                         SEQUENCE LISTING

<110>  Deinove
 
<120>  LACCASES AND USES THEREOF

<130>  B1085PC00

<160>  9     

<170>  PatentIn version 3.3

<210>  1
<211>  1446
<212>  DNA
<213>  Deinococcus sp.


<220>
<221>  misc_feature
<223>  Laccase 1 derived from Deinococcus cellulolyticus DRH46

<400>  1
atgaaacaga ggctcactgc agcagccctg ctgactgccc tgttgtccag ttgtgccatg     60

aaaaacccgg atgtcatttc gtatcaggac cctgagccac tggcaggcca gcgcagtggc    120

aaagctgccg aattcaccct caccgcgcag aaaagccgtc tggaccttgc aggccagtcg    180

gtggaggccc tgacctacaa cggcacgttc ccgggtcctc tgctgaccct gaaagccgga    240

gaggacgtgc ggatcaccct caaaaacaac ctctccgagc ccaccaacct gcacctgcac    300

ggactccccc tctctccaga ggtggatgat ccttttgtgg tggtccctcc cgggggatct    360

cacacctacg ctttccaggt gcctgaagag gtgcatggca ccttctggta tcacacccac    420

acccacgggg cttctgcagc acaactgttt tcaggcctgg caggaccact gttggtggag    480

gcagcaaagc cgaacaaccc cattgagcac atggagaccc acacggtggt cttcaaggac    540

ctgccaggtg cgcagcacgc agacgacggt gtcctgatga acggacgtga aaacaccctg    600

ctggtcaatg gccttgtctc tcccactttg aagaccgaaa acagctgggt gcagctccgc    660

ctgatcaatg cctccaatgc ccgttacctg cgcctgaaac tggatgatgg cctcttgcag    720

gtggtggccc gtgacggcat cgaccttcca ggcatggaac gggcacagga actgctgctg    780

acccccggcg agcgggcaga tgtgctggtc tccttgcagg accaccagaa cctcaaactg    840

cagaacctgc cttacgaccg tggcgtgcac agcatgggga gccattcggg ccacacggag    900

agcaagacag ctgtggtcct gaccctgcag gggccacaga aacctggagc cagcgttccc    960

cgcgttgaaa ctgcacctcc acccttttta caactgcagg gcaatgaaaa agtgcggaaa   1020

gtgatcctgc aggaaaccat gcagcccgtg aagttcttca tcaacggacg cagcttcgac   1080

atgaaccggg tggatttcca tgtctccgag ggcagcaccg aaatctggga attgcagaac   1140

accaccgaaa tggaccaccc tttccacctc cacaccttcc caatgcagct gattcgcatc   1200

ggtggcaagg aggtccctcc ggtctggaag gacaccgtga acatcccgaa aaacagcacc   1260

gtcactgtgg ccgtgcactt caaaggcttc acgggcaaga ccgttttcca ctgtcacatc   1320

gccgagcatg aagaagccgg gatgatgggt cttcttcagg tgcatccctc tgggacagca   1380

ctcccggcct ctctggagcc cattgaaccc acagtgggtc caggagaaga cgcacaacac   1440

gggcac                                                              1446


<210>  2
<211>  482
<212>  PRT
<213>  Deinococcus sp.


<220>
<221>  MISC_FEATURE
<223>  Laccase 1 derived from Deinococcus cellulolyticus DRH46

<400>  2

Met Lys Gln Arg Leu Thr Ala Ala Ala Leu Leu Thr Ala Leu Leu Ser 
1               5                   10                  15      


Ser Cys Ala Met Lys Asn Pro Asp Val Ile Ser Tyr Gln Asp Pro Glu 
            20                  25                  30          


Pro Leu Ala Gly Gln Arg Ser Gly Lys Ala Ala Glu Phe Thr Leu Thr 
        35                  40                  45              


Ala Gln Lys Ser Arg Leu Asp Leu Ala Gly Gln Ser Val Glu Ala Leu 
    50                  55                  60                  


Thr Tyr Asn Gly Thr Phe Pro Gly Pro Leu Leu Thr Leu Lys Ala Gly 
65                  70                  75                  80  


Glu Asp Val Arg Ile Thr Leu Lys Asn Asn Leu Ser Glu Pro Thr Asn 
                85                  90                  95      


Leu His Leu His Gly Leu Pro Leu Ser Pro Glu Val Asp Asp Pro Phe 
            100                 105                 110         


Val Val Val Pro Pro Gly Gly Ser His Thr Tyr Ala Phe Gln Val Pro 
        115                 120                 125             


Glu Glu Val His Gly Thr Phe Trp Tyr His Thr His Thr His Gly Ala 
    130                 135                 140                 


Ser Ala Ala Gln Leu Phe Ser Gly Leu Ala Gly Pro Leu Leu Val Glu 
145                 150                 155                 160 


Ala Ala Lys Pro Asn Asn Pro Ile Glu His Met Glu Thr His Thr Val 
                165                 170                 175     


Val Phe Lys Asp Leu Pro Gly Ala Gln His Ala Asp Asp Gly Val Leu 
            180                 185                 190         


Met Asn Gly Arg Glu Asn Thr Leu Leu Val Asn Gly Leu Val Ser Pro 
        195                 200                 205             


Thr Leu Lys Thr Glu Asn Ser Trp Val Gln Leu Arg Leu Ile Asn Ala 
    210                 215                 220                 


Ser Asn Ala Arg Tyr Leu Arg Leu Lys Leu Asp Asp Gly Leu Leu Gln 
225                 230                 235                 240 


Val Val Ala Arg Asp Gly Ile Asp Leu Pro Gly Met Glu Arg Ala Gln 
                245                 250                 255     


Glu Leu Leu Leu Thr Pro Gly Glu Arg Ala Asp Val Leu Val Ser Leu 
            260                 265                 270         


Gln Asp His Gln Asn Leu Lys Leu Gln Asn Leu Pro Tyr Asp Arg Gly 
        275                 280                 285             


Val His Ser Met Gly Ser His Ser Gly His Thr Glu Ser Lys Thr Ala 
    290                 295                 300                 


Val Val Leu Thr Leu Gln Gly Pro Gln Lys Pro Gly Ala Ser Val Pro 
305                 310                 315                 320 


Arg Val Glu Thr Ala Pro Pro Pro Phe Leu Gln Leu Gln Gly Asn Glu 
                325                 330                 335     


Lys Val Arg Lys Val Ile Leu Gln Glu Thr Met Gln Pro Val Lys Phe 
            340                 345                 350         


Phe Ile Asn Gly Arg Ser Phe Asp Met Asn Arg Val Asp Phe His Val 
        355                 360                 365             


Ser Glu Gly Ser Thr Glu Ile Trp Glu Leu Gln Asn Thr Thr Glu Met 
    370                 375                 380                 


Asp His Pro Phe His Leu His Thr Phe Pro Met Gln Leu Ile Arg Ile 
385                 390                 395                 400 


Gly Gly Lys Glu Val Pro Pro Val Trp Lys Asp Thr Val Asn Ile Pro 
                405                 410                 415     


Lys Asn Ser Thr Val Thr Val Ala Val His Phe Lys Gly Phe Thr Gly 
            420                 425                 430         


Lys Thr Val Phe His Cys His Ile Ala Glu His Glu Glu Ala Gly Met 
        435                 440                 445             


Met Gly Leu Leu Gln Val His Pro Ser Gly Thr Ala Leu Pro Ala Ser 
    450                 455                 460                 


Leu Glu Pro Ile Glu Pro Thr Val Gly Pro Gly Glu Asp Ala Gln His 
465                 470                 475                 480 


Gly His 
        


<210>  3
<211>  1476
<212>  DNA
<213>  Deinococcus sp.


<220>
<221>  misc_feature
<223>  Laccase 2 derived from Deinococcus cellulolyticus DRH46

<400>  3
atgattcgaa ccctcacttc cctcaccctg ctgaccgtgg cgctcaccgc atgcgctcca     60

aaaggaagtc ccatgaccga gtaccacaac cctgctgaag tgcaagcaag ccgcacagaa    120

aaaggcagca ccttcaccct caccgccgca gaaaccagca tcaccctgga cggcaaagag    180

gtgaaagcac agctcttcaa ccagagtttt cccggacccc agctcaccct gcaggccgga    240

gaacaggtga acctcaccct caaaaacgac ctccgtgaac ccaccaacct gcacctgcac    300

ggcctgaccc tcccggcagg ggtggacgat ccctttctgc aggtccagcc cggagaaagc    360

cacacctaca gcttcactgt tccgcaaggc ctgcacggca ccttctggta ccacacccac    420

acccatgggc acctcggaac acaactcttc cagggcctcg caggaccctt gatcgtcgag    480

gaccccacct ttgacagcca catccagggg atggagaccc acaccgtggt gctcaaagac    540

ctccctgttg aagtacagga cagtgacggc acccgcatga acggccggga aggcaaactg    600

ctggtcaacg gcctctcctc ccccaccctg aagaccgaaa gcagctgggt gaggctccgc    660

ctgatcaacg cttcaaacgc ccgctatgac ctgctgaaac tctctggttc agcttttctc    720

cacgtcatcg ccaaggacgg gatcgacctg cccaaaaccg agcaggtgaa agaactcctc    780

ctcacccccg gcgagcgggc cgatgtgctg atttccgtgc aagaccagca ggacctccgg    840

ctggaaaacc acccttacga ccggggggtg catgagatgg acggggggca tcagggtcac    900

acacccagca caccagaggt gctcctcacc ctgcagggga agaaagggag cgtctcagcc    960

agcgtgccac aacctgccct gcagccccgt ccattcctga aggtgactgg caacgaagcc   1020

attcgcaagg tggtgctgca agaaaccatg cagcctgtga aattcttcat caacggacgc   1080

acgtttgaca tgaaccgggt ggatttccat gtctctgaag gcaccaccga aatctgggag   1140

gtggagaacc agaccgaaat ggaccacccc ttccacctgc acaccttccc ggtgcaggtc   1200

ctctcggtga acggccagga agtgcctccg gtgtggaagg acaccgtgaa cgtccctgcg   1260

aaagcaaaag tgcaggtggc cgtgaaattt gagggcttca ccggcaagac ggtcttccac   1320

tgccacattg ccgagcacga agaagagggc atgatgggcg tcttgcaggt gcatccagca   1380

ggtgaggcgc tccctgcgtc tctcacaccc acccttcccc ctgcagctgc ttcggaggac   1440

atgcctgaca tgcccggcat ggaccacagt ggccac                             1476


<210>  4
<211>  492
<212>  PRT
<213>  Deinococcus sp.


<220>
<221>  MISC_FEATURE
<223>  Laccase 2 derived from Deinococcus cellulolyticus DRH46

<400>  4

Met Ile Arg Thr Leu Thr Ser Leu Thr Leu Leu Thr Val Ala Leu Thr 
1               5                   10                  15      


Ala Cys Ala Pro Lys Gly Ser Pro Met Thr Glu Tyr His Asn Pro Ala 
            20                  25                  30          


Glu Val Gln Ala Ser Arg Thr Glu Lys Gly Ser Thr Phe Thr Leu Thr 
        35                  40                  45              


Ala Ala Glu Thr Ser Ile Thr Leu Asp Gly Lys Glu Val Lys Ala Gln 
    50                  55                  60                  


Leu Phe Asn Gln Ser Phe Pro Gly Pro Gln Leu Thr Leu Gln Ala Gly 
65                  70                  75                  80  


Glu Gln Val Asn Leu Thr Leu Lys Asn Asp Leu Arg Glu Pro Thr Asn 
                85                  90                  95      


Leu His Leu His Gly Leu Thr Leu Pro Ala Gly Val Asp Asp Pro Phe 
            100                 105                 110         


Leu Gln Val Gln Pro Gly Glu Ser His Thr Tyr Ser Phe Thr Val Pro 
        115                 120                 125             


Gln Gly Leu His Gly Thr Phe Trp Tyr His Thr His Thr His Gly His 
    130                 135                 140                 


Leu Gly Thr Gln Leu Phe Gln Gly Leu Ala Gly Pro Leu Ile Val Glu 
145                 150                 155                 160 


Asp Pro Thr Phe Asp Ser His Ile Gln Gly Met Glu Thr His Thr Val 
                165                 170                 175     


Val Leu Lys Asp Leu Pro Val Glu Val Gln Asp Ser Asp Gly Thr Arg 
            180                 185                 190         


Met Asn Gly Arg Glu Gly Lys Leu Leu Val Asn Gly Leu Ser Ser Pro 
        195                 200                 205             


Thr Leu Lys Thr Glu Ser Ser Trp Val Arg Leu Arg Leu Ile Asn Ala 
    210                 215                 220                 


Ser Asn Ala Arg Tyr Asp Leu Leu Lys Leu Ser Gly Ser Ala Phe Leu 
225                 230                 235                 240 


His Val Ile Ala Lys Asp Gly Ile Asp Leu Pro Lys Thr Glu Gln Val 
                245                 250                 255     


Lys Glu Leu Leu Leu Thr Pro Gly Glu Arg Ala Asp Val Leu Ile Ser 
            260                 265                 270         


Val Gln Asp Gln Gln Asp Leu Arg Leu Glu Asn His Pro Tyr Asp Arg 
        275                 280                 285             


Gly Val His Glu Met Asp Gly Gly His Gln Gly His Thr Pro Ser Thr 
    290                 295                 300                 


Pro Glu Val Leu Leu Thr Leu Gln Gly Lys Lys Gly Ser Val Ser Ala 
305                 310                 315                 320 


Ser Val Pro Gln Pro Ala Leu Gln Pro Arg Pro Phe Leu Lys Val Thr 
                325                 330                 335     


Gly Asn Glu Ala Ile Arg Lys Val Val Leu Gln Glu Thr Met Gln Pro 
            340                 345                 350         


Val Lys Phe Phe Ile Asn Gly Arg Thr Phe Asp Met Asn Arg Val Asp 
        355                 360                 365             


Phe His Val Ser Glu Gly Thr Thr Glu Ile Trp Glu Val Glu Asn Gln 
    370                 375                 380                 


Thr Glu Met Asp His Pro Phe His Leu His Thr Phe Pro Val Gln Val 
385                 390                 395                 400 


Leu Ser Val Asn Gly Gln Glu Val Pro Pro Val Trp Lys Asp Thr Val 
                405                 410                 415     


Asn Val Pro Ala Lys Ala Lys Val Gln Val Ala Val Lys Phe Glu Gly 
            420                 425                 430         


Phe Thr Gly Lys Thr Val Phe His Cys His Ile Ala Glu His Glu Glu 
        435                 440                 445             


Glu Gly Met Met Gly Val Leu Gln Val His Pro Ala Gly Glu Ala Leu 
    450                 455                 460                 


Pro Ala Ser Leu Thr Pro Thr Leu Pro Pro Ala Ala Ala Ser Glu Asp 
465                 470                 475                 480 


Met Pro Asp Met Pro Gly Met Asp His Ser Gly His 
                485                 490         


<210>  5
<211>  814
<212>  DNA
<213>  Deinococcus sp.


<220>
<221>  misc_feature
<223>  Laccase 3 derived from Deinococcus maricopensis DSM 21211

<400>  5
atgacgctcc atactgacct gatgctgctg cgctccccgc acctcacgtt cccgcacgcg     60

ttctcgacgc gggcgggcgg cgtctcgagt ggcccgtacg cgggcctgaa cctcgacgac    120

cgcgaggacg acgcgcacag cgtcgccgag aaccgccgcc gcctcaccac gcagctcggg    180

ttcacgcccg cacgcgtagc gagcctcaca caggtccacg gcgcggacgt cgccgtggcg    240

agcgcgcccg gcgtgcacag cggtgacgcc atcgtcaccg cccagccgga cctgctgctc    300

gccatcatga cggccgactg ctacccgctg ctccttgagg acgccgaggc cggcgtgatc    360

ggcgcggcgc acgccggatg gcgcggcacg gtcgcccgca tcggcgcgcg caccattgag    420

gccatgaccc gcctgggcgc ccggccggaa cgcatccgcg ccgcggtcgg ccccggcatc    480

tgtggcgcgc ggtacgccgt cggcgaggac gtcgccgcgc agttccgcgc cgcgggcctc    540

ggggacgcgc tgagcgggct gcagctggac ctcgcgcggg cgaacacaca ggtgctcgtc    600

gacgcgggcg tctccgcgtc gcacctgtgg gtgtcggggc gctgcaccac cgaaccggac    660

ttctactcgt accgacggga tgccggacgc accgggcgga tgtgggccct gatcggccgc    720

ggaggcgcag aacctcacgg tgtgcgccgc gcgcccgacc ctaaaatggt gggtgctttg    780

gacacccaca cctctgccgg ggagggccgc gcgc                                814


<210>  6
<211>  271
<212>  PRT
<213>  Deinococcus sp.


<220>
<221>  MISC_FEATURE
<223>  Laccase 3 derived from Deinococcus maricopensis DSM 21211

<400>  6

Met Thr Leu His Thr Asp Leu Met Leu Leu Arg Ser Pro His Leu Thr 
1               5                   10                  15      


Phe Pro His Ala Phe Ser Thr Arg Ala Gly Gly Val Ser Ser Gly Pro 
            20                  25                  30          


Tyr Ala Gly Leu Asn Leu Asp Asp Arg Glu Asp Asp Ala His Ser Val 
        35                  40                  45              


Ala Glu Asn Arg Arg Arg Leu Thr Thr Gln Leu Gly Phe Thr Pro Ala 
    50                  55                  60                  


Arg Val Ala Ser Leu Thr Gln Val His Gly Ala Asp Val Ala Val Ala 
65                  70                  75                  80  


Ser Ala Pro Gly Val His Ser Gly Asp Ala Ile Val Thr Ala Gln Pro 
                85                  90                  95      


Asp Leu Leu Leu Ala Ile Met Thr Ala Asp Cys Tyr Pro Leu Leu Leu 
            100                 105                 110         


Glu Asp Ala Glu Ala Gly Val Ile Gly Ala Ala His Ala Gly Trp Arg 
        115                 120                 125             


Gly Thr Val Ala Arg Ile Gly Ala Arg Thr Ile Glu Ala Met Thr Arg 
    130                 135                 140                 


Leu Gly Ala Arg Pro Glu Arg Ile Arg Ala Ala Val Gly Pro Gly Ile 
145                 150                 155                 160 


Cys Gly Ala Arg Tyr Ala Val Gly Glu Asp Val Ala Ala Gln Phe Arg 
                165                 170                 175     


Ala Ala Gly Leu Gly Asp Ala Leu Ser Gly Leu Gln Leu Asp Leu Ala 
            180                 185                 190         


Arg Ala Asn Thr Gln Val Leu Val Asp Ala Gly Val Ser Ala Ser His 
        195                 200                 205             


Leu Trp Val Ser Gly Arg Cys Thr Thr Glu Pro Asp Phe Tyr Ser Tyr 
    210                 215                 220                 


Arg Arg Asp Ala Gly Arg Thr Gly Arg Met Trp Ala Leu Ile Gly Arg 
225                 230                 235                 240 


Gly Gly Ala Glu Pro His Gly Val Arg Arg Ala Pro Asp Pro Lys Met 
                245                 250                 255     


Val Gly Ala Leu Asp Thr His Thr Ser Ala Gly Glu Gly Arg Ala 
            260                 265                 270     


<210>  7
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  6(His) tag

<400>  7
catcatcacc atcaccatta a                                               21


<210>  8
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  primer 16S rDNA

<400>  8
gttacccgga atcactgggc gta                                             23


<210>  9
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  primer 16S rDNA

<400>  9
ggtatctacg cattccaccg cta                                             23


