                         SEQUENCE LISTING

<110>  Stazione Zoologica Anton Dohrn
       FALCIATORE, Angela
       RANIELLO, Raffaella
       BOWLER, Chris
 
<120>  Algal bio-flocculation by inactivation of photoreceptors

<130>  PCT 114683

<150>  EP10187730.6
<151>  2010-10-15

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  3033
<212>  DNA
<213>  Phaeodactylum tricornutum


<220>
<221>  CDS
<222>  (1)..(3033)

<400>  1
atg agc ggg gca aat tat aga gaa gcc gac ttc cct ggt gtc cgt gcc         48
Met Ser Gly Ala Asn Tyr Arg Glu Ala Asp Phe Pro Gly Val Arg Ala           
1               5                   10                  15                

gcg ggt cgg cac aac aat tcc att aca acc aaa gag ctg acg gaa tgt         96
Ala Gly Arg His Asn Asn Ser Ile Thr Thr Lys Glu Leu Thr Glu Cys           
            20                  25                  30                    

gat cgt gag cct gtg cac ttg atc gca aac gta caa ggg ggt acc ggc        144
Asp Arg Glu Pro Val His Leu Ile Ala Asn Val Gln Gly Gly Thr Gly           
        35                  40                  45                        

cat ttg ttg ttc att cac tac ccg tct gga aaa atc ttg gct cat gat        192
His Leu Leu Phe Ile His Tyr Pro Ser Gly Lys Ile Leu Ala His Asp           
    50                  55                  60                            

cgc gac atc gaa cac att cct tgg atc cgg tgt cac gaa aac aga aca        240
Arg Asp Ile Glu His Ile Pro Trp Ile Arg Cys His Glu Asn Arg Thr           
65                  70                  75                  80            

gtt acc gct ggc cgc act ggg gcg aga act aca tct tct ttc cat gga        288
Val Thr Ala Gly Arg Thr Gly Ala Arg Thr Thr Ser Ser Phe His Gly           
                85                  90                  95                

gaa cag cag agt ggt gag agt cct cac gaa gct att ggc ata tct gga        336
Glu Gln Gln Ser Gly Glu Ser Pro His Glu Ala Ile Gly Ile Ser Gly           
            100                 105                 110                   

ggc ttt tta ctg aac tgg gtt ccg cac gat ttc tac gag aag att ctc        384
Gly Phe Leu Leu Asn Trp Val Pro His Asp Phe Tyr Glu Lys Ile Leu           
        115                 120                 125                       

gat ttg gtc ctc ggt att atc cat tcc gat acg cac aga aat ttt tat        432
Asp Leu Val Leu Gly Ile Ile His Ser Asp Thr His Arg Asn Phe Tyr           
    130                 135                 140                           

ttt tat tca tat gat ggt tca gcg tac gct att tct att tca gcg acg        480
Phe Tyr Ser Tyr Asp Gly Ser Ala Tyr Ala Ile Ser Ile Ser Ala Thr           
145                 150                 155                 160           

gaa atg gac tac tcc gtg att ggc atc gaa att gaa aca gtt ggt ttg        528
Glu Met Asp Tyr Ser Val Ile Gly Ile Glu Ile Glu Thr Val Gly Leu           
                165                 170                 175               

gat gat act gcc tcc cat ttt tca tcc tca ttg ttg cat ttg gga cgt        576
Asp Asp Thr Ala Ser His Phe Ser Ser Ser Leu Leu His Leu Gly Arg           
            180                 185                 190                   

att gtg gaa ttc tac cag cac gaa gca att gcc aag aca gcc tgt gac        624
Ile Val Glu Phe Tyr Gln His Glu Ala Ile Ala Lys Thr Ala Cys Asp           
        195                 200                 205                       

act gtt ttt cac cta ttg gga aag tat gac agg ggc atg gtg tac cga        672
Thr Val Phe His Leu Leu Gly Lys Tyr Asp Arg Gly Met Val Tyr Arg           
    210                 215                 220                           

ttc cac gat gat ctg tcc ggc gag gtc gtg cac gag att aaa gca aat        720
Phe His Asp Asp Leu Ser Gly Glu Val Val His Glu Ile Lys Ala Asn           
225                 230                 235                 240           

cat gtg gaa tcc agc tat ctt ggc atg cga ttt cct tcc tct gat att        768
His Val Glu Ser Ser Tyr Leu Gly Met Arg Phe Pro Ser Ser Asp Ile           
                245                 250                 255               

cct ttg cca tcg cga cag ctt tat ata aaa aat ggt gtg cgg tac att        816
Pro Leu Pro Ser Arg Gln Leu Tyr Ile Lys Asn Gly Val Arg Tyr Ile           
            260                 265                 270                   

tac gac gtt gat acc gag gat cta ccg att tta tcc ctg gac aat gaa        864
Tyr Asp Val Asp Thr Glu Asp Leu Pro Ile Leu Ser Leu Asp Asn Glu           
        275                 280                 285                       

aag atg gat ctc agt caa att cgc atg cgt gct gta gcc aaa ccg cat        912
Lys Met Asp Leu Ser Gln Ile Arg Met Arg Ala Val Ala Lys Pro His           
    290                 295                 300                           

att gtg tac tta aga aat atg ggg gtg gtg tcg tcg ttg agc ttg gcg        960
Ile Val Tyr Leu Arg Asn Met Gly Val Val Ser Ser Leu Ser Leu Ala           
305                 310                 315                 320           

att gtt gtc gac aat gat ctg tgg ggg ttg ctg gct ttt cat ggg tac       1008
Ile Val Val Asp Asn Asp Leu Trp Gly Leu Leu Ala Phe His Gly Tyr           
                325                 330                 335               

ggc gcg agg tac aag cct tcg ctc cat cag cga att gct tgt gaa acc       1056
Gly Ala Arg Tyr Lys Pro Ser Leu His Gln Arg Ile Ala Cys Glu Thr           
            340                 345                 350                   

ata agt gcg atg gtc tca gtt cgt att gaa tct ctc atg aaa aag gcg       1104
Ile Ser Ala Met Val Ser Val Arg Ile Glu Ser Leu Met Lys Lys Ala           
        355                 360                 365                       

cag agt gcc cga att att aag ttg ggc cag tgc act atg agc tta aag       1152
Gln Ser Ala Arg Ile Ile Lys Leu Gly Gln Cys Thr Met Ser Leu Lys           
    370                 375                 380                           

cat gac cag agc ctg att cac aat ctc tat gaa tgg ggt gaa ggc ata       1200
His Asp Gln Ser Leu Ile His Asn Leu Tyr Glu Trp Gly Glu Gly Ile           
385                 390                 395                 400           

ctc gaa att gtt gat gga gat gtt ttg gtt gca cat ata caa gat cct       1248
Leu Glu Ile Val Asp Gly Asp Val Leu Val Ala His Ile Gln Asp Pro           
                405                 410                 415               

aga gat ggc gaa ggc gac aga att gtg ctg ggt gat cct ttg ttg gta       1296
Arg Asp Gly Glu Gly Asp Arg Ile Val Leu Gly Asp Pro Leu Leu Val           
            420                 425                 430                   

ccg aag gat tct ttt tgg act aag atg agt tcc tat cag aat cgc gaa       1344
Pro Lys Asp Ser Phe Trp Thr Lys Met Ser Ser Tyr Gln Asn Arg Glu           
        435                 440                 445                       

ctc tgt gtc att tca aca cgc aaa gct ctc aca gat atc aaa ttg aca       1392
Leu Cys Val Ile Ser Thr Arg Lys Ala Leu Thr Asp Ile Lys Leu Thr           
    450                 455                 460                           

caa gaa gag tgc cca gca agt gga att gta ttt ttc caa gag ggt cgt       1440
Gln Glu Glu Cys Pro Ala Ser Gly Ile Val Phe Phe Gln Glu Gly Arg           
465                 470                 475                 480           

act cag atc atg att gga cga gca atg cga tcc aaa gat gtc gta tgg       1488
Thr Gln Ile Met Ile Gly Arg Ala Met Arg Ser Lys Asp Val Val Trp           
                485                 490                 495               

gca ggt aat cct gac gaa cca aaa cta agg att gga gga att ttg aat       1536
Ala Gly Asn Pro Asp Glu Pro Lys Leu Arg Ile Gly Gly Ile Leu Asn           
            500                 505                 510                   

ccg cgc aac tcc ttt act caa ttc att gaa aaa gcg cga aag gaa tca       1584
Pro Arg Asn Ser Phe Thr Gln Phe Ile Glu Lys Ala Arg Lys Glu Ser           
        515                 520                 525                       

cga gcc tgg act gtg caa gat att agt gtg att tct gtg ctt cgt gac       1632
Arg Ala Trp Thr Val Gln Asp Ile Ser Val Ile Ser Val Leu Arg Asp           
    530                 535                 540                           

cgt ata tgt gag cat tcg tac gca tac atg atg gga tta ctg aga ggt       1680
Arg Ile Cys Glu His Ser Tyr Ala Tyr Met Met Gly Leu Leu Arg Gly           
545                 550                 555                 560           

gat att caa gat gca aac cgg aaa tat ttg gcg gca att gac aga gcg       1728
Asp Ile Gln Asp Ala Asn Arg Lys Tyr Leu Ala Ala Ile Asp Arg Ala           
                565                 570                 575               

cgg gac aat tac gaa ttc ttt gcg cat atg agc cac gaa cta cgg act       1776
Arg Asp Asn Tyr Glu Phe Phe Ala His Met Ser His Glu Leu Arg Thr           
            580                 585                 590                   

cct ttc cat ggc gtt atg gga tgc tta agt att ctg cat gag tca att       1824
Pro Phe His Gly Val Met Gly Cys Leu Ser Ile Leu His Glu Ser Ile           
        595                 600                 605                       

gaa gat atg cca gca gcg gaa gtc aga gat gtt gtc gat aca gca ata       1872
Glu Asp Met Pro Ala Ala Glu Val Arg Asp Val Val Asp Thr Ala Ile           
    610                 615                 620                           

gct tcc gga aac cac atg atc aat ctt ctc aac gat att ctg gac atc       1920
Ala Ser Gly Asn His Met Ile Asn Leu Leu Asn Asp Ile Leu Asp Ile           
625                 630                 635                 640           

tcg aag aac aaa cac ttg tct cat ata tcg gcg cag gat aag gtt att       1968
Ser Lys Asn Lys His Leu Ser His Ile Ser Ala Gln Asp Lys Val Ile           
                645                 650                 655               

tac cag act tta gcc ttt gag aca att gac tgt atg aag tca ctg gcc       2016
Tyr Gln Thr Leu Ala Phe Glu Thr Ile Asp Cys Met Lys Ser Leu Ala           
            660                 665                 670                   

acg tcg cga aag atc gag atg aga tcg tca atc gag ccg aaa ggt ttg       2064
Thr Ser Arg Lys Ile Glu Met Arg Ser Ser Ile Glu Pro Lys Gly Leu           
        675                 680                 685                       

gaa aaa gtg gtg att gtg acg gat cgt aca aaa att att caa atc gtt       2112
Glu Lys Val Val Ile Val Thr Asp Arg Thr Lys Ile Ile Gln Ile Val           
    690                 695                 700                           

tcc aac gtt gtg aac aat gcc atc aag ttt acg ggt gaa ggg act gtc       2160
Ser Asn Val Val Asn Asn Ala Ile Lys Phe Thr Gly Glu Gly Thr Val           
705                 710                 715                 720           

gat gtt gta ttt agg ctc gtt gat tcg ctg caa gag gca act atg atg       2208
Asp Val Val Phe Arg Leu Val Asp Ser Leu Gln Glu Ala Thr Met Met           
                725                 730                 735               

tgg gag cga ggc gcg gaa gtt cat gct gga tca gtg ttt tcg atg aag       2256
Trp Glu Arg Gly Ala Glu Val His Ala Gly Ser Val Phe Ser Met Lys           
            740                 745                 750                   

gag agt gaa atg cac aca tcg gct gaa gaa gta aga cgg agc acc atg       2304
Glu Ser Glu Met His Thr Ser Ala Glu Glu Val Arg Arg Ser Thr Met           
        755                 760                 765                       

acg ttt aat gag acg cat gat caa aag tgg atg aca atg agt gtc tca       2352
Thr Phe Asn Glu Thr His Asp Gln Lys Trp Met Thr Met Ser Val Ser           
    770                 775                 780                           

gac acc gga tgc ggt atg gag ccg tct gaa cta gta gaa atg ttc tca       2400
Asp Thr Gly Cys Gly Met Glu Pro Ser Glu Leu Val Glu Met Phe Ser           
785                 790                 795                 800           

cca tat acc caa tcg agt cat gga tcc aat cgc att ttt cag gga aca       2448
Pro Tyr Thr Gln Ser Ser His Gly Ser Asn Arg Ile Phe Gln Gly Thr           
                805                 810                 815               

ggg ctt ggg ctt ttc att tgc gtt tca tta tgt tac caa ctc aat ggt       2496
Gly Leu Gly Leu Phe Ile Cys Val Ser Leu Cys Tyr Gln Leu Asn Gly           
            820                 825                 830                   

ttt att tct tgt gcg agc acc ccc gat aaa gga aca ctt ttt cat atg       2544
Phe Ile Ser Cys Ala Ser Thr Pro Asp Lys Gly Thr Leu Phe His Met           
        835                 840                 845                       

gga atc cca gtc gga ttg tta gct gaa gac aca gtt gag gga aat cag       2592
Gly Ile Pro Val Gly Leu Leu Ala Glu Asp Thr Val Glu Gly Asn Gln           
    850                 855                 860                           

aca cta aca gat gat acg aag gaa aca gag agc gtg atc caa atg tcg       2640
Thr Leu Thr Asp Asp Thr Lys Glu Thr Glu Ser Val Ile Gln Met Ser           
865                 870                 875                 880           

ggt ccg att ttg atc gta gat gac aat gtt gtg aac gtg aaa att cta       2688
Gly Pro Ile Leu Ile Val Asp Asp Asn Val Val Asn Val Lys Ile Leu           
                885                 890                 895               

aac cgg gcg cta ctt ttg gat att aga aga gct ggt ctt gca ata gag       2736
Asn Arg Ala Leu Leu Leu Asp Ile Arg Arg Ala Gly Leu Ala Ile Glu           
            900                 905                 910                   

gtc ctc aca gca ggg ggt ggg gct gaa ggt gtc cag gtc ttt cga gac       2784
Val Leu Thr Ala Gly Gly Gly Ala Glu Gly Val Gln Val Phe Arg Asp           
        915                 920                 925                       

aag cgc ccc agt cta tgc att atc gac tat cac atg ccc gat gtc gat       2832
Lys Arg Pro Ser Leu Cys Ile Ile Asp Tyr His Met Pro Asp Val Asp           
    930                 935                 940                           

ggc att gaa gcg acc tgc acc ata cgg aaa tac gag caa gaa aac aaa       2880
Gly Ile Glu Ala Thr Cys Thr Ile Arg Lys Tyr Glu Gln Glu Asn Lys           
945                 950                 955                 960           

att gat cct acc tac att ttg atg tac act gct gat gcc aca gag caa       2928
Ile Asp Pro Thr Tyr Ile Leu Met Tyr Thr Ala Asp Ala Thr Glu Gln           
                965                 970                 975               

gct aga gca ttg atc ttg agc tcc ggc gtt gac gat atc atg tcc aag       2976
Ala Arg Ala Leu Ile Leu Ser Ser Gly Val Asp Asp Ile Met Ser Lys           
            980                 985                 990                   

cct ccg ccg aag gga ttc att gcc  ggg ttg gtg cag agg  ctg cgg gtt     3024
Pro Pro Pro Lys Gly Phe Ile Ala  Gly Leu Val Gln Arg  Leu Arg Val         
        995                 1000                 1005                     

ccg gaa  tag                                                          3033
Pro Glu                                                                   
    1010                                                                  


<210>  2
<211>  1010
<212>  PRT
<213>  Phaeodactylum tricornutum

<400>  2

Met Ser Gly Ala Asn Tyr Arg Glu Ala Asp Phe Pro Gly Val Arg Ala 
1               5                   10                  15      


Ala Gly Arg His Asn Asn Ser Ile Thr Thr Lys Glu Leu Thr Glu Cys 
            20                  25                  30          


Asp Arg Glu Pro Val His Leu Ile Ala Asn Val Gln Gly Gly Thr Gly 
        35                  40                  45              


His Leu Leu Phe Ile His Tyr Pro Ser Gly Lys Ile Leu Ala His Asp 
    50                  55                  60                  


Arg Asp Ile Glu His Ile Pro Trp Ile Arg Cys His Glu Asn Arg Thr 
65                  70                  75                  80  


Val Thr Ala Gly Arg Thr Gly Ala Arg Thr Thr Ser Ser Phe His Gly 
                85                  90                  95      


Glu Gln Gln Ser Gly Glu Ser Pro His Glu Ala Ile Gly Ile Ser Gly 
            100                 105                 110         


Gly Phe Leu Leu Asn Trp Val Pro His Asp Phe Tyr Glu Lys Ile Leu 
        115                 120                 125             


Asp Leu Val Leu Gly Ile Ile His Ser Asp Thr His Arg Asn Phe Tyr 
    130                 135                 140                 


Phe Tyr Ser Tyr Asp Gly Ser Ala Tyr Ala Ile Ser Ile Ser Ala Thr 
145                 150                 155                 160 


Glu Met Asp Tyr Ser Val Ile Gly Ile Glu Ile Glu Thr Val Gly Leu 
                165                 170                 175     


Asp Asp Thr Ala Ser His Phe Ser Ser Ser Leu Leu His Leu Gly Arg 
            180                 185                 190         


Ile Val Glu Phe Tyr Gln His Glu Ala Ile Ala Lys Thr Ala Cys Asp 
        195                 200                 205             


Thr Val Phe His Leu Leu Gly Lys Tyr Asp Arg Gly Met Val Tyr Arg 
    210                 215                 220                 


Phe His Asp Asp Leu Ser Gly Glu Val Val His Glu Ile Lys Ala Asn 
225                 230                 235                 240 


His Val Glu Ser Ser Tyr Leu Gly Met Arg Phe Pro Ser Ser Asp Ile 
                245                 250                 255     


Pro Leu Pro Ser Arg Gln Leu Tyr Ile Lys Asn Gly Val Arg Tyr Ile 
            260                 265                 270         


Tyr Asp Val Asp Thr Glu Asp Leu Pro Ile Leu Ser Leu Asp Asn Glu 
        275                 280                 285             


Lys Met Asp Leu Ser Gln Ile Arg Met Arg Ala Val Ala Lys Pro His 
    290                 295                 300                 


Ile Val Tyr Leu Arg Asn Met Gly Val Val Ser Ser Leu Ser Leu Ala 
305                 310                 315                 320 


Ile Val Val Asp Asn Asp Leu Trp Gly Leu Leu Ala Phe His Gly Tyr 
                325                 330                 335     


Gly Ala Arg Tyr Lys Pro Ser Leu His Gln Arg Ile Ala Cys Glu Thr 
            340                 345                 350         


Ile Ser Ala Met Val Ser Val Arg Ile Glu Ser Leu Met Lys Lys Ala 
        355                 360                 365             


Gln Ser Ala Arg Ile Ile Lys Leu Gly Gln Cys Thr Met Ser Leu Lys 
    370                 375                 380                 


His Asp Gln Ser Leu Ile His Asn Leu Tyr Glu Trp Gly Glu Gly Ile 
385                 390                 395                 400 


Leu Glu Ile Val Asp Gly Asp Val Leu Val Ala His Ile Gln Asp Pro 
                405                 410                 415     


Arg Asp Gly Glu Gly Asp Arg Ile Val Leu Gly Asp Pro Leu Leu Val 
            420                 425                 430         


Pro Lys Asp Ser Phe Trp Thr Lys Met Ser Ser Tyr Gln Asn Arg Glu 
        435                 440                 445             


Leu Cys Val Ile Ser Thr Arg Lys Ala Leu Thr Asp Ile Lys Leu Thr 
    450                 455                 460                 


Gln Glu Glu Cys Pro Ala Ser Gly Ile Val Phe Phe Gln Glu Gly Arg 
465                 470                 475                 480 


Thr Gln Ile Met Ile Gly Arg Ala Met Arg Ser Lys Asp Val Val Trp 
                485                 490                 495     


Ala Gly Asn Pro Asp Glu Pro Lys Leu Arg Ile Gly Gly Ile Leu Asn 
            500                 505                 510         


Pro Arg Asn Ser Phe Thr Gln Phe Ile Glu Lys Ala Arg Lys Glu Ser 
        515                 520                 525             


Arg Ala Trp Thr Val Gln Asp Ile Ser Val Ile Ser Val Leu Arg Asp 
    530                 535                 540                 


Arg Ile Cys Glu His Ser Tyr Ala Tyr Met Met Gly Leu Leu Arg Gly 
545                 550                 555                 560 


Asp Ile Gln Asp Ala Asn Arg Lys Tyr Leu Ala Ala Ile Asp Arg Ala 
                565                 570                 575     


Arg Asp Asn Tyr Glu Phe Phe Ala His Met Ser His Glu Leu Arg Thr 
            580                 585                 590         


Pro Phe His Gly Val Met Gly Cys Leu Ser Ile Leu His Glu Ser Ile 
        595                 600                 605             


Glu Asp Met Pro Ala Ala Glu Val Arg Asp Val Val Asp Thr Ala Ile 
    610                 615                 620                 


Ala Ser Gly Asn His Met Ile Asn Leu Leu Asn Asp Ile Leu Asp Ile 
625                 630                 635                 640 


Ser Lys Asn Lys His Leu Ser His Ile Ser Ala Gln Asp Lys Val Ile 
                645                 650                 655     


Tyr Gln Thr Leu Ala Phe Glu Thr Ile Asp Cys Met Lys Ser Leu Ala 
            660                 665                 670         


Thr Ser Arg Lys Ile Glu Met Arg Ser Ser Ile Glu Pro Lys Gly Leu 
        675                 680                 685             


Glu Lys Val Val Ile Val Thr Asp Arg Thr Lys Ile Ile Gln Ile Val 
    690                 695                 700                 


Ser Asn Val Val Asn Asn Ala Ile Lys Phe Thr Gly Glu Gly Thr Val 
705                 710                 715                 720 


Asp Val Val Phe Arg Leu Val Asp Ser Leu Gln Glu Ala Thr Met Met 
                725                 730                 735     


Trp Glu Arg Gly Ala Glu Val His Ala Gly Ser Val Phe Ser Met Lys 
            740                 745                 750         


Glu Ser Glu Met His Thr Ser Ala Glu Glu Val Arg Arg Ser Thr Met 
        755                 760                 765             


Thr Phe Asn Glu Thr His Asp Gln Lys Trp Met Thr Met Ser Val Ser 
    770                 775                 780                 


Asp Thr Gly Cys Gly Met Glu Pro Ser Glu Leu Val Glu Met Phe Ser 
785                 790                 795                 800 


Pro Tyr Thr Gln Ser Ser His Gly Ser Asn Arg Ile Phe Gln Gly Thr 
                805                 810                 815     


Gly Leu Gly Leu Phe Ile Cys Val Ser Leu Cys Tyr Gln Leu Asn Gly 
            820                 825                 830         


Phe Ile Ser Cys Ala Ser Thr Pro Asp Lys Gly Thr Leu Phe His Met 
        835                 840                 845             


Gly Ile Pro Val Gly Leu Leu Ala Glu Asp Thr Val Glu Gly Asn Gln 
    850                 855                 860                 


Thr Leu Thr Asp Asp Thr Lys Glu Thr Glu Ser Val Ile Gln Met Ser 
865                 870                 875                 880 


Gly Pro Ile Leu Ile Val Asp Asp Asn Val Val Asn Val Lys Ile Leu 
                885                 890                 895     


Asn Arg Ala Leu Leu Leu Asp Ile Arg Arg Ala Gly Leu Ala Ile Glu 
            900                 905                 910         


Val Leu Thr Ala Gly Gly Gly Ala Glu Gly Val Gln Val Phe Arg Asp 
        915                 920                 925             


Lys Arg Pro Ser Leu Cys Ile Ile Asp Tyr His Met Pro Asp Val Asp 
    930                 935                 940                 


Gly Ile Glu Ala Thr Cys Thr Ile Arg Lys Tyr Glu Gln Glu Asn Lys 
945                 950                 955                 960 


Ile Asp Pro Thr Tyr Ile Leu Met Tyr Thr Ala Asp Ala Thr Glu Gln 
                965                 970                 975     


Ala Arg Ala Leu Ile Leu Ser Ser Gly Val Asp Asp Ile Met Ser Lys 
            980                 985                 990         


Pro Pro Pro Lys Gly Phe Ile Ala  Gly Leu Val Gln Arg  Leu Arg Val 
        995                 1000                 1005             


Pro Glu  
    1010 


<210>  3
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  3
actgaatcca gctatcttgg catgc                                             25


<210>  4
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  4
acttctagat cattgtcgac aacaat                                            26


<210>  5
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  5
gattctagag ctcatagtgc actggc                                            26


<210>  6
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  6
agtccgcgga atctcgccta ttcatg                                            26


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  7
gcgtacgctg aaccatcata                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  8
gttccgcacg atttctacga                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  9
gaattgtgct gggtgatcct                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  10
gtgagagctt tgcgtgttga                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  11
gacatcgggc atgtgatagt                                                   20


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  12
gcaatagagg tcctcacagc a                                                 21


<210>  13
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  13
gtgcaagaga ccggacatac c                                                 21


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic primer

<400>  14
cgaagtcaac caggaaacca a                                                 21


<210>  15
<211>  437
<212>  DNA
<213>  Phaeodactylum tricornutum

<400>  15
aatctcgcct attcatggtg tataaaagtt caacatccaa agctagaact tttggaaaga       60

gaaagaatat ccgaataggg cacggcgtgc cgtattgttg gagtggacta gcagaaagtg      120

aggaaggcac aggatgagtt ttctcgagac acatagcttc agcgtcgtgt aggctaggca      180

gaggtgagtt ttctcgagac ataccttcag cgtcgtcttc actgtcacag tcaactgaca      240

gtaatcgttg atccggagag attcaaaatt caatctgttt ggacctggat aagacacaag      300

agcgacatcc tgacatgaac gccgtaaaca gcaaatcctg gttgaacacg tatccttttg      360

ggggcctccg ctacgacgct cgctccagct ggggcttcct tactatacac agcgcgcata      420

tttcacggtt gccagaa                                                     437


<210>  16
<211>  650
<212>  DNA
<213>  Phaeodactylum tricornutum

<400>  16
gcaatctcac gcaccaggcg ctggaagggc aacttgcgga tgagaaggtc cgtggacttc       60

tggtaacgac ggatctcacg cagagcgacg gttccagggc gataacggtg gggcttcttg      120

actcctccgg tagccggagc ggacttgcgg gcagccttgg tggcaagctg cttgcgcggc      180

gctttgcctc cggtggattt acgggcggtt tgcttggttc gggccatttt gacggttttt      240

tttacaagag aagagttctt gaaatttgtg aggttaaagt gtgtggcttc cgccgtagtc      300

aaggagcgtg cggttgccga tcgcaccggt acgttctgta gaaatgaaca cagtgtgttg      360

aattgaaagt atggcgcagg tatggtgtgt gataagtagc agccgcgccg agacaaacaa      420

actttggttt ctacgacaat ctctgtagac aagtactaga aacccgtttg aacgagcata      480

aatctgcacc ggcaggccac cagacatcgt ttcaacgtaa tattctacgt aaccatttta      540

tcccaggaaa cctacggcct gtgaaccacc gagacggagc actcacaatt cgctctcggc      600

aacaaccgac aatcgtctta ctcacagtca ataccgaaaa caaacaacag                 650


<210>  17
<211>  953
<212>  PRT
<213>  Thalassiosira pseudonana

<400>  17

Met Ile Gly Lys Pro Leu Gly Leu Ile Phe Gly Ala Glu His Val Ser 
1               5                   10                  15      


Gln Val Lys Asp Val Ile Glu Asn Val Ile Met Thr Ser Gly Gly Gly 
            20                  25                  30          


Gly Gly Ser Ala Ser Ser Ser Gly Glu Arg His Ser Leu Pro Ser Phe 
        35                  40                  45              


Ile Gln Pro Pro Ser Lys Arg Arg Val Ser Asp Asp Asn Phe Leu Leu 
    50                  55                  60                  


Arg Ser Glu Ser Thr Leu Ser Leu Ser Gly Arg Ile Ser Cys Ser Val 
65                  70                  75                  80  


Leu Pro Ser Ser Gln Asn Phe Leu Leu Glu Leu Glu Lys Thr Pro Leu 
                85                  90                  95      


Leu Glu Lys Tyr Thr Gln Phe Glu Asp Arg Asp Val Met Ser Phe Met 
            100                 105                 110         


Glu Glu Ile Ala Lys Glu Leu Arg Ala Cys Trp Ser Ile Glu Glu Met 
        115                 120                 125             


Ala Ser Leu Val Cys Ala Lys Val Met Gln Glu Thr Pro Tyr Asp Arg 
    130                 135                 140                 


Gly Met Val Tyr Lys Phe Asp His Glu Asp Cys Gly Glu Val Val Tyr 
145                 150                 155                 160 


Glu Ala Phe Arg Ser Asp Ala Ser Glu Ala Cys Arg Lys Asp Ser Phe 
                165                 170                 175     


Leu Gly Leu Arg Phe Pro Ala Ser Asp Ile Pro Arg Gln Ala Arg Glu 
            180                 185                 190         


Leu Phe Met Arg Asn Thr Leu Arg Val Val Tyr Asp Val Asp Gly Asn 
        195                 200                 205             


Asp Phe Glu Leu Tyr Pro Pro Met Val Asp Ile Lys Arg Ala Ser Gly 
    210                 215                 220                 


Glu Lys Glu Leu Gly Tyr Thr Asp Leu Ser Met Cys Arg Leu Arg Gly 
225                 230                 235                 240 


Ser Ser Phe Val His Leu Lys Tyr Leu Lys Asn Met Gly Val Thr Ser 
                245                 250                 255     


Thr Met Val Ile Ala Ile Ile Val Asn Gly Arg Leu Trp Gly Leu Tyr 
            260                 265                 270         


Ser Phe His Gly Tyr Arg Glu Pro Leu Val Pro Ser Ala Arg Thr Arg 
        275                 280                 285             


Phe Leu Cys Glu Met Ala Ser Ile Thr Thr Ser Ile Ile Met Glu Ser 
    290                 295                 300                 


Leu Thr Arg Lys Glu Ser Asn Glu Arg Leu Met Ser Leu Asp Ser Leu 
305                 310                 315                 320 


Met Asn Ser Leu Gln Thr Thr Ser Leu Ser Asn Phe Val Glu Thr Asn 
                325                 330                 335     


Leu Ser Asp Ile Met Gly Ala Leu Gln Val Asn Leu Ile Ser Phe Arg 
            340                 345                 350         


Val Arg Asp Pro Pro Ala Thr Pro Glu Ile Lys Thr Phe Leu Asp Glu 
        355                 360                 365             


Thr Asp Gly Lys Met Glu Ser Pro Asp Glu Ile Thr Asn Glu Val Phe 
    370                 375                 380                 


Asp Ser Leu Val Glu Thr Tyr Gly Ser Val Cys Arg Asp Tyr Gly Val 
385                 390                 395                 400 


Val Tyr Ile Asp Asp Gln Lys Ser Asn Thr Leu Leu Val Asn Arg Gly 
                405                 410                 415     


Ile His Thr Leu Val Phe Phe Arg Thr Ser Gly Val Asp Val Ile Leu 
            420                 425                 430         


Ser Arg Asn Arg Thr Ile Glu Arg Val Thr Trp Gly Gly Asp Pro Asp 
        435                 440                 445             


Lys Gln Leu Glu Pro Asp Gly Thr Leu Thr Pro Arg Asn Ser Phe Ala 
    450                 455                 460                 


Ala Tyr Val Glu Asp His Phe Lys Lys Gly Lys Pro Trp Asp Ala Thr 
465                 470                 475                 480 


Asp Arg Gln Leu Ile Ser Arg Phe Ser Asp Gln Leu Glu Lys His Arg 
                485                 490                 495     


Thr Lys Glu Leu Asn Leu Glu His Ser Lys Thr Ile Lys Thr Leu Glu 
            500                 505                 510         


Lys Glu Lys Ala Glu Ala Thr Glu Thr Ala Arg Val Asn Phe Asp Phe 
        515                 520                 525             


Phe Ala His Met Ala His Glu Leu Arg Thr Pro Phe His Gly Met Val 
    530                 535                 540                 


Gly Ser Leu Glu Ala Met Arg Glu Asp Pro Val Leu Arg Asn Asn Glu 
545                 550                 555                 560 


Met Leu Lys Thr Ala Glu Leu Cys Gly Lys Ser Met Ile Lys Ile Leu 
                565                 570                 575     


Asp Asp Ile Leu Leu Val Ala Lys Gly Ser Tyr Asp Leu Gln Ile Glu 
            580                 585                 590         


Glu His Leu Phe Asp Leu Lys Ser Phe Val Lys Ile Thr Ala Asn Asp 
        595                 600                 605             


Met Ser Ser Phe Ala Leu Met Glu Gly Ala Pro Ile Gln Leu Gly Lys 
    610                 615                 620                 


Glu Leu Ile Phe Cys Lys Asp Leu Val Gly Asp Ser Gln Arg Ile Arg 
625                 630                 635                 640 


Gln Val Met Asn Asn Leu Leu Ser Asn Ala Ile Lys Phe Ser Glu Asp 
                645                 650                 655     


Ser Ile Ser Leu Asp Val Val Gln Lys Asn Ser Phe Ser Glu Val Leu 
            660                 665                 670         


Ala Val Trp Lys Thr Tyr Ser Asn Val Tyr Pro Asn Cys Glu Pro Thr 
        675                 680                 685             


Leu Leu Glu Leu Ser Thr Phe Val Ala Glu Ala Ser Glu Ala Ser Val 
    690                 695                 700                 


Asp Thr Ile Trp Val Ile Phe Ser Ile Val Asp Lys Gly Ile Gly Ile 
705                 710                 715                 720 


Thr Gly Pro Asp Leu Lys Lys Leu Gly Thr Ala Phe Thr Gln Leu Ser 
                725                 730                 735     


Ser Gly Arg Gln Lys Lys Tyr Gln Gly Thr Gly Leu Gly Leu Asn Ile 
            740                 745                 750         


Cys Asn Met Leu Val Gly Ala Leu Gly Gly Lys Leu Val Met Phe Ser 
        755                 760                 765             


Ala Lys Gly Leu Gly Ser Cys Phe Thr Phe Ala Val Pro Val Lys Lys 
    770                 775                 780                 


Ser Asp Val Pro Val Ala Glu Pro Glu Val Glu Glu Ser Gln Asn Glu 
785                 790                 795                 800 


Lys Ala Lys Lys Met Glu Ala Leu Gln Glu Glu Phe Asp Ala Phe Gly 
                805                 810                 815     


Phe Arg Glu Lys Gly Val Arg Ile Phe Val Val Asp Asp Ser Ala Ile 
            820                 825                 830         


Asn Arg Lys Leu Cys Gly Arg Lys Ile Arg Arg Trp Met Pro Ser Val 
        835                 840                 845             


Thr Ile Lys Glu Cys Ser Ser Gly Ser Ser Ala Leu Ala Glu Tyr Glu 
    850                 855                 860                 


Lys Ser Pro Lys Asp Val Met Gly Ile Phe Met Asp Tyr His Met Gln 
865                 870                 875                 880 


Asp Met Asp Gly Asp Glu Cys Thr Arg Arg Ile Arg Glu Cys Glu Ser 
                885                 890                 895     


Met Arg Glu Asp Leu Pro Asn Val Tyr Ile Val Gly Tyr Thr Ala Asp 
            900                 905                 910         


Val Leu Glu Asp Ser Thr Gly Arg Leu Met Glu Ser Gly Met Asn Ser 
        915                 920                 925             


Val Met Pro Lys Pro Glu Pro Pro Arg Gly Glu Cys Asp Ser Lys Leu 
    930                 935                 940                 


Ser Ile Arg Lys Thr Ser Arg Pro Leu 
945                 950             


