
                                SEQUENCE LISTING

<110> Exact Sciences Corporation
      Zou, Hongzhi
      Lidgard, Graham P.
      Domanico, Michael J.
      Allawi, Hatim

<120> Methylation Assay
  

<130> EXAS-002WO

<140> US 12/946,745       
<141> 2010-11-15  

<160> 19

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 207
<212> DNA
<213> Artificial Sequence

<220> 
<223> target methylated C6ORF150

<220> 
<221> misc_difference
<222> 16, 21, 34, 42, 52, 58, 72, 82, 88, 99, 107, 121, 126, 144,
148, 152, 154, 167, 169, 188, 190, 201, 207
<223> methylated cytosine residues

<400> 1
atggaatgtt aggggcgttt cgatggattt tatcgagttt tcggttgttt tcgaggtcgt 60
tttgtttaag gcgggaaagt tcggtttcgt taggaagtcg ggatttcggt agaaaaagag 120
cgtttcggat atttaggaga ggtcgttcgt tcgcgtaatt ggggttcgcg ttaaaaaggt 180
tttttagcgc gtttaggata cgtagtc                                     207

<210> 2
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic primer

<400> 2
gggatttcgg tagaaaaaga gcgt                                        24

<210> 3
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic primer

<400> 3
acctttttaa cgcgaacccc a                                           21

<210> 4
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic invasive oligonucleotide

<400> 4
tcggatattt aggagaggtg                                             20

<210> 5
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic flap probe

<220> 
<221> misc_difference
<222> 10, 14, 18, 20
<223> Methylated cytosine

<220> 
<221> misc_difference
<222> 21
<223> 3'-C6 hexanediol modified

<400> 5
gacgcggagc gttcgttcgc g                                           21

<210> 6
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic  FRET cassette

<220> 
<221> misc_difference
<222> 1
<223> 5'-FAM modified

<220> 
<221> misc_difference
<222> 3
<223> Nucleotide modified with quencher

<220> 
<221> misc_difference
<222> 35
<223> 3'-C6 hexanediol modified

<400> 6
tctagccggt tttccggctg agactccgcg tccgt                            35

<210> 7
<211> 136
<212> DNA
<213> Artificial Sequence

<220> 
<223> Target ZNF804B sequence

<220> 
<221> misc_difference
<222> 47, 74, 77, 80, 92, 123
<223> Methylated cytosine

<400> 7
ttaatttgtt tgttttattt gtggttgtat agtttatttt tgtaatcggt tggggagttg 60
ttgtttttgt taacgtcgtc gttagttaga gcgttgaaga aaagttgaag gttagtaggt 120
aacgaaagag taaaga                                                 136

<210> 8
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic primer

<400> 8
gtggttgtat agtttatttt tgtaatcggt                                  30

<210> 9
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic primer

<400> 9
accttcaact tttcttcaac gctc                                        24

<210> 10
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic invasive oligonucleotide

<400> 10
gggagttgtt gtttttgtta ag                                          22

<210> 11
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic flap probe

<220> 
<221> misc_difference
<222> 21
<223> 3'-C6 hexanediol modified

<220> 
<221> misc_difference
<222> 10, 13, 16
<223> Methylated cytosine

<400> 11
gacgcggagc gtcgtcgtta g                                           21

<210> 12
<211> 114
<212> DNA
<213> Artificial Sequence

<220> 
<223> Target vimentin sequence

<220> 
<221> misc_difference
<222> 2, 10, 21, 32, 35, 40, 47, 49, 54, 58, 69, 77, 85, 93, 110,
113
<223> Methylated cytosine

<400> 12
tcgtgttttc gtttttttat cgtaggatgt tcggcggttc gggtatcgcg agtcggtcga 60
gttttagtcg gagttacgtg attacgttta ttcgtattta tagtttgggc gacg       114

<210> 13
<211> 16
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic primer

<400> 13
ggcggttcgg gtatcg                                                 16

<210> 14
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic primer

<400> 14
cgtaatcacg taactccgac t                                           21

<210> 15
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic flap probe

<220> 
<221> misc_difference
<222> 11, 16, 20
<223> Methylated cytosine

<220> 
<221> misc_difference
<222> 21
<223> 3'-C6 hexanediol modified

<400> 15
gacgcggagg cgagtcggtc g                                           21

<210> 16
<211> 100
<212> DNA
<213> Artificial Sequence

<220> 
<223> unmethylated vimentin fragment

<400> 16
ccgtgtcctc gtcctcctac cgcaggatgt tcggcggccc gggcaccgcg agccggccga 60
gctccagccg gagctacgtg actacgtcca cccgcaccta                       100

<210> 17
<211> 100
<212> DNA
<213> Artificial Sequence

<220> 
<223> Methylated vimentin fragment

<220> 
<221> misc_difference
<222> 2, 10, 21, 32, 35, 40, 47, 49, 54, 58, 69, 77, 85, 93
<223> Methylated cytosine

<400> 17
ccgtgtcctc gtcctcctac cgcaggatgt tcggcggccc gggcaccgcg agccggccga 60
gctccagccg gagctacgtg actacgtcca cccgcaccta                       100

<210> 18
<211> 100
<212> DNA
<213> Artificial Sequence

<220> 
<223> Unmethylated vimentin fragment after bisulfite
      reaction

<400> 18
uugtgtuutu gtuutuutau uguaggatgt tuggugguuu ggguauugug aguugguuga 60
gutuuaguug gagutaugtg autaugtuua uuuguauuta                       100

<210> 19
<211> 100
<212> DNA
<213> Artificial Sequence

<220> 
<223> Methylated vimentin fragment after bisulfite
      reaction

<220> 
<221> misc_difference
<222> 2, 10, 21, 32, 35, 40, 47, 49, 54, 58, 69, 77, 85, 93
<223> Methylated cytosine

<400> 19
ucgtgtuutc gtuutuutau cguaggatgt tcggcgguuc ggguaucgcg agucggucga 60
gutuuagucg gagutacgtg autacgtuua uucguauuta                       100
