                         SEQUENCE LISTING

<110>  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE 
       (INSERM)
 
<120>  VACCINES FOR PREVENTING MENINGOCOCCAL INFECTIONS

<130>  BET11P2899

<150>  EP 10306215.4
<151>  2010-11-05

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  168
<212>  PRT
<213>  Neisseria meningitidis

<400>  1

Met Asn Thr Leu Gln Lys Gly Phe Thr Leu Ile Glu Leu Met Ile Val 
1               5                   10                  15      


Ile Ala Ile Val Gly Ile Leu Ala Ala Val Ala Leu Pro Ala Tyr Gln 
            20                  25                  30          


Asp Tyr Thr Ala Arg Ala Gln Val Ser Glu Ala Ile Leu Leu Ala Glu 
        35                  40                  45              


Gly Gln Lys Ser Ala Val Thr Glu Tyr Tyr Leu Asn His Gly Glu Trp 
    50                  55                  60                  


Pro Gly Asp Asn Ser Ser Ala Gly Val Ala Thr Ser Ala Asp Ile Lys 
65                  70                  75                  80  


Gly Lys Tyr Val Gln Ser Val Thr Val Ala Asn Gly Val Ile Thr Ala 
                85                  90                  95      


Gln Met Ala Ser Ser Asn Val Asn Asn Glu Ile Lys Ser Lys Lys Leu 
            100                 105                 110         


Ser Leu Trp Ala Lys Arg Gln Asn Gly Ser Val Lys Trp Phe Cys Gly 
        115                 120                 125             


Gln Pro Val Thr Arg Thr Thr Ala Thr Ala Thr Asp Val Ala Ala Ala 
    130                 135                 140                 


Asn Gly Lys Thr Asp Asp Lys Ile Asn Thr Lys His Leu Pro Ser Thr 
145                 150                 155                 160 


Cys Arg Asp Asp Ser Ser Ala Ser 
                165             


<210>  2
<211>  129
<212>  PRT
<213>  Neisseria meningitidis

<400>  2

Met Lys Asn Val Gln Lys Gly Phe Thr Leu Leu Glu Leu Met Ile Ala 
1               5                   10                  15      


Val Ala Ile Leu Gly Ile Leu Thr Leu Ile Thr Tyr Pro Ser Tyr Lys 
            20                  25                  30          


Thr Tyr Ile Arg Arg Val Arg Leu Ser Glu Val Arg Thr Thr Leu Leu 
        35                  40                  45              


His Asn Ala Gln Thr Met Glu Arg Tyr Tyr Arg Gln Lys Gly Thr Phe 
    50                  55                  60                  


Lys Thr Tyr Asp Lys Asn Lys Leu Lys Gln Asn Lys Tyr Phe Asn Val 
65                  70                  75                  80  


Thr Leu Ser Lys Val Ser Pro Asp His Phe Thr Leu Gln Ala Asp Pro 
                85                  90                  95      


Asn Pro Thr Thr Asn Asp Gly Glu Thr Cys Val Val Thr Leu Asn Asp 
            100                 105                 110         


Gly Gly Thr Ile Ala Ala Ser Gly Thr Asn Gln Ser Cys Pro Gly Phe 
        115                 120                 125             


Asp 
    


<210>  3
<211>  413
<212>  PRT
<213>  Homo sapiens


<220>
<221>  DOMAIN
<222>  (1)..(28)
<223>  N-terminus

<400>  3

Met Gly Gln Pro Gly Asn Gly Ser Ala Phe Leu Leu Ala Pro Asn Gly 
1               5                   10                  15      


Ser His Ala Pro Asp His Asp Val Thr Gln Glu Arg Asp Glu Val Trp 
            20                  25                  30          


Val Val Gly Met Gly Ile Val Met Ser Leu Ile Val Leu Ala Ile Val 
        35                  40                  45              


Phe Gly Asn Val Leu Val Ile Thr Ala Ile Ala Lys Phe Glu Arg Leu 
    50                  55                  60                  


Gln Thr Val Thr Asn Tyr Phe Ile Thr Ser Leu Ala Cys Ala Asp Leu 
65                  70                  75                  80  


Val Met Gly Leu Ala Val Val Pro Phe Gly Ala Ala His Ile Leu Met 
                85                  90                  95      


Lys Met Trp Thr Phe Gly Asn Phe Trp Cys Glu Phe Trp Thr Ser Ile 
            100                 105                 110         


Asp Val Leu Cys Val Thr Ala Ser Ile Glu Thr Leu Cys Val Ile Ala 
        115                 120                 125             


Val Asp Arg Tyr Phe Ala Ile Thr Ser Pro Phe Lys Tyr Gln Ser Leu 
    130                 135                 140                 


Leu Thr Lys Asn Lys Ala Arg Val Ile Ile Leu Met Val Trp Ile Val 
145                 150                 155                 160 


Ser Gly Leu Thr Ser Phe Leu Pro Ile Gln Met His Trp Tyr Arg Ala 
                165                 170                 175     


Thr His Gln Glu Ala Ile Asn Cys Tyr Ala Asn Glu Thr Cys Cys Asp 
            180                 185                 190         


Phe Phe Thr Asn Gln Ala Tyr Ala Ile Ala Ser Ser Ile Val Ser Phe 
        195                 200                 205             


Tyr Val Pro Leu Val Ile Met Val Phe Val Tyr Ser Arg Val Phe Gln 
    210                 215                 220                 


Glu Ala Lys Arg Gln Leu Gln Lys Ile Asp Lys Ser Glu Gly Arg Phe 
225                 230                 235                 240 


His Val Gln Asn Leu Ser Gln Val Glu Gln Asp Gly Arg Thr Gly His 
                245                 250                 255     


Gly Leu Arg Arg Ser Ser Lys Phe Cys Leu Lys Glu His Lys Ala Leu 
            260                 265                 270         


Lys Thr Leu Gly Ile Ile Met Gly Thr Phe Thr Leu Cys Trp Leu Pro 
        275                 280                 285             


Phe Phe Ile Val Asn Ile Val His Val Ile Gln Asp Asn Leu Ile Arg 
    290                 295                 300                 


Lys Glu Val Tyr Ile Leu Leu Asn Trp Ile Gly Tyr Val Asn Ser Gly 
305                 310                 315                 320 


Phe Asn Pro Leu Ile Tyr Cys Arg Ser Pro Asp Phe Arg Ile Ala Phe 
                325                 330                 335     


Gln Glu Leu Leu Cys Leu Arg Arg Ser Ser Leu Lys Ala Tyr Gly Asn 
            340                 345                 350         


Gly Tyr Ser Ser Asn Gly Asn Thr Gly Glu Gln Ser Gly Tyr His Val 
        355                 360                 365             


Glu Gln Glu Lys Glu Asn Lys Leu Leu Cys Glu Asp Leu Pro Gly Thr 
    370                 375                 380                 


Glu Asp Phe Val Gly His Gln Gly Thr Val Pro Ser Asp Asn Ile Asp 
385                 390                 395                 400 


Ser Gln Gly Arg Asn Cys Ser Thr Asn Asp Ser Leu Leu 
                405                 410             


<210>  4
<211>  21
<212>  RNA
<213>  Artificial

<220>
<223>  siRNA

<400>  4
aaagccuucu gcgcggagaa u                                                 21


<210>  5
<211>  21
<212>  RNA
<213>  Artificial

<220>
<223>  siRNA

<400>  5
ggcuuguccu uccgcaaaga c                                                 21


<210>  6
<211>  23
<212>  RNA
<213>  Artificial

<220>
<223>  siRNA

<400>  6
aagaaguacg agaagcugga guu                                               23


<210>  7
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  offtarget siRNA

<400>  7
gataaggtgc tgcgtggacc c                                                 21


<210>  8
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  8
gaattccggc gcgtccgcct gtcgg                                             25


<210>  9
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  9
ctgcagttag tcgaagccgg ggcagg                                            26


<210>  10
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  10
gaattcgaga aagcaaagat aaatgc                                            26


<210>  11
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  11
ctgcagctac ttaaataact tgcagtcc                                          28


<210>  12
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  12
attgagaccc tgtgcgtgat                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  13
ctgaaagacc ctggagtaga                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  14
ttcctacccc caatgtgtcc                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  15
tcttgctcag tgtccttgct                                                   20


<210>  16
<211>  52
<212>  PRT
<213>  Neisseria meningitidis

<400>  16

Phe Thr Leu Ile Glu Leu Met Ile Val Ile Ala Ile Val Gly Ile Leu 
1               5                   10                  15      


Ala Ala Val Ala Leu Pro Ala Tyr Gln Asp Tyr Thr Ala Arg Ala Gln 
            20                  25                  30          


Val Ser Glu Ala Ile Leu Leu Ala Glu Gly Gln Lys Ser Ala Val Thr 
        35                  40                  45              


Glu Tyr Tyr Leu 
    50          


<210>  17
<211>  53
<212>  PRT
<213>  Neisseria meningitidis

<400>  17

Phe Thr Leu Leu Glu Leu Met Ile Ala Val Ala Ile Leu Gly Ile Leu 
1               5                   10                  15      


Thr Leu Ile Thr Tyr Pro Ser Tyr Lys Thr Tyr Ile Arg Arg Val Arg 
            20                  25                  30          


Leu Ser Glu Val Arg Thr Thr Leu Leu His Asn Ala Gln Thr Met Glu 
        35                  40                  45              


Arg Tyr Tyr Arg Gln 
    50              


<210>  18
<211>  117
<212>  PRT
<213>  Neisseria meningitidis

<400>  18

Ser Ala Val Thr Glu Tyr Tyr Leu Asn His Gly Glu Trp Pro Gly Asp 
1               5                   10                  15      


Asn Ser Ser Ala Gly Val Ala Thr Ser Ala Asp Ile Lys Gly Lys Tyr 
            20                  25                  30          


Val Gln Ser Val Thr Val Ala Asn Gly Val Ile Thr Ala Gln Met Ala 
        35                  40                  45              


Ser Ser Asn Val Asn Asn Glu Ile Lys Ser Lys Lys Leu Ser Leu Trp 
    50                  55                  60                  


Ala Lys Arg Gln Asn Gly Ser Val Lys Trp Phe Cys Gly Gln Pro Val 
65                  70                  75                  80  


Thr Arg Thr Thr Ala Thr Ala Thr Asp Val Ala Ala Ala Asn Gly Lys 
                85                  90                  95      


Thr Asp Asp Lys Ile Asn Thr Lys His Leu Pro Ser Thr Cys Arg Asp 
            100                 105                 110         


Asp Ser Ser Ala Ser 
        115         


<210>  19
<211>  76
<212>  PRT
<213>  Neisseria meningitidis

<400>  19

Thr Met Glu Arg Tyr Tyr Arg Gln Lys Gly Thr Phe Lys Thr Tyr Asp 
1               5                   10                  15      


Lys Asn Lys Leu Lys Gln Asn Lys Tyr Phe Asn Val Thr Leu Ser Lys 
            20                  25                  30          


Val Ser Pro Asp His Phe Thr Leu Gln Ala Asp Pro Asn Pro Thr Thr 
        35                  40                  45              


Asn Asp Gly Glu Thr Cys Val Val Thr Leu Asp Gly Gly Thr Ile Ala 
    50                  55                  60                  


Ala Ser Gly Thr Asn Gln Ser Cys Pro Gly Phe Asp 
65                  70                  75      


<210>  20
<211>  35
<212>  PRT
<213>  Neisseria meningitidis

<400>  20

Cys Gly Gln Pro Val Thr Arg Thr Thr Ala Thr Ala Thr Asp Val Ala 
1               5                   10                  15      


Ala Ala Asn Gly Lys Thr Asp Asp Lys Ile Asn Thr Lys His Leu Pro 
            20                  25                  30          


Ser Thr Cys 
        35  


<210>  21
<211>  27
<212>  PRT
<213>  Neisseria meningitidis

<400>  21

Gly Glu Thr Cys Val Val Thr Leu Asn Asp Gly Gly Thr Ile Ala Ala 
1               5                   10                  15      


Ser Gly Thr Asn Gln Ser Cys Pro Gly Phe Asp 
            20                  25          


<210>  22
<211>  280
<212>  PRT
<213>  Neisseria meningitidis

<400>  22

Met Thr Arg Ser Lys Pro Val Asn Arg Thr Ala Phe Cys Cys Leu Ser 
1               5                   10                  15      


Leu Thr Ala Ala Leu Ile Leu Thr Ala Cys Ser Ser Gly Gly Gly Gly 
            20                  25                  30          


Val Ala Ala Asp Ile Gly Ala Val Leu Ala Asp Ala Leu Thr Ala Pro 
        35                  40                  45              


Leu Asp His Lys Asp Lys Ser Leu Gln Ser Leu Thr Leu Asp Gln Ser 
    50                  55                  60                  


Val Arg Lys Asn Glu Lys Leu Lys Leu Ala Ala Gln Gly Ala Glu Lys 
65                  70                  75                  80  


Thr Tyr Gly Asn Gly Asp Ser Leu Asn Thr Gly Lys Leu Lys Asn Asp 
                85                  90                  95      


Lys Val Ser Arg Phe Asp Phe Ile Arg Gln Ile Glu Val Asp Gly Gln 
            100                 105                 110         


Leu Ile Thr Leu Glu Ser Gly Glu Phe Gln Val Tyr Lys Gln Ser His 
        115                 120                 125             


Ser Ala Leu Thr Ala Leu Gln Thr Glu Gln Val Gln Asp Ser Glu His 
    130                 135                 140                 


Ser Gly Lys Met Val Ala Lys Arg Gln Phe Arg Ile Gly Asp Ile Ala 
145                 150                 155                 160 


Gly Glu His Thr Ser Phe Asp Lys Leu Pro Glu Gly Gly Arg Ala Thr 
                165                 170                 175     


Tyr Arg Gly Thr Ala Phe Gly Ser Asp Asp Ala Ser Gly Lys Leu Thr 
            180                 185                 190         


Tyr Thr Ile Asp Phe Ala Ala Lys Gln Gly His Gly Lys Ile Glu His 
        195                 200                 205             


Leu Lys Ser Pro Glu Leu Asn Val Asp Leu Ala Ala Ser Asp Ile Lys 
    210                 215                 220                 


Pro Asp Lys Lys Arg His Ala Val Ile Ser Gly Ser Val Leu Tyr Asn 
225                 230                 235                 240 


Gln Ala Glu Lys Gly Ser Tyr Ser Leu Gly Ile Phe Gly Gly Gln Ala 
                245                 250                 255     


Gln Glu Val Ala Gly Ser Ala Glu Val Glu Thr Ala Asn Gly Ile Arg 
            260                 265                 270         


His Ile Gly Leu Ala Ala Lys Gln 
        275                 280 


<210>  23
<211>  279
<212>  PRT
<213>  Neisseria meningitidis

<400>  23

Met Thr Arg Ser Lys Pro Val Asn Arg Thr Ala Phe Cys Cys Leu Ser 
1               5                   10                  15      


Leu Thr Ala Ala Leu Ile Leu Thr Ala Cys Ser Ser Gly Gly Gly Gly 
            20                  25                  30          


Val Ala Ala Asp Ile Gly Ala Gly Leu Ala Asp Ala Leu Thr Ala Pro 
        35                  40                  45              


Leu Asp His Lys Asp Lys Ser Leu Gln Ser Leu Thr Leu Asp Gln Ser 
    50                  55                  60                  


Val Arg Lys Asn Glu Lys Leu Lys Leu Ala Ala Gln Gly Ala Glu Lys 
65                  70                  75                  80  


Thr Tyr Gly Asn Gly Asp Ser Leu Asn Thr Gly Lys Leu Lys Asn Asp 
                85                  90                  95      


Lys Val Ser Arg Phe Asp Phe Ile Arg Gln Ile Glu Val Asp Gly Gln 
            100                 105                 110         


Leu Ile Thr Leu Glu Ser Gly Glu Phe Gln Ile Tyr Lys Gln Asp His 
        115                 120                 125             


Ser Ala Val Val Ala Leu Gln Ile Glu Lys Ile Asn Asn Pro Asp Lys 
    130                 135                 140                 


Ile Asp Ser Leu Ile Asn Gln Arg Ser Phe Leu Val Ser Gly Leu Gly 
145                 150                 155                 160 


Gly Glu His Thr Ala Phe Asn Gln Leu Pro Gly Gly Lys Ala Glu Tyr 
                165                 170                 175     


His Gly Lys Ala Phe Ser Ser Asp Asp Ala Gly Gly Lys Leu Thr Tyr 
            180                 185                 190         


Thr Ile Asp Phe Ala Ala Lys Gln Gly His Gly Lys Ile Glu His Leu 
        195                 200                 205             


Lys Thr Pro Glu Gln Asn Val Glu Leu Ala Ala Ala Glu Leu Lys Ala 
    210                 215                 220                 


Asp Glu Lys Ser His Ala Val Ile Leu Gly Asp Thr Arg Tyr Gly Ser 
225                 230                 235                 240 


Glu Glu Lys Gly Thr Tyr His Leu Ala Leu Phe Gly Asp Arg Ala Gln 
                245                 250                 255     


Glu Ile Ala Gly Ser Ala Thr Val Lys Ile Gly Glu Lys Val His Glu 
            260                 265                 270         


Ile Gly Ile Ala Gly Lys Gln 
        275                 


<210>  24
<211>  395
<212>  PRT
<213>  Neisseria meningitidis

<400>  24

Met Arg Lys Lys Leu Thr Ala Leu Val Leu Ser Ala Leu Pro Leu Ala 
1               5                   10                  15      


Ala Val Ala Asp Val Ser Leu Tyr Gly Glu Ile Lys Ala Gly Val Glu 
            20                  25                  30          


Gly Arg Asn Tyr Gln Leu Gln Leu Thr Glu Ala Gln Ala Ala Asn Gly 
        35                  40                  45              


Gly Ala Ser Gly Gln Val Lys Val Thr Lys Val Thr Lys Ala Lys Ser 
    50                  55                  60                  


Arg Ile Arg Thr Lys Ile Ser Asp Phe Gly Ser Phe Ile Gly Phe Lys 
65                  70                  75                  80  


Gly Ser Glu Asp Leu Gly Glu Gly Leu Lys Ala Val Trp Gln Leu Glu 
                85                  90                  95      


Gln Asp Val Ser Val Ala Gly Gly Gly Ala Thr Gln Trp Gly Asn Arg 
            100                 105                 110         


Glu Ser Phe Ile Gly Leu Ala Gly Glu Phe Gly Thr Leu Arg Ala Gly 
        115                 120                 125             


Arg Val Ala Asn Gln Phe Asp Asp Ala Ser Gln Ala Ile Asp Pro Trp 
    130                 135                 140                 


Asp Ser Asn Asn Asp Val Ala Ser Gln Leu Gly Ile Phe Lys Arg His 
145                 150                 155                 160 


Asp Asp Met Pro Val Ser Val Arg Tyr Asp Ser Pro Glu Phe Ser Gly 
                165                 170                 175     


Phe Ser Gly Ser Val Gln Phe Val Pro Ala Gln Asn Ser Lys Ser Ala 
            180                 185                 190         


Tyr Lys Pro Ala Tyr Trp Thr Thr Val Asn Thr Gly Ser Ala Thr Thr 
        195                 200                 205             


Thr Thr Phe Val Pro Ala Val Val Gly Lys Pro Gly Ser Asp Val Tyr 
    210                 215                 220                 


Tyr Ala Gly Leu Asn Tyr Lys Asn Gly Gly Phe Ala Gly Asn Tyr Ala 
225                 230                 235                 240 


Phe Lys Tyr Ala Arg His Ala Asn Val Gly Arg Asp Ala Phe Glu Leu 
                245                 250                 255     


Phe Leu Leu Gly Ser Gly Ser Asp Gln Ala Lys Gly Thr Asp Pro Leu 
            260                 265                 270         


Lys Asn His Gln Val His Arg Leu Thr Gly Gly Tyr Glu Glu Gly Gly 
        275                 280                 285             


Leu Asn Leu Ala Leu Ala Ala Gln Leu Asp Leu Ser Glu Asn Gly Asp 
    290                 295                 300                 


Lys Thr Lys Asn Ser Thr Thr Glu Ile Ala Ala Thr Ala Ser Tyr Arg 
305                 310                 315                 320 


Phe Gly Asn Ala Val Pro Arg Ile Ser Tyr Ala His Gly Phe Asp Phe 
                325                 330                 335     


Ile Glu Arg Gly Lys Lys Gly Glu Asn Thr Ser Tyr Asp Gln Ile Ile 
            340                 345                 350         


Ala Gly Val Asp Tyr Asp Phe Ser Lys Arg Thr Ser Ala Ile Val Ser 
        355                 360                 365             


Gly Ala Trp Leu Lys Arg Asn Thr Gly Ile Gly Asn Tyr Thr Gln Ile 
    370                 375                 380                 


Asn Ala Ala Ser Val Gly Leu Arg His Lys Phe 
385                 390                 395 


<210>  25
<211>  497
<212>  PRT
<213>  Neisseria meningitidis

<400>  25

Met Phe Lys Arg Ser Val Ile Ala Met Ala Cys Ile Val Ala Leu Ser 
1               5                   10                  15      


Ala Cys Gly Gly Gly Gly Gly Gly Ser Pro Asp Val Lys Ser Ala Asp 
            20                  25                  30          


Thr Leu Ser Lys Pro Ala Ala Pro Val Val Thr Glu Asp Val Gly Glu 
        35                  40                  45              


Glu Val Leu Pro Lys Glu Lys Lys Asp Glu Glu Ala Val Ser Gly Ala 
    50                  55                  60                  


Pro Gln Ala Asp Thr Gln Asp Ala Thr Ala Gly Lys Gly Gly Gln Asp 
65                  70                  75                  80  


Met Ala Ala Val Ser Ala Glu Asn Thr Gly Asn Gly Gly Ala Ala Thr 
                85                  90                  95      


Thr Asp Asn Pro Glu Asn Lys Asp Glu Gly Pro Gln Asn Asp Met Pro 
            100                 105                 110         


Gln Asn Ala Ala Asp Thr Asp Ser Ser Thr Pro Asn His Thr Pro Ala 
        115                 120                 125             


Pro Asn Met Pro Thr Arg Asp Met Gly Asn Gln Ala Pro Asp Ala Gly 
    130                 135                 140                 


Glu Ser Ala Gln Pro Ala Asn Gln Pro Asp Met Ala Asn Ala Ala Asp 
145                 150                 155                 160 


Gly Met Gln Gly Asp Asp Pro Ser Ala Gly Glu Asn Ala Gly Asn Thr 
                165                 170                 175     


Ala Asp Gln Ala Ala Asn Gln Ala Glu Asn Asn Gln Val Gly Gly Ser 
            180                 185                 190         


Gln Asn Pro Ala Ser Ser Thr Asn Pro Asn Ala Thr Asn Gly Gly Ser 
        195                 200                 205             


Asp Phe Gly Arg Ile Asn Val Ala Asn Gly Ile Lys Leu Asp Ser Gly 
    210                 215                 220                 


Ser Glu Asn Val Thr Leu Thr His Cys Lys Asp Lys Val Cys Asp Arg 
225                 230                 235                 240 


Asp Phe Leu Asp Glu Glu Ala Pro Pro Lys Ser Glu Phe Glu Lys Leu 
                245                 250                 255     


Ser Asp Glu Glu Lys Ile Asn Lys Tyr Lys Lys Asp Glu Gln Arg Glu 
            260                 265                 270         


Asn Phe Val Gly Leu Val Ala Asp Arg Val Glu Lys Asn Gly Thr Asn 
        275                 280                 285             


Lys Tyr Val Ile Ile Tyr Lys Asp Lys Ser Ala Ser Ser Ser Ser Ala 
    290                 295                 300                 


Arg Phe Arg Arg Ser Ala Arg Ser Arg Arg Ser Leu Pro Ala Glu Met 
305                 310                 315                 320 


Pro Leu Ile Pro Val Asn Gln Ala Asp Thr Leu Ile Val Asp Gly Glu 
                325                 330                 335     


Ala Val Ser Leu Thr Gly His Ser Gly Asn Ile Phe Ala Pro Glu Gly 
            340                 345                 350         


Asn Tyr Arg Tyr Leu Thr Tyr Gly Ala Glu Lys Leu Ser Gly Gly Ser 
        355                 360                 365             


Tyr Ala Leu Ser Val Gln Gly Glu Pro Ala Lys Gly Glu Met Leu Ala 
    370                 375                 380                 


Gly Thr Ala Val Tyr Asn Gly Glu Val Leu His Phe His Met Glu Asn 
385                 390                 395                 400 


Gly Arg Pro Ser Pro Ser Gly Gly Arg Phe Ala Ala Lys Val Asp Phe 
                405                 410                 415     


Gly Ser Lys Ser Val Asp Gly Ile Ile Asp Ser Gly Asp Asp Leu His 
            420                 425                 430         


Met Gly Thr Gln Lys Phe Lys Ala Val Ile Asp Gly Asn Gly Phe Lys 
        435                 440                 445             


Gly Thr Trp Thr Glu Asn Gly Gly Gly Asp Val Ser Gly Arg Phe Tyr 
    450                 455                 460                 


Gly Pro Ala Gly Glu Glu Val Ala Gly Lys Tyr Ser Tyr Arg Pro Thr 
465                 470                 475                 480 


Asp Ala Glu Lys Gly Gly Phe Gly Val Phe Ala Gly Lys Lys Glu Gln 
                485                 490                 495     


Asp 
    


<210>  26
<211>  364
<212>  PRT
<213>  Neisseria meningitidis

<400>  26

Met Ser Met Lys His Phe Pro Ser Lys Val Leu Thr Thr Ala Ile Leu 
1               5                   10                  15      


Ala Thr Phe Cys Ser Gly Ala Leu Ala Ala Thr Ser Asp Asp Asp Val 
            20                  25                  30          


Lys Lys Ala Ala Thr Val Ala Ile Val Ala Ala Tyr Asn Asn Gly Gln 
        35                  40                  45              


Glu Ile Asn Gly Phe Lys Ala Gly Glu Thr Ile Tyr Asp Ile Gly Glu 
    50                  55                  60                  


Asp Gly Thr Ile Thr Gln Lys Asp Ala Thr Ala Ala Asp Val Glu Ala 
65                  70                  75                  80  


Asp Asp Phe Lys Gly Leu Gly Leu Lys Lys Val Val Thr Asn Leu Thr 
                85                  90                  95      


Lys Thr Val Asn Glu Asn Lys Gln Asn Val Asp Ala Lys Val Lys Ala 
            100                 105                 110         


Ala Glu Ser Glu Ile Glu Lys Leu Thr Thr Lys Leu Ala Asp Thr Asp 
        115                 120                 125             


Ala Ala Leu Ala Asp Thr Asp Ala Ala Leu Asp Glu Thr Thr Asn Ala 
    130                 135                 140                 


Leu Asn Lys Leu Gly Glu Asn Ile Thr Thr Phe Ala Glu Glu Thr Lys 
145                 150                 155                 160 


Thr Asn Ile Val Lys Ile Asp Glu Lys Leu Glu Ala Val Ala Asp Thr 
                165                 170                 175     


Val Asp Lys His Ala Glu Ala Phe Asn Asp Ile Ala Asp Ser Leu Asp 
            180                 185                 190         


Glu Thr Asn Thr Lys Ala Asp Glu Ala Val Lys Thr Ala Asn Glu Ala 
        195                 200                 205             


Lys Gln Thr Ala Glu Glu Thr Lys Gln Asn Val Asp Ala Lys Val Lys 
    210                 215                 220                 


Ala Ala Glu Thr Ala Ala Gly Lys Ala Glu Ala Ala Ala Gly Thr Ala 
225                 230                 235                 240 


Asn Thr Ala Ala Asp Lys Ala Glu Ala Val Ala Ala Lys Val Thr Asp 
                245                 250                 255     


Ile Lys Ala Asp Ile Ala Thr Asn Lys Ala Asp Ile Ala Lys Asn Ser 
            260                 265                 270         


Ala Arg Ile Asp Ser Leu Asp Lys Asn Val Ala Asn Leu Arg Lys Glu 
        275                 280                 285             


Thr Arg Gln Gly Leu Ala Glu Gln Ala Ala Leu Ser Gly Leu Phe Gln 
    290                 295                 300                 


Pro Tyr Asn Val Gly Arg Phe Asn Val Thr Ala Ala Val Gly Gly Tyr 
305                 310                 315                 320 


Lys Ser Glu Ser Ala Val Ala Ile Gly Thr Gly Phe Arg Phe Thr Glu 
                325                 330                 335     


Asn Phe Ala Ala Lys Ala Gly Val Ala Val Gly Thr Ser Ser Gly Ser 
            340                 345                 350         


Ser Ala Ala Tyr His Val Gly Val Asn Tyr Glu Trp 
        355                 360                 


<210>  27
<211>  396
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein PilE-fHBP

<400>  27

Ser Ala Val Thr Glu Tyr Tyr Leu Asn His Gly Glu Trp Pro Gly Asp 
1               5                   10                  15      


Asn Ser Ser Ala Gly Val Ala Thr Ser Ala Asp Ile Lys Gly Lys Tyr 
            20                  25                  30          


Val Gln Ser Val Thr Val Ala Asn Gly Val Ile Thr Ala Gln Met Ala 
        35                  40                  45              


Ser Ser Asn Val Asn Asn Glu Ile Lys Ser Lys Lys Leu Ser Leu Trp 
    50                  55                  60                  


Ala Lys Arg Gln Asn Gly Ser Val Lys Trp Phe Cys Gly Gln Pro Val 
65                  70                  75                  80  


Thr Arg Thr Thr Ala Thr Ala Thr Asp Val Ala Ala Ala Asn Gly Lys 
                85                  90                  95      


Thr Asp Asp Lys Ile Asn Thr Lys His Leu Pro Ser Thr Cys Arg Asp 
            100                 105                 110         


Asp Ser Ser Ala Ser Met Thr Arg Ser Lys Pro Val Asn Arg Thr Ala 
        115                 120                 125             


Phe Cys Cys Leu Ser Leu Thr Ala Ala Leu Ile Leu Thr Ala Cys Ser 
    130                 135                 140                 


Ser Gly Gly Gly Gly Val Ala Ala Asp Ile Gly Ala Gly Leu Ala Asp 
145                 150                 155                 160 


Ala Leu Thr Ala Pro Leu Asp His Lys Asp Lys Ser Leu Gln Ser Leu 
                165                 170                 175     


Thr Leu Asp Gln Ser Val Arg Lys Asn Glu Lys Leu Lys Leu Ala Ala 
            180                 185                 190         


Gln Gly Ala Glu Lys Thr Tyr Gly Asn Gly Asp Ser Leu Asn Thr Gly 
        195                 200                 205             


Lys Leu Lys Asn Asp Lys Val Ser Arg Phe Asp Phe Ile Arg Gln Ile 
    210                 215                 220                 


Glu Val Asp Gly Gln Leu Ile Thr Leu Glu Ser Gly Glu Phe Gln Ile 
225                 230                 235                 240 


Tyr Lys Gln Asp His Ser Ala Val Val Ala Leu Gln Ile Glu Lys Ile 
                245                 250                 255     


Asn Asn Pro Asp Lys Ile Asp Ser Leu Ile Asn Gln Arg Ser Phe Leu 
            260                 265                 270         


Val Ser Gly Leu Gly Gly Glu His Thr Ala Phe Asn Gln Leu Pro Gly 
        275                 280                 285             


Gly Lys Ala Glu Tyr His Gly Lys Ala Phe Ser Ser Asp Asp Ala Gly 
    290                 295                 300                 


Gly Lys Leu Thr Tyr Thr Ile Asp Phe Ala Ala Lys Gln Gly His Gly 
305                 310                 315                 320 


Lys Ile Glu His Leu Lys Thr Pro Glu Gln Asn Val Glu Leu Ala Ala 
                325                 330                 335     


Ala Glu Leu Lys Ala Asp Glu Lys Ser His Ala Val Ile Leu Gly Asp 
            340                 345                 350         


Thr Arg Tyr Gly Ser Glu Glu Lys Gly Thr Tyr His Leu Ala Leu Phe 
        355                 360                 365             


Gly Asp Arg Ala Gln Glu Ile Ala Gly Ser Ala Thr Val Lys Ile Gly 
    370                 375                 380                 


Glu Lys Val His Glu Ile Gly Ile Ala Gly Lys Gln 
385                 390                 395     


<210>  28
<211>  314
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein PilE-fHBP

<400>  28

Cys Gly Gln Pro Val Thr Arg Thr Thr Ala Thr Ala Thr Asp Val Ala 
1               5                   10                  15      


Ala Ala Asn Gly Lys Thr Asp Asp Lys Ile Asn Thr Lys His Leu Pro 
            20                  25                  30          


Ser Thr Cys Met Thr Arg Ser Lys Pro Val Asn Arg Thr Ala Phe Cys 
        35                  40                  45              


Cys Leu Ser Leu Thr Ala Ala Leu Ile Leu Thr Ala Cys Ser Ser Gly 
    50                  55                  60                  


Gly Gly Gly Val Ala Ala Asp Ile Gly Ala Gly Leu Ala Asp Ala Leu 
65                  70                  75                  80  


Thr Ala Pro Leu Asp His Lys Asp Lys Ser Leu Gln Ser Leu Thr Leu 
                85                  90                  95      


Asp Gln Ser Val Arg Lys Asn Glu Lys Leu Lys Leu Ala Ala Gln Gly 
            100                 105                 110         


Ala Glu Lys Thr Tyr Gly Asn Gly Asp Ser Leu Asn Thr Gly Lys Leu 
        115                 120                 125             


Lys Asn Asp Lys Val Ser Arg Phe Asp Phe Ile Arg Gln Ile Glu Val 
    130                 135                 140                 


Asp Gly Gln Leu Ile Thr Leu Glu Ser Gly Glu Phe Gln Ile Tyr Lys 
145                 150                 155                 160 


Gln Asp His Ser Ala Val Val Ala Leu Gln Ile Glu Lys Ile Asn Asn 
                165                 170                 175     


Pro Asp Lys Ile Asp Ser Leu Ile Asn Gln Arg Ser Phe Leu Val Ser 
            180                 185                 190         


Gly Leu Gly Gly Glu His Thr Ala Phe Asn Gln Leu Pro Gly Gly Lys 
        195                 200                 205             


Ala Glu Tyr His Gly Lys Ala Phe Ser Ser Asp Asp Ala Gly Gly Lys 
    210                 215                 220                 


Leu Thr Tyr Thr Ile Asp Phe Ala Ala Lys Gln Gly His Gly Lys Ile 
225                 230                 235                 240 


Glu His Leu Lys Thr Pro Glu Gln Asn Val Glu Leu Ala Ala Ala Glu 
                245                 250                 255     


Leu Lys Ala Asp Glu Lys Ser His Ala Val Ile Leu Gly Asp Thr Arg 
            260                 265                 270         


Tyr Gly Ser Glu Glu Lys Gly Thr Tyr His Leu Ala Leu Phe Gly Asp 
        275                 280                 285             


Arg Ala Gln Glu Ile Ala Gly Ser Ala Thr Val Lys Ile Gly Glu Lys 
    290                 295                 300                 


Val His Glu Ile Gly Ile Ala Gly Lys Gln 
305                 310                 


<210>  29
<211>  355
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein PilV-fHBP

<400>  29

Thr Met Glu Arg Tyr Tyr Arg Gln Lys Gly Thr Phe Lys Thr Tyr Asp 
1               5                   10                  15      


Lys Asn Lys Leu Lys Gln Asn Lys Tyr Phe Asn Val Thr Leu Ser Lys 
            20                  25                  30          


Val Ser Pro Asp His Phe Thr Leu Gln Ala Asp Pro Asn Pro Thr Thr 
        35                  40                  45              


Asn Asp Gly Glu Thr Cys Val Val Thr Leu Asp Gly Gly Thr Ile Ala 
    50                  55                  60                  


Ala Ser Gly Thr Asn Gln Ser Cys Pro Gly Phe Asp Met Thr Arg Ser 
65                  70                  75                  80  


Lys Pro Val Asn Arg Thr Ala Phe Cys Cys Leu Ser Leu Thr Ala Ala 
                85                  90                  95      


Leu Ile Leu Thr Ala Cys Ser Ser Gly Gly Gly Gly Val Ala Ala Asp 
            100                 105                 110         


Ile Gly Ala Gly Leu Ala Asp Ala Leu Thr Ala Pro Leu Asp His Lys 
        115                 120                 125             


Asp Lys Ser Leu Gln Ser Leu Thr Leu Asp Gln Ser Val Arg Lys Asn 
    130                 135                 140                 


Glu Lys Leu Lys Leu Ala Ala Gln Gly Ala Glu Lys Thr Tyr Gly Asn 
145                 150                 155                 160 


Gly Asp Ser Leu Asn Thr Gly Lys Leu Lys Asn Asp Lys Val Ser Arg 
                165                 170                 175     


Phe Asp Phe Ile Arg Gln Ile Glu Val Asp Gly Gln Leu Ile Thr Leu 
            180                 185                 190         


Glu Ser Gly Glu Phe Gln Ile Tyr Lys Gln Asp His Ser Ala Val Val 
        195                 200                 205             


Ala Leu Gln Ile Glu Lys Ile Asn Asn Pro Asp Lys Ile Asp Ser Leu 
    210                 215                 220                 


Ile Asn Gln Arg Ser Phe Leu Val Ser Gly Leu Gly Gly Glu His Thr 
225                 230                 235                 240 


Ala Phe Asn Gln Leu Pro Gly Gly Lys Ala Glu Tyr His Gly Lys Ala 
                245                 250                 255     


Phe Ser Ser Asp Asp Ala Gly Gly Lys Leu Thr Tyr Thr Ile Asp Phe 
            260                 265                 270         


Ala Ala Lys Gln Gly His Gly Lys Ile Glu His Leu Lys Thr Pro Glu 
        275                 280                 285             


Gln Asn Val Glu Leu Ala Ala Ala Glu Leu Lys Ala Asp Glu Lys Ser 
    290                 295                 300                 


His Ala Val Ile Leu Gly Asp Thr Arg Tyr Gly Ser Glu Glu Lys Gly 
305                 310                 315                 320 


Thr Tyr His Leu Ala Leu Phe Gly Asp Arg Ala Gln Glu Ile Ala Gly 
                325                 330                 335     


Ser Ala Thr Val Lys Ile Gly Glu Lys Val His Glu Ile Gly Ile Ala 
            340                 345                 350         


Gly Lys Gln 
        355 


<210>  30
<211>  306
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein PilV-fHBP

<400>  30

Gly Glu Thr Cys Val Val Thr Leu Asn Asp Gly Gly Thr Ile Ala Ala 
1               5                   10                  15      


Ser Gly Thr Asn Gln Ser Cys Pro Gly Phe Asp Met Thr Arg Ser Lys 
            20                  25                  30          


Pro Val Asn Arg Thr Ala Phe Cys Cys Leu Ser Leu Thr Ala Ala Leu 
        35                  40                  45              


Ile Leu Thr Ala Cys Ser Ser Gly Gly Gly Gly Val Ala Ala Asp Ile 
    50                  55                  60                  


Gly Ala Gly Leu Ala Asp Ala Leu Thr Ala Pro Leu Asp His Lys Asp 
65                  70                  75                  80  


Lys Ser Leu Gln Ser Leu Thr Leu Asp Gln Ser Val Arg Lys Asn Glu 
                85                  90                  95      


Lys Leu Lys Leu Ala Ala Gln Gly Ala Glu Lys Thr Tyr Gly Asn Gly 
            100                 105                 110         


Asp Ser Leu Asn Thr Gly Lys Leu Lys Asn Asp Lys Val Ser Arg Phe 
        115                 120                 125             


Asp Phe Ile Arg Gln Ile Glu Val Asp Gly Gln Leu Ile Thr Leu Glu 
    130                 135                 140                 


Ser Gly Glu Phe Gln Ile Tyr Lys Gln Asp His Ser Ala Val Val Ala 
145                 150                 155                 160 


Leu Gln Ile Glu Lys Ile Asn Asn Pro Asp Lys Ile Asp Ser Leu Ile 
                165                 170                 175     


Asn Gln Arg Ser Phe Leu Val Ser Gly Leu Gly Gly Glu His Thr Ala 
            180                 185                 190         


Phe Asn Gln Leu Pro Gly Gly Lys Ala Glu Tyr His Gly Lys Ala Phe 
        195                 200                 205             


Ser Ser Asp Asp Ala Gly Gly Lys Leu Thr Tyr Thr Ile Asp Phe Ala 
    210                 215                 220                 


Ala Lys Gln Gly His Gly Lys Ile Glu His Leu Lys Thr Pro Glu Gln 
225                 230                 235                 240 


Asn Val Glu Leu Ala Ala Ala Glu Leu Lys Ala Asp Glu Lys Ser His 
                245                 250                 255     


Ala Val Ile Leu Gly Asp Thr Arg Tyr Gly Ser Glu Glu Lys Gly Thr 
            260                 265                 270         


Tyr His Leu Ala Leu Phe Gly Asp Arg Ala Gln Glu Ile Ala Gly Ser 
        275                 280                 285             


Ala Thr Val Lys Ile Gly Glu Lys Val His Glu Ile Gly Ile Ala Gly 
    290                 295                 300                 


Lys Gln 
305     


