                         SEQUENCE LISTING

<110>  Total S.A.
 
<120>  PROCESS FOR POLYLACTIC ACID PRODUCTION USING MONASCUS

<130>  TOTAL-137-PCT

<150>  EP-A-10290583.3
<151>  2010-10-28

<150>  US61/407747
<151>  2010-10-28

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  999
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized bovine LDH sequence


<220>
<221>  Intron
<222>  (503)..(566)

<400>  1
atggcaaccc tgaaggacca gcttatccaa aacctgctca aggaagaaca cgtcccccaa       60

aacaagatta ctatcgtcgg agtcggtgcc gttggaatgg cctgcgctat ctccattctg      120

atgaaggacc tcgcagatga ggtggcgctc gtcgacgtta tggaggataa gctcaagggt      180

gaaatgatgg accttcagca tggatcgctt ttcttgcgca cacccaagat cgtcagcggc      240

aaggattaca acgttacagc aaattcccgc cttgtgatca ttactgcagg cgcgcgtcag      300

caagagggtg aatcgagact gaacctcgtc caaaggaacg ttaatatctt caagttcatc      360

atccccaaca ttgtcaagta ctccccaaat tgtaagctgc tcgtcgtttc taatccggtt      420

gacatcctca cttatgtggc ctggaagatt tctggcttcc ctaagaaccg agttatcggc      480

tcgggttgca atcttgatag cgctcgattt cggtatctta tgggagagag attgggcgtc      540

catcccctgt cctgtcacgg ctggatcttg ggagaacacg gcgactcctc tgtgccagtc      600

tggtcgggtg tcaacgtggc cggagtcagc cttaagaatt tgcatccgga gttgggcacc      660

gacgccgata aggaacagtg gaaggctgtc cacaagcaag tggtcgactc cgcatacgag      720

gttatcaagc tgaagggata tacctcctgg gcgattggcc tctctgtggc cgatcttgct      780

gaaagcatca tgaagaactt gcgccgtgtc catcctatct ccacgatgat taagggtctg      840

tacggaatta aggaagacgt gtttttgtct gtcccctgca tcctgggcca gaatggtatt      900

tcggatgttg tgaaggtcac cctcacgcac gaggaagagg cttgcttgaa gaagtcggcg      960

gacactctct ggggcatcca aaaggaactc caattttaa                             999


<210>  2
<211>  1015
<212>  DNA
<213>  Monascus ruber


<220>
<221>  Intron
<222>  (503)..(566)

<400>  2
aagtatgcgg ggcaggatgc cacaaaggcc tattctgaag ttcacactcc gagccttatc       60

aaatggaatt tatccccaga caagctcaag ggcactctcg gccaatccac tattgacgat      120

gaatggatga aaccaccgcc aaatgagagc gacaaagttg ttttagagaa cgagaaaccg      180

ccgctgcata tgctgataaa ctcgcacgat ttcgaagtcg tagcttccaa gactgcaagt      240

aagaagacct gggccttcta ttccagcgct gcaacggacc tcatcacccg tgatgccaat      300

aagtcatgct ttgaccggat atggttccga ccccgggtac tgaggaatgt gcgtaccgtc      360

aaaacgcgca cgaagatcct cggggttgac agcagtctcc cacttttcgt gagtccagca      420

gctatggcta agctcattca cccagatggt gagtgtgcca tagcaagggc atgtgcaaat      480

aagggtatca ttcaaggtgt acgttcattg cagattcgaa cacttcccgt tctagttgca      540

accttcttaa catcaatgtc ggataggtgt cgaataactc atcattccca atcgaagagc      600

tccgggaggc ggcaccgtct ggaaatttta ttttccagtt atatgtgaat cgggatcgag      660

agaaatctgc ggaactactc cgcaggtgct cagctaaccc gaacatcaag gccatcttcg      720

tgaccgttga cgcagcctgg cccggtaaac gtgaggcaga cgaacgagtc aaagcggatg      780

agagcctgac agtccccatg tccccatcga caacacgcaa cgacaaaaag gggggcgggc      840

tcgggcgcgt tatggctggg ttcatcgacc cggggctcac ctgggaagat ctggcctggg      900

tgcgacaaca tacccatctc cccgtttgtc tgaagggaat tatgtccgca gacgatgcca      960

ttctagccat gaaattggga ctagatggca tcctgctctg caaccacggc ggccg          1015


<210>  3
<211>  1307
<212>  DNA
<213>  Monascus ruber

<400>  3
tgggtgggya aytgyaatga rctgaatgct ggttackccg ccgatggcta cgctcgtatc       60

aagggtatct ctgccctgat caccactttc ggtgtcggtg agctctctgc cgccaacgcc      120

atcgccggtt cctactcgga rcgggtgccc gttgtccaca ttgtcggtga gcccagcacg      180

gcatcccaga acaaccgcca gcttctgcac cacaccctcg gaaacggtga cttcgatgtc      240

ttcgagaaga tcttcgccaa gatctctact tccgtcgtta agattagaga ccccgccaat      300

gccgccacca tgatcgacca cgttctccgg gaatgtgtta tccagagccg ccccgtctac      360

atcggtctgc cctctgatat ggttaccaag aaggttgagg gagcccgcct gaagaccccc      420

atcgacctgt ccctccccga gaaccccaag gagaaggagg actacgtcgt cagcgtcgtt      480

ctcaagtatc tgcacgctgc caagaaccct gtcatcctcg tcgatgccgg tgtcaaccgc      540

cacaatgccc aggctgaggt gcacgagctg atcaagaagt ctggaatccc caccttcatc      600

acccccatgg gcaagggcgg tgttgacgaa accctcccca actacggcgg tgtctacgcc      660

ggtaccggtt ccaacagggg cgtccgcgag cgtgtcgagg aatctgacct catcctgaac      720

atcggacctc tccagtccga cttcaacacc accggcttct cctaccgcac cagccagctc      780

aacaccatcg agttcgaccg cgacagcgtc caggtccgct actcctacta ccccgacatc      840

cagcttaagg gagttctcca gaacctcatc gccaccatgg gcgaactcaa catcgagcct      900

ggcccgaccc cctccaacgc cctccccgcc aacggcgttg accacgaaac tgcagagatc      960

acccacgaat ggctctggcc catggtcggc aactggctcc gcgaaggtga tgttgtcctc     1020

actgaaaccg gtaccgccaa cttcggtatc tgggaaaccc gcttccccaa gaacgttcag     1080

gccatctccc aggtcctctg gggttccatc ggttactccg tcggtgcctg cttgggtgct     1140

gctctcgccg ctcgggaact tggcgacaac cgtgtcctac tcttcgtcgg tgatggtagc     1200

ttycagatga ccgcccagga gatcagcact atgatccgtc agggattgaa gcctattgtc     1260

ttcgtcatct gcaacaacgg ctacacmatc garmgctaca tccacgg                   1307


<210>  4
<211>  1287
<212>  DNA
<213>  Monascus ruber

<400>  4
ctgaacgccg sctacgccgc cgacggatay gctcgtgtca atggaatcgc tgccttggtt       60

actactttcg gtgtaggaga gctgtcagca gtgaacgcca ttgcgggagc ctactcagag      120

tatgtaccca tcgttcacat tgttggccaa ccaaatacaa ggtcacagag agatggaatg      180

ctgttgcatc atacgttggg caacggcgac tttgatgtct ttaccaagat gagtgcttcc      240

atttcgtgcg ctgttgcaaa gttgaacgac ccccatgaag ctgcaacgct catcgaccac      300

gccattcggg aatgctggat tcgcagcaga ccggtgtaca tcaccctccc tacagacatc      360

gtcacgaaga aagtcgaagg tgaaaggctg aagaccccaa ttgacctgac aatgccagag      420

aatgaagcag aaagggaaga ttacgtggtg gatgttgttt tgaaatacct gcaagccgcg      480

aaaaacccag tcattctagt tgacgcatgc gcaatccgtc acagggtcct ggacgaggta      540

catgaccttg ttaaggcttc tggcttgcca acctttgtga ccccaatggg caaaggagca      600

gtggacgaga ctcatccaaa ttatggtggt gtgtatgctg gagatgggtc taataccggc      660

gtccgtgaag ttgttgaagc ttccgacctt attctgagca ttggcgccat taaatccgat      720

ttcaatactg ccggcttcac ataccgcatc ggccaactta acacgatcga cttccatagt      780

accttcgtgc gagtgaggta ttcggagtac ccgaacacaa acatgaaggg tgttctaagg      840

aagatcatcc agaaaatggg ccccctcagc aagacgccta ttccaaagac tatcaacaag      900

gttccagaac atatcaaagc ttctggtgac acgcggatta ctcatgcttg gttgtggccg      960

acagtcggac agtggctgca gccggaggat gttgtcgtca ctgagactgg aactgcaaac     1020

tttggaatct gggaaaccag gttcccacac ggtgtcacgg ctataagcca agtcctctgg     1080

ggaagcattg ggtacacggt tggagcatgt caaggcgccg cactagctgc aaaggagata     1140

ggcaaccgtc gcactgtact ttttgttggc gatggcagtt tccagcttac cgcgcaggaa     1200

gtgagcacca tgctcagaaa taagctgaac ccgatcattt ttgtgatctg taacgaaggg     1260

tayacratcg agcgctacat ccatggc                                         1287


<210>  5
<211>  1680
<212>  DNA
<213>  Monascus ruber

<400>  5
atgaccactg tcggaagcta cctcgcagaa aggctctccc aaattggcat cgagcgccac       60

ttcgtcgtcc caggcgacta caacctcatc ctcctcgaca aactccaaca acaccccaaa      120

ctcgacgaaa tcaactgcac aaatgaacta aactgctcca tggccgcaga aggctacgcc      180

cgcgcaaaag gcgtagccgc ctgcgtcgtg acgttcagcg tcggcgcatt ctccgcattc      240

aacggcatcg gcagcgcata cggtgagaat ctccctgtca tcctcatctc cggttcccct      300

aataccaacg atcttggctc gtcgcatttg ctgcatcata cgatcggtac gcataatttt      360

gactaccagc ttgagatggc gaaaaatatc acctgctgtg ctgttgcgat tcgtcgtgcc      420

tcggatgcgc cgcggttgat cgacgaggct attcgcaccg cacttcgggc gcggaaacca      480

gcgtatattg agattcctac gaacctctcg ggccagccgt gttccctgcc cggaccggcg      540

tcggggattc tcaagccgga tacgagtgat attgatactc ttgcggaagc gctgaaggca      600

gccaacgact tcctctctac ccggaacaag gtgtccttac tggttggccc taaggttcgc      660

gcagcaggcg ctgaacatgc cgtgatccat cttgctcaag ccctgggatg cgcggtggcc      720

gtgctaccca gtgccaaatc gttcttcccg gagactcatc cgcagttcgt gggtgtatac      780

tggggcgaag tgagcacgaa gggcgcgaat gctatcgtcg actggtccga tacccttatt      840

tgcgtgggga cggtttacac cgactacagc accgttggat ggacggggct acccgaagca      900

gccagtctga ccattgacct ggaccatgtc agtttccctg gatccgatta caaccagatc      960

catatgctgg agttcgtggc aggactggcg aagctggtga agaagaaccc cctgacactc     1020

gtcgaatata accgtctgca accagaccct ctcgttcaca cgccatctcc gccggatcaa     1080

cgactgagcc ggcgagaaat gcagtaccag atcagccagt tcctgacgcg caacacgacg     1140

gtcgttgtgg acacgggcga ctcgtggttc aacgggatgc agatggacct tccggacgga     1200

gtgagatttg aggttgagat gcaatgggga cacatcggat ggtccgttcc agcagcactg     1260

ggtctggccg ccgcaaaccc cgagcgacag atagtcgtca tggtaggcga tgggtcgttc     1320

cagatgacgg gccacgaggt gtcaaatatg actcgattag ggctaccgat tatcatcttc     1380

ctgatcaaca atgacgggta cacaatcgaa gtcgagatcc acgatggcat ctacaacaac     1440

atcaagaact gggattacgg cgcgttcgtc gagtcgttca atgccaagga gggacatggg     1500

aaggggtatc gtgttaccac ggcgggggaa atgcacaggg ccattgaggc ggcgaagaag     1560

aataaacagg ggccaacact aatcgagtgt gatattgatc gcgatgattg cagtaaggag     1620

ttgatcagtt gggggcatta tgtggctgct gcgaatggca agcctcctgt tgccaggtga     1680


<210>  6
<211>  1655
<212>  DNA
<213>  Monascus ruber

<400>  6
atgtctcaac ctaagcttac cggcgctgat atcgccaaac acaattccaa ggactcgtgc       60

tgggtcattg tccacgggaa agcatacgat gtcacggact tcctgccagg tattgattga      120

cccccctgtc cgggaattcc gagatgctgc ctgcgttcat tgaatactga tttgcatgaa      180

tttgatcaat tatagaacat cccggtggcc agaagattat cctgaagtat gccggcagag      240

atgccacgga agaattcgag cccatccacc ccccggatac cctggacaag tacctcgaca      300

agtcaaagca cttgggcgag gtcgacatgg ccactgtcgc acacgacgag aaggctgtcg      360

atcccgagga gacggctcgc caggaaagaa tcaagctcat gccatcgttg caatcctgct      420

acaatctgat ggactttgaa tccgtggcgc agcaggtcat gaagaagact gcgtgggcat      480

actactcaag tggtgctgat gatgaaatcg tatgaccata tctggatttc tcgttccctt      540

tgcagcacat actgacttgc gtctgttcac agaccctgcg agaaaaccac actgccttcc      600

ataagatctg gttccggccg cgagtcctag tcgacgtgga acatgtcgac tactctacga      660

ccatgctggg aaccaaggtc tccgctccct tctatgtgac ggccacagcc ctgggcaaac      720

tgggacaccc cgagggtgag gtcgttctca cccgttcctc ctaccgtcac aacgtcatcc      780

agatgattcc cacgctcgcc tcgtgctcct ttgacgagat tattgacgcc cgccaaggcg      840

atcaggtcca gtggctgcag ctctacgtca acaagaaccg cgatatcacc aagcgcattg      900

tgcaacatgc cgaagcccgc ggctgcaagg gcctcttcat caccgtcgac gccccgcaat      960

taggtcgtcg agagaaagac atgcgctcca agttctccga cgagggctcc aacgtccaga     1020

aagaagaggg tgaggagaac gtcgaccgct ctcagggtgc cgcccgtgct atctcctcgt     1080

tcatcgaccc cgccctctcc tggaaggata tcccctggtt ccaatccatc acgaagatgc     1140

ccatcgtcct gaagggtgtg cagtgcgtcg aagacgtttt ccgtgctatc gaagccggag     1200

tccagggtgt tgtgctgtcc aatcacggtg gccgtcagct cgagttcgca ccatcggctg     1260

tcgagcttct ggccgaggtt atgcctgcgc tgcgccagcg cggcttggag aacagcatcg     1320

aggtgtacat cgacggtggc atccgcagag gcactgatat cgtcaaggcg ctctgccttg     1380

gcgcccaggg cgtggggatt ggtcgtcctt tcctgtacgc catgtcggcg tacggccagg     1440

ccggtgtcga caaggccatg cagcttctca aggatgagat ggagatgaac atgagactca     1500

tcggagccac taaggtctcc gagctgagcc ccagcctcgt cgatacccgc ggtcttcttg     1560

gtggtcacca cgctcctgtt ccgtccgaca cgctgggcat gaaggcgtac gatgcgctcc     1620

aggctccgcg gttcaacgaa aagtcgaagt tgtag                                1655


