                         SEQUENCE LISTING

<110>  Pieris AG
 
<120>  Muteins of human lipocalin 2 with affinity for Glypican-3 (GPC3)

<130>  PIE13917PCT

<150>  US 61/413,706
<151>  2010-11-15

<150>  EP 10 191 228.5
<151>  2010-11-15

<160>  27

<170>  PatentIn version 3.5

<210>  1
<211>  188
<212>  PRT
<213>  artificial

<220>
<223>  Mutein of hNGAL (G08)

<400>  1

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      

Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          

Val Val Gly Ile Ala Gly Asn Trp Ile Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              

Pro Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  

Asn Val Thr Arg Val Arg Phe Asp Glu Lys Lys Cys Gly Tyr Gly Ile 
65                  70                  75                  80  

Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Val 
                85                  90                  95      

Ile Lys Ser Arg Pro Gly Ile Thr Ser Asp Leu Val Arg Val Val Ser 
            100                 105                 110         

Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Lys Gln 
        115                 120                 125             

Asn Arg Glu Tyr Phe Gly Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 

Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 

Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     

Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            180                 185             
<210>  2
<211>  188
<212>  PRT
<213>  artificial

<220>
<223>  Mutein of hNGAL (A16)

<400>  2

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      

Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          

Val Val Gly Val Ala Gly Asn Ala Met Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              

Leu Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  

Asn Val Thr Gly Val Ser Phe Trp Arg Lys Lys Cys His Tyr Lys Ile 
65                  70                  75                  80  

Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 
                85                  90                  95      

Ile Lys Ser Gly Pro Gly Gln Thr Ser Asn Leu Val Arg Val Val Ser 
            100                 105                 110         

Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Arg Gln 
        115                 120                 125             

Asn Arg Glu Trp Phe Ala Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 

Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 

Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     

Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            180                 185             


<210>  3
<211>  188
<212>  PRT
<213>  artificial

<220>
<223>  Mutein of hNGAL (M02)

<400>  3

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      

Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          


Val Val Gly Arg Ala Gly Asn Val Ala Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              

Pro Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  

Asn Val Thr Asn Val Arg Phe Ala Met Lys Lys Cys Met Tyr Ser Ile 
65                  70                  75                  80  

Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gln 
                85                  90                  95      

Ile Lys Ser Glu Pro Gly Asn Thr Ser Asn Leu Val Arg Val Val Ser 
            100                 105                 110         

Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Glu Val Tyr Gln 
        115                 120                 125             

Asn Arg Glu Ile Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 

Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 

Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     

Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            180                 185             


<210>  4
<211>  188
<212>  PRT
<213>  artificial

<220>
<223>  Mutein of hNGAL (K21)

<400>  4

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      

Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          

Val Val Gly Met Ala Gly Asn Ala Arg Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              

Val Lys Met Thr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  

Asn Val Thr Ala Val Gln Phe Arg Leu Lys Lys Cys Gln Tyr Gly Ile 
65                  70                  75                  80  

Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 
                85                  90                  95      

Ile Lys Ser Ile Pro Gly Leu Thr Ser Met Leu Val Arg Val Val Ser 
            100                 105                 110         


Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Arg Val Arg Gln 
        115                 120                 125             

Asn Arg Glu Phe Phe Asp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 

Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 

Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     

Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            180                 185             


<210>  5
<211>  188
<212>  PRT
<213>  artificial

<220>
<223>  Mutein of hNGAL (O24)

<400>  5

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      

Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          

Val Val Gly Ser Ala Gly Asn His Arg Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              

Arg Lys Met His Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  

Asn Val Thr Asn Val Thr Phe Arg Glu Lys Lys Cys His Tyr Ser Ile 
65                  70                  75                  80  

Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Lys 
                85                  90                  95      

Ile Lys Ser Asn Pro Gly Met Thr Ser Phe Leu Val Arg Val Val Ser 
            100                 105                 110         

Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val His Gln 
        115                 120                 125             

Asn Arg Glu Gln Phe Asn Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 

Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 

Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     

Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            180                 185             


<210>  6
<211>  188
<212>  PRT
<213>  artificial

<220>
<223>  Mutein of hNGAL (O02)

<400>  6

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      

Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          

Val Val Gly Ile Ala Gly Asn Gly Gln Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              

Trp Lys Met Ser Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  

Asn Val Thr Ser Val Arg Phe Arg Leu Lys Lys Cys Ser Tyr Trp Ile 
65                  70                  75                  80  

Tyr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly 
                85                  90                  95      

Ile Lys Ser Asn Pro Gly Asp Thr Ser Asn Leu Val Arg Val Val Ser 
            100                 105                 110         

Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Tyr Val Ile Gln 
        115                 120                 125             

Asn Arg Glu Trp Phe Ile Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 

Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 

Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     

Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            180                 185             


<210>  7
<211>  188
<212>  PRT
<213>  artificial

<220>
<223>  Mutein of hNGAL (M04)

<400>  7

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      

Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          

Val Val Gly Met Ala Gly Asn Ala Ser Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              

Arg Lys Met Asn Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  

Asn Val Thr Lys Val Arg Phe Trp His Lys Lys Cys Tyr Tyr Ser Ile 
65                  70                  75                  80  

Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 
                85                  90                  95      

Ile Lys Ser Tyr Pro Gly Trp Thr Ser Tyr Leu Val Arg Val Val Ser 
            100                 105                 110         

Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Arg Gln 
        115                 120                 125             

Asn Arg Glu Trp Phe Ser Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 

Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 

Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     

Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            180                 185             


<210>  8
<211>  188
<212>  PRT
<213>  artificial

<220>
<223>  Mutein of hNGAL (O12)

<400>  8

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      

Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          

Val Val Gly Ile Ala Gly Asn Tyr Ile Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              

Pro Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  

Asn Val Thr Arg Val Phe Phe Ser Arg Lys Lys Cys Asp Tyr Ile Ile 
65                  70                  75                  80  

Trp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Phe 
                85                  90                  95      

Ile Lys Ser Asn Pro Gly Leu Thr Ser Leu Leu Val Arg Val Val Ser 
            100                 105                 110         

Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Trp Val Asp Gln 
        115                 120                 125             

Asn Arg Glu Val Phe Gly Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 

Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 

Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     

Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            180                 185             


<210>  9
<211>  3745
<212>  DNA
<213>  artificial

<220>
<223>  Expression vector phNGAL98 with AmpR encoding wild type Lcn2 with
       the C-terminal Strep-tagII

<400>  9
ccatcgaatg gccagatgat taattcctaa tttttgttga cactctatca ttgatagagt       60

tattttacca ctccctatca gtgatagaga aaagtgaaat gaatagttcg acaaaaatct      120

agataacgag ggcaaaaaat gaaaaagaca gctatcgcga ttgcagtggc tctggctggc      180

ttcgctaccg tagcgcaggc ccaggactcc acctcagacc tgatcccagc cccacctctg      240

agcaaggtcc ctctgcagca gaacttccag gacaaccaat tccatgggaa gtggtatgtg      300

gtaggtctcg cagggaatgc aattctcaga gaagacaaag acccgcaaaa gatgtatgcc      360

accatctatg agctgaaaga agacaagagc tacaatgtca cctccgtcct gtttaggaaa      420

aagaagtgtg actactggat caggactttt gttccaggtt cccagccagg cgagttcacg      480

ctgggcaaca ttaagagtta ccctggatta acgagttacc tcgtccgagt ggtgagcacc      540

aactacaacc agcatgctat ggtgttcttc aagaaagttt ctcaaaacag ggagtacttc      600

aagatcaccc tctacgggag aaccaaggag ctgacttcgg aactaaagga gaacttcatc      660

cgcttctcca aatctctggg cctccctgaa aaccacatcg tcttccctgt cccaatcgac      720

cagtgtatcg acggcagcgc ttggtctcac ccgcagttcg aaaaataata agcttgacct      780

gtgaagtgaa aaatggcgca cattgtgcga catttttttt gtctgccgtt taccgctact      840

gcgtcacgga tctccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta      900

cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc      960

cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt     1020

tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg     1080

gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca     1140

cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct     1200

attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga     1260

tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcaggtggca     1320

cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata     1380

tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga     1440

gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc     1500

ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg     1560

cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc     1620

ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat     1680

cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact     1740

tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat     1800

tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga     1860

tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc     1920

ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga     1980

tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag     2040

cttcccggca acaattgata gactggatgg aggcggataa agttgcagga ccacttctgc     2100

gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggct     2160

ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct     2220

acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg     2280

cctcactgat taagcattgg taggaattaa tgatgtctcg tttagataaa agtaaagtga     2340

ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac     2400

tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg     2460

ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt     2520

tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt     2580

tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt     2640

atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg     2700

cattatatgc actcagcgca gtggggcatt ttactttagg ttgcgtattg gaagatcaag     2760

agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat     2820

tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc     2880

ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tcttaaaagc     2940

agcataacct ttttccgtga tggtaacttc actagtttaa aaggatctag gtgaagatcc     3000

tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag     3060

accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct     3120

gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac     3180

caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc     3240

tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg     3300

ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt     3360

tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt     3420

gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc     3480

tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca     3540

gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata     3600

gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg     3660

ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct     3720

ggccttttgc tcacatgacc cgaca                                           3745


<210>  10
<211>  4746
<212>  DNA
<213>  artificial

<220>
<223>  Phage display vector phNGAL102 with CamR used as backbone for 
       NNK-Library

<400>  10
ccataacgct cggttgccgc cgggcgtttt ttattggcca gatgattaat tcctaatttt       60

tgttgacact ctatcattgg tagagttatt ttaccactcc ctatcagtga tagagaaaag      120

tgaaatgaat agttcgacaa aaatctagat aacgagggca aaaaatgaaa aagacagcta      180

tcgcgattgc agtggctctg gctggcttcg ctaccgtagc gcaggcccag gactccacct      240

cagacctgat cccagcccca cctctgagca aggtccctct gcagcagaac ttccaggaca      300

accaattcca tgggaagtgg tatgtggtag gtctcgcagg gaatgcaatt ctcagagaag      360

acaaagaccc gcaaaagatg tatgccacca tctatgagct gaaagaagac aagagctaca      420

atgtcacctc cgtcctgttt aggaaaaaga agtgtgacta ctggatcagg acttttgttc      480

caggttccca gccaggcgag ttcacgctgg gcaacattaa gagttaccct ggattaacga      540

gttacctcgt ccgagtggtg agcaccaact acaaccagca tgctatggtg ttcttcaaga      600

aagtttctca aaacagggag tacttcaaga tcaccctcta cgggagaacc aaggagctga      660

cttcggaact aaaggagaac ttcatccgct tctccaaatc tctgggcctc cctgaaaacc      720

acatcgtctt ccctgtccca atcgaccagt gtatcgacgg cagcgctggt ggggcctaga      780

ctgttgaaag ttgtttagca aaaccccata cagaaaattc atttactaac gtctggaaag      840

acgacaaaac tttagatcgt tacgctaact atgagggctg tctgtggaat gctacaggcg      900

ttgtagtttg tactggtgac gaaactcagt gttacggtac atgggttcct attgggcttg      960

ctatccctga aaatgagggt ggtggctctg agggtggcgg ttctgagggt ggcggttctg     1020

agggtggcgg tactaaacct cctgagtacg gtgatacacc tattccgggc tatacttata     1080

tcaaccctct cgacggcact tatccgcctg gtactgagca aaaccccgct aatcctaatc     1140

cttctcttga ggagtctcag cctcttaata ctttcatgtt tcagaataat aggttccgaa     1200

ataggcaggg ggcattaact gtttatacgg gcactgttac tcaaggcact gaccccgtta     1260

aaacttatta ccagtacact cctgtatcat caaaagccat gtatgacgct tactggaacg     1320

gtaaattcag agactgcgct ttccattctg gctttaatga ggatccattc gtttgtgaat     1380

atcaaggcca atcgtctgac ctgcctcaac ctcctgtcaa tgctggcggc ggctctggtg     1440

gtggttctgg tggcggctct gagggtggtg gctctgtggg tggcggttct gagggtggcg     1500

gctctgaggg aggcggttcc ggtggtggct ctggttccgg tgattttgat tatgaaaaga     1560

tggcaaacgc taataagggg gctatgaccg aaaatgccga tgaaaacgcg ctacagtctg     1620

acgctaaagg caaacttgat tctgtcgcta ctgattacgg tgctgctatc gatggtttca     1680

ttggtgacgt ttccggcctt gctaatggta atggtgctac tggtgatttt gctggctcta     1740

attcccaaat ggctcaagtc ggtgacggtg ataattcacc tttaatgaat aatttccgtc     1800

aatatttacc ttccctccct caatcggttg aatgtcgccc ttttgtcttt ggcgctggta     1860

aaccatatga attttctatt gattgtgaca aaataaactt attccgtggt gtctttgcgt     1920

ttcttttata tgttgccacc tttatgtatg tattttctac gtttgctaac atactgcgta     1980

ataaggagtc ttaataagct tgacctgtga agtgaaaaat ggcgcacatt gtgcgacatt     2040

ttttttgtct gccgtttacc gctactgcgt cacggatctc cacgcgccct gtagcggcgc     2100

attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct     2160

agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg     2220

tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga     2280

ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt     2340

ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg     2400

aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc     2460

ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat     2520

ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat     2580

aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa     2640

aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga     2700

acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga     2760

tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat     2820

tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg     2880

tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga     2940

aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata     3000

ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga     3060

gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt     3120

ggccaatatg gacaacttct tcgcccccgt tttcactatg ggcaaatatt atacgcaagg     3180

cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca     3240

tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta     3300

ataggaatta atgatgtctc gtttagataa aagtaaagtg attaacagcg cattagagct     3360

gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt     3420

agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc     3480

cattgagatg ttagataggc accatactca cttttgccct ttagaagggg aaagctggca     3540

agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg     3600

agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca     3660

attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc     3720

agtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga     3780

agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt     3840

atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg     3900

attagaaaaa caacttaaat gtgaaagtgg gtcttaaaag cagcataacc tttttccgtg     3960

atggtaactt cactagttta aaaggatcta ggtgaagatc ctttttgata atctcatgac     4020

caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa     4080

aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc     4140

accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt     4200

aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg     4260

ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc     4320

agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt     4380

accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga     4440

gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct     4500

tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg     4560

cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca     4620

cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa     4680

cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgac     4740

ccgaca                                                                4746


<210>  11
<211>  4963
<212>  DNA
<213>  artificial

<220>
<223>  Phage display vector phNGAL108 with AmpR used for Cloning Library

<400>  11
ccatcgaatg gccagatgat taattcctaa tttttgttga cactctatca ttgatagagt       60

tattttacca ctccctatca gtgatagaga aaagtgaaat gaatagttcg acaaaaatct      120

agataacgag ggcaaaaaat gaaaaagaca gctatcgcga ttgcagtggc tctggctggc      180

ttcgctaccg tagcgcaggc ccaggactcc acctcagacc tgatcccagc cccacctctg      240

agcaaggtcc ctctgcagca gaacttccag gacaaccaat tccatgggaa gtggtatgtg      300

gtaggtctcg cagggaatgc aattctcaga gaagacaaag acccgcaaaa gatgtatgcc      360

accatctatg agctgaaaga agacaagagc tacaatgtca cctccgtcct gtttaggaaa      420

aagaagtgtg actactggat caggactttt gttccaggtt cccagccagg cgagttcacg      480

ctgggcaaca ttaagagtta ccctggatta acgagttacc tcgtccgagt ggtgagcacc      540

aactacaacc agcatgctat ggtgttcttc aagaaagttt ctcaaaacag ggagtacttc      600

aagatcaccc tctacgggag aaccaaggag ctgacttcgg aactaaagga gaacttcatc      660

cgcttctcca aatctctggg cctccctgaa aaccacatcg tcttccctgt cccaatcgac      720

cagtgtatcg acggcagcgc ttggcgtcac ccgcagttcg gtggggccta gactgttgaa      780

agttgtttag caaaacccca tacagaaaat tcatttacta acgtctggaa agacgacaaa      840

actttagatc gttacgctaa ctatgagggc tgtctgtgga atgctacagg cgttgtagtt      900

tgtactggtg acgaaactca gtgttacggt acatgggttc ctattgggct tgctatccct      960

gaaaatgagg gtggtggctc tgagggtggc ggttctgagg gtggcggttc tgagggtggc     1020

ggtactaaac ctcctgagta cggtgataca cctattccgg gctatactta tatcaaccct     1080

ctcgacggca cttatccgcc tggtactgag caaaaccccg ctaatcctaa tccttctctt     1140

gaggagtctc agcctcttaa tactttcatg tttcagaata ataggttccg aaataggcag     1200

ggggcattaa ctgtttatac gggcactgtt actcaaggca ctgaccccgt taaaacttat     1260

taccagtaca ctcctgtatc atcaaaagcc atgtatgacg cttactggaa cggtaaattc     1320

agagactgcg ctttccattc tggctttaat gaggatccat tcgtttgtga atatcaaggc     1380

caatcgtctg acctgcctca acctcctgtc aatgctggcg gcggctctgg tggtggttct     1440

ggtggcggct ctgagggtgg tggctctgag ggtggcggtt ctgagggtgg cggctctgag     1500

ggaggcggtt ccggtggtgg ctctggttcc ggtgattttg attatgaaaa gatggcaaac     1560

gctaataagg gggctatgac cgaaaatgcc gatgaaaacg cgctacagtc tgacgctaaa     1620

ggcaaacttg attctgtcgc tactgattac ggtgctgcta tcgatggttt cattggtgac     1680

gtttccggcc ttgctaatgg taatggtgct actggtgatt ttgctggctc taattcccaa     1740

atggctcaag tcggtgacgg tgataattca cctttaatga ataatttccg tcaatattta     1800

ccttccctcc ctcaatcggt tgaatgtcgc ccttttgtct ttggcgctgg taaaccatat     1860

gaattttcta ttgattgtga caaaataaac ttattccgtg gtgtctttgc gtttctttta     1920

tatgttgcca cctttatgta tgtattttct acgtttgcta acatactgcg taataaggag     1980

tcttaataag cttgacctgt gaagtgaaaa atggcgcaca ttgtgcgaca ttttttttgt     2040

ctgccgttta ccgctactgc gtcacggatc tccacgcgcc ctgtagcggc gcattaagcg     2100

cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg     2160

ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc     2220

taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa     2280

aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc     2340

ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac     2400

tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt     2460

ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc     2520

ttacaatttc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt     2580

tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat     2640

aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt     2700

ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg     2760

ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga     2820

tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc     2880

tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac     2940

actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg     3000

gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca     3060

acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg     3120

gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg     3180

acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg     3240

gcgaactact tactctagct tcccggcaac aattgataga ctggatggag gcggataaag     3300

ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg     3360

gagccggtga gcgtggctct cgcggtatca ttgcagcact ggggccagat ggtaagccct     3420

cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac     3480

agatcgctga gataggtgcc tcactgatta agcattggta ggaattaatg atgtctcgtt     3540

tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag     3600

gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc     3660

atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc     3720

atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta     3780

aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac     3840

ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag     3900

gtttttcact agagaatgca ttatatgcac tcagcgcagt ggggcatttt actttaggtt     3960

gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg     4020

atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc     4080

cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg     4140

aaagtgggtc ttaaaagcag cataaccttt ttccgtgatg gtaacttcac tagtttaaaa     4200

ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt     4260

cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt     4320

ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt     4380

tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga     4440

taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag     4500

caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata     4560

agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg     4620

gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga     4680

gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca     4740

ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa     4800

acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt     4860

tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac     4920

ggttcctggc cttttgctgg ccttttgctc acatgacccg aca                       4963



<210>  12
<211>  197
<212>  PRT
<213>  Artificial

<220>
<223>  hNGAL with T7 tag encoded by phNGAL 101

<400>  12
Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      

Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          

Val Val Gly Leu Ala Gly Asn Ala Ile Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              

Gln Lys Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  

Asn Val Thr Ser Val Leu Phe Arg Lys Lys Lys Cys Asp Tyr Trp Ile 
65                  70                  75                  80  

Arg Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 
                85                  90                  95      

Ile Lys Ser Tyr Pro Gly Leu Thr Ser Tyr Leu Val Arg Val Val Ser 
            100                 105                 110         

Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Thr Val Ser Gln 
        115                 120                 125             

Asn Arg Glu Tyr Phe Lys Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 

Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 

Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     

Asp Gly Ser Ala Trp Ser His Pro Gln Phe Met Ala Ser Met Thr Gly 
            180                 185                 190         

Gly Gln Gln Met Gly 
        195


<210>  13
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  Primer (ASKD20)

<400>  13
CCACTCCCTA TCAGTGAT                                                  18


<210>  14
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  Primer (NGAL67)

<400>  14
CTTCACAGGT CAAGCTTATT ATTTTTCGAA C                                   31 


<210>  15
<211>  21
<212>  DNA
<213>  Artificial
<220>
<223>  Primer (3004-Slo1 bio)
<400>  15
CCAGGACAACCAATTCCATGGGAAATGGTATGTCGTGGGC                                30


<210>  16
<211>  88
<212>  DNA
<213>  Artificial

<220>
<223>  NNK oligomer for positions 36,  40, 41, 49, 52


<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (32)..(33)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (35)..(36)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (37)..(37)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (59)..(60)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (61)..(61)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (68)..(69)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (70)..(70)
<223>  k is g or t
<400>  16
gaagtggtat gtggtaggtn nkgcagggaa tnnknnkctc agagaagaca aagacccgnn     60

kaagatgnnk gccaccatct atgagctg                                        88


<210>  17
<211>  79
<212>  DNA
<213>  Artificial

<220>
<223>  NNK oligomer for positions 68, 70, 72, 73, 77, 79, 81

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (26)..(27)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (32)..(33)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (35)..(36)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (37)..(37)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (47)..(48)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(49)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (53)..(54)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (55)..(55)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (59)..(60)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (61)..(61)
<223>  k is g or t

<400>  17
caagagctac aatgtcaccn nkgtcnnktt tnnknnkaag aagtgtnnkt acnnkatcnn     60
kacttttgtt ccaggttcc                                                  79


<210>  18
<211>  68
<212>  DNA
<213>  Artificial

<220>
<223>  NNK oligomer for positions 96, 100, 103, 106

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (31)..(32)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (40)..(41)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (42)..(42)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (51)..(51)
<223>  k is g or t
<400>  18
ggcgagttca cgctgggcnn kattaagagt nnkcctggan nkacgagtnn kctcgtccga     60
gtggtgag                                                              68


<210>  19
<211>  68
<212>  DNA
<213>  Artificial

<220>
<223>  NNK oligomer for positions 125, 127, 132, 134

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (25)..(26)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (40)..(41)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (42)..(42)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (46)..(47)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (48)..(48)
<223>  k is g or t

<400>  19
gctatggtgt tcttcaagnn kgttnnkcaa aacagggagn nkttcnnkat caccctctac     60

gggagaac                                                              68


<210>  20
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  PCR-Primer with fixed nucleotide sequences corresponding to the 
       non-coding strand

<400>  20
ggtgacattg tagctcttgt cttctttcag ctcatagatg gtggc                     45


<210>  21
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  PCR-Primer with fixed nucleotide sequences corresponding to the 
       non-coding strand

<400>  21
gcccagcgtg aactcgcctg gctgggaacc tggaacaaaa gt                        42


<210>  22
<211>  54
<212>  DNA
<213>  Artificial

<220>
<223>  PCR-Primer with fixed nucleotide sequences corresponding to the 
       non-coding strand

<400>  22
cttgaagaac accatagcat gctggttgta gttggtgctc accactcgga cgag           54

<210>  23
<211>  64
<212>  DNA
<213>  Artificial

<220>
<223>  PCR-Primer with fixed nucleotide sequences corresponding to the 
       non-coding strand

<400>  23
ggagaagcgg atgaagttct cctttagttc cgaagtcagc tccttggttc tcccgtagag     60
ggtg                                                                  64


<210>  24
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  5' flanking PCR-Oligo biotinylated
<400>  24
ccaggacaac caattccatg ggaagtggta tgtggtaggt                           40


<210>  25
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  3' flanking PCR-Oligo biotinylated

<400>  25
ttcagggagg cccagagatt tggagaagcg gatgaagttc                           40


<210>  26
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  3' flanking PCR-Oligo biotinylated for Sloning Fragment

<400>  26
TTCAGGGAGG CCCAGAGATT TGGAAAAGCG GATAAAATTT                        40


<210>  27
<211>  178
<212>  PRT
<213>  Homo sapiens

<220>
<221>  MISC_FEATURE
<223>  wt Lcn2 (hNGAL)

<400>  27
Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      

Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          

Val Val Gly Leu Ala Gly Asn Ala Ile Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              

Gln Lys Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  

Asn Val Thr Ser Val Leu Phe Arg Lys Lys Lys Cys Asp Tyr Trp Ile 
65                  70                  75                  80  

Arg Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 
                85                  90                  95      

Ile Lys Ser Tyr Pro Gly Leu Thr Ser Tyr Leu Val Arg Val Val Ser 
            100                 105                 110         

Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Ser Gln 
        115                 120                 125             

Asn Arg Glu Tyr Phe Lys Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 

Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 

Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly 


