                         SEQUENCE LISTING

<110>  Shah, Kavita
 
<120>  AURORA A KINASE EFFECTORS

<130>  3220-219593

<150>  61/414,169
<151>  2010-11-16

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  401
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Arg Arg Ala Pro Ala Ala Glu Arg Leu Leu Glu Leu Gly Phe Pro 
1               5                   10                  15      


Pro Arg Cys Gly Arg Gln Glu Pro Pro Phe Pro Leu Gly Val Thr Arg 
            20                  25                  30          


Gly Trp Gly Arg Trp Pro Ile Gln Lys Arg Arg Glu Gly Ala Arg Pro 
        35                  40                  45              


Val Pro Phe Ser Glu Arg Ser Gln Glu Asp Gly Arg Gly Pro Ala Ala 
    50                  55                  60                  


Arg Ser Ser Gly Thr Leu Trp Arg Ile Arg Thr Arg Leu Ser Leu Cys 
65                  70                  75                  80  


Arg Asp Pro Glu Pro Pro Pro Pro Leu Cys Leu Leu Arg Val Ser Leu 
                85                  90                  95      


Leu Cys Ala Leu Arg Ala Gly Gly Arg Gly Ser Arg Trp Gly Glu Asp 
            100                 105                 110         


Gly Ala Arg Leu Leu Leu Leu Pro Pro Ala Arg Ala Ala Gly Asn Gly 
        115                 120                 125             


Glu Ala Glu Pro Ser Gly Gly Pro Ser Tyr Ala Gly Arg Met Leu Glu 
    130                 135                 140                 


Ser Ser Gly Cys Lys Ala Leu Lys Glu Gly Val Leu Glu Lys Arg Ser 
145                 150                 155                 160 


Asp Gly Leu Leu Gln Leu Trp Lys Lys Lys Cys Cys Ile Leu Thr Glu 
                165                 170                 175     


Glu Gly Leu Leu Leu Ile Pro Pro Lys Gln Leu Gln His Gln Gln Gln 
            180                 185                 190         


Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Pro Gly Gln Gly 
        195                 200                 205             


Pro Ala Glu Pro Ser Gln Pro Ser Gly Pro Ala Val Ala Ser Leu Glu 
    210                 215                 220                 


Pro Pro Val Lys Leu Lys Glu Leu His Phe Ser Asn Met Lys Thr Val 
225                 230                 235                 240 


Asp Cys Val Glu Arg Lys Gly Lys Tyr Met Tyr Phe Thr Val Val Met 
                245                 250                 255     


Ala Glu Gly Lys Glu Ile Asp Phe Arg Cys Pro Gln Asp Gln Gly Trp 
            260                 265                 270         


Asn Ala Glu Ile Thr Leu Gln Met Val Gln Tyr Lys Asn Arg Gln Ala 
        275                 280                 285             


Ile Leu Ala Val Lys Ser Thr Arg Gln Lys Gln Gln His Leu Val Gln 
    290                 295                 300                 


Gln Gln Pro Pro Ser Gln Pro Gln Pro Gln Pro Gln Leu Gln Pro Gln 
305                 310                 315                 320 


Pro Gln Pro Gln Pro Gln Pro Gln Pro Gln Pro Gln Ser Gln Pro Gln 
                325                 330                 335     


Pro Gln Pro Gln Pro Lys Pro Gln Pro Gln Gln Leu His Pro Tyr Pro 
            340                 345                 350         


His Pro His Pro His Pro His Ser His Pro His Ser His Pro His Pro 
        355                 360                 365             


His Pro His Pro His Pro His Gln Ile Pro His Pro His Pro Gln Pro 
    370                 375                 380                 


His Ser Gln Pro His Gly His Arg Leu Leu Arg Ser Thr Ser Asn Ser 
385                 390                 395                 400 


Ala 
    


<210>  2
<211>  405
<212>  PRT
<213>  Mus musculus

<400>  2

Met Arg Arg Thr Pro Ala Ala Glu Arg Leu Ser Glu Leu Gly Phe Pro 
1               5                   10                  15      


Pro Arg Arg Gly Arg Gln Glu Pro Pro Phe Pro Leu Gly Val Thr Arg 
            20                  25                  30          


Gly Trp Gly Gly Trp Pro Ile Glu Lys Arg Arg Glu Gly Pro Arg Pro 
        35                  40                  45              


Val Pro Phe Ser Glu Arg Ser Pro Glu Asp Gly Arg Glu Gln Pro Ala 
    50                  55                  60                  


His Gly Ser Gly Ile Leu Trp Arg Val Arg Thr Arg Leu Ser Leu Cys 
65                  70                  75                  80  


Arg Asp Pro Glu Pro Pro Pro Pro Pro Pro Pro Leu Cys Leu Leu Arg 
                85                  90                  95      


Val Ser Leu Leu Cys Ala Leu Arg Ala Gly Gly Arg Gly Ser Arg Trp 
            100                 105                 110         


Gly Glu Asp Gly Ala Gly Leu Leu Leu Leu Pro Pro Ala Gly Ala Ser 
        115                 120                 125             


Gly Ser Leu Lys Ala Glu Arg Ser Ser Ser Thr Pro Tyr Ala Gly Arg 
    130                 135                 140                 


Met Leu Glu Asn Ser Gly Cys Lys Ala Leu Lys Glu Gly Val Leu Glu 
145                 150                 155                 160 


Lys Arg Ser Asp Gly Leu Leu Gln Leu Trp Lys Lys Lys Cys Cys Ile 
                165                 170                 175     


Leu Thr Glu Glu Gly Leu Leu Leu Ile Pro Pro Lys Gln Leu Gln Gln 
            180                 185                 190         


Gln Gln Gln Gln Gln Gln Pro Gly Gln Gly Thr Ala Glu Pro Ser Gln 
        195                 200                 205             


Pro Ser Gly Pro Thr Val Ala Ser Leu Glu Pro Pro Val Lys Leu Lys 
    210                 215                 220                 


Glu Leu His Phe Ser Asn Met Lys Thr Val Asp Cys Val Glu Arg Lys 
225                 230                 235                 240 


Gly Lys Tyr Met Tyr Phe Thr Val Val Met Thr Glu Gly Lys Glu Ile 
                245                 250                 255     


Asp Phe Arg Cys Pro Gln Asp Gln Gly Trp Asn Ala Glu Ile Thr Leu 
            260                 265                 270         


Gln Met Val Gln Tyr Lys Asn Arg Gln Ala Ile Leu Ala Val Lys Ser 
        275                 280                 285             


Thr Arg Gln Lys Gln Gln His Leu Val Gln Gln Gln Pro Pro Gln Thr 
    290                 295                 300                 


Gln Gln Ile Gln Pro Gln Pro Gln Pro Gln Ile Gln Pro Gln Pro Gln 
305                 310                 315                 320 


Pro Gln Ile Gln Pro Gln Pro Gln Pro Gln Pro Gln Pro Gln Pro Gln 
                325                 330                 335     


Pro Gln Pro Gln Pro Gln Pro Gln Gln Leu His Ser Tyr Pro His Pro 
            340                 345                 350         


His Pro His Pro Tyr Ser His Pro His Gln His Pro His Pro His Pro 
        355                 360                 365             


His Pro His Pro His Pro His Pro His Pro Tyr Gln Leu Gln His Ala 
    370                 375                 380                 


His Gln Pro Leu His Ser Gln Pro Gln Gly His Arg Leu Leu Arg Ser 
385                 390                 395                 400 


Thr Ser Asn Ser Ala 
                405 


<210>  3
<211>  638
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Ser Ala Leu Ala Gly Glu Asp Val Trp Arg Cys Pro Gly Cys Gly 
1               5                   10                  15      


Asp His Ile Ala Pro Ser Gln Ile Trp Tyr Arg Thr Val Asn Glu Thr 
            20                  25                  30          


Trp His Gly Ser Cys Phe Arg Cys Ser Glu Cys Gln Asp Ser Leu Thr 
        35                  40                  45              


Asn Trp Tyr Tyr Glu Lys Asp Gly Lys Leu Tyr Cys Pro Lys Asp Tyr 
    50                  55                  60                  


Trp Gly Lys Phe Gly Glu Phe Cys His Gly Cys Ser Leu Leu Met Thr 
65                  70                  75                  80  


Gly Pro Phe Met Val Ala Gly Glu Phe Lys Tyr His Pro Glu Cys Phe 
                85                  90                  95      


Ala Cys Met Ser Cys Lys Val Ile Ile Glu Asp Gly Asp Ala Tyr Ala 
            100                 105                 110         


Leu Val Gln His Ala Thr Leu Tyr Cys Gly Lys Cys His Asn Glu Val 
        115                 120                 125             


Val Leu Ala Pro Met Phe Glu Arg Leu Ser Thr Glu Ser Val Gln Glu 
    130                 135                 140                 


Gln Leu Pro Tyr Ser Val Thr Leu Ile Ser Met Pro Ala Thr Thr Glu 
145                 150                 155                 160 


Gly Arg Arg Gly Phe Ser Val Ser Val Glu Ser Ala Cys Ser Asn Tyr 
                165                 170                 175     


Ala Thr Thr Val Gln Val Lys Glu Val Asn Arg Met His Ile Ser Pro 
            180                 185                 190         


Asn Asn Arg Asn Ala Ile His Pro Gly Asp Arg Ile Leu Glu Ile Asn 
        195                 200                 205             


Gly Thr Pro Val Arg Thr Leu Arg Val Glu Glu Val Glu Asp Ala Ile 
    210                 215                 220                 


Ser Gln Thr Ser Gln Thr Leu Gln Leu Leu Ile Glu His Asp Pro Val 
225                 230                 235                 240 


Ser Gln Arg Leu Asp Gln Leu Arg Leu Glu Ala Arg Leu Ala Pro His 
                245                 250                 255     


Met Gln Asn Ala Gly His Pro His Ala Leu Ser Thr Leu Asp Thr Lys 
            260                 265                 270         


Glu Asn Leu Glu Gly Thr Leu Arg Arg Arg Ser Leu Arg Arg Ser Asn 
        275                 280                 285             


Ser Ile Ser Lys Ser Pro Gly Pro Ser Ser Pro Lys Glu Pro Leu Leu 
    290                 295                 300                 


Phe Ser Arg Asp Ile Ser Arg Ser Glu Ser Leu Arg Cys Ser Ser Ser 
305                 310                 315                 320 


Tyr Ser Gln Gln Ile Phe Arg Pro Cys Asp Leu Ile His Gly Glu Val 
                325                 330                 335     


Leu Gly Lys Gly Phe Phe Gly Gln Ala Ile Lys Val Thr His Lys Ala 
            340                 345                 350         


Thr Gly Lys Val Met Val Met Lys Glu Leu Ile Arg Cys Asp Glu Glu 
        355                 360                 365             


Thr Gln Lys Thr Phe Leu Thr Glu Val Lys Val Met Arg Ser Leu Asp 
    370                 375                 380                 


His Pro Asn Val Leu Lys Phe Ile Gly Val Leu Tyr Lys Asp Lys Lys 
385                 390                 395                 400 


Leu Asn Leu Leu Thr Glu Tyr Ile Glu Gly Gly Thr Leu Lys Asp Phe 
                405                 410                 415     


Leu Arg Ser Met Asp Pro Phe Pro Trp Gln Gln Lys Val Arg Phe Ala 
            420                 425                 430         


Lys Gly Ile Ala Ser Gly Met Ala Tyr Leu His Ser Met Cys Ile Ile 
        435                 440                 445             


His Arg Asp Leu Asn Ser His Asn Cys Leu Ile Lys Leu Asp Lys Thr 
    450                 455                 460                 


Val Val Val Ala Asp Phe Gly Leu Ser Arg Leu Ile Val Glu Glu Arg 
465                 470                 475                 480 


Lys Arg Ala Pro Met Glu Lys Ala Thr Thr Lys Lys Arg Thr Leu Arg 
                485                 490                 495     


Lys Asn Asp Arg Lys Lys Arg Tyr Thr Val Val Gly Asn Pro Tyr Trp 
            500                 505                 510         


Met Ala Pro Glu Met Leu Asn Gly Lys Ser Tyr Asp Glu Thr Val Asp 
        515                 520                 525             


Ile Phe Ser Phe Gly Ile Val Leu Cys Glu Ile Ile Gly Gln Val Tyr 
    530                 535                 540                 


Ala Asp Pro Asp Cys Leu Pro Arg Thr Leu Asp Phe Gly Leu Asn Val 
545                 550                 555                 560 


Lys Leu Phe Trp Glu Lys Phe Val Pro Thr Asp Cys Pro Pro Ala Phe 
                565                 570                 575     


Phe Pro Leu Ala Ala Ile Cys Cys Arg Leu Glu Pro Glu Ser Arg Pro 
            580                 585                 590         


Ala Phe Ser Lys Leu Glu Asp Ser Phe Glu Ala Leu Ser Leu Tyr Leu 
        595                 600                 605             


Gly Glu Leu Gly Ile Pro Leu Pro Ala Glu Leu Glu Glu Leu Asp His 
    610                 615                 620                 


Thr Val Ser Met Gln Tyr Gly Leu Thr Arg Asp Ser Pro Pro 
625                 630                 635             


<210>  4
<211>  451
<212>  PRT
<213>  Mus musculus

<400>  4

Met His Ile Ser Pro Asn Asn Arg Asn Ala Ile His Pro Gly Asp Arg 
1               5                   10                  15      


Ile Leu Glu Ile Asn Gly Thr Pro Val Arg Thr Leu Arg Val Glu Glu 
            20                  25                  30          


Val Glu Asp Ala Ile Lys Gln Thr Ser Gln Thr Leu Gln Leu Leu Ile 
        35                  40                  45              


Glu His Asp Pro Val Pro Gln Arg Leu Asp Gln Leu Arg Leu Asp Ala 
    50                  55                  60                  


Arg Leu Pro Pro His Met Gln Ser Thr Gly His Thr Leu Met Leu Ser 
65                  70                  75                  80  


Thr Leu Asp Thr Lys Glu Asn Gln Glu Gly Thr Leu Arg Arg Arg Ser 
                85                  90                  95      


Leu Arg Arg Ser Asn Ser Ile Ser Lys Ser Pro Gly Pro Ser Ser Pro 
            100                 105                 110         


Lys Glu Pro Leu Leu Leu Ser Arg Asp Ile Ser Arg Ser Glu Ser Leu 
        115                 120                 125             


Arg Cys Ser Ser Ser Tyr Ser Gln Gln Ile Phe Arg Pro Cys Asp Leu 
    130                 135                 140                 


Ile His Gly Glu Val Leu Gly Lys Gly Phe Phe Gly Gln Ala Ile Lys 
145                 150                 155                 160 


Val Thr His Lys Ala Thr Gly Lys Val Met Val Met Lys Glu Leu Ile 
                165                 170                 175     


Arg Cys Asp Glu Glu Thr Gln Lys Thr Phe Leu Thr Glu Val Lys Val 
            180                 185                 190         


Met Arg Ser Leu Asp His Pro Asn Val Leu Lys Phe Ile Gly Val Leu 
        195                 200                 205             


Tyr Lys Asp Lys Lys Leu Asn Leu Leu Thr Glu Tyr Ile Glu Gly Gly 
    210                 215                 220                 


Thr Leu Lys Asp Phe Leu Arg Ser Val Asp Pro Phe Pro Trp Gln Gln 
225                 230                 235                 240 


Lys Val Arg Phe Ala Lys Gly Ile Ser Ser Gly Met Ala Tyr Leu His 
                245                 250                 255     


Ser Met Cys Ile Ile His Arg Asp Leu Asn Ser His Asn Cys Leu Ile 
            260                 265                 270         


Lys Leu Asp Lys Thr Val Val Val Ala Asp Phe Gly Leu Ser Arg Leu 
        275                 280                 285             


Ile Val Glu Glu Arg Lys Arg Pro Pro Val Glu Lys Ala Thr Thr Lys 
    290                 295                 300                 


Lys Arg Thr Leu Arg Lys Ser Asp Arg Lys Lys Arg Tyr Thr Val Val 
305                 310                 315                 320 


Gly Asn Pro Tyr Trp Met Ala Pro Glu Met Leu Asn Gly Lys Ser Tyr 
                325                 330                 335     


Asp Glu Thr Val Asp Val Phe Ser Phe Gly Ile Val Leu Cys Glu Ile 
            340                 345                 350         


Ile Gly Gln Val Tyr Ala Asp Pro Asp Cys Leu Pro Arg Thr Leu Asp 
        355                 360                 365             


Phe Gly Leu Asn Val Lys Leu Phe Trp Glu Lys Phe Val Pro Thr Asp 
    370                 375                 380                 


Cys Pro Pro Ala Phe Phe Pro Leu Ala Ala Ile Cys Cys Lys Leu Glu 
385                 390                 395                 400 


Pro Glu Ser Arg Pro Ala Phe Ser Lys Leu Glu Asp Ser Phe Glu Ala 
                405                 410                 415     


Leu Ser Leu Phe Leu Gly Glu Leu Ala Ile Pro Leu Pro Ala Glu Leu 
            420                 425                 430         


Glu Asp Leu Asp His Thr Val Ser Met Glu Tyr Gly Leu Thr Arg Asp 
        435                 440                 445             


Ser Pro Pro 
    450     


<210>  5
<211>  403
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Asp Arg Ser Lys Glu Asn Cys Ile Ser Gly Pro Val Lys Ala Thr 
1               5                   10                  15      


Ala Pro Val Gly Gly Pro Lys Arg Val Leu Val Thr Gln Gln Phe Pro 
            20                  25                  30          


Cys Gln Asn Pro Leu Pro Val Asn Ser Gly Gln Ala Gln Arg Val Leu 
        35                  40                  45              


Cys Pro Ser Asn Ser Ser Gln Arg Val Pro Leu Gln Ala Gln Lys Leu 
    50                  55                  60                  


Val Ser Ser His Lys Pro Val Gln Asn Gln Lys Gln Lys Gln Leu Gln 
65                  70                  75                  80  


Ala Thr Ser Val Pro His Pro Val Ser Arg Pro Leu Asn Asn Thr Gln 
                85                  90                  95      


Lys Ser Lys Gln Pro Leu Pro Ser Ala Pro Glu Asn Asn Pro Glu Glu 
            100                 105                 110         


Glu Leu Ala Ser Lys Gln Lys Asn Glu Glu Ser Lys Lys Arg Gln Trp 
        115                 120                 125             


Ala Leu Glu Asp Phe Glu Ile Gly Arg Pro Leu Gly Lys Gly Lys Phe 
    130                 135                 140                 


Gly Asn Val Tyr Leu Ala Arg Glu Lys Gln Ser Lys Phe Ile Leu Ala 
145                 150                 155                 160 


Leu Lys Val Leu Phe Lys Ala Gln Leu Glu Lys Ala Gly Val Glu His 
                165                 170                 175     


Gln Leu Arg Arg Glu Val Glu Ile Gln Ser His Leu Arg His Pro Asn 
            180                 185                 190         


Ile Leu Arg Leu Tyr Gly Tyr Phe His Asp Ala Thr Arg Val Tyr Leu 
        195                 200                 205             


Ile Leu Glu Tyr Ala Pro Leu Gly Thr Val Tyr Arg Glu Leu Gln Lys 
    210                 215                 220                 


Leu Ser Lys Phe Asp Glu Gln Arg Thr Ala Thr Tyr Ile Thr Glu Leu 
225                 230                 235                 240 


Ala Asn Ala Leu Ser Tyr Cys His Ser Lys Arg Val Ile His Arg Asp 
                245                 250                 255     


Ile Lys Pro Glu Asn Leu Leu Leu Gly Ser Ala Gly Glu Leu Lys Ile 
            260                 265                 270         


Ala Asp Phe Gly Trp Ser Val His Ala Pro Ser Ser Arg Arg Thr Thr 
        275                 280                 285             


Leu Cys Gly Thr Leu Asp Tyr Leu Pro Pro Glu Met Ile Glu Gly Arg 
    290                 295                 300                 


Met His Asp Glu Lys Val Asp Leu Trp Ser Leu Gly Val Leu Cys Tyr 
305                 310                 315                 320 


Glu Phe Leu Val Gly Lys Pro Pro Phe Glu Ala Asn Thr Tyr Gln Glu 
                325                 330                 335     


Thr Tyr Lys Arg Ile Ser Arg Val Glu Phe Thr Phe Pro Asp Phe Val 
            340                 345                 350         


Thr Glu Gly Ala Arg Asp Leu Ile Ser Arg Leu Leu Lys His Asn Pro 
        355                 360                 365             


Ser Gln Arg Pro Met Leu Arg Glu Val Leu Glu His Pro Trp Ile Thr 
    370                 375                 380                 


Ala Asn Ser Ser Lys Pro Ser Asn Cys Gln Asn Lys Glu Ser Ala Ser 
385                 390                 395                 400 


Lys Gln Ser 
            


<210>  6
<211>  395
<212>  PRT
<213>  Mus musculus

<400>  6

Met Asp Arg Cys Lys Glu Asn Cys Val Ser Arg Pro Val Lys Thr Thr 
1               5                   10                  15      


Val Pro Phe Gly Pro Lys Arg Val Leu Val Thr Glu Gln Ile Pro Ser 
            20                  25                  30          


Gln Asn Leu Gly Ser Ala Ser Ser Gly Gln Ala Gln Arg Val Leu Cys 
        35                  40                  45              


Pro Ser Asn Ser Gln Arg Val Pro Ser Gln Ala Gln Lys Leu Gly Ala 
    50                  55                  60                  


Gly Gln Lys Pro Ala Pro Lys Gln Leu Pro Ala Ala Ser Val Pro Arg 
65                  70                  75                  80  


Pro Val Ser Arg Leu Asn Asn Pro Gln Lys Asn Glu Gln Pro Ala Ala 
                85                  90                  95      


Ser Gly Asn Asp Ser Glu Lys Glu Gln Ala Ser Leu Gln Lys Thr Glu 
            100                 105                 110         


Asp Thr Lys Lys Arg Gln Trp Thr Leu Glu Asp Phe Asp Ile Gly Arg 
        115                 120                 125             


Pro Leu Gly Lys Gly Lys Phe Gly Asn Val Tyr Leu Ala Arg Glu Arg 
    130                 135                 140                 


Gln Ser Lys Phe Ile Leu Ala Leu Lys Val Leu Phe Lys Thr Gln Leu 
145                 150                 155                 160 


Glu Lys Ala Asn Val Glu His Gln Leu Arg Arg Glu Val Glu Ile Gln 
                165                 170                 175     


Ser His Leu Arg His Pro Asn Ile Leu Arg Leu Tyr Gly Tyr Phe His 
            180                 185                 190         


Asp Ala Thr Arg Val Tyr Leu Ile Leu Glu Tyr Ala Pro Leu Gly Thr 
        195                 200                 205             


Val Tyr Arg Glu Leu Gln Lys Leu Ser Lys Phe Asp Glu Gln Arg Thr 
    210                 215                 220                 


Ala Thr Tyr Ile Thr Glu Leu Ala Asn Ala Leu Ser Tyr Cys His Ser 
225                 230                 235                 240 


Lys Arg Val Ile His Arg Asp Ile Lys Pro Glu Asn Leu Leu Leu Gly 
                245                 250                 255     


Ser Asn Gly Glu Leu Lys Ile Ala Asp Phe Gly Trp Ser Val His Ala 
            260                 265                 270         


Pro Ser Ser Arg Arg Thr Thr Met Cys Gly Thr Leu Asp Tyr Leu Pro 
        275                 280                 285             


Pro Glu Met Ile Glu Gly Arg Met His Asp Glu Lys Val Asp Leu Trp 
    290                 295                 300                 


Ser Leu Gly Val Leu Cys Tyr Glu Phe Leu Val Gly Met Pro Pro Phe 
305                 310                 315                 320 


Glu Ala His Thr Tyr Gln Glu Thr Tyr Arg Arg Ile Ser Arg Val Glu 
                325                 330                 335     


Phe Thr Phe Pro Asp Phe Val Thr Glu Gly Ala Arg Asp Leu Ile Ser 
            340                 345                 350         


Arg Leu Leu Lys His Asn Ala Ser Gln Arg Leu Thr Leu Ala Glu Val 
        355                 360                 365             


Leu Glu His Pro Trp Ile Lys Ala Asn Ser Ser Lys Pro Pro Thr Gly 
    370                 375                 380                 


His Thr Ser Lys Glu Pro Thr Ser Lys Ser Ser 
385                 390                 395 


<210>  7
<211>  395
<212>  PRT
<213>  artificial sequence

<220>
<223>  mutant Aurora A kinase capable of accepting orthogonal ATP 
       analogs

<400>  7

Met Asp Arg Cys Lys Glu Asn Cys Val Ser Arg Pro Val Lys Thr Thr 
1               5                   10                  15      


Val Pro Phe Gly Pro Lys Arg Val Leu Val Thr Glu Gln Ile Pro Ser 
            20                  25                  30          


Gln Asn Leu Gly Ser Ala Ser Ser Gly Gln Ala Gln Arg Val Leu Cys 
        35                  40                  45              


Pro Ser Asn Ser Gln Arg Val Pro Ser Gln Ala Gln Lys Leu Gly Ala 
    50                  55                  60                  


Gly Gln Lys Pro Ala Pro Lys Gln Leu Pro Ala Ala Ser Val Pro Arg 
65                  70                  75                  80  


Pro Val Ser Arg Leu Asn Asn Pro Gln Lys Asn Glu Gln Pro Ala Ala 
                85                  90                  95      


Ser Gly Asn Asp Ser Glu Lys Glu Gln Ala Ser Leu Gln Lys Thr Glu 
            100                 105                 110         


Asp Thr Lys Lys Arg Gln Trp Thr Leu Glu Asp Phe Asp Ile Gly Arg 
        115                 120                 125             


Pro Leu Gly Lys Gly Lys Phe Gly Asn Val Tyr Leu Ala Arg Glu Arg 
    130                 135                 140                 


Gln Ser Lys Phe Ile Leu Ala Leu Lys Val Leu Phe Lys Thr Gln Leu 
145                 150                 155                 160 


Glu Lys Ala Asn Val Glu His Gln Leu Arg Arg Glu Val Glu Ile Gln 
                165                 170                 175     


Ser His Leu Arg His Pro Asn Ile Val Arg Leu Tyr Gly Tyr Phe His 
            180                 185                 190         


Asp Ala Thr Arg Val Tyr Leu Ile Gly Glu Tyr Ala Pro Leu Gly Thr 
        195                 200                 205             


Val Tyr Arg Glu Leu Gln Lys Leu Ser Lys Phe Asp Glu Gln Arg Thr 
    210                 215                 220                 


Ala Thr Tyr Ile Thr Glu Leu Ala Asn Ala Leu Ser Tyr Cys His Ser 
225                 230                 235                 240 


Lys Arg Val Ile His Arg Asp Ile Lys Pro Glu Asn Leu Leu Leu Gly 
                245                 250                 255     


Ser Asn Gly Glu Leu Lys Ile Ala Asp Phe Gly Trp Ser Val His Ala 
            260                 265                 270         


Pro Ser Ser Arg Arg Thr Thr Met Cys Gly Thr Leu Asp Tyr Leu Pro 
        275                 280                 285             


Pro Glu Met Ile Glu Gly Arg Met His Asp Glu Lys Val Asp Leu Trp 
    290                 295                 300                 


Ser Leu Gly Val Leu Cys Tyr Glu Phe Leu Val Gly Met Pro Pro Phe 
305                 310                 315                 320 


Glu Ala His Thr Tyr Gln Glu Thr Tyr Arg Arg Ile Ser Arg Val Glu 
                325                 330                 335     


Phe Thr Phe Pro Asp Phe Val Thr Glu Gly Ala Arg Asp Leu Ile Ser 
            340                 345                 350         


Arg Leu Leu Lys His Asn Ala Ser Gln Arg Leu Thr Leu Ala Glu Val 
        355                 360                 365             


Leu Glu His Pro Trp Ile Lys Ala Asn Ser Ser Lys Pro Pro Thr Gly 
    370                 375                 380                 


His Thr Ser Lys Glu Pro Thr Ser Lys Ser Ser 
385                 390                 395 


<210>  8
<211>  405
<212>  PRT
<213>  artificial sequence

<220>
<223>  modified PHLDA sequence with Aurora a kinse sites removed

<400>  8

Met Arg Arg Thr Pro Ala Ala Glu Arg Leu Ser Glu Leu Gly Phe Pro 
1               5                   10                  15      


Pro Arg Arg Gly Arg Gln Glu Pro Pro Phe Pro Leu Gly Val Thr Arg 
            20                  25                  30          


Gly Trp Gly Gly Trp Pro Ile Glu Lys Arg Arg Glu Gly Pro Arg Pro 
        35                  40                  45              


Val Pro Phe Ser Glu Arg Ser Pro Glu Asp Gly Arg Glu Gln Pro Ala 
    50                  55                  60                  


His Gly Ser Gly Ile Leu Trp Arg Val Arg Thr Arg Leu Ser Leu Cys 
65                  70                  75                  80  


Arg Asp Pro Glu Pro Pro Pro Pro Pro Pro Pro Leu Cys Leu Leu Arg 
                85                  90                  95      


Val Ala Leu Leu Cys Ala Leu Arg Ala Gly Gly Arg Gly Ser Arg Trp 
            100                 105                 110         


Gly Glu Asp Gly Ala Gly Leu Leu Leu Leu Pro Pro Ala Gly Ala Ser 
        115                 120                 125             


Gly Ser Leu Lys Ala Glu Arg Ser Ser Ser Thr Pro Tyr Ala Gly Arg 
    130                 135                 140                 


Met Leu Glu Asn Ser Gly Cys Lys Ala Leu Lys Glu Gly Val Leu Glu 
145                 150                 155                 160 


Lys Arg Ser Asp Gly Leu Leu Gln Leu Trp Lys Lys Lys Cys Cys Ile 
                165                 170                 175     


Leu Thr Glu Glu Gly Leu Leu Leu Ile Pro Pro Lys Gln Leu Gln Gln 
            180                 185                 190         


Gln Gln Gln Gln Gln Gln Pro Gly Gln Gly Thr Ala Glu Pro Ser Gln 
        195                 200                 205             


Pro Ser Gly Pro Thr Val Ala Ser Leu Glu Pro Pro Val Lys Leu Lys 
    210                 215                 220                 


Glu Leu His Phe Ser Asn Met Lys Thr Val Asp Cys Val Glu Arg Lys 
225                 230                 235                 240 


Gly Lys Tyr Met Tyr Phe Thr Val Val Met Thr Glu Gly Lys Glu Ile 
                245                 250                 255     


Asp Phe Arg Cys Pro Gln Asp Gln Gly Trp Asn Ala Glu Ile Thr Leu 
            260                 265                 270         


Gln Met Val Gln Tyr Lys Asn Arg Gln Ala Ile Leu Ala Val Lys Ser 
        275                 280                 285             


Thr Arg Gln Lys Gln Gln His Leu Val Gln Gln Gln Pro Pro Gln Thr 
    290                 295                 300                 


Gln Gln Ile Gln Pro Gln Pro Gln Pro Gln Ile Gln Pro Gln Pro Gln 
305                 310                 315                 320 


Pro Gln Ile Gln Pro Gln Pro Gln Pro Gln Pro Gln Pro Gln Pro Gln 
                325                 330                 335     


Pro Gln Pro Gln Pro Gln Pro Gln Gln Leu His Ser Tyr Pro His Pro 
            340                 345                 350         


His Pro His Pro Tyr Ser His Pro His Gln His Pro His Pro His Pro 
        355                 360                 365             


His Pro His Pro His Pro His Pro His Pro Tyr Gln Leu Gln His Ala 
    370                 375                 380                 


His Gln Pro Leu His Ser Gln Pro Gln Gly His Arg Leu Leu Arg Ser 
385                 390                 395                 400 


Thr Ser Asn Ser Ala 
                405 


<210>  9
<211>  638
<212>  PRT
<213>  artificial sequence

<220>
<223>  modified LIMK2 sequence with Aurora a kinase sites remmoved

<400>  9

Met Ser Ala Leu Ala Gly Glu Asp Val Trp Arg Cys Pro Gly Cys Gly 
1               5                   10                  15      


Asp His Ile Ala Pro Ser Gln Ile Trp Tyr Arg Thr Val Asn Glu Thr 
            20                  25                  30          


Trp His Gly Ser Cys Phe Arg Cys Ser Glu Cys Gln Asp Ser Leu Thr 
        35                  40                  45              


Asn Trp Tyr Tyr Glu Lys Asp Gly Lys Leu Tyr Cys Pro Lys Asp Tyr 
    50                  55                  60                  


Trp Gly Lys Phe Gly Glu Phe Cys His Gly Cys Ser Leu Leu Met Thr 
65                  70                  75                  80  


Gly Pro Phe Met Val Ala Gly Glu Phe Lys Tyr His Pro Glu Cys Phe 
                85                  90                  95      


Ala Cys Met Ser Cys Lys Val Ile Ile Glu Asp Gly Asp Ala Tyr Ala 
            100                 105                 110         


Leu Val Gln His Ala Thr Leu Tyr Cys Gly Lys Cys His Asn Glu Val 
        115                 120                 125             


Val Leu Ala Pro Met Phe Glu Arg Leu Ser Thr Glu Ser Val Gln Glu 
    130                 135                 140                 


Gln Leu Pro Tyr Ser Val Thr Leu Ile Ser Met Pro Ala Thr Thr Glu 
145                 150                 155                 160 


Gly Arg Arg Gly Phe Ser Val Ser Val Glu Ser Ala Cys Ser Asn Tyr 
                165                 170                 175     


Ala Thr Thr Val Gln Val Lys Glu Val Asn Arg Met His Ile Ser Pro 
            180                 185                 190         


Asn Asn Arg Asn Ala Ile His Pro Gly Asp Arg Ile Leu Glu Ile Asn 
        195                 200                 205             


Gly Thr Pro Val Arg Thr Leu Arg Val Glu Glu Val Glu Asp Ala Ile 
    210                 215                 220                 


Ser Gln Thr Ser Gln Thr Leu Gln Leu Leu Ile Glu His Asp Pro Val 
225                 230                 235                 240 


Ser Gln Arg Leu Asp Gln Leu Arg Leu Glu Ala Arg Leu Ala Pro His 
                245                 250                 255     


Met Gln Asn Ala Gly His Pro His Ala Leu Ser Thr Leu Asp Thr Lys 
            260                 265                 270         


Glu Asn Leu Glu Gly Thr Leu Arg Arg Arg Ala Leu Arg Arg Ser Asn 
        275                 280                 285             


Ser Ile Ser Lys Ser Pro Gly Pro Ser Ser Pro Lys Glu Pro Leu Leu 
    290                 295                 300                 


Phe Ser Arg Asp Ile Ser Arg Ser Glu Ser Leu Arg Cys Ser Ser Ser 
305                 310                 315                 320 


Tyr Ser Gln Gln Ile Phe Arg Pro Cys Asp Leu Ile His Gly Glu Val 
                325                 330                 335     


Leu Gly Lys Gly Phe Phe Gly Gln Ala Ile Lys Val Thr His Lys Ala 
            340                 345                 350         


Thr Gly Lys Val Met Val Met Lys Glu Leu Ile Arg Cys Asp Glu Glu 
        355                 360                 365             


Thr Gln Lys Thr Phe Leu Thr Glu Val Lys Val Met Arg Ser Leu Asp 
    370                 375                 380                 


His Pro Asn Val Leu Lys Phe Ile Gly Val Leu Tyr Lys Asp Lys Lys 
385                 390                 395                 400 


Leu Asn Leu Leu Thr Glu Tyr Ile Glu Gly Gly Thr Leu Lys Asp Phe 
                405                 410                 415     


Leu Arg Ser Met Asp Pro Phe Pro Trp Gln Gln Lys Val Arg Phe Ala 
            420                 425                 430         


Lys Gly Ile Ala Ser Gly Met Ala Tyr Leu His Ser Met Cys Ile Ile 
        435                 440                 445             


His Arg Asp Leu Asn Ser His Asn Cys Leu Ile Lys Leu Asp Lys Thr 
    450                 455                 460                 


Val Val Val Ala Asp Phe Gly Leu Ser Arg Leu Ile Val Glu Glu Arg 
465                 470                 475                 480 


Lys Arg Ala Pro Met Glu Lys Ala Thr Thr Lys Lys Arg Ala Leu Arg 
                485                 490                 495     


Lys Asn Asp Arg Lys Lys Arg Tyr Ala Val Val Gly Asn Pro Tyr Trp 
            500                 505                 510         


Met Ala Pro Glu Met Leu Asn Gly Lys Ser Tyr Asp Glu Thr Val Asp 
        515                 520                 525             


Ile Phe Ser Phe Gly Ile Val Leu Cys Glu Ile Ile Gly Gln Val Tyr 
    530                 535                 540                 


Ala Asp Pro Asp Cys Leu Pro Arg Thr Leu Asp Phe Gly Leu Asn Val 
545                 550                 555                 560 


Lys Leu Phe Trp Glu Lys Phe Val Pro Thr Asp Cys Pro Pro Ala Phe 
                565                 570                 575     


Phe Pro Leu Ala Ala Ile Cys Cys Arg Leu Glu Pro Glu Ser Arg Pro 
            580                 585                 590         


Ala Phe Ser Lys Leu Glu Asp Ser Phe Glu Ala Leu Ser Leu Tyr Leu 
        595                 600                 605             


Gly Glu Leu Gly Ile Pro Leu Pro Ala Glu Leu Glu Glu Leu Asp His 
    610                 615                 620                 


Thr Val Ser Met Gln Tyr Gly Leu Thr Arg Asp Ser Pro Pro 
625                 630                 635             


<210>  10
<211>  747
<212>  PRT
<213>  Homo sapiens

<400>  10

Met Ser Gln Val Lys Ser Ser Tyr Ser Tyr Asp Ala Pro Ser Asp Phe 
1               5                   10                  15      


Ile Asn Phe Ser Ser Leu Asp Asp Glu Gly Asp Thr Gln Asn Ile Asp 
            20                  25                  30          


Ser Trp Phe Glu Glu Lys Ala Asn Leu Glu Asn Lys Leu Leu Gly Lys 
        35                  40                  45              


Asn Gly Thr Gly Gly Leu Phe Gln Gly Lys Thr Pro Leu Arg Lys Ala 
    50                  55                  60                  


Asn Leu Gln Gln Ala Ile Val Thr Pro Leu Lys Pro Val Asp Asn Thr 
65                  70                  75                  80  


Tyr Tyr Lys Glu Ala Glu Lys Glu Asn Leu Val Glu Gln Ser Ile Pro 
                85                  90                  95      


Ser Asn Ala Cys Ser Ser Leu Glu Val Glu Ala Ala Ile Ser Arg Lys 
            100                 105                 110         


Thr Pro Ala Gln Pro Gln Arg Arg Ser Leu Arg Leu Ser Ala Gln Lys 
        115                 120                 125             


Asp Leu Glu Gln Lys Glu Lys His His Val Lys Met Lys Ala Lys Arg 
    130                 135                 140                 


Cys Ala Thr Pro Val Ile Ile Asp Glu Ile Leu Pro Ser Lys Lys Met 
145                 150                 155                 160 


Lys Val Ser Asn Asn Lys Lys Lys Pro Glu Glu Glu Gly Ser Ala His 
                165                 170                 175     


Gln Asp Thr Ala Glu Lys Asn Ala Ser Ser Pro Glu Lys Ala Lys Gly 
            180                 185                 190         


Arg His Thr Val Pro Cys Met Pro Pro Ala Lys Gln Lys Phe Leu Lys 
        195                 200                 205             


Ser Thr Glu Glu Gln Glu Leu Glu Lys Ser Met Lys Met Gln Gln Glu 
    210                 215                 220                 


Val Val Glu Met Arg Lys Lys Asn Glu Glu Phe Lys Lys Leu Ala Leu 
225                 230                 235                 240 


Ala Gly Ile Gly Gln Pro Val Lys Lys Ser Val Ser Gln Val Thr Lys 
                245                 250                 255     


Ser Val Asp Phe His Phe Arg Thr Asp Glu Arg Ile Lys Gln His Pro 
            260                 265                 270         


Lys Asn Gln Glu Glu Tyr Lys Glu Val Asn Phe Thr Ser Glu Leu Arg 
        275                 280                 285             


Lys His Pro Ser Ser Pro Ala Arg Val Thr Lys Gly Cys Thr Ile Val 
    290                 295                 300                 


Lys Pro Phe Asn Leu Ser Gln Gly Lys Lys Arg Thr Phe Asp Glu Thr 
305                 310                 315                 320 


Val Ser Thr Tyr Val Pro Leu Ala Gln Gln Val Glu Asp Phe His Lys 
                325                 330                 335     


Arg Thr Pro Asn Arg Tyr His Leu Arg Ser Lys Lys Asp Asp Ile Asn 
            340                 345                 350         


Leu Leu Pro Ser Lys Ser Ser Val Thr Lys Ile Cys Arg Asp Pro Gln 
        355                 360                 365             


Thr Pro Val Leu Gln Thr Lys His Arg Ala Arg Ala Val Thr Cys Lys 
    370                 375                 380                 


Ser Thr Ala Glu Leu Glu Ala Glu Glu Leu Glu Lys Leu Gln Gln Tyr 
385                 390                 395                 400 


Lys Phe Lys Ala Arg Glu Leu Asp Pro Arg Ile Leu Glu Gly Gly Pro 
                405                 410                 415     


Ile Leu Pro Lys Lys Pro Pro Val Lys Pro Pro Thr Glu Pro Ile Gly 
            420                 425                 430         


Phe Asp Leu Glu Ile Glu Lys Arg Ile Gln Glu Arg Glu Ser Lys Lys 
        435                 440                 445             


Lys Thr Glu Asp Glu His Phe Glu Phe His Ser Arg Pro Cys Pro Thr 
    450                 455                 460                 


Lys Ile Leu Glu Asp Val Val Gly Val Pro Glu Lys Lys Val Leu Pro 
465                 470                 475                 480 


Ile Thr Val Pro Lys Ser Pro Ala Phe Ala Leu Lys Asn Arg Ile Arg 
                485                 490                 495     


Met Pro Thr Lys Glu Asp Glu Glu Glu Asp Glu Pro Val Val Ile Lys 
            500                 505                 510         


Ala Gln Pro Val Pro His Tyr Gly Val Pro Phe Lys Pro Gln Ile Pro 
        515                 520                 525             


Glu Ala Arg Thr Val Glu Ile Cys Pro Phe Ser Phe Asp Ser Arg Asp 
    530                 535                 540                 


Lys Glu Arg Gln Leu Gln Lys Glu Lys Lys Ile Lys Glu Leu Gln Lys 
545                 550                 555                 560 


Gly Glu Val Pro Lys Phe Lys Ala Leu Pro Leu Pro His Phe Asp Thr 
                565                 570                 575     


Ile Asn Leu Pro Glu Lys Lys Val Lys Asn Val Thr Gln Ile Glu Pro 
            580                 585                 590         


Phe Cys Leu Glu Thr Asp Arg Arg Gly Ala Leu Lys Ala Gln Thr Trp 
        595                 600                 605             


Lys His Gln Leu Glu Glu Glu Leu Arg Gln Gln Lys Glu Ala Ala Cys 
    610                 615                 620                 


Phe Lys Ala Arg Pro Asn Thr Val Ile Ser Gln Glu Pro Phe Val Pro 
625                 630                 635                 640 


Lys Lys Glu Lys Lys Ser Val Ala Glu Gly Leu Ser Gly Ser Leu Val 
                645                 650                 655     


Gln Glu Pro Phe Gln Leu Ala Thr Glu Lys Arg Ala Lys Glu Arg Gln 
            660                 665                 670         


Glu Leu Glu Lys Arg Met Ala Glu Val Glu Ala Gln Lys Ala Gln Gln 
        675                 680                 685             


Leu Glu Glu Ala Arg Leu Gln Glu Glu Glu Gln Lys Lys Glu Glu Leu 
    690                 695                 700                 


Ala Arg Leu Arg Arg Glu Leu Val His Lys Ala Asn Pro Ile Arg Lys 
705                 710                 715                 720 


Tyr Gln Gly Leu Glu Ile Lys Ser Ser Asp Gln Pro Leu Thr Val Pro 
                725                 730                 735     


Val Ser Pro Lys Phe Ser Thr Arg Phe His Cys 
            740                 745         


<210>  11
<211>  58
<212>  DNA
<213>  artificial sequence

<220>
<223>  Aurora A primer

<400>  11
ccgggcacca cttggaacag tttatctcga gataaactgt tccaagtggt gctttttg         58


<210>  12
<211>  58
<212>  DNA
<213>  artificial sequence

<220>
<223>  Aurora A primer

<400>  12
aattcaaaaa gcaccacttg gaacagttta tctcgagata aactgttcca agtggtgc         58


<210>  13
<211>  58
<212>  DNA
<213>  artificial sequence

<220>
<223>  Aurora A primer

<400>  13
ccgggccaat gctcagagaa gtactctcga gagtacttct ctgagcattg gctttttg         58


<210>  14
<211>  58
<212>  DNA
<213>  artificial sequence

<220>
<223>  Aurora A primer

<400>  14
aattcaaaaa gccaatgctc agagaagtac tctcgagagt acttctctga gcattggc         58


<210>  15
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  PHLDA-1 primer

<400>  15
ccgggatggt gcagtacaag aatctcgaga ttcttgtact gcaccatctt tttg             54


<210>  16
<211>  52
<212>  DNA
<213>  artificial sequence

<220>
<223>  PHLDA-1 primer

<400>  16
aattcaaaaa gatggtgcag tacaagaatc tcgagattct tgtactgcac ca               52


<210>  17
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  PHLDA-1 primer

<400>  17
ccggtccgca tccacatcca catctcgaga tgtggatgtg gatgcggatt tttg             54


<210>  18
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  PHLDA-1 primer

<400>  18
aattcaaaaa tccgcatcca catccacatc tcgagatgtg gatgtggatg cgga             54


<210>  19
<211>  10
<212>  PRT
<213>  Homo sapiens

<400>  19

Ala Leu Arg Arg Ala Ser Leu Gly Ala Ala 
1               5                   10  


<210>  20
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer for LIMK2

<400>  20
ccggccaact ggtactatga gaactcgagt tctcatagta ccagttggtt tttg             54


<210>  21
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer for LIMK2

<400>  21
aattcaaaaa ccaactggta ctatgagaac tcgagttctc atagtaccag ttgg             54


<210>  22
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer for LIMK2

<400>  22
ccgggctatt cacagcagat cttctcgaga agatctgctg tgaatagctt tttg             54


<210>  23
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer for LIMK2

<400>  23
aattcaaaaa gctattcaca gcagatcttc tcgagaagat ctgctgtgaa tagc             54


<210>  24
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer for LIMK2

<400>  24
ccggcctgct gacagagtac attctcgaga atgtactctg tcagcaggtt tttg             54


<210>  25
<211>  54
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer for LIMK2

<400>  25
aattcaaaaa cctgctgaca gagtacattc tcgagaatgt actctgtcag cagg             54


