                         SEQUENCE LISTING

<110>  Merck Serono SA
 
<120>  Antibody with specificity for GM-CSF (II)

<130>  48435P EP

<160>  44    

<170>  PatentIn version 3.3

<210>  1
<211>  5
<212>  PRT
<213>  Eutheria


<220>
<221>  MISC_FEATURE
<222>  (1)..(10)
<223>  4K21 heavy chain CDR1

<400>  1

Ala Tyr Asp Met Ser 
1               5   


<210>  2
<211>  17
<212>  PRT
<213>  Eutheria


<220>
<221>  MISC_FEATURE
<222>  (1)..(17)
<223>  4K21 heavy chain CDR2

<400>  2

Tyr Ile Ser Ser Gly Gly Ser Ser Phe Tyr Tyr Pro Asp Thr Val Lys 
1               5                   10                  15      


Gly 
    


<210>  3
<211>  6
<212>  PRT
<213>  Eutheria


<220>
<221>  MISC_FEATURE
<222>  (1)..(6)
<223>  4K21 heavy chain CDR3

<400>  3

His Leu Gly Phe Asp Tyr 
1               5       


<210>  4
<211>  16
<212>  PRT
<213>  Eutheria


<220>
<221>  MISC_FEATURE
<222>  (1)..(16)
<223>  4K21 light chain CDR1

<400>  4

Arg Ser Ser Gln Ser Leu Val Asn Ser Asn Gly Asn Thr Tyr Leu His 
1               5                   10                  15      


<210>  5
<211>  7
<212>  PRT
<213>  Eutheria


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  4K21 light chain CDR2

<400>  5

Lys Val Ser Asn Arg Phe Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  4K21 light chain CDR3 with H93Y mutation

<400>  6

Ser Gln Ser Thr Tyr Val Pro Pro Thr 
1               5                   


<210>  7
<211>  9
<212>  PRT
<213>  Eutheria


<220>
<221>  MISC_FEATURE
<222>  (1)..(9)
<223>  4K21 light chain CDR3

<400>  7

Ser Gln Ser Thr His Val Pro Pro Thr 
1               5                   


<210>  8
<211>  112
<212>  PRT
<213>  Artificial

<220>
<223>  humanized 4K21 variable region light chain with H93 mutation 
       (4K21 hVL-H93Y)

<400>  8

Asp Ile Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      


Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val Asn Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Ser 
                85                  90                  95      


Thr Tyr Val Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


<210>  9
<211>  112
<212>  PRT
<213>  Artificial

<220>
<223>  humanized 4K21 variable region light chain with mutations (4K21 
       hVL-S27eY-N28I-H34S-N53R-H93Y)

<400>  9

Asp Ile Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      


Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val Asn Tyr 
            20                  25                  30          


Ile Gly Asn Thr Tyr Leu Ser Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Lys Val Ser Arg Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Ser 
                85                  90                  95      


Thr Tyr Val Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


<210>  10
<211>  112
<212>  PRT
<213>  Artificial

<220>
<223>  humanized 4K21 variable region light chain with mutations (4K21 
       hVL-N28I-H34S-N53R-H93Y)

<400>  10

Asp Ile Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      


Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val Asn Ser 
            20                  25                  30          


Ile Gly Asn Thr Tyr Leu Ser Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Lys Val Ser Arg Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Ser 
                85                  90                  95      


Thr Tyr Val Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


<210>  11
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  4K21 heavy chain CDR2 with S55K & T62S mutations

<400>  11

Tyr Ile Ser Ser Gly Gly Lys Ser Phe Tyr Tyr Pro Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  12
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  4K21 heavy chain CDR2 with S55R & T62S mutations

<400>  12

Tyr Ile Ser Ser Gly Gly Arg Ser Phe Tyr Tyr Pro Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  13
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  4K21 heavy chain CDR2 with S56V & T62S mutations

<400>  13

Tyr Ile Ser Ser Gly Gly Ser Val Phe Tyr Tyr Pro Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  14
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  4K21 light chain CDR1 with N28I & H34S mutations

<400>  14

Arg Ser Ser Gln Ser Leu Val Asn Ser Ile Gly Asn Thr Tyr Leu Ser 
1               5                   10                  15      


<210>  15
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  4K21 light chain CDR1 with S27eY, N28I & H34S mutations

<400>  15

Arg Ser Ser Gln Ser Leu Val Asn Tyr Ile Gly Asn Thr Tyr Leu Ser 
1               5                   10                  15      


<210>  16
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  4K21 light chain CDR2 with N53R mutation

<400>  16

Lys Val Ser Arg Arg Phe Ser 
1               5           


<210>  17
<211>  115
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(115)
<223>  mouse 4K21 variable region heavy chain (4K21 mVH)

<400>  17

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Ser Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Ala Phe Ser Ala Tyr 
            20                  25                  30          


Asp Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Leu Val 
        35                  40                  45              


Ala Tyr Ile Ser Ser Gly Gly Ser Ser Phe Tyr Tyr Pro Asp Thr Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Lys Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Thr Arg His Leu Gly Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  18
<211>  115
<212>  PRT
<213>  Artificial

<220>
<223>  humanized 4K21 variable region heavy chain (4K21 hVH)

<400>  18

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ala Phe Ser Ala Tyr 
            20                  25                  30          


Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Arg Leu Glu Leu Val 
        35                  40                  45              


Ala Tyr Ile Ser Ser Gly Gly Ser Ser Phe Tyr Tyr Pro Asp Thr Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg His Leu Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  19
<211>  112
<212>  PRT
<213>  Mus musculus


<220>
<221>  MISC_FEATURE
<222>  (1)..(112)
<223>  mouse 4K21 variable region light chain (4K21 mVL)

<400>  19

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val Asn Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Phe Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  20
<211>  112
<212>  PRT
<213>  Artificial

<220>
<223>  humanized 4K21 variable region light chain (4K21 hVL)

<400>  20

Asp Ile Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      


Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val Asn Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Ser 
                85                  90                  95      


Thr His Val Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


<210>  21
<211>  115
<212>  PRT
<213>  Artificial

<220>
<223>  humanized 4K21 variable region heavy chain with mutations (4K21 
       hVH-S55K-T62S)

<400>  21

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ala Phe Ser Ala Tyr 
            20                  25                  30          


Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Arg Leu Glu Leu Val 
        35                  40                  45              


Ala Tyr Ile Ser Ser Gly Gly Lys Ser Phe Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg His Leu Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  22
<211>  115
<212>  PRT
<213>  Artificial

<220>
<223>  humanized 4K21 variable region heavy chain with mutations (4K21 
       hVH-S55R-T62S)

<400>  22

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ala Phe Ser Ala Tyr 
            20                  25                  30          


Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Arg Leu Glu Leu Val 
        35                  40                  45              


Ala Tyr Ile Ser Ser Gly Gly Arg Ser Phe Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg His Leu Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  23
<211>  115
<212>  PRT
<213>  Artificial

<220>
<223>  humanized 4K21 variable region heavy chain with mutations (4K21 
       hVH-S56V-T62S)

<400>  23

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ala Phe Ser Ala Tyr 
            20                  25                  30          


Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Arg Leu Glu Leu Val 
        35                  40                  45              


Ala Tyr Ile Ser Ser Gly Gly Ser Val Phe Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg His Leu Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  24
<211>  98
<212>  PRT
<213>  Artificial

<220>
<223>  IGHV3-7

<400>  24

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Asn Ile Lys Gln Asp Gly Ser Glu Lys Tyr Tyr Val Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg 
        


<210>  25
<211>  100
<212>  PRT
<213>  Artificial

<220>
<223>  IGKV2-28

<400>  25

Asp Ile Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      


Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu His Ser 
            20                  25                  30          


Asn Gly Tyr Asn Tyr Leu Asp Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Leu Gly Ser Asn Arg Ala Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln Ala 
                85                  90                  95      


Leu Gln Thr Pro 
            100 


<210>  26
<211>  144
<212>  PRT
<213>  Homo sapiens

<400>  26

Met Trp Leu Gln Ser Leu Leu Leu Leu Gly Thr Val Ala Cys Ser Ile 
1               5                   10                  15      


Ser Ala Pro Ala Arg Ser Pro Ser Pro Ser Thr Gln Pro Trp Glu His 
            20                  25                  30          


Val Asn Ala Ile Gln Glu Ala Arg Arg Leu Leu Asn Leu Ser Arg Asp 
        35                  40                  45              


Thr Ala Ala Glu Met Asn Glu Thr Val Glu Val Ile Ser Glu Met Phe 
    50                  55                  60                  


Asp Leu Gln Glu Pro Thr Cys Leu Gln Thr Arg Leu Glu Leu Tyr Lys 
65                  70                  75                  80  


Gln Gly Leu Arg Gly Ser Leu Thr Lys Leu Lys Gly Pro Leu Thr Met 
                85                  90                  95      


Met Ala Ser His Tyr Lys Gln His Cys Pro Pro Thr Pro Glu Thr Ser 
            100                 105                 110         


Cys Ala Thr Gln Ile Ile Thr Phe Glu Ser Phe Lys Glu Asn Leu Lys 
        115                 120                 125             


Asp Phe Leu Leu Val Ile Pro Phe Asp Cys Trp Glu Pro Val Gln Glu 
    130                 135                 140                 


<210>  27
<211>  144
<212>  PRT
<213>  Homo sapiens

<400>  27

Met Trp Leu Gln Ser Leu Leu Leu Leu Gly Thr Val Ala Cys Ser Ile 
1               5                   10                  15      


Ser Ala Pro Ala Arg Ser Pro Ser Pro Ser Thr Gln Pro Trp Glu His 
            20                  25                  30          


Val Asn Ala Ile Gln Glu Ala Arg Arg Leu Leu Asn Leu Ser Arg Asp 
        35                  40                  45              


Thr Ala Ala Glu Met Asn Glu Thr Val Glu Val Ile Ser Glu Met Phe 
    50                  55                  60                  


Asp Leu Gln Glu Pro Thr Cys Leu Gln Thr Arg Leu Glu Leu Tyr Lys 
65                  70                  75                  80  


Gln Gly Leu Arg Gly Ser Leu Thr Lys Leu Lys Gly Pro Leu Thr Met 
                85                  90                  95      


Met Ala Ser His Tyr Lys Gln His Cys Pro Pro Thr Pro Glu Thr Ser 
            100                 105                 110         


Cys Ala Thr Gln Thr Ile Thr Phe Glu Ser Phe Lys Glu Asn Leu Lys 
        115                 120                 125             


Asp Phe Leu Leu Val Ile Pro Phe Asp Cys Trp Glu Pro Val Gln Glu 
    130                 135                 140                 


<210>  28
<211>  144
<212>  PRT
<213>  Artificial

<220>
<223>  human GM-CSF with A117 mutation

<400>  28

Met Trp Leu Gln Ser Leu Leu Leu Leu Gly Thr Val Ala Cys Ser Ile 
1               5                   10                  15      


Ser Ala Pro Ala Arg Ser Pro Ser Pro Ser Thr Gln Pro Trp Glu His 
            20                  25                  30          


Val Asn Ala Ile Gln Glu Ala Arg Arg Leu Leu Asn Leu Ser Arg Asp 
        35                  40                  45              


Thr Ala Ala Glu Met Asn Glu Thr Val Glu Val Ile Ser Glu Met Phe 
    50                  55                  60                  


Asp Leu Gln Glu Pro Thr Cys Leu Gln Thr Arg Leu Glu Leu Tyr Lys 
65                  70                  75                  80  


Gln Gly Leu Arg Gly Ser Leu Thr Lys Leu Lys Gly Pro Leu Thr Met 
                85                  90                  95      


Met Ala Ser His Tyr Lys Gln His Cys Pro Pro Thr Pro Glu Thr Ser 
            100                 105                 110         


Cys Ala Thr Gln Ala Ile Thr Phe Glu Ser Phe Lys Glu Asn Leu Lys 
        115                 120                 125             


Asp Phe Leu Leu Val Ile Pro Phe Asp Cys Trp Glu Pro Val Gln Glu 
    130                 135                 140                 


<210>  29
<211>  144
<212>  PRT
<213>  Macaca mulatta

<400>  29

Met Trp Leu Gln Gly Leu Leu Leu Leu Gly Thr Val Ala Cys Ser Ile 
1               5                   10                  15      


Ser Ala Pro Ala Arg Ser Pro Ser Pro Gly Thr Gln Pro Trp Glu His 
            20                  25                  30          


Val Asn Ala Ile Gln Glu Ala Arg Arg Leu Leu Asn Leu Ser Arg Asp 
        35                  40                  45              


Thr Ala Ala Glu Met Asn Lys Thr Val Glu Val Val Ser Glu Met Phe 
    50                  55                  60                  


Asp Leu Gln Glu Pro Ser Cys Leu Gln Thr Arg Leu Glu Leu Tyr Lys 
65                  70                  75                  80  


Gln Gly Leu Gln Gly Ser Leu Thr Lys Leu Lys Gly Pro Leu Thr Met 
                85                  90                  95      


Met Ala Ser His Tyr Lys Gln His Cys Pro Pro Thr Pro Glu Thr Ser 
            100                 105                 110         


Cys Ala Thr Gln Ile Ile Thr Phe Gln Ser Phe Lys Glu Asn Leu Lys 
        115                 120                 125             


Asp Phe Leu Leu Val Ile Pro Phe Asp Cys Trp Glu Pro Val Gln Glu 
    130                 135                 140                 


<210>  30
<211>  144
<212>  PRT
<213>  Callithrix jacchus

<400>  30

Met Trp Leu Gln Asn Leu Leu Leu Leu Gly Thr Val Ala Gly Ser Ile 
1               5                   10                  15      


Ser Ala Pro Thr His Leu Pro Ser Pro Asp Thr Gln Pro Ser Lys His 
            20                  25                  30          


Val Asn Ala Ile Gln Glu Ala Gln Arg Leu Leu Asn Leu Ser Arg Asp 
        35                  40                  45              


Thr Ala Pro Glu Thr Asn Glu Thr Val Glu Val Val Ser Glu Met Phe 
    50                  55                  60                  


Asp Arg Gln Glu Pro Thr Cys Leu Gln Thr Arg Leu Glu Leu Tyr Lys 
65                  70                  75                  80  


Glu Ser Leu Trp Gly Ser Leu Thr Lys Leu Lys Gly Leu Leu Thr Met 
                85                  90                  95      


Ile Ala Ser His Tyr Lys Gln His Cys Pro Pro Thr Leu Glu Thr Ser 
            100                 105                 110         


Cys Ala Thr Lys Ile Ile Thr Phe Glu Ser Phe Lys Glu Asn Leu Lys 
        115                 120                 125             


Asp Phe Leu Leu Ala Ile Pro Val Asp Cys Trp Asp Pro Val Gln Glu 
    130                 135                 140                 


<210>  31
<211>  336
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encoding humanized 4K21 variable region light chain with H93Y
       mutation (4K21 hVL-H93Y)

<400>  31
gacatcgtga tgacccagtc ccccctgagc ctgcccgtga cccccggcga gcccgctagc     60

atcagctgcc ggtccagcca aagcttggtg aacagcaacg gcaacaccta cctgcactgg    120

tatctccaga agccaggaca gagcccccag ctgctgatct acaaggtctc caacaggttc    180

agcggcgtgc ccgaccggtt tagcggcagc ggctccggca ccgacttcac cctgaagatc    240

agccgggtgg aggccgagga cgtgggcgtg tactactgca gccagagcac ctacgtgccc    300

cccacctttg gccagggtac caaggtggag atcaag                              336


<210>  32
<211>  336
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encoding humanized 4K21 variable region light chain with 
       S27eY, N28I, H34S, N53R, H93Y mutations (4K21 
       hVL-S27eY-N28I-H34S-N53R-H93Y)

<400>  32
gacatcgtga tgacccagtc ccccctgagc ctgcccgtga cccccggcga gcccgctagc     60

atcagctgcc ggtccagcca aagcttggtg aactacatcg gcaacaccta cctgagctgg    120

tatctccaga agccaggaca gagcccccag ctgctgatct acaaggtctc ccgcaggttc    180

agcggcgtgc ccgaccggtt tagcggcagc ggctccggca ccgacttcac cctgaagatc    240

agccgggtgg aggccgagga cgtgggcgtg tactactgca gccagagcac ctacgtgccc    300

cccacctttg gccagggtac caaggtggag atcaag                              336


<210>  33
<211>  336
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encoding humanized 4K21 variable region light chain with 
       N28I, H34S, N53R, H93Y mutations (4K21 hVL-N28I-H34S-N53R-H93Y)

<400>  33
gacatcgtga tgacccagtc ccccctgagc ctgcccgtga cccccggcga gcccgctagc     60

atcagctgcc ggtccagcca aagcttggtg aacagcatcg gcaacaccta cctgagctgg    120

tatctccaga agccaggaca gagcccccag ctgctgatct acaaggtctc ccgcaggttc    180

agcggcgtgc ccgaccggtt tagcggcagc ggctccggca ccgacttcac cctgaagatc    240

agccgggtgg aggccgagga cgtgggcgtg tactactgca gccagagcac ctacgtgccc    300

cccacctttg gccagggtac caaggtggag atcaag                              336


<210>  34
<211>  345
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encoding murine 4K21 variable region heavy chain (4K21 mVH)

<400>  34
gaagtgcagc tggtggagtc tgggggaggc ttagtgaagt ctggagggtc cctgaaactc     60

tcctgtgcag cctctggatt cgctttcagt gcctatgaca tgtcttgggt tcgccagact    120

ccggagaaga ggctggaatt ggtcgcatac attagtagtg gtggtagtag tttttactat    180

ccagacactg tgaagggccg attcaccatc tccagagaca atgccaagaa caccctgtat    240

ctgcaaatga gcagtctgaa gtctgaggac acagccatgt attactgtac aagacatttg    300

gggtttgact actggggcca aggcaccact ctcacagtct cctca                    345


<210>  35
<211>  345
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encoding humanized 4K21 variable region heavy chain (4K21 
       hVH)

<400>  35
gaggtgcagc tggtcgagag cggcggaggg ctggtgcagc caggcggcag cttgaggctg     60

tcctgcgccg ctagcggatt tgctttcagc gcctacgaca tgagctgggt gcggcaggcc    120

cctggcaagc gtctagaact ggtggcctac atcagcagcg gcggcagcag cttctactac    180

cccgacaccg tgaagggccg gttcaccatc agccgggaca acgccaagaa cagcctgtac    240

ctgcagatga acagcctgcg ggccgaggac accgccgtgt actactgcac ccggcacctg    300

ggcttcgact actggggcca gggtaccctg gtgaccgtga gcagc                    345


<210>  36
<211>  336
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encoding murine 4K21 variable region light chain (4K21 mVL)

<400>  36
gacgtggtga tgacccagac ccccctgagc ctgcccgtga gcctgggcga ccaggccagc     60

atcagctgca ggagcagcca gagcctggtg aacagcaacg gcaacaccta cctgcactgg    120

ttcctgcaga agcccggcca gagccccaag ctgctgatct acaaggtgag caacaggttc    180

agcggcgtgc ccgacaggtt cagcggcagc ggcagcggca ccgacttcac cctgaagatc    240

agcagggtgg aggccgagga cctgggcgtg tacttctgca gccagagcac ccacgtgccc    300

cccaccttcg gcggcggtac caagctggag atcaag                              336


<210>  37
<211>  336
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encoding humanized 4K21 variable region light chain (4K21 
       hVL)

<400>  37
gacatcgtga tgacccagtc ccccctgagc ctgcccgtga cccccggcga gcccgctagc     60

atcagctgcc ggtccagcca aagcttggtg aacagcaacg gcaacaccta cctgcactgg    120

tatctccaga agccaggaca gagcccccag ctgctgatct acaaggtctc caacaggttc    180

agcggcgtgc ccgaccggtt tagcggcagc ggctccggca ccgacttcac cctgaagatc    240

agccgggtgg aggccgagga cgtgggcgtg tactactgca gccagagcac ccacgtgccc    300

cccacctttg gccagggtac caaggtggag atcaag                              336


<210>  38
<211>  345
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encoding humanized 4K21 variable region heavy chain with 
       S55K, T62S mutations (4K21 hVH-S55K-T62S)

<400>  38
gaggtgcagc tggtcgagag cggcggaggg ctggtgcagc caggcggcag cttgaggctg     60

tcctgcgccg ctagcggatt tgctttcagc gcctacgaca tgagctgggt gcggcaggcc    120

cctggcaagc gtctagaact ggtggcctac atcagcagcg gcggcaagag cttctactac    180

cccgacagcg tgaagggccg gttcaccatc agccgggaca acgccaagaa cagcctgtac    240

ctgcagatga acagcctgcg ggccgaggac accgccgtgt actactgcac ccggcacctg    300

ggcttcgact actggggcca gggtaccctg gtgaccgtga gcagc                    345


<210>  39
<211>  345
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encoding humanized 4K21 variable region heavy chain with 
       S55R, T62S mutations (4K21 hVH-S55R-T62S)

<400>  39
gaggtgcagc tggtcgagag cggcggaggg ctggtgcagc caggcggcag cttgaggctg     60

tcctgcgccg ctagcggatt tgctttcagc gcctacgaca tgagctgggt gcggcaggcc    120

cctggcaagc gtctagaact ggtggcctac atcagcagcg gcggccgcag cttctactac    180

cccgacagcg tgaagggccg gttcaccatc agccgggaca acgccaagaa cagcctgtac    240

ctgcagatga acagcctgcg ggccgaggac accgccgtgt actactgcac ccggcacctg    300

ggcttcgact actggggcca gggtaccctg gtgaccgtga gcagc                    345


<210>  40
<211>  345
<212>  DNA
<213>  Artificial

<220>
<223>  DNA encoding humanized 4K21 variable region heavy chain with 
       S56V, T62S mutations (4K21 hVH-S56V-T62S)

<400>  40
gaggtgcagc tggtcgagag cggcggaggg ctggtgcagc caggcggcag cttgaggctg     60

tcctgcgccg ctagcggatt tgctttcagc gcctacgaca tgagctgggt gcggcaggcc    120

cctggcaagc gtctagaact ggtggcctac atcagcagcg gcggcagcgt cttctactac    180

cccgacagcg tgaagggccg gttcaccatc agccgggaca acgccaagaa cagcctgtac    240

ctgcagatga acagcctgcg ggccgaggac accgccgtgt actactgcac ccggcacctg    300

ggcttcgact actggggcca gggtaccctg gtgaccgtga gcagc                    345


<210>  41
<211>  432
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(432)
<223>  human GM-CSF cDNA ORF with I117 SNP

<400>  41
atgtggctgc agagcctgct gctcttgggc actgtggcct gcagcatctc tgcacccgcc     60

cgctcgccca gccccagcac gcagccctgg gagcatgtga atgccatcca ggaggcccgg    120

cgtctcctga acctgagtag agacactgct gctgagatga atgaaacagt agaagtcatc    180

tcagaaatgt ttgacctcca ggagccgacc tgcctacaga cccgcctgga gctgtacaag    240

cagggcctgc ggggcagcct caccaagctc aagggcccct tgaccatgat ggccagccac    300

tacaagcagc actgccctcc aaccccggaa acttcctgtg caacccagat tatcaccttt    360

gaaagtttca aagagaacct gaaggacttt ctgcttgtca tcccctttga ctgctgggag    420

ccagtccagg ag                                                        432


<210>  42
<211>  432
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(432)
<223>  human GM-CSF cDNA ORF with T117 SNP

<400>  42
atgtggctgc agagcctgct gctcttgggc actgtggcct gcagcatctc tgcacccgcc     60

cgctcgccca gccccagcac gcagccctgg gagcatgtga atgccatcca ggaggcccgg    120

cgtctcctga acctgagtag agacactgct gctgagatga atgaaacagt agaagtcatc    180

tcagaaatgt ttgacctcca ggagccgacc tgcctacaga cccgcctgga gctgtacaag    240

cagggcctgc ggggcagcct caccaagctc aagggcccct tgaccatgat ggccagccac    300

tacaagcagc actgccctcc aaccccggaa acttcctgtg caacccagac tatcaccttt    360

gaaagtttca aagagaacct gaaggacttt ctgcttgtca tcccctttga ctgctgggag    420

ccagtccagg ag                                                        432


<210>  43
<211>  452
<212>  DNA
<213>  Macaca mulatta


<220>
<221>  misc_feature
<222>  (1)..(452)
<223>  rhesus/cynomolgus monkey GM-CSF cDNA ORF

<400>  43
aggctaaagt tctctggagg atgtggctgc agggcctgct gctcttgggc actgtggcct     60

gcagcatctc tgcacccgcc cgctcaccca gccccggcac gcagccctgg gagcatgtga    120

atgccatcca ggaggcccgg cgtctcctga acctgagtag agacactgct gctgagatga    180

ataaaaccgt agaagtcgtc tcagaaatgt ttgacctcca ggagccgagc tgcctacaga    240

cccgcctgga gctgtacaag cagggcctgc agggcagcct caccaagctc aagggcccct    300

tgaccatgat ggccagccac tacaagcagc actgccctcc aaccccggaa acttcctgtg    360

caacccagat tatcaccttc caaagtttca aagaaaacct gaaggacttt ctgcttgtca    420

tcccctttga ctgctgggag ccagtccagg ag                                  452


<210>  44
<211>  432
<212>  DNA
<213>  Callithrix jacchus


<220>
<221>  misc_feature
<222>  (1)..(432)
<223>  predicted marmoset GM-CSF cDNA

<400>  44
atgtggctgc agaacctgct gctcttgggc actgtggccg gcagcatctc tgcacctacc     60

cacttgccca gccccgacac acagccctcg aagcatgtga atgccatcca ggaggcccag    120

cgtctcctga acctgagtag agacactgct cctgagacaa atgaaacagt agaagtggtc    180

tcagaaatgt ttgaccgcca ggagccgacc tgcctacaga cccgcctgga gctgtacaag    240

gaaagcctgt ggggtagcct caccaagctc aagggactct tgaccatgat agccagccac    300

tacaagcagc actgcccccc aaccctggaa acttcctgtg caaccaagat tatcaccttt    360

gaaagtttca aagagaacct gaaggacttt ctgcttgcca tccctgttga ctgctgggat    420

ccagtccagg ag                                                        432


