
                                SEQUENCE LISTING

<110> Infectious Disease Research Institute
      Bhatia, Ajay
      Reed, Steven G.

<120> VACCINES COMPRISING NON-SPECIFIC
  NUCLEOSIDE HYDROLASE AND STEROL 24-C-METHYLTRANSFERASE (SMT)
  POLYPEPTIDES FOR THE TREATMENT AND DIAGNOSIS OF
  LEISHMANIASIS

<130> 480239.425PC	

<140> PCT/US                  
<141> 2011-11-07

<150> 61/411,366
<151> 2010-11-08  

<160> 14

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 314
<212> PRT
<213> Leishmania infantum

<400> 1
Met Pro Arg Lys Ile Ile Leu Asp Cys Asp Pro Gly Ile Asp Asp Ala
 1               5                  10                  15      
Val Ala Ile Phe Leu Ala His Gly Asn Pro Glu Val Glu Leu Leu Ala
            20                  25                  30          
Ile Thr Thr Val Val Gly Asn Gln Thr Leu Glu Lys Val Thr Arg Asn
        35                  40                  45              
Ala Arg Leu Val Ala Asp Val Ala Gly Ile Val Gly Val Pro Val Ala
    50                  55                  60                  
Ala Gly Cys Thr Lys Pro Leu Val Arg Gly Val Arg Asn Ala Ser Gln
65                  70                  75                  80  
Ile His Gly Glu Thr Gly Met Gly Asn Val Ser Tyr Pro Pro Glu Phe
                85                  90                  95      
Lys Thr Lys Leu Asp Gly Arg His Ala Val Gln Leu Ile Ile Asp Leu
            100                 105                 110         
Ile Met Ser His Glu Pro Lys Thr Ile Thr Leu Val Pro Thr Gly Gly
        115                 120                 125             
Leu Thr Asn Ile Ala Met Ala Val Arg Leu Glu Pro Arg Ile Val Asp
    130                 135                 140                 
Arg Val Lys Glu Val Val Leu Met Gly Gly Gly Tyr His Thr Gly Asn
145                 150                 155                 160 
Ala Ser Pro Val Ala Glu Phe Asn Val Phe Val Asp Pro Glu Ala Ala
                165                 170                 175     
His Ile Val Phe Asn Glu Ser Trp Asn Val Thr Met Val Gly Leu Asp
            180                 185                 190         
Leu Thr His Gln Ala Leu Ala Thr Pro Ala Val Gln Lys Arg Val Lys
        195                 200                 205             
Glu Val Gly Thr Lys Pro Ala Ala Phe Met Leu Gln Ile Leu Asp Phe
    210                 215                 220                 
Tyr Thr Lys Val Tyr Glu Lys Glu Arg Asn Thr Tyr Ala Thr Val His
225                 230                 235                 240 
Asp Pro Cys Ala Val Ala Tyr Val Ile Asp Pro Thr Val Met Thr Thr
                245                 250                 255     
Glu Gln Val Pro Val Asp Ile Glu Leu Asn Gly Ala Leu Thr Thr Gly
            260                 265                 270         
Met Thr Val Ala Asp Phe Arg Tyr Pro Arg Pro Lys His Cys His Thr
        275                 280                 285             
Gln Val Ala Val Lys Leu Asp Phe Asp Lys Phe Trp Cys Leu Val Ile
    290                 295                 300                 
Asp Ala Leu Lys Arg Ile Gly Asp Pro Gln
305                 310                 


<210> 2
<211> 942
<212> DNA
<213> Leishmania infantum

<400> 2
atgccgcgca agattattct cgattgtgat cccgggatcg atgatgccgt ggccatcttt 60
ctcgcccacg gcaacccgga ggtcgagctg ctggccatta cgacggtggt gggcaaccag 120
accctggaga aggtgacccg gaacgcgcgg ctggtagctg acgtagccgg catcgttggt 180
gtgcccgtcg cggctggttg caccaagccc ctcgtgcgcg gtgtgcggaa tgcctctcag 240
attcatggcg aaaccggcat gggtaacgtc tcctacccac cagagttcaa gacaaagttg 300
gacggccgcc atgcagtgca gctgatcatc gaccttatca tgtcgcacga gccgaagacg 360
atcacgcttg tgcctacggg tggcctgacg aacattgcga tggctgtccg tcttgagccg 420
cgcatcgtgg accgtgtgaa ggaggtggtt ctgatgggtg gcggctacca tactggtaat 480
gcgtcccctg tagcggagtt caacgtcttc gtcgacccgg aggcggcgca cattgtgttc 540
aacgagagct ggaacgtaac gatggtgggg ctggacctaa cgcaccaggc actcgccacg 600
ccggcggtcc agaagcgagt gaaggaggtg ggcacgaagc cggctgcctt catgctgcag 660
attttggact tttacacgaa ggtgtacgaa aaggagcgca acacgtacgc gacggtgcac 720
gatccctgcg ctgtggcgta cgtgattgac cccaccgtga tgacgacgga gcaagtgcca 780
gtggacatcg aactcaatgg ggcactgacg actgggatga cggtcgcgga cttccgctac 840
ccacggccaa agcactgcca cacgcaggtg gctgtgaagc tggacttcga caagttttgg 900
tgcctcgtga ttgacgcact caagcgcatc ggcgatcctc aa                    942

<210> 3
<211> 314
<212> PRT
<213> Leishmania donovani

<400> 3
Met Pro Arg Lys Ile Ile Leu Asp Cys Asp Pro Gly Ile Asp Asp Ala
 1               5                  10                  15      
Val Ala Ile Phe Leu Ala His Gly Asn Pro Glu Val Glu Leu Leu Ala
            20                  25                  30          
Ile Thr Thr Val Val Gly Asn Gln Thr Leu Glu Lys Val Thr Arg Asn
        35                  40                  45              
Ala Arg Leu Val Ala Asp Val Ala Gly Ile Val Gly Val Pro Val Ala
    50                  55                  60                  
Ala Gly Cys Thr Lys Pro Leu Val Arg Gly Val Arg Asn Ala Ser Gln
65                  70                  75                  80  
Ile His Gly Glu Thr Gly Met Gly Asn Val Ser Tyr Pro Pro Glu Phe
                85                  90                  95      
Lys Thr Lys Leu Asp Gly Arg His Ala Val Gln Leu Ile Ile Asp Leu
            100                 105                 110         
Ile Met Ser His Glu Pro Lys Thr Ile Thr Leu Val Pro Thr Gly Gly
        115                 120                 125             
Leu Thr Asn Ile Ala Met Ala Val Arg Leu Glu Pro Arg Ile Val Asp
    130                 135                 140                 
Arg Val Lys Glu Val Val Leu Met Gly Gly Gly Tyr His Thr Gly Asn
145                 150                 155                 160 
Ala Ser Pro Val Ala Glu Phe Asn Val Phe Val Asp Pro Glu Ala Ala
                165                 170                 175     
His Ile Val Phe Asn Glu Ser Trp Asn Val Thr Met Val Gly Leu Asp
            180                 185                 190         
Leu Thr His Gln Ala Leu Ala Thr Pro Ala Val Gln Lys Arg Val Lys
        195                 200                 205             
Glu Val Gly Thr Lys Pro Ala Ala Phe Met Leu Gln Ile Leu Asp Phe
    210                 215                 220                 
Tyr Thr Lys Val Tyr Glu Lys Glu Arg Asn Thr Tyr Ala Thr Val His
225                 230                 235                 240 
Asp Pro Cys Ala Val Ala Tyr Val Ile Asp Pro Thr Val Met Thr Thr
                245                 250                 255     
Glu Gln Val Pro Val Asp Ile Glu Leu Asn Gly Ala Leu Thr Thr Gly
            260                 265                 270         
Met Thr Val Ala Asp Phe Arg Tyr Pro Arg Pro Lys His Cys His Thr
        275                 280                 285             
Gln Val Ala Val Lys Leu Asp Phe Asp Lys Phe Trp Cys Leu Val Ile
    290                 295                 300                 
Asp Ala Leu Lys Arg Ile Gly Asp Pro Gln
305                 310                 


<210> 4
<211> 942
<212> DNA
<213> Leishmania donovani

<400> 4
atgccgcgca agattattct cgattgtgat cccgggatcg atgatgccgt ggccatcttt 60
ctcgcccacg gcaacccgga ggtcgagctg ctggccatta cgacggtggt gggcaaccag 120
accctggaga aggtgacccg gaacgcgcgg ctggtagctg acgtagccgg catcgttggt 180
gtgcccgtcg cggctggttg caccaagccc ctcgtgcgcg gtgtgcggaa tgcctctcag 240
attcatggcg aaaccggcat gggtaacgtc tcctacccac cagagttcaa gacaaagttg 300
gacggccgtc atgcagtgca gctgatcatc gaccttatca tgtcgcacga gccgaagacg 360
atcacgcttg tgcctacggg tggcctgacg aacattgcga tggctgtccg tcttgagccg 420
cgcatcgtgg accgtgtgaa ggaggtggtt ctgatgggtg gcggctacca tactggtaat 480
gcgtcccctg tagcggagtt caacgtcttc gtcgacccgg aggcggcgca cattgtgttc 540
aacgagagct ggaacgtaac gatggtgggg ctggacctaa cgcaccaggc actcgccacg 600
ccggcggtcc agaagcgagt gaaggaggtg ggcacgaagc cggctgcctt catgctgcag 660
attttggact tttacacgaa ggtgtacgaa aaggagcgca acacgtacgc gacggtgcac 720
gatccctgcg ctgtggcgta cgtgattgac cccaccgtga tgacgacgga gcaagtgcca 780
gtggacatcg agctcaatgg ggcactgacg actgggatga cggtcgcgga cttccgctac 840
ccacggccaa agcactgcca cacgcaggtg gctgtgaagc tggacttcga caagttttgg 900
tgcctcgtga ttgacgcact caagcgcatc ggcgatcctc aa                    942

<210> 5
<211> 314
<212> PRT
<213> Leishmania major

<400> 5
Met Pro Arg Lys Ile Ile Leu Asp Cys Asp Pro Gly Ile Asp Asp Ala
 1               5                  10                  15      
Val Ala Ile Phe Leu Ala His Gly Asn Pro Glu Ile Glu Leu Leu Ala
            20                  25                  30          
Ile Thr Thr Val Val Gly Asn Gln Ser Leu Glu Lys Val Thr Gln Asn
        35                  40                  45              
Ala Arg Leu Val Ala Asp Val Ala Gly Ile Val Gly Val Pro Val Ala
    50                  55                  60                  
Ala Gly Cys Thr Lys Pro Leu Val Arg Gly Val Arg Asn Ala Ser His
65                  70                  75                  80  
Ile His Gly Glu Thr Gly Met Gly Asn Val Ser Tyr Pro Pro Glu Phe
                85                  90                  95      
Lys Thr Lys Leu Asp Gly Arg His Ala Val Gln Leu Ile Ile Asp Leu
            100                 105                 110         
Ile Met Ser His Glu Pro Lys Thr Ile Thr Leu Val Pro Thr Gly Gly
        115                 120                 125             
Leu Thr Asn Ile Ala Met Ala Val Arg Leu Glu Pro Arg Ile Val Asp
    130                 135                 140                 
Arg Val Lys Glu Val Val Leu Met Gly Gly Gly Tyr His Thr Gly Asn
145                 150                 155                 160 
Ala Ser Pro Val Ala Glu Phe Asn Val Phe Ile Asp Pro Glu Ala Ala
                165                 170                 175     
His Ile Val Phe Asn Glu Ser Trp Asn Val Thr Met Val Gly Leu Asp
            180                 185                 190         
Leu Thr His Gln Ala Leu Ala Thr Pro Ala Val Gln Lys Arg Val Arg
        195                 200                 205             
Glu Val Gly Thr Lys Pro Ala Ala Phe Met Leu Gln Ile Leu Asp Phe
    210                 215                 220                 
Tyr Thr Lys Val Tyr Glu Lys Glu His Asp Thr Tyr Gly Lys Val His
225                 230                 235                 240 
Asp Pro Cys Ala Val Ala Tyr Val Ile Asp Pro Thr Val Met Thr Thr
                245                 250                 255     
Glu Arg Val Pro Val Asp Ile Glu Leu Asn Gly Ala Leu Thr Thr Gly
            260                 265                 270         
Met Thr Val Ala Asp Phe Arg Tyr Pro Arg Pro Lys Asn Cys Arg Thr
        275                 280                 285             
Gln Val Ala Val Lys Leu Asp Phe Asp Lys Phe Trp Cys Leu Val Ile
    290                 295                 300                 
Asp Ala Leu Glu Arg Ile Gly Asp Pro Gln
305                 310                 


<210> 6
<211> 942
<212> DNA
<213> Leishmania major

<400> 6
atgccgcgca agattattct cgactgtgat cccgggatcg atgatgccgt ggccatcttt 60
ctcgcccacg gcaacccgga gatcgagctg ctggccatta cgacggtggt gggcaaccag 120
agcctggaga aggtgaccca gaacgcgcgg ctggtagctg acgtagctgg catcgttggt 180
gtgcccgtcg cggctggctg caccaagccc ctcgtacgcg gtgtgcggaa tgcctctcat 240
attcacggcg aaaccggcat gggtaatgtc tcctacccac cagagttcaa gacaaagttg 300
gacggccgcc atgcagtgca gctgatcatc gaccttatca tgtcgcacga gcccaagacg 360
atcacgcttg tgcctacggg tggactgacg aacattgcga tggctgtccg tcttgagccg 420
cgcatcgtgg accgtgtgaa ggaggtggtt ctgatgggtg gcggctacca tactggtaat 480
gcttcccctg tcgcggagtt caacgtcttc atcgacccgg aggcggcgca cattgtgttc 540
aacgagagct ggaacgtaac aatggtggga ctggacctga cgcaccaggc cctcgccacg 600
ccggcggtcc agaagcgggt gagggaggtg ggcacgaagc cggctgcctt catgctgcag 660
attttggact tttacacgaa ggtgtacgaa aaggagcacg acacgtacgg gaaggtgcac 720
gatccgtgcg ctgtggcgta cgtgattgac cccaccgtga tgacgacgga gcgagtgcct 780
gtggacatcg agctcaatgg ggcactgacg actgggatga cggtcgcgga cttccgctac 840
ccgcggccaa agaactgccg cacgcaggtg gctgtgaagc tggacttcga caagttttgg 900
tgcctcgtga ttgacgcact cgagcgcatc ggcgatcctc ag                    942

<210> 7
<211> 353
<212> PRT
<213> Leishmania infantum

<400> 7
Met Ser Ala Gly Gly Arg Glu Thr Ala Pro Thr Asn Leu Ile Arg Arg
 1               5                  10                  15      
Arg Asn Lys Asp Glu Thr Asn Gly Asp Val Ser Ala Ala Ala Asp Arg
            20                  25                  30          
Phe Arg Asp Arg Phe Glu Lys Ala Thr Leu Glu Glu Arg Lys Ala Ala
        35                  40                  45              
Thr Thr Thr Met Val Asn Glu Tyr Tyr Asp Leu Val Thr Asp Phe Tyr
    50                  55                  60                  
Glu Tyr Gly Trp Gly Gln Asn Phe His Phe Ala Pro Arg Tyr Ala Gly
65                  70                  75                  80  
Glu Thr Phe Phe Glu Ser Leu Ala Arg His Glu Tyr Phe Leu Ala Ala
                85                  90                  95      
Arg Gly Gly Phe Met Glu Gly Asp His Ile Val Asp Val Gly Cys Gly
            100                 105                 110         
Val Gly Gly Pro Ala Arg Asn Met Val Arg Leu Thr Arg Cys Asn Val
        115                 120                 125             
Ile Gly Val Asn Asn Asn Asp Tyr Gln Ile Ser Arg Ala Arg Arg His
    130                 135                 140                 
Asp Ala Leu Ala Gly Met Ser Ser Lys Ile Asp Tyr Val Lys Thr Asp
145                 150                 155                 160 
Phe Cys Asn Met Ser Leu Ala Asp Asn Thr Phe Asp Gly Ala Tyr Ala
                165                 170                 175     
Ile Glu Ala Thr Cys His Ala Lys Asp Lys Val Lys Cys Tyr Ser Glu
            180                 185                 190         
Val Phe Arg Val Ile Lys Pro Gly Thr Cys Phe Val Leu Tyr Glu Trp
        195                 200                 205             
Cys Met Thr Asp Lys Tyr Asn Pro Asn Asp Glu Tyr His Arg Thr Ile
    210                 215                 220                 
Lys His Arg Ile Glu Leu Gly Asp Gly Leu Pro Glu Met Glu Thr Cys
225                 230                 235                 240 
Lys Gln Val Ile Glu Tyr Met Lys Gln Ala Gly Phe Val Val Glu Glu
                245                 250                 255     
Ala Ile Asp Val Ile Ser Gln Phe Glu Ser Ser Pro Ile Lys Ser Ile
            260                 265                 270         
Pro Trp Tyr Gln Pro Leu Val Gly Asp Tyr Ser Ser Leu Gln Gly Leu
        275                 280                 285             
Arg Ser Thr Pro Ile Gly Arg Ile Leu Thr Asn Val Met Cys Arg Val
    290                 295                 300                 
Leu Glu Phe Val Arg Leu Ala Pro Lys Gly Thr Tyr Lys Ala Thr Glu
305                 310                 315                 320 
Ile Leu Glu Glu Ala Ala Glu Ser Leu Val Val Gly Gly Gln Leu Gly
                325                 330                 335     
Ile Phe Thr Pro Ser Phe Tyr Ile Arg Ala Arg Lys Pro Ser Lys Gln
            340                 345                 350         
Ala
    


<210> 8
<211> 1059
<212> DNA
<213> Leishmania infantum

<400> 8
atgtccgccg gtggccgtga gaccgcgccg acgaacctga ttcgtcgccg caacaaggac 60
gagacaaacg gggatgtcag cgccgccgcc gaccgcttcc gcgaccgctt cgagaaggca 120
accctcgagg agcgcaaggc cgccaccacg acgatggtca acgagtacta cgacctggtg 180
acggacttct acgagtacgg ctggggccag aacttccatt tcgcgccgcg ctacgccggc 240
gagaccttct tcgagtccct cgcgcgccac gagtacttcc tggccgctcg cggcggcttc 300
atggagggcg accacatcgt cgacgtgggc tgcggcgtcg gcggtccggc gcgcaacatg 360
gttcgcctca cgcgctgcaa cgtcatcggc gtcaacaaca acgattacca gatcagccgc 420
gctcgccgtc atgacgcgct cgccggtatg agctccaaga tcgactacgt caagaccgac 480
ttctgcaaca tgagcttagc cgacaacacc ttcgacggcg cctacgccat cgaggccacc 540
tgccacgcaa aggacaaggt caagtgctat agcgaggtct tccgtgtcat caagcccggc 600
acctgctttg tcctgtacga gtggtgcatg accgacaagt acaaccccaa tgacgagtac 660
caccgcacaa tcaagcaccg catcgagctg ggcgacggcc tgccggagat ggagacgtgc 720
aaacaggtga tcgagtacat gaagcaggcc ggcttcgtgg tggaggaggc catagacgtc 780
atcagtcagt tcgagtccag ccccatcaag agtatcccgt ggtaccagcc gctggtcggc 840
gactattcgt ccctgcaggg cctgcgctct accccgattg gccgcatcct cacgaacgtc 900
atgtgtcgcg tgctggagtt cgtgcgccta gctccgaagg gcacgtacaa ggcgacggag 960
attttggagg aggctgcgga aagcctggtg gtgggcggtc agctcggcat cttcacgccg 1020
tccttctaca tccgcgctcg caagccgtcc aagcaggct                        1059

<210> 9
<211> 353
<212> PRT
<213> Leishmania donovani

<400> 9
Met Ser Ala Gly Gly Arg Glu Thr Ala Pro Thr Asn Leu Ile Arg Arg
 1               5                  10                  15      
Arg Asn Lys Asp Glu Thr Asn Gly Asp Val Ser Ala Ala Ala Asp Arg
            20                  25                  30          
Phe Arg Asp Arg Phe Glu Lys Ala Thr Leu Glu Glu Arg Lys Ala Ala
        35                  40                  45              
Thr Thr Thr Met Val Asn Glu Tyr Tyr Asp Leu Val Thr Asp Phe Tyr
    50                  55                  60                  
Glu Tyr Gly Trp Gly Gln Asn Phe His Phe Ala Pro Arg Tyr Ala Gly
65                  70                  75                  80  
Glu Thr Phe Phe Glu Ser Leu Ala Arg His Glu Tyr Phe Leu Ala Ala
                85                  90                  95      
Arg Gly Gly Phe Met Glu Gly Asp His Ile Val Asp Val Gly Cys Gly
            100                 105                 110         
Val Gly Gly Pro Ala Arg Asn Met Val Arg Leu Thr Arg Cys Asn Val
        115                 120                 125             
Ile Gly Val Asn Asn Asn Asp Tyr Gln Ile Ser Arg Ala Arg Arg His
    130                 135                 140                 
Asp Ala Leu Ala Gly Met Ser Ser Lys Ile Asp Tyr Val Lys Thr Asp
145                 150                 155                 160 
Phe Cys Asn Met Ser Leu Ala Asp Asn Thr Phe Asp Gly Ala Tyr Ala
                165                 170                 175     
Ile Glu Ala Thr Cys His Ala Lys Asp Lys Val Lys Cys Tyr Ser Glu
            180                 185                 190         
Val Phe Arg Val Ile Lys Pro Gly Thr Cys Phe Val Leu Tyr Glu Trp
        195                 200                 205             
Cys Met Thr Asp Lys Tyr Asn Pro Asn Asp Glu Tyr His Arg Thr Ile
    210                 215                 220                 
Lys His Arg Ile Glu Leu Gly Asp Gly Leu Pro Glu Met Glu Thr Cys
225                 230                 235                 240 
Lys Gln Val Ile Glu Tyr Met Lys Gln Ala Gly Phe Val Val Glu Glu
                245                 250                 255     
Ala Ile Asp Val Ile Ser Gln Phe Glu Ser Ser Pro Ile Lys Ser Ile
            260                 265                 270         
Pro Trp Tyr Gln Pro Leu Val Gly Asp Tyr Ser Ser Leu Gln Gly Leu
        275                 280                 285             
Arg Ser Thr Pro Ile Gly Arg Ile Leu Thr Asn Val Met Cys Arg Val
    290                 295                 300                 
Leu Glu Phe Val Arg Leu Ala Pro Lys Gly Thr Tyr Lys Ala Thr Glu
305                 310                 315                 320 
Val Leu Glu Glu Ala Ala Glu Ser Leu Val Val Gly Gly Gln Leu Gly
                325                 330                 335     
Ile Phe Thr Pro Ser Phe Tyr Ile Arg Ala Arg Lys Pro Ser Lys Gln
            340                 345                 350         
Ala
    


<210> 10
<211> 1059
<212> DNA
<213> Leishmania donovani

<400> 10
atgtccgccg gtggccgtga gaccgcgccg acgaacctga ttcgtcgccg caacaaggac 60
gagacaaacg gggatgtcag cgccgccgcc gaccgcttcc gcgaccgctt cgagaaggca 120
accctcgagg agcgcaaggc cgccaccacg acgatggtca acgagtacta cgacctggtg 180
acggacttct acgagtacgg ctggggccag aacttccatt tcgcgccgcg ctacgccggc 240
gagaccttct tcgagtccct cgcgcgccac gagtacttcc tggccgctcg cggcggcttc 300
atggagggcg accacatcgt cgacgtgggc tgcggcgtcg gcggtccggc gcgcaacatg 360
gttcgcctca cgcgctgcaa cgtcatcggc gtcaacaaca acgattacca gatcagccgc 420
gctcgccgtc atgacgcgct cgccggtatg agctccaaga tcgactacgt caagaccgac 480
ttctgcaaca tgagcttagc cgacaacacc ttcgacggcg cctacgccat cgaggccacc 540
tgccacgcaa aggacaaggt caagtgctat agcgaggtct tccgtgtcat caagcccggc 600
acctgctttg tcctgtacga gtggtgcatg accgacaagt acaaccccaa tgacgagtac 660
caccgcacaa tcaagcaccg catcgagctg ggcgacggcc tgccggagat ggagacgtgc 720
aaacaggtga tcgagtacat gaagcaggcc ggcttcgtgg tggaggaggc catagacgtc 780
atcagtcagt tcgaatccag ccccatcaag agtatcccgt ggtaccagcc gctggtcggc 840
gactattcgt ccctgcaggg cctgcgctct accccgattg gccgcatcct cacgaacgtc 900
atgtgtcgcg tgctggagtt cgtgcgccta gctccgaagg gcacgtacaa ggcgacggag 960
gttttggagg aggctgcgga aagcctggtg gtgggcggtc agctcggcat cttcacgccg 1020
tccttctaca tccgcgctcg caagccgtcc aagcaggct                        1059

<210> 11
<211> 353
<212> PRT
<213> Leishmania major

<400> 11
Met Ser Ala Gly Gly Arg Glu Thr Ala Pro Met Asn Leu Leu Arg Arg
 1               5                  10                  15      
Arg Asn Lys Asp Glu Ile Asn Gly Asp Val Asn Ala Ala Ala Asp Arg
            20                  25                  30          
Phe Arg Asn Arg Phe Glu Lys Ala Thr Leu Glu Glu Arg Lys Ala Ala
        35                  40                  45              
Thr Thr Thr Met Val Asn Glu Tyr Tyr Asp Leu Val Thr Asp Phe Tyr
    50                  55                  60                  
Glu Tyr Gly Trp Gly Gln Asn Phe His Phe Ala Pro Arg Tyr Ala Gly
65                  70                  75                  80  
Glu Thr Phe Phe Glu Ser Leu Ala Arg His Glu Tyr Phe Leu Ala Ala
                85                  90                  95      
Arg Gly Gly Phe Met Glu Gly Asp His Ile Val Asp Val Gly Cys Gly
            100                 105                 110         
Val Gly Gly Pro Ala Arg Asn Ile Val Arg Leu Thr Arg Cys Asn Val
        115                 120                 125             
Thr Gly Val Asn Asn Asn Asp Tyr Gln Ile Ser Arg Ala Arg Arg His
    130                 135                 140                 
Asp Ala Leu Ala Gly Met Ser Cys Lys Ile Asp Tyr Val Lys Thr Asp
145                 150                 155                 160 
Phe Cys Asn Met Ser Leu Ala Asp Asn Thr Phe Asp Gly Ala Tyr Ala
                165                 170                 175     
Ile Glu Ala Thr Cys His Ala Lys Asp Lys Val Lys Cys Tyr Ser Glu
            180                 185                 190         
Val Phe Arg Val Ile Lys Pro Gly Thr Cys Phe Val Leu Tyr Glu Trp
        195                 200                 205             
Cys Met Thr Asp Lys Tyr Asn Pro Asn Asp Glu Tyr His Arg Thr Ile
    210                 215                 220                 
Lys His Arg Ile Glu Leu Gly Asp Gly Leu Pro Glu Met Glu Thr Cys
225                 230                 235                 240 
Lys Gln Val Ile Glu Tyr Met Lys Glu Ala Gly Phe Val Val Glu Glu
                245                 250                 255     
Ala Ile Asp Val Ile Ser Gln Phe Glu Ser Ser Pro Ile Lys Ser Ile
            260                 265                 270         
Pro Trp Tyr Gln Pro Leu Val Gly Asp Tyr Ser Ser Leu Gln Gly Leu
        275                 280                 285             
Arg Ser Thr Pro Ile Gly Arg Ile Leu Thr Asn Ile Met Cys Arg Val
    290                 295                 300                 
Leu Glu Phe Val His Leu Ala Pro Lys Gly Thr Tyr Lys Ala Thr Glu
305                 310                 315                 320 
Val Leu Glu Glu Ala Ala Glu Ser Leu Val Val Gly Gly Gln Leu Gly
                325                 330                 335     
Ile Phe Thr Pro Ser Phe Tyr Ile Arg Ala Arg Lys Pro Ser Lys Gln
            340                 345                 350         
Ala
    


<210> 12
<211> 1059
<212> DNA
<213> Leishmania major

<400> 12
atgtctgccg gtggccgtga gaccgcgccg atgaacctgc ttcgtcgccg caacaaggat 60
gagataaacg gggatgtcaa cgccgccgcc gaccgcttcc gcaaccgctt cgagaaggca 120
accctcgagg agcgcaaggc cgccaccacg acgatggtca acgagtacta cgacctggtg 180
acggacttct acgagtacgg ctggggccag aactttcatt tcgcgccgcg ctacgccggc 240
gagaccttct tcgagtccct cgcgcgccac gagtacttcc tggccgcccg cggcggcttc 300
atggagggcg accatatcgt cgacgtgggc tgcggcgtcg gcggtccggc gcgcaacata 360
gttcgcctca cgcgctgtaa cgtcaccggc gtcaacaaca acgattacca aatcagccgc 420
gctcgccgtc atgacgcact cgccggtatg agctgcaaaa tcgactacgt caagaccgac 480
ttctgcaaca tgagcttagc cgacaacacc ttcgacggcg cctacgccat cgaggccaca 540
tgccacgcaa aggacaaggt caagtgctat agcgaggtct tccgtgtcat caagcccggc 600
acctgcttcg tcctgtacga gtggtgcatg accgacaagt acaaccccaa tgacgagtac 660
catcgcacga tcaagcaccg cattgagctg ggcgacggcc tgccggagat ggagacgtgc 720
aagcaggtga tcgagtacat gaaggaggcc ggtttcgtgg tggaggaagc catagatgtc 780
atcagtcagt tcgagtccag ccccatcaag agcatcccgt ggtaccagcc gctggttggc 840
gactactcgt ccctgcaggg cctgcgctct accccgattg gccgcatcct caccaacatc 900
atgtgtcgcg tgctggagtt cgtgcaccta gctccgaagg gcacgtacaa ggcgacggag 960
gttttggagg aggctgcgga aagcctggtg gtgggcggtc agctcggcat cttcacgccg 1020
tccttctaca tccgcgctcg caagccgtcc aagcaggcc                        1059

<210> 13
<211> 666
<212> PRT
<213> Artificial Sequence

<220> 
<223> NS fusion polypeptide

<400> 13
Met Pro Arg Lys Ile Ile Leu Asp Cys Asp Pro Gly Ile Asp Asp Ala
 1               5                  10                  15      
Val Ala Ile Phe Leu Ala His Gly Asn Pro Glu Val Glu Leu Leu Ala
            20                  25                  30          
Ile Thr Thr Val Val Gly Asn Gln Thr Leu Glu Lys Val Thr Arg Asn
        35                  40                  45              
Ala Arg Leu Val Ala Asp Val Ala Gly Ile Val Gly Val Pro Val Ala
    50                  55                  60                  
Ala Gly Cys Thr Lys Pro Leu Val Arg Gly Val Arg Asn Ala Ser Gln
65                  70                  75                  80  
Ile His Gly Glu Thr Gly Met Gly Asn Val Ser Tyr Pro Pro Glu Phe
                85                  90                  95      
Lys Thr Lys Leu Asp Gly Arg His Ala Val Gln Leu Ile Ile Asp Leu
            100                 105                 110         
Ile Met Ser His Glu Pro Lys Thr Ile Thr Leu Val Pro Thr Gly Gly
        115                 120                 125             
Leu Thr Asn Ile Ala Met Ala Val Arg Leu Glu Pro Arg Ile Val Asp
    130                 135                 140                 
Arg Val Lys Glu Val Val Leu Met Gly Gly Gly Tyr His Thr Gly Asn
145                 150                 155                 160 
Ala Ser Pro Val Ala Glu Phe Asn Val Phe Val Asp Pro Glu Ala Ala
                165                 170                 175     
His Ile Val Phe Asn Glu Ser Trp Asn Val Thr Met Val Gly Leu Asp
            180                 185                 190         
Leu Thr His Gln Ala Leu Ala Thr Pro Ala Val Gln Lys Arg Val Lys
        195                 200                 205             
Glu Val Gly Thr Lys Pro Ala Ala Phe Met Leu Gln Ile Leu Asp Phe
    210                 215                 220                 
Tyr Thr Lys Val Tyr Glu Lys Glu Arg Asn Thr Tyr Ala Thr Val His
225                 230                 235                 240 
Asp Pro Cys Ala Val Ala Tyr Val Ile Asp Pro Thr Val Met Thr Thr
                245                 250                 255     
Glu Gln Val Pro Val Asp Ile Glu Leu Asn Gly Ala Leu Thr Thr Gly
            260                 265                 270         
Met Thr Val Ala Asp Phe Arg Tyr Pro Arg Pro Lys His Cys His Thr
        275                 280                 285             
Gln Val Ala Val Lys Leu Asp Phe Asp Lys Phe Trp Cys Leu Val Ile
    290                 295                 300                 
Asp Ala Leu Lys Arg Ile Gly Asp Pro Gln Ser Ala Gly Gly Arg Glu
305                 310                 315                 320 
Thr Ala Pro Thr Asn Leu Ile Arg Arg Arg Asn Lys Asp Glu Thr Asn
                325                 330                 335     
Gly Asp Val Ser Ala Ala Ala Asp Arg Phe Arg Asp Arg Phe Glu Lys
            340                 345                 350         
Ala Thr Leu Glu Glu Arg Lys Ala Ala Thr Thr Thr Met Val Asn Glu
        355                 360                 365             
Tyr Tyr Asp Leu Val Thr Asp Phe Tyr Glu Tyr Gly Trp Gly Gln Asn
    370                 375                 380                 
Phe His Phe Ala Pro Arg Tyr Ala Gly Glu Thr Phe Phe Glu Ser Leu
385                 390                 395                 400 
Ala Arg His Glu Tyr Phe Leu Ala Ala Arg Gly Gly Phe Met Glu Gly
                405                 410                 415     
Asp His Ile Val Asp Val Gly Cys Gly Val Gly Gly Pro Ala Arg Asn
            420                 425                 430         
Met Val Arg Leu Thr Arg Cys Asn Val Ile Gly Val Asn Asn Asn Asp
        435                 440                 445             
Tyr Gln Ile Ser Arg Ala Arg Arg His Asp Ala Leu Ala Gly Met Ser
    450                 455                 460                 
Ser Lys Ile Asp Tyr Val Lys Thr Asp Phe Cys Asn Met Ser Leu Ala
465                 470                 475                 480 
Asp Asn Thr Phe Asp Gly Ala Tyr Ala Ile Glu Ala Thr Cys His Ala
                485                 490                 495     
Lys Asp Lys Val Lys Cys Tyr Ser Glu Val Phe Arg Val Ile Lys Pro
            500                 505                 510         
Gly Thr Cys Phe Val Leu Tyr Glu Trp Cys Met Thr Asp Lys Tyr Asn
        515                 520                 525             
Pro Asn Asp Glu Tyr His Arg Thr Ile Lys His Arg Ile Glu Leu Gly
    530                 535                 540                 
Asp Gly Leu Pro Glu Met Glu Thr Cys Lys Gln Val Ile Glu Tyr Met
545                 550                 555                 560 
Lys Gln Ala Gly Phe Val Val Glu Glu Ala Ile Asp Val Ile Ser Gln
                565                 570                 575     
Phe Glu Ser Ser Pro Ile Lys Ser Ile Pro Trp Tyr Gln Pro Leu Val
            580                 585                 590         
Gly Asp Tyr Ser Ser Leu Gln Gly Leu Arg Ser Thr Pro Ile Gly Arg
        595                 600                 605             
Ile Leu Thr Asn Val Met Cys Arg Val Leu Glu Phe Val Arg Leu Ala
    610                 615                 620                 
Pro Lys Gly Thr Tyr Lys Ala Thr Glu Ile Leu Glu Glu Ala Ala Glu
625                 630                 635                 640 
Ser Leu Val Val Gly Gly Gln Leu Gly Ile Phe Thr Pro Ser Phe Tyr
                645                 650                 655     
Ile Arg Ala Arg Lys Pro Ser Lys Gln Ala
            660                 665     


<210> 14
<211> 2001
<212> DNA
<213> Artificial Sequence

<220> 
<223> Nucleic acid sequence encoding NS fusion
      polypeptide

<400> 14
atgccgcgca agattattct cgattgtgat cccgggatcg atgatgccgt ggccatcttt 60
ctcgcccacg gcaacccgga ggtcgagctg ctggccatta cgacggtggt gggcaaccag 120
accctggaga aggtgacccg gaacgcgcgg ctggtagctg acgtagccgg catcgttggt 180
gtgcccgtcg cggctggttg caccaagccc ctcgtgcgcg gtgtgcggaa tgcctctcag 240
attcatggcg aaaccggcat gggtaacgtc tcctacccac cagagttcaa gacaaagttg 300
gacggccgtc atgcagtgca gctgatcatc gaccttatca tgtcgcacga gccgaagacg 360
atcacgcttg tgcctacggg tggcctgacg aacattgcga tggctgtccg tcttgagccg 420
cgcatcgtgg accgtgtgaa ggaggtggtt ctgatgggtg gcggctacca tactggtaat 480
gcgtcccctg tagcggagtt caacgtcttc gtcgacccgg aggcggcgca cattgtgttc 540
aacgagagct ggaacgtaac gatggtgggg ctggacctaa cgcaccaggc actcgccacg 600
ccggcggtcc agaagcgagt gaaggaggtg ggcacgaagc cggctgcctt catgctgcag 660
attttggact tttacacgaa ggtgtacgaa aaggagcgca acacgtacgc gacggtgcac 720
gatccctgcg ctgtggcgta cgtgattgac cccaccgtga tgacgacgga gcaagtgcca 780
gtggacatcg agctcaatgg ggcactgacg actgggatga cggtcgcgga cttccgctac 840
ccacggccaa agcactgcca cacgcaggtg gctgtgaagc tggacttcga caagttttgg 900
tgcctcgtga ttgacgcact caagcgcatc ggcgatcctc aatccgccgg tggccgtgag 960
accgcgccga cgaacctgat tcgtcgccgc aacaaggacg agacaaacgg ggatgtcagc 1020
gccgccgccg accgcttccg cgaccgcttc gagaaggcaa ccctcgagga gcgcaaggcc 1080
gccaccacga cgatggtcaa cgagtactac gacctggtga cggacttcta cgagtacggc 1140
tggggccaga acttccattt cgcgccgcgc tacgccggcg agaccttctt cgagtccctc 1200
gcgcgccacg agtacttcct ggccgctcgc ggcggcttca tggagggcga ccacatcgtc 1260
gacgtgggct gcggcgtcgg cggtccggcg cgcaacatgg ttcgcctcac gcgctgcaac 1320
gtcatcggcg tcaacaacaa cgattaccag atcagccgcg ctcgccgtca tgacgcgctc 1380
gccggtatga gctccaagat cgactacgtc aagaccgact tctgcaacat gagcttagcc 1440
gacaacacct tcgacggcgc ctacgccatc gaggccacct gccacgcaaa ggacaaggtc 1500
aagtgctata gcgaggtctt ccgtgtcatc aagcccggca cctgctttgt cctgtacgag 1560
tggtgcatga ccgacaagta caaccccaat gacgagtacc accgcacaat caagcaccgc 1620
atcgagctgg gcgacggcct gccggagatg gagacgtgca aacaggtgat cgagtacatg 1680
aagcaggccg gcttcgtggt ggaggaggcc atagacgtca tcagtcagtt cgagtccagc 1740
cccatcaaga gtatcccgtg gtaccagccg ctggtcggcg actattcgtc cctgcagggc 1800
ctgcgctcta ccccgattgg ccgcatcctc acgaacgtca tgtgtcgcgt gctggagttc 1860
gtgcgcctag ctccgaaggg cacgtacaag gcgacggaga ttttggagga ggctgcggaa 1920
agcctggtgg tgggcggtca gctcggcatc ttcacgccgt ccttctacat ccgcgctcgc 1980
aagccgtcca agcaggctta g                                           2001
