                         SEQUENCE LISTING

<110>  Intervet
 
<120>  Herpesvirus of turkeys vectored vaccine against avian influenza 
       in poultry

<130>  2010-025-EP-PD

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  723
<212>  DNA
<213>  Equine herpesvirus 1

<400>  1
ggcgactgcg gatgcttcgc agcgcaggcg catgtacgcg gagcgtctgt caaagcgttc       60

catcgccagt ttggggcgct gcgtgcgcga acagcgaaga gaactagaaa aaaccctgag      120

agttaacgtg tatggcgaag tgctgctaca tacgtacgta tcgtcctaca acgggttttg      180

cgccaggcgc gggttttgcg cggcggtgag tcgagcgggt accatcatag ataaccgctc      240

tagcacgtcc gcgttcgact cgcatcagtt catgaaggcg gcgctgcttc gccaccccat      300

tgaccagtcg ctcatgccgt ccataacaca caagtttttc gagctgatca acgggcccgt      360

gtttgacaac gctggccaca actttgcgca gccgccaaac acggcattat attacagcgt      420

tgaaaacgtt gggttgttac cgcatctcaa ggaggaacta gctcggttta tgattactgc      480

ggctaaaggt gattggtcaa ttagcgagtt tcaaaggttt tattgctttg agggagtgac      540

aggtgtgacg gccacgcagc ggctggcgtg gaaatatatc ggggagctca tcctagccgc      600

cgcagtattc tcctcggttt tccactgtgg agaggtgcgc ctcctgcgcg cagatcgtac      660

ctacccggac tccagcggcg cacagcgctg cgtgagcggc atttacataa cctacgaggc      720

gtc                                                                    723


<210>  2
<211>  674
<212>  DNA
<213>  Pseudorabies virus

<400>  2
cgctgctgca cacgtacgtg gcggtggccg ccgggttccg cgcacggcgc gcgttctgcg       60

aggccgccgc gcgcgcgggc accgtcgtgg acgagcgcga gacgggctgc ttcgacgcgc      120

acagcttcat gaaggccacg gtgcagcgcc accccgtgga cgccgcgctc ctcccggcgc      180

tcacgcacaa gttcttcgag ctcgtcaacg ggccgctctt cgcgcacgac acgcacgcct      240

tcgcccagtc ccccaacacg gcgctctact ttgcggtgga gaacgtgggc ctcctgccgc      300

acctgaagga ggagctggcg cgcttcatgg tggcccgcga ttggtgcgtc agtgagttcc      360

gcggcttcta ccgcttccag acggccggcg taaccgccac ccagcggcag gcctggcgat      420

atatccgcga gctggtgctg gcggttgcag tcttcaggtc cgtcttccac tgcggggacg      480

tcgaggtcct ccgcgcggat cgcttcgccg gacgcgacgg gctgtacctg acctacgagg      540

cgtcttgccc gctggtggcg gtctttggcg cgggccccgc gggcatcggc ccgggcacca      600

cggcggtgct ggcctcggac gtctttggcc tgctccacac cacgctgctg ctgcgcgggg      660

cgccgtcgcg ctag                                                        674


<210>  3
<211>  1707
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimised HP AIV H5 HA gene


<220>
<221>  CDS
<222>  (1)..(1704)

<400>  3
atg gag aag atc gtc ctc ctg ctg gct atc gtc tcc ctg gtc aag agc         48
Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Ser           
1               5                   10                  15                

gac cag atc tgc atc ggc tac cac gcc aac aac tct acc gag cag gtg         96
Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val           
            20                  25                  30                    

gac acc atc atg gag aag aac gtg acc gtc act cac gcc cag gac atc        144
Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile           
        35                  40                  45                        

ctc gag aag act cac aac gga aag ctc tgc gac ctc gac ggc gtc aag        192
Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys           
    50                  55                  60                            

cct ctg atc ctg cgt gac tgc tcc gtg gct ggt tgg ctc ctg ggc aac        240
Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn           
65                  70                  75                  80            

ccc atg tgc gac gag ttc ctc aac gtg ccc gag tgg tcc tac atc gtc        288
Pro Met Cys Asp Glu Phe Leu Asn Val Pro Glu Trp Ser Tyr Ile Val           
                85                  90                  95                

gag aag atc aac ccc gcc aac gac ctg tgc tac cct ggc aac ttc aac        336
Glu Lys Ile Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn           
            100                 105                 110                   

gac tac gag gag ctc aag cac ctg ctc tcc cgt atc aac cac ttc gag        384
Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu           
        115                 120                 125                       

aag atc cag atc atc ccc aag tcc tcc tgg tcc gac cac gag gct tct        432
Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser           
    130                 135                 140                           

agc ggt gtg tcc agc gct tgc ccc tac cag ggc cgc tcc agc ttc ttc        480
Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Arg Ser Ser Phe Phe           
145                 150                 155                 160           

cgc aac gtc gtg tgg ctg atc aag aag gac aac gct tac cca act atc        528
Arg Asn Val Val Trp Leu Ile Lys Lys Asp Asn Ala Tyr Pro Thr Ile           
                165                 170                 175               

aag cgc agc tac aac aac act aac cag gag gac ctg ctg gtg ctg tgg        576
Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp           
            180                 185                 190                   

ggc atc cac cac cct aac gac gcc gct gag cag act cgt ctc tac cag        624
Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Arg Leu Tyr Gln           
        195                 200                 205                       

aac cct act agc tac atc tcc gtg gga acc tct acc ctg aac cag agg        672
Asn Pro Thr Ser Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg           
    210                 215                 220                           

ctg gtg ccc aag atc gct acc agg tcc aag gtc aac ggt cag tct ggt        720
Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly           
225                 230                 235                 240           

agg atg gag ttc ttc tgg act atc ctg aag ccc aac gac gct atc aac        768
Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn           
                245                 250                 255               

ttc gag tct aac ggt aac ttc atc gct cct gag aac gcc tac aag atc        816
Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Asn Ala Tyr Lys Ile           
            260                 265                 270                   

gtc aag aag ggt gac tct act atc atg aag tct gag ctg gag tac ggt        864
Val Lys Lys Gly Asp Ser Thr Ile Met Lys Ser Glu Leu Glu Tyr Gly           
        275                 280                 285                       

aac tgc aac acc aag tgc cag acc cct atc ggt gcc atc aac tcc tct        912
Asn Cys Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser           
    290                 295                 300                           

atg cct ttc cac aac atc cac ccc ctg acc atc ggt gag tgc cct aag        960
Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys           
305                 310                 315                 320           

tac gtc aag tct aac cgt ctg gtc ctg gct act gga ctg cgt aac tct       1008
Tyr Val Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser           
                325                 330                 335               

ccc cag ggt gag cgc cgt cgt aag aag agg ggc ctc ttc ggt gcc atc       1056
Pro Gln Gly Glu Arg Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile           
            340                 345                 350                   

gct ggc ttc atc gag ggt gga tgg cag ggc atg gtg gac ggc tgg tac       1104
Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr           
        355                 360                 365                       

ggt tac cac cac agc aac gag cag ggc tcc ggt tac gct gcc gac aag       1152
Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys           
    370                 375                 380                           

gag tct acc cag aag gct atc gac ggc gtc acc aac aag gtg aac tcc       1200
Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser           
385                 390                 395                 400           

atc atc gac aag atg aac acc cag ttc gag gct gtg ggc agg gag ttc       1248
Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe           
                405                 410                 415               

aac aac ctg gag cgt cgt atc gag aac ctg aac aag aag atg gag gac       1296
Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp           
            420                 425                 430                   

ggt ttc ctg gac gtc tgg act tac aac gcc gag ctc ctg gtg ctg atg       1344
Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met           
        435                 440                 445                       

gag aac gag cgc acc ctg gac ttc cac gac tcc aac gtg aag aac ctc       1392
Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu           
    450                 455                 460                           

tac gac aag gtc cgc ctc cag ctc cgc gac aac gct aag gag ctg ggt       1440
Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly           
465                 470                 475                 480           

aac ggt tgc ttc gag ttc tac cac agg tgc gac aac gag tgc atg gag       1488
Asn Gly Cys Phe Glu Phe Tyr His Arg Cys Asp Asn Glu Cys Met Glu           
                485                 490                 495               

tcc gtg cgt aac ggc acc tac gac tac ccc cag tac tcc gag gag gcc       1536
Ser Val Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala           
            500                 505                 510                   

cgt ctc aag agg gag gag atc tcc ggt gtg cgc ctg gag agc atc ggt       1584
Arg Leu Lys Arg Glu Glu Ile Ser Gly Val Arg Leu Glu Ser Ile Gly           
        515                 520                 525                       

act tac cag atc ctc tcc atc tac tcc acc gtc gcc agc tcc ctc gcc       1632
Thr Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala           
    530                 535                 540                           

ctg gct atc atg gtg gct ggc ctc tcc ctg tgg atg tgc tcc aac ggc       1680
Leu Ala Ile Met Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly           
545                 550                 555                 560           

agc ctg cag tgc aag atc tgc atc taa                                   1707
Ser Leu Gln Cys Lys Ile Cys Ile                                           
                565                                                       


<210>  4
<211>  568
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4

Met Glu Lys Ile Val Leu Leu Leu Ala Ile Val Ser Leu Val Lys Ser 
1               5                   10                  15      


Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Gln Val 
            20                  25                  30          


Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala Gln Asp Ile 
        35                  40                  45              


Leu Glu Lys Thr His Asn Gly Lys Leu Cys Asp Leu Asp Gly Val Lys 
    50                  55                  60                  


Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu Leu Gly Asn 
65                  70                  75                  80  


Pro Met Cys Asp Glu Phe Leu Asn Val Pro Glu Trp Ser Tyr Ile Val 
                85                  90                  95      


Glu Lys Ile Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asn Phe Asn 
            100                 105                 110         


Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Arg Ile Asn His Phe Glu 
        115                 120                 125             


Lys Ile Gln Ile Ile Pro Lys Ser Ser Trp Ser Asp His Glu Ala Ser 
    130                 135                 140                 


Ser Gly Val Ser Ser Ala Cys Pro Tyr Gln Gly Arg Ser Ser Phe Phe 
145                 150                 155                 160 


Arg Asn Val Val Trp Leu Ile Lys Lys Asp Asn Ala Tyr Pro Thr Ile 
                165                 170                 175     


Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu Val Leu Trp 
            180                 185                 190         


Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Arg Leu Tyr Gln 
        195                 200                 205             


Asn Pro Thr Ser Tyr Ile Ser Val Gly Thr Ser Thr Leu Asn Gln Arg 
    210                 215                 220                 


Leu Val Pro Lys Ile Ala Thr Arg Ser Lys Val Asn Gly Gln Ser Gly 
225                 230                 235                 240 


Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp Ala Ile Asn 
                245                 250                 255     


Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Asn Ala Tyr Lys Ile 
            260                 265                 270         


Val Lys Lys Gly Asp Ser Thr Ile Met Lys Ser Glu Leu Glu Tyr Gly 
        275                 280                 285             


Asn Cys Asn Thr Lys Cys Gln Thr Pro Ile Gly Ala Ile Asn Ser Ser 
    290                 295                 300                 


Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu Cys Pro Lys 
305                 310                 315                 320 


Tyr Val Lys Ser Asn Arg Leu Val Leu Ala Thr Gly Leu Arg Asn Ser 
                325                 330                 335     


Pro Gln Gly Glu Arg Arg Arg Lys Lys Arg Gly Leu Phe Gly Ala Ile 
            340                 345                 350         


Ala Gly Phe Ile Glu Gly Gly Trp Gln Gly Met Val Asp Gly Trp Tyr 
        355                 360                 365             


Gly Tyr His His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys 
    370                 375                 380                 


Glu Ser Thr Gln Lys Ala Ile Asp Gly Val Thr Asn Lys Val Asn Ser 
385                 390                 395                 400 


Ile Ile Asp Lys Met Asn Thr Gln Phe Glu Ala Val Gly Arg Glu Phe 
                405                 410                 415     


Asn Asn Leu Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp 
            420                 425                 430         


Gly Phe Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met 
        435                 440                 445             


Glu Asn Glu Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu 
    450                 455                 460                 


Tyr Asp Lys Val Arg Leu Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly 
465                 470                 475                 480 


Asn Gly Cys Phe Glu Phe Tyr His Arg Cys Asp Asn Glu Cys Met Glu 
                485                 490                 495     


Ser Val Arg Asn Gly Thr Tyr Asp Tyr Pro Gln Tyr Ser Glu Glu Ala 
            500                 505                 510         


Arg Leu Lys Arg Glu Glu Ile Ser Gly Val Arg Leu Glu Ser Ile Gly 
        515                 520                 525             


Thr Tyr Gln Ile Leu Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Ala 
    530                 535                 540                 


Leu Ala Ile Met Val Ala Gly Leu Ser Leu Trp Met Cys Ser Asn Gly 
545                 550                 555                 560 


Ser Leu Gln Cys Lys Ile Cys Ile 
                565             


<210>  5
<211>  1683
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimised HP AIV H7 HA gene


<220>
<221>  CDS
<222>  (1)..(1680)

<400>  5
atg aac act cag atc ctg gta ttc gct ctg gtg gcg atc atc cca acc         48
Met Asn Thr Gln Ile Leu Val Phe Ala Leu Val Ala Ile Ile Pro Thr           
1               5                   10                  15                

aac gcc gac aag atc tgc ctg gga cac cac gcc gtc tcc aac gga act         96
Asn Ala Asp Lys Ile Cys Leu Gly His His Ala Val Ser Asn Gly Thr           
            20                  25                  30                    

aag gtc aac acc ttg act gag cgt ggc gtg gag gtg gtc aac gct act        144
Lys Val Asn Thr Leu Thr Glu Arg Gly Val Glu Val Val Asn Ala Thr           
        35                  40                  45                        

gag acc gtg gag cgc act aac gtc ccc cgt atc tgc tcc aaa ggt aag        192
Glu Thr Val Glu Arg Thr Asn Val Pro Arg Ile Cys Ser Lys Gly Lys           
    50                  55                  60                            

cgt acc gtg gac ctc ggt cag tgc ggc ctg ctg ggt act atc act ggc        240
Arg Thr Val Asp Leu Gly Gln Cys Gly Leu Leu Gly Thr Ile Thr Gly           
65                  70                  75                  80            

ccc ccc cag tgc gac cag ttc ctg gag ttc tct gcc gac ctg atc atc        288
Pro Pro Gln Cys Asp Gln Phe Leu Glu Phe Ser Ala Asp Leu Ile Ile           
                85                  90                  95                

gag cgt cgc gag ggt tcc gac gtc tgc tac cct ggc aag ttc gtg aac        336
Glu Arg Arg Glu Gly Ser Asp Val Cys Tyr Pro Gly Lys Phe Val Asn           
            100                 105                 110                   

gag gag gct ctg cgt cag atc ctc cgc gag tcc ggc ggt atc gac aag        384
Glu Glu Ala Leu Arg Gln Ile Leu Arg Glu Ser Gly Gly Ile Asp Lys           
        115                 120                 125                       

gag acc atg ggc ttc acc tac agc ggt atc cgc act aac ggc gcc acc        432
Glu Thr Met Gly Phe Thr Tyr Ser Gly Ile Arg Thr Asn Gly Ala Thr           
    130                 135                 140                           

tcc gct tgc cgc cgt tcc ggt tct tcc ttc tac gcc gag atg aag tgg        480
Ser Ala Cys Arg Arg Ser Gly Ser Ser Phe Tyr Ala Glu Met Lys Trp           
145                 150                 155                 160           

ctc ctg tcc agc act gac aac gct gct ttc ccc cag atg act aag tcc        528
Leu Leu Ser Ser Thr Asp Asn Ala Ala Phe Pro Gln Met Thr Lys Ser           
                165                 170                 175               

tac aag aac acc cgc aag gac cct gct ctg atc atc tgg ggc atc cac        576
Tyr Lys Asn Thr Arg Lys Asp Pro Ala Leu Ile Ile Trp Gly Ile His           
            180                 185                 190                   

cac tcc ggt tcc acc act gag cag acc aag ctg tac ggc tcc ggt aac        624
His Ser Gly Ser Thr Thr Glu Gln Thr Lys Leu Tyr Gly Ser Gly Asn           
        195                 200                 205                       

aag ctc atc acc gtc ggc tct tct aac tac cag cag tcc ttc atc ccc        672
Lys Leu Ile Thr Val Gly Ser Ser Asn Tyr Gln Gln Ser Phe Ile Pro           
    210                 215                 220                           

tct ccc ggt gcc cgc cct cag gtg aac ggc cag tct ggc cgc atc gac        720
Ser Pro Gly Ala Arg Pro Gln Val Asn Gly Gln Ser Gly Arg Ile Asp           
225                 230                 235                 240           

ttc cac tgg ctg atc ctg aac ccc aac gac act atc act ttc tcc ttc        768
Phe His Trp Leu Ile Leu Asn Pro Asn Asp Thr Ile Thr Phe Ser Phe           
                245                 250                 255               

aac ggt gcc ttc atc gct cct gac cgt gct agc ttc ctg cgt ggc aag        816
Asn Gly Ala Phe Ile Ala Pro Asp Arg Ala Ser Phe Leu Arg Gly Lys           
            260                 265                 270                   

tct atg ggt atc cag tcc ggt gtc cag gtg gac gcc aac tgc gag ggt        864
Ser Met Gly Ile Gln Ser Gly Val Gln Val Asp Ala Asn Cys Glu Gly           
        275                 280                 285                       

gac tgc tac cac tct ggc ggt acc atc atc agc aac ctg ccc ttc cag        912
Asp Cys Tyr His Ser Gly Gly Thr Ile Ile Ser Asn Leu Pro Phe Gln           
    290                 295                 300                           

aac atc aac agc cgc gcc gtc ggc aag tgc cct cgc tac gtc aag cag        960
Asn Ile Asn Ser Arg Ala Val Gly Lys Cys Pro Arg Tyr Val Lys Gln           
305                 310                 315                 320           

gag tcc ctg atg ctg gct act ggt atg aag aac gtg ccc gag atc cct       1008
Glu Ser Leu Met Leu Ala Thr Gly Met Lys Asn Val Pro Glu Ile Pro           
                325                 330                 335               

aag ggc cgt ggc ctg ttc ggc gct atc gcc ggt ttc atc gag aac ggt       1056
Lys Gly Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly           
            340                 345                 350                   

tgg gag ggt ctg atc gac ggc tgg tac ggc ttc agg cac cag aac gcc       1104
Trp Glu Gly Leu Ile Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ala           
        355                 360                 365                       

cag ggt gag ggc act gct gct gac tac aag agc acc cag tcc gcc atc       1152
Gln Gly Glu Gly Thr Ala Ala Asp Tyr Lys Ser Thr Gln Ser Ala Ile           
    370                 375                 380                           

gac cag atc acc ggt aag ctg aac cgt ctc atc gag aag act aac cag       1200
Asp Gln Ile Thr Gly Lys Leu Asn Arg Leu Ile Glu Lys Thr Asn Gln           
385                 390                 395                 400           

cag ttc gag ctc atc gac aac gag ttc act gag gtc gag aag cag atc       1248
Gln Phe Glu Leu Ile Asp Asn Glu Phe Thr Glu Val Glu Lys Gln Ile           
                405                 410                 415               

ggc aac gtg atc aac tgg acc agg gac tcc atg act gag gtg tgg tcc       1296
Gly Asn Val Ile Asn Trp Thr Arg Asp Ser Met Thr Glu Val Trp Ser           
            420                 425                 430                   

tac aac gct gag ctc ctc gtc gcc atg gag aac cag cac acc atc gac       1344
Tyr Asn Ala Glu Leu Leu Val Ala Met Glu Asn Gln His Thr Ile Asp           
        435                 440                 445                       

ctg gct gac tcc gag atg aac aag ctc tac gag cgt gtg agg agg cag       1392
Leu Ala Asp Ser Glu Met Asn Lys Leu Tyr Glu Arg Val Arg Arg Gln           
    450                 455                 460                           

ctg cgc gag aac gct gag gag gac ggt act ggt tgc ttc gag atc ttc       1440
Leu Arg Glu Asn Ala Glu Glu Asp Gly Thr Gly Cys Phe Glu Ile Phe           
465                 470                 475                 480           

cac aag tgc gac gac gac tgc atg gcc tcc atc cgt aac aac acc tac       1488
His Lys Cys Asp Asp Asp Cys Met Ala Ser Ile Arg Asn Asn Thr Tyr           
                485                 490                 495               

gac cac agc aag tac agg gag gag gcc atg cag aac agg atc cag atc       1536
Asp His Ser Lys Tyr Arg Glu Glu Ala Met Gln Asn Arg Ile Gln Ile           
            500                 505                 510                   

gac ccc gtc aag ctg agc agc ggc tac aag gac gtg atc ctg tgg ttc       1584
Asp Pro Val Lys Leu Ser Ser Gly Tyr Lys Asp Val Ile Leu Trp Phe           
        515                 520                 525                       

agc ttc ggc gct tcc tgc ttc atc ctc ctg gcc atc gcc atg ggc ctg       1632
Ser Phe Gly Ala Ser Cys Phe Ile Leu Leu Ala Ile Ala Met Gly Leu           
    530                 535                 540                           

gtc ttc atc tgc gtg aag aac ggt aac atg agg tgc act atc tgc atc       1680
Val Phe Ile Cys Val Lys Asn Gly Asn Met Arg Cys Thr Ile Cys Ile           
545                 550                 555                 560           

taa                                                                   1683


<210>  6
<211>  560
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  6

Met Asn Thr Gln Ile Leu Val Phe Ala Leu Val Ala Ile Ile Pro Thr 
1               5                   10                  15      


Asn Ala Asp Lys Ile Cys Leu Gly His His Ala Val Ser Asn Gly Thr 
            20                  25                  30          


Lys Val Asn Thr Leu Thr Glu Arg Gly Val Glu Val Val Asn Ala Thr 
        35                  40                  45              


Glu Thr Val Glu Arg Thr Asn Val Pro Arg Ile Cys Ser Lys Gly Lys 
    50                  55                  60                  


Arg Thr Val Asp Leu Gly Gln Cys Gly Leu Leu Gly Thr Ile Thr Gly 
65                  70                  75                  80  


Pro Pro Gln Cys Asp Gln Phe Leu Glu Phe Ser Ala Asp Leu Ile Ile 
                85                  90                  95      


Glu Arg Arg Glu Gly Ser Asp Val Cys Tyr Pro Gly Lys Phe Val Asn 
            100                 105                 110         


Glu Glu Ala Leu Arg Gln Ile Leu Arg Glu Ser Gly Gly Ile Asp Lys 
        115                 120                 125             


Glu Thr Met Gly Phe Thr Tyr Ser Gly Ile Arg Thr Asn Gly Ala Thr 
    130                 135                 140                 


Ser Ala Cys Arg Arg Ser Gly Ser Ser Phe Tyr Ala Glu Met Lys Trp 
145                 150                 155                 160 


Leu Leu Ser Ser Thr Asp Asn Ala Ala Phe Pro Gln Met Thr Lys Ser 
                165                 170                 175     


Tyr Lys Asn Thr Arg Lys Asp Pro Ala Leu Ile Ile Trp Gly Ile His 
            180                 185                 190         


His Ser Gly Ser Thr Thr Glu Gln Thr Lys Leu Tyr Gly Ser Gly Asn 
        195                 200                 205             


Lys Leu Ile Thr Val Gly Ser Ser Asn Tyr Gln Gln Ser Phe Ile Pro 
    210                 215                 220                 


Ser Pro Gly Ala Arg Pro Gln Val Asn Gly Gln Ser Gly Arg Ile Asp 
225                 230                 235                 240 


Phe His Trp Leu Ile Leu Asn Pro Asn Asp Thr Ile Thr Phe Ser Phe 
                245                 250                 255     


Asn Gly Ala Phe Ile Ala Pro Asp Arg Ala Ser Phe Leu Arg Gly Lys 
            260                 265                 270         


Ser Met Gly Ile Gln Ser Gly Val Gln Val Asp Ala Asn Cys Glu Gly 
        275                 280                 285             


Asp Cys Tyr His Ser Gly Gly Thr Ile Ile Ser Asn Leu Pro Phe Gln 
    290                 295                 300                 


Asn Ile Asn Ser Arg Ala Val Gly Lys Cys Pro Arg Tyr Val Lys Gln 
305                 310                 315                 320 


Glu Ser Leu Met Leu Ala Thr Gly Met Lys Asn Val Pro Glu Ile Pro 
                325                 330                 335     


Lys Gly Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly 
            340                 345                 350         


Trp Glu Gly Leu Ile Asp Gly Trp Tyr Gly Phe Arg His Gln Asn Ala 
        355                 360                 365             


Gln Gly Glu Gly Thr Ala Ala Asp Tyr Lys Ser Thr Gln Ser Ala Ile 
    370                 375                 380                 


Asp Gln Ile Thr Gly Lys Leu Asn Arg Leu Ile Glu Lys Thr Asn Gln 
385                 390                 395                 400 


Gln Phe Glu Leu Ile Asp Asn Glu Phe Thr Glu Val Glu Lys Gln Ile 
                405                 410                 415     


Gly Asn Val Ile Asn Trp Thr Arg Asp Ser Met Thr Glu Val Trp Ser 
            420                 425                 430         


Tyr Asn Ala Glu Leu Leu Val Ala Met Glu Asn Gln His Thr Ile Asp 
        435                 440                 445             


Leu Ala Asp Ser Glu Met Asn Lys Leu Tyr Glu Arg Val Arg Arg Gln 
    450                 455                 460                 


Leu Arg Glu Asn Ala Glu Glu Asp Gly Thr Gly Cys Phe Glu Ile Phe 
465                 470                 475                 480 


His Lys Cys Asp Asp Asp Cys Met Ala Ser Ile Arg Asn Asn Thr Tyr 
                485                 490                 495     


Asp His Ser Lys Tyr Arg Glu Glu Ala Met Gln Asn Arg Ile Gln Ile 
            500                 505                 510         


Asp Pro Val Lys Leu Ser Ser Gly Tyr Lys Asp Val Ile Leu Trp Phe 
        515                 520                 525             


Ser Phe Gly Ala Ser Cys Phe Ile Leu Leu Ala Ile Ala Met Gly Leu 
    530                 535                 540                 


Val Phe Ile Cys Val Lys Asn Gly Asn Met Arg Cys Thr Ile Cys Ile 
545                 550                 555                 560 


<210>  7
<211>  9792
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EcoR-EcoR1 insert HVP310


<220>
<221>  misc_feature
<222>  (1)..(3661)
<223>  HVT Upstream Us2 area

<220>
<221>  misc_feature
<222>  (3683)..(4357)
<223>  PRV gB promoter, extended

<220>
<221>  misc_feature
<222>  (4361)..(6096)
<223>  Codon optimised H5 HA gene

<220>
<221>  misc_feature
<222>  (6139)..(9787)
<223>  HVT Us2 downstream area

<400>  7
gaattccaga ctaaatgccc cggcccaatt tgtcaagtgt gcagtcacgg aggcgtcgac       60

cgtgtccccg gcattaaaca ggaaagcgtt aaagtttttg aatgttaggt cacaggtaca      120

aacataaatg tttgtacaaa caggtaacag gtacaaacat aaatgccccg gcataaatgt      180

cccttacggc ggatcgaaac gacattaggc atactcgggt accattttgc attccgatca      240

gcacggatga aattaggcag gaatgcggtt tatattatgc ggcattggac aaacgatatg      300

gcattgattg gcagtttatg aatgtcttca tgttgggcgt aaacggattc ctattggttc      360

agaagacaac gacgatatat ttagagagaa aaagctaccc agcataggat aaacacacat      420

tgagcattga gagacatagg tatcggtatg gatgggaaaa ctacacacgt gaacaccaaa      480

cgacttatat actcgagcgg tgatactact gagcaagaat gcactgcatc tgagccactg      540

aatgaagact gtgatgaaaa tgtgaccatc gatggaattg gagaagaata tgcgcagttc      600

ttcatgtccc cgcaatgggt cccaaatcta catcgcttga gcgaggatac caaaaaggta      660

taccgatgta tggtttccaa cagactcaat tattttccct attatgaggc gttcaggcgg      720

tctttgtttg atatgtatat gctaggtcgg ttggggcgtc gacttaagcg atctgactgg      780

gagactatta tgcatctgtc accaacgcaa agtcggcgtc tacatagaac tttaagattt      840

gtggagcgta gaattatccc atctaacagt tatatacgca catcgggcca cgttccgcct      900

tcgagggcac ttccgacaga tacgaattta aagatggatg aataattaaa ttggaaagag      960

taactacatt aatcgagcgt catgacggcg tcccgtgaaa atgggaattt tctactcgaa     1020

acaccgtgac atttgacaga cctggaattg ttattctgat atatagtggg tgtgtctggc     1080

cggcaacata cataatgtgc atgcgaaacc actttttcag tgtacgctga cattgtgcaa     1140

cacggagggg tagcatctac atacaatata tgttgattaa tgattggaga aaaaactatg     1200

cagctcgccg atcatatggc taactcgcct tcgtctatat ggcggacccc gcgggaaaaa     1260

tcgacgtacc atctgattta caacaccagt aatgaacatg tcgcatccct gcccagatct     1320

gtgcgcccat tggcgcggat cgttgtgaat gccgccgaaa cacttcaggt cggtatgaga     1380

gccgggaggc cgccatcagc aggagtttgg cgagaggtgt ttgatagaat gatgacagcc     1440

ttccgtgacc acgagcctac tgcgacattt aatgctgcaa atcccattag aaaaatggtc     1500

gagacagttc tacagaataa tgaagagccc ccgcggacgc atgctgaaat gggtaatcgc     1560

cttatgaaca ttatgtactg gtgttgcttg ggacacgcag gacaatgctc gatatggcag     1620

ttgtacgaga cgaatcaggc cattttaagt ttattagatg aagtggttat cggcacaaca     1680

aatccctttt gcaccctcga gcaatactgg aagccattat gcaccgcaat cgccaacaag     1740

gggacctcat cgcttgttga ggatgccaaa gtggccgagt acctggttag catgcgcaaa     1800

ttgatataac ataggcacgc tctgatgtta cagaccacaa taccgcatac atttattgta     1860

aggttgttaa taaaggttta ttctatgtaa gactacaata ctttcgacat tgcttgtata     1920

catattaaat actttctcaa gttcctatta cataaaatgg gatctatcat tacattcgtt     1980

aagagtctgg ataattttac tgtttgccag cttcgatctt ggaacgtact gtggatagtg     2040

ccttacttgg aatcgtgaaa atttgaaacg tccattattt ggatatcttc cggttgtccc     2100

atatcccgcc ctggtaccgc tcggatacct tgcccgtatg gattcgtatt gacagtcgcg     2160

caatcgggga ccaacaacgc gtgggtccac actcattcgg aaattttccg atgattctga     2220

atatttattg ccgctcgtta cgagtcgttg gacatatctg taatacattt cttcttctga     2280

aggatcgctg cacatttgat ctatacattg gccaggatgt tcaagtctca gatgttgcat     2340

tctggcacag cacaacttta tggcatttcc gatgtaatcg tccggcagcc ctgggggagt     2400

tctatattcg catattggga tggtaaggac aatagcagat ctcgcaacct ccagggaggc     2460

tataataacg tttttaaagg atggatttct cataaaaatc tgtcgcaaat tacactgaga     2520

atatccttta ctagcgccga ttgagagcat cgtcgtccaa ttttctaaat ggaaagaaaa     2580

caaggcgggc aagagtgttc caaacatttt cattttcggc gaatctctca aatcccatgg     2640

cgtgcaattg attgcaaaat tggcacttcc gttcacgttt gtatctccaa actctaagac     2700

acttttaatt gaaaaactac gttctagtgt ggaaagaaac ctataggcag accatagaac     2760

tatttgacac cacatatctt tttgtatgtc aaactgacca tgatcgtatg ttgctgaatg     2820

cactagggca attcgctcgc gcgactccat acattgaata attccacacg tcagctcatc     2880

ggttagcaag gtccagtagt tgaagtcatt tatttttccc cgcggctggc caaatctacc     2940

tctgggaata tccaagttgt cgaatatgat cgcaccggct ctggtcatgg tgaaggaact     3000

gtagcataaa gacgcaggta tcataggggt aatatttttt tattcactca catactaaaa     3060

gtaacgcata ttagcaccat gtatgggcta tcaattgaca tttgcgtagc actacatcac     3120

gattatgtac aacataatgg gacaacatat ggcaagtaga tgcaatttcc tcacactagt     3180

tgggtttatc tactattgaa ttttccccta tctgtgatac acttgggagc ctctacaagc     3240

atattgccat catgtacgtt tttatctact gtcttaacgc ccatgggaac ggaggcgtcg     3300

tcgtcatgta ttggacggca acataggcag caacacaaat tgcgtttagg tggggtgcat     3360

gtggactcga taccaagccc ctgcagctgg ggaacgtctg gtggagagcc gataatttga     3420

tatacgcacg ccatattact gtcgttgaag tacgccttat cttctatgtt ttcaaattta     3480

ggttcccaag tggacgtgag aagtgtttgt atctcacatg gaatggccca aggcattcca     3540

gcccaggtgc ctggtacttt aatggcaaac aaacgttttg gtagaggtat tgattctatt     3600

gcagttctgc agatatctgc agccccgagt atccacaggc tatacgatac gttatcggag     3660

gcaagcttgg cgcgccggat ctcgctgctg cacacgtacg tggcggtggc cgccgggttc     3720

cgcgcacggc gcgcgttctg cgaggccgcc gcgcgcgcgg gcaccgtcgt ggacgagcgc     3780

gagacgggct gcttcgacgc gcacagcttc atgaaggcca cggtgcagcg ccaccccgtg     3840

gacgccgcgc tcctcccggc gctcacgcac aagttcttcg agctcgtcaa cgggccgctc     3900

ttcgcgcacg acacgcacgc cttcgcccag tcccccaaca cggcgctcta ctttgcggtg     3960

gagaacgtgg gcctcctgcc gcacctgaag gaggagctgg cgcgcttcat ggtggcccgc     4020

gattggtgcg tcagtgagtt ccgcggcttc taccgcttcc agacggccgg cgtaaccgcc     4080

acccagcggc aggcctggcg atatatccgc gagctggtgc tggcggttgc agtcttcagg     4140

tccgtcttcc actgcgggga cgtcgaggtc ctccgcgcgg atcgcttcgc cggacgcgac     4200

gggctgtacc tgacctacga ggcgtcttgc cccgctggtg gcggtctttg gcgcgggccc     4260

cgcgggcatc ggcccgggca ccacggcggt gctggcctcg gacgtctttg gcctgctcca     4320

caccacgctg ctgctgcgcg gggcgccgtc gcgctagaga tccaagatat caaagccatg     4380

gagaagatcg tcctcctgct ggctatcgtc tccctggtca agagcgacca gatctgcatc     4440

ggctaccacg ccaacaactc taccgagcag gtggacacca tcatggagaa gaacgtgacc     4500

gtcactcacg cccaggacat cctcgagaag actcacaacg gaaagctctg cgacctcgac     4560

ggcgtcaagc ctctgatcct gcgtgactgc tccgtggctg gttggctcct gggcaacccc     4620

atgtgcgacg agttcctcaa cgtgcccgag tggtcctaca tcgtcgagaa gatcaacccc     4680

gccaacgacc tgtgctaccc tggcaacttc aacgactacg aggagctcaa gcacctgctc     4740

tcccgtatca accacttcga gaagatccag atcatcccca agtcctcctg gtccgaccac     4800

gaggcttcta gcggtgtgtc cagcgcttgc ccctaccagg gccgctccag cttcttccgc     4860

aacgtcgtgt ggctgatcaa gaaggacaac gcttacccaa ctatcaagcg cagctacaac     4920

aacactaacc aggaggacct gctggtgctg tggggcatcc accaccctaa cgacgccgct     4980

gagcagactc gtctctacca gaaccctact agctacatct ccgtgggaac ctctaccctg     5040

aaccagaggc tggtgcccaa gatcgctacc aggtccaagg tcaacggtca gtctggtagg     5100

atggagttct tctggactat cctgaagccc aacgacgcta tcaacttcga gtctaacggt     5160

aacttcatcg ctcctgagaa cgcctacaag atcgtcaaga agggtgactc tactatcatg     5220

aagtctgagc tggagtacgg taactgcaac accaagtgcc agacccctat cggtgccatc     5280

aactcctcta tgcctttcca caacatccac cccctgacca tcggtgagtg ccctaagtac     5340

gtcaagtcta accgtctggt cctggctact ggactgcgta actctcccca gggtgagcgc     5400

cgtcgtaaga agaggggcct cttcggtgcc atcgctggct tcatcgaggg tggatggcag     5460

ggcatggtgg acggctggta cggttaccac cacagcaacg agcagggctc cggttacgct     5520

gccgacaagg agtctaccca gaaggctatc gacggcgtca ccaacaaggt gaactccatc     5580

atcgacaaga tgaacaccca gttcgaggct gtgggcaggg agttcaacaa cctggagcgt     5640

cgtatcgaga acctgaacaa gaagatggag gacggtttcc tggacgtctg gacttacaac     5700

gccgagctcc tggtgctgat ggagaacgag cgcaccctgg acttccacga ctccaacgtg     5760

aagaacctct acgacaaggt ccgcctccag ctccgcgaca acgctaagga gctgggtaac     5820

ggttgcttcg agttctacca caggtgcgac aacgagtgca tggagtccgt gcgtaacggc     5880

acctacgact acccccagta ctccgaggag gcccgtctca agagggagga gatctccggt     5940

gtgcgcctgg agagcatcgg tacttaccag atcctctcca tctactccac cgtcgccagc     6000

tccctcgccc tggctatcat ggtggctggc ctctccctgt ggatgtgctc caacggcagc     6060

ctgcagtgca agatctgcat ctaactggat atcaaggatc tctcgaggat atcctgcagg     6120

tcgactctag gaagcttgcc tccgattcta gcattacata gccggtcagt agatcctgcc     6180

attcggtagc gcaaccggct acatcttcaa acagtctcac gataaatgca tctctcgttc     6240

ctgccaatcc ggaaccgggc ataccactcc cgcctgccga tttaattctc acaattgggc     6300

gatgccggcg gggcaaaacg aatgtggatt tggcaaaccg acacaggtct gctgtacgga     6360

ctaatatggg cacacccaca tcattcttca gatgctccat gcattgttct atgagaaaga     6420

tccatagggt ggaggcagcg tcacgagatc gcccaggcaa tcgatcgcat tcgtctagta     6480

aagtgacgag agttatcatg cacacaccca tgcccacgcc ttccgaataa ctggagctgt     6540

ggaagatcgg aaacgtcttt ttgactgccg gtctcgtact actttcgcac aggtgtatac     6600

ccggacgcgt actatatatt ttatatcatc caacgtccga aattacatac gtggcggcga     6660

tggaagtaga tgttgagtct tcgaaagtaa gtgcctcgaa tatgggtatt gtctgtgaaa     6720

atatcgaaag cggtacgacg gttgcagaac cgtcgatgtc gccagatact agtaacaata     6780

gcttcgataa cgaagacttc cgtgggcctg aatacgatgt ggagataaat accagaaaat     6840

ctgctaatct tgatcgtatg gaatcttcgt gccgtgaaca acgagcggcg tgcgaacttc     6900

gaaagtgttc gtgtcctacg tctgccgtgc gcatgcaata cagtattctt tcatctctcg     6960

ctccgggttc agagggtcat gtatatatat gtactagata cggggacgcg gaccaaaaaa     7020

aatgcatagt gaaggcagtc gttggaggaa agaatcccgg gagggaagtg gatattttaa     7080

aaaccatctc acataaatca attataaaat taatccatgc ctataaatgg aaaaatgttg     7140

tgtgtatggc aatgcgtgta tatcgttatg atcttttcac atatattgac ggagtcggcc     7200

ctatgcccct tcaacagatg atctatattc aacgtggact actagaggcg ctagcataca     7260

tacatgaaag gggcatcatt caccgagacg taaagacgga gaatatattc ttggataatc     7320

acgaaaatgc agttttgggt gacttcggtg ctgcatgcca actaggagat tgtatagata     7380

cgccccaatg ttacggttgg agcggaactg tggaaacaaa ttcgccggaa ttatctgcac     7440

ttgatccgta ttgcacaaaa acagatattt ggagtgccgg attggttcta tatgagatgg     7500

caattaaaaa tgtaccattg tttagtaagc aggtgaaaag ttcgggatct cagctgagat     7560

ccataatacg gtgcatgcaa gtgcatgaac tggagtttcc ccgcaacgat tctaccaacc     7620

tctgtaaaca tttcaaacaa tatgcggttc gtgtacgacc gccttatacc attcctcgag     7680

ttataagaaa tggggggatg ccaatggatg ttgaatatgt catttctaaa atgcttacgt     7740

ttgaccagga gttcagacct tctgctaagg aaatattgaa tatgccccta tttactaagg     7800

cgccgattaa cctgcttaat atcacaccct ctgacagtgt ctaacggtat acaggcggga     7860

gcgggtcgtg gcgtcatcat caccacttga gaatttatat tttgaattgt tgattgataa     7920

attaacctga ttcattgaga actgaaacgc catattggtt tcttggatat gtctacaaca     7980

attagttaaa ttgctatgtt ctactgcgag taacatttga taagttgtaa gagacgggcg     8040

actcatgtcg aagttgacga atataaagta cataacgtgt ttagaatacc cagaatccga     8100

atagtccgcg ggggcgtctt ctcgcgtgag taccaaatac tgagttgaac ttgaaaatgc     8160

taaatctgtg acactctttg tgtgatgatt attgtcacca cttcgaagat ggcttcgaca     8220

ttcatgatgt tctggtgttt gtttggaatc gtaatagcgc ttgtttcgtc caagtctgac     8280

aacaaagaaa atctgaagaa ttatatcacg gataagtcaa ccaatattag aatacccacg     8340

ccattatttg tatcaacgga aaactcttat cccacaaaac atgtaatcta cgatgaaaac     8400

tgtggcttcg ctgtactcaa tcctataagt gaccccaaat atgtcctttt gagccagctt     8460

ctaatgggaa ggcgcaaata tgatgcgacg gtcgcgtggt ttgttctcgg taaaatgtgt     8520

gccagattaa tatatttgcg cgaattttat aactgctcga caaatgagcc ttttggcaca     8580

tgttctatga gctctcctgg atggtgggac aggcgctacg tctcaaccag tttcatttct     8640

cgcgacgaat tacagctggt ttttgcagcg ccgtcccgag aattagatgg tttatatacg     8700

cgcgtagtag ttgtcaacgg ggactttact acggccgata taatgtttaa tgttaaagtg     8760

gcatgtgcct tttcaaagac tggaatagaa gatgatacat tatgcaaacc ctttcatttc     8820

tttgccaatg caacattgca caatttaacc atgattagat cggtaactct tcgagcgcac     8880

gaaagccatt taaaggaatg ggtggcacgg agaggtggta acgtccctgc agtgctactt     8940

gagtctacca tgtatcatgc atccaatctg cctagaaatt tcagggattt ctacataaag     9000

tctccagatg attataagta taatcaccta gatgggccat ctgtaatgct catcactgac     9060

agacctagtg aagatttgga tgggaggctc gttcaccaaa gtgacatttt tactactaca     9120

agtcctataa aacaggtccg gtatgaagag catcagtcac atacaaagca gtatcctgta     9180

aacaaaatac aagctataat ttttttgata gggttaggct cgttcattgg aagcatattc     9240

gtagttttgg tagtatggat tatacgcaga tattgcaatg gagcgcggag tgggggaacg     9300

ccccccagtc ctcgccggta tgtgtatacc aggctatgat cacgtgtgaa acttgggcgg     9360

acctgtatca tatgtacacc gtccctattc gtttatagcc agtacgtgtt atctgcacat     9420

agaggaacat gtgtcatact gggatcgcat gcatggtatg tgtgactcta atattattct     9480

gtatcataat aaaaacacag tgcatggtat atagaggatc gctggtaagc actacggtag     9540

accaatcggc tcagattgca ttctttggca tcgataccgt tgttaattta tatggcaaag     9600

tcttgttcat gggagatcag tatttggagg aaatatactc tggaacgatg gaaatactca     9660

aatggaatca agctaaccgc tgctattcta ttgcgcatgc aacatattac gccgactgtc     9720

ctataatcag ttctacggta ttcagaggat gccgggacgc cgttgtttat actaggcccc     9780

acagcagaat tc                                                         9792


