                         SEQUENCE LISTING

<110>  Sued-Chemie
 
<120>  Temperaturstabile beta-Pyranosidase

<130>  HE 150704

<160>  4     

<170>  PatentIn version 3.4

<210>  1
<211>  775
<212>  PRT
<213>  Fervidobacterium gondwanense

<400>  1

Met Glu Ile Tyr Lys Asp Ser Ser Lys Pro Ile Glu Leu Arg Val Glu 
1               5                   10                  15      


Asp Leu Leu Ser Arg Met Thr Leu Glu Glu Lys Val Ser Gln Leu Gly 
            20                  25                  30          


Ser Val Trp Ser Tyr Gln Leu Leu Asp Glu Asn Gly Asn Phe Asp Glu 
        35                  40                  45              


Gly Lys Ala Phe Glu Leu Leu Lys Asp Gly Ile Gly Gln Ile Ser Arg 
    50                  55                  60                  


Pro Gly Gly Ala Thr Asn Phe Gln Pro Glu Glu Val Ala Gln Phe Asp 
65                  70                  75                  80  


Asn Lys Val Gln Lys Phe Leu Ile Glu Asn Thr Arg Leu Gly Ile Pro 
                85                  90                  95      


Ala Leu Met His Glu Glu Cys Leu Thr Gly Tyr Met Gly Leu Asn Gly 
            100                 105                 110         


Ser Asn Phe Pro Val Pro Ile Ala Met Ala Ser Thr Trp Glu Pro Glu 
        115                 120                 125             


Leu Val Lys Glu Val Thr Lys Val Ile Arg Gln Glu Met Arg Asn Met 
    130                 135                 140                 


Gly Ile His Gln Gly Leu Ala Pro Val Leu Asp Val Ala Arg Asp Pro 
145                 150                 155                 160 


Arg Trp Gly Arg Val Glu Glu Thr Phe Gly Glu Ser Pro Tyr Leu Val 
                165                 170                 175     


Ala Ser Met Gly Cys Ala Tyr Val Glu Gly Leu Gln Gly Glu Asp Leu 
            180                 185                 190         


Lys Asp Gly Val Ile Ala Thr Thr Lys His Phe Val Gly Tyr Ser Ala 
        195                 200                 205             


Ser Glu Gly Gly Arg Asn Trp Ala Pro Thr Asn Ile Pro Pro Arg Glu 
    210                 215                 220                 


Leu Arg Glu Ile Phe Met Phe Pro Phe Glu Ala Ala Val Lys Val Ala 
225                 230                 235                 240 


Lys Val Gly Ser Val Met Asn Ser Tyr Ser Glu Ile Asp Gly Val Pro 
                245                 250                 255     


Leu Ala Ala Ser Arg Glu Leu Leu Thr Asp Val Leu Arg Lys Glu Trp 
            260                 265                 270         


Gly Phe Asp Gly Leu Val Val Ser Asp Tyr Phe Ser Val Lys Leu Ile 
        275                 280                 285             


His Glu His His Lys Leu Ala Arg Asp Lys Ala Glu Ala Ala Lys Tyr 
    290                 295                 300                 


Ala Leu Glu Ala Gly Ile Asp Val Glu Leu Pro Asn Thr Asp Cys Tyr 
305                 310                 315                 320 


Ala His Val Leu Asp Leu Val Lys Ser Gly Val Ile Pro Glu Lys Leu 
                325                 330                 335     


Leu Asp Gln Thr Val Arg Arg Ile Leu Lys Met Lys Phe Lys Leu Gly 
            340                 345                 350         


Leu Phe Asp Lys Pro Tyr Val Glu Pro Ser Lys Ala Lys Val Val Lys 
        355                 360                 365             


Asn Thr Glu Leu Ala Leu Glu Val Ala Arg Lys Ser Ile Val Leu Leu 
    370                 375                 380                 


Lys Asn Asp Gly Ile Leu Pro Leu Lys Lys Asp Met Lys Val Ala Leu 
385                 390                 395                 400 


Ile Gly Pro Asn Ala Ala Asp Val Arg Asn Met Leu Gly Asp Tyr Met 
                405                 410                 415     


Tyr Leu Ala His Ile Lys Ile Met Leu Glu Asn Val Asn Leu Ala Phe 
            420                 425                 430         


Asp Ala Pro Lys Phe Asn Leu Ser Ser Val Lys Lys Ser Val Glu Glu 
        435                 440                 445             


Ser Met Asn Lys Ile Lys Ser Ile Glu Met Leu Leu Lys Glu Glu Ser 
    450                 455                 460                 


Val Gln Phe Thr Tyr Ala Lys Gly Cys Asp Val Leu Gly Asp Ser Lys 
465                 470                 475                 480 


Glu Gly Phe Asn Glu Ala Leu Lys Ala Val Glu Asn Ser Asp Val Ala 
                485                 490                 495     


Ile Val Val Val Gly Asp Arg Ser Gly Leu Thr Met Asp Cys Thr Thr 
            500                 505                 510         


Gly Glu Ser Arg Asp Ser Ala Asn Leu Lys Leu Pro Gly Val Gln Glu 
        515                 520                 525             


Glu Leu Ile Ile Glu Val Ser Lys Val Gly Lys Pro Val Val Leu Ala 
    530                 535                 540                 


Leu Leu Asn Gly Arg Pro Tyr Ser Leu Thr Arg Val Val Asp Lys Val 
545                 550                 555                 560 


Ser Ala Ile Val Glu Ala Trp Leu Pro Gly Glu Ile Gly Ala Lys Ala 
                565                 570                 575     


Ile Val Asp Val Leu Tyr Gly Lys Val Asn Pro Ser Gly Lys Leu Pro 
            580                 585                 590         


Met Thr Phe Pro Arg Ser Ala Gly Gln Ile Pro Leu Phe His Tyr Phe 
        595                 600                 605             


Lys Pro Ser Gly Gly Arg Ser Ser Trp His Gly Asp Tyr Val Asp Glu 
    610                 615                 620                 


Ser Val Lys Pro Leu Phe Pro Phe Gly His Gly Leu Ser Tyr Thr Asn 
625                 630                 635                 640 


Phe Asp Tyr Ser Gly Leu Glu Ile Ser Pro Ser Lys Val Pro Met Ala 
                645                 650                 655     


Gly Ser Val Glu Ile Ser Leu Tyr Val Glu Asn Thr Gly Glu Val Glu 
            660                 665                 670         


Gly Glu Glu Val Val Gln Leu Tyr Ile Gly Arg Glu Cys Ala Ser Val 
        675                 680                 685             


Thr Arg Pro Val Lys Glu Leu Lys Gly Phe Ala Lys Val Asn Leu Lys 
    690                 695                 700                 


Pro Gly Glu Lys Arg Lys Val Leu Phe Asn Leu His Thr Asp Val Leu 
705                 710                 715                 720 


Ala Phe Tyr Gly Arg Asp Met Lys Leu Cys Val Glu Pro Gly Val Tyr 
                725                 730                 735     


Asn Val Met Ile Gly Ser Ser Ser Asp Asp Ile Arg Leu Lys Gly Ser 
            740                 745                 750         


Phe Glu Val Asp Gly Met Arg Arg Glu Val Phe Glu Asp Arg Val Phe 
        755                 760                 765             


Phe Thr Lys Val Tyr Thr Phe 
    770                 775 


<210>  2
<211>  625
<212>  PRT
<213>  artificial

<220>
<223>  segment consisting of amino acid residues 13 to 638 of SEQ ID NO 
       1

<400>  2

Arg Val Glu Asp Leu Leu Ser Arg Met Thr Leu Glu Glu Lys Val Ser 
1               5                   10                  15      


Gln Leu Gly Ser Val Trp Ser Tyr Gln Leu Leu Asp Glu Asn Gly Asn 
            20                  25                  30          


Phe Asp Glu Gly Lys Ala Phe Glu Leu Leu Lys Asp Gly Ile Gly Gln 
        35                  40                  45              


Ile Ser Arg Pro Gly Gly Ala Thr Asn Phe Gln Pro Glu Glu Val Ala 
    50                  55                  60                  


Gln Phe Asp Asn Lys Val Gln Lys Phe Leu Ile Glu Asn Thr Arg Leu 
65                  70                  75                  80  


Gly Ile Pro Ala Leu Met His Glu Glu Cys Leu Thr Gly Tyr Met Gly 
                85                  90                  95      


Leu Asn Gly Ser Asn Phe Pro Val Pro Ile Ala Met Ala Ser Thr Trp 
            100                 105                 110         


Glu Pro Glu Leu Val Lys Glu Val Thr Lys Val Ile Arg Gln Glu Met 
        115                 120                 125             


Arg Asn Met Gly Ile His Gln Gly Leu Ala Pro Val Leu Asp Val Ala 
    130                 135                 140                 


Arg Asp Pro Arg Trp Gly Arg Val Glu Glu Thr Phe Gly Glu Ser Pro 
145                 150                 155                 160 


Tyr Leu Val Ala Ser Met Gly Cys Ala Tyr Val Glu Gly Leu Gln Gly 
                165                 170                 175     


Glu Asp Leu Lys Asp Gly Val Ile Ala Thr Thr Lys His Phe Val Gly 
            180                 185                 190         


Tyr Ser Ala Ser Glu Gly Gly Arg Asn Trp Ala Pro Thr Asn Ile Pro 
        195                 200                 205             


Pro Arg Glu Leu Arg Glu Ile Phe Met Phe Pro Phe Glu Ala Ala Val 
    210                 215                 220                 


Lys Val Ala Lys Val Gly Ser Val Met Asn Ser Tyr Ser Glu Ile Asp 
225                 230                 235                 240 


Gly Val Pro Leu Ala Ala Ser Arg Glu Leu Leu Thr Asp Val Leu Arg 
                245                 250                 255     


Lys Glu Trp Gly Phe Asp Gly Leu Val Val Ser Asp Tyr Phe Ser Val 
            260                 265                 270         


Lys Leu Ile His Glu His His Lys Leu Ala Arg Asp Lys Ala Glu Ala 
        275                 280                 285             


Ala Lys Tyr Ala Leu Glu Ala Gly Ile Asp Val Glu Leu Pro Asn Thr 
    290                 295                 300                 


Asp Cys Tyr Ala His Val Leu Asp Leu Val Lys Ser Gly Val Ile Pro 
305                 310                 315                 320 


Glu Lys Leu Leu Asp Gln Thr Val Arg Arg Ile Leu Lys Met Lys Phe 
                325                 330                 335     


Lys Leu Gly Leu Phe Asp Lys Pro Tyr Val Glu Pro Ser Lys Ala Lys 
            340                 345                 350         


Val Val Lys Asn Thr Glu Leu Ala Leu Glu Val Ala Arg Lys Ser Ile 
        355                 360                 365             


Val Leu Leu Lys Asn Asp Gly Ile Leu Pro Leu Lys Lys Asp Met Lys 
    370                 375                 380                 


Val Ala Leu Ile Gly Pro Asn Ala Ala Asp Val Arg Asn Met Leu Gly 
385                 390                 395                 400 


Asp Tyr Met Tyr Leu Ala His Ile Lys Ile Met Leu Glu Asn Val Asn 
                405                 410                 415     


Leu Ala Phe Asp Ala Pro Lys Phe Asn Leu Ser Ser Val Lys Lys Ser 
            420                 425                 430         


Val Glu Glu Ser Met Asn Lys Ile Lys Ser Ile Glu Met Leu Leu Lys 
        435                 440                 445             


Glu Glu Ser Val Gln Phe Thr Tyr Ala Lys Gly Cys Asp Val Leu Gly 
    450                 455                 460                 


Asp Ser Lys Glu Gly Phe Asn Glu Ala Leu Lys Ala Val Glu Asn Ser 
465                 470                 475                 480 


Asp Val Ala Ile Val Val Val Gly Asp Arg Ser Gly Leu Thr Met Asp 
                485                 490                 495     


Cys Thr Thr Gly Glu Ser Arg Asp Ser Ala Asn Leu Lys Leu Pro Gly 
            500                 505                 510         


Val Gln Glu Glu Leu Ile Ile Glu Val Ser Lys Val Gly Lys Pro Val 
        515                 520                 525             


Val Leu Ala Leu Leu Asn Gly Arg Pro Tyr Ser Leu Thr Arg Val Val 
    530                 535                 540                 


Asp Lys Val Ser Ala Ile Val Glu Ala Trp Leu Pro Gly Glu Ile Gly 
545                 550                 555                 560 


Ala Lys Ala Ile Val Asp Val Leu Tyr Gly Lys Val Asn Pro Ser Gly 
                565                 570                 575     


Lys Leu Pro Met Thr Phe Pro Arg Ser Ala Gly Gln Ile Pro Leu Phe 
            580                 585                 590         


His Tyr Phe Lys Pro Ser Gly Gly Arg Ser Ser Trp His Gly Asp Tyr 
        595                 600                 605             


Val Asp Glu Ser Val Lys Pro Leu Phe Pro Phe Gly His Gly Leu Ser 
    610                 615                 620                 


Tyr 
625 


<210>  3
<211>  2328
<212>  DNA
<213>  Fervidobacterium gondwanense

<400>  3
atggagatat ataaggattc ttctaagcct attgaattga gagttgagga cctgctttcg     60

agaatgacgc tggaagaaaa ggttagccag ctcggttctg tttggagtta tcaattactc    120

gacgagaacg ggaatttcga tgaagggaaa gcatttgagc tgttgaagga cggcataggt    180

caaatctcaa gaccgggtgg agcaacaaac tttcaacccg aagaagttgc tcaattcgac    240

aataaagtac agaaattctt gatagaaaat acgagacttg gaataccagc attaatgcac    300

gaagaatgcc taactggata tatgggactc aacggttcga attttcctgt gcctatcgcg    360

atggcgagta cgtgggagcc tgaactggta aaggaagtta cgaaagtgat aaggcaagag    420

atgaggaata tgggaattca ccaagggctc gctcctgtac ttgacgttgc aagggaccca    480

agatggggaa gggttgagga aacattcgga gagtcgcctt atcttgtcgc aagtatgggg    540

tgtgcttatg tcgaaggtct gcagggagaa gacttgaaag acggagtcat tgccactaca    600

aagcactttg tcggttacag cgcgtctgaa ggagggcgga actgggctcc aactaacatt    660

ccgccacgcg agttgagaga gatcttcatg ttcccattcg aagctgccgt gaaagtagcg    720

aaggttggtt ctgttatgaa ttcgtacagc gagatagacg gagtgcctct tgccgcctcc    780

agagagcttt taacagatgt gctgaggaaa gaatggggat ttgatggact cgttgtctct    840

gattatttct cggtgaagtt gatccacgaa catcacaaat tagcaaggga taaagccgaa    900

gcagcaaaat acgcccttga agcgggaata gatgtggaat taccaaatac cgattgctat    960

gcacatgtct tagacctggt gaaaagcggg gttattccgg aaaagttgtt agatcaaact   1020

gttagaagga ttttgaagat gaaatttaaa ttgggcttgt tcgataagcc gtacgtagaa   1080

ccttcaaaag cgaaggttgt aaaaaacaca gagcttgctc ttgaagttgc aagaaagtca   1140

atagtgctcc ttaagaacga cggtatcttg ccattgaaaa aagatatgaa agtagcactc   1200

ataggaccaa atgcggcgga tgtcaggaac atgctcggtg attacatgta cttagcacat   1260

ataaaaataa tgctcgagaa cgttaacttg gcttttgatg cgcctaagtt caatctttca   1320

agcgtgaaga aatcggttga ggagagcatg aacaagatca agagtataga gatgttgctc   1380

aaagaagaaa gtgttcaatt cacatacgcg aaaggttgcg atgtcttggg agattcgaaa   1440

gaagggttca acgaggcgct caaagcagtc gagaatagcg atgtggcgat agttgtagtt   1500

ggtgataggt caggtttgac catggattgc acaacgggtg agtcgaggga cagcgcgaat   1560

ttgaagttgc ccggagttca ggaagaactt atcattgagg tttcaaaggt tggcaagcct   1620

gtggtgctcg ctttgctgaa tggcagacct tattctttaa ccagggttgt agacaaggtt   1680

tctgctattg ttgaggcgtg gttgccaggt gagattggtg caaaggcaat agtcgatgtg   1740

ctttacggca aagtcaatcc atccggtaaa cttccaatga ctttcccgag aagtgctggt   1800

cagattccgc tcttccacta cttcaaaccg tctggcggaa ggtccagttg gcatggtgac   1860

tacgttgacg agagtgtcaa accactattc ccgttcggtc acggactttc gtatactaat   1920

ttcgattaca gcgggttaga aatctctcca tcaaaagtgc caatggcagg aagcgtcgaa   1980

atttcacttt atgtggagaa cactggtgaa gttgaaggcg aagaggttgt gcagctttac   2040

atcggaagag aatgtgcctc agtaactcgg ccagttaagg aactgaaagg ttttgcgaag   2100

gtgaatttga aacctggcga aaagaggaaa gttttgttca atctccacac ggacgtgctc   2160

gcgttctacg gacgcgatat gaagctctgc gttgagcctg gggtttacaa cgtcatgatt   2220

ggaagttctt cggatgatat aaggcttaaa ggaagttttg aggtagatgg aatgagaaga   2280

gaggtatttg aagatagagt attctttaca aaagtttaca ctttctaa                2328


<210>  4
<211>  630
<212>  PRT
<213>  Hordeum vulgare

<300>
<301>  Varghese et al.
<303>  Structure
<304>  7
<305>  2
<306>  179-190
<307>  1999      

<400>  4

Met Ala Leu Leu Thr Ala Pro Ala Val Phe Ala Ala Leu Leu Leu Phe 
1               5                   10                  15      


Trp Ala Val Leu Gly Gly Thr Asp Ala Asp Tyr Val Leu Tyr Lys Asp 
            20                  25                  30          


Ala Thr Lys Pro Val Glu Asp Arg Val Ala Asp Leu Leu Gly Arg Met 
        35                  40                  45              


Thr Leu Ala Glu Lys Ile Gly Gln Met Thr Gln Ile Glu Arg Leu Val 
    50                  55                  60                  


Ala Thr Pro Asp Val Leu Arg Asp Asn Phe Ile Gly Ser Leu Leu Ser 
65                  70                  75                  80  


Gly Gly Gly Ser Val Pro Arg Lys Gly Ala Thr Ala Lys Glu Trp Gln 
                85                  90                  95      


Asp Met Val Asp Gly Phe Gln Lys Ala Cys Met Ser Thr Arg Leu Gly 
            100                 105                 110         


Ile Pro Met Ile Tyr Gly Ile Asp Ala Val His Gly Gln Asn Asn Val 
        115                 120                 125             


Tyr Gly Ala Thr Ile Phe Pro His Asn Val Gly Leu Gly Ala Thr Arg 
    130                 135                 140                 


Asp Pro Tyr Leu Val Lys Arg Ile Gly Glu Ala Thr Ala Leu Glu Val 
145                 150                 155                 160 


Arg Ala Thr Gly Ile Gln Tyr Ala Phe Ala Pro Cys Ile Ala Val Cys 
                165                 170                 175     


Arg Asp Pro Arg Trp Gly Arg Cys Tyr Glu Ser Tyr Ser Glu Asp Arg 
            180                 185                 190         


Arg Ile Val Gln Ser Met Thr Glu Leu Ile Pro Gly Leu Gln Gly Asp 
        195                 200                 205             


Val Pro Lys Asp Phe Thr Ser Gly Met Pro Phe Val Ala Gly Lys Asn 
    210                 215                 220                 


Lys Val Ala Ala Cys Ala Lys His Phe Val Gly Asp Gly Gly Thr Val 
225                 230                 235                 240 


Asp Gly Ile Asn Glu Asn Asn Thr Ile Ile Asn Arg Glu Gly Leu Met 
                245                 250                 255     


Asn Ile His Met Pro Ala Tyr Lys Asn Ala Met Asp Lys Gly Val Ser 
            260                 265                 270         


Thr Val Met Ile Ser Tyr Ser Ser Trp Asn Gly Val Lys Met His Ala 
        275                 280                 285             


Asn Gln Asp Leu Val Thr Gly Tyr Leu Lys Asp Thr Leu Lys Phe Lys 
    290                 295                 300                 


Gly Phe Val Ile Ser Asp Trp Glu Gly Ile Asp Arg Ile Thr Thr Pro 
305                 310                 315                 320 


Ala Gly Ser Asp Tyr Ser Tyr Ser Val Lys Ala Ser Ile Leu Ala Gly 
                325                 330                 335     


Leu Asp Met Ile Met Val Pro Asn Asn Tyr Gln Gln Phe Ile Ser Ile 
            340                 345                 350         


Leu Thr Gly His Val Asn Gly Gly Val Ile Pro Met Ser Arg Ile Asp 
        355                 360                 365             


Asp Ala Val Thr Arg Ile Leu Arg Val Lys Phe Thr Met Gly Leu Phe 
    370                 375                 380                 


Glu Asn Pro Tyr Ala Asp Pro Ala Met Ala Glu Gln Leu Gly Lys Gln 
385                 390                 395                 400 


Glu His Arg Asp Leu Ala Arg Glu Ala Ala Arg Lys Ser Leu Val Leu 
                405                 410                 415     


Leu Lys Asn Gly Lys Thr Ser Thr Asp Ala Pro Leu Leu Pro Leu Pro 
            420                 425                 430         


Lys Lys Ala Pro Lys Ile Leu Val Ala Gly Ser His Ala Asp Asn Leu 
        435                 440                 445             


Gly Tyr Gln Cys Gly Gly Trp Thr Ile Glu Trp Gln Gly Asp Thr Gly 
    450                 455                 460                 


Arg Thr Thr Val Gly Thr Thr Ile Leu Glu Ala Val Lys Ala Ala Val 
465                 470                 475                 480 


Asp Pro Ser Thr Val Val Val Phe Ala Glu Asn Pro Asp Ala Glu Phe 
                485                 490                 495     


Val Lys Ser Gly Gly Phe Ser Tyr Ala Ile Val Ala Val Gly Glu His 
            500                 505                 510         


Pro Tyr Thr Glu Thr Lys Gly Asp Asn Leu Asn Leu Thr Ile Pro Glu 
        515                 520                 525             


Pro Gly Leu Ser Thr Val Gln Ala Val Cys Gly Gly Val Arg Cys Ala 
    530                 535                 540                 


Thr Val Leu Ile Ser Gly Arg Pro Val Val Val Gln Pro Leu Leu Ala 
545                 550                 555                 560 


Ala Ser Asp Ala Leu Val Ala Ala Trp Leu Pro Gly Ser Glu Gly Gln 
                565                 570                 575     


Gly Val Thr Asp Ala Leu Phe Gly Asp Phe Gly Phe Thr Gly Arg Leu 
            580                 585                 590         


Pro Arg Thr Trp Phe Lys Ser Val Asp Gln Leu Pro Met Asn Val Gly 
        595                 600                 605             


Asp Ala His Tyr Asp Pro Leu Phe Arg Leu Gly Tyr Gly Leu Thr Thr 
    610                 615                 620                 


Asn Ala Thr Lys Lys Tyr 
625                 630 


