SEQUENCE LISTING

<110> Abbott Biotechnology Ltd. et al.

<120> Improved High Concentration Anti-TNF-alpha Antibody Liquid Formulation

<130> 117813-01020

<140> Concurrently filed herewith
<141> 2011-11-11

<150> US 61/412728
<151> 2010-11-11

<150> US 61/413960
<151> 2010-11-15

<160> 53

<170> PatentIn Ver. 2.0 


<210> 1
<211> 107
<212> PRT
<213> Artificial Sequence

<220>
<223> human antibody

<400> 1
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly
 1               5                  10                  15     
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Arg Asn Tyr
            20                  25                  30         
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
        35                  40                  45             
Tyr Ala Ala Ser Thr Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60                 
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65                  70                  75                  80 
Glu Asp Val Ala Thr Tyr Tyr Cys Gln Arg Tyr Asn Arg Ala Pro Tyr
                85                  90                  95     
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys
            100                 105        


<210> 2
<211> 121
<212> PRT
<213> Artificial Sequence

<220>

<223> human antibody

<400> 2
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg
 1               5                  10                  15     
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Asp Tyr
            20                  25                  30         
Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
        35                  40                  45             
Ser Ala Ile Thr Trp Asn Ser Gly His Ile Asp Tyr Ala Asp Ser Val
    50                  55                  60                 
Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr
65                  70                  75                  80 
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95     
Ala Lys Val Ser Tyr Leu Ser Thr Ala Ser Ser Leu Asp Tyr Trp Gly 
            100                 105                 110        
Gln Gly Thr Leu Val Thr Val Ser Ser
        115                 120    


<210> 3
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> human antibody

<220> 
<221> VARIANT
<222> (9)
<223> Xaa = Thr or Ala

<400> 3
Gln Arg Tyr Asn Arg Ala Pro Tyr Xaa
 1               5                 


<210> 4
<211> 12
<212> PRT
<213> Artificial Sequence

<220>
<223> human antibody

<220> 
<221> VARIANT
<222> (12)
<223> Xaa = Tyr or Asn

<400> 4
Val Ser Tyr Leu Ser Thr Ala Ser Ser Leu Asp Xaa
 1               5                  10         


<210> 5
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> human antibody

<400> 5
Ala Ala Ser Thr Leu Gln Ser
 1               5         


<210> 6
<211> 17
<212> PRT
<213> Artificial Sequence

<220> 
<223> human antibody

<400> 6
Ala Ile Thr Trp Asn Ser Gly His Ile Asp Tyr Ala Asp Ser Val Glu
 1               5                  10                  15     
Gly
   


<210> 7
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<223> human antibody

<400> 7
Arg Ala Ser Gln Gly Ile Arg Asn Tyr Leu Ala
 1               5                  10     


<210> 8
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> human antibody

<400> 8
Asp Tyr Ala Met His
 1               5 


<210> 9
<211> 107
<212> PRT
<213> Artificial Sequence

<220> 
<223> 2SD4 light chain variable region

<400> 9
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Ile Gly
 1               5                  10                  15     
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Arg Asn Tyr
            20                  25                  30         
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile
        35                  40                  45             
Tyr Ala Ala Ser Thr Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60                 
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
65                  70                  75                  80 
Glu Asp Val Ala Thr Tyr Tyr Cys Gln Lys Tyr Asn Ser Ala Pro Tyr
                85                  90                  95     
Ala Phe Gly Gln Gly Thr Lys Val Glu Ile Lys
            100                 105        


<210> 10
<211> 121
<212> PRT
<213> Artificial Sequence

<220> 
<223> 2SD4 heavy chain variable region

<400> 10
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg
 1               5                  10                  15     
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp Asp Tyr
            20                  25                  30         
Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Asp Trp Val
        35                  40                  45             
Ser Ala Ile Thr Trp Asn Ser Gly His Ile Asp Tyr Ala Asp Ser Val
    50                  55                  60                 
Glu Gly Arg Phe Ala Val Ser Arg Asp Asn Ala Lys Asn Ala Leu Tyr
65                  70                  75                  80 
Leu Gln Met Asn Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95     
Thr Lys Ala Ser Tyr Leu Ser Thr Ser Ser Ser Leu Asp Asn Trp Gly
            100                 105                 110        
Gln Gly Thr Leu Val Thr Val Ser Ser
        115                 120    


<210> 11
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> 2SD4 light chain variable region CDR3

<400> 11
Gln Lys Tyr Asn Ser Ala Pro Tyr Ala
 1               5                 


<210> 12
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> EP B12  light chain variable region CDR3

<400> 12
Gln Lys Tyr Asn Arg Ala Pro Tyr Ala
 1               5                 


<210> 13
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL10E4 light chain variable region CDR3

<400> 13
Gln Lys Tyr Gln Arg Ala Pro Tyr Thr
 1               5                 


<210> 14
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL100A9 light chain variable region CDR3


<400> 14
Gln Lys Tyr Ser Ser Ala Pro Tyr Thr
 1               5                 

<210> 15
<211> 9
<212> PRT
<213> Artificial Sequence


<220> 
<223> VLL100D2 light chain variable region CDR3

<400> 15
Gln Lys Tyr Asn Ser Ala Pro Tyr Thr
 1               5                 


<210> 16
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> VLL0F4 light chain variable region CDR3

<400> 16
Gln Lys Tyr Asn Arg Ala Pro Tyr Thr
 1               5                 


<210> 17
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> LOE5 light chain variable region CDR3

<400> 17
Gln Lys Tyr Asn Ser Ala Pro Tyr Tyr
 1               5                 


<210> 18
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> VLLOG7 light chain variable region CDR3

<400> 18
Gln Lys Tyr Asn Ser Ala Pro Tyr Asn
 1               5                 


<210> 19
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> VLLOG9 light chain variable region CDR3

<400> 19
Gln Lys Tyr Thr Ser Ala Pro Tyr Thr
 1               5                 


<210> 20
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> VLLOH1 light chain variable region CDR3

<400> 20
Gln Lys Tyr Asn Arg Ala Pro Tyr Asn
 1               5                 


<210> 21
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> VLLOH10 light chain variable region CDR3

<400> 21
Gln Lys Tyr Asn Ser Ala Ala Tyr Ser
 1               5                 


<210> 22
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL1B7 light chain variable region CDR3

<400> 22
Gln Gln Tyr Asn Ser Ala Pro Asp Thr
 1               5                 


<210> 23
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL1C1 light chain variable region CDR3

<400> 23
Gln Lys Tyr Asn Ser Asp Pro Tyr Thr
 1               5                 


<210> 24
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL0.1F4 light chain variable region CDR3

<400> 24
Gln Lys Tyr Ile Ser Ala Pro Tyr Thr
 1               5                 


<210> 25
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> VL0.1H8 light chain variable region CDR3

<400> 25
Gln Lys Tyr Asn Arg Pro Pro Tyr Thr
 1               5                 


<210> 26
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> LOE7.A light chain variable region CDR3

<400> 26
Gln Arg Tyr Asn Arg Ala Pro Tyr Ala
 1               5                 


<210> 27
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> 2SD4 heavy chain variable region CDR3

<400> 27
Ala Ser Tyr Leu Ser Thr Ser Ser Ser Leu Asp Asn
 1               5                  10         


<210> 28
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH1B11 heavy chain variable region CDR3

<400> 28
Ala Ser Tyr Leu Ser Thr Ser Ser Ser Leu Asp Lys
 1               5                  10         


<210> 29
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH1D8 heavy chain variable region CDR3

<400> 29
Ala Ser Tyr Leu Ser Thr Ser Ser Ser Leu Asp Tyr
 1               5                  10         


<210> 30
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH1A11 heavy chain variable region CDR3

<400> 30
Ala Ser Tyr Leu Ser Thr Ser Ser Ser Leu Asp Asp
 1               5                  10         


<210> 31
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> VH1B12 heavy chain variable region CDR3

<400> 31
Ala Ser Tyr Leu Ser Thr Ser Phe Ser Leu Asp Tyr
 1               5                  10         

<210> 32
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH1E4 heavy chain variable region CDR3

<400> 32
Ala Ser Tyr Leu Ser Thr Ser Ser Ser Leu His Tyr
 1               5                  10         


<210> 33
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH1F6 heavy chain variable region CDR3

<400> 33
Ala Ser Phe Leu Ser Thr Ser Ser Ser Leu Glu Tyr
 1               5                  10         


<210> 34
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> 3C-H2 heavy chain variable region CDR3

<400> 34
Ala Ser Tyr Leu Ser Thr Ala Ser Ser Leu Glu Tyr 
 1               5                  10          


<210> 35
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> VH1-D2.N heavy chain variable region CDR3

<400> 35
Val Ser Tyr Leu Ser Thr Ala Ser Ser Leu Asp Asn
 1               5                  10         


<210> 36
<211> 321
<212> DNA
<213> Artificial Sequence


<220> 
<223> adalimumab light chain variable region

<400> 36
gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtagggga cagagtcacc 60
atcacttgtc gggcaagtca gggcatcaga aattacttag cctggtatca gcaaaaacca 120
gggaaagccc ctaagctcct gatctatgct gcatccactt tgcaatcagg ggtcccatct 180
cggttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag cctacagcct 240
gaagatgttg caacttatta ctgtcaaagg tataaccgtg caccgtatac ttttggccag 300
gggaccaagg tggaaatcaa a                                           321


<210> 37
<211> 363
<212> DNA
<213> Artificial Sequence

<220> 
<223> adalimumab heavy chain variable region

<400> 37
gaggtgcagc tggtggagtc tgggggaggc ttggtacagc ccggcaggtc cctgagactc 60
tcctgtgcgg cctctggatt cacctttgat gattatgcca tgcactgggt ccggcaagct 120
ccagggaagg gcctggaatg ggtctcagct atcacttgga atagtggtca catagactat 180
gcggactctg tggagggccg attcaccatc tccagagaca acgccaagaa ctccctgtat 240
ctgcaaatga acagtctgag agctgaggat acggccgtat attactgtgc gaaagtctcg 300
taccttagca ccgcgtcctc ccttgactat tggggccaag gtaccctggt caccgtctcg 360
agt                                                               363


<210> 38
<211> 6
<212> PRT
<213> Homo sapiens

<400> 38
His Gly Ser His Asp Asn
  1               5    


<210> 39
<211> 12
<212> PRT
<213> Homo sapiens

<400> 39
Gln Ser Tyr Asp Arg Tyr Thr His Pro Ala Leu Leu
  1               5                  10        


<210> 40
<211> 17
<212> PRT
<213> Homo sapiens

<400> 40
Phe Ile Arg Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser Val Lys
  1               5                  10                  15    

Gly
   


<210> 41
<211> 7
<212> PRT
<213> Homo sapiens

<400> 41
Tyr Asn Asp Gln Arg Pro Ser
  1               5        


<210> 42
<211> 9
<212> PRT
<213> Homo sapiens

<400> 42 
Phe Thr Phe Ser Ser Tyr Gly Met His
  1               5                


<210> 43
<211> 13
<212> PRT
<213> Homo sapiens

<400> 43
Ser Gly Ser Arg Ser Asn Ile Gly Ser Asn Thr Val Lys
  1               5                  10            


<210> 44
<211> 115
<212> PRT
<213> Homo sapiens

<400> 44
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg
  1               5                  10                  15    

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30        

Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45            

Ala Phe Ile Arg Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser Val
     50                  55                  60                

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95    

Lys Thr His Gly Ser His Asp Asn Trp Gly Gln Gly Thr Met Val Thr
            100                 105                 110        

Val Ser Ser
        115


<210> 45
<211> 112
<212> PRT
<213> Homo sapiens

<400> 45
Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Gly Ala Pro Gly Gln
  1               5                  10                  15    

Arg Val Thr Ile Ser Cys Ser Gly Ser Arg Ser Asn Ile Gly Ser Asn
             20                  25                  30        

Thr Val Lys Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu
         35                  40                  45            

Ile Tyr Tyr Asn Asp Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser
     50                  55                  60                

Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Thr Gly Leu Gln
 65                  70                  75                  80

Ala Glu Asp Glu Ala Asp Tyr Tyr Cys Gln Ser Tyr Asp Arg Tyr Thr
                 85                  90                  95    

His Pro Ala Leu Leu Phe Gly Thr Gly Thr Lys Val Thr Val Leu Gly
            100                 105                 110        


<210> 46
<211> 16
<212> PRT
<213> Homo Sapiens

<400> 46
Ser Ile His Asn Arg Gly Thr Ile Phe Tyr Leu Asp Ser Val Lys Gly
 1               5                   10                 15     


<210> 47
<211> 10
<212> PRT
<213> Homo Sapiens

<400> 47
Gly Arg Ser Asn Ser Tyr Ala Met Asp Tyr
 1               5                  10 


<210> 48
<211> 16
<212> PRT
<213> Homo Sapiens

<400> 48
Arg Ser Thr Gln Thr Leu Val His Arg Asn Gly Asp Thr Tyr Leu Glu
 1               5                   10                 15     


<210> 49
<211> 7
<212> PRT
<213> Homo Sapiens

<400> 49
Lys Val Ser Asn Arg Phe Ser
 1               5         


<210> 50
<211> 9
<212> PRT
<213> Homo Sapiens

<400> 50
Phe Gln Gly Ser His Val Pro Tyr Thr
 1               5                 


<210> 51
<211> 30
<212> PRT
<213> Homo Sapiens

<400> 51
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val
 1               5                   10                 15                  20 

Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr
                25                   30


<210> 52
<211> 13
<212> PRT
<213> Homo Sapiens

<400> 52
Trp Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met Gly
 1               5                   10            


<210> 53
<211> 32
<212> PRT
<213> Homo Sapiens

<400> 53
Arg Val Thr Met Thr Arg Asp Thr Ser Thr Ser Thr Val Tyr Met Glu Leu Ser Ser Leu
 1               5                   10                 15                   20

Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg
                 25                  30        