                         SEQUENCE LISTING

<110>  ZYMEWORKS INC.
       CABRERA, Eric
       von KREUDENSTEIN, Thomas
       DIXIT, Surjit
       LARIO, Paula
       POON, David
       D'ANGELO, Igor
 
<120>  Stable Heterodimeric Antibody Design with Mutations in the Fc 
       Domain

<130>  V84467WO

<150>  US 61/410,746
<151>  2010-11-05

<150>  US 61/425,375
<151>  2010-12-21

<150>  US 61/439,341
<151>  2011-02-03

<150>  US 61/475,614
<151>  2011-04-14

<150>  US 61/491,846
<151>  2011-05-31

<150>  US 61/497,861
<151>  2011-06-16

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  212
<212>  PRT
<213>  Homo sapiens

<400>  1

Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
1               5                   10                  15      


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
            20                  25                  30          


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
        35                  40                  45              


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
    50                  55                  60                  


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
65                  70                  75                  80  


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
                85                  90                  95      


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
            100                 105                 110         


Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
        115                 120                 125             


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
    130                 135                 140                 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
145                 150                 155                 160 


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
                165                 170                 175     


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
            180                 185                 190         


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
        195                 200                 205             


Ser Pro Gly Lys 
    210         


<210>  2
<211>  317
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Thr Met Glu Thr Gln Met Ser Gln Asn Val Cys Pro Arg Asn Leu 
1               5                   10                  15      


Trp Leu Leu Gln Pro Leu Thr Val Leu Leu Leu Leu Ala Ser Ala Asp 
            20                  25                  30          


Ser Gln Ala Ala Ala Pro Pro Lys Ala Val Leu Lys Leu Glu Pro Pro 
        35                  40                  45              


Trp Ile Asn Val Leu Gln Glu Asp Ser Val Thr Leu Thr Cys Gln Gly 
    50                  55                  60                  


Ala Arg Ser Pro Glu Ser Asp Ser Ile Gln Trp Phe His Asn Gly Asn 
65                  70                  75                  80  


Leu Ile Pro Thr His Thr Gln Pro Ser Tyr Arg Phe Lys Ala Asn Asn 
                85                  90                  95      


Asn Asp Ser Gly Glu Tyr Thr Cys Gln Thr Gly Gln Thr Ser Leu Ser 
            100                 105                 110         


Asp Pro Val His Leu Thr Val Leu Ser Glu Trp Leu Val Leu Gln Thr 
        115                 120                 125             


Pro His Leu Glu Phe Gln Glu Gly Glu Thr Ile Met Leu Arg Cys His 
    130                 135                 140                 


Ser Trp Lys Asp Lys Pro Leu Val Lys Val Thr Phe Phe Gln Asn Gly 
145                 150                 155                 160 


Lys Ser Gln Lys Phe Ser His Leu Asp Pro Thr Phe Ser Ile Pro Gln 
                165                 170                 175     


Ala Asn His Ser His Ser Gly Asp Tyr His Cys Thr Gly Asn Ile Gly 
            180                 185                 190         


Tyr Thr Leu Phe Ser Ser Lys Pro Val Thr Ile Thr Val Gln Val Pro 
        195                 200                 205             


Ser Met Gly Ser Ser Ser Pro Met Gly Ile Ile Val Ala Val Val Ile 
    210                 215                 220                 


Ala Thr Ala Val Ala Ala Ile Val Ala Ala Val Val Ala Leu Ile Tyr 
225                 230                 235                 240 


Cys Arg Lys Lys Arg Ile Ser Ala Asn Ser Thr Asp Pro Val Lys Ala 
                245                 250                 255     


Ala Gln Phe Glu Pro Pro Gly Arg Gln Met Ile Ala Ile Arg Lys Arg 
            260                 265                 270         


Gln Leu Glu Glu Thr Asn Asn Asp Tyr Glu Thr Ala Asp Gly Gly Tyr 
        275                 280                 285             


Met Thr Leu Asn Pro Arg Ala Pro Thr Asp Asp Asp Lys Asn Ile Tyr 
    290                 295                 300                 


Leu Thr Leu Pro Pro Asn Asp His Val Asn Ser Asn Asn 
305                 310                 315         


<210>  3
<211>  310
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Gly Ile Leu Ser Phe Leu Pro Val Leu Ala Thr Glu Ser Asp Trp 
1               5                   10                  15      


Ala Asp Cys Lys Ser Pro Gln Pro Trp Gly His Met Leu Leu Trp Thr 
            20                  25                  30          


Ala Val Leu Phe Leu Ala Pro Val Ala Gly Thr Pro Ala Ala Pro Pro 
        35                  40                  45              


Lys Ala Val Leu Lys Leu Glu Pro Gln Trp Ile Asn Val Leu Gln Glu 
    50                  55                  60                  


Asp Ser Val Thr Leu Thr Cys Arg Gly Thr His Ser Pro Glu Ser Asp 
65                  70                  75                  80  


Ser Ile Gln Trp Phe His Asn Gly Asn Leu Ile Pro Thr His Thr Gln 
                85                  90                  95      


Pro Ser Tyr Arg Phe Lys Ala Asn Asn Asn Asp Ser Gly Glu Tyr Thr 
            100                 105                 110         


Cys Gln Thr Gly Gln Thr Ser Leu Ser Asp Pro Val His Leu Thr Val 
        115                 120                 125             


Leu Ser Glu Trp Leu Val Leu Gln Thr Pro His Leu Glu Phe Gln Glu 
    130                 135                 140                 


Gly Glu Thr Ile Val Leu Arg Cys His Ser Trp Lys Asp Lys Pro Leu 
145                 150                 155                 160 


Val Lys Val Thr Phe Phe Gln Asn Gly Lys Ser Lys Lys Phe Ser Arg 
                165                 170                 175     


Ser Asp Pro Asn Phe Ser Ile Pro Gln Ala Asn His Ser His Ser Gly 
            180                 185                 190         


Asp Tyr His Cys Thr Gly Asn Ile Gly Tyr Thr Leu Tyr Ser Ser Lys 
        195                 200                 205             


Pro Val Thr Ile Thr Val Gln Ala Pro Ser Ser Ser Pro Met Gly Ile 
    210                 215                 220                 


Ile Val Ala Val Val Thr Gly Ile Ala Val Ala Ala Ile Val Ala Ala 
225                 230                 235                 240 


Val Val Ala Leu Ile Tyr Cys Arg Lys Lys Arg Ile Ser Ala Leu Pro 
                245                 250                 255     


Gly Tyr Pro Glu Cys Arg Glu Met Gly Glu Thr Leu Pro Glu Lys Pro 
            260                 265                 270         


Ala Asn Pro Thr Asn Pro Asp Glu Ala Asp Lys Val Gly Ala Glu Asn 
        275                 280                 285             


Thr Ile Thr Tyr Ser Leu Leu Met His Pro Asp Ala Leu Glu Glu Pro 
    290                 295                 300                 


Asp Asp Gln Asn Arg Ile 
305                 310 


<210>  4
<211>  323
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Gly Ile Leu Ser Phe Leu Pro Val Leu Ala Thr Glu Ser Asp Trp 
1               5                   10                  15      


Ala Asp Cys Lys Ser Pro Gln Pro Trp Gly His Met Leu Leu Trp Thr 
            20                  25                  30          


Ala Val Leu Phe Leu Ala Pro Val Ala Gly Thr Pro Ala Ala Pro Pro 
        35                  40                  45              


Lys Ala Val Leu Lys Leu Glu Pro Gln Trp Ile Asn Val Leu Gln Glu 
    50                  55                  60                  


Asp Ser Val Thr Leu Thr Cys Arg Gly Thr His Ser Pro Glu Ser Asp 
65                  70                  75                  80  


Ser Ile Pro Trp Phe His Asn Gly Asn Leu Ile Pro Thr His Thr Gln 
                85                  90                  95      


Pro Ser Tyr Arg Phe Lys Ala Asn Asn Asn Asp Ser Gly Glu Tyr Thr 
            100                 105                 110         


Cys Gln Thr Gly Gln Thr Ser Leu Ser Asp Pro Val His Leu Thr Val 
        115                 120                 125             


Leu Ser Glu Trp Leu Val Leu Gln Thr Pro His Leu Glu Phe Gln Glu 
    130                 135                 140                 


Gly Glu Thr Ile Val Leu Arg Cys His Ser Trp Lys Asp Lys Pro Leu 
145                 150                 155                 160 


Val Lys Val Thr Phe Phe Gln Asn Gly Lys Ser Lys Lys Phe Ser Arg 
                165                 170                 175     


Ser Asp Pro Asn Phe Ser Ile Pro Gln Ala Asn His Ser His Ser Gly 
            180                 185                 190         


Asp Tyr His Cys Thr Gly Asn Ile Gly Tyr Thr Leu Tyr Ser Ser Lys 
        195                 200                 205             


Pro Val Thr Ile Thr Val Gln Ala Pro Ser Ser Ser Pro Met Gly Ile 
    210                 215                 220                 


Ile Val Ala Val Val Thr Gly Ile Ala Val Ala Ala Ile Val Ala Ala 
225                 230                 235                 240 


Val Val Ala Leu Ile Tyr Cys Arg Lys Lys Arg Ile Ser Ala Asn Ser 
                245                 250                 255     


Thr Asp Pro Val Lys Ala Ala Gln Phe Glu Pro Pro Gly Arg Gln Met 
            260                 265                 270         


Ile Ala Ile Arg Lys Arg Gln Pro Glu Glu Thr Asn Asn Asp Tyr Glu 
        275                 280                 285             


Thr Ala Asp Gly Gly Tyr Met Thr Leu Asn Pro Arg Ala Pro Thr Asp 
    290                 295                 300                 


Asp Asp Lys Asn Ile Tyr Leu Thr Leu Pro Pro Asn Asp His Val Asn 
305                 310                 315                 320 


Ser Asn Asn 
            


<210>  5
<211>  254
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Trp Gln Leu Leu Leu Pro Thr Ala Leu Leu Leu Leu Val Ser Ala 
1               5                   10                  15      


Gly Met Arg Thr Glu Asp Leu Pro Lys Ala Val Val Phe Leu Glu Pro 
            20                  25                  30          


Gln Trp Tyr Arg Val Leu Glu Lys Asp Ser Val Thr Leu Lys Cys Gln 
        35                  40                  45              


Gly Ala Tyr Ser Pro Glu Asp Asn Ser Thr Gln Trp Phe His Asn Glu 
    50                  55                  60                  


Ser Leu Ile Ser Ser Gln Ala Ser Ser Tyr Phe Ile Asp Ala Ala Thr 
65                  70                  75                  80  


Val Asp Asp Ser Gly Glu Tyr Arg Cys Gln Thr Asn Leu Ser Thr Leu 
                85                  90                  95      


Ser Asp Pro Val Gln Leu Glu Val His Ile Gly Trp Leu Leu Leu Gln 
            100                 105                 110         


Ala Pro Arg Trp Val Phe Lys Glu Glu Asp Pro Ile His Leu Arg Cys 
        115                 120                 125             


His Ser Trp Lys Asn Thr Ala Leu His Lys Val Thr Tyr Leu Gln Asn 
    130                 135                 140                 


Gly Lys Gly Arg Lys Tyr Phe His His Asn Ser Asp Phe Tyr Ile Pro 
145                 150                 155                 160 


Lys Ala Thr Leu Lys Asp Ser Gly Ser Tyr Phe Cys Arg Gly Leu Phe 
                165                 170                 175     


Gly Ser Lys Asn Val Ser Ser Glu Thr Val Asn Ile Thr Ile Thr Gln 
            180                 185                 190         


Gly Leu Ala Val Ser Thr Ile Ser Ser Phe Phe Pro Pro Gly Tyr Gln 
        195                 200                 205             


Val Ser Phe Cys Leu Val Met Val Leu Leu Phe Ala Val Asp Thr Gly 
    210                 215                 220                 


Leu Tyr Phe Ser Val Lys Thr Asn Ile Arg Ser Ser Thr Arg Asp Trp 
225                 230                 235                 240 


Lys Asp His Lys Phe Lys Trp Arg Lys Asp Pro Gln Asp Lys 
                245                 250                 


<210>  6
<211>  233
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Trp Gln Leu Leu Leu Pro Thr Ala Leu Leu Leu Leu Val Ser Ala 
1               5                   10                  15      


Gly Met Arg Thr Glu Asp Leu Pro Lys Ala Val Val Phe Leu Glu Pro 
            20                  25                  30          


Gln Trp Tyr Ser Val Leu Glu Lys Asp Ser Val Thr Leu Lys Cys Gln 
        35                  40                  45              


Gly Ala Tyr Ser Pro Glu Asp Asn Ser Thr Gln Trp Phe His Asn Glu 
    50                  55                  60                  


Ser Leu Ile Ser Ser Gln Ala Ser Ser Tyr Phe Ile Asp Ala Ala Thr 
65                  70                  75                  80  


Val Asn Asp Ser Gly Glu Tyr Arg Cys Gln Thr Asn Leu Ser Thr Leu 
                85                  90                  95      


Ser Asp Pro Val Gln Leu Glu Val His Ile Gly Trp Leu Leu Leu Gln 
            100                 105                 110         


Ala Pro Arg Trp Val Phe Lys Glu Glu Asp Pro Ile His Leu Arg Cys 
        115                 120                 125             


His Ser Trp Lys Asn Thr Ala Leu His Lys Val Thr Tyr Leu Gln Asn 
    130                 135                 140                 


Gly Lys Asp Arg Lys Tyr Phe His His Asn Ser Asp Phe His Ile Pro 
145                 150                 155                 160 


Lys Ala Thr Leu Lys Asp Ser Gly Ser Tyr Phe Cys Arg Gly Leu Val 
                165                 170                 175     


Gly Ser Lys Asn Val Ser Ser Glu Thr Val Asn Ile Thr Ile Thr Gln 
            180                 185                 190         


Gly Leu Ala Val Ser Thr Ile Ser Ser Phe Ser Pro Pro Gly Tyr Gln 
        195                 200                 205             


Val Ser Phe Cys Leu Val Met Val Leu Leu Phe Ala Val Asp Thr Gly 
    210                 215                 220                 


Leu Tyr Phe Ser Val Lys Thr Asn Ile 
225                 230             


