                         SEQUENCE LISTING

<110>  FUJIFILM Diosynth Biotechnologies UK Limited
 
<120>  Expression Process

<130>  BIL 81015/WO

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  141
<212>  PRT
<213>  Homo sapiens

<400>  1

Ser Tyr Ala Arg Gln Glu Gly Lys Asp Arg Ile Ile Phe Val Thr Lys 
1               5                   10                  15      


Glu Asp His Glu Thr Pro Ser Ser Ala Glu Leu Val Ala Asp Asp Pro 
            20                  25                  30          


Asn Asp Pro Tyr Glu Glu His Gly Leu Ile Leu Pro Asn Gly Asn Ile 
        35                  40                  45              


Asn Trp Asn Cys Pro Cys Leu Gly Gly Met Ala Ser Gly Pro Cys Gly 
    50                  55                  60                  


Glu Gln Phe Lys Ser Ala Phe Ser Cys Phe His Tyr Ser Thr Glu Glu 
65                  70                  75                  80  


Ile Lys Gly Ser Asp Cys Val Asp Gln Phe Arg Ala Met Gln Glu Cys 
                85                  90                  95      


Met Gln Lys Tyr Pro Asp Leu Tyr Pro Gln Glu Asp Glu Asp Glu Glu 
            100                 105                 110         


Glu Glu Arg Glu Lys Lys Pro Ala Glu Gln Ala Glu Glu Thr Ala Pro 
        115                 120                 125             


Ile Glu Ala Thr Ala Thr Lys Glu Glu Glu Gly Ser Ser 
    130                 135                 140     


<210>  2
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence leader 1

<400>  2

Met Lys Val Ser Thr Ala Phe Leu Cys Leu Leu Leu Thr Val Ser Ala 
1               5                   10                  15      


Phe Ser Ala Gln Val Leu Ala 
            20              


<210>  3
<211>  105
<212>  PRT
<213>  Escherichia coli

<400>  3

Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp Val Leu Lys Ala 
1               5                   10                  15      


Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp Ser Gly Pro Ser 
            20                  25                  30          


Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp Glu Tyr Gln Gly 
        35                  40                  45              


Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala 
    50                  55                  60                  


Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Lys Asn 
65                  70                  75                  80  


Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser Lys Gly Gln Leu 
                85                  90                  95      


Lys Glu Phe Leu Asp Ala Asn Leu Ala 
            100                 105 


<210>  4
<211>  276
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Thioredoxin fusion protein

<400>  4

Met Lys Val Ser Thr Ala Phe Leu Cys Leu Leu Leu Thr Val Ser Ala 
1               5                   10                  15      


Phe Ser Ala Gln Val Leu Ala Asp Tyr Lys Asp Glu Asp Lys Ile Ile 
            20                  25                  30          


His Leu Thr Asp Asp Ser Phe Asp Thr Asp Val Leu Lys Ala Asp Gly 
        35                  40                  45              


Ala Ile Leu Val Asp Phe Trp Ala Glu Trp Ser Gly Pro Ser Lys Met 
    50                  55                  60                  


Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp Glu Tyr Gln Gly Lys Leu 
65                  70                  75                  80  


Thr Val Ala Lys Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala Pro Lys 
                85                  90                  95      


Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Lys Asn Gly Glu 
            100                 105                 110         


Val Ala Ala Thr Lys Val Gly Ala Leu Ser Lys Gly Gln Leu Lys Glu 
        115                 120                 125             


Phe Leu Asp Ala Asn Leu Ala Ser Tyr Ala Arg Gln Glu Gly Lys Asp 
    130                 135                 140                 


Arg Ile Ile Phe Val Thr Lys Glu Asp His Glu Thr Pro Ser Ser Ala 
145                 150                 155                 160 


Glu Leu Val Ala Asp Asp Pro Asn Asp Pro Tyr Glu Glu His Gly Leu 
                165                 170                 175     


Ile Leu Pro Asn Gly Asn Ile Asn Trp Asn Cys Pro Cys Leu Gly Gly 
            180                 185                 190         


Met Ala Ser Gly Pro Cys Gly Glu Gln Phe Lys Ser Ala Phe Ser Cys 
        195                 200                 205             


Phe His Tyr Ser Thr Glu Glu Ile Lys Gly Ser Asp Cys Val Asp Gln 
    210                 215                 220                 


Phe Arg Ala Met Gln Glu Cys Met Gln Lys Tyr Pro Asp Leu Tyr Pro 
225                 230                 235                 240 


Gln Glu Asp Glu Asp Glu Glu Glu Glu Arg Glu Lys Lys Pro Ala Glu 
                245                 250                 255     


Gln Ala Glu Glu Thr Ala Pro Ile Glu Ala Thr Ala Thr Lys Glu Glu 
            260                 265                 270         


Glu Gly Ser Ser 
        275     


<210>  5
<211>  986
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Thioredoxin fusion protein gene

<400>  5
gcggccgcga tatcacgcgt ttaggcaccc caggctttac actttatgct tccggctggt       60

atgttgtgtg gactttaaga aggagatata catatgaagg taagcaccgc atttttgtgc      120

ctgttattga cggtcagcgc attcagcgca caagtcctgg cggactataa ggacgaggac      180

aagatcattc acctgaccga tgactcgttc gacaccgacg tgctgaaagc tgatggtgcc      240

atcctggtcg acttctgggc ggagtggagc ggtccgagca agatgattgc gccgatcctg      300

gatgagattg cagatgaata ccagggcaaa ctgacggttg caaaattgaa cattgatcaa      360

aacccgggca cggctccgaa gtatggcatt cgtggtatcc cgaccctgct gctgttcaag      420

aatggtgagg tggcggcgac caaggttggc gcgctgtcca agggtcaact gaaagagttt      480

ctggacgcta atttggcaag ctacgcgcgt caggaaggta aagatcgcat tatctttgtt      540

accaaagagg atcatgaaac cccgagcagc gcggagttgg ttgcggacga tccgaacgat      600

ccgtacgaag agcatggcct gatcctgccg aatggtaaca tcaattggaa ctgcccgtgc      660

ctgggtggca tggccagcgg tccgtgcggc gagcagttca aatcagcatt tagctgtttt      720

cactacagca ctgaagagat caaaggtagc gactgcgtgg accagttccg tgccatgcag      780

gagtgtatgc aaaagtatcc agacctgtac cctcaggaag atgaggatga agaagaagag      840

cgcgagaaga aaccggcgga gcaagccgaa gaaacggccc caattgaggc aaccgcgacg      900

aaagaagaag agggttcttc ctaatgaatt atattactaa ttaattgggg accctagagg      960

tccccttttt tattttaaaa ctcgag                                           986


<210>  6
<211>  204
<212>  PRT
<213>  Homo sapiens

<400>  6

Ala Ala Pro Gly Glu Arg Gly Arg Phe His Gly Gly Asn Leu Phe Phe 
1               5                   10                  15      


Leu Pro Gly Gly Ala Arg Ser Glu Met Met Asp Asp Leu Ala Thr Asp 
            20                  25                  30          


Ala Arg Gly Arg Gly Ala Gly Arg Arg Asp Ala Ala Ala Ser Ala Ser 
        35                  40                  45              


Thr Pro Ala Gln Ala Pro Thr Ser Asp Ser Pro Val Ala Glu Asp Ala 
    50                  55                  60                  


Ser Arg Arg Arg Pro Cys Arg Ala Cys Val Asp Phe Lys Thr Trp Met 
65                  70                  75                  80  


Arg Thr Gln Gln Lys Arg Asp Thr Lys Phe Arg Glu Asp Cys Pro Pro 
                85                  90                  95      


Asp Arg Glu Glu Leu Gly Arg His Ser Trp Ala Val Leu His Thr Leu 
            100                 105                 110         


Ala Ala Tyr Tyr Pro Asp Leu Pro Thr Pro Glu Gln Gln Gln Asp Met 
        115                 120                 125             


Ala Gln Phe Ile His Leu Phe Ser Lys Phe Tyr Pro Cys Glu Glu Cys 
    130                 135                 140                 


Ala Glu Asp Leu Arg Lys Arg Leu Cys Arg Asn His Pro Asp Thr Arg 
145                 150                 155                 160 


Thr Arg Ala Cys Phe Thr Gln Trp Leu Cys His Leu His Asn Glu Val 
                165                 170                 175     


Asn Arg Lys Leu Gly Lys Pro Asp Phe Asp Cys Ser Lys Val Asp Glu 
            180                 185                 190         


Arg Trp Arg Asp Gly Trp Lys Asp Gly Ser Cys Asp 
        195                 200                 


<210>  7
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ALRP fusion protein

<400>  7

Met Lys Val Ser Thr Ala Phe Leu Cys Leu Leu Leu Thr Val Ser Ala 
1               5                   10                  15      


Phe Ser Ala Gln Val Leu Ala Asp Tyr Lys Asp Asp Asp Asp Lys Ala 
            20                  25                  30          


Ala Pro Gly Glu Arg Gly Arg Phe His Gly Gly Asn Leu Phe Phe Leu 
        35                  40                  45              


Pro Gly Gly Ala Arg Ser Glu Met Met Asp Asp Leu Ala Thr Asp Ala 
    50                  55                  60                  


Arg Gly Arg Gly Ala Gly Arg Arg Asp Ala Ala Ala Ser Ala Ser Thr 
65                  70                  75                  80  


Pro Ala Gln Ala Pro Thr Ser Asp Ser Pro Val Ala Glu Asp Ala Ser 
                85                  90                  95      


Arg Arg Arg Pro Cys Arg Ala Cys Val Asp Phe Lys Thr Trp Met Arg 
            100                 105                 110         


Thr Gln Gln Lys Arg Asp Thr Lys Phe Arg Glu Asp Cys Pro Pro Asp 
        115                 120                 125             


Arg Glu Glu Leu Gly Arg His Ser Trp Ala Val Leu His Thr Leu Ala 
    130                 135                 140                 


Ala Tyr Tyr Pro Asp Leu Pro Thr Pro Glu Gln Gln Gln Asp Met Ala 
145                 150                 155                 160 


Gln Phe Ile His Leu Phe Ser Lys Phe Tyr Pro Cys Glu Glu Cys Ala 
                165                 170                 175     


Glu Asp Leu Arg Lys Arg Leu Cys Arg Asn His Pro Asp Thr Arg Thr 
            180                 185                 190         


Arg Ala Cys Phe Thr Gln Trp Leu Cys His Leu His Asn Glu Val Asn 
        195                 200                 205             


Arg Lys Leu Gly Lys Pro Asp Phe Asp Cys Ser Lys Val Asp Glu Arg 
    210                 215                 220                 


Trp Arg Asp Gly Trp Lys Asp Gly Ser Cys Asp 
225                 230                 235 


<210>  8
<211>  861
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ALRP fusion protein gene

<400>  8
gatatcacgc gtttaggcac cccaggcttt acactttatg cttccggctg gtatgttgtg       60

tggactttaa gaaggagata tacatatgaa agtgagcacc gcattcttgt gtctgctgtt      120

gacggtctcc gcgttcagcg cacaagtttt ggcggactat aaggatgacg atgacaaagc      180

ggcaccgggc gaacgcggtc gtttccacgg tggcaatttg tttttcctgc cgggcggcgc      240

ccgcagcgag atgatggacg acttggcgac tgacgcgcgt ggtcgtggtg ctggtcgtcg      300

tgatgcagcg gcctcggcaa gcacgccggc tcaggcccca acctccgata gcccggtggc      360

ggaggacgct agccgccgtc gcccgtgtcg tgcgtgtgtt gactttaaaa cctggatgcg      420

tacccaacag aagcgtgaca ccaagtttcg cgaagactgc ccgccggacc gtgaggaact      480

gggtcgccac tcttgggcgg tcctgcacac gctggcggcg tactacccgg atctgcctac      540

gccggaacag cagcaggata tggcgcaatt catccatctg tttagcaagt tttatccttg      600

cgaggagtgt gccgaagatt tgcgtaagcg cctgtgtcgt aaccatccgg acacccgtac      660

ccgtgcctgc tttacccaat ggttgtgcca cctgcacaat gaagttaacc gcaaattggg      720

caaaccggat ttcgactgca gcaaagtgga tgagcgttgg cgtgatggtt ggaaggatgg      780

tagctgcgat taatgaatta tattactaat taattgggga ccctagaggt cccctttttt      840

attttaaaac tcgaggatat c                                                861


<210>  9
<211>  300
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IGF plus leader gene

<400>  9
catatgatga ttaccctgcg taaactgccg ctggcagtcg cagttgcagc cggtgttatg       60

agcgcacagg caatggcagg tccggaaacc ctgtgtggtg cagaactggt tgatgcactg      120

cagtttgttt gtggtgatcg tggcttttat tttaataaac cgaccggtta tggtagcagc      180

agccgtcgtg cacgtcagac cggtattgtt gatgaatgtt gctttcgtag ctgtgatctg      240

cgtcgtctgg aaatgtattg tgcaccgctg aaaccggcaa aaagcgcata ataactcgag      300


<210>  10
<211>  651
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hGH plus leader gene

<400>  10
catatgaaaa agactgctat tgctattgcg gttgctctgg ctggtttcgc caccgtggcg       60

caggcattcc cgacgattcc gctgtctcgt ctgttcgata acgctatgct gcgtgcacat      120

cgtctgcacc agctggcatt cgacacttat caagaattcg aggaggccta catcccgaaa      180

gaacagaaat acagcttcct gcagaacccg caaacctccc tgtgcttcag cgaatccatc      240

ccgactccgt ctaaccgcga agaaacccag cagaaatcta atctggaact gctgcgtatt      300

agcctgctgc tgatccagtc ttggctggaa ccggtgcaat tcctgcgcag cgtcttcgcg      360

aactccctgg tatatggcgc ttccgactct aacgtatacg atctgctgaa agacctggag      420

gagggtatcc agaccctgat gggccgcctg gaggacggca gcccgcgtac tggccagatc      480

tttaaacaga cttactctaa attcgacacc aactcccata atgatgacgc cctgctgaaa      540

aactatggtc tgctgtactg cttccgtaaa gacatggaca aagttgagac cttcctgcgt      600

attgttcagt gtcgctctgt tgagggttct tgcggcttct aatgactcga g               651


<210>  11
<211>  1551
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A5B7 Fab gene

<400>  11
catatgaaga agactgctat tgctattgcg gttgcactgg ctggtttcgc taccgtggcg       60

caggcacagg ttcagctgca ggaaagcggc ggtggtctgg ttcagccagg cggtagcctg      120

cgtctgtcct gcgctaccag cggtttcact tttaccgact actacatgaa ctgggtgcgt      180

cagccgccag gcaaagcgct ggaatggctg ggcttcatcg gtaacaaagc taacggctac      240

actaccgagt actccgcatc tgtaaaaggt cgttttacca tctctcgtga caaaagccag      300

tctatcctgt acctgcagat gaataccctg cgcgctgaag actccgcgac ttattactgt      360

acccgtgacc gtggtctgcg tttttacttc gactactggg gtcagggtac cactgtaacg      420

gtcagctccg cctccactaa aggcccgagc gtatttcctc tggcgccgtc ctccaagtcc      480

actagcggcg gcaccgcagc tctgggctgt ctggttaaag actacttccc agagccggtg      540

accgtttcct ggaacagcgg tgcactgacc agcggcgttc acacgtttcc ggccgtgctg      600

caatcctccg gtctgtactc tctgtcttcc gtcgtcaccg ttccgtctag ctctctgggc      660

actcagacct atatctgcaa cgtcaatcac aacccgtcta acaccaaagt tgacaagaaa      720

gtggagccga aatcttgcga taaaacgcac acctaataac tggccctgag ctccaagttc      780

tactttaaag aaacggttat catgaaaaag accgccatcg ctatcgctgt tgcgctggca      840

ggctttgcta cggttgctca agctgacatc gaactgtctc agtctccggc gatcctgtcc      900

gcgtctccag gcgaaaaagt aactatgacc tgtcgcgcta gctcttccgt tacctacatc      960

cactggtatc agcagaaacc gggttcttcc ccgaaaagct ggatctacgc aacctccaac     1020

ctggcttccg gcgttccggc tcgtttttct ggttctggct ctggcacgtc ctattctctg     1080

accatcagcc gtgtagaggc agaagacgca gctacctatt actgccagca ctggtcctcc     1140

aaacctccga ctttcggtgg cggcaccaaa ctggaaatca agcgtaccgt cgccgctccg     1200

tctgttttca ttttcccgcc gtccgacgag cagctgaaat ccggtaccgc cagcgtagtg     1260

tgcctgctga acaacttcta cccacgtgag gctaaagttc agtggaaagt ggacaatgcc     1320

ctgcagagcg gcaactccca ggaatccgtt accgaacagg acagcaaaga ttccacttat     1380

tccctgtctt ccactctgac tctgagcaaa gcagattacg agaagcataa ggtgtacgcc     1440

tgtgaagtga ctcaccaggg tctgtcctct ccggttacga aaagctttaa ccgtggcgag     1500

tgctaataag gatccagctc gaattccatc gatgatatca gatctctcga g              1551


<210>  12
<211>  621
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FGF21 plus leader gene

<400>  12
catatgaaaa aaaccgctat tgcaattgca gttgcactgg caggttttgc aaccgttgca       60

caggcacatc cgattccgga tagcagtccg ctgctgcagt ttggtggtca ggttcgtcag      120

cgttatctgt ataccgatga tgcacagcag accgaagcac atctggaaat tcgtgaagat      180

ggcaccgttg gtggtgcagc agatcagagt ccggaaagcc tgctgcagct gaaagcactg      240

aaaccgggtg ttattcagat tctgggtgtt aaaaccagcc gttttctgtg tcagcgtccg      300

gatggtgcac tgtatggtag cctgcatttt gatccggaag catgtagctt tcgtgaactg      360

ctgctggaag atggttataa tgtttatcag agcgaacatc atggtctgcc actgcatctg      420

cctggtaata aaagtccgca tcgtgatccg gcaccgcgtg gtccggcacg ttttctgccg      480

ctgcctggtc tgcctcctgc actgccggaa cctccgggta ttctggcacc gcagcctccg      540

gatgttggta gcagcgatcc gctgagcatg gttggtccga gccagggtcg tagcccgagc      600

tatgcaagct aataactcga g                                                621


<210>  13
<211>  488
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RapLR plus leader gene

<400>  13
gaaggccgtc aaggccacgt gtcttgtcca ggtaccatat gaaaaaaacc gccattgcaa       60

ttgcagttgc tctggcaggt tttgcaaccg ttgcacaggc acaggattgg ctgacctttc      120

agaaaaaaca tctgaccaat acccgtgatg tggattgcaa taatattatg agcaccaacc      180

tgtttcattg caaagataaa aataccttta tttatagccg tccggaaccg gttaaagcaa      240

tttgtaaagg tattattgcc agcaaaaatg tgctgaccac gagcgagttt tatctgagcg      300

attgtaatgt taccagccgt ccgtgtaaat ataaactgaa aaaaagcacc aatacctttt      360

gcgtgacctg tgaaaatcag gcaccggttc attttgttgg tgttggtcat tgtgaacaga      420

aactgattag cgaagaagat ctgtaataac tcgagctctg gagcacaaga ctggcctcat      480

gggccttc                                                               488


<210>  14
<211>  279
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Elafin plus leader gene

<400>  14
catatgaaaa aaaccgccat tgcaattgca gttgctctgg caggttttgc aaccgttgca       60

caggcagcac aggaaccggt taaaggtccg gttagcacca aaccgggtag ctgtccgatt      120

attctgattc gttgtgcaat gctgaatcct ccgaatcgtt gtctgaaaga taccgattgt      180

ccgggtatta aaaaatgttg tgaaggtagc tgtggcatgg catgttttgt tccgcaggaa      240

cagaaactga ttagcgaaga agatctgtaa taactcgag                             279


<210>  15
<211>  1152
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MIC6 plus leader gene

<400>  15
catatgaaaa aaaccgccat tgcaattgca gttgctctgg caggttttgc aaccgttgca       60

caggcacgtc tgtttcgttg ttgtgcagca gcagttgttg cagcagaaag cctgctgtgg      120

ctgaaaaatg gtagcccgtt ttttgcattt ctgcctggta atggtgaaat tgcagataat      180

tgtagcggta atccgtgtgg tggcaccgca gcaggcacct gtattaatac cccgagcggt      240

tatgattgtc gttgtgaacc gggttatgtt ctgggtgttg aaaatgatca ggttacctgt      300

atgatgccga gcggtgttcc gatggcaaat tttgttcagc tgagcgaaaa accggcagca      360

tgtagcagca atccgtgcgg tccggaagca gcaggtacat gtaatgaaac caatagcggt      420

tatatttgcc gttgcaatca gggttatcgt attagcctgg atggcaccgg taatgttacc      480

tgtattgttc gtcaggaaag cggttgcgaa gaaaatggtt gtggtccgcc ggatgccgtt      540

cagagctgtc gtcgtctgac cggcacagca ggtcgtctgt gtgtttgtaa agaaaatttt      600

attgccacca ttgatgccag cgcacatatt acctgtaaac gtgttccgcc gcattatcgt      660

aaaccgccgt ttgaatttgg taaaggtggt catccggttg atagcgaacc gagcaaacgt      720

cagcgtgaag atgaaggtga aagccgtgaa ccggaaagcg atagcaccga accgggtcgt      780

gatcaggaac gtcgtacacc gctggaagaa agccaggaac cggaaggtag cacaccggat      840

agccagcaga gccgtggtgg tagcggtagc gatagtaccg aaagcgaaga acagggtaaa      900

gaacgtgaag aaggtagcgg tcacgccggt gcaattgccg gtggtgttat tggtggtctg      960

ctgctgctga gcgcagccgg tgccggtgtt gcatatatgc gtaaaagcgg tagcggtggt     1020

ggtgaagaaa ttgaatatga acgtggtatt gaagcagcag aagcaagcga agttgaagtt     1080

ctggttgatc tggatagcaa aacctgggat gaacagaaac tgattagcga agaagatctg     1140

taataactcg ag                                                         1152


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tag

<400>  16

Asp Tyr Lys Asp Glu Asp Lys 
1               5           


<210>  17
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tag

<400>  17

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


