                         SEQUENCE LISTING

<110>  National Research Council of Canada
       Hussack, Greg
       Arbabi-Ghahroudi, Mehdi
       Mackenzie, Roger
       Tanha, Jamshid
 
<120>  CLOSTRIDIUM DIFFICILE-SPECIFIC ANTIBODIES AND USES THEREOF

<130>  12212-98

<140>  PCT/CA2011/001201
<141>  2011-10-25

<150>  61/406,254
<151>  2010-10-25

<160>  87    

<170>  PatentIn version 3.5

<210>  1
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR1

<400>  1

Gly Arg Thr Phe Asn Thr Leu Ser 
1               5               


<210>  2
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR1

<400>  2

Gly Arg Thr Phe Ser Met Tyr Arg 
1               5               


<210>  3
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR1

<400>  3

Gly Arg Thr Leu Ser Ser Tyr Ile 
1               5               


<210>  4
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR1

<400>  4

Gly Arg Thr Phe Ser Met Asp Pro 
1               5               


<210>  5
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR1

<400>  5

Ile Arg Ser Phe Ser Asn Arg Asn 
1               5               


<210>  6
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR1

<400>  6

Glu Arg Thr Phe Ser Arg Tyr Pro 
1               5               


<210>  7
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR2

<400>  7

Val Ser Arg Ser Gly Gly Ser Thr 
1               5               


<210>  8
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR2

<400>  8

Ile Thr Arg Asn Gly Ser Ser Thr 
1               5               


<210>  9
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR2

<400>  9

Ile Ser Arg Arg Gly Gly Asn Ser 
1               5               


<210>  10
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR2

<400>  10

Gly Ser Ser Thr Gly Arg Thr Thr 
1               5               


<210>  11
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR2

<400>  11

Ile Ser Trp Gly Gly Gly Ser Thr 
1               5               


<210>  12
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR2

<400>  12

Ile Ser Ser Thr Gly Thr Ser Thr 
1               5               


<210>  13
<211>  18
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR3

<400>  13

Ala Ala Ala Ala Thr Lys Ser Asn Thr Thr Ala Tyr Arg Leu Ser Phe 
1               5                   10                  15      


Asp Tyr 
        


<210>  14
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR3

<400>  14

Ala Ala Thr Ser Gly Ser Ser Tyr Leu Asp Ala Ala His Val Tyr Asp 
1               5                   10                  15      


Tyr 
    


<210>  15
<211>  21
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR3

<400>  15

Ala Ala Asp Gly Ser Val Ala Gly Trp Gly Arg Arg Ser Val Ser Val 
1               5                   10                  15      


Ser Ser Tyr Asp Tyr 
            20      


<210>  16
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR3

<400>  16

Ala Ala Ala Pro Tyr Gly Ala Asn Trp Tyr Arg Asp Glu Tyr Ala Tyr 
1               5                   10                  15      


<210>  17
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR3

<400>  17

Ala Ala Glu Phe Gly His Asn Ile Ala Thr Ser Ser Asp Glu Tyr Asp 
1               5                   10                  15      


Tyr 
    


<210>  18
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdA CDR3

<400>  18

Ala Val Asn Ser Gln Arg Thr Arg Leu Gln Asp Pro Asn Glu Tyr Asp 
1               5                   10                  15      


Tyr 
    


<210>  19
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdB CDR1

<400>  19

Gly Asn Ile Phe Ser Ile Asn Thr 
1               5               


<210>  20
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdB CDR1

<400>  20

Gly Arg Thr Ala Ser Gly Tyr Gly 
1               5               


<210>  21
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdB CDR1

<400>  21

Gly Arg Thr Phe Ser Ser Gly Val 
1               5               


<210>  22
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdB CDR1

<400>  22

Gly Leu Ser Arg Tyr Ala 
1               5       


<210>  23
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdB CDR1

<400>  23

Gly Ser Ile Ser Arg Ile Ser Thr 
1               5               


<210>  24
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdB CDR2

<400>  24

Ile Thr Ser Gly Gly Thr Thr 
1               5           


<210>  25
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdB CDR2

<400>  25

Ile Ser Arg Ser Gly Ala Gly Thr 
1               5               


<210>  26
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdB CDR2

<400>  26

Ile Thr Thr Gly Gly Ser Thr 
1               5           


<210>  27
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdB CDR2

<400>  27

Thr Asn Trp Ser Ser Gly Asn Thr 
1               5               


<210>  28
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdB CDR2

<400>  28

Ile Ser Thr Gly Gly Thr Thr 
1               5           


<210>  29
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdB CDR3

<400>  29

Asn Thr Val Lys Val Val Gly Gly Arg Leu Asp Asn Pro Asp Tyr 
1               5                   10                  15  


<210>  30
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdB CDR3

<400>  30

Val Ala Arg Pro Thr Lys Val Asp Arg Asp Tyr Ala Thr Arg Arg Glu 
1               5                   10                  15      


Met Tyr Asn Tyr 
            20  


<210>  31
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdB CDR3

<400>  31

Asn Ser Val Ala Val Val Gly Gly Val Ile Lys Ser Pro Asp Tyr 
1               5                   10                  15  


<210>  32
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdB CDR3

<400>  32

Ala Ala Arg Lys Leu Asp Val Pro Ser Arg Tyr Ser Gln His Tyr Asp 
1               5                   10                  15      


Tyr 
    


<210>  33
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  anti-TcdB CDR3

<400>  33

Ala Ala Gly Trp Lys Val Val Arg Gly Ser Leu Glu Tyr Glu Tyr 
1               5                   10                  15  


<210>  34
<211>  125
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdA sdAb

<400>  34

Gln Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Asn Thr Leu 
            20                  25                  30          


Ser Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Ala Val Ser Arg Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Ala Ala Thr Lys Ser Asn Thr Thr Ala Tyr Arg Leu Ser Phe 
            100                 105                 110         


Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125 


<210>  35
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdA sdAb

<400>  35

Gln Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Met Tyr 
            20                  25                  30          


Arg Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Gly Val Ile Thr Arg Asn Gly Ser Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Thr Ser Gly Ser Ser Tyr Leu Asp Ala Ala His Val Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  36
<211>  128
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdA sdAb

<400>  36

Gln Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Leu Ser Ser Tyr 
            20                  25                  30          


Ile Val Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Ser Arg Arg Gly Gly Asn Ser Ala Tyr Val Glu Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Asp Gly Ser Val Ala Gly Trp Gly Arg Arg Ser Val Ser Val 
            100                 105                 110         


Ser Ser Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125             


<210>  37
<211>  123
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdA sdAb

<400>  37

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ala Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Met Asp 
            20                  25                  30          


Pro Met Ala Trp Phe Arg Gln Pro Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Ala Gly Ser Ser Thr Gly Arg Thr Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Ala Pro Tyr Gly Ala Asn Trp Tyr Arg Asp Glu Tyr Ala Tyr 
            100                 105                 110         


Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120             


<210>  38
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdA sdAb

<400>  38

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Ile Arg Ser Phe Ser Asn Arg 
            20                  25                  30          


Asn Met Gly Trp Phe Arg Gln Pro Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Ile Ser Trp Gly Gly Gly Ser Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Lys Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Glu Phe Gly His Asn Ile Ala Thr Ser Ser Asp Glu Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  39
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdA sdAb

<400>  39

Gln Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Glu Arg Thr Phe Ser Arg Tyr 
            20                  25                  30          


Pro Val Ala Trp Phe Arg Gln Ala Pro Gly Ala Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Val Ile Ser Ser Thr Gly Thr Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Val Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asn Leu Lys Arg Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Val Asn Ser Gln Arg Thr Arg Leu Gln Asp Pro Asn Glu Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  40
<211>  121
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdB sdAb

<400>  40

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Asn Ile Phe Ser Ile Asn 
            20                  25                  30          


Thr Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Leu Glu Leu Val 
        35                  40                  45              


Ala Ala Ile Thr Ser Gly Gly Thr Thr Ser Tyr Thr Asp Ser Val Glu 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ala Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Ala Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Thr Val Lys Val Val Gly Gly Arg Leu Asp Asn Pro Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120     


<210>  41
<211>  127
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdB sdAb

<400>  41

Gln Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ala Ser Gly Tyr 
            20                  25                  30          


Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Ala Ile Ser Arg Ser Gly Ala Gly Thr Leu Asn Ala Asp Phe Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Val Ala Arg Pro Thr Lys Val Asp Arg Asp Tyr Ala Thr Arg Arg Glu 
            100                 105                 110         


Met Tyr Asn Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125         


<210>  42
<211>  121
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdB sdAb

<400>  42

Gln Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ser Ala Ser Gly Arg Thr Phe Ser Ser Gly 
            20                  25                  30          


Val Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Ala Ile Thr Thr Gly Gly Ser Thr Ser Tyr Thr Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ser Val Ala Val Val Gly Gly Val Ile Lys Ser Pro Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120     


<210>  43
<211>  122
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdB sdAb

<400>  43

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Leu Ser Arg Tyr Ala Met 
            20                  25                  30          


Ala Trp Phe Arg Gln Gly Thr Gly Lys Glu Arg Glu Phe Val Ala Ser 
        35                  40                  45              


Thr Asn Trp Ser Ser Gly Asn Thr Pro Tyr Ala Asp Ser Val Lys Gly 
    50                  55                  60                  


Arg Phe Ile Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu Gln 
65                  70                  75                  80  


Met Asn Ser Leu Lys Pro Gly Asp Thr Ala Ile Tyr Tyr Cys Ala Ala 
                85                  90                  95      


Arg Lys Leu Asp Val Pro Ser Arg Tyr Ser Gln His Tyr Asp Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120         


<210>  44
<211>  121
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdB sdAb

<400>  44

Gln Val Gln Leu Val Glu Ser Gly Gly Asp Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Ser Arg Ile Ser 
            20                  25                  30          


Thr Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Thr Ile Ser Thr Gly Gly Thr Thr Asn Tyr Ala Glu Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Val Ser Arg Asp Asn Ala Lys Asn Thr Met Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Gly Trp Lys Val Val Arg Gly Ser Leu Glu Tyr Glu Tyr Ser Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120     


<210>  45
<211>  125
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdA sdAb

<400>  45

Gln Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Asn Thr Leu 
            20                  25                  30          


Ser Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Cys Ala Val Ser Arg Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Cys Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Ala Ala Thr Lys Ser Asn Thr Thr Ala Tyr Arg Leu Ser Phe 
            100                 105                 110         


Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125 


<210>  46
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdA sdAb

<400>  46

Gln Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Met Tyr 
            20                  25                  30          


Arg Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Cys Val Ile Thr Arg Asn Gly Ser Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Cys Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Thr Ser Gly Ser Ser Tyr Leu Asp Ala Ala His Val Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  47
<211>  128
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdA sdAb

<400>  47

Gln Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Leu Ser Ser Tyr 
            20                  25                  30          


Ile Val Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Cys Gly Ile Ser Arg Arg Gly Gly Asn Ser Ala Tyr Val Glu Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Cys Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Asp Gly Ser Val Ala Gly Trp Gly Arg Arg Ser Val Ser Val 
            100                 105                 110         


Ser Ser Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125             


<210>  48
<211>  123
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdA sdAb

<400>  48

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ala Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Met Asp 
            20                  25                  30          


Pro Met Ala Trp Phe Arg Gln Pro Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Cys Ala Gly Ser Ser Thr Gly Arg Thr Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Cys Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Ala Pro Tyr Gly Ala Asn Trp Tyr Arg Asp Glu Tyr Ala Tyr 
            100                 105                 110         


Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120             


<210>  49
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdA sdAb

<400>  49

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Ile Arg Ser Phe Ser Asn Arg 
            20                  25                  30          


Asn Met Gly Trp Phe Arg Gln Pro Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Cys Gly Ile Ser Trp Gly Gly Gly Ser Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Cys Ser Arg Asp Asn Ala Lys Lys Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Glu Phe Gly His Asn Ile Ala Thr Ser Ser Asp Glu Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  50
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdA sdAb

<400>  50

Gln Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Glu Arg Thr Phe Ser Arg Tyr 
            20                  25                  30          


Pro Val Ala Trp Phe Arg Gln Ala Pro Gly Ala Glu Arg Glu Phe Val 
        35                  40                  45              


Cys Val Ile Ser Ser Thr Gly Thr Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Cys Ser Arg Asp Asn Ala Lys Val Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asn Leu Lys Arg Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Val Asn Ser Gln Arg Thr Arg Leu Gln Asp Pro Asn Glu Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  51
<211>  121
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdB sdAb

<400>  51

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Asn Ile Phe Ser Ile Asn 
            20                  25                  30          


Thr Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Leu Glu Leu Val 
        35                  40                  45              


Cys Ala Ile Thr Ser Gly Gly Thr Thr Ser Tyr Thr Asp Ser Val Glu 
    50                  55                  60                  


Gly Arg Phe Thr Cys Ser Arg Asp Asn Ala Lys Asn Ala Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Ala Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Thr Val Lys Val Val Gly Gly Arg Leu Asp Asn Pro Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120     


<210>  52
<211>  127
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdB sdAb

<400>  52

Gln Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ala Ser Gly Tyr 
            20                  25                  30          


Gly Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Cys Ala Ile Ser Arg Ser Gly Ala Gly Thr Leu Asn Ala Asp Phe Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Cys Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Val Ala Arg Pro Thr Lys Val Asp Arg Asp Tyr Ala Thr Arg Arg Glu 
            100                 105                 110         


Met Tyr Asn Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125         


<210>  53
<211>  121
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdB sdAb

<400>  53

Gln Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ser Ala Ser Gly Arg Thr Phe Ser Ser Gly 
            20                  25                  30          


Val Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Cys Ala Ile Thr Thr Gly Gly Ser Thr Ser Tyr Thr Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Cys Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ser Val Ala Val Val Gly Gly Val Ile Lys Ser Pro Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120     


<210>  54
<211>  122
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdB sdAb

<400>  54

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Leu Ser Arg Tyr Ala Met 
            20                  25                  30          


Ala Trp Phe Arg Gln Gly Thr Gly Lys Glu Arg Glu Phe Val Cys Ser 
        35                  40                  45              


Thr Asn Trp Ser Ser Gly Asn Thr Pro Tyr Ala Asp Ser Val Lys Gly 
    50                  55                  60                  


Arg Phe Ile Cys Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu Gln 
65                  70                  75                  80  


Met Asn Ser Leu Lys Pro Gly Asp Thr Ala Ile Tyr Tyr Cys Ala Ala 
                85                  90                  95      


Arg Lys Leu Asp Val Pro Ser Arg Tyr Ser Gln His Tyr Asp Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120         


<210>  55
<211>  120
<212>  PRT
<213>  Artificial

<220>
<223>  Anti-TcdB sdAb

<400>  55

Gln Val Gln Leu Val Glu Ser Gly Gly Asp Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Ser Arg Ile Ser 
            20                  25                  30          


Thr Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Cys 
        35                  40                  45              


Ala Thr Ile Ser Thr Gly Gly Thr Thr Asn Tyr Ala Glu Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Cys Ser Arg Asp Asn Ala Lys Asn Thr Met Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Gly Trp Lys Val Val Arg Gly Ser Leu Glu Tyr Glu Tyr Ser Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser 
        115                 120 


<210>  56
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  Primer MJ1

<400>  56
gcccagccgg ccatggccsm kgtgcagctg gtggaktctg gggga                       45


<210>  57
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  Primer MJ2

<400>  57
cagccggcca tggcccaggt aaagctggag gagtctgggg ga                          42


<210>  58
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  Primer MJ3

<400>  58
gcccagccgg ccatggccca ggctcaggta cagctggtgg agtct                       45


<210>  59
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Primer CH2

<400>  59
chcgccatca aggtaccagt tga                                               23


<210>  60
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Primer CH2b3

<400>  60
chbggtacct gtcatccacg gaccagctga                                        30


<210>  61
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  Primer MJ7

<400>  61
catgtgtaga ctcgcggccc agccggccat ggcc                                   34


<210>  62
<211>  47
<212>  DNA
<213>  Artificial

<220>
<223>  Primer MJ8

<400>  62
catgtgtaga ttcctggccg gcctggcctg aggagacggt gacctgg                     47


<210>  63
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Bbsl1-VHH sense primer

<400>  63
tatgaagaca ccaggcccag gtaaagctgg aggagtct                               38


<210>  64
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Bbsl2-VHH sense primer

<400>  64
tatgaagaca ccaggcccag gtgcagctgg tggagtct                               38


<210>  65
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  BamHI-VHH antisense primer

<400>  65
ttgttcggat cctgaggaga cggtgacctg                                        30


<210>  66
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  Primer M13FP

<400>  66
gtaaaacgac ggccagt                                                      17


<210>  67
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  Primer M13RP

<400>  67
caggaaacag ctatgac                                                      17


<210>  68
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer A4.2mR-Cys

<400>  68
agtctgcata gtatgtgcta ccaccactcc ggctaacagc gcaaacaaac tc               52


<210>  69
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer A4.2mF-Cys

<400>  69
tagcacatac tatgcagact ccgtgaaggg ccgattcacc tgctccagag ac               52


<210>  70
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer A5.1mR-Cys

<400>  70
agtctgcata gtatgtgcta ctaccattcc gggtaataac gcatacaaac tc               52


<210>  71
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer A19.2mR-Cys

<400>  71
actctacata ggcactatta ccaccacgcc ggctaatacc gcatacaaac tc               52


<210>  72
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer A19.2mF-Cys

<400>  72
taatagtgcc tatgtagagt ccgtgaaggg ccgattcacc tgctccagag ac               52


<210>  73
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Primer A20.1mSfil-F

<400>  73
accgttgcgc aggcccagcc ggccatggcc caggtacagc                             40


<210>  74
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer A20.1mR-Cys

<400>  74
tgtctgcata gtatgtggtc cgccccgtag aactccccgc gcatacaaac tc               52


<210>  75
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer A20.1 mF-Cys

<400>  75
gaccacatac tatgcagaca gcgtgaaggg ccgattcacc tgctccagag ac               52


<210>  76
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  Primer A20.1mSfil-R

<400>  76
gttcggatcc ctggccggcc tggcctgagg agacggtgac c                           41


<210>  77
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer A24.1mR-Cys

<400>  77
agtctgcata gcgtgtgcta cctccacccc agctaatacc gcatacaaac tc               52


<210>  78
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer A24.1mF-Cys

<400>  78
tagcacacgc tatgcagact ccgtgaaggg ccgattcacc tgctccagag ac               52


<210>  79
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer A26.8mR-Cys

<400>  79
agtctgcata gtatgtgctc gtaccagtcg agctaataac gcatacaaac tc               52


<210>  80
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer A26.8mF-Cys

<400>  80
gagcacatac tatgcagact cggtgaaggg ccggttcacc tgctccagag ac               52


<210>  81
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  Tryptic peptide

<400>  81

Glu Phe Val Cys Ala Val Ser Arg 
1               5               


<210>  82
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  Tryptic peptide

<400>  82

Glu Phe Val Cys Val Ile Thr Arg 
1               5               


<210>  83
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  Tryptic peptide

<400>  83

Glu Phe Val Cys Gly Ile Ser Arg 
1               5               


<210>  84
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  Tryptic peptide

<400>  84

Glu Phe Val Cys Ala Gly Ser Ser Thr Gly Arg 
1               5                   10      


<210>  85
<211>  14
<212>  PRT
<213>  Artificial

<220>
<223>  Tryptic peptide

<400>  85

Glu Phe Val Cys Gly Ile Ser Trp Gly Gly Gly Ser Thr Arg 
1               5                   10                  


<210>  86
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  Tryptic peptide

<400>  86

Glu Phe Val Cys Val Ile Ser Ser Thr Gly Thr Ser Thr Tyr Tyr Ala 
1               5                   10                  15      


Asp Ser Val Lys 
            20  


<210>  87
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Tryptic peptide

<400>  87

Phe Thr Cys Ser Arg 
1               5   


