leu 
        35                  40                  45              


Phe Tyr Lys Asp Ile Pro Val Pro Lys Pro Lys Pro Asn Glu Ile Leu 
    50                  55                  60                  


Val Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu His Ala Trp 
65                  70                  75                  80  


Lys Gly Asp Trp Pro Leu Ala Thr Lys Leu Pro Leu Val Gly Gly His 
                85                  90                  95      


Glu Gly Ala Gly Val Val Val Ala Lys Gly Asp Asn Val Thr Asn Phe 
            100                 105                 110         


Glu Ile Gly Asp Tyr Ala Gly Ile Lys Trp Leu Asn Gly Ser Cys Met 
        115                 120                 125             


Gly Cys Glu Phe Cys Gln Gln Gly Ala Glu Pro Asn Cys Pro Gln Ala 
    130                 135                 140                 


Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Gln Tyr Ala Thr 
145                 150                 155                 160 


Ala Asp Ala Val Gln Ala Ala Lys Ile Pro Gln Gly Thr Asp Leu Ala 
                165                 170                 175     


Gln Val Ala Pro Ile Leu Cys Ala Gly Ile Thr Val Tyr Lys Ala Leu 
            180                 185                 190         


Lys Thr Ala Glu Leu Arg Pro Gly Gln Trp Val Ala Ile Ser Gly Ala 
        195                 200                 205             


Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys Ala Met Gly 
    210                 215                 220                 


Leu Arg Val Leu Gly Ile Asp Gly Gly Glu Glu Lys Gly Lys Phe Ala 
225                 230                 235                 240 


Lys Ser Leu Gly Ala Glu Val Phe Ile Asp Phe Thr Lys Ser Lys Asp 
                245                 250                 255     


Ile Val Lys Asp Ile Gln Glu Ala Thr Asn Gly Gly Pro His Gly Val 
            260                 265                 270         


Ile Asn Val Ser Val Ser Pro Ala Ala Ile Ser Gln Ser Thr Gln Tyr 
        275                 280                 285             


Val Arg Thr Leu Gly Lys Val Val Leu Val Gly Leu Pro Ala His Ala 
    290                 295                 300                 


Val Cys Glu Ser Ser Val Phe Asp His Val Val Lys Ser Ile Gln Ile 
305                 310                 315                 320 


Arg Gly Ser Tyr Val Gly Asn Arg Glu Asp Thr Ser Glu Ala Ile Asp 
                325                 330                 335     


Phe Phe Thr Arg Gly Leu Val Lys Ser Pro Ile Lys Ile Val Gly Leu 
            340                 345                 350         


Ser Glu Leu Pro Lys Ile Tyr Glu Leu Met Glu Gln Gly Lys Ile Leu 
        355                 360                 365             


Gly Arg Tyr Val Val Asp Thr Ser Lys 
    370                 375         


<210>  5
<211>  1053
<212>  DNA
<213>  Issatchenkia orientalis


<220>
<221>  CDS
<222>  (1)..(1053)

<400>  5
atg tct tac gaa atc cca caa aca caa aag gcc tgt gtc ttt tac gaa         48
Met Ser Tyr Glu Ile Pro Gln Thr Gln Lys Ala Cys Val Phe Tyr Glu           
1               5                   10                  15                

aac ggc ggc cca atc aca tac aag gac att cca gtt cca aag cca aaa         96
Asn Gly Gly Pro Ile Thr Tyr Lys Asp Ile Pro Val Pro Lys Pro Lys           
            20                  25                  30                    

cct act gag att tta gtc aag gtt ctg tac tct ggt gtc tgc cac acc        144
Pro Thr Glu Ile Leu Val Lys Val Leu Tyr Ser Gly Val Cys His Thr           
        35                  40                  45                        

gac ttg cac gca tgg aag ggt gac tgg cct cta gct acc aag ttg cca        192
Asp Leu His Ala Trp Lys Gly Asp Trp Pro Leu Ala Thr Lys Leu Pro           
    50                  55                  60                            

ttg gtt ggt ggt cac gaa ggt gcc ggt gtt gtt gtt gcc aag ggt gaa        240
Leu Val Gly Gly His Glu Gly Ala Gly Val Val Val Ala Lys Gly Glu           
65                  70                  75                  80            

aac gtc acc tct ttt gag att ggt gat tac gca ggt atc aag tgg ttg        288
Asn Val Thr Ser Phe Glu Ile Gly Asp Tyr Ala Gly Ile Lys Trp Leu           
                85                  90                  95                

aat ggt tca tgt atg ggt tgt gaa ttc tgt gaa caa ggt gct gaa cca        336
Asn Gly Ser Cys Met Gly Cys Glu Phe Cys Glu Gln Gly Ala Glu Pro           
            100                 105                 110                   

aac tgt cct aag gcc gac ttg agt ggt tac acc cac gac ggt tcc ttc        384
Asn Cys Pro Lys Ala Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe           
        115                 120                 125                       

caa cag tat gct act gct gac gct att caa gct gca cac atc tcc aag        432
Gln Gln Tyr Ala Thr Ala Asp Ala Ile Gln Ala Ala His Ile Ser Lys           
    130                 135                 140                           

gaa acc gac ttg gct ggt gtt gct cca atc ttg tgt gca ggt gtc act        480
Glu Thr Asp Leu Ala Gly Val Ala Pro Ile Leu Cys Ala Gly Val Thr           
145                 150                 155                 160           

gtc tac aag gct tta aag act gca gac ctt aga gca ggt gaa tgg gtt        528
Val Tyr Lys Ala Leu Lys Thr Ala Asp Leu Arg Ala Gly Glu Trp Val           
                165                 170                 175               

tgt att tcc ggt gca gct ggt ggt tta ggt tct ctt gct att caa tat        576
Cys Ile Ser Gly Ala Ala Gly Gly Leu Gly Ser Leu Ala Ile Gln Tyr           
            180                 185                 190                   

gca aag gct atg ggt ctg aga gtt gtt ggt att gac ggt ggt gac gaa        624
Ala Lys Ala Met Gly Leu Arg Val Val Gly Ile Asp Gly Gly Asp Glu           
        195                 200                 205                       

aag aag gaa ttg tgt aaa tcc ctt ggt gct gaa gca ttt att gat ttc        672
Lys Lys Glu Leu Cys Lys Ser Leu Gly Ala Glu Ala Phe Ile Asp Phe           
    210                 215                 220                           

aca aag acc aag gat atc gtc aag gct gtc caa gag gca acc aat ggt        720
Thr Lys Thr Lys Asp Ile Val Lys Ala Val Gln Glu Ala Thr Asn Gly           
225                 230                 235                 240           

ggt cca cat ggt gtc atc aat gtc tct gtc tct gaa gct gca att tct        768
Gly Pro His Gly Val Ile Asn Val Ser Val Ser Glu Ala Ala Ile Ser           
                245                 250                 255               

caa tct tgt gaa tac gtt aga cct cta ggt aag gtt gtt ctt gtt ggt        816
Gln Ser Cys Glu Tyr Val Arg Pro Leu Gly Lys Val Val Leu Val Gly           
            260                 265                 270                   

tta cca gca ggc gca caa gtc aaa act ggt gtc ttt gaa gcc gtt gtc        864
Leu Pro Ala Gly Ala Gln Val Lys Thr Gly Val Phe Glu Ala Val Val           
        275                 280                 285                       

aag tct att gaa att aag ggt tct tat gtc ggt aac aga aag gat acc        912
Lys Ser Ile Glu Ile Lys Gly Ser Tyr Val Gly Asn Arg Lys Asp Thr           
    290                 295                 300                           

gcc gaa gca ctt gac ttc tac act aga ggc ttg gtc aag tct cca ttc        960
Ala Glu Ala Leu Asp Phe Tyr Thr Arg Gly Leu Val Lys Ser Pro Phe           
305                 310                 315                 320           

aag att gtc ggt tta tcc gaa ttg cca aaa gtc ttt gaa ctc atg gaa       1008
Lys Ile Val Gly Leu Ser Glu Leu Pro Lys Val Phe Glu Leu Met Glu           
                325                 330                 335               

cag ggt aag att tta ggt aga atg gtc tta gac acc tcc aaa taa           1053
Gln Gly Lys Ile Leu Gly Arg Met Val Leu Asp Thr Ser Lys                   
            340                 345                 350                   


<210>  6
<211>  350
<212>  PRT
<213>  Issatchenkia orientalis

<400>  6

Met Ser Tyr Glu Ile Pro Gln Thr Gln Lys Ala Cys Val Phe Tyr Glu 
1               5                   10                  15      


Asn Gly Gly Pro Ile Thr Tyr Lys Asp Ile Pro Val Pro Lys Pro Lys 
            20                  25                  30          


Pro Thr Glu Ile Leu Val Lys Val Leu Tyr Ser Gly Val Cys His Thr 
        35                  40                  45              


Asp Leu His Ala Trp Lys Gly Asp Trp Pro Leu Ala Thr Lys Leu Pro 
    50                  55                  60                  


Leu Val Gly Gly His Glu Gly Ala Gly Val Val Val Ala Lys Gly Glu 
65                  70                  75                  80  


Asn Val Thr Ser Phe Glu Ile Gly Asp Tyr Ala Gly Ile Lys Trp Leu 
                85                  90                  95      


Asn Gly Ser Cys Met Gly Cys Glu Phe Cys Glu Gln Gly Ala Glu Pro 
            100                 105                 110         


Asn Cys Pro Lys Ala Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe 
        115                 120                 125             


Gln Gln Tyr Ala Thr Ala Asp Ala Ile Gln Ala Ala His Ile Ser Lys 
    130                 135                 140                 


Glu Thr Asp Leu Ala Gly Val Ala Pro Ile Leu Cys Ala Gly Val Thr 
145                 150                 155                 160 


Val Tyr Lys Ala Leu Lys Thr Ala Asp Leu Arg Ala Gly Glu Trp Val 
                165                 170                 175     


Cys Ile Ser Gly Ala Ala Gly Gly Leu Gly Ser Leu Ala Ile Gln Tyr 
            180                 185                 190         


Ala Lys Ala Met Gly Leu Arg Val Val Gly Ile Asp Gly Gly Asp Glu 
        195                 200                 205             


Lys Lys Glu Leu Cys Lys Ser Leu Gly Ala Glu Ala Phe Ile Asp Phe 
    210                 215                 220                 


Thr Lys Thr Lys Asp Ile Val Lys Ala Val Gln Glu Ala Thr Asn Gly 
225                 230                 235                 240 


Gly Pro His Gly Val Ile Asn Val Ser Val Ser Glu Ala Ala Ile Ser 
                245                 250                 255     


Gln Ser Cys Glu Tyr Val Arg Pro Leu Gly Lys Val Val Leu Val Gly 
            260                 265                 270         


Leu Pro Ala Gly Ala Gln Val Lys Thr Gly Val Phe Glu Ala Val Val 
        275                 280                 285             


Lys Ser Ile Glu Ile Lys Gly Ser Tyr Val Gly Asn Arg Lys Asp Thr 
    290                 295                 300                 


Ala Glu Ala Leu Asp Phe Tyr Thr Arg Gly Leu Val Lys Ser Pro Phe 
305                 310                 315                 320 


Lys Ile Val Gly Leu Ser Glu Leu Pro Lys Val Phe Glu Leu Met Glu 
                325                 330                 335     


Gln Gly Lys Ile Leu Gly Arg Met Val Leu Asp Thr Ser Lys 
            340                 345                 350 


<210>  7
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  7
cttgtgaata cgttagacct cta                                               23


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  8
gagacttgac caagcctcta                                                   20


<210>  9
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  9
catgtgacta tgttagagca act                                               23


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  10
ggagatctga ccttaccttc a                                                 21


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  11
cccagtatgt cagaaccttg                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  12
gtgacttcac taaacccctg                                                   20


<210>  13
<211>  348
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  13

Met Ser Ile Pro Glu Thr Gln Lys Gly Val Ile Phe Tyr Glu Ser His 
1               5                   10                  15      


Gly Lys Leu Glu Tyr Lys Asp Ile Pro Val Pro Lys Pro Lys Ala Asn 
            20                  25                  30          


Glu Leu Leu Ile Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu 
        35                  40                  45              


His Ala Trp His Gly Asp Trp Pro Leu Pro Val Lys Leu Pro Leu Val 
    50                  55                  60                  


Gly Gly His Glu Gly Ala Gly Val Val Val Gly Met Gly Glu Asn Val 
65                  70                  75                  80  


Lys Gly Trp Lys Ile Gly Asp Tyr Ala Gly Ile Lys Trp Leu Asn Gly 
                85                  90                  95      


Ser Cys Met Ala Cys Glu Tyr Cys Glu Leu Gly Asn Glu Ser Asn Cys 
            100                 105                 110         


Pro His Ala Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Gln 
        115                 120                 125             


Tyr Ala Thr Ala Asp Ala Val Gln Ala Ala His Ile Pro Gln Gly Thr 
    130                 135                 140                 


Asp Leu Ala Gln Val Ala Pro Ile Leu Cys Ala Gly Ile Thr Val Tyr 
145                 150                 155                 160 


Lys Ala Leu Lys Ser Ala Asn Leu Met Ala Gly His Trp Val Ala Ile 
                165                 170                 175     


Ser Gly Ala Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys 
            180                 185                 190         


Ala Met Gly Tyr Arg Val Leu Gly Ile Asp Gly Gly Glu Gly Lys Glu 
        195                 200                 205             


Glu Leu Phe Arg Ser Ile Gly Gly Glu Val Phe Ile Asp Phe Thr Lys 
    210                 215                 220                 


Glu Lys Asp Ile Val Gly Ala Val Leu Lys Ala Thr Asp Gly Gly Ala 
225                 230                 235                 240 


His Gly Val Ile Asn Val Ser Val Ser Glu Ala Ala Ile Glu Ala Ser 
                245                 250                 255     


Thr Arg Tyr Val Arg Ala Asn Gly Thr Thr Val Leu Val Gly Met Pro 
            260                 265                 270         


Ala Gly Ala Lys Cys Cys Ser Asp Val Phe Asn Gln Val Val Lys Ser 
        275                 280                 285             


Ile Ser Ile Val Gly Ser Tyr Val Gly Asn Arg Ala Asp Thr Arg Glu 
    290                 295                 300                 


Ala Leu Asp Phe Phe Ala Arg Gly Leu Val Lys Ser Pro Ile Lys Val 
305                 310                 315                 320 


Val Gly Leu Ser Thr Leu Pro Glu Ile Tyr Glu Lys Met Glu Lys Gly 
                325                 330                 335     


Gln Ile Val Gly Arg Tyr Val Val Asp Thr Ser Lys 
            340                 345             


<210>  14
<211>  348
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  14

Met Ser Ile Pro Glu Thr Gln Lys Ala Ile Ile Phe Tyr Glu Ser Asn 
1               5                   10                  15      


Gly Lys Leu Glu His Lys Asp Ile Pro Val Pro Lys Pro Lys Pro Asn 
            20                  25                  30          


Glu Leu Leu Ile Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu 
        35                  40                  45              


His Ala Trp His Gly Asp Trp Pro Leu Pro Thr Lys Leu Pro Leu Val 
    50                  55                  60                  


Gly Gly His Glu Gly Ala Gly Val Val Val Gly Met Gly Glu Asn Val 
65                  70                  75                  80  


Lys Gly Trp Lys Ile Gly Asp Tyr Ala Gly Ile Lys Trp Leu Asn Gly 
                85                  90                  95      


Ser Cys Met Ala Cys Glu Tyr Cys Glu Leu Gly Asn Glu Ser Asn Cys 
            100                 105                 110         


Pro His Ala Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Glu 
        115                 120                 125             


Tyr Ala Thr Ala Asp Ala Val Gln Ala Ala His Ile Pro Gln Gly Thr 
    130                 135                 140                 


Asp Leu Ala Glu Val Ala Pro Ile Leu Cys Ala Gly Ile Thr Val Tyr 
145                 150                 155                 160 


Lys Ala Leu Lys Ser Ala Asn Leu Arg Ala Gly His Trp Ala Ala Ile 
                165                 170                 175     


Ser Gly Ala Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys 
            180                 185                 190         


Ala Met Gly Tyr Arg Val Leu Gly Ile Asp Gly Gly Pro Gly Lys Glu 
        195                 200                 205             


Glu Leu Phe Thr Ser Leu Gly Gly Glu Val Phe Ile Asp Phe Thr Lys 
    210                 215                 220                 


Glu Lys Asp Ile Val Ser Ala Val Val Lys Ala Thr Asn Gly Gly Ala 
225                 230                 235                 240 


His Gly Ile Ile Asn Val Ser Val Ser Glu Ala Ala Ile Glu Ala Ser 
                245                 250                 255     


Thr Arg Tyr Cys Arg Ala Asn Gly Thr Val Val Leu Val Gly Leu Pro 
            260                 265                 270         


Ala Gly Ala Lys Cys Ser Ser Asp Val Phe Asn His Val Val Lys Ser 
        275                 280                 285             


Ile Ser Ile Val Gly Ser Tyr Val Gly Asn Arg Ala Asp Thr Arg Glu 
    290                 295                 300                 


Ala Leu Asp Phe Phe Ala Arg Gly Leu Val Lys Ser Pro Ile Lys Val 
305                 310                 315                 320 


Val Gly Leu Ser Ser Leu Pro Glu Ile Tyr Glu Lys Met Glu Lys Gly 
                325                 330                 335     


Gln Ile Ala Gly Arg Tyr Val Val Asp Thr Ser Lys 
            340                 345             


<210>  15
<211>  375
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  15

Met Leu Arg Thr Ser Thr Leu Phe Thr Arg Arg Val Gln Pro Ser Leu 
1               5                   10                  15      


Phe Ser Arg Asn Ile Leu Arg Leu Gln Ser Thr Ala Ala Ile Pro Lys 
            20                  25                  30          


Thr Gln Lys Gly Val Ile Phe Tyr Glu Asn Lys Gly Lys Leu His Tyr 
        35                  40                  45              


Lys Asp Ile Pro Val Pro Glu Pro Lys Pro Asn Glu Ile Leu Ile Asn 
    50                  55                  60                  


Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu His Ala Trp His Gly 
65                  70                  75                  80  


Asp Trp Pro Leu Pro Val Lys Leu Pro Leu Val Gly Gly His Glu Gly 
                85                  90                  95      


Ala Gly Val Val Val Lys Leu Gly Ser Asn Val Lys Gly Trp Lys Val 
            100                 105                 110         


Gly Asp Leu Ala Gly Ile Lys Trp Leu Asn Gly Ser Cys Met Thr Cys 
        115                 120                 125             


Glu Phe Cys Glu Ser Gly His Glu Ser Asn Cys Pro Asp Ala Asp Leu 
    130                 135                 140                 


Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Gln Phe Ala Thr Ala Asp 
145                 150                 155                 160 


Ala Ile Gln Ala Ala Lys Ile Gln Gln Gly Thr Asp Leu Ala Glu Val 
                165                 170                 175     


Ala Pro Ile Leu Cys Ala Gly Val Thr Val Tyr Lys Ala Leu Lys Glu 
            180                 185                 190         


Ala Asp Leu Lys Ala Gly Asp Trp Val Ala Ile Ser Gly Ala Ala Gly 
        195                 200                 205             


Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Thr Ala Met Gly Tyr Arg 
    210                 215                 220                 


Val Leu Gly Ile Asp Ala Gly Glu Glu Lys Glu Lys Leu Phe Lys Lys 
225                 230                 235                 240 


Leu Gly Gly Glu Val Phe Ile Asp Phe Thr Lys Thr Lys Asn Met Val 
                245                 250                 255     


Ser Asp Ile Gln Glu Ala Thr Lys Gly Gly Pro His Gly Val Ile Asn 
            260                 265                 270         


Val Ser Val Ser Glu Ala Ala Ile Ser Leu Ser Thr Glu Tyr Val Arg 
        275                 280                 285             


Pro Cys Gly Thr Val Val Leu Val Gly Leu Pro Ala Asn Ala Tyr Val 
    290                 295                 300                 


Lys Ser Glu Val Phe Ser His Val Val Lys Ser Ile Asn Ile Lys Gly 
305                 310                 315                 320 


Ser Tyr Val Gly Asn Arg Ala Asp Thr Arg Glu Ala Leu Asp Phe Phe 
                325                 330                 335     


Ser Arg Gly Leu Ile Lys Ser Pro Ile Lys Ile Val Gly Leu Ser Glu 
            340                 345                 350         


Leu Pro Lys Val Tyr Asp Leu Met Glu Lys Gly Lys Ile Leu Gly Arg 
        355                 360                 365             


Tyr Val Val Asp Thr Ser Lys 
    370                 375 


