                         SEQUENCE LISTING

<110>  AB Enzymes Oy
 
<120>  Variants of fungal serine protease

<130>  B0099PC

<160>  50    

<170>  PatentIn version 3.5

<210>  1
<211>  870
<212>  DNA
<213>  Fusarium equiseti


<220>
<221>  MISC_FEATURE
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 protease

<400>  1
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gctggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tgtcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

gccatggctt tcaacggcgc tactgcttaa                                       870


<210>  2
<211>  289
<212>  PRT
<213>  Fusarium equiseti


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of the mature form of Fe_RF6318 protease

<400>  2

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Ala Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Val 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Ala Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  3
<211>  870
<212>  DNA
<213>  Fusarium acuminatum


<220>
<221>  MISC_FEATURE
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fusarium acuminatum RF7182 protease

<400>  3
gctctgacca cccagtctgg tgctccctgg ggtctcggtg ccatctccca caagtcgtct       60

ggctccacca gctacatcta cgacaccact gccggtagcg gctcttacgg atatgtcgta      120

gatagtggta tcaacatcgc ccataccgac tttggtggcc gtgctactct cggctacaac      180

gctgctggtg gtgctcacac cgataccctc ggccacggaa cccacgttgc tggtaccatc      240

ggtggcacca agtatggtgt ctccaagaag gccaacctca tctctgtcaa ggtcttcgcc      300

ggtaaccagg ctgctacatc tgttatcctt gatggcttta actgggccgt caacgacatc      360

acctccaagg gccgtgctgg caagtccgtt atcaacatgt ctctcggcgg accttcttct      420

gctacttgga ccactgccat caacgctgga tacaacgctg gtgtcctctc cgttgtcgct      480

gccggtaacg gtgatgtcaa tggcaaccct ctccccgtct ctagccagtc tcctgccaac      540

gcccccaacg ccctgaccgt cgctgccatt gactccaact ggcgcactgc ctctttcacc      600

aactacggtg ccggtgttga tatcttcggc cccggtgtca acattctgtc cgcctggatc      660

ggctccagca ccgctaccaa caccatcagc ggaacttcca tggcctcccc ccaccttgct      720

ggtcttgctc tctacctcca ggtccttgag ggtcttagca ctcctgctgc tgtcaccaac      780

cgcatcaagg ctcttggtac ctctggcaag gtcactggta gcctcagcgg cagccccaac      840

ctcgttgcct acaacggtaa cggtgcttag                                       870


<210>  4
<211>  289
<212>  PRT
<213>  Fusarium acuminatum


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of the mature form of Fusarium acuminatum
       RF7182 protease

<400>  4

Ala Leu Thr Thr Gln Ser Gly Ala Pro Trp Gly Leu Gly Ala Ile Ser 
1               5                   10                  15      


His Lys Ser Ser Gly Ser Thr Ser Tyr Ile Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ser Gly Ser Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Ile Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Thr Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Gly Gly Thr Lys Tyr Gly Val Ser Lys Lys Ala Asn Leu Ile Ser Val 
                85                  90                  95      


Lys Val Phe Ala Gly Asn Gln Ala Ala Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Gly Arg Ala Gly Lys 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Ala Thr Trp Thr 
    130                 135                 140                 


Thr Ala Ile Asn Ala Gly Tyr Asn Ala Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Val Asn Gly Asn Pro Leu Pro Val Ser Ser Gln 
                165                 170                 175     


Ser Pro Ala Asn Ala Pro Asn Ala Leu Thr Val Ala Ala Ile Asp Ser 
            180                 185                 190         


Asn Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Ala Gly Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Leu Ser Ala Trp Ile Gly Ser Ser Thr 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His Leu Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Val Leu Glu Gly Leu Ser Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ala Leu Gly Thr Ser Gly Lys Val Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Val Ala Tyr Asn Gly Asn Gly 
        275                 280                 285             


Ala 
    


<210>  5
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitution V208I

<400>  5
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gctggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

gccatggctt tcaacggcgc tactgcttaa                                       870


<210>  6
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitution V208I

<400>  6

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Ala Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Ala Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  7
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I and A111D

<400>  7
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

gccatggctt tcaacggcgc tactgcttaa                                       870


<210>  8
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I and A111D

<400>  8

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Ala Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  9
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I and A281L

<400>  9
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gctggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  10
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I and A281L

<400>  10

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Ala Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  11
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D and A281L

<400>  11
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  12
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D and A281L

<400>  12

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  13
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L and A33E

<400>  13
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgagg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  14
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L and
       A33E

<400>  14

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Glu Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  15
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L and A47E

<400>  15
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccga gcacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  16
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L and
       A47E

<400>  16

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Glu His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  17
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L and G63P

<400>  17
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctcccg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  18
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L and
       G63P

<400>  18

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Pro Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  19
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L and I185M

<400>  19
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatgaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  20
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L and
       I185M

<400>  20

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Met Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  21
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L and T268R

<400>  21
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac tcgaggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  22
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L and
       T268R

<400>  22

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Arg Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  23
<211>  873
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L, Q103A, insertion G104

<400>  23
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaacgccg gcgcttctac ctctgttatc cttgacggtt tcaactgggc tgtcaacgac      360

atcacctcca agaaccgtgc tagccgctct gtcatcaaca tgtctctcgg tggtccctct      420

tctcagacct gggctactgc catcaacgct gcctacagcc aaggtgtcct ctccgttgtt      480

gctgccggta acggtgattc caacggtcgt cctctccccg cctctggcca gtctcctgcc      540

aacgttccca acgctatcac cgttgctgcc gccgactcca gctggcgaac tgcctctttc      600

accaactacg gtcctgaggt cgatatcttc ggtcctggtg tcaacatcca gtccacctgg      660

tacacctcca acagcgctac caacaccatc agcggtacct ccatggcttg ccctcacgtt      720

gctggtcttg ctctctacct ccaggctctc gagaacctca atacccctgc tgccgtcacc      780

aaccgcatca agtctcttgc cactaccggc cgcatcactg gcagcctcag cggcagcccc      840

aacctcatgg ctttcaacgg cgctactgct taa                                   873


<210>  24
<211>  290
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L, 
       Q103A, insertion G104

<400>  24

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Ala Gly Ala Ser Thr Ser Val Ile Leu Asp 
            100                 105                 110         


Gly Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser 
        115                 120                 125             


Arg Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp 
    130                 135                 140                 


Ala Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val 
145                 150                 155                 160 


Ala Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly 
                165                 170                 175     


Gln Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp 
            180                 185                 190         


Ser Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp 
        195                 200                 205             


Ile Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn 
    210                 215                 220                 


Ser Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val 
225                 230                 235                 240 


Ala Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro 
                245                 250                 255     


Ala Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile 
            260                 265                 270         


Thr Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala 
        275                 280                 285             


Thr Ala 
    290 


<210>  25
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L, T3C and T29C

<400>  25
gctctgtgca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgactgcact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  26
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L, 
       T3C and T29C

<400>  26

Ala Leu Cys Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Cys Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  27
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L, S6R and T24D

<400>  27
gctctgacca ctcagcgcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagcg actacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  28
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L, 
       S6R and T24D

<400>  28

Ala Leu Thr Thr Gln Arg Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Asp Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  29
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L and V100D

<400>  29
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgac      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  30
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L and
       V100D

<400>  30

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Asp Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  31
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L and V100K

<400>  31
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcaag      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  32
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L and
       V100K

<400>  32

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Lys Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  33
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L and T106N

<400>  33
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctaactc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  34
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L and
       T106N

<400>  34

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Asn Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  35
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L, T3C, T29C, S6R and T24D

<400>  35
gctctgtgca ctcagcgcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagcg actacaccta cgactgcact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  36
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L, 
       T3C, T29C, S6R and T24D

<400>  36

Ala Leu Cys Thr Gln Arg Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Asp Tyr Thr Tyr Asp Cys Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  37
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L, I185M and G210A

<400>  37
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatgaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcgcc cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  38
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L, 
       I185M and G210A

<400>  38

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Met Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Ala Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  39
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L and V100Q

<400>  39
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttccag      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  40
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L and
       V100Q

<400>  40

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Gln Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  41
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L and K123R

<400>  41
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctcccgca accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  42
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L and
       K123R

<400>  42

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Arg Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  43
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L and S157T

<400>  43
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctcac cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  44
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L and
       S157T

<400>  44

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Thr Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  45
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L and G175S

<400>  45
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct cttcccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  46
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L and
       G175S

<400>  46

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Ser Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  47
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L and Q176T

<400>  47
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggcacctc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggcttgccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  48
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L and
       Q176T

<400>  48

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Thr 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


<210>  49
<211>  870
<212>  DNA
<213>  Artificial sequence

<220>
<223>  The nucleotide sequence encoding the amino acid sequence of the 
       mature form of Fe_RF6318 serine protease variant comprising 
       substitutions V208I, A111D, A281L and C236T

<400>  49
gctctgacca ctcagagcaa cgctccttgg ggtcttgctg ccatctcccg ccgaaccccc       60

ggtggcagca cctacaccta cgacaccact gccggtgccg gtacttacgg ttacgtcgtt      120

gactctggta tcaacaccgc ccacactgac tttggcggcc gtgcttctct cggttacaac      180

gctgctggtg gcgcccacac tgataccctt ggccacggta cccacgttgc tggtaccatt      240

gcctccaaca cctacggtgt tgccaagcgt gccaacgtca tctctgtcaa ggttttcgtc      300

ggtaaccaag cttctacctc tgttatcctt gacggtttca actgggctgt caacgacatc      360

acctccaaga accgtgctag ccgctctgtc atcaacatgt ctctcggtgg tccctcttct      420

cagacctggg ctactgccat caacgctgcc tacagccaag gtgtcctctc cgttgttgct      480

gccggtaacg gtgattccaa cggtcgtcct ctccccgcct ctggccagtc tcctgccaac      540

gttcccaacg ctatcaccgt tgctgccgcc gactccagct ggcgaactgc ctctttcacc      600

aactacggtc ctgaggtcga tatcttcggt cctggtgtca acatccagtc cacctggtac      660

acctccaaca gcgctaccaa caccatcagc ggtacctcca tggctacccc tcacgttgct      720

ggtcttgctc tctacctcca ggctctcgag aacctcaata cccctgctgc cgtcaccaac      780

cgcatcaagt ctcttgccac taccggccgc atcactggca gcctcagcgg cagccccaac      840

ctcatggctt tcaacggcgc tactgcttaa                                       870


<210>  50
<211>  289
<212>  PRT
<213>  Artificial sequence

<220>
<223>  The amino acid sequence of the mature form of Fe_RF6318 serine 
       protease variant comprising substitutions V208I, A111D, A281L and
       C236T

<400>  50

Ala Leu Thr Thr Gln Ser Asn Ala Pro Trp Gly Leu Ala Ala Ile Ser 
1               5                   10                  15      


Arg Arg Thr Pro Gly Gly Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly 
            20                  25                  30          


Ala Gly Thr Tyr Gly Tyr Val Val Asp Ser Gly Ile Asn Thr Ala His 
        35                  40                  45              


Thr Asp Phe Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly 
    50                  55                  60                  


Ala His Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile 
65                  70                  75                  80  


Ala Ser Asn Thr Tyr Gly Val Ala Lys Arg Ala Asn Val Ile Ser Val 
                85                  90                  95      


Lys Val Phe Val Gly Asn Gln Ala Ser Thr Ser Val Ile Leu Asp Gly 
            100                 105                 110         


Phe Asn Trp Ala Val Asn Asp Ile Thr Ser Lys Asn Arg Ala Ser Arg 
        115                 120                 125             


Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser Gln Thr Trp Ala 
    130                 135                 140                 


Thr Ala Ile Asn Ala Ala Tyr Ser Gln Gly Val Leu Ser Val Val Ala 
145                 150                 155                 160 


Ala Gly Asn Gly Asp Ser Asn Gly Arg Pro Leu Pro Ala Ser Gly Gln 
                165                 170                 175     


Ser Pro Ala Asn Val Pro Asn Ala Ile Thr Val Ala Ala Ala Asp Ser 
            180                 185                 190         


Ser Trp Arg Thr Ala Ser Phe Thr Asn Tyr Gly Pro Glu Val Asp Ile 
        195                 200                 205             


Phe Gly Pro Gly Val Asn Ile Gln Ser Thr Trp Tyr Thr Ser Asn Ser 
    210                 215                 220                 


Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr Pro His Val Ala 
225                 230                 235                 240 


Gly Leu Ala Leu Tyr Leu Gln Ala Leu Glu Asn Leu Asn Thr Pro Ala 
                245                 250                 255     


Ala Val Thr Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr 
            260                 265                 270         


Gly Ser Leu Ser Gly Ser Pro Asn Leu Met Ala Phe Asn Gly Ala Thr 
        275                 280                 285             


Ala 
    


