
                                SEQUENCE LISTING

<110> Merck Sharp & Dohme Corp.
      McNeely, Tessie B.
      Cope, Leslie
      Smith, Sharon
      Joshi, Amita
      Pak, Irene
      Fridman, Arthur

<120> ENOLASE PEPTIDE CONJUGATE VACCINES
  AGAINST STAPHYLOCOCCUS AUREUS
  

<130> MRL-IFD-00028

<150> 61/412,998          
<151> 2010-11-12  

<160> 12

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> Enolase 213-230 peptide from S. aureus COL

<400> 1
Lys Phe Glu Gly Thr Glu Asp Ala Val Glu Thr Ile Ile Gln Ala Ile
 1               5                  10                  15      
Glu Ala
        


<210> 2
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> Enolase 419-434 peptide from S. aureus COL

<400> 2
Glu Thr Ala Lys Tyr Asp Gly Ile Lys Ser Phe Tyr Asn Leu Asp Lys
 1               5                  10                  15      


<210> 3
<211> 33
<212> PRT
<213> Artificial Sequence

<220> 
<223> His-tagged Enolase 213-230 peptide

<400> 3
Met Gly His His His His His His His His His His Lys Phe Glu Gly
 1               5                  10                  15      
Thr Glu Asp Ala Val Glu Thr Ile Ile Gln Ala Ile Glu Ala Ala Glu
            20                  25                  30          
Gln
    


<210> 4
<211> 31
<212> PRT
<213> Artificial Sequence

<220> 
<223> His-tagged Enolase 419-434 peptide

<400> 4
Met Gly His His His His His His His His His His Glu Thr Ala Lys
 1               5                  10                  15      
Tyr Asp Gly Ile Lys Ser Phe Tyr Asn Leu Asp Lys Ala Glu Gln
            20                  25                  30      


<210> 5
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Enolase 325-337 peptide from S. aureus COL

<400> 5
Thr Glu Ile Leu Ala Lys Gly Ile Glu Asn Gly Ile Gly
 1               5                  10              


<210> 6
<211> 33
<212> PRT
<213> Artificial Sequence

<220> 
<223> Enolase 252-284 peptide from S. aureus COL

<400> 6
Glu Asn Gly Val Tyr Asp Tyr Ser Lys Phe Glu Gly Glu His Gly Ala
 1               5                  10                  15      
Lys Arg Thr Ala Ala Glu Gln Val Asp Tyr Leu Glu Gln Leu Val Asp
            20                  25                  30          
Lys
    


<210> 7
<211> 645
<212> PRT
<213> S. aureus

<400> 7
Met Asn Lys Gln Gln Lys Glu Phe Lys Ser Phe Tyr Ser Ile Arg Lys
 1               5                  10                  15      
Ser Ser Leu Gly Val Ala Ser Val Ala Ile Ser Thr Leu Leu Leu Leu
            20                  25                  30          
Met Ser Asn Gly Glu Ala Gln Ala Ala Ala Glu Glu Thr Gly Gly Thr
        35                  40                  45              
Asn Thr Glu Ala Gln Pro Lys Thr Glu Ala Val Ala Ser Pro Thr Thr
    50                  55                  60                  
Thr Ser Glu Lys Ala Pro Glu Thr Lys Pro Val Ala Asn Ala Val Ser
65                  70                  75                  80  
Val Ser Asn Lys Glu Val Glu Ala Pro Thr Ser Glu Thr Lys Glu Ala
                85                  90                  95      
Lys Glu Val Lys Glu Val Lys Ala Pro Lys Glu Thr Lys Glu Val Lys
            100                 105                 110         
Pro Ala Ala Lys Ala Thr Asn Asn Thr Tyr Pro Ile Leu Asn Gln Glu
        115                 120                 125             
Leu Arg Glu Ala Ile Lys Asn Pro Ala Ile Lys Asp Lys Asp His Ser
    130                 135                 140                 
Ala Pro Asn Ser Arg Pro Ile Asp Phe Glu Met Lys Lys Lys Asp Gly
145                 150                 155                 160 
Thr Gln Gln Phe Tyr His Tyr Ala Ser Ser Val Lys Pro Ala Arg Val
                165                 170                 175     
Ile Phe Thr Asp Ser Lys Pro Glu Ile Glu Leu Gly Leu Gln Ser Gly
            180                 185                 190         
Gln Phe Trp Arg Lys Phe Glu Val Tyr Glu Gly Asp Lys Lys Leu Pro
        195                 200                 205             
Ile Lys Leu Val Ser Tyr Asp Thr Val Lys Asp Tyr Ala Tyr Ile Arg
    210                 215                 220                 
Phe Ser Val Ser Asn Gly Thr Lys Ala Val Lys Ile Val Ser Ser Thr
225                 230                 235                 240 
His Phe Asn Asn Lys Glu Glu Lys Tyr Asp Tyr Thr Leu Met Glu Phe
                245                 250                 255     
Ala Gln Pro Ile Tyr Asn Ser Ala Asp Lys Phe Lys Thr Glu Glu Asp
            260                 265                 270         
Tyr Lys Ala Glu Lys Leu Leu Ala Pro Tyr Lys Lys Ala Lys Thr Leu
        275                 280                 285             
Glu Arg Gln Val Tyr Glu Leu Asn Lys Ile Gln Asp Lys Leu Pro Glu
    290                 295                 300                 
Lys Leu Lys Ala Glu Tyr Lys Lys Lys Leu Glu Asp Thr Lys Lys Ala
305                 310                 315                 320 
Leu Asp Glu Gln Val Lys Ser Ala Ile Thr Glu Phe Gln Asn Val Gln
                325                 330                 335     
Pro Thr Asn Glu Lys Met Thr Asp Leu Gln Asp Thr Lys Tyr Val Val
            340                 345                 350         
Tyr Glu Ser Val Glu Asn Asn Glu Ser Met Met Asp Thr Phe Val Lys
        355                 360                 365             
His Pro Ile Lys Thr Gly Met Leu Asn Gly Lys Lys Tyr Met Val Met
    370                 375                 380                 
Glu Thr Thr Asn Asp Asp Tyr Trp Lys Asp Phe Met Val Glu Gly Gln
385                 390                 395                 400 
Arg Val Arg Thr Ile Ser Lys Asp Ala Lys Asn Asn Thr Arg Thr Ile
                405                 410                 415     
Ile Phe Pro Tyr Val Glu Gly Lys Thr Leu Tyr Asp Ala Ile Val Lys
            420                 425                 430         
Val His Val Lys Thr Ile Asp Tyr Asp Gly Gln Tyr His Val Arg Ile
        435                 440                 445             
Val Asp Lys Glu Ala Phe Thr Lys Ala Asn Thr Asp Lys Ser Asn Lys
    450                 455                 460                 
Lys Glu Gln Gln Asp Asn Ser Ala Lys Lys Glu Ala Thr Pro Ala Thr
465                 470                 475                 480 
Pro Ser Lys Pro Thr Pro Ser Pro Val Glu Lys Glu Ser Gln Lys Gln
                485                 490                 495     
Asp Ser Gln Lys Asp Asp Asn Lys Gln Leu Pro Ser Val Glu Lys Glu
            500                 505                 510         
Asn Asp Ala Ser Ser Glu Ser Gly Lys Asp Lys Thr Pro Ala Thr Lys
        515                 520                 525             
Pro Thr Lys Gly Glu Val Glu Ser Ser Ser Thr Thr Pro Thr Lys Val
    530                 535                 540                 
Val Ser Thr Thr Gln Asn Val Ala Lys Pro Thr Thr Ala Ser Ser Lys
545                 550                 555                 560 
Thr Thr Lys Asp Val Val Gln Thr Ser Ala Gly Ser Ser Glu Ala Lys
                565                 570                 575     
Asp Ser Ala Pro Leu Gln Lys Ala Asn Ile Lys Asn Thr Asn Asp Gly
            580                 585                 590         
His Thr Gln Ser Gln Asn Asn Lys Asn Thr Gln Glu Asn Lys Ala Lys
        595                 600                 605             
Ser Leu Pro Gln Thr Gly Glu Glu Ser Asn Lys Asp Met Thr Leu Pro
    610                 615                 620                 
Leu Met Ala Leu Leu Ala Leu Ser Ser Ile Val Ala Phe Val Leu Pro
625                 630                 635                 640 
Arg Lys Arg Lys Asn
                645 


<210> 8
<211> 434
<212> PRT
<213> S. aureus

<400> 8
Met Pro Ile Ile Thr Asp Val Tyr Ala Arg Glu Val Leu Asp Ser Arg
 1               5                  10                  15      
Gly Asn Pro Thr Val Glu Val Glu Val Leu Thr Glu Ser Gly Ala Phe
            20                  25                  30          
Gly Arg Ala Leu Val Pro Ser Gly Ala Ser Thr Gly Glu His Glu Ala
        35                  40                  45              
Val Glu Leu Arg Asp Gly Asp Lys Ser Arg Tyr Leu Gly Lys Gly Val
    50                  55                  60                  
Thr Lys Ala Val Glu Asn Val Asn Glu Ile Ile Ala Pro Glu Ile Ile
65                  70                  75                  80  
Glu Gly Glu Phe Ser Val Leu Asp Gln Val Ser Ile Asp Lys Met Met
                85                  90                  95      
Ile Ala Leu Asp Gly Thr Pro Asn Lys Gly Lys Leu Gly Ala Asn Ala
            100                 105                 110         
Ile Leu Gly Val Ser Ile Ala Val Ala Arg Ala Ala Ala Asp Leu Leu
        115                 120                 125             
Gly Gln Pro Leu Tyr Lys Tyr Leu Gly Gly Phe Asn Gly Lys Gln Leu
    130                 135                 140                 
Pro Val Pro Met Met Asn Ile Val Asn Gly Gly Ser His Ser Asp Ala
145                 150                 155                 160 
Pro Ile Ala Phe Gln Glu Phe Met Ile Leu Pro Val Gly Ala Thr Thr
                165                 170                 175     
Phe Lys Glu Ser Leu Arg Trp Gly Thr Glu Ile Phe His Asn Leu Lys
            180                 185                 190         
Ser Ile Leu Ser Lys Arg Gly Leu Glu Thr Ala Val Gly Asp Glu Gly
        195                 200                 205             
Gly Phe Ala Pro Lys Phe Glu Gly Thr Glu Asp Ala Val Glu Thr Ile
    210                 215                 220                 
Ile Gln Ala Ile Glu Ala Ala Gly Tyr Lys Pro Gly Glu Glu Val Phe
225                 230                 235                 240 
Leu Gly Phe Asp Cys Ala Ser Ser Glu Phe Tyr Glu Asn Gly Val Tyr
                245                 250                 255     
Asp Tyr Ser Lys Phe Glu Gly Glu His Gly Ala Lys Arg Thr Ala Ala
            260                 265                 270         
Glu Gln Val Asp Tyr Leu Glu Gln Leu Val Asp Lys Tyr Pro Ile Ile
        275                 280                 285             
Thr Ile Glu Asp Gly Met Asp Glu Asn Asp Trp Asp Gly Trp Lys Gln
    290                 295                 300                 
Leu Thr Glu Arg Ile Gly Asp Arg Val Gln Leu Val Gly Asp Asp Leu
305                 310                 315                 320 
Phe Val Thr Asn Thr Glu Ile Leu Ala Lys Gly Ile Glu Asn Gly Ile
                325                 330                 335     
Gly Asn Ser Ile Leu Ile Lys Val Asn Gln Ile Gly Thr Leu Thr Glu
            340                 345                 350         
Thr Phe Asp Ala Ile Glu Met Ala Gln Lys Ala Gly Tyr Thr Ala Val
        355                 360                 365             
Val Ser His Arg Ser Gly Glu Thr Glu Asp Thr Thr Ile Ala Asp Ile
    370                 375                 380                 
Ala Val Ala Thr Asn Ala Gly Gln Ile Lys Thr Gly Ser Leu Ser Arg
385                 390                 395                 400 
Thr Asp Arg Ile Ala Lys Tyr Asn Gln Leu Leu Arg Ile Glu Asp Glu
                405                 410                 415     
Leu Phe Glu Thr Ala Lys Tyr Asp Gly Ile Lys Ser Phe Tyr Asn Leu
            420                 425                 430         
Asp Lys
        


<210> 9
<211> 57
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence encoding Enolase 213-230
      peptide from S. aureus COL

<400> 9
aaatttgaag gtactgaaga tgctgttgaa acaattatcc aagcaatcga agcagct    57

<210> 10
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence encoding Enolase 419-434
      peptide from S. aureus COL

<400> 10
gaaactgcta aatatgacgg tatcaaatca ttctataact tagataaa              48

<210> 11
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence encoding Enolase 325-337
      peptide from S. aureus COL

<400> 11
actgaaattt tagcaaaagg tattgaaaac ggaattggt                        39

<210> 12
<211> 99
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleotide sequence encoding Enolase 252-284
      peptide from S. aureus COL

<400> 12
gaaaatggtg tatatgacta cagtaagttc gaaggcgaac acggtgcaaa acgtacagct 60
gcagaacaag ttgactactt agaacaatta gtagacaaa                        99
