                         SEQUENCE LISTING

<110>  New York Blood Center, Inc.
       Du, Lanying
       Jiang, Shibo
       Zhou, Yusen
 
<120>  Influenza Hemagglutinin-Specific Monoclonal Antibodies for 
       Preventing and Treating Influenza Virus Infection

<130>  1958427-00177

<150>  US 61/405,100
<151>  2010-10-20

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  567
<212>  PRT
<213>  Influenza A virus

<400>  1

Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Ser 
1               5                   10                  15      


Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
            20                  25                  30          


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
        35                  40                  45              


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys 
    50                  55                  60                  


Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
65                  70                  75                  80  


Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val 
                85                  90                  95      


Glu Lys Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn 
            100                 105                 110         


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
        115                 120                 125             


Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser 
    130                 135                 140                 


Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Thr Pro Ser Phe Phe 
145                 150                 155                 160 


Arg Asn Val Val Trp Leu Ile Lys Lys Asn Asn Thr Tyr Pro Thr Ile 
                165                 170                 175     


Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Ile Leu Trp 
            180                 185                 190         


Gly Ile His His Ser Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln 
        195                 200                 205             


Asn Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg 
    210                 215                 220                 


Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Asn Gly 
225                 230                 235                 240 


Arg Met Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn 
                245                 250                 255     


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile 
            260                 265                 270         


Val Lys Lys Gly Asp Ser Ala Ile Val Lys Ser Glu Val Glu Tyr Gly 
        275                 280                 285             


Asn Cys Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser 
    290                 295                 300                 


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
305                 310                 315                 320 


Tyr Val Lys Ser Asn Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Ser 
                325                 330                 335     


Pro Leu Arg Glu Arg Arg Arg Lys Arg Gly Leu Phe Gly Ala Ile Ala 
            340                 345                 350         


Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr Gly 
        355                 360                 365             


Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu 
    370                 375                 380                 


Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser Ile 
385                 390                 395                 400 


Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe Asn 
                405                 410                 415     


Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp Gly 
            420                 425                 430         


Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu 
        435                 440                 445             


Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr 
    450                 455                 460                 


Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn 
465                 470                 475                 480 


Gly Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Glu Ser 
                485                 490                 495     


Val Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala Arg 
            500                 505                 510         


Leu Lys Arg Glu Glu Ile Ser Gly Val Lys Leu Glu Ser Ile Gly Thr 
        515                 520                 525             


Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala Leu 
    530                 535                 540                 


Ala Ile Met Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly Ser 
545                 550                 555                 560 


Leu Gln Cys Arg Ile Cys Ile 
                565         


<210>  2
<211>  320
<212>  PRT
<213>  Influenza A virus

<400>  2

Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val Asp Thr 
1               5                   10                  15      


Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile Leu Glu 
            20                  25                  30          


Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys Pro Leu 
        35                  40                  45              


Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn Pro Met 
    50                  55                  60                  


Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val Glu Lys 
65                  70                  75                  80  


Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn Asp Tyr 
                85                  90                  95      


Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu Lys Ile 
            100                 105                 110         


Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser Ser Gly 
        115                 120                 125             


Val Ser Ser Ala Cys Pro Tyr Gln Gly Thr Pro Ser Phe Phe Arg Asn 
    130                 135                 140                 


Val Val Trp Leu Ile Lys Lys Asn Asn Thr Tyr Pro Thr Ile Lys Arg 
145                 150                 155                 160 


Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Ile Leu Trp Gly Ile 
                165                 170                 175     


His His Ser Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln Asn Pro 
            180                 185                 190         


Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg Leu Val 
        195                 200                 205             


Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Asn Gly Arg Met 
    210                 215                 220                 


Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu 
225                 230                 235                 240 


Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys 
                245                 250                 255     


Lys Gly Asp Ser Ala Ile Val Lys Ser Glu Val Glu Tyr Gly Asn Cys 
            260                 265                 270         


Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser Met Pro 
        275                 280                 285             


Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val 
    290                 295                 300                 


Lys Ser Asn Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Ser Pro Leu 
305                 310                 315                 320 


<210>  3
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fodon linker sequence

<400>  3

Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys 
1               5                   10                  15      


Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu 
            20                  25          


<210>  4
<211>  229
<212>  PRT
<213>  Homo sapiens

<400>  4

Arg Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
1               5                   10                  15      


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
            100                 105                 110         


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Pro Gly Lys 
225                 


<210>  5
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A/Anhui/1/2005(H5N1) HA1(+3-322)-Fd-hFc fusion protein

<400>  5

Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val Asp Thr 
1               5                   10                  15      


Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile Leu Glu 
            20                  25                  30          


Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys Pro Leu 
        35                  40                  45              


Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn Pro Met 
    50                  55                  60                  


Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val Glu Lys 
65                  70                  75                  80  


Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn Asp Tyr 
                85                  90                  95      


Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu Lys Ile 
            100                 105                 110         


Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser Ser Gly 
        115                 120                 125             


Val Ser Ser Ala Cys Pro Tyr Gln Gly Thr Pro Ser Phe Phe Arg Asn 
    130                 135                 140                 


Val Val Trp Leu Ile Lys Lys Asn Asn Thr Tyr Pro Thr Ile Lys Arg 
145                 150                 155                 160 


Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Ile Leu Trp Gly Ile 
                165                 170                 175     


His His Ser Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln Asn Pro 
            180                 185                 190         


Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg Leu Val 
        195                 200                 205             


Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Asn Gly Arg Met 
    210                 215                 220                 


Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu 
225                 230                 235                 240 


Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys 
                245                 250                 255     


Lys Gly Asp Ser Ala Ile Val Lys Ser Glu Val Glu Tyr Gly Asn Cys 
            260                 265                 270         


Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser Met Pro 
        275                 280                 285             


Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val 
    290                 295                 300                 


Lys Ser Asn Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Ser Pro Leu 
305                 310                 315                 320 


Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys 
                325                 330                 335     


Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu Arg Ser Asp Lys Thr 
            340                 345                 350         


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
        355                 360                 365             


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
    370                 375                 380                 


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
385                 390                 395                 400 


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
                405                 410                 415     


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
            420                 425                 430         


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
        435                 440                 445             


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
    450                 455                 460                 


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
465                 470                 475                 480 


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
                485                 490                 495     


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
            500                 505                 510         


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
        515                 520                 525             


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
    530                 535                 540                 


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
545                 550                 555                 560 


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                565                 570                 575     


<210>  6
<211>  549
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A/Anhui/1/2005(H5N1) HA1(+3-322)-hFc fusion protein

<400>  6

Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val Asp Thr 
1               5                   10                  15      


Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile Leu Glu 
            20                  25                  30          


Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys Pro Leu 
        35                  40                  45              


Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn Pro Met 
    50                  55                  60                  


Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val Glu Lys 
65                  70                  75                  80  


Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn Asp Tyr 
                85                  90                  95      


Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu Lys Ile 
            100                 105                 110         


Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser Ser Gly 
        115                 120                 125             


Val Ser Ser Ala Cys Pro Tyr Gln Gly Thr Pro Ser Phe Phe Arg Asn 
    130                 135                 140                 


Val Val Trp Leu Ile Lys Lys Asn Asn Thr Tyr Pro Thr Ile Lys Arg 
145                 150                 155                 160 


Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Ile Leu Trp Gly Ile 
                165                 170                 175     


His His Ser Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln Asn Pro 
            180                 185                 190         


Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg Leu Val 
        195                 200                 205             


Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Asn Gly Arg Met 
    210                 215                 220                 


Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu 
225                 230                 235                 240 


Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys 
                245                 250                 255     


Lys Gly Asp Ser Ala Ile Val Lys Ser Glu Val Glu Tyr Gly Asn Cys 
            260                 265                 270         


Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser Met Pro 
        275                 280                 285             


Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val 
    290                 295                 300                 


Lys Ser Asn Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Ser Pro Leu 
305                 310                 315                 320 


Arg Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
                325                 330                 335     


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            340                 345                 350         


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        355                 360                 365             


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
    370                 375                 380                 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
385                 390                 395                 400 


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                405                 410                 415     


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
            420                 425                 430         


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        435                 440                 445             


Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln 
    450                 455                 460                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
465                 470                 475                 480 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                485                 490                 495     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
            500                 505                 510         


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
        515                 520                 525             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    530                 535                 540                 


Leu Ser Pro Gly Lys 
545                 


<210>  7
<211>  347
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A/Anhui/1/2005(H5N1) HA1(+3-322)-Fd fusion protein

<400>  7

Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val Asp Thr 
1               5                   10                  15      


Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile Leu Glu 
            20                  25                  30          


Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys Pro Leu 
        35                  40                  45              


Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn Pro Met 
    50                  55                  60                  


Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val Glu Lys 
65                  70                  75                  80  


Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn Asp Tyr 
                85                  90                  95      


Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu Lys Ile 
            100                 105                 110         


Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser Ser Gly 
        115                 120                 125             


Val Ser Ser Ala Cys Pro Tyr Gln Gly Thr Pro Ser Phe Phe Arg Asn 
    130                 135                 140                 


Val Val Trp Leu Ile Lys Lys Asn Asn Thr Tyr Pro Thr Ile Lys Arg 
145                 150                 155                 160 


Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Ile Leu Trp Gly Ile 
                165                 170                 175     


His His Ser Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln Asn Pro 
            180                 185                 190         


Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg Leu Val 
        195                 200                 205             


Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Asn Gly Arg Met 
    210                 215                 220                 


Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu 
225                 230                 235                 240 


Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys 
                245                 250                 255     


Lys Gly Asp Ser Ala Ile Val Lys Ser Glu Val Glu Tyr Gly Asn Cys 
            260                 265                 270         


Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser Met Pro 
        275                 280                 285             


Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val 
    290                 295                 300                 


Lys Ser Asn Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Ser Pro Leu 
305                 310                 315                 320 


Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys 
                325                 330                 335     


Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu 
            340                 345         


<210>  8
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A/Anhui/1/2005(H5N1) HA1(+3-322)-His fusion protein

<400>  8

Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val Asp Thr 
1               5                   10                  15      


Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile Leu Glu 
            20                  25                  30          


Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys Pro Leu 
        35                  40                  45              


Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn Pro Met 
    50                  55                  60                  


Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val Glu Lys 
65                  70                  75                  80  


Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn Asp Tyr 
                85                  90                  95      


Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu Lys Ile 
            100                 105                 110         


Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser Ser Gly 
        115                 120                 125             


Val Ser Ser Ala Cys Pro Tyr Gln Gly Thr Pro Ser Phe Phe Arg Asn 
    130                 135                 140                 


Val Val Trp Leu Ile Lys Lys Asn Asn Thr Tyr Pro Thr Ile Lys Arg 
145                 150                 155                 160 


Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Ile Leu Trp Gly Ile 
                165                 170                 175     


His His Ser Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln Asn Pro 
            180                 185                 190         


Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg Leu Val 
        195                 200                 205             


Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Asn Gly Arg Met 
    210                 215                 220                 


Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu 
225                 230                 235                 240 


Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys 
                245                 250                 255     


Lys Gly Asp Ser Ala Ile Val Lys Ser Glu Val Glu Tyr Gly Asn Cys 
            260                 265                 270         


Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser Met Pro 
        275                 280                 285             


Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val 
    290                 295                 300                 


Lys Ser Asn Lys Leu Val Leu Ala Thr Gly Leu Arg Asn Ser Pro Leu 
305                 310                 315                 320 


His His His His His His 
                325     


<210>  9
<211>  218
<212>  PRT
<213>  Influenza A virus

<400>  9

Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys Ser 
1               5                   10                  15      


Ser Trp Ser Asp His Glu Ala Ser Ser Gly Val Ser Ser Ala Cys Pro 
            20                  25                  30          


Tyr Gln Gly Thr Pro Ser Phe Phe Arg Asn Val Val Trp Leu Ile Lys 
        35                  40                  45              


Lys Asn Asn Thr Tyr Pro Thr Ile Lys Arg Ser Tyr Asn Asn Thr Asn 
    50                  55                  60                  


Gln Glu Asp Leu Leu Ile Leu Trp Gly Ile His His Ser Asn Asp Ala 
65                  70                  75                  80  


Ala Glu Gln Thr Lys Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser Val 
                85                  90                  95      


Gly Thr Ser Thr Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr Arg 
            100                 105                 110         


Ser Lys Val Asn Gly Gln Asn Gly Arg Met Asp Phe Phe Trp Thr Ile 
        115                 120                 125             


Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile 
    130                 135                 140                 


Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys Lys Gly Asp Ser Ala Ile 
145                 150                 155                 160 


Val Lys Ser Glu Val Glu Tyr Gly Asn Cys Asn Thr Lys Cys Gln Thr 
                165                 170                 175     


Pro Ile Gly Ala Ile Asn Ser Ser Met Pro Phe His Asn Ile His Pro 
            180                 185                 190         


Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val Lys Ser Asn Lys Leu Val 
        195                 200                 205             


Leu Ala Thr Gly Leu Arg Asn Ser Pro Leu 
    210                 215             


<210>  10
<211>  155
<212>  PRT
<213>  Influenza A virus

<400>  10

Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys Ser 
1               5                   10                  15      


Ser Trp Ser Asp His Glu Ala Ser Ser Gly Val Ser Ser Ala Cys Pro 
            20                  25                  30          


Tyr Gln Gly Thr Pro Ser Phe Phe Arg Asn Val Val Trp Leu Ile Lys 
        35                  40                  45              


Lys Asn Asn Thr Tyr Pro Thr Ile Lys Arg Ser Tyr Asn Asn Thr Asn 
    50                  55                  60                  


Gln Glu Asp Leu Leu Ile Leu Trp Gly Ile His His Ser Asn Asp Ala 
65                  70                  75                  80  


Ala Glu Gln Thr Lys Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser Val 
                85                  90                  95      


Gly Thr Ser Thr Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr Arg 
            100                 105                 110         


Ser Lys Val Asn Gly Gln Asn Gly Arg Met Asp Phe Phe Trp Thr Ile 
        115                 120                 125             


Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile 
    130                 135                 140                 


Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys Lys 
145                 150                 155 


<210>  11
<211>  257
<212>  PRT
<213>  Influenza A virus

<400>  11

Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val Asp Thr 
1               5                   10                  15      


Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile Leu Glu 
            20                  25                  30          


Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys Pro Leu 
        35                  40                  45              


Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn Pro Met 
    50                  55                  60                  


Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val Glu Lys 
65                  70                  75                  80  


Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn Asp Tyr 
                85                  90                  95      


Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu Lys Ile 
            100                 105                 110         


Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser Ser Gly 
        115                 120                 125             


Val Ser Ser Ala Cys Pro Tyr Gln Gly Thr Pro Ser Phe Phe Arg Asn 
    130                 135                 140                 


Val Val Trp Leu Ile Lys Lys Asn Asn Thr Tyr Pro Thr Ile Lys Arg 
145                 150                 155                 160 


Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Ile Leu Trp Gly Ile 
                165                 170                 175     


His His Ser Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln Asn Pro 
            180                 185                 190         


Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg Leu Val 
        195                 200                 205             


Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Asn Gly Arg Met 
    210                 215                 220                 


Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu 
225                 230                 235                 240 


Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys 
                245                 250                 255     


Lys 
    


<210>  12
<211>  232
<212>  PRT
<213>  Influenza A virus

<400>  12

His Ala Gln Asp Ile Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp 
1               5                   10                  15      


Leu Asp Gly Val Lys Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly 
            20                  25                  30          


Trp Leu Leu Gly Asn Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu 
        35                  40                  45              


Trp Ser Tyr Ile Val Glu Lys Ala Asn Pro Ala Asn Asp Leu Cys Tyr 
    50                  55                  60                  


Pro Gly Asn Phe Asn Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg 
65                  70                  75                  80  


Ile Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser 
                85                  90                  95      


Asp His Glu Ala Ser Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly 
            100                 105                 110         


Thr Pro Ser Phe Phe Arg Asn Val Val Trp Leu Ile Lys Lys Asn Asn 
        115                 120                 125             


Thr Tyr Pro Thr Ile Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp 
    130                 135                 140                 


Leu Leu Ile Leu Trp Gly Ile His His Ser Asn Asp Ala Ala Glu Gln 
145                 150                 155                 160 


Thr Lys Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser 
                165                 170                 175     


Thr Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val 
            180                 185                 190         


Asn Gly Gln Asn Gly Arg Met Asp Phe Phe Trp Thr Ile Leu Lys Pro 
        195                 200                 205             


Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu 
    210                 215                 220                 


Tyr Ala Tyr Lys Ile Val Lys Lys 
225                 230         


<210>  13
<211>  215
<212>  PRT
<213>  Influenza A virus

<400>  13

Asp Gly Val Lys Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp 
1               5                   10                  15      


Leu Leu Gly Asn Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp 
            20                  25                  30          


Ser Tyr Ile Val Glu Lys Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro 
        35                  40                  45              


Gly Asn Phe Asn Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile 
    50                  55                  60                  


Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp 
65                  70                  75                  80  


His Glu Ala Ser Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Thr 
                85                  90                  95      


Pro Ser Phe Phe Arg Asn Val Val Trp Leu Ile Lys Lys Asn Asn Thr 
            100                 105                 110         


Tyr Pro Thr Ile Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu 
        115                 120                 125             


Leu Ile Leu Trp Gly Ile His His Ser Asn Asp Ala Ala Glu Gln Thr 
    130                 135                 140                 


Lys Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr 
145                 150                 155                 160 


Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn 
                165                 170                 175     


Gly Gln Asn Gly Arg Met Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn 
            180                 185                 190         


Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr 
        195                 200                 205             


Ala Tyr Lys Ile Val Lys Lys 
    210                 215 


<210>  14
<211>  188
<212>  PRT
<213>  Influenza A virus

<400>  14

Asn Val Pro Glu Trp Ser Tyr Ile Val Glu Lys Ala Asn Pro Ala Asn 
1               5                   10                  15      


Asp Leu Cys Tyr Pro Gly Asn Phe Asn Asp Tyr Glu Glu Leu Lys His 
            20                  25                  30          


Leu Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys 
        35                  40                  45              


Ser Ser Trp Ser Asp His Glu Ala Ser Ser Gly Val Ser Ser Ala Cys 
    50                  55                  60                  


Pro Tyr Gln Gly Thr Pro Ser Phe Phe Arg Asn Val Val Trp Leu Ile 
65                  70                  75                  80  


Lys Lys Asn Asn Thr Tyr Pro Thr Ile Lys Arg Ser Tyr Asn Asn Thr 
                85                  90                  95      


Asn Gln Glu Asp Leu Leu Ile Leu Trp Gly Ile His His Ser Asn Asp 
            100                 105                 110         


Ala Ala Glu Gln Thr Lys Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser 
        115                 120                 125             


Val Gly Thr Ser Thr Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr 
    130                 135                 140                 


Arg Ser Lys Val Asn Gly Gln Asn Gly Arg Met Asp Phe Phe Trp Thr 
145                 150                 155                 160 


Ile Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe 
                165                 170                 175     


Ile Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys Lys 
            180                 185             


<210>  15
<211>  44
<212>  PRT
<213>  Influenza A virus

<400>  15

Asn Val Pro Glu Trp Ser Tyr Ile Val Glu Lys Ala Asn Pro Ala Asn 
1               5                   10                  15      


Asp Leu Cys Tyr Pro Gly Asn Phe Asn Asp Tyr Glu Glu Leu Lys His 
            20                  25                  30          


Leu Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln 
        35                  40                  


<210>  16
<211>  474
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A/Anhui/1/2005(H5N1) HA1(+105-322)-Fd-hFc fusion protein

<400>  16

Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys Ser 
1               5                   10                  15      


Ser Trp Ser Asp His Glu Ala Ser Ser Gly Val Ser Ser Ala Cys Pro 
            20                  25                  30          


Tyr Gln Gly Thr Pro Ser Phe Phe Arg Asn Val Val Trp Leu Ile Lys 
        35                  40                  45              


Lys Asn Asn Thr Tyr Pro Thr Ile Lys Arg Ser Tyr Asn Asn Thr Asn 
    50                  55                  60                  


Gln Glu Asp Leu Leu Ile Leu Trp Gly Ile His His Ser Asn Asp Ala 
65                  70                  75                  80  


Ala Glu Gln Thr Lys Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser Val 
                85                  90                  95      


Gly Thr Ser Thr Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr Arg 
            100                 105                 110         


Ser Lys Val Asn Gly Gln Asn Gly Arg Met Asp Phe Phe Trp Thr Ile 
        115                 120                 125             


Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile 
    130                 135                 140                 


Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys Lys Gly Asp Ser Ala Ile 
145                 150                 155                 160 


Val Lys Ser Glu Val Glu Tyr Gly Asn Cys Asn Thr Lys Cys Gln Thr 
                165                 170                 175     


Pro Ile Gly Ala Ile Asn Ser Ser Met Pro Phe His Asn Ile His Pro 
            180                 185                 190         


Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val Lys Ser Asn Lys Leu Val 
        195                 200                 205             


Leu Ala Thr Gly Leu Arg Asn Ser Pro Leu Gly Tyr Ile Pro Glu Ala 
    210                 215                 220                 


Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu 
225                 230                 235                 240 


Leu Ser Thr Phe Leu Arg Ser Asp Lys Thr His Thr Cys Pro Pro Cys 
                245                 250                 255     


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
            260                 265                 270         


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
        275                 280                 285             


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
    290                 295                 300                 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
305                 310                 315                 320 


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
                325                 330                 335     


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
            340                 345                 350         


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
        355                 360                 365             


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
    370                 375                 380                 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
385                 390                 395                 400 


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
                405                 410                 415     


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
            420                 425                 430         


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
        435                 440                 445             


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
    450                 455                 460                 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
465                 470                 


<210>  17
<211>  411
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A/Anhui/1/2005(H5N1) HA1(+105-259)-Fd-hFc fusion protein

<400>  17

Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys Ser 
1               5                   10                  15      


Ser Trp Ser Asp His Glu Ala Ser Ser Gly Val Ser Ser Ala Cys Pro 
            20                  25                  30          


Tyr Gln Gly Thr Pro Ser Phe Phe Arg Asn Val Val Trp Leu Ile Lys 
        35                  40                  45              


Lys Asn Asn Thr Tyr Pro Thr Ile Lys Arg Ser Tyr Asn Asn Thr Asn 
    50                  55                  60                  


Gln Glu Asp Leu Leu Ile Leu Trp Gly Ile His His Ser Asn Asp Ala 
65                  70                  75                  80  


Ala Glu Gln Thr Lys Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser Val 
                85                  90                  95      


Gly Thr Ser Thr Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr Arg 
            100                 105                 110         


Ser Lys Val Asn Gly Gln Asn Gly Arg Met Asp Phe Phe Trp Thr Ile 
        115                 120                 125             


Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile 
    130                 135                 140                 


Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys Lys Gly Tyr Ile Pro Glu 
145                 150                 155                 160 


Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val 
                165                 170                 175     


Leu Leu Ser Thr Phe Leu Arg Ser Asp Lys Thr His Thr Cys Pro Pro 
            180                 185                 190         


Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro 
        195                 200                 205             


Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 
    210                 215                 220                 


Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn 
225                 230                 235                 240 


Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 
                245                 250                 255     


Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val 
            260                 265                 270         


Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser 
        275                 280                 285             


Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
    290                 295                 300                 


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu 
305                 310                 315                 320 


Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
                325                 330                 335     


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
            340                 345                 350         


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
        355                 360                 365             


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
    370                 375                 380                 


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
385                 390                 395                 400 


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                405                 410     


<210>  18
<211>  513
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A/Anhui/1/2005(H5N1) HA1(+3-259)-Fd-hFc fusion protein

<400>  18

Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val Asp Thr 
1               5                   10                  15      


Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile Leu Glu 
            20                  25                  30          


Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys Pro Leu 
        35                  40                  45              


Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn Pro Met 
    50                  55                  60                  


Cys Asp Glu Phe Ile Asn Val Pro Glu Trp Ser Tyr Ile Val Glu Lys 
65                  70                  75                  80  


Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn Asp Tyr 
                85                  90                  95      


Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu Lys Ile 
            100                 105                 110         


Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser Ser Gly 
        115                 120                 125             


Val Ser Ser Ala Cys Pro Tyr Gln Gly Thr Pro Ser Phe Phe Arg Asn 
    130                 135                 140                 


Val Val Trp Leu Ile Lys Lys Asn Asn Thr Tyr Pro Thr Ile Lys Arg 
145                 150                 155                 160 


Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Ile Leu Trp Gly Ile 
                165                 170                 175     


His His Ser Asn Asp Ala Ala Glu Gln Thr Lys Leu Tyr Gln Asn Pro 
            180                 185                 190         


Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg Leu Val 
        195                 200                 205             


Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Asn Gly Arg Met 
    210                 215                 220                 


Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu 
225                 230                 235                 240 


Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys 
                245                 250                 255     


Lys Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg 
            260                 265                 270         


Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu Arg Ser Asp Lys 
        275                 280                 285             


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
    290                 295                 300                 


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
305                 310                 315                 320 


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
                325                 330                 335     


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
            340                 345                 350         


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
        355                 360                 365             


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
    370                 375                 380                 


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
385                 390                 395                 400 


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
                405                 410                 415     


Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr 
            420                 425                 430         


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
        435                 440                 445             


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
    450                 455                 460                 


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
465                 470                 475                 480 


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
                485                 490                 495     


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
            500                 505                 510         


Lys 
    


<210>  19
<211>  488
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A/Anhui/1/2005(H5N1) HA1(+28-259)-Fd-hFc fusion protein

<400>  19

His Ala Gln Asp Ile Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp 
1               5                   10                  15      


Leu Asp Gly Val Lys Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly 
            20                  25                  30          


Trp Leu Leu Gly Asn Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu 
        35                  40                  45              


Trp Ser Tyr Ile Val Glu Lys Ala Asn Pro Ala Asn Asp Leu Cys Tyr 
    50                  55                  60                  


Pro Gly Asn Phe Asn Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg 
65                  70                  75                  80  


Ile Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser 
                85                  90                  95      


Asp His Glu Ala Ser Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly 
            100                 105                 110         


Thr Pro Ser Phe Phe Arg Asn Val Val Trp Leu Ile Lys Lys Asn Asn 
        115                 120                 125             


Thr Tyr Pro Thr Ile Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp 
    130                 135                 140                 


Leu Leu Ile Leu Trp Gly Ile His His Ser Asn Asp Ala Ala Glu Gln 
145                 150                 155                 160 


Thr Lys Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser 
                165                 170                 175     


Thr Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val 
            180                 185                 190         


Asn Gly Gln Asn Gly Arg Met Asp Phe Phe Trp Thr Ile Leu Lys Pro 
        195                 200                 205             


Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu 
    210                 215                 220                 


Tyr Ala Tyr Lys Ile Val Lys Lys Gly Tyr Ile Pro Glu Ala Pro Arg 
225                 230                 235                 240 


Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser 
                245                 250                 255     


Thr Phe Leu Arg Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
            260                 265                 270         


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
        275                 280                 285             


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
    290                 295                 300                 


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
305                 310                 315                 320 


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
                325                 330                 335     


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
            340                 345                 350         


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
        355                 360                 365             


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
    370                 375                 380                 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
385                 390                 395                 400 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
                405                 410                 415     


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
            420                 425                 430         


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
        435                 440                 445             


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
    450                 455                 460                 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
465                 470                 475                 480 


Ser Leu Ser Leu Ser Pro Gly Lys 
                485             


<210>  20
<211>  471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A/Anhui/1/2005(H5N1) HA1(+45-259)-Fd-hFc fusion protein

<400>  20

Asp Gly Val Lys Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp 
1               5                   10                  15      


Leu Leu Gly Asn Pro Met Cys Asp Glu Phe Ile Asn Val Pro Glu Trp 
            20                  25                  30          


Ser Tyr Ile Val Glu Lys Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro 
        35                  40                  45              


Gly Asn Phe Asn Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile 
    50                  55                  60                  


Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp 
65                  70                  75                  80  


His Glu Ala Ser Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Thr 
                85                  90                  95      


Pro Ser Phe Phe Arg Asn Val Val Trp Leu Ile Lys Lys Asn Asn Thr 
            100                 105                 110         


Tyr Pro Thr Ile Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu 
        115                 120                 125             


Leu Ile Leu Trp Gly Ile His His Ser Asn Asp Ala Ala Glu Gln Thr 
    130                 135                 140                 


Lys Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser Val Gly Thr Ser Thr 
145                 150                 155                 160 


Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn 
                165                 170                 175     


Gly Gln Asn Gly Arg Met Asp Phe Phe Trp Thr Ile Leu Lys Pro Asn 
            180                 185                 190         


Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr 
        195                 200                 205             


Ala Tyr Lys Ile Val Lys Lys Gly Tyr Ile Pro Glu Ala Pro Arg Asp 
    210                 215                 220                 


Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr 
225                 230                 235                 240 


Phe Leu Arg Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
                245                 250                 255     


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            260                 265                 270         


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
        275                 280                 285             


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
    290                 295                 300                 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
305                 310                 315                 320 


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                325                 330                 335     


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
            340                 345                 350         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        355                 360                 365             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 
    370                 375                 380                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
385                 390                 395                 400 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                405                 410                 415     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            420                 425                 430         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        435                 440                 445             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    450                 455                 460                 


Leu Ser Leu Ser Pro Gly Lys 
465                 470     


<210>  21
<211>  444
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A/Anhui/1/2005(H5N1) HA1(+72-259)-Fd-hFc fusion protein

<400>  21

Asn Val Pro Glu Trp Ser Tyr Ile Val Glu Lys Ala Asn Pro Ala Asn 
1               5                   10                  15      


Asp Leu Cys Tyr Pro Gly Asn Phe Asn Asp Tyr Glu Glu Leu Lys His 
            20                  25                  30          


Leu Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys 
        35                  40                  45              


Ser Ser Trp Ser Asp His Glu Ala Ser Ser Gly Val Ser Ser Ala Cys 
    50                  55                  60                  


Pro Tyr Gln Gly Thr Pro Ser Phe Phe Arg Asn Val Val Trp Leu Ile 
65                  70                  75                  80  


Lys Lys Asn Asn Thr Tyr Pro Thr Ile Lys Arg Ser Tyr Asn Asn Thr 
                85                  90                  95      


Asn Gln Glu Asp Leu Leu Ile Leu Trp Gly Ile His His Ser Asn Asp 
            100                 105                 110         


Ala Ala Glu Gln Thr Lys Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser 
        115                 120                 125             


Val Gly Thr Ser Thr Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr 
    130                 135                 140                 


Arg Ser Lys Val Asn Gly Gln Asn Gly Arg Met Asp Phe Phe Trp Thr 
145                 150                 155                 160 


Ile Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe 
                165                 170                 175     


Ile Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys Lys Gly Tyr Ile Pro 
            180                 185                 190         


Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp 
        195                 200                 205             


Val Leu Leu Ser Thr Phe Leu Arg Ser Asp Lys Thr His Thr Cys Pro 
    210                 215                 220                 


Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
225                 230                 235                 240 


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
                245                 250                 255     


Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
            260                 265                 270         


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
        275                 280                 285             


Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
    290                 295                 300                 


Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
305                 310                 315                 320 


Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
                325                 330                 335     


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
            340                 345                 350         


Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
        355                 360                 365             


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
    370                 375                 380                 


Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
385                 390                 395                 400 


Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
                405                 410                 415     


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
            420                 425                 430         


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 


<210>  22
<211>  300
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A/Anhui/1/2005(H5N1) HA1(+72-115)-Fd-hFc fusion protein

<400>  22

Asn Val Pro Glu Trp Ser Tyr Ile Val Glu Lys Ala Asn Pro Ala Asn 
1               5                   10                  15      


Asp Leu Cys Tyr Pro Gly Asn Phe Asn Asp Tyr Glu Glu Leu Lys His 
            20                  25                  30          


Leu Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln Gly Tyr Ile Pro 
        35                  40                  45              


Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp 
    50                  55                  60                  


Val Leu Leu Ser Thr Phe Leu Arg Ser Asp Lys Thr His Thr Cys Pro 
65                  70                  75                  80  


Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
                85                  90                  95      


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
            100                 105                 110         


Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
        115                 120                 125             


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
    130                 135                 140                 


Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
145                 150                 155                 160 


Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
                165                 170                 175     


Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
            180                 185                 190         


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
        195                 200                 205             


Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
    210                 215                 220                 


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
225                 230                 235                 240 


Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
                245                 250                 255     


Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
            260                 265                 270         


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
        275                 280                 285             


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    290                 295                 300 


