
                                SEQUENCE LISTING

<110> Bigner, Darell D.
      Kuan, Chien-Tsun
      Pastan, Ira H.
      Piao, Hailan

<120> ANTIBODIES FOR TUMOR GANGLIOSIDES
  

<130> 000250.00151

<150> 61/419317           
<151> 2010-12-03  

<150> 61/416096           
<151> 2010-11-22  

<160> 68

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 15
<212> PRT
<213> Artificical Sequence

<220> 
<223> hybrid human and mouse

<400> 1
Asn Pro Met Asp Ser Arg Thr Asn Tyr Asn Lys Lys Phe Lys Ser
 1               5                  10                  15

<210> 2
<211> 15
<212> PRT
<213> Artificical Sequence

<220> 
<223> hybrid human and mouse

<400> 2
Leu Tyr Gly Glu Ser Val Glu Ser Tyr Gly Asn Asn Phe Met His
 1               5                  10                  15

<210> 3
<211> 45
<212> DNA
<213> Artificical Sequence

<220> 
<223> hybrid human and mouse

<400> 3
aatccaatgg actcccgtac taactataat aagaagttca agagc                      45

<210> 4
<211> 45
<212> DNA
<213> Artificical Sequence

<220> 
<223> hybrid human and mouse

<400> 4
ttgtacgggg aaagtgttga gagttatggc aataatttta tgcac                      45

<210> 5
<211> 745
<212> DNA
<213> Artificical Sequence

<220> 
<223> hybrid human and mouse; DMab14-86184

<400> 5
catatgcagg tccaactgca gcagcctggg gctgaactgg tgaagcctgg ggcttcagtg      60
aagctgtcct gcaaggcttc tggctacacc ttcaccaggt actggatgca ctgggtgaga     120
cagaggcctg gacaaggtct tgagtggatt ggagagatta atccaatgga ctcccgtact     180
aactataata agaagttcaa gagcaaggcc acactgactg tagacaaatc ctccagcaca     240
gcctacatgc aactcagcag cctgaaatct gaggactctg cggtctatta ctgtgcaaga     300
ccaggtcggg ctaggggtat ggactactgg ggtcaaggaa actcagtcac cgtctcctca     360
ggcggagggg gatctggtgg tggcggatct ggaggtggcg gcagcggtaa cattgtgctg     420
acccaatctc cagcttcttt ggctgtgtct ctagggcaga gggccatcat atcctgcttg     480
tacggggaaa gtgttgagag ttatggcaat aattttatgc actggtacca gcagaaacca     540
ggacaggcac ccaaactcct catctatctt gcatccaacc tagaatctgg ggtccctgcc     600
aggttcagtg gcagtgggtc taggacagac ttcaccctca ccattgatcc tgtggaggct     660
gatgatgctg caacctatta ctgtcagcaa aataatgagg atcccacgtt cggagggggg     720
accaagctgg aaataaaaaa agctt                                           745

<210> 6
<211> 248
<212> PRT
<213> Artificical Sequence

<220> 
<223> hybrid human and mouse; DMab14-86184

<400> 6
His Met Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro
 1               5                  10                  15
Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr
            20                  25                  30
Arg Tyr Trp Met His Trp Val Arg Gln Arg Pro Gly Gln Gly Leu Glu
        35                  40                  45
Trp Ile Gly Glu Ile Asn Pro Met Asp Ser Arg Thr Asn Tyr Asn Lys
    50                  55                  60
Lys Phe Lys Ser Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr
65                  70                  75                  80
Ala Tyr Met Gln Leu Ser Ser Leu Lys Ser Glu Asp Ser Ala Val Tyr
                85                  90                  95
Tyr Cys Ala Arg Pro Gly Arg Ala Arg Gly Met Asp Tyr Trp Gly Gln
            100                 105                 110
Gly Asn Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly
        115                 120                 125
Gly Ser Gly Gly Gly Gly Ser Gly Asn Ile Val Leu Thr Gln Ser Pro
    130                 135                 140
Ala Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Ile Ile Ser Cys Leu
145                 150                 155                 160
Tyr Gly Glu Ser Val Glu Ser Tyr Gly Asn Asn Phe Met His Trp Tyr
                165                 170                 175
Gln Gln Lys Pro Gly Gln Ala Pro Lys Leu Leu Ile Tyr Leu Ala Ser
            180                 185                 190
Asn Leu Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Arg
        195                 200                 205
Thr Asp Phe Thr Leu Thr Ile Asp Pro Val Glu Ala Asp Asp Ala Ala
    210                 215                 220
Thr Tyr Tyr Cys Gln Gln Asn Asn Glu Asp Pro Thr Phe Gly Gly Gly
225                 230                 235                 240
Thr Lys Leu Glu Ile Lys Lys Ala
                245

<210> 7
<211> 15
<212> PRT
<213> Artificical Sequence

<220> 
<223> hybrid human and mouse

<400> 7
Asn Pro Ser Asn Gly Arg Thr Asn Tyr Asn Glu Lys Phe Lys Ser
 1               5                  10                  15

<210> 8
<211> 15
<212> PRT
<213> Artificical Sequence

<220> 
<223> hybrid human and mouse

<400> 8
Arg Ala Ser Glu Ser Val Glu Ser Tyr Gly Asn Asn Phe Met His
 1               5                  10                  15

<210> 9
<211> 45
<212> DNA
<213> Artificical Sequence

<220> 
<223> hybrid human and mouse

<400> 9
aatcctagca acggtcgtac taactataat gagaagttca agagc                      45

<210> 10
<211> 45
<212> DNA
<213> Artificical Sequence

<220> 
<223> hybrid human and mouse

<400> 10
agagccagtg aaagtgttga gagttatggc aataatttta tgcac                      45

<210> 11
<211> 115
<212> PRT
<213> Artificical Sequence

<220> 
<223> ScFv antibodies or portion thereof; GMab1-VH

<400> 11
Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala
 1               5                  10                  15
Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr
            20                  25                  30
Tyr Met Asn Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile
        35                  40                  45
Gly Asp Ile Asn Pro Asn Asn Gly Gly Thr Ser Tyr Asn Gln Lys Phe
    50                  55                  60
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr
65                  70                  75                  80
Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys
                85                  90                  95
Ala Thr Tyr Gly Leu Gly Gly Tyr Trp Gly Gln Gly Thr Thr Leu Thr
            100                 105                 110
Val Ser Ser
        115

<210> 12
<211> 108
<212> PRT
<213> Artificical Sequence

<220> 
<223> ScFv antibodies or portion thereof; GMab1-VL

<400> 12
Gly Asn Ile Val Met Thr Gln Ser Pro Lys Ser Met Ser Met Ser Val
 1               5                  10                  15
Gly Glu Arg Val Thr Gln Thr Cys Lys Ala Ser Glu Asn Val Val Thr
            20                  25                  30
Tyr Val Ser Trp Tyr Gln Leu Lys Pro Glu Gln Ser Pro Lys Leu Leu
        35                  40                  45
Ile Tyr Gly Ala Ser Asn Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr
    50                  55                  60
Gly Ser Gly Ser Ala Thr Asp Phe Thr Leu Thr Ile Ser Ser Val Gln
65                  70                  75                  80
Ala Glu Asp Leu Ala Asp Tyr His Cys Gly Gln Gly Tyr Ser Tyr Pro
                85                  90                  95
Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys
            100                 105

<210> 13
<211> 115
<212> PRT
<213> Artificical Sequence

<220> 
<223> ScFv antibodies or portion thereof; GMab2-VH

<400> 13
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly
 1               5                  10                  15
Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
            20                  25                  30
Ala Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val
        35                  40                  45
Ala Thr Ile Ser Asp Gly Gly Tyr Tyr Thr Tyr Tyr Pro Asp Asn Val
    50                  55                  60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Asn Leu Tyr
65                  70                  75                  80
Leu Gln Met Ser His Leu Lys Ser Gly Asp Thr Ala Met Tyr Tyr Cys
                85                  90                  95
Ala Arg Tyr Asp Ser Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr
            100                 105                 110
Val Ser Ser
        115

<210> 14
<211> 111
<212> PRT
<213> Artificical Sequence

<220> 
<223> ScFv antibodies or portion thereof; GMab2-VL

<400> 14
Gly Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu
 1               5                  10                  15
Gly Gln Arg Ala Thr Ile Ser Tyr Arg Ala Ser Lys Ser Val Ser Thr
            20                  25                  30
Ser Gly Tyr Ser Tyr Met His Trp Asn Gln Gln Lys Pro Gly Gln Pro
        35                  40                  45
Pro Arg Leu Leu Ile Tyr Leu Val Ser Asn Leu Glu Ser Gly Val Pro
    50                  55                  60
Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile
65                  70                  75                  80
His Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln His Ile
                85                  90                  95
Arg Glu Leu Thr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys
            100                 105                 110

<210> 15
<211> 727
<212> DNA
<213> Artificical Sequence

<220> 
<223> ScFv antibodies or portion thereof; DMab1-scFv

<400> 15
catatggagg tccagctgca acaatctgga cctgagctgg tgaagcctgg ggcttcagtg      60
aagatatcct gtaaggcttc tggatacacg ttcactgact actacatgaa ctgggtgaag     120
cagagccatg gaaagagcct tgagtggatt ggagatatta atcctaacaa tggtggtact     180
agctacaacc agaagttcaa gggcaaggcc acattgactg tagacaagtc ctccagcacc     240
gcctacatgg agctccgcag cctgacctct gaggactctg cagtctatta ctgtgcaact     300
tatggactgg gaggctactg gggccaaggc accactctca cagtctcctc aggcggaggg     360
ggatccggtg gtggcggatc tggaggtggc ggcagcggga acattgtaat gacccaatct     420
cccaaatcca tgtccatgtc agtaggagag agggtcacct tgacctgcaa ggccagtgag     480
aatgtggtta cttatgtttc ctggtatcaa ctgaaaccag agcagtctcc taaactgctg     540
atatacgggg catccaaccg gtacactggg gtccccgatc gcttcacagg cagtggatct     600
gcaacagatt tcactctgac catcagcagt gtgcaggctg aagaccttgc agattatcac     660
tgtggacagg gttacagcta tccgtacacg ttcggagggg ggaccaagct ggaaataaaa     720
aaagctt                                                               727

<210> 16
<211> 723
<212> DNA
<213> Artificical Sequence

<220> 
<223> ScFv antibodies or portion thereof; DMab2-scFv

<400> 16
gaagtgcagc tggtggagtc tgggggaggc ttagtgaagc ctggagggtc cctgaaactc      60
tcctgtgcag cctctggatt cactttcagt agctatgcca tgtcttgggt tcgccagact     120
ccggaaaaga ggctggagtg ggtcgcaacc attagtgatg gtggttatta cacgtactat     180
ccagacaatg taaagggccg attcaccatc tccagagaca atgccaagaa caacctgtac     240
ctgcaaatga gccatctgaa gtctggggac acagccatgt attactgtgc aagatatgac     300
tcctttgact actggggcca aggcaccact ctcacagtct cctcaggcgg agggggatcc     360
ggtggtggcg gatctggagg tggcggcagc ggtgacattg tgctgacaca gtctcctgct     420
tccttagctg tatctctggg gcagagggcc accatctcat acagggccag caaaagtgtc     480
agtacatctg gctatagtta tatgcactgg aaccaacaga aaccaggaca gccacccaga     540
ctcctcatct atcttgtatc caacctagaa tctggggtcc ctgccaggtt cagtggcagt     600
gggtctggga cagacttcac cctcaacatc catcctgtgg aggaggagga tgctgcaacc     660
tattactgtc agcacattag ggagcttaca acgttcggag gggggaccaa gctggaaata     720
aaa                                                                   723

<210> 17
<211> 722
<212> DNA
<213> Artificical Sequence

<220> 
<223> scFv; GMab1

<400> 17
atggaggtcc agctgcaaca atctggacct gagctggtga agcctggggc ttcagtgaag      60
atatcctgta aggcttctgg atacacgttc actgactact acatgaactg ggtgaagcag     120
agccatggaa agagccttga gtggattgga gatattaatc ctaacaatgg tggtactagc     180
tacaaccaga agttcaaggg caaggccaca ttgactgtag acaagtcctc cagcaccgcc     240
tacatggagc tccgcagcct gacctctgag gactctgcag tctattactg tgcaacttat     300
ggcctgggag gctactgggg ccaaggcacc actctcacag tctcctcagg cggaggggga     360
tctggtggtg gcggatctgg aggtggcggc agcgggaata ttgtaatgac ccaatctccc     420
aaatccatgt ccatgtcagt aggagagagg gtcaccttga cctgcaaggc cagtgagaat     480
gtggttactt atgtttcctg gtatcaacag aaaccagagc agtctcctaa actgctgata     540
tacggggcat ccaaccggta cactggggtc cccgatcgct tcacaggcag tggttctgca     600
acagatttca ctctgaccat cagcagtgtg caggctgaag accttgcaga ttatcactgt     660
ggacagggtt acagctatcc gtacacgttc ggagggggga ccaagctgga agtaaaaaaa     720
gt                                                                    722

<210> 18
<211> 736
<212> DNA
<213> Artificical Sequence

<220> 
<223> scFv; GMab2

<400> 18
catatggaag tgcagctggt ggagtctggg ggaggcttag tgaagcctgg agggtccctg      60
aaactctcct gtgcagcctc tggattcact ttcagtagct atgccatgtc ttgggctcgc     120
cagactccgg aaaagaggct ggagtgggtc gcaaccatta gtgatggtgg ttattacacg     180
tactatccag acaatgtaaa gggccgattc accatctcca gagacaatgc caagaacaac     240
ctgtacctgc aaatgagcca tctgaagtct ggggacacag ccatgtatta ctgtgcaaga     300
tatgactcct ttgactactg gggccaaggc accactctca cagtctcctc aggcggaggg     360
ggatccggtg gtggcggatc tagaggtggc ggcagcggtg acattgtgct gacacagtct     420
cctgcttcct tagctgtatc tctggggcag agggccacca tctcatacag ggccagcaaa     480
agtgtcagta catctggcta tagttatatg cactggaacc aacagaaacc aggacagcca     540
cccagactcc tcatctatct tgtatccaac ctagaatctg gggtccctgc caggttcagt     600
ggcagtgggt ctgggacaga cttcaccctc aacatccatc ctgtggagga ggaggatgct     660
gcaacctatt actgtcagca cattagggag cttacaacgt tcggaggggg gaccaagctg     720
gaaataaaaa aagctt                                                     736

<210> 19
<211> 732
<212> DNA
<213> Artificical Sequence

<220> 
<223> DMab14 scFV

<400> 19
caggtccaac tgcagcagcc tggggctgaa ctggtgaagc ctggggcttc agtgaagctg      60
tcctgcaagg cttctggcta caccttcacc aggtactgga tgcactgggt gagacagagg     120
cctggacaag gtcttgagtg gattggagag attaatccta gcaacggtcg tactaactat     180
aatgagaagt tcaagagcaa ggccacactg actgtagaca aatcctccag cacagcctac     240
atgcaactca gcagcctgaa atctgaggac tctgcggtct attactgtgc aagaccaggt     300
cgggctaggg gtatggacta ctggggtcaa ggaaactcag tcaccgtctc ctcaggcgga     360
gggggatccg gtggtggcgg atctggaggt ggcggcagcg gtaacattgt gctgacccaa     420
tctccagctt ctttggctgt gtctctaggg cagagggcca ccatatcctg cagagccagt     480
gaaagtgttg agagttatgg caataatttt atgcactggt accagcagaa accaggacag     540
gcacccaaac tcctcatcta tcttgcatcc aacctagaat ctggggtccc tgccaggttc     600
agtggcagtg ggtctaggac agacttcacc ctcaccatcg atcccgtgga ggctgatgat     660
gctgcaacct attactgtca gcaaaataat gaggatccca cgttcggagg ggggaccaag     720
ctggaaataa aa                                                         732

<210> 20
<211> 225
<212> PRT
<213> Artificical Sequence

<220> 
<223> scFv GMab1

<400> 20
Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala
 1               5                  10                  15
Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr
            20                  25                  30
Tyr Met Asn Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile
        35                  40                  45
Gly Asp Ile Asn Pro Asn Asn Gly Gly Thr Ser Tyr Asn Gln Lys Phe
    50                  55                  60
Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr
65                  70                  75                  80
Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys
                85                  90                  95
Ala Thr Tyr Gly Leu Gly Gly Tyr Trp Gly Gln Gly Thr Thr Leu Thr
            100                 105                 110
Val Ser Ser Gly Asn Ile Val Met Thr Gln Ser Pro Lys Ser Met Ser
        115                 120                 125
Met Ser Val Gly Glu Arg Val Thr Leu Thr Cys Lys Ala Ser Glu Asn
    130                 135                 140
Val Val Thr Tyr Val Ser Trp Tyr Gln Gln Lys Pro Glu Gln Ser Pro
145                 150                 155                 160
Lys Leu Leu Ile Tyr Gly Ala Ser Asn Arg Tyr Thr Gly Val Pro Asp
                165                 170                 175
Arg Phe Thr Gly Ser Gly Ser Ala Thr Asp Phe Thr Leu Thr Ile Ser
            180                 185                 190
Ser Val Gln Ala Glu Asp Leu Ala Asp Tyr His Cys Gly Gln Gly Tyr
        195                 200                 205
Ser Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Val Lys Lys
    210                 215                 220
Ala
225

<210> 21
<211> 244
<212> PRT
<213> Artificical Sequence

<220> 
<223> scFv; GMab2

<400> 21
Met Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly
 1               5                  10                  15
Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser
            20                  25                  30
Tyr Ala Met Ser Trp Ala Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp
        35                  40                  45
Val Ala Thr Ile Ser Asp Gly Gly Tyr Tyr Thr Tyr Tyr Pro Asp Asn
    50                  55                  60
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Asn Leu
65                  70                  75                  80
Tyr Leu Gln Met Ser His Leu Lys Ser Gly Asp Thr Ala Met Tyr Tyr
                85                  90                  95
Cys Ala Arg Tyr Asp Ser Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu
            100                 105                 110
Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Arg Gly
        115                 120                 125
Gly Gly Ser Gly Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala
    130                 135                 140
Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Tyr Arg Ala Ser Lys Ser
145                 150                 155                 160
Val Ser Thr Ser Gly Tyr Ser Tyr Met His Trp Asn Gln Gln Lys Pro
                165                 170                 175
Gly Gln Pro Pro Arg Leu Leu Ile Tyr Leu Val Ser Asn Leu Glu Ser
            180                 185                 190
Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr
        195                 200                 205
Leu Asn Ile His Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys
    210                 215                 220
Gln His Ile Arg Glu Leu Thr Thr Phe Gly Gly Gly Thr Lys Leu Glu
225                 230                 235                 240
Ile Lys Lys Ala


<210> 22
<211> 230
<212> PRT
<213> Artificical Sequence

<220> 
<223> scFv; DMab14

<400> 22
Met Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly
 1               5                  10                  15
Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg
            20                  25                  30
Tyr Trp Met His Trp Val Arg Gln Arg Pro Gly Gln Gly Leu Glu Trp
        35                  40                  45
Ile Gly Glu Ile Asn Pro Ser Asn Gly Arg Thr Asn Tyr Asn Glu Lys
    50                  55                  60
Phe Lys Ser Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala
65                  70                  75                  80
Tyr Met Gln Leu Ser Ser Leu Lys Ser Glu Asp Ser Ala Val Tyr Tyr
                85                  90                  95
Cys Ala Arg Pro Gly Arg Ala Arg Gly Met Asp Tyr Trp Gly Gln Gly
            100                 105                 110
Asn Ser Val Thr Val Ser Ser Gly Asn Ile Val Leu Thr Gln Ser Pro
        115                 120                 125
Ala Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg
    130                 135                 140
Ala Ser Glu Ser Val Glu Ser Tyr Gly Asn Asn Phe Met His Trp Tyr
145                 150                 155                 160
Gln Gln Lys Pro Gly Gln Ala Pro Lys Leu Leu Ile Tyr Leu Ala Ser
                165                 170                 175
Asn Leu Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Arg
            180                 185                 190
Thr Asp Phe Thr Leu Thr Ile Asp Pro Val Glu Ala Asp Asp Ala Ala
        195                 200                 205
Thr Tyr Tyr Cys Gln Gln Asn Asn Glu Asp Pro Thr Phe Gly Gly Gly
    210                 215                 220
Thr Lys Leu Glu Ile Lys
225                 230

<210> 23
<211> 36
<212> DNA
<213> Artificical Sequence

<220> 
<223> primer

<400> 23
ggccagtgga tagtcagatg ggggtgtcgt tttggc                                36

<210> 24
<211> 21
<212> DNA
<213> Artificical Sequence

<220> 
<223> primer

<400> 24
ggatacagtt ggtgcagcat c                                                21

<210> 25
<211> 27
<212> DNA
<213> Artificical Sequence

<220> 
<223> primer

<400> 25
gccgccacca tggaggtcca actgcag                                          27

<210> 26
<211> 45
<212> DNA
<213> Artificical Sequence

<220> 
<223> primer

<400> 26
agatccgcca ccaccggatc cccctccgcc tgaggagacg gtgac                      45

<210> 27
<211> 45
<212> DNA
<213> Artificical Sequence

<220> 
<223> primer

<400> 27
ggtggtggcg gatctggagg tggcggcagc ggtaacattg tgctg                      45

<210> 28
<211> 30
<212> DNA
<213> Artificical Sequence

<220> 
<223> primer

<400> 28
gcagccgaat tcatttttat ttccagcttg                                       30

<210> 29
<211> 27
<212> DNA
<213> Artificical Sequence

<220> 
<223> primer

<400> 29
gccgccacca tggaggtcca actgcag                                          27

<210> 30
<211> 31
<212> DNA
<213> Artificical Sequence

<220> 
<223> primer

<400> 30
atgatgtgcg gccgctttta tttccagctt g                                     31

<210> 31
<211> 36
<212> DNA
<213> Artificical Sequence

<220> 
<221> misc_feature   
<222> (1)...(36)
<223> n = A,T,C or G

<223> primer

<400> 31
gttagtacga ccgttsnnsn nsnnaatctc tccaat                                36

<210> 32
<211> 39
<212> DNA
<213> Artificical Sequence

<220> 
<221> misc_feature   
<222> (1)...(39)
<223> n = A,T,C or G

<223> primer

<400> 32
aatatagtta gtacgsnnsn nsnnaggatt aatctctcc                             39

<210> 33
<211> 33
<212> DNA
<213> Artificical Sequence

<220> 
<221> misc_feature   
<222> (1)...(33)
<223> n = A,T,C or G

<223> primer

<400> 33
tacagtcagt gtggcsnnsn nsnngaactt ctc                                   33

<210> 34
<211> 27
<212> DNA
<213> Artificical Sequence

<220> 
<223> primer

<400> 34
gccgccacca tggaggtcca actgcag                                          27

<210> 35
<211> 31
<212> DNA
<213> Artificical Sequence

<220> 
<223> primer

<400> 35
atgatgtgcg gccgctttta tttccagctt g                                     31

<210> 36
<211> 42
<212> DNA
<213> Artificical Sequence

<220> 
<221> misc_feature   
<222> (1)...(42)
<223> n = A,T,C or G

<223> primer

<400> 36
accatatcct gcnnsnnsnn sgaaagtgtt gagagttatg gc                         42

<210> 37
<211> 42
<212> DNA
<213> Artificical Sequence

<220> 
<221> misc_feature   
<222> (1)...(42)
<223> n = A,T,C or G

<223> primer

<400> 37
accatatcct gcagannsnn snnsagtgtt gagagttatg gc                         42

<210> 38
<211> 42
<212> DNA
<213> Artificical Sequence

<220> 
<221> misc_feature   
<222> (1)...(42)
<223> n = A,T,C or G

<223> primer

<400> 38
accatatcct gcagagccnn snnsnnsgtt gagagttatg gc                         42

<210> 39
<211> 42
<212> DNA
<213> Artificical Sequence

<220> 
<221> misc_feature   
<222> (1)...(42)
<223> n = A,T,C or G

<223> primer

<400> 39
gccagtgaaa gtgttnnsnn snnsggcaat aattttatgc ac                         42

<210> 40
<211> 27
<212> DNA
<213> Artificical Sequence

<220> 
<223> primer

<400> 40
gccgccacca tggaggtcca actgcag                                          27

<210> 41
<211> 31
<212> DNA
<213> Artificical Sequence

<220> 
<223> primer

<400> 41
atgatgtgcg gccgctttta tttccagctt g                                     31

<210> 42
<211> 25
<212> DNA
<213> Artificical Sequence

<220> 
<223> primer

<400> 42
ggcgcatatg catgtccaac tgcag                                            25

<210> 43
<211> 45
<212> DNA
<213> Artificical Sequence

<220> 
<223> primer

<400> 43
agatccgcca ccaccggatc cccctccgcc tgaggagacg gtgac                      45

<210> 44
<211> 45
<212> DNA
<213> Artificical Sequence

<220> 
<223> primer

<400> 44
ggtggtggcg gatctggagg tggcggcagc ggtaacattg tgctg                      45

<210> 45
<211> 29
<212> DNA
<213> Artificical Sequence

<220> 
<223> primer

<400> 45
caagctggaa ataaaaaaag cttggcagc                                        29

<210> 46
<211> 54
<212> DNA
<213> Artificical Sequence

<220> 
<223> DMab14 hotspot

<400> 46
gagattaatc ctagcaacgg tggtggtaac tataatgaga agttcaagag caag            54

<210> 47
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> DMab14 hotspot

<400> 47
Glu Ile Asn Pro Ser Asn Gly Arg Thr Asn Tyr Asn Glu Lys Phe Lys
 1               5                  10                  15
Ser Lys


<210> 48
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> DMab14 hotspot

<400> 48
agagccagtg aaagtgttga gagttatggc aataatttta tgcac                      45

<210> 49
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> DMab14 hotspot

<400> 49
Arg Ala Ser Glu Ser Val Glu Ser Tyr Gly Asn Asn Phe Met His
 1               5                  10                  15

<210> 50
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> DMab14 wild type or mutant sequence of VH CDR1 or
      VL CDR2

<400> 50
Glu Ile Asn Pro Ser Asn Gly Arg Thr Asn Tyr Asn Glu Lys Phe Lys
 1               5                  10                  15
Ser Lys


<210> 51
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 51
Glu Ile Asn Pro Met Asp Ser Arg Thr Asn Tyr Asn Glu Lys Phe Lys
 1               5                  10                  15
Ser Lys


<210> 52
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 52
Glu Ile Asn Pro Leu Pro Pro Arg Thr Asn Tyr Asn Glu Lys Phe Lys
 1               5                  10                  15
Ser Lys


<210> 53
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 53
Arg Ala Ser Glu Ser Val Glu Ser Tyr Gly Asn Asn Phe Met His
 1               5                  10                  15

<210> 54
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 54
Arg Ala Arg Glu Ala Val Glu Ser Tyr Gly Asn Asn Phe Met His
 1               5                  10                  15

<210> 55
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 55
Pro Trp Arg Glu Ser Val Glu Ser Tyr Gly Asn Asn Phe Met His
 1               5                  10                  15

<210> 56
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 56
Leu Tyr Gly Glu Ser Val Glu Ser Tyr Gly Asn Asn Phe Met His
 1               5                  10                  15

<210> 57
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 57
Glu Ile Asn Pro Met Asp Ser Arg Thr Asn Tyr Asn Glu Lys Phe Lys
 1               5                  10                  15
Ser Lys


<210> 58
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 58
Glu Ile Asn Pro Met Asp Ser Arg Thr Asn Tyr Asn Glu Lys Phe Lys
 1               5                  10                  15
Ser Lys


<210> 59
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 59
Glu Ile Asn Pro Met Asp Ser Arg Thr Asn Tyr Asn Glu Lys Phe Lys
 1               5                  10                  15
Ser Lys


<210> 60
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 60
Glu Ile Asn Pro Leu Pro Pro Arg Thr Asn Tyr Asn Glu Lys Phe Lys
 1               5                  10                  15
Ser Lys


<210> 61
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 61
Glu Ile Asn Pro Leu Pro Pro Arg Thr Asn Tyr Asn Glu Lys Phe Lys
 1               5                  10                  15
Ser Lys


<210> 62
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 62
Glu Ile Asn Pro Leu Pro Pro Arg Thr Asn Tyr Asn Glu Lys Phe Lys
 1               5                  10                  15
Ser Lys


<210> 63
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 63
Arg Ala Arg Glu Ala Val Glu Ser Tyr Gly Asn Asn Phe Met His
 1               5                  10                  15

<210> 64
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 64
Pro Trp Arg Glu Ser Val Glu Ser Tyr Gly Asn Asn Phe Met His
 1               5                  10                  15

<210> 65
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 65
Leu Tyr Gly Glu Ser Val Glu Ser Tyr Gly Asn Asn Phe Met His
 1               5                  10                  15

<210> 66
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 66
Arg Ala Arg Glu Ala Val Glu Ser Tyr Gly Asn Asn Phe Met His
 1               5                  10                  15

<210> 67
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 67
Pro Trp Arg Glu Ser Val Glu Ser Tyr Gly Asn Asn Phe Met His
 1               5                  10                  15

<210> 68
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> sequence of VH CDR1 or VL CDR2

<400> 68
Leu Tyr Gly Glu Ser Val Glu Ser Tyr Gly Asn Asn Phe Met His
 1               5                  10                  15
