                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  Phospholipase C for degumming

<130>  11537

<160>  2     

<170>  PatentIn version 3.4

<210>  1
<211>  1932
<212>  DNA
<213>  Kinochaeta sp


<220>
<221>  CDS
<222>  (1)..(1929)

<220>
<221>  sig_peptide
<222>  (1)..(54)

<220>
<221>  mat_peptide
<222>  (55)..(1929)

<400>  1
atg cgt gcc tcc tcg att ctt tcg ctg gct ctg ggc ctc tcg gtt gcc       48
Met Arg Ala Ser Ser Ile Leu Ser Leu Ala Leu Gly Leu Ser Val Ala         
            -15                 -10                 -5                  

cag gcc gct gtg aac ccc gcc gat gtc ctg tct gtt gtg gag aag cga       96
Gln Ala Ala Val Asn Pro Ala Asp Val Leu Ser Val Val Glu Lys Arg         
    -1  1               5                   10                          

gtc gac ccg gct agc ggc cta gag gtg cgc agc att tgg gac acc atc      144
Val Asp Pro Ala Ser Gly Leu Glu Val Arg Ser Ile Trp Asp Thr Ile         
15                  20                  25                  30          

tgg aac gac att aaa tcg gcg gcc gac tgt act gcc tgc gag gcc gtc      192
Trp Asn Asp Ile Lys Ser Ala Ala Asp Cys Thr Ala Cys Glu Ala Val         
                35                  40                  45              

ttg act ctg ctc aag ggc gtc gcg gcc ttt ggc gat aat ttt ttc gta      240
Leu Thr Leu Leu Lys Gly Val Ala Ala Phe Gly Asp Asn Phe Phe Val         
            50                  55                  60                  

gag gtt ttg acc gag atc tgt gac ctt tcc ggg gct gag gat gat gat      288
Glu Val Leu Thr Glu Ile Cys Asp Leu Ser Gly Ala Glu Asp Asp Asp         
        65                  70                  75                      

gtg tgc tcc ggt gtt ctt agc ctc gag ggc cca atc ata gcc aac gat      336
Val Cys Ser Gly Val Leu Ser Leu Glu Gly Pro Ile Ile Ala Asn Asp         
    80                  85                  90                          

atc cgt aag atg agc att ggc tcc aag aca tca gag ctc ttc tgc atc      384
Ile Arg Lys Met Ser Ile Gly Ser Lys Thr Ser Glu Leu Phe Cys Ile         
95                  100                 105                 110         

acc ttc ctg gga ctg tgc tcg tac ccg gcg gtg gac gct ttc acc gtc      432
Thr Phe Leu Gly Leu Cys Ser Tyr Pro Ala Val Asp Ala Phe Thr Val         
                115                 120                 125             

ccc ttc ccg acc gcg aag tca gcc gcc acc cgg ccc gtg tcg tcg ggc      480
Pro Phe Pro Thr Ala Lys Ser Ala Ala Thr Arg Pro Val Ser Ser Gly         
            130                 135                 140                 

aaa gac ccc atc tac gtc gtg cac tac tct gac atc cac atc gat ccc      528
Lys Asp Pro Ile Tyr Val Val His Tyr Ser Asp Ile His Ile Asp Pro         
        145                 150                 155                     

ttc tat gtg gca gga tcc gcc agc aac tgc acc aag ccc atc tgc tgc      576
Phe Tyr Val Ala Gly Ser Ala Ser Asn Cys Thr Lys Pro Ile Cys Cys         
    160                 165                 170                         

cga gat tac act tcg gcg tcg tcc ccg ggc aac aac aac tcc cct gcc      624
Arg Asp Tyr Thr Ser Ala Ser Ser Pro Gly Asn Asn Asn Ser Pro Ala         
175                 180                 185                 190         

ggc ccg tac ggc gac cac aac tgc gac gtc ccg att agc ctg gag gac      672
Gly Pro Tyr Gly Asp His Asn Cys Asp Val Pro Ile Ser Leu Glu Asp         
                195                 200                 205             

agc atg tat gct gcc atc aag aag ctg gtg cct gat gcc gcc ttc ggc      720
Ser Met Tyr Ala Ala Ile Lys Lys Leu Val Pro Asp Ala Ala Phe Gly         
            210                 215                 220                 

atc ttt act ggc gat att gtc gac cac gcc gtc tgg aat acc tcg gag      768
Ile Phe Thr Gly Asp Ile Val Asp His Ala Val Trp Asn Thr Ser Glu         
        225                 230                 235                     

agt cag aac atc atc gac atg aat gac gcc tac acg cgc atg aag aac      816
Ser Gln Asn Ile Ile Asp Met Asn Asp Ala Tyr Thr Arg Met Lys Asn         
    240                 245                 250                         

tcg ggc atg ctg ccg acc atc ttc gcc acg gcg ggc aac cat gaa gcg      864
Ser Gly Met Leu Pro Thr Ile Phe Ala Thr Ala Gly Asn His Glu Ala         
255                 260                 265                 270         

tcg ccc gtc aac tcg ttc ccg ccg ccg gcc atc ggc aac gag tcg cag      912
Ser Pro Val Asn Ser Phe Pro Pro Pro Ala Ile Gly Asn Glu Ser Gln         
                275                 280                 285             

tgg gtt tac gac aca ctg gcc agc gac tgg agc cag tgg atc ggc acg      960
Trp Val Tyr Asp Thr Leu Ala Ser Asp Trp Ser Gln Trp Ile Gly Thr         
            290                 295                 300                 

tcg ggc gcg agc tcg gtc gag tcc atc ggc gct tac agc gtg cag tac     1008
Ser Gly Ala Ser Ser Val Glu Ser Ile Gly Ala Tyr Ser Val Gln Tyr         
        305                 310                 315                     

ggc agc acc aag ctg cgc gtc atc tcg ctc aac acc aac atg tac tac     1056
Gly Ser Thr Lys Leu Arg Val Ile Ser Leu Asn Thr Asn Met Tyr Tyr         
    320                 325                 330                         

atc gag aac ttc tac ctc tat gag ccc acc atg gag caa gat cca gcc     1104
Ile Glu Asn Phe Tyr Leu Tyr Glu Pro Thr Met Glu Gln Asp Pro Ala         
335                 340                 345                 350         

ggg cag ttc gcc tgg ctc gtg tcc gag ctg agc gcc gcc gaa gcc gcc     1152
Gly Gln Phe Ala Trp Leu Val Ser Glu Leu Ser Ala Ala Glu Ala Ala         
                355                 360                 365             

ggc gag cgc gtg tgg atc atc ggc cac atg ccg ctg ggt ctc tcg gac     1200
Gly Glu Arg Val Trp Ile Ile Gly His Met Pro Leu Gly Leu Ser Asp         
            370                 375                 380                 

gcc ttc cac gac ccg agc aac tac ttt gac cag atc gtc aac cgc tac     1248
Ala Phe His Asp Pro Ser Asn Tyr Phe Asp Gln Ile Val Asn Arg Tyr         
        385                 390                 395                     

gag gcc acc atc gcc gcc atg ttc ttc ggc cac acc cac gag gac cat     1296
Glu Ala Thr Ile Ala Ala Met Phe Phe Gly His Thr His Glu Asp His         
    400                 405                 410                         

ttc cag atc tcg tac tcg gac tac aac gcc cgc acg gcc gcc aac gcc     1344
Phe Gln Ile Ser Tyr Ser Asp Tyr Asn Ala Arg Thr Ala Ala Asn Ala         
415                 420                 425                 430         

cgc gcc gtc tcc tac atc atg ccg tcg ctg acg ccg acc tcg ggc cac     1392
Arg Ala Val Ser Tyr Ile Met Pro Ser Leu Thr Pro Thr Ser Gly His         
                435                 440                 445             

ccg acc ttc cgc gtc tac acg gtc gac ccc gag acc ttc ggc gtg ctg     1440
Pro Thr Phe Arg Val Tyr Thr Val Asp Pro Glu Thr Phe Gly Val Leu         
            450                 455                 460                 

gac gcg acg acc tac tac gcc gac atg tcg cag ccg acc tac cag acc     1488
Asp Ala Thr Thr Tyr Tyr Ala Asp Met Ser Gln Pro Thr Tyr Gln Thr         
        465                 470                 475                     

gcg ggg ccg gcc tgg tcc gtc tac tac agc gcc aag gcc gcc tac ggc     1536
Ala Gly Pro Ala Trp Ser Val Tyr Tyr Ser Ala Lys Ala Ala Tyr Gly         
    480                 485                 490                         

ggg ctc gtc gac ccg ccc gtc gcc gcc gac gac gcc gcc gcc gag ctg     1584
Gly Leu Val Asp Pro Pro Val Ala Ala Asp Asp Ala Ala Ala Glu Leu         
495                 500                 505                 510         

acg ccc gcc ttc tgg cac aac gtg acg gcc gcg ctg gcc gcc gac ccg     1632
Thr Pro Ala Phe Trp His Asn Val Thr Ala Ala Leu Ala Ala Asp Pro         
                515                 520                 525             

gcc agc ttc gac gcc tac tac gcg cgc aag acg cgc ggc tgg gac gtg     1680
Ala Ser Phe Asp Ala Tyr Tyr Ala Arg Lys Thr Arg Gly Trp Asp Val         
            530                 535                 540                 

gcc gcc tgc gcc ggc gcc tgc gcg gcc gcc gag gtc tgc gcc ctg cgc     1728
Ala Ala Cys Ala Gly Ala Cys Ala Ala Ala Glu Val Cys Ala Leu Arg         
        545                 550                 555                     

gcc gcc cgc gcc cag gac aac tgc gtc gtg ccc acg ccc ggc gtg cac     1776
Ala Ala Arg Ala Gln Asp Asn Cys Val Val Pro Thr Pro Gly Val His         
    560                 565                 570                         

ttc agc aag cgc gcc gac gag ggc acc ctg gcc cac cac cgc gac gag     1824
Phe Ser Lys Arg Ala Asp Glu Gly Thr Leu Ala His His Arg Asp Glu         
575                 580                 585                 590         

tgc ggc gtc agc gtc gcc cgc aac agc ctc tcc agc ctc gtc gtg cag     1872
Cys Gly Val Ser Val Ala Arg Asn Ser Leu Ser Ser Leu Val Val Gln         
                595                 600                 605             

cgc gag gcg ctg gag cac ctc gag ggc cgc ctg agc gag aag cgg agg     1920
Arg Glu Ala Leu Glu His Leu Glu Gly Arg Leu Ser Glu Lys Arg Arg         
            610                 615                 620                 

atg gcc gtg tga                                                     1932
Met Ala Val                                                             
        625                                                             


<210>  2
<211>  643
<212>  PRT
<213>  Kinochaeta sp

<400>  2

Met Arg Ala Ser Ser Ile Leu Ser Leu Ala Leu Gly Leu Ser Val Ala 
            -15                 -10                 -5          


Gln Ala Ala Val Asn Pro Ala Asp Val Leu Ser Val Val Glu Lys Arg 
    -1  1               5                   10                  


Val Asp Pro Ala Ser Gly Leu Glu Val Arg Ser Ile Trp Asp Thr Ile 
15                  20                  25                  30  


Trp Asn Asp Ile Lys Ser Ala Ala Asp Cys Thr Ala Cys Glu Ala Val 
                35                  40                  45      


Leu Thr Leu Leu Lys Gly Val Ala Ala Phe Gly Asp Asn Phe Phe Val 
            50                  55                  60          


Glu Val Leu Thr Glu Ile Cys Asp Leu Ser Gly Ala Glu Asp Asp Asp 
        65                  70                  75              


Val Cys Ser Gly Val Leu Ser Leu Glu Gly Pro Ile Ile Ala Asn Asp 
    80                  85                  90                  


Ile Arg Lys Met Ser Ile Gly Ser Lys Thr Ser Glu Leu Phe Cys Ile 
95                  100                 105                 110 


Thr Phe Leu Gly Leu Cys Ser Tyr Pro Ala Val Asp Ala Phe Thr Val 
                115                 120                 125     


Pro Phe Pro Thr Ala Lys Ser Ala Ala Thr Arg Pro Val Ser Ser Gly 
            130                 135                 140         


Lys Asp Pro Ile Tyr Val Val His Tyr Ser Asp Ile His Ile Asp Pro 
        145                 150                 155             


Phe Tyr Val Ala Gly Ser Ala Ser Asn Cys Thr Lys Pro Ile Cys Cys 
    160                 165                 170                 


Arg Asp Tyr Thr Ser Ala Ser Ser Pro Gly Asn Asn Asn Ser Pro Ala 
175                 180                 185                 190 


Gly Pro Tyr Gly Asp His Asn Cys Asp Val Pro Ile Ser Leu Glu Asp 
                195                 200                 205     


Ser Met Tyr Ala Ala Ile Lys Lys Leu Val Pro Asp Ala Ala Phe Gly 
            210                 215                 220         


Ile Phe Thr Gly Asp Ile Val Asp His Ala Val Trp Asn Thr Ser Glu 
        225                 230                 235             


Ser Gln Asn Ile Ile Asp Met Asn Asp Ala Tyr Thr Arg Met Lys Asn 
    240                 245                 250                 


Ser Gly Met Leu Pro Thr Ile Phe Ala Thr Ala Gly Asn His Glu Ala 
255                 260                 265                 270 


Ser Pro Val Asn Ser Phe Pro Pro Pro Ala Ile Gly Asn Glu Ser Gln 
                275                 280                 285     


Trp Val Tyr Asp Thr Leu Ala Ser Asp Trp Ser Gln Trp Ile Gly Thr 
            290                 295                 300         


Ser Gly Ala Ser Ser Val Glu Ser Ile Gly Ala Tyr Ser Val Gln Tyr 
        305                 310                 315             


Gly Ser Thr Lys Leu Arg Val Ile Ser Leu Asn Thr Asn Met Tyr Tyr 
    320                 325                 330                 


Ile Glu Asn Phe Tyr Leu Tyr Glu Pro Thr Met Glu Gln Asp Pro Ala 
335                 340                 345                 350 


Gly Gln Phe Ala Trp Leu Val Ser Glu Leu Ser Ala Ala Glu Ala Ala 
                355                 360                 365     


Gly Glu Arg Val Trp Ile Ile Gly His Met Pro Leu Gly Leu Ser Asp 
            370                 375                 380         


Ala Phe His Asp Pro Ser Asn Tyr Phe Asp Gln Ile Val Asn Arg Tyr 
        385                 390                 395             


Glu Ala Thr Ile Ala Ala Met Phe Phe Gly His Thr His Glu Asp His 
    400                 405                 410                 


Phe Gln Ile Ser Tyr Ser Asp Tyr Asn Ala Arg Thr Ala Ala Asn Ala 
415                 420                 425                 430 


Arg Ala Val Ser Tyr Ile Met Pro Ser Leu Thr Pro Thr Ser Gly His 
                435                 440                 445     


Pro Thr Phe Arg Val Tyr Thr Val Asp Pro Glu Thr Phe Gly Val Leu 
            450                 455                 460         


Asp Ala Thr Thr Tyr Tyr Ala Asp Met Ser Gln Pro Thr Tyr Gln Thr 
        465                 470                 475             


Ala Gly Pro Ala Trp Ser Val Tyr Tyr Ser Ala Lys Ala Ala Tyr Gly 
    480                 485                 490                 


Gly Leu Val Asp Pro Pro Val Ala Ala Asp Asp Ala Ala Ala Glu Leu 
495                 500                 505                 510 


Thr Pro Ala Phe Trp His Asn Val Thr Ala Ala Leu Ala Ala Asp Pro 
                515                 520                 525     


Ala Ser Phe Asp Ala Tyr Tyr Ala Arg Lys Thr Arg Gly Trp Asp Val 
            530                 535                 540         


Ala Ala Cys Ala Gly Ala Cys Ala Ala Ala Glu Val Cys Ala Leu Arg 
        545                 550                 555             


Ala Ala Arg Ala Gln Asp Asn Cys Val Val Pro Thr Pro Gly Val His 
    560                 565                 570                 


Phe Ser Lys Arg Ala Asp Glu Gly Thr Leu Ala His His Arg Asp Glu 
575                 580                 585                 590 


Cys Gly Val Ser Val Ala Arg Asn Ser Leu Ser Ser Leu Val Val Gln 
                595                 600                 605     


Arg Glu Ala Leu Glu His Leu Glu Gly Arg Leu Ser Glu Lys Arg Arg 
            610                 615                 620         


Met Ala Val 
        625 


